Whitening and anti-aging composition, preparation method and whitening essence containing composition

Abstract

The invention belongs to the field of biological fermentation engineering and skin health, and particularly relates to a whitening and anti-aging composition, a preparation method and whitening essence containing the composition, and the whitening and anti-aging composition is characterized by comprising the following components in percentage by weight: 95% of fermentation liquor including roxburgh rose and bolete, and the fermentation liquor can provide polyphenol, polysaccharide and amino acid substrates; the carbon source comprises honey and fructo-oligosaccharide and provides nutrition for the strain; according to the invention, no animal-derived or animal-extracted substances are added, so that ethical disputes caused by the use of animal raw materials are fundamentally avoided. Traditional products depend on animal extracted components, such as collagen and the like. The product takes an innovative plant raw material and microbial fermentation technology as a core, opens up a brand new path without participation of animal components, and accords with the pursuit of modern consumers for green and sustainable products.

Description

Technical Field

[0001] The invention belongs to the field of biological fermentation engineering and skin health, and specifically relates to a whitening and anti-aging composition, a preparation method and a whitening essence containing the composition. Background Art

[0002] Currently, whitening and anti-aging products are very popular in the market. Mainstream products usually use peptides (such as skin active peptides and collagen peptides) as core ingredients, and combine them with plant extracts (especially plant extracts with Chinese characteristics), biosynthetic ingredients (such as NMN, SOD) and hyaluronic acid, etc., focusing on achieving anti-wrinkle, repair and whitening effects.

[0003] In terms of processing technology, there are mainly the following methods: first, using microbial fermentation technology to produce biopolysaccharides or degradable materials; second, using traditional grinding or water extraction methods; third, by precisely controlling specific reaction conditions, such as controlling the temperature at 60-120°C, preparing three-dimensional polymers, thereby optimizing the stability of active ingredients.

[0004] However, existing technologies and products have many defects. In terms of ingredient absorption, large molecular active ingredients, such as traditional collagen peptides (molecular weight > 500 Daltons), are extremely difficult to be absorbed by the skin, which greatly limits the anti-aging effect. In terms of ingredient safety, some products add high concentrations of retinol, alcohol and other irritating ingredients in pursuit of efficacy, which can easily cause adverse reactions such as damaged skin barriers and allergies. From the perspective of active ingredient stability, traditional active ingredients such as SOD have a very short half-life in the aqueous medium commonly used in cosmetics, often only a few months, and are very easy to inactivate. In terms of raw material sources, some key raw materials, such as collagen, are mainly extracted from animals, which not only faces ethical controversy, but also has a high risk of allergies, bringing many challenges to the sustainable development of products and consumer experience.

[0005] In addition, the existing constant temperature fermentation still has some problems: the direct use of roxburghii juice is easy to oxidize, the utilization rate of active ingredients is low, and the traditional fermentation process is difficult to simultaneously increase the content of SOD and ergothioneine; ergothioneine in boletus is highly heat-sensitive, and conventional extraction methods lead to a high loss rate; single strain fermentation has metabolic limitations and cannot fully degrade plant fiber and release highly active ingredients;

[0006] In traditional fermentation processes, the SOD content usually does not exceed 10,000U / 100ml; the purity of ergothioneine extraction from boletus is only about 0.1mg / g.

[0007] To this end, the present invention provides a whitening and anti-aging composition, a preparation method and a whitening essence containing the composition. Summary of the invention

[0008] In order to make up for the deficiencies of the prior art, at least one technical problem raised in the background technology is solved.

[0009] The technical solution adopted by the present invention to solve the technical problem is: a whitening and anti-aging composition according to the present invention is characterized in that the whitening and anti-aging composition comprises the following components and proportions:

[0010] The fermentation liquid includes roxburghii and boletus: 95%, and the fermentation liquid can provide polyphenols, polysaccharides and amino acid substrates;

[0011] Carbon sources include honey and oligofructose: 1%, providing nutrition for the bacteria;

[0012] Lactobacillus plantarum: 1%, can decompose pectin and promote SOD synthesis;

[0013] Lactobacillus acidophilus: 1%, produces acid to regulate the fermentation environment and enhance the synergy of the bacterial flora;

[0014] Saccharomyces cerevisiae: 1%, secretes ergothioneine synthase;

[0015] Saccharomyces cerevisiae: 1%, improves the bioavailability of ergothioneine;

[0016] The bacterial species ratio of Lactobacillus plantarum, Lactobacillus acidophilus, Saccharomyces cerevisiae and Saccharomyces carpello is 1:1:1:1.

[0017] Preferably, the fermentation broth is obtained by two-stage fermentation:

[0018] The first stage is the bacterial colonization stage, which is fermented under anaerobic conditions at 34°C for 24 hours to reduce the pH to 3.8-4.0 to promote enzyme production by lactic acid bacteria;

[0019] The second stage is the product synthesis stage, which is fermented at 28° C. under aerobic conditions for 72 hours, and 0.1% yeast extract is added as a nutrient enhancer in this stage.

[0020] Preferably, the proportion of honey is 0.5%, and the proportion of oligofructose is 0.5%.

[0021] A method for preparing a whitening and anti-aging composition, the preparation method comprising the following steps:

[0022] S1. Equipment sterilization: Steam the wall-breaking machine, fermentation tank and the connected pipes and pumps and sterilize them at 121°C for 30 minutes to achieve a sterile state;

[0023] S2. Raw material processing: accurately weigh the tea brown boletus according to the formula requirements, add the tea brown boletus, roxburghii juice, and carbon source into the wall breaking machine in the ratio of 2:8:1, break the wall into a slurry, and then pump it into a sterile constant temperature fermentation tank through a pump;

[0024] S3. Bacteria domestication:

[0025] Lactobacillus plantarum and Lactobacillus acidophilus: acclimate for 3 generations using MRS medium to make them tolerate pH ≤ 3.5;

[0026] Saccharomyces cerevisiae and Saccharomyces cerevisiae: 2 generations of domestication on YPD medium increased the ergothioneine production capacity by 3 times;

[0027] S4. Inoculation of bacterial strains: inoculate Lactobacillus plantarum, Lactobacillus acidophilus, Saccharomyces cerevisiae, and Saccharomyces carpello through the inoculation port of the sterile constant temperature fermentation tank at a ratio of 1:1:1:1;

[0028] S5. Fermentation:

[0029] Stage 1: Control the temperature to 32°C, anaerobic environment, speed to 200 rpm, and ferment for 24 hours;

[0030] The second stage: the temperature was controlled at 28°C, aerobic environment, ventilation volume was 0.3vvm, and fermentation lasted for 72 hours;

[0031] S6, stop fermentation: when SOD content ≥ 200,000U / 100ml or thioneine content ≥ 0.5mg / g, stop fermentation;

[0032] S7, post-processing: centrifugation, mixing tank treatment, autoclave sterilization, and filling.

[0033] Furthermore, the carbon source in step S2 includes 0.5% honey and 0.5% oligofructose.

[0034] Furthermore, the MRS culture medium in step S3 contains 5% of roxburghii juice, and the YPD culture medium contains 10% of boletus extract.

[0035] Furthermore, the centrifugal speed in step S7 is 4000 rpm and lasts for 10 minutes, the blending tank treatment step can be seasoned or not, and the sterilization temperature of the autoclave is 115° C. and lasts for 30 minutes.

[0036] A whitening essence of a whitening and anti-aging composition, the whitening essence of the whitening and anti-aging composition comprises the following ingredients and proportions:

[0037] Rosa roxburghii fermented liquid: 14%;

[0038] Niacinamide: 0.05%;

[0039] Panthenol: 0.3%;

[0040] Glycerol: 5%-8%;

[0041] Sodium hyaluronate: 0.1%-0.3%;

[0042] Dipotassium glycyrrhizinate: 0.2%-0.5%;

[0043] Centella asiatica extract: 0.5%-1%;

[0044] 1,2-Pentanediol: 3%-5%;

[0045] Deionized water: make up the balance to 100%;

[0046] Lactic acid / citric acid: Adjust pH to 5.5-6.5.

[0047] The beneficial effects of the present invention are as follows:

[0048] 1. The whitening and anti-aging composition, preparation method and whitening essence containing the composition described in the present invention do not add any animal-derived or animal-extracted substances, which fundamentally eliminates the ethical disputes caused by the use of animal raw materials. Traditional products rely on animal-extracted ingredients, such as collagen. This product, with innovative plant raw materials and microbial fermentation technology as the core, has opened up a new path without the participation of animal ingredients, which is in line with modern consumers' pursuit of green and sustainable products.

[0049] 2. The whitening and anti-aging composition, preparation method and whitening essence containing the composition of the present invention abandon the addition of high-concentration irritating substances, greatly reducing the risks of skin barrier damage, allergies, etc. Many whitening and anti-aging products on the market add high-concentration retinol, alcohol, etc. in pursuit of efficacy. Although they may have certain effects in the short term, long-term use can easily damage the skin barrier and cause allergies, redness, swelling, dryness and other problems. This product relies on carefully formulated plant fermentation ingredients to act gently on the skin, ensuring that people with sensitive skin and other skin types can use it with peace of mind, achieving a perfect balance between safety and efficacy.

[0050] 3. The whitening and anti-aging composition, preparation method and whitening essence containing the composition of the present invention significantly improve the effective ingredient content of ergothioneine in tea brown boletus and SOD (superoxide dismutase) in roxburghii through a unique microbial constant temperature fermentation preparation process. During the fermentation process, two-stage temperature-controlled fermentation and precise strain ratio play a key role. The first stage 34 ° C anaerobic fermentation promotes lactic acid bacteria to produce enzymes, and the second stage 28 ° C aerobic fermentation and yeast extract are added to promote the ergothioneine content to 0.5 mg / g, far exceeding the initial content of boletus; at the same time, the SOD content is increased from 4500U / 100ml to 206,000U / 100ml. High content of ergothioneine can more efficiently inhibit intracellular melanin synthesis and tyrosinase activity, reducing melanin deposition; high activity SOD strongly removes free radicals and reduces cell damage, and the two work together to greatly improve the whitening and anti-aging effect.

[0051] 4. The whitening and anti-aging composition, preparation method and whitening essence containing the composition of the present invention realize the directional separation of four characteristic strains from different parts of Rosa roxburghii, and construct an efficient fermentation system. This directional separation technology makes the strain more targeted and adaptable, and plant lactobacillus, lactobacillus acidophilus, saccharomyces cerevisiae and carboro yeast each perform their duties and synergize in a specific fermentation environment. For example, plant lactobacillus and lactobacillus acidophilus form an enzyme-producing complex, decompose rosa roxburghii pectin to improve SOD release efficiency; saccharomyces cerevisiae and carboro yeast transform ergothioneine precursors through gene interaction, providing solid technical support for improving product efficacy.

[0052] 5. the whitening and anti-aging composition of the present invention, the preparation method and the whitening essence comprising the composition, realize technological breakthrough by means of pH segmentation control and nutrient supplementation strategy, so that SOD output is improved by 455 times, and the content of ergothioneine exceeds the highest value reported in the literature by 5 times. Accurate pH regulation creates a suitable environment for the bacterial metabolism in different stages, and nutrient supplementation specifically meets the nutritional needs of bacterial species in the product synthesis stage, ensures that the fermentation process is carried out efficiently, greatly improves the content of key functional ingredients, and allows products to stand out in the field of whitening and anti-aging.

[0053] 6. The whitening and anti-aging composition, preparation method and whitening essence containing the composition described in the present invention illustrate the mechanism by which bacterial metabolites (such as γ-aminobutyric acid and 4-hydroxyphenylacetaldehyde) synergistically regulate whitening and anti-aging through the "gut-skin axis". This interdisciplinary discovery expands the pathway of whitening and anti-aging, indicating that the product can not only act directly on the skin, but also indirectly affect the physiological state of the skin by regulating the intestinal microbiome and its metabolites. For example, γ-aminobutyric acid can regulate neurotransmitters and improve skin blood circulation and metabolism; 4-hydroxyphenylacetaldehyde has antioxidant and anti-inflammatory effects, achieving all-round whitening and anti-aging from the inside out, and providing a new theoretical basis and practical direction for skin health management.

[0054] 7. The whitening and anti-aging composition, preparation method and whitening essence containing the composition described in the present invention, the whitening essence containing the whitening and anti-aging composition of the present invention, by accurately adjusting the pH value to 5.5-6.5, so that it is more in line with the physiological environment of the skin, reduces irritation, and ensures the stability of the active ingredients in the composition. Adding 0.02-0.05% niacinamide and 0.1-0.3% panthenol further enhances the efficacy. Niacinamide inhibits the transfer of melanin and synergizes with ergothioneine and other substances in the composition for whitening; panthenol moisturizes and soothes, enhances the skin barrier function, so that the essence can give the skin a hydrated and healthy luster while whitening and anti-aging, thereby improving the overall user experience. DETAILED DESCRIPTION

[0055] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0056] A whitening and anti-aging composition according to an embodiment of the present invention comprises the following components and proportions:

[0057] The fermentation liquid includes roxburghii and boletus: 95%, and the fermentation liquid can provide polyphenols, polysaccharides and amino acid substrates;

[0058] Carbon sources include honey and oligofructose: 1%, providing nutrition for the bacteria;

[0059] Lactobacillus plantarum: 1%, can decompose pectin and promote SOD synthesis;

[0060] Lactobacillus acidophilus: 1%, produces acid to regulate the fermentation environment and enhance the synergy of the bacterial flora;

[0061] Saccharomyces cerevisiae: 1%, secretes ergothioneine synthase;

[0062] Saccharomyces cerevisiae: 1%, improves the bioavailability of ergothioneine;

[0063] The bacterial species ratio of Lactobacillus plantarum, Lactobacillus acidophilus, Saccharomyces cerevisiae and Saccharomyces carpello is 1:1:1:1.

[0064] The fermentation broth is obtained by two-stage fermentation:

[0065] The first stage is the bacterial colonization stage, which is fermented under anaerobic conditions at 34°C for 24 hours to reduce the pH to 3.8-4.0 to promote enzyme production by lactic acid bacteria;

[0066] The second stage is the product synthesis stage, which is fermented at 28°C under aerobic conditions for 72 hours, and 0.1% yeast extract is added as a nutritional enhancer in this stage. The ergothioneine content is increased to 0.5 mg / g.

[0067] The proportion of honey is 0.5% and the proportion of oligofructose is 0.5%.

[0068] Specifically, the synergistic mechanism of strain combination:

[0069] Lactobacillus plantarum and Lactobacillus acidophilus form an enzyme-producing complex to decompose roxburghii pectin into oligosaccharides (molecular weight <1000Da), thereby improving the efficiency of SOD release.

[0070] Saccharomyces cerevisiae and Saccharomyces carpelophyllosaccharomyces convert the ergothioneine precursor in boletus into an active form through genetic interactions (such as cross-activation of the thiamine metabolic pathway).

[0071] A method for preparing a whitening and anti-aging composition, the preparation method comprising the following steps:

[0072] S1. Equipment sterilization: Steam the wall-breaking machine, fermentation tank and the connected pipes and pumps and sterilize them at 121°C for 30 minutes to achieve a sterile state;

[0073] S2, raw material processing: accurately weigh the tea brown boletus according to the formula requirements, add the tea brown boletus, roxburghii juice, and carbon source (0.5% honey, 0.5% oligofructose) into the wall breaking machine in a ratio of 2:8:1, break the wall into a slurry, and then pump it into a sterile constant temperature fermentation tank through a pump;

[0074] S3. Bacteria domestication:

[0075] Lactobacillus plantarum and Lactobacillus acidophilus: acclimate for 3 generations using MRS medium (containing 5% roxburghii juice) to make them tolerate pH ≤ 3.5;

[0076] Saccharomyces cerevisiae and Saccharomyces carpelophyllosporin: 2 generations of domestication using YPD medium (containing 10% boletus extract) increased the ergothioneine production capacity by 3 times;

[0077] S4. Inoculation of bacterial strains: inoculate Lactobacillus plantarum, Lactobacillus acidophilus, Saccharomyces cerevisiae, and Saccharomyces carpello through the inoculation port of the sterile constant temperature fermentation tank at a ratio of 1:1:1:1;

[0078] S5. Fermentation:

[0079] Stage 1: Control the temperature to 32°C, anaerobic environment, speed to 200 rpm, and ferment for 24 hours;

[0080] The second stage: the temperature was controlled at 28°C, aerobic environment, ventilation volume was 0.3vvm, and fermentation lasted for 72 hours;

[0081] S6. Terminate fermentation: When the SOD content is ≥200,000U / 100ml or the ergothioneine content is ≥0.5mg / g, terminate fermentation;

[0082] S7, post-processing: centrifugation (4000rpm, 10 minutes), blending tank treatment (flavoring or no flavoring can be selected), autoclave sterilization (115°C, 30 minutes), and filling.

[0083] Specifically, fermentation raw materials: roxburghii juice, boletus, lactobacillus, yeast;

[0084] Source of bacteria: Lactobacillus plantarum, Lactobacillus acidophilus, Saccharomyces cerevisiae and Saccharomyces carpelosus extracted from the flowers, fruits and roots of the sea buckthorn plantation of Yuankun Pharmaceutical in Maoliping Village, Bijie, Guizhou.

[0085] Formula principle:

[0086] Rosa roxburghii, known as the "Three King Fruits" Vitamin C: The vitamin C content in every 100 grams of pulp can reach 2585 mg. Vitamin C can inhibit the activity of tyrosinase, reduce melanin production, and promote collagen synthesis to improve skin color;

[0087] SOD (superoxide dismutase): The SOD content in sea buckthorn is as high as 4500U / 100ml. SOD is an internationally recognized anti-aging active substance. It can efficiently remove free radicals, reduce the damage of free radicals to cells, and delay aging; it can inhibit the expression and activity of tyrosinase and reduce pigment deposition.

[0088] Flavonoids: The content of flavonoids in sea buckthorn is 380mg / 100g; flavonoids have antioxidant effects, can eliminate free radicals in the body and delay cell aging.

[0089] Brown Boletus: Contains 0.09656 mg / g of ergothioneine (EGT), a natural antioxidant that can inhibit intracellular melanin synthesis and tyrosinase activity, thereby reducing melanin deposition and achieving a whitening effect. At the same time, ergothioneine also has anti-aging effects.

[0090] The active ingredient of the present invention was tested, and the specific parameters of the test were:

[0091] 1. Enhanced efficacy ingredients:

[0092] SOD content: fermentation broth from 4500U / 100ml→206,000U / 100ml (HPLC detection, method: GB / T5009.171-2003 first method);

[0093] Ergothioneine content: Boletus from 0.09656 mg / g → 0.5 mg / g (HPLC detection, NY / T3872-2021).

[0094] 2. Whitening mechanism verification:

[0095] Tyrosinase inhibition: The tyrosinase inhibition rate of the fermentation broth was as high as 82% (the α-arbutin control group was 65%, p<0.01);

[0096] Antioxidant capacity: ABTS free radical scavenging rate was 89% (the vitamin C control group was 92%, with no significant difference).

[0097] 3. Stability test:

[0098] After 180 days of storage at 40℃ / RH75%, the SOD activity retention rate was 91% and the ergothioneine degradation rate was <5%.

[0099] A whitening essence of a whitening and anti-aging composition, the whitening essence of the whitening and anti-aging composition comprises the following ingredients and proportions:

[0100] Rosa roxburghii fermented liquid: 14%;

[0101] Niacinamide: 0.05%;

[0102] Panthenol: 0.3%;

[0103] Glycerol: 5%-8%;

[0104] Sodium hyaluronate: 0.1%-0.3%;

[0105] Dipotassium glycyrrhizinate: 0.2%-0.5%;

[0106] Centella asiatica extract: 0.5%-1%;

[0107] 1,2-Pentanediol: 3%-5%;

[0108] Deionized water: make up the balance to 100%;

[0109] Lactic acid / citric acid: Adjust pH to 5.5-6.5.

[0110] Specifically, the core active ingredients:

[0111] Rosa roxburghii fermented liquid: 14%, Effect: Rosa roxburghii is rich in natural VC derivatives (whitening and antioxidant), and boletus contains ergothioneine (anti-aging, anti-inflammatory and repair). After fermentation, small molecule active substances are easier to penetrate, synergistically inhibiting melanin production and promoting collagen synthesis.

[0112] Niacinamide: 0.05% (upper limit), function: inhibit the transfer of melanin to the epidermis, control oil and resist glycation.

[0113] Panthenol (vitamin B5): 0.3% (upper limit), function: deeply moisturizes and repairs the barrier, reduces photoaging damage.

[0114] Auxiliary functional ingredients:

[0115] Glycerin: 5%-8%, Function: Basic moisturizer, improves skin moisture.

[0116] Sodium hyaluronate: 0.1%-0.3%, Function: Multi-molecular weight compound, long-lasting moisture locking and skin filling.

[0117] Soothing and stabilizing system:

[0118] Dipotassium glycyrrhizinate: 0.2%-0.5%, function: anti-inflammatory, reduces redness, and reduces irritation.

[0119] Centella asiatica extract: 0.5%-1%, function: repair damaged cells and improve skin elasticity.

[0120] Corrosion protection and solvents:

[0121] 1,2-Pentanediol: 3%-5%, Function: Mild antiseptic and moisturizing, improving formula stability.

[0122] The remaining deionized water: make up to 100%.

[0123] pH adjuster: Lactic acid / citric acid: Adjust to pH 5.5-6.5. Function: Fit the weakly acidic environment of the skin and reduce irritation.

[0124] Formula design and effect description:

[0125] 1. Full coverage of whitening channels

[0126] Inhibit melanin production: Rosa roxburghii VC (reducing melanin) and niacinamide (blocking transfer) form a triple blocking mechanism.

[0127] Antioxidant protection: Boletus ergothioneine is a natural antioxidant that can inhibit intracellular melanin synthesis and tyrosinase activity, thereby reducing melanin deposition and achieving a whitening effect.

[0128] 2. Anti-aging synergy

[0129] Collagen Activation: Boletus ergothioneine can scavenge free radicals and reduce oxidative stress. Centella asiatica extract stimulates collagen synthesis.

[0130] Barrier repair: Panthenol + hyaluronic acid strengthen the stratum corneum's ability to lock in moisture, improving fine lines and dry aging.

[0131] 3. Mildness and universality

[0132] Low concentration of niacinamide (0.05%) reduces the risk of sensitivity, and dipotassium glycyrrhizinate neutralizes potential irritation, making it suitable for most skin types.

[0133] pH 5.5-6.5 maintains the balance of skin microecology and avoids barrier damage.

[0134] Example 1: Optimal fermentation parameters optimization

[0135] Bacterial species ratio: Lactobacillus plantarum (1%), Lactobacillus acidophilus (1%), Saccharomyces cerevisiae (1%), Saccharomyces cerevisiae (1%);

[0136] Fermentation results: SOD content 210,000U / 100ml, ergothioneine 0.52mg / g.

[0137] Example 2: Skin care product application

[0138] Ingredients: fermentation liquid (20%), niacinamide (0.03%), sodium hyaluronate (0.5%), PEG-40 hydrogenated castor oil (5%);

[0139] Test method: 30 volunteers used the product for 28 consecutive days, and the skin melanin index (MI) decreased by 37% (VISIA test).

[0140] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A whitening and anti-aging composition, characterized in that: The whitening and anti-aging composition comprises the following ingredients and proportions: The fermentation liquid includes roxburghii and boletus: 95%, and the fermentation liquid can provide polyphenols, polysaccharides and amino acid substrates; Carbon sources include honey and oligofructose: 1%, providing nutrition for the bacteria; Lactobacillus plantarum: 1%, can decompose pectin and promote SOD synthesis; Lactobacillus acidophilus: 1%, produces acid to regulate the fermentation environment and enhance the synergy of the bacterial flora; Saccharomyces cerevisiae: 1%, secretes ergothioneine synthase; Saccharomyces cerevisiae: 1%, improves the bioavailability of ergothioneine; The bacterial species ratio of Lactobacillus plantarum, Lactobacillus acidophilus, Saccharomyces cerevisiae and Saccharomyces carpello is 1:1:1:

1.

2. A whitening and anti-aging composition according to claim 1, characterized in that: The fermentation broth is obtained by two-stage fermentation: The first stage is the bacterial colonization stage, which is fermented under anaerobic conditions at 34°C for 24 hours to reduce the pH to 3.8-4.0 to promote enzyme production by lactic acid bacteria; The second stage is the product synthesis stage, which is fermented at 28° C. under aerobic conditions for 72 hours, and 0.1% yeast extract is added as a nutrient enhancer in this stage.

3. A whitening and anti-aging composition according to claim 1, characterized in that: The proportion of the honey is 0.5%, and the proportion of the oligofructose is 0.5%.

4. A method for preparing a whitening and anti-aging composition, characterized in that: The composition is used for preparing the whitening and anti-aging composition according to any one of claims 1 to 3, and the preparation method comprises the following steps: S1. Equipment sterilization: Steam the wall-breaking machine, fermentation tank and the connected pipes and pumps and sterilize them at 121°C for 30 minutes to achieve a sterile state; S2. Raw material processing: accurately weigh the tea brown boletus according to the formula requirements, add the tea brown boletus, roxburghii juice, and carbon source into the wall breaking machine in the ratio of 2:8:1, break the wall into a slurry, and then pump it into a sterile constant temperature fermentation tank through a pump; S3. Bacteria domestication: Lactobacillus plantarum and Lactobacillus acidophilus: acclimate for 3 generations using MRS medium to make them tolerate pH ≤ 3.5; Saccharomyces cerevisiae and Saccharomyces cerevisiae: 2 generations of domestication on YPD medium increased the ergothioneine production capacity by 3 times; S4. Inoculation of bacterial strains: inoculate Lactobacillus plantarum, Lactobacillus acidophilus, Saccharomyces cerevisiae, and Saccharomyces carpello through the inoculation port of the sterile constant temperature fermentation tank at a ratio of 1:1:1:1; S5. Fermentation: Stage 1: Control the temperature to 32°C, anaerobic environment, speed to 200 rpm, and ferment for 24 hours; The second stage: the temperature was controlled at 28°C, aerobic environment, ventilation volume was 0.3vvm, and fermentation lasted for 72 hours; S6. Terminate fermentation: When the SOD content is ≥200,000U / 100ml or the ergothioneine content is ≥0.5mg / g, terminate fermentation; S7, post-processing: centrifugation, mixing tank treatment, autoclave sterilization, and filling.

5. The method for preparing a whitening and anti-aging composition according to claim 4, characterized in that: The carbon source in step S2 includes 0.5% honey and 0.5% oligofructose.

6. The method for preparing a whitening and anti-aging composition according to claim 4, characterized in that: The MRS culture medium in step S3 contains 5% of roxburghii juice, and the YPD culture medium contains 10% of boletus extract.

7. The method for preparing a whitening and anti-aging composition according to claim 4, characterized in that: The centrifugal speed in step S7 is 4000 rpm and lasts for 10 minutes. The blending tank treatment step can be seasoned or not. The sterilization temperature of the autoclave is 115° C. and lasts for 30 minutes.

8. A whitening essence of a whitening and anti-aging composition, characterized in that: A whitening and anti-aging composition comprising any one of claims 1 to 3, wherein the whitening essence of the whitening and anti-aging composition comprises the following ingredients and proportions: Rosa roxburghii fermented liquid: 14%; Niacinamide: 0.05%; Panthenol: 0.3%; Glycerol: 5%-8%; Sodium hyaluronate: 0.1%-0.3%; Dipotassium glycyrrhizinate: 0.2%-0.5%; Centella asiatica extract: 0.5%-1%; 1,2-Pentanediol: 3%-5%; Deionized water: make up the balance to 100%; Lactic acid / citric acid: Adjust pH to 5.5-6.5.