Essential oil hoof bath lotion as well as preparation method and application thereof

Abstract

The invention discloses essential oil type hoof bath lotion as well as a preparation method and application thereof, and belongs to the technical field of health care and treatment of cattle animal hoof diseases. The essential oil hoof bath lotion comprises the following raw materials: essential oil, an antioxidant, a synergist, a preservative, an emulsifier, a solvent and a coloring agent, wherein the essential oil comprises at least two of thymol, cinnamyl aldehyde, eugenol or carvacrol. The essential oil hoof bath lotion is prepared by mixing the raw materials in sequence, then performing multi-step emulsification and finally cooling. The essential oil hoof bath lotion prepared by the invention can effectively kill a plurality of pathogenic bacteria generating hoof lesions of dairy cows; the composition also has excellent stability, is non-toxic to human, is safe to operate, has small irritation to dairy cows, and has a good hoof disease treatment effect; the environment-friendly degradation agent has the advantages of no corrosion to equipment, environment friendliness, fast degradation, no antibiotic and the like.

Description

Technical Field

[0001] The invention belongs to the technical field of health care and treatment of hoof diseases of cattle, and in particular relates to an essential oil hoof bath liquid and a preparation method and application thereof. Background Art

[0002] Hoof disease in dairy cows is a common disease in dairy farming. In severe cases, it can cause paralysis in dairy cows, eventually leading to a decrease in milk production and affecting farming performance. The factors that lead to this disease are comprehensive, including farming technology, feed ratio, feeding environment, drug use, disinfection and other aspects. The treatment after the disease occurs must also be comprehensive, and strengthening management and control from multiple angles can achieve good results.

[0003] There are many health care or treatment methods for this disease at home and abroad, and they all show certain effects. For example, Western medicine products, Chinese medicine products, disinfectant products, etc., each has its own advantages and disadvantages. For example, the common use of copper sulfate will lead to excessive heavy metals in the environment; the cost of Chinese medicine products remains high, and the effect is affected by the quality of Chinese medicinal materials; disinfectant products have defects in irritation, volatility, and timeliness.

[0004] Therefore, there is an urgent need to provide an essential oil hoof bath liquid and a preparation method thereof which is green, safe, low-cost and can effectively treat hoof diseases of cattle. Summary of the invention

[0005] In view of the above technical problems, the present invention proposes an essential oil hoof bath liquid and a preparation method and application thereof. The essential oil hoof bath liquid prepared by the present invention can effectively kill a variety of pathogens related to hoof lesions in dairy cows and other bacteria such as Staphylococcus aureus, Escherichia coli, methicillin-resistant Staphylococcus aureus and other bacteria. Compared with traditional hoof bath liquids, the essential oil hoof bath liquid prepared by the preparation method of the present invention has the advantages of good product stability, no toxicity to humans, safe operation, low irritation to dairy cows, good therapeutic effect, no corrosion to equipment, environmentally friendly, fast degradation, and no antibiotics.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] One of the technical solutions of the present invention:

[0008] An essential oil hoof bath liquid, characterized in that it comprises essential oils, wherein the essential oils comprise at least two of thymol, cinnamaldehyde, eugenol or carvacrol; and the content of the essential oils in the essential oil hoof bath liquid is 20wt.%-25wt.%.

[0009] Optionally, the essential oil hoof bath comprises the following raw materials:

[0010] Essential oils, antioxidants, synergists, preservatives, emulsifiers, solvents and colorants;

[0011] Wherein, the essential oil comprises at least two of thymol, cinnamaldehyde, eugenol or carvacrol.

[0012] Beneficial effects: The present invention applies essential oils to drugs or health products for treating hoof diseases of cattle, wherein thymol can effectively inhibit the growth and reproduction of various bacteria, has a therapeutic effect on some diseases caused by bacterial infection, and has antibacterial activity against a variety of different bacteria (including Staphylococcus aureus, Bacillus subtilis, Escherichia coli, etc.); cinnamaldehyde has an inhibitory effect on Escherichia coli, Staphylococcus aureus, Staphylococcus albus, Pneumococcus, and Clostridium difficile; eugenol has obvious antibacterial effects on a variety of bacteria such as Staphylococcus aureus, Staphylococcus albus, Proteus, Pseudomonas aeruginosa, Shigella dysenteriae, Salmonella typhi, Salmonella paratyphi, and Salmonella; eugenol also has inhibitory activity against tinea and deep fungi caused by some fungi. Carvacrol also has antioxidant, antibacterial, and preservative effects. The present invention uses the above two or more essential oil components in a compounded manner, which has a synergistic antibacterial effect and has a significant effect on treating hoof diseases of cattle.

[0013] Optionally, the essential oil hoof bath comprises the following raw materials in percentage by weight:

[0014] Essential oil 20%-25%, antioxidant + synergist 0.2%-0.5%, preservative 0.2%-0.5%, emulsifier 60%-75%, colorant 3%-5%, the balance is solvent.

[0015] Furthermore, the mass ratio of the antioxidant to the synergist is 6:4.

[0016] Optionally, the antioxidant includes: at least one of tert-butylhydroquinone (TBHQ), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT); and / or

[0017] The synergist comprises at least one of citric acid and vitamin C; and / or

[0018] The preservative is potassium sorbate; and / or

[0019] The emulsifier is polyoxyethylene sorbitan monooleate, i.e. polysorbate 80; and / or

[0020] The solvent is purified water.

[0021] Furthermore, the essential oil hoof bath comprises the following raw materials in percentage by weight:

[0022] Thymol 7%, cinnamaldehyde 10%, eugenol 5%, tert-butylhydroquinone (TBHQ) + enhancer (citric acid) 0.2%, potassium sorbate 0.2%, polysorbate-80 65%, colorant 3%, and the balance is purified water.

[0023] The second technical solution of the present invention:

[0024] A method for preparing an essential oil hoof bath liquid comprises the following steps:

[0025] Weigh the raw materials according to the above amounts for later use;

[0026] The essential oil, antioxidant and synergist are mixed and heated to make them all melt; then an emulsifier is added thereto to perform the first step of emulsification to obtain a mixture;

[0027] The preservative is dissolved in a solvent and then added into the mixture for the second step of emulsification, and then a colorant is added for the third step of emulsification, and then the emulsification is stopped, the mixture is allowed to stand, and cooled to obtain the essential oil hoof bath liquid.

[0028] Optionally, the temperature during the heating process is 70°C-90°C.

[0029] Optionally, the rotation speed during the first emulsification step is ∼900 rpm;

[0030] The conditions in the second and third emulsification steps are: rotation speed of 1200 rpm, temperature of 55° C., and emulsification time of 15 minutes.

[0031] Optionally, the standing time is 1-2 hours.

[0032] The third technical solution of the present invention:

[0033] The essential oil hoof bath liquid is used in preparing medicines or health products for treating hoof diseases of cattle.

[0034] Compared with the prior art, the present invention has the following advantages and technical effects:

[0035] 1. Copper sulfate, formaldehyde and some disinfectants are widely used at home and abroad. Although these products are cheap, they have some side effects on the environment and breeders, especially cows, such as non-degradability, irritation, corrosion, etc. The essential oil hoof bath provided by the present invention does not have the above side effects, and compared with traditional hoof baths, it has the following advantages: good product stability, non-toxic and harmless to humans, safe operation, low irritation to cows, good effect, non-corrosive to equipment, environmentally friendly, fast degradation, and does not contain antibiotics.

[0036] 2. The essential oil hoof bath prepared by the present invention has a rapid killing effect on a variety of pathogens related to hoof lesions in dairy cows (such as Treponema corrosive, Campylobacter feces, Artiodactyla tuberculosis, Fusobacterium necroticum and other pathogens) and other bacteria, such as Staphylococcus aureus, Escherichia coli, methicillin-resistant Staphylococcus aureus and other bacteria. DETAILED DESCRIPTION

[0037] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0038] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0039] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0040] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0041] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0042] The embodiment of the present invention discloses an essential oil hoof bath liquid, which comprises the following raw materials in terms of weight percentage:

[0043] Essential oil 20%-25%, antioxidant + synergist 0.2%-0.5%, preservative 0.2%-0.5%, emulsifier 60%-75%, solvent 12%-15%, colorant 3%-5%.

[0044] In some optional embodiments, the essential oil includes at least one of thymol, cinnamaldehyde, eugenol or carvacrol.

[0045] In some optional embodiments, the antioxidant includes: at least one of tert-butylhydroquinone (TBHQ), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT);

[0046] The enhancer includes at least one of citric acid and vitamin C.

[0047] In some alternative embodiments, the preservative is potassium sorbate.

[0048] In some optional embodiments, the emulsifier is polyoxyethylene sorbitan monooleate, ie, polysorbate-80.

[0049] In some alternative embodiments, the solvent is purified water.

[0050] In some optional embodiments, the following raw materials are included, calculated by weight: 22% essential oil, 0.2% antioxidant + synergist, 0.2% preservative, 65% emulsifier, 3% colorant, and the rest is solvent.

[0051] In some optional embodiments, the following raw materials are included, measured by weight: 7% thymol, 10% cinnamaldehyde, 5% eugenol, 0.2% tert-butylhydroquinone (TBHQ) + enhancer (citric acid), 0.2% potassium sorbate, 65% polysorbate-80, 3% blue colorant, and the rest is purified water.

[0052] The embodiment of the present invention also discloses a preparation method of the essential oil hoof bath liquid, and the preparation steps include heating, soaking, emulsifying, mixing, dissolving and cooling processes; wherein the essential oil hoof bath liquid preparation process mainly comprises equipment consisting of four main parts: a pure water tank, a mixing soaking tank (reaction tank A), a heating emulsifying mixing tank (reaction tank B), and a cooling mixing tank (reaction tank C), and the four tanks are connected to each other through a water pump.

[0053] The specific preparation process is:

[0054] Put the essential oil, antioxidant and synergist in the formula amount into the reaction tank B, heat it, and stir it appropriately until it is completely dissolved; then put the emulsifier into the first step of emulsification;

[0055] In another reaction tank A, soak the preservative in purified water to dissolve it, and stir it appropriately. When the preservative in reaction tank A is completely dissolved, pump the material into reaction tank B for the second step of emulsification and mixing, maintain a certain temperature, and slowly add the colorant. After emulsification for a certain period of time, stop emulsification, let it stand for 1-2 hours, and if it is qualified in the initial inspection, pump the qualified liquid into reaction tank C for cooling to obtain the essential oil hoof bath.

[0056] In some optional embodiments, the temperature in the reaction tank B should reach above 50°C, generally 70°C-90°C.

[0057] In some optional embodiments, the first emulsification speed is maintained at about 900 rpm. The second emulsification speed is maintained at about 1200 rpm.

[0058] In some optional embodiments, the temperature during the second step of emulsification and mixing is maintained at about 55°C.

[0059] In some optional embodiments, the second step of emulsification and mixing is preferably performed for 15 minutes.

[0060] In some optional embodiments, after the second step of emulsification is completed, it is necessary to stand for 1-2 hours to ensure that the obtained essential oil hoof bath liquid does not produce stratification, that is, it is a qualified product.

[0061] The "room temperature" in the present invention refers to 20-30°C unless otherwise specified.

[0062] The "parts" described in the present invention, unless otherwise specified, refer to parts by mass.

[0063] The raw materials used in the present invention are all purchased from the market. The blue colorant used in the following examples is purchased from Tianjin Shenghui Company, and the product implements the standard GB1886.217.

[0064] The technical solution of the present invention is further illustrated by the following embodiments.

[0065] Example 1

[0066] A method for preparing an essential oil hoof bath comprises the following steps:

[0067] (1) Weigh the following raw materials according to weight percentage:

[0068] Thymol 7%, cinnamaldehyde 15%, tert-butylhydroquinone (TBHQ) + enhancer (citric acid) 0.2% (wherein, by mass ratio, tert-butylhydroquinone: citric acid = 6:4), potassium sorbate 0.2%, polysorbate-80 65%, blue colorant 3%, and the rest is purified water.

[0069] (2) Put the formulated amount of essential oil (thymol, cinnamaldehyde), antioxidant (tert-butylhydroquinone) and synergist (citric acid) into reaction tank B (heating emulsification mixing tank, the temperature in the tank is 70° C.), heat, and stir appropriately until all are dissolved; then add polysorbate-80 and perform the first step of emulsification at a speed of 900 rpm;

[0070] In another reaction tank A (mixing and soaking tank), potassium sorbate is put into purified water for soaking and dissolving, and stirred appropriately at the same time; after the reaction tank A is completely dissolved, the material therein is pumped into the reaction tank B, and the second step of emulsification and mixing is carried out at a temperature of 55°C and a rotation speed of 1200rpm for 15 minutes, the temperature of 55°C is maintained, and a blue colorant is slowly added thereto. After emulsification for 15 minutes, the emulsification is stopped, and the mixture is allowed to stand for 1-2 hours. If the initial inspection is qualified (it is determined that the obtained essential oil hoof bath liquid does not produce stratification), the qualified liquid is pumped into the reaction tank C (cooling mixing tank) for cooling to obtain the essential oil hoof bath liquid.

[0071] Example 2

[0072] A method for preparing an essential oil hoof bath liquid is different from that of Example 1 in that the amounts of raw materials used are different. The specific raw materials are:

[0073] Thymol 7%, cinnamaldehyde 10%, eugenol 5%, tert-butylhydroquinone (TBHQ) + enhancer (citric acid) 0.2% (wherein, by mass ratio, tert-butylhydroquinone: citric acid = 6:4), potassium sorbate 0.2%, polysorbate-80 65%, blue colorant 3%, and the rest is purified water.

[0074] Other preparation steps and conditions are the same as in Example 1.

[0075] Example 3

[0076] A method for preparing an essential oil hoof bath liquid is different from that of Example 1 in that the amounts of raw materials used are different. The specific raw materials are:

[0077] Thymol 5%, cinnamaldehyde 10%, eugenol 3%, carvacrol 4%, tert-butylhydroquinone (TBHQ) + enhancer (citric acid) 0.2% (wherein, by mass ratio, tert-butylhydroquinone: citric acid = 6:4), potassium sorbate 0.2%, polysorbate-80 65%, blue colorant 3%, and the rest is purified water.

[0078] Other preparation steps and conditions are the same as in Example 1.

[0079] Comparative Example 1

[0080] Purchased qualified copper sulfate from a company in the market.

[0081] Effect test

[0082] Using clean water as a blank control, the therapeutic effects of the essential oil hoof bath prepared in Examples 1-3 and the copper sulfate purchased in Comparative Example 1 on hoof diseases of dairy cows were verified.

[0083] (1) The verification test conditions are:

[0084] The essential oil hoof bath liquids prepared in Examples 1-3 were diluted with water to prepare 0.5 wt.% solutions; the copper sulfate in Comparative Example 1 was dissolved in water to prepare a 10 wt.% solution;

[0085] At a cattle farm in Zhaoqing, Guangdong, 50 cattle with obvious hoof diseases were selected and divided into five groups, with 10 in each group. For each cow, the hoof was first cleaned with clean water, and then the necrotic tissue in the diseased part was removed. A special hoof bath was set up for each group, and the height of the hoof bath liquid in the hoof bath was not less than 20 cm. The sick cows were hoof bathed twice a day, morning and evening, and each cow was soaked for 30 minutes each time. A cycle lasted for 5 consecutive days, and three cycles were recorded.

[0086] (2) Determination method:

[0087] ① Selection of cattle with hoof disease: The cattle often lie on the ground, are unwilling to get up when driven, have an unstable standing posture after getting up, have obvious lameness when walking, have pus in the affected area, have increased hoof temperature in the affected limb, and are sensitive to percussion pain, which can be determined as suffering from hoof disease.

[0088] ②Efficacy determination criteria:

[0089] There are obvious effects: the lameness, pain and pus of sick cows are reduced or disappear, and the wound surface begins to heal.

[0090] The effect is not obvious: the sick cows are still lame, painful on percussion, pus oozing, and swollen.

[0091] Analysis of results: After 14 days of hoof bathing, the corresponding treatment effect data on the 5th, 10th and 14th days are shown in Table 1-3.

[0092] Table 1 Data on the 5th day

[0093] Group Number of cases Number of heads with obvious effect The effect is not obvious Cure rate Blank control group 10 0 10 0％ Comparative Example 1 10 0 10 0％ Example 1 10 5 5 50％ Example 2 10 6 4 60％ Example 3 10 5 5 50％

[0094] Table 2 Data on the 10th day

[0095]

[0096]

[0097] Table 3 Data on Day 14

[0098] Group Number of cases Number of effective heads The effect is not obvious Cure rate Blank control group 10 2 8 20％ Comparative Example 1 10 7 3 70％ Example 1 10 9 1 90％ Example 2 10 10 0 100％ Example 3 10 9 1 90％

[0099] It can be seen from the data in Tables 1-3 that Examples 1-3 all show good use effects and have good therapeutic effects on cow hoof diseases, among which Example 2 is particularly excellent, and both the treatment speed and the treatment effect exceed those of other test groups. In the process of verifying the therapeutic effect, the cows that were hoof bathed with Examples 1-3 obviously recovered faster, the pus in the affected area disappeared, and the stimulation to the cows was also small. Although the copper sulfate solution in Comparative Example 1 has a certain therapeutic effect, the recovery speed is slow and it also causes certain pollution to the environment.

[0100] The above are only preferred specific implementations of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. An essential oil hoof bath, characterized in that: The invention comprises essential oil, wherein the essential oil is selected from at least two of thymol, cinnamaldehyde, eugenol or carvacrol; the content of the essential oil in the essential oil hoof bath liquid is 20wt.%-25wt.%.

2. The essential oil hoof bath liquid according to claim 1, characterized in that: The essential oil hoof bath liquid comprises the following raw materials in terms of weight percentage: Essential oil 20%-25%, antioxidant + synergist 0.2%-0.5%, preservative 0.2%-0.5%, emulsifier 60%-75%, colorant 3%-5%, the balance is solvent.

3. The essential oil hoof bath liquid according to claim 2, characterized in that: The antioxidant is selected from at least one of tert-butylhydroquinone, butylated hydroxyanisole or butylated hydroxytoluene; and / or The synergist is selected from at least one of citric acid and vitamin C; and / or The preservative is potassium sorbate; and / or The emulsifier is polysorbate 80; and / or The solvent is purified water.

4. The essential oil hoof bath liquid according to claim 3, characterized in that: The essential oil hoof bath liquid comprises the following raw materials in terms of weight percentage: Thymol 7%, cinnamaldehyde 10%, eugenol 5%, tert-butylhydroquinone + citric acid 0.2%, potassium sorbate 0.2%, polysorbate-806 5%, colorant 3%, and the balance is purified water.

5. A method for preparing an essential oil hoof bath, characterized in that: The following steps are involved: The essential oil, antioxidant and synergist are mixed and heated to make them all melt; then an emulsifier is added thereto for the first step of emulsification to obtain a mixture; The preservative is dissolved in a solvent and then added to the mixture for a second step of emulsification, and then a colorant is added for a third step of emulsification, and then the emulsification is stopped, the mixture is allowed to stand, and cooled to obtain the essential oil hoof bath liquid according to any one of claims 1 to 4.

6. The method for preparing an essential oil hoof bath liquid according to claim 5, characterized in that: The temperature during the heating process is 70°C-90°C.

7. The method for preparing an essential oil hoof bath liquid according to claim 5, characterized in that: The rotation speed during the first emulsification step is 900 rpm.

8. The method for preparing an essential oil hoof bath liquid according to claim 5, characterized in that: The conditions in the second and third emulsification steps are: rotation speed of 1200 rpm, temperature of 55° C., and emulsification time of 15 minutes.

9. The method for preparing an essential oil hoof bath liquid according to claim 5, characterized in that: The standing time is 1-2 hours.

10. Use of the essential oil hoof bath liquid as claimed in any one of claims 1 to 4 in the preparation of medicines or health products for treating hoof diseases of cattle.

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