Antibacterial agent as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of antibacterial agents, and particularly relates to an antibacterial agent and a preparation method and application thereof. The antibacterial agent provided by the invention comprises the following components in parts by weight: 10-30 parts of wikstroemia indica, 20-40 parts of elecampane, 10-30 parts of asparagus cochinchinensis, 20-40 parts of sanguisorba officinalis, 10-30 parts of ligusticum wallichii, 10-30 parts of turmeric, 3-10 parts of radix sophorae flavescentis, 3-10 parts of aloe, 3-10 parts of mint, 3-10 parts of dandelion, 3-7 parts of folium artemisiae argyi, 3-7 parts of radix rubiae, 3-7 parts of pseudo-ginseng, 5-15 parts of rhizoma nardostachyos, 5-15 parts of radix zanthoxyli, 5-15 parts of felwort, 5-15 parts of hemp seeds, 5-15 parts of radix semiaquilegiae and 5-15 parts of elecampane. And 5-15 parts of fructus cnidii. The antibacterial agent prepared from the raw materials with the characteristics is suitable for gynecology, has the effects of diminishing inflammation, resisting bacteria, relieving itching and removing peculiar smell, has the effects of dispelling cold and promoting diuresis on the private parts of women and has a relatively strong inhibiting and killing effect on staphylococcus aureus, escherichia coli and tritirachium album, and the inhibiting and killing effect time is obviously prolonged.

Description

Technical Field

[0001] The invention belongs to the technical field of antibacterial agents, and specifically relates to an antibacterial agent and a preparation method and application thereof. Background Art

[0002] Today's social environmental pollution and fast-paced life have brought multiple pressures from family, society and spiritual levels to women, especially working women, leading to the widespread and frequent occurrence of gynecological diseases in women, which troubles women's lives. Most of the existing gynecological antibacterial agents are made of chemical substances. Long-term use will cause chemical drug residues and side effects, which cannot be metabolized by organs, causing irreversible effects on women's bodies, seriously affecting women's physical and mental health and life. The existing antibacterial agents prepared by traditional Chinese medicine have a short antibacterial time and the antibacterial effect still needs to be improved. Summary of the invention

[0003] Therefore, the present invention aims to solve the defects of the above existing antibacterial agents, thereby providing an antibacterial agent and a preparation method and application thereof.

[0004] The invention provides an antibacterial agent. The antibacterial agent comprises, by weight, 10-30 parts of radix strychnifoliae, 20-40 parts of elecampane, 10-30 parts of asparagus cochinchinensis, 20-40 parts of sanguisorba officinalis, 10-30 parts of ligusticum chuanxiong, 10-30 parts of turmeric, 3-10 parts of sophora flavescens, 3-10 parts of aloe, 3-10 parts of mint, 3-10 parts of dandelion, 3-7 parts of wormwood, 3-7 parts of madder, 3-7 parts of notoginseng, 5-15 parts of nard, 5-15 parts of zelkova chinensis, 5-15 parts of gentian, 5-15 parts of hemp seeds, 5-15 parts of smilax china seeds, 5-15 parts of costusroot and 5-15 parts of cnidium monnieri.

[0005] Sophora flavescens: contains matrine and cytisine; it can clear away heat and dampness. Sophora flavescens extract has antibacterial and anti-inflammatory effects, and can inhibit skin itching.

[0006] Mint: Contains menthol, a terpene organic compound, which is used externally for local analgesia, itching, and itching of the skin. It can be used several times a day. It acts on the skin or mucous membranes and has a cooling and itching effect.

[0007] Dandelion: Contains dandelion alcohol and dandelion. It can clear away heat and detoxify, reduce swelling and resolve knots, promote diuresis and relieve stranguria, and treat urinary tract infection. It is high in potassium, calcium, zinc and manganese, which is beneficial for maintaining the body's acid-base balance.

[0008] Mugwort leaf: contains volatile oil and flavonoid compounds. It is suitable for menorrhagia, bleeding from the fetus, cold pain in the lower abdomen, irregular menstruation, and external treatment of skin itching.

[0009] Rhizoma Leucopteris: contains daphnetin, triticiflavin, etc. It can clear away heat and detoxify, reduce swelling and relieve pain. The extract of Rhizoma Leucopteris has an inhibitory effect on Staphylococcus aureus, hemolytic streptococci, hepatitis B virus, and HIV.

[0010] Chuanxiong: Contains sodium ferulate, chuanxiong lactone, etc. It relieves pain and promotes blood circulation. In vitro tests of antibacterial effects show that the extract of Chuanxiong has an inhibitory effect on large intestine, dysentery (Sonne's), deformity, pus, typhoid, paratyphoid bacillus and Vibrio cholerae. The water extract of Chuanxiong extract also has an inhibitory effect on some pathogenic skin fungi in test tubes.

[0011] Inula: Contains volatile oils, costus acid, etc. In vitro experiments have shown antibacterial effects, and it also has inhibitory effects on Staphylococcus aureus, Shigella dysenteriae and Pseudomonas aeruginosa. It also has inhibitory effects on skin fungi. Contains volatile oils and inulin, dammardienolide acetate, has a killing effect on vaginal droplets, and has a complete inhibitory effect on Mycobacterium tuberculosis, Trichophyton mentagrophytes and Microsporum canis.

[0012] Panax notoginseng: contains saponins, ginsenosides, etc. Raw Panax notoginseng has the effect of treating blood stasis, traumatic bleeding, postpartum blood fainting, and vomiting blood. Cooked Panax notoginseng has the effect of treating physical weakness, loss of appetite, neurasthenia, excessive fatigue, blood loss, anemia, etc.

[0013] Turmeric: Contains volatile oils, curcumin, etc. It can promote blood circulation and remove blood stasis. Curcuma oil can change and enhance the immunogenicity of tumor cells, and also has effects on anti-cancer, anti-thrombosis, liver protection and kidney protection. A plant from which spices are extracted. Contains curcumin, which has anti-tumor effects. It has a strong inhibitory and killing effect on Gram-negative and Gram-positive bacteria (such as Escherichia coli, Staphylococcus aureus) and fungi (Candida albicans).

[0014] Nardostachys grandiflora: Contains galangal, galangal ketone, etc., which can remove dampness and reduce swelling. Its rhizomes have a strong pine oil aroma and a warm and natural fragrance.

[0015] Rubia cordifolia: The water extract of Rubia cordifolia root has a certain inhibitory effect on Staphylococcus aureus, and also has inhibitory effects on Streptococcus pneumoniae, Haemophilus influenzae and some fungi.

[0016] Two-sided needle: The roots and stems of two-sided needle have inhibitory effects on Escherichia coli, Salmonella, Bacillus subtilis, Staphylococcus aureus and Candida albicans.

[0017] Aloe vera: Aloe vera extract has strong antibacterial effects on Staphylococcus aureus, Candida, Aspergillus oryzae and Trichoderma.

[0018] Sanguisorba officinalis: The extract of Sanguisorba officinalis has a good antibacterial effect on Staphylococcus aureus.

[0019] Gentian: Gentian extract has varying degrees of inhibitory effects on Pseudomonas aeruginosa, Proteus, Salmonella typhi, Shigella dysenteriae, Staphylococcus aureus, etc.

[0020] Hemp seeds: Hemp seed extracts have inhibitory effects on Escherichia coli, Staphylococcus aureus and Bacillus subtilis.

[0021] Achyranthes bidentata: The coumarins contained in the extract of Achyranthes bidentata have good antibacterial effect.

[0022] Costus root: The extract of costus root can inhibit the growth of streptococci, golden yellow and white grapes, and has a weak effect on Escherichia coli and diphtheria bacteria.

[0023] Asparagus: Contains asparagine, citrulline, etc. Asparagus extract has different degrees of antibacterial effect on Staphylococcus aureus, Pneumococcus, hemolytic Streptococcus, Corynebacterium diphtheriae, Pseudomonas aeruginosa, and Bacillus anthracis. Antibacterial effect: The decoction in vitro test has different degrees of antibacterial effect on Bacillus anthracis, type A and type B hemolytic Streptococcus, Corynebacterium diphtheriae, diphtheroid Corynebacterium, Pneumococcus, Staphylococcus aureus, Staphylococcus citrinum, Staphylococcus albus, and Bacillus subtilis.

[0024] Cnidium monnieri: Contains osthole, isoborneol, and methoxycarvacrol. Used for impotence, cold uterus, vulvar eczema, vaginal itching in women, and trichomonas vaginitis.

[0025] Preferably, the antibacterial agent, by weight, comprises the following raw materials: 20 parts of Rhizoma Dioscoreae, 25 parts of Rhizoma Inulae, 20 parts of Rhizoma Asparagi, 25 parts of Rhizoma Sanguisorbae, 30 parts of Rhizoma Chuanxiong, 20 parts of Rhizoma Curcumae, 10 parts of Rhizoma Sophorae Flavescentis, 10 parts of Aloe Vera, 10 parts of Menthus Fructus, 10 parts of Taraxaci, 5 parts of Folium Artemisiae Argyi, 5 parts of Rubia cordifolia, 5 parts of Notoginseng, 10 parts of Nardostachys grandiflora, 10 parts of Zanthoxylum bungeanum, 10 parts of Gentiana scabra, 10 parts of Hemp Seed, 10 parts of Gastrodia elatior, 10 parts of Aucklandia lappa, and 10 parts of Cnidium monnieri.

[0026] The present invention provides a method for preparing an antibacterial agent, comprising the following steps: 1) Material selection: Select all raw materials that are clean and have a moisture content below 7%; 2) Soaking: adding all the raw materials to 500-1200 parts by weight of water, soaking at 10-45°C for 1.5-2.5 hours to obtain a raw material solution; 3) Boiling: heating the raw material liquid obtained in step 2) to 90-100° C. and then keeping the temperature for 2-3 hours to obtain a boiled liquid; 4) Distillation: distilling the boiled liquid obtained in step 3) to obtain a puree; 5) Preparation of starter: 3-7 parts by weight of pea flour, 1-3 parts by weight of soybean flour, 1-3 parts by weight of corn flour and 2-4 parts by weight of buckwheat flour are taken as culture medium, 1 / 80-3 / 80 parts by weight of the distilled puree is added, and fermented at a temperature of 50-60 degrees for 22-30 days. The white hairy substance formed on the culture medium is the starter; 6) Raw material fermentation: 2-8 parts by weight of water, 0.2-1 parts by weight of lactic acid bacteria and 0.2-1 parts by weight of the starter are mixed evenly to prepare a fermentation mixture, and then the fermentation mixture, the remaining raw pulp and 700-900 parts by weight of water are put into a stirring tank and stirred evenly, and fermented at a temperature of 20-50 degrees for 5-30 to obtain the antibacterial agent.

[0027] Optionally, the solid content of the raw pulp obtained after distillation in step 4) is 40%-80%.

[0028] The solid content of the present invention refers to the mass percentage of the remaining material after the original pulp is dried to the total amount of the original pulp.

[0029] Preferably, in step 2), the mixture is immersed for 2.0 hours; And / or, fermenting for 24 hours in step 6).

[0030] Preferably, the fermentation in step 5) is stopped until the fermented culture medium is no longer sticky, lumpy and loose.

[0031] Preferably, the fermentation time in step 5) is 26 days; The culture medium in step 5) is 5 parts by weight of pea flour, 2 parts by weight of soybean flour, 2 parts by weight of corn flour and 3 parts by weight of buckwheat flour.

[0032] Preferably, the viable count of the lactobacillus in step 6) is 1.5×10 11 -5×10 11 CFU / g; And / or, the lactobacillus is selected from at least one of Lactobacillus crispatus, Lactobacillus acidophilus and Lactobacillus bulgaricus; Preferably, the lactobacilli are Lactobacillus crispatus and Lactobacillus acidophilus; the mass ratio of Lactobacillus crispatus to Lactobacillus acidophilus is (1-5):(1-5).

[0033] The present invention provides an application of the above-mentioned antibacterial agent or the antibacterial agent prepared by the above-mentioned preparation method in gynecological antibacterial treatment.

[0034] The present invention provides a gynecological antibacterial wet wipe, which comprises a non-woven fabric and the antibacterial agent described above or the antibacterial agent prepared by the preparation method described above.

[0035] Preferably, the gynecological antibacterial wet wipes are obtained by soaking non-woven fabric with the antibacterial agent; Optionally, before the step of soaking the non-woven fabric with the antibacterial agent, the step of diluting the antibacterial agent with water by 10-300 times is also included; Optionally, before the step of soaking the non-woven fabric with the antibacterial agent, the step also includes diluting the antibacterial agent with water 100 times.

[0036] The technical solution of the present invention has the following advantages: The antibacterial agent provided by the invention comprises, by weight, 10-30 parts of radix strychnifoliae, 20-40 parts of elecampane, 10-30 parts of asparagus cochinchinensis, 20-40 parts of sanguisorba officinalis, 10-30 parts of ligusticum chuanxiong, 10-30 parts of turmeric, 3-10 parts of sophora flavescens, 3-10 parts of aloe, 3-10 parts of mint, 3-10 parts of dandelion, 3-7 parts of wormwood, 3-7 parts of madder, 3-7 parts of notoginseng, 5-15 parts of nard, 5-15 parts of zelkova chinensis, 5-15 parts of gentian, 5-15 parts of hemp seeds, 5-15 parts of kelp seeds, 5-15 parts of costusroot, and 5-15 parts of cnidium monnieri. The antibacterial agent prepared by using the raw materials with the above characteristics of the present invention is suitable for gynecology, has the effects of anti-inflammatory, antibacterial, antipruritic and deodorizing, and has a strong inhibitory and killing effect on the female private parts, Staphylococcus aureus, Escherichia coli and Candida albicans, and the inhibitory and killing time is significantly prolonged.

[0037] The preparation method of the antibacterial agent provided by the present invention comprises the following steps: 1) material selection: selecting all clean raw materials with a water content of less than 7%; 2) soaking: adding 500-1200 parts by weight of water to all the raw materials, soaking at 10-45°C for 1.5-2.5 hours to obtain a raw material liquid; 3) boiling: heating the raw material liquid obtained in step 2) to 90-100°C, and then keeping the temperature for 2 hours to obtain a boiled liquid; 4) distillation: distilling the boiled liquid obtained in step 3) to obtain a puree for standby use; 5) preparation of a starter: taking 3-7 parts by weight of pea flour, 1-3 parts by weight of soybean flour, and 1- 3 parts by weight of wheat flour and 2-4 parts by weight of buckwheat noodles are used as culture medium, 1 / 80-3 / 80 of the raw pulp after distillation is added, and fermented at a temperature of 50-60 degrees for 22-30 days. The white hairs formed on the culture medium are taken as the starter; 6) Raw material fermentation: 2-8 parts by weight of pure water, 0.2-1 parts by weight of lactic acid bacteria and 0.2-1 parts by weight of the starter are mixed evenly to prepare a fermentation mixture, and then the fermentation mixture, the remaining raw pulp and 700-900 parts by weight of pure water are put into a stirring tank together, stirred evenly, and fermented at a temperature of 20-50 degrees for 5-30 hours to obtain the antibacterial agent. Lactobacillus is mixed with a specific prepared fermentation agent, and is used as a fermentation agent together. Fermentation is carried out in the original pulp obtained by the specific Chinese medicinal material components of the present application, and the drug resistance of lactobacillus in the bactericide can be significantly improved, and the survival rate of lactobacillus is improved. The antibacterial active components produced by the synergistic original pulp are jointly improved to improve the antibacterial effect, and a long-term inhibitory effect is achieved, especially in gynecology. The antibacterial effect is obvious and the antibacterial time is longer. The present invention is through a specific fermentation method, and the antibacterial agent obtained is suitable for gynecology, has anti-inflammatory, antibacterial, antipruritic, and the effect of removing peculiar smell, and has a strong inhibitory and killing effect on the female private parts to dispel cold and dampness and Staphylococcus aureus, Escherichia coli, and Candida albicans, and the inhibitory and killing time is significantly extended.

[0038] The present invention provides a method for preparing an antibacterial agent, wherein the lactobacillus is selected from at least one of Lactobacillus crispatus, Lactobacillus acidophilus and Lactobacillus bulgaricus; when the lactobacillus is preferably Lactobacillus crispatus and Lactobacillus acidophilus; and when the mass ratio of Lactobacillus crispatus and Lactobacillus acidophilus is (1-5):(1-5), the survival rates of Lactobacillus crispatus and Lactobacillus acidophilus in the specific fermentation environment provided by the present invention are higher, the antibacterial effect of the obtained antibacterial agent is more obvious and the antibacterial time is further prolonged.

[0039] The antibacterial agent provided by the present invention or the antibacterial agent prepared by the preparation method provided is used in gynecological antibacterial, is suitable for the development of women's physical and mental health, and has the functions of sterilization, deodorization and maintenance. Monitoring by the Ningbo Customs Technology Center has proved that it has a strong inhibitory and killing effect on other Gram-positive bacteria (such as Staphylococcus aureus), Gram-negative bacteria (such as Escherichia coli, Salmonella) and fungi (such as Candida albicans) except lactobacilli, and the sterilization effect lasts for a long time. DETAILED DESCRIPTION

[0040] The following examples are provided for a better understanding of the present invention, but are not intended to limit the best mode of implementation, nor to limit the content and protection scope of the present invention. Any product identical or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior arts shall fall within the protection scope of the present invention.

[0041] If no specific experimental steps or conditions are specified in the examples, the conventional experimental steps or conditions described in the literature in the field can be used. If no manufacturer is specified for the reagents or instruments used, they are all conventional reagent products that can be obtained commercially.

[0042] Example 1: This example provides an antibacterial agent made from the following raw materials: 20g of Rhizoma Dioscoreae, 25g of Rhizoma Inulae, 20g of Rhizoma Asparagi, 25g of Rhizoma Sanguisorbae, 30g of Rhizoma Chuanxiong, 20g of Curcuma Longa, 10g of Sophora flavescens, 10g of Aloe, 10g of Menthus, 10g of Taraxacum, 5g of Artemisia argyi, 5g of Rubia cordifolia, 5g of Notoginseng, 10g of Nardostachys grandiflora, 10g of Zanthoxylum bungeanum, 10g of Gentiana scabra, 10g of Hemp Seed, 10g of Gastrodia elata, 10g of Aucklandia lappa, and 10g of Cnidium monnieri.

[0043] This embodiment provides a method for preparing an antibacterial agent, comprising the following steps: 1) Material selection: Select all raw materials that are clean and have a moisture content below 7%; 2) Soaking: adding all the raw materials into 1000 g of water to soak to obtain a raw material solution, the soaking temperature is room temperature, and the soaking time is 2.0 hours; 3) Boiling: heating the soaked raw material liquid, boiling the raw material liquid and keeping it warm for 2 hours; 4) Distillation: distilling the boiled raw material liquid, and retaining the original syrup with a solid content of 50% for standby use; 5) Preparation of starter: 5 g of pea flour, 2 g of soybean flour, 2 g of corn flour, and 3 g of buckwheat flour are taken as culture medium, 1 / 40 mass portion of the distilled puree is added, and fermentation is performed at a temperature of 50-60 degrees for 26 days until the fermented culture medium is not sticky, lumpy, or loose, and the white hairy substance on the culture medium is taken as a starter for later use; 6) Raw material fermentation: 5 g of pure water and 0.5 g of the fermentation agent are mixed evenly to prepare a fermentation mixture, and then the fermentation mixture, the remaining raw pulp and 700 g of pure water are put into a stirring tank and stirred evenly, and the mixture is sealed and fermented at a temperature of 37 degrees for 24 hours to obtain the antibacterial agent.

[0044] Example 2: This example provides an antibacterial agent made from the following raw materials: 20g of Rhizoma Dioscoreae, 25g of Rhizoma Inulae, 20g of Rhizoma Asparagi, 25g of Rhizoma Sanguisorbae, 30g of Rhizoma Chuanxiong, 20g of Curcuma Longa, 10g of Sophora flavescens, 10g of Aloe, 10g of Menthus, 10g of Taraxacum, 5g of Artemisia argyi, 5g of Rubia cordifolia, 5g of Notoginseng, 10g of Nardostachys grandiflora, 10g of Zanthoxylum bungeanum, 10g of Gentiana scabra, 10g of Hemp Seed, 10g of Gastrodia elata, 10g of Aucklandia lappa, and 10g of Cnidium monnieri.

[0045] This embodiment provides a method for preparing an antibacterial agent, comprising the following steps: 1) Material selection: Select all raw materials that are clean and have a moisture content below 7%; 2) Soaking: adding all the raw materials into 1000 g of water to soak to obtain a raw material solution, the soaking temperature is room temperature, and the soaking time is 2.0 hours; 3) Boiling: heating the soaked raw material liquid, boiling the raw material liquid and keeping it warm for 2 hours; 4) Distillation: distilling the boiled raw material liquid, and retaining the original syrup with a solid content of 50% for standby use; 5) Preparation of starter: 5 g of pea flour, 2 g of soybean flour, 2 g of corn flour, and 3 g of buckwheat flour are taken as culture medium, 1 / 40 mass portion of the distilled puree is added, and fermentation is performed at a temperature of 50-60 degrees for 26 days until the fermented culture medium is not sticky, lumpy, or loose, and the white hairy substance on the culture medium is taken as a starter for later use; 6) Raw material fermentation: Take 5g of pure water, 0.3g of Lactobacillus bulgaricus (the number of viable bacteria is 4.1×10 11 CFU / g), 0.3g Lactobacillus acidophilus (viable count 4.1×10 11 CFU / g) and 0.5g of the fermentation agent are mixed evenly to form a fermentation mixture, and then the fermentation mixture, the remaining raw pulp and 700g of pure water are put into a stirring tank and stirred evenly, and the mixture is sealed and fermented at a temperature of 37 degrees for 24 hours to obtain the antibacterial agent.

[0046] Example 3: This example provides an antibacterial agent made from the following raw materials: 20g of Rhizoma Dioscoreae, 25g of Rhizoma Inulae, 20g of Rhizoma Asparagi, 25g of Rhizoma Sanguisorbae, 30g of Rhizoma Chuanxiong, 20g of Curcuma Longa, 10g of Sophora flavescens, 10g of Aloe, 10g of Menthus, 10g of Taraxacum, 5g of Artemisia argyi, 5g of Rubia cordifolia, 5g of Notoginseng, 10g of Nardostachys grandiflora, 10g of Zanthoxylum bungeanum, 10g of Gentiana scabra, 10g of Hemp Seed, 10g of Gastrodia elata, 10g of Aucklandia lappa, and 10g of Cnidium monnieri.

[0047] This embodiment provides a method for preparing an antibacterial agent, comprising the following steps: 1) Material selection: Select all raw materials that are clean and have a moisture content below 7%; 2) Soaking: adding all the raw materials into 1000 g of water to soak to obtain a raw material solution, the soaking temperature is room temperature, and the soaking time is 2.0 hours; 3) Boiling: heating the soaked raw material liquid, boiling the raw material liquid and keeping it warm for 2 hours; 4) Distillation: distilling the boiled raw material liquid, and retaining the original syrup with a solid content of 50% for standby use; 5) Raw material fermentation: Take 5g of pure water, 0.3g of Lactobacillus bulgaricus (the number of viable bacteria is 4.1×10 11 CFU / g), 0.3g Lactobacillus acidophilus (viable count 4.1×10 11 CFU / g) were mixed evenly to form a fermentation mixture, and then the fermentation mixture, the original puree and 700g of purified water were put into a stirring tank and stirred evenly, and the mixture was sealed and fermented at a temperature of 37 degrees for 24 hours to obtain the antibacterial agent.

[0048] Example 4: The example provides an antibacterial agent made from the following raw materials: 20g of Rhizoma Dioscoreae, 25g of Rhizoma Inulae, 20g of Rhizoma Asparagi, 25g of Radix Sanguisorbae, 30g of Rhizoma Chuanxiong, 20g of Curcuma Longa, 10g of Sophora flavescens, 10g of Aloe, 10g of Menthus mint, 10g of Taraxacum, 5g of Artemisia argyi, 5g of Rubia cordifolia, 5g of Panax notoginseng, 10g of Nardostachys grandiflora, 10g of Zanthoxylum bungeanum, 10g of Gentiana scabra, 10g of Hemp seed, 10g of Gastrodia elata, 10g of Costus root, and 10g of Cnidium monnieri.

[0049] This embodiment provides a method for preparing an antibacterial agent, comprising the following steps: 1) Material selection: Select all raw materials that are clean and have a moisture content below 7%; 2) Soaking: adding all the raw materials into 1000 g of water to soak to obtain a raw material solution, the soaking temperature is room temperature, and the soaking time is 2.0 hours; 3) Boiling: heating the soaked raw material liquid, boiling the raw material liquid and keeping it warm for 2 hours; 4) Distillation: distilling the boiled raw material liquid, and retaining the original syrup with a solid content of 50% for standby use; 5) Preparation of starter: 5 g of pea flour, 2 g of soybean flour, 2 g of corn flour, and 3 g of buckwheat flour are taken as culture medium, 1 / 40 mass portion of the distilled puree is added, and fermentation is performed at a temperature of 50-60 degrees for 26 days until the fermented culture medium is not sticky, lumpy, or loose, and the white hairy substance on the culture medium is taken as a starter for later use; 6) Raw material fermentation: Take 5g of pure water, 0.3g of Lactobacillus crispatus (the number of viable bacteria is 4.1×10 11 CFU / g), 0.3g Lactobacillus acidophilus (viable count 4.1×10 11 CFU / g) and 0.5g of the fermentation agent are mixed evenly to form a fermentation mixture, and then the fermentation mixture, the remaining raw pulp and 700g of pure water are put into a stirring tank and stirred evenly, and the mixture is sealed and fermented at a temperature of 37 degrees for 24 hours to obtain the antibacterial agent.

[0050] Example 5: This example provides an antibacterial agent made from the following raw materials: 30g of Rhizoma Dioscoreae, 30g of Rhizoma Inulae, 25g of Rhizoma Asparagi, 10g of Radix Sanguisorbae, 15g of Rhizoma Chuanxiong, 25g of Curcuma Longa, 5g of Sophora Flavescentis, 5g of Aloe Vera, 5g of Mentha, 9g of Taraxacum, 7g of Artemisia annua, 7g of Rubia cordifolia, 7g of Notoginseng, 15g of Nardostachys grandiflora, 15g of Zanthoxylum bungeanum, 5g of Gentiana scabra, 15g of Hemp Seed, 15g of Gastrodia elata, 5g of Costusroot, and 15g of Cnidium monnieri.

[0051] This embodiment provides a method for preparing an antibacterial agent, comprising the following steps: 1) Material selection: Select all raw materials that are clean and have a moisture content below 7%; 2) Soaking: adding all the raw materials into 700 g of water to soak to obtain a raw material solution, the soaking temperature is room temperature, and the soaking time is 2.0 hours; 3) Boiling: heating the soaked raw material liquid, boiling the raw material liquid and keeping it warm for 2 hours; 4) Distillation: distilling the boiled raw material liquid, and retaining the original syrup with a solid content of 50% for standby use; 5) Preparation of starter: 5 g of pea flour, 2 g of soybean flour, 2 g of corn flour, and 3 g of buckwheat flour are taken as culture medium, 1 / 40 mass portion of the distilled puree is added, and fermentation is performed at a temperature of 50-60 degrees for 26 days until the fermented culture medium is not sticky, lumpy, or loose, and the white hairy substance on the culture medium is taken as a starter for later use; 6) Raw material fermentation: Take 5g of pure water, 0.3g of Lactobacillus crispatus (the number of viable bacteria is 4.1×10 11CFU / g), 0.3g Lactobacillus acidophilus (viable count 4.1×10 11 CFU / g) and 0.5g of the fermentation agent are mixed evenly to form a fermentation mixture, and then the fermentation mixture, the remaining raw pulp and 700g of pure water are put into a stirring tank and stirred evenly, and the mixture is sealed and fermented at a temperature of 37 degrees for 24 hours to obtain the antibacterial agent.

[0052] Example 6: This example provides a gynecological antibacterial wet wipe, which is obtained by diluting the antibacterial agent obtained in Example 1 by 100 times and then soaking the non-woven fabric.

[0053] Example 7: This example provides a gynecological antibacterial wet wipe, which is obtained by diluting the antibacterial agent obtained in Example 2 by 100 times and then soaking the non-woven fabric.

[0054] Example 8: This example provides a gynecological antibacterial wet wipe, which is obtained by diluting the antibacterial agent obtained in Example 3 by 100 times and then soaking the non-woven fabric.

[0055] Example 9: This example provides a gynecological antibacterial wet wipe, which is obtained by diluting the antibacterial agent obtained in Example 4 by 100 times and then soaking the non-woven fabric.

[0056] Example 10: This example provides a gynecological antibacterial wet wipe, which is obtained by diluting the antibacterial agent obtained in Example 5 by 100 times and then soaking the non-woven fabric.

[0057] Comparative Example 1: This comparative example provides an antibacterial agent, which differs from Example 1 only in that the raw material is 265g of the following raw materials, calculated by weight: 20 parts of asparagus, 25 parts of sanguisorba officinalis, 10 parts of aloe, 5 parts of madder, 10 parts of zelkova, 10 parts of gentian, 10 parts of hemp seeds, 10 parts of amaranth seeds, and 10 parts of costus root.

[0058] The method for preparing the antibacterial agent provided in this comparative example is different from that in Example 1 only in that the raw materials are the raw materials described above.

[0059] Comparative Example 2: This comparative example provides an antibacterial agent, which differs from Example 1 only in that the raw material is 265g of the following raw materials, calculated by weight: 30 parts of golden pine, 15 parts of Angelica dahurica, 5 parts of Saposhnikovia divaricata, 5 parts of Panax notoginseng, 20 parts of Asparagus cochinchinensis, 25 parts of Sanguisorba officinalis, 10 parts of Aloe vera, 5 parts of Rubia cordifolia, 10 parts of Zanthoxylum bungeanum, 10 parts of Gentiana scabra, 10 parts of Hemp seeds, 10 parts of Gastrodia elata seeds, and 10 parts of Costusroot.

[0060] The method for preparing the antibacterial agent provided in this comparative example is different from that in Example 1 only in that the raw materials are the raw materials described above.

[0061] Test Case After diluting the antibacterial agents prepared in Examples 1-5 and Comparative Examples 1-2 by 100 times, an equal amount of the antibacterial agent dilution was taken and tested for antibacterial properties against Escherichia coli ATCC8099, Staphylococcus aureus ATCC6538, and Candida albicans ATCC10231 according to the method specified in Appendix C of the "Single-use Hygiene Standard GB15979-2002". The action times were 2 min, 5 min, 10 min, and 20 min, respectively. The experiment was repeated 3 times for each example, and the average sterilization rate was taken. The test results are shown in Table 1. Then the cells were taken out of the sterile environment and placed in an environment contaminated with Escherichia coli, Staphylococcus aureus and Candida albicans for continuous culture. The total colony counts of Escherichia coli, Staphylococcus aureus and Candida albicans were detected on 0.5d, 1d, 1.5d, 2, 2.5d, 3d, 3.5d, 4d, 4.5d, 5d, 5.6d and 6d, respectively. When the total colony counts of Escherichia coli, Staphylococcus aureus and Candida albicans began to increase significantly (more than 2 times) compared with the total colony counts at 0.5d, it indicated that the bactericidal effect of the antimicrobial agent weakened, and the time point of the significant increase was recorded. The results are shown in Table 1.

[0062] Table 1

[0063] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. An antibacterial agent, characterized in that The antibacterial agent comprises, by weight, 10-30 parts of Radix Angelicae Pubescentis, 20-40 parts of Rhizoma Inulae, 10-30 parts of Rhizoma Asparagi, 20-40 parts of Radix Sanguisorbae, 10-30 parts of Rhizoma Chuanxiong, 10-30 parts of Rhizoma Curcumae, 3-10 parts of Sophora flavescens, 3-10 parts of Aloe, 3-10 parts of Menthus mint, 3-10 parts of Taraxacum, 3-7 parts of Folium Artemisiae Argyi, 3-7 parts of Rubia cordifolia, 3-7 parts of Notoginseng, 5-15 parts of Nardostachys grandiflora, 5-15 parts of Zanthoxylum bungeanum, 5-15 parts of Gentiana scabra, 5-15 parts of Semen Hempseeds, 5-15 parts of Gastrodia elata, 5-15 parts of Aucklandia lappa and 5-15 parts of Cnidium monnieri.

2. The antibacterial agent according to claim 1, characterized in that The antibacterial agent comprises, by weight, 20 parts of Radix Angelicae Pubescentis, 25 parts of Rhizoma Inulae, 20 parts of Rhizoma Asparagi, 25 parts of Radix Sanguisorbae, 30 parts of Rhizoma Chuanxiong, 20 parts of Rhizoma Curcumae, 10 parts of Sophora flavescens, 10 parts of Aloe, 10 parts of Menthus mint, 10 parts of Taraxaci, 5 parts of Folium Artemisiae Argyi, 5 parts of Rubia cordifolia, 5 parts of Notoginseng, 10 parts of Nardostachys grandiflora, 10 parts of Zanthoxylum bungeanum, 10 parts of Gentiana scabra, 10 parts of Semen Hemp, 10 parts of Fructus Achyranthis Bidentatae, 10 parts of Fructus Aucklandiae, and 10 parts of Fructus Cnidium.

3. A method for preparing an antibacterial agent, characterized in that: The following steps are involved: 1) Material selection: Select all raw materials that are clean and have a moisture content below 7%; 2) Soaking: adding all the raw materials to 500-1200 parts by weight of water, soaking at 10-45°C for 1.5-2.5 hours to obtain a raw material solution; 3) Boiling: heating the raw material liquid obtained in step 2) to 90-100° C. and then keeping the temperature for 2-3 hours to obtain a boiled liquid; 4) Distillation: distilling the boiled liquid obtained in step 3) to obtain a puree for later use; 5) Preparation of starter: 3-7 parts by weight of pea flour, 1-3 parts by weight of soybean flour, 1-3 parts by weight of corn flour and 2-4 parts by weight of buckwheat flour are used as culture medium, 1 / 80-3 / 80 of the distilled puree is added, and fermented at a temperature of 50-60 degrees for 22-30 days. The white hairy substance formed on the culture medium is the starter; 6) Raw material fermentation: 2-8 parts by weight of water, 0.2-1 parts by weight of lactic acid bacteria and 0.2-1 parts by weight of the starter are mixed evenly to prepare a fermentation mixture, and then the fermentation mixture, the remaining raw pulp and 700-900 parts by weight of water are put into a stirring tank and stirred evenly, and fermented at a temperature of 20-50 degrees for 5-30 hours to obtain the antibacterial agent.

4. The method for preparing the antibacterial agent according to claim 3, characterized in that: Step 2) soaking for 2.0 hours; And / or, fermenting for 24 hours in step 6).

5. The method for preparing the antibacterial agent according to claim 3 or 4, characterized in that: The fermentation in step 5) is stopped until the fermented medium is no longer sticky, lumpy and loose.

6. The method for preparing the antibacterial agent according to any one of claims 3 to 5, characterized in that: The fermentation time in step 5) is 26 days; The culture medium in step 5) is 5 parts by weight of pea flour, 2 parts by weight of soybean flour, 2 parts by weight of corn flour and 3 parts by weight of buckwheat flour.

7. The method for preparing the antibacterial agent according to any one of claims 3 to 6, characterized in that: The viable count of lactobacillus in step 6) is 1.5×10 11 -5×10 11 CFU / g; And / or, the lactobacillus is selected from at least one of Lactobacillus crispatus, Lactobacillus acidophilus and Lactobacillus bulgaricus; Preferably, the lactobacilli are Lactobacillus crispatus and Lactobacillus acidophilus; the mass ratio of Lactobacillus crispatus to Lactobacillus acidophilus is (1-5):(1-5).

8. Use of the antibacterial agent according to claim 1 or 2 or the antibacterial agent prepared by the preparation method according to any one of claims 3 to 7 in gynecological antibacterial treatment.

9. A gynecological antibacterial wet wipe, characterized in that: The gynecological antibacterial wet wipes include non-woven fabrics and the antibacterial agent according to claim 1 or 2 or the antibacterial agent prepared by the preparation method according to any one of claims 3 to 7.

10. The gynecological antibacterial wet wipes according to claim 9, characterized in that: The gynecological antibacterial wet wipes are obtained by soaking non-woven fabric with the antibacterial agent; Optionally, before the step of soaking the non-woven fabric with the antibacterial agent, the step of diluting the antibacterial agent with water by 10-300 times is also included; Optionally, before the step of soaking the non-woven fabric with the antibacterial agent, the step also includes diluting the antibacterial agent with water 100 times.

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