Application of royal jelly protein in Parkinson medicine and preparation equipment of royal jelly protein

Abstract

The invention discloses application of royal jelly protein in a Parkinson's disease drug and preparation equipment thereof, and the key point of the technical scheme is that the application of the royal jelly protein in the Parkinson's disease drug comprises the following steps: step 1, extraction of royal jelly major protein: crushing, filtering, centrifuging, precipitating and purifying the obtained royal jelly to obtain the purified royal jelly major protein; step 2, preparing raw materials, namely preparing the following raw materials in parts by weight: 1.5 to 2.0 parts of uncaria, 1.8 to 3.2 parts of gastrodia elata, 2.2 to 3.6 parts of tribulus terrestris, 0.8 to 1.2 parts of concha haliotidis, 4.2 to 5.0 parts of prepared rehmannia root, 0.4 to 1.6 parts of scorpion and 3.2 to 4.8 parts of royal jelly major protein; the royal jelly main protein plays a role of an accelerant to activate neural stem cells of a mature brain, so that the neural stem cells are expected to be differentiated into neurons and glial cells, and the royal jelly main protein can improve the drug effect bearing effect of a patient and further improve the treatment effect while improving the Parkinson drug treatment effect.

Description

Technical Field

[0001] The invention relates to the technical field of royal jelly proteins, and in particular to the application of royal jelly proteins in Parkinson's drugs and a preparation device thereof. Background Art

[0002] Parkinson's disease (PD) is a common neurodegenerative disease that is more common in the elderly, with an average age of onset of about 60 years old. It is rare for young people to develop Parkinson's disease before the age of 40. The main pathological change of Parkinson's disease is the degeneration and death of dopamine (DA) neurons in the substantia nigra of the midbrain, which causes a significant decrease in the DA content in the striatum and causes the disease. The exact cause of this pathological change is still unclear. Genetic factors, environmental factors, aging, oxidative stress, etc. may all be involved in the degeneration and death of dopamine neurons in PD.

[0003] The protein in royal jelly accounts for about 50% of the dry matter, of which 2 / 3 is albumin and 1 / 3 is globulin, which is roughly similar to the ratio of albumin to globulin in human blood. The amino acids in royal jelly account for 0.8% of the dry weight, about 549.105mg / 100g, which is more than 70 times that of honey, and the amino acid variety is rich (the qualitative analysis results of the Department of Biology of Sun Yat-sen University on my country's royal jelly show that it contains 18 kinds of amino acids), of which the essential amino acids are about 241.987mg / g.

[0004] When currently used, Parkinson's drugs can cause long-term symptom fluctuations and adverse reactions such as dyskinesia, which weaken the body and make it impossible for patients to tolerate the effects of the drugs, further reducing the effectiveness of treatment. Summary of the invention

[0005] The purpose of the present invention is to provide the application of royal jelly protein in Parkinson's disease drugs and its preparation equipment to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] The application of royal jelly protein in Parkinson's disease drugs comprises the following steps:

[0008] Step 1: Extraction of royal jelly protein: crushing, filtering, centrifuging, precipitating and purifying the obtained royal jelly to obtain purified royal jelly protein;

[0009] Step 2, prepare raw materials according to the following raw material composition in parts by weight: 1.5-2.0 parts of Uncaria rhynchophylla, 1.8-3.2 parts of Gastrodia elata, 2.2-3.6 parts of Tribulus terrestris, 0.8-1.2 parts of Semen Cassiae, 4.2-5.0 parts of Rehmannia glutinosa, 0.4-1.6 parts of Scorpio and 3.2-4.8 parts of royal jelly protein;

[0010] Step 3, obtaining a medicinal solution, mixing and grinding the prepared Uncaria rhynchophylla, Gastrodia elata, Tribulus terrestris, and Semen Cassiae, grinding Rehmannia glutinosa and Scorpio glutinosa separately, and sieving them all through a 100-120 mesh sieve, boiling the sieved mixed powder in water for 1.5-2.5 hours, adding Rehmannia glutinosa and Scorpio glutinosa powder, and continuing to boil in water for 1.0-1.5 hours, filtering the medicinal residues to obtain a medicinal solution;

[0011] Step 4: Process the drug preparation, concentrate the drug solution into an extract, add the royal jelly main protein into the extract, stir, and vacuum dry to obtain a dry paste, crush the dry paste, and add pharmaceutically acceptable excipients to prepare a drug preparation commonly used in pharmacy.

[0012] Preferably, in step one, when the centrifugal treatment is performed, the centrifugal equipment is kept running in a low-temperature environment. In the purification treatment, the royal jelly protein is purified by ion exchange chromatography. The mutual charge interaction between the royal jelly protein and the ion exchange resin purifies and concentrates the royal jelly protein.

[0013] Preferably, the centrifugal speed of the centrifugal device is controlled at 12000 rpm-3600 rpm, and the centrifugal treatment is maintained at a temperature of 1-3°C.

[0014] Preferably, the pharmaceutically acceptable excipients include starch, dextrin, magnesium stearate and microcrystalline cellulose.

[0015] Preferably, the preparation is one of powder, decoction, granules, tablets, capsules, oral liquid, pills or powder.

[0016] The preparation equipment of royal jelly protein in Parkinson's disease drugs includes a conical crushing box, a filter box, a transmission component, a crushing disc, a rubber grinding scraper, a filter tube, a push rod component, an end cover and a filtrate sheet. The conical crushing box is fixed on the filter box and fixedly connected to the transmission component. The crushing disc and the rubber grinding scraper are both fixed on the lower end of the transmission component. The rubber grinding scraper is in contact with the bottom of the conical crushing box. The filter tube is fixed in the filter box and connected to the lower end of the conical crushing box. The push rod component is installed on the filter tube. The end cover is threadedly connected to the bottom of the filter tube and is engaged with the filtrate sheet.

[0017] Preferably, the transmission assembly includes a servo motor, a transmission shaft and a cross support frame, the cross support frame is fixed on the conical crushing box and is rotatably connected to the transmission shaft through a bearing, the crushing blade and the rubber grinding scraper are both fixed on the transmission shaft, the servo motor is fixed on the conical crushing box, and the motor shaft is transmission-connected to the transmission shaft through a coupling.

[0018] Preferably, a plurality of equally spaced flow holes are provided at the lower end of the conical crushing box.

[0019] Preferably, the push rod assembly includes an electric push rod and a sealing piston plate, the electric push rod is fixed on the filter tube, and the output end is fixedly connected to the sealing piston plate, and the sealing piston plate is sleeved in the filter tube.

[0020] Preferably, a material injection port is provided above the conical crushing box, and a sealing cover is threadedly connected to the material injection port.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] In the application of the royal jelly protein in Parkinson's disease drugs, the superoxide dismutase in the royal jelly protein is the main antioxidant component, which has a better nutritional supplement effect on people with malnutrition, weakness after illness and physical exhaustion in the elderly. It can also stimulate the production of glial cell-derived neurotrophic factor (GDNF) to play a neurotrophic effect on the mature brain. The enhanced expression of neurofilament HmRNA involves the subsequent neurotrophic factor GDNF. The royal jelly protein plays a neurotrophic or neuroprotective role in the mature brain through GDNF. The royal jelly protein makes the differentiation of all different types of brain cells easier, including neurons, astrocytes and oligodendrocytes. It also improves the function of neurons by promoting the regeneration of hippocampal granule cells (function in the cognitive process). The royal jelly protein plays the role of a promoter to activate the neural stem cells of the mature brain, thereby expecting it to differentiate into neurons and glial cells. While improving the effect of Parkinson's disease drug treatment, the royal jelly protein can also improve the patient's tolerance to the drug effect, thereby further improving the treatment effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a structural flow chart of the present invention;

[0024] Figure 2 It is a cross-sectional view of the structure of the preparation equipment of the present invention;

[0025] Figure 3 It is a structural diagram of the present invention.

[0026] In the figure: 1. Conical crushing box; 2. Filter box; 3. Transmission assembly; 31. Servo motor; 32. Transmission shaft; 33. Cross support frame; 4. Crushing blade; 5. Rubber grinding scraper; 6. Filter tube; 7. End cover; 8. Filtrate sheet; 9. Push rod assembly; 91. Electric push rod; 92. Sealing piston plate; 10. Flow hole; 11. Injection port; 12. Sealing cover. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Example 1

[0029] See also Figure 1-Figure 3 The present invention provides the application of royal jelly protein in Parkinson's disease drugs, wherein the technical scheme is as follows:

[0030] The application of royal jelly protein in Parkinson's disease drugs comprises the following steps:

[0031] Step 1: Extraction of royal jelly protein: crushing, filtering, centrifuging, precipitating and purifying the obtained royal jelly to obtain purified royal jelly protein;

[0032] Step 2, prepare raw materials according to the following raw material composition in parts by weight: 1.5 parts of Uncaria rhynchophylla, 1.-3.2 parts of Gastrodia elata, 2.2 parts of Tribulus terrestris, 0.8 parts of Semen Cassiae, 4.2 parts of Rehmannia glutinosa, 0.4 parts of Scorpio and 3.2 parts of Royal Jelly Apothecary;

[0033] Step 3, obtaining a medicinal solution, mixing and grinding the prepared Uncaria rhynchophylla, Gastrodia elata, Tribulus terrestris, and Semen Cassiae, grinding Rehmannia glutinosa and Scorpio glutinosa separately, and sieving them all through a 100-mesh sieve, decocting the sieved mixed powder with water for 1.5 hours, adding Rehmannia glutinosa and Scorpio glutinosa powder, continuing to decoct in water for 1.0 hour, filtering the medicinal residues, and obtaining a medicinal solution;

[0034] Step 4: Process the drug preparation, concentrate the drug solution into an extract, add the royal jelly main protein into the extract, stir, and vacuum dry to obtain a dry paste, crush the dry paste, and add pharmaceutically acceptable excipients to prepare a drug preparation commonly used in pharmacy.

[0035] In the above step 1, when the centrifugal treatment is carried out, the centrifugal equipment is kept running in a low temperature environment. During the purification process, the royal jelly protein is purified by ion exchange chromatography. The mutual charge interaction between the royal jelly protein and the ion exchange resin purifies and concentrates the royal jelly protein.

[0036] The centrifugal speed of the centrifugal device is controlled at 12000 rpm, and the centrifugal treatment is maintained at a temperature of 1°C.

[0037] The pharmaceutically acceptable excipients include starch, dextrin, magnesium stearate and microcrystalline cellulose.

[0038] The preparation is one of powder, decoction granules, tablets, capsules, oral liquid, pills or powders.

[0039] Example 2

[0040] See also Figure 1-Figure 3 The present invention provides the application of royal jelly protein in Parkinson's disease drugs, wherein the technical scheme is as follows:

[0041] The application of royal jelly protein in Parkinson's disease drugs comprises the following steps:

[0042] Step 1: Extraction of royal jelly protein: crushing, filtering, centrifuging, precipitating and purifying the obtained royal jelly to obtain purified royal jelly protein;

[0043] Step 2, prepare raw materials according to the following raw material composition by weight: 2.0 parts of Uncaria rhynchophylla, 3.2 parts of Gastrodia elata, 3.6 parts of Tribulus terrestris, 1.2 parts of Semen Cassiae, 5.0 parts of Rehmannia glutinosa, 1.6 parts of Scorpio and 4.8 parts of Royal Jelly Apothecary;

[0044] Step 3, obtaining a medicinal solution, mixing and grinding the prepared Uncaria rhynchophylla, Gastrodia elata, Tribulus terrestris, and Semen Cassiae, grinding Rehmannia glutinosa and Scorpio glutinosa separately, and sieving them all through a 120-mesh sieve, boiling the sieved mixed powder in water for 2.5 hours, adding Rehmannia glutinosa and Scorpio glutinosa powder, and continuing to boil in water for 1.5 hours, filtering the medicinal residues to obtain a medicinal solution;

[0045] Step 4: Process the drug preparation, concentrate the drug solution into an extract, add the royal jelly main protein into the extract, stir, and vacuum dry to obtain a dry paste, crush the dry paste, and add pharmaceutically acceptable excipients to prepare a drug preparation commonly used in pharmacy.

[0046] In the above step 1, when the centrifugal treatment is carried out, the centrifugal equipment is kept running in a low temperature environment. During the purification process, the royal jelly protein is purified by ion exchange chromatography. The mutual charge interaction between the royal jelly protein and the ion exchange resin purifies and concentrates the royal jelly protein.

[0047] The centrifugal speed of the centrifugal device is controlled at 3600 rpm, and the centrifugal treatment is maintained at a temperature of 3°C.

[0048] The pharmaceutically acceptable excipients include starch, dextrin, magnesium stearate and microcrystalline cellulose.

[0049] The preparation is one of powder, decoction, granules, tablets, capsules, oral liquid, pills or powder.

[0050] Example 3

[0051] See also Figure 1-Figure 3The preparation equipment of royal jelly protein in Parkinson's disease medicine of the present invention comprises a conical crushing box 1, a filter box 2, a transmission component 3, a crushing disc 4, a rubber grinding scraper 5, a filter tube 6, a push rod component 9, an end cover 7 and a filtrate sheet 8. The conical crushing box 1 is fixed on the filter box 2 and fixedly connected with the transmission component 3. The crushing disc 4 and the rubber grinding scraper 5 are both fixed on the lower end of the transmission component 3. The rubber grinding scraper 5 contacts the bottom of the conical crushing box 1. The filter tube 6 is fixed in the filter box 2 and connected with the lower end of the conical crushing box 1. The push rod component 9 is arranged on the filter tube 6. The end cover 7 is threadedly connected to the bottom of the filter tube 6 and is engaged with the filtrate sheet 8. The transmission component 3 installed on the conical crushing box 1 can drive the crushing disc 4 to crush the royal jelly raw material. The crushed royal jelly raw material can be ground by the rubber grinding scraper 5 to improve the fineness.

[0052] In this embodiment, preferably, the transmission assembly 3 includes a servo motor 31, a transmission shaft 32 and a cross support frame 33. The cross support frame 33 is fixed on the conical crushing box 1 and is rotatably connected to the transmission shaft 32 through a bearing. The crushing disc 4 and the rubber grinding scraper 5 are both fixed on the transmission shaft 32. The servo motor 31 is fixed on the conical crushing box 1, and the motor shaft is transmission-connected to the transmission shaft 32 through a coupling. The motor shaft of the servo motor 31 drives the transmission shaft 32 to rotate smoothly on the cross support frame 33 through a coupling, so that the crushing disc 4 and the rubber grinding scraper 5 run smoothly.

[0053] In this embodiment, preferably, a plurality of equally spaced flow holes 10 are provided at the lower end of the conical crushing box 1 , so that the royal jelly can flow out of the conical crushing box 1 through the flow holes 10 .

[0054] In this embodiment, preferably, the push rod assembly 9 includes an electric push rod 91 and a sealing piston plate 92. The electric push rod 91 is fixed on the filter tube 6, and the output end is fixedly connected to the sealing piston plate 92. The sealing piston plate 92 is sleeved in the filter tube 6. The electric push rod 91 can drive the sealing piston plate 92 to move in the filter tube 6, and the crushed royal jelly can be pushed to the bottom of the filter tube 6, so as to facilitate solid-liquid separation through the filtrate sheet 8 on the end cover 7.

[0055] In this embodiment, preferably, an injection port 11 is provided above the conical crushing box 1, and a sealing cover 12 is threadedly connected to the injection port 11. The injection port 11 facilitates the injection of royal jelly raw materials, and the sealing cover 12 reduces the entry of dust into the conical crushing box 1.

[0056] The working principle and use process of the present invention:

[0057] In the application of the royal jelly protein in Parkinson's disease drugs, the superoxide dismutase in the royal jelly protein is the main antioxidant component, which has a better nutritional supplement effect on people with malnutrition, weakness after illness and physical exhaustion in the elderly. It can also stimulate the production of glial cell-derived neurotrophic factor (GDNF) to play a neurotrophic effect on the mature brain. The enhanced expression of neurofilament HmRNA involves the subsequent neurotrophic factor GDNF. The royal jelly protein plays a neurotrophic or neuroprotective role in the mature brain through GDNF. The royal jelly protein makes the differentiation of all different types of brain cells easier, including neurons, astrocytes and oligodendrocytes. It also improves the function of neurons by promoting the regeneration of hippocampal granule cells (function in the cognitive process). The royal jelly protein plays the role of a promoter to activate the neural stem cells of the mature brain, thereby expecting it to differentiate into neurons and glial cells. While improving the effect of Parkinson's disease drug treatment, the royal jelly protein can also improve the patient's tolerance to the drug effect, thereby further improving the treatment effect.

[0058] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. The application of royal jelly protein in Parkinson's disease drugs, characterized in that: The following steps are involved: Step 1: Extraction of royal jelly protein: crushing, filtering, centrifuging, precipitating and purifying the obtained royal jelly to obtain purified royal jelly protein; Step 2, prepare raw materials according to the following raw material composition in parts by weight: 1.5-2.0 parts of Uncaria rhynchophylla, 1.8-3.2 parts of Gastrodia elata, 2.2-3.6 parts of Tribulus terrestris, 0.8-1.2 parts of Semen Cassiae, 4.2-5.0 parts of Rehmannia glutinosa, 0.4-1.6 parts of Scorpio and 3.2-4.8 parts of royal jelly protein; Step 3, obtaining a medicinal solution, mixing and grinding the prepared Uncaria rhynchophylla, Gastrodia elata, Tribulus terrestris, and Semen Cassiae, grinding Rehmannia glutinosa and Scorpio glutinosa separately, and sieving them all through a 100-120 mesh sieve, boiling the sieved mixed powder in water for 1.5-2.5 hours, adding Rehmannia glutinosa and Scorpio glutinosa powder, and continuing to boil in water for 1.0-1.5 hours, filtering the medicinal residues to obtain a medicinal solution; Step 4: Process the drug preparation, concentrate the drug solution into an extract, add the royal jelly main protein into the extract, stir, and vacuum dry to obtain a dry paste, crush the dry paste, and add pharmaceutically acceptable excipients to prepare a drug preparation commonly used in pharmacy.

2. The use of royal jelly protein in Parkinson's disease drugs according to claim 1, characterized in that: In the step 1, when the centrifugal treatment is performed, the centrifugal equipment is kept running in a low temperature environment. In the purification process, the royal jelly protein is purified by ion exchange chromatography. The mutual charge interaction between the royal jelly protein and the ion exchange resin purifies and concentrates the royal jelly protein.

3. The use of royal jelly protein in Parkinson's disease drugs according to claim 1, characterized in that: The centrifugal speed of the centrifugal device is controlled at 12000 rpm-3600 rpm, and the centrifugal treatment is maintained at a temperature environment of 1-3°C.

4. The use of royal jelly protein in Parkinson's disease drugs and the preparation equipment thereof according to claim 1, characterized in that: The pharmaceutically acceptable excipients include starch, dextrin, magnesium stearate and microcrystalline cellulose.

5. The use of royal jelly protein in Parkinson's disease drugs and the preparation equipment thereof according to claim 1, characterized in that: The preparation is one of powder, decoction, granule, tablet, capsule, oral liquid, pill or powder.

6. Equipment for preparing royal jelly protein in Parkinson's disease drugs, characterized by: The invention comprises a conical crushing box (1), a filter box (2), a transmission assembly (3), a crushing disc (4), a rubber grinding scraper (5), a filter tube (6), a push rod assembly (9), an end cover (7) and a filtrate sheet (8); the conical crushing box (1) is fixed on the filter box (2) and is fixedly connected to the transmission assembly (3); the crushing disc (4) and the rubber grinding scraper (5) are both fixed on the lower end of the transmission assembly (3); the rubber grinding scraper (5) is in contact with the bottom of the conical crushing box (1); the filter tube (6) is fixed in the filter box (2) and is connected to the lower end of the conical crushing box (1); the push rod assembly (9) is arranged on the filter tube (6); the end cover (7) is threadedly connected to the bottom of the filter tube (6) and is snap-connected to the filtrate sheet (8).

7. The preparation device of royal jelly protein in Parkinson's disease medicine according to claim 6, characterized in that: The transmission assembly (3) comprises a servo motor (31), a transmission shaft (32) and a cross support frame (33); the cross support frame (33) is fixed on the conical crushing box (1) and is rotationally connected to the transmission shaft (32) via a bearing; the crushing blade (4) and the rubber grinding scraper (5) are both fixed on the transmission shaft (32); the servo motor (31) is fixed on the conical crushing box (1), and the motor shaft is transmission-connected to the transmission shaft (32) via a coupling.

8. The preparation device of royal jelly protein in Parkinson's disease medicine according to claim 6, characterized in that: The lower end of the conical crushing box (1) is provided with a plurality of equally spaced flow holes (10).

9. The preparation device of royal jelly protein in Parkinson's disease medicine according to claim 6, characterized in that: The push rod assembly (9) comprises an electric push rod (91) and a sealing piston plate (92); the electric push rod (91) is fixed on the filter tube (6), and an output end is fixedly connected to the sealing piston plate (92); the sealing piston plate (92) is sleeved in the filter tube (6).

10. The preparation device of royal jelly protein in Parkinson's disease medicine according to claim 6, characterized in that: A material injection port (11) is provided above the conical crushing box (1), and a sealing cover (12) is threadedly connected to the material injection port (11).

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