Synergist, compound synergist, fertilizer and application thereof

Abstract

The invention belongs to the technical field of fertilizers and preparation thereof, and particularly relates to a synergist, a compound synergist, a fertilizer and application thereof, and the synergist comprises the following components: root exudates, vitamins and biostimulant. By adding the root exudates, the vitamins and the biostimulant, the synergist disclosed by the invention has the advantages of regulating and controlling arbuscular mycorrhizal fungi at the rhizosphere of crops, improving the phosphorus utilization rate of the crops, promoting the growth of the crops, improving the stress resistance of the crops, being low in cost and convenient to prepare, and meanwhile, improving the yield and quality of the crops and the like under the high-saline-alkali environmental condition; the application of chemical fertilizers is reduced, and the application range is wide.

Description

Technical Field

[0001] The invention belongs to the technical field of fertilizers and preparation thereof, and in particular relates to a synergist, a compound synergist and uses thereof. Background Art

[0002] Arbuscular mycorrhizal fungi (AMF) are widely present in the soil. They form a symbiotic relationship with plants by infecting the hyphae into the root cells. They have a close relationship with crops. Arbuscular mycorrhizal fungi can promote the absorption and transfer of essential nutrients such as nitrogen and phosphorus in the soil by crop roots. Related reports show that this mutually beneficial model between arbuscular mycorrhizal fungi and crops can improve the resistance of crops to abiotic stresses, such as salinity, drought, low temperature and other abiotic stresses; there are also reports that arbuscular mycorrhizal fungi can help crops better resist pathogenic microorganisms in the soil.

[0003] In order to increase crop yields and improve soil quality, various pesticides and fertilizers are often used in modern agriculture, which can provide the macro-elements and trace elements required by crops. In addition, microbial agents are used to improve soil structure and microbial activity. However, high salinity and alkaline conditions inhibit crop growth and root development, have a negative impact on mycorrhizal fungi, reduce the health of the soil, and thus reduce the effectiveness of fertilizers. At present, two methods are often used in the agricultural field to solve this problem. One is to directly use mycorrhizal fungal inoculants, and the other is to use synergists that can promote the growth of mycorrhizal fungi. The first method, by directly inoculating mycorrhizal fungi, can quickly establish mycorrhizal symbiotic relationships around plant roots, quickly improve the plant's ability to absorb nutrients, and long-term use can further improve the soil environment, but it has the problems of high cost and difficulty in survival in high saline-alkali environments. Compared with the former, the second method has the advantages of low cost, can still play a role in high saline-alkali environments, and can be used with chemical fertilizers to achieve better results. However, it still has the problems of unclear effects of specific synergistic substances and the need to optimize the formula.

[0004] Therefore, there is an urgent need to develop a synergist that is low-cost, easy to prepare, can regulate crop rhizosphere arbuscular mycorrhizal fungi, promote crop growth, improve crop stress resistance, is convenient for farmers to apply in the field, and is suitable for large-scale industrial application, so as to increase the yield and quality of crops and meet farmers' needs for functional synergists. Summary of the invention

[0005] The present invention aims to solve at least one of the technical problems existing in the prior art to a certain extent. To this end, the present invention provides a synergist, a compound synergist and its use. The synergist of the present invention, by adding root secretions, vitamins and biostimulants, has the advantages of being able to regulate crop rhizosphere arbuscular mycorrhizal fungi, improve crop utilization of phosphorus, promote crop growth, improve crop stress resistance, low cost, convenient preparation, and at the same time improve crop yield and quality, etc., which helps to reduce the application of chemical fertilizers and has a wide range of applications. The compound synergist of the present invention also has the advantages described in the aforementioned synergist, and is combined with Bacillus megaterium to further enhance the biological activity of the aforementioned synergist, improve the disease resistance of crops and the biological fertility of the soil.

[0006] In the first aspect of the present invention, the present invention proposes a synergist. According to an embodiment of the present invention, the synergist comprises the following components: root secretions, vitamins and biostimulants. The synergist of the present invention, by adding root secretions, vitamins and biostimulants, has the advantages of being able to regulate rhizosphere arbuscular mycorrhizal fungi of crops under high saline-alkali environmental conditions, improve the utilization rate of phosphorus by crops, promote crop growth, improve crop stress resistance, low cost, convenient preparation, and at the same time improve the yield and quality of crops, etc., which helps to reduce the application of chemical fertilizers and has a wide range of applications.

[0007] According to an embodiment of the present invention, the above-mentioned synergist may also have the following additional technical features:

[0008] According to an embodiment of the present invention, the root exudates include 1-monotetradecanoyl-RAC glycerol and / or ceramide.

[0009] According to an embodiment of the present invention, the vitamins include vitamin B12 and / or vitamin B6.

[0010] According to an embodiment of the present invention, the biostimulant includes one or more of trehalose, chitosan oligosaccharide, humic acid and alginic acid.

[0011] According to an embodiment of the present invention, the synergist comprises: 0.1 to 20 parts by weight of root exudates, 0.1 to 20 parts by weight of vitamins, and 20 to 98 parts by weight of biostimulants.

[0012] According to an embodiment of the present invention, the synergist includes: 20 to 98 parts by weight of trehalose, 20 to 98 parts by weight of chitosan oligosaccharides, 20 to 98 parts by weight of humic acid, 20 to 98 parts by weight of alginic acid, 0.1 to 20 parts by weight of vitamin B6, 0.1 to 20 parts by weight of vitamin B12, 0.1 to 20 parts by weight of 1-monotetradecanoyl-RAC glycerol, and 0.1 to 20 parts by weight of ceramide.

[0013] According to an embodiment of the present invention, the synergist includes: 25 to 50 parts by weight of trehalose, 20 to 40 parts by weight of chitosan oligosaccharides, 20 to 35 parts by weight of humic acid, 20 to 35 parts by weight of alginic acid, 0.1 to 5 parts by weight of vitamin B6, 0.1 to 5 parts by weight of vitamin B12, 0.1 to 2 parts by weight of 1-monotetradecanoyl-RAC glycerol, and 0.1 to 2 parts by weight of ceramide.

[0014] According to an embodiment of the present invention, the synergist comprises: 50 to 70 parts by weight of trehalose, 20 to 40 parts by weight of chitosan oligosaccharide, 0.1 to 0.5 parts by weight of vitamin B6, and 0.1 to 0.5 parts by weight of 1-monotetradecanoyl-RAC glycerol.

[0015] According to an embodiment of the present invention, the synergist comprises: 30 to 50 parts by weight of chitosan oligosaccharide, 30 to 50 parts by weight of alginic acid, 0.1 to 5 parts by weight of vitamin B6, and 0.1 to 5 parts by weight of ceramide.

[0016] According to an embodiment of the present invention, the synergist includes: 5 to 25 parts by weight of trehalose, 5 to 25 parts by weight of chitosan oligosaccharides, 5 to 23 parts by weight of humic acid, 5 to 25 parts by weight of alginic acid, 0.05 to 10 parts by weight of vitamin B6, 0.05 to 10 parts by weight of vitamin B12, 0.05 to 10 parts by weight of 1-monotetradecanoyl-RAC glycerol, and 0.05 to 10 parts by weight of ceramide.

[0017] According to an embodiment of the present invention, the synergist includes: 10 to 60 parts by weight of trehalose, 10 to 38 parts by weight of chitosan oligosaccharide, 0.1 to 20 parts by weight of vitamin B6, and 0.1 to 20 parts by weight of 1-monotetradecanoyl-RAC glycerol.

[0018] According to an embodiment of the present invention, the synergist comprises: 10 to 52 parts by weight of chitosan oligosaccharide, 10 to 46 parts by weight of alginic acid, 0.1 to 20 parts by weight of vitamin B6, and 0.1 to 20 parts by weight of ceramide.

[0019] According to an embodiment of the present invention, the synergist further includes auxiliary materials.

[0020] According to an embodiment of the present invention, the auxiliary materials include one or more of an anti-caking agent, stone powder, molasses powder, corn starch and water.

[0021] According to an embodiment of the present invention, based on 1 part by weight of the synergist, the added amount of the auxiliary material is ≤100 parts by weight of the synergist.

[0022] According to an embodiment of the present invention, based on 1 part by weight of the synergist, 20 to 50 parts by weight of molasses powder and / or 20 to 50 parts by weight of corn starch are added.

[0023] According to an embodiment of the present invention, based on 1 part by weight of the synergist, 20 to 40 parts by weight of molasses powder, 20 to 40 parts by weight of corn starch and 1 to 10 parts by weight of an anti-caking agent are added.

[0024] According to an embodiment of the present invention, based on 1 part by weight of the synergist, 20 to 40 parts by weight of stone powder, 20 to 40 parts by weight of corn starch and 1 to 10 parts by weight of an anti-caking agent are added.

[0025] In the second aspect of the present invention, the present invention proposes a compound synergist. According to an embodiment of the present invention, the compound synergist comprises the following components: a Bacillus megaterium agent and the synergist described in the first aspect. The compound synergist of the present invention also has the advantages described in the synergist of the first aspect, and is combined with Bacillus megaterium to further enhance the biological activity of the synergist of the first aspect, improve the disease resistance of crops and the biological fertility of the soil.

[0026] According to an embodiment of the present invention, the above-mentioned compound synergist may also have the following additional technical features:

[0027] According to an embodiment of the present invention, the Bacillus megaterium agent is added in the form of Bacillus megaterium liquid and / or Bacillus megaterium powder.

[0028] According to an embodiment of the present invention, based on 1 part by weight of the synergist described in the first aspect, the added amount of the Bacillus megaterium inoculant is 1 to 20 parts by weight, and the effective viable count of the Bacillus megaterium inoculant is ≥ 2 billion / (mL or g).

[0029] According to an embodiment of the present invention, the Bacillus megaterium inoculant comprises Bacillus megaterium with a deposit number of CGMCC No.21828.

[0030] According to an embodiment of the present invention, based on 1 part by weight of the synergist described in the first aspect, the added amount of the Bacillus megaterium inoculant is 1 to 20 parts by weight, and the effective viable count of the Bacillus megaterium inoculant is ≥ 2 billion / (mL or g).

[0031] In the third aspect of the present invention, the present invention provides a fertilizer. According to an embodiment of the present invention, the fertilizer comprises the synergist described in the first aspect and / or the compound synergist described in the second aspect.

[0032] Those skilled in the art will appreciate that the features and advantages described above for the synergist described in the first aspect and the compound synergist described in the second aspect are also applicable to this use and will not be described in detail herein.

[0033] According to an embodiment of the present invention, the above-mentioned fertilizer may also have the following additional technical features:

[0034] According to an embodiment of the present invention, the fertilizer includes one or more of compound fertilizer, inorganic fertilizer and organic fertilizer.

[0035] In the fourth aspect of the present invention, the present invention proposes the use of the synergist described in the first aspect and the compound synergist described in the second aspect in the preparation of a product. According to an embodiment of the present invention, the product has at least one of the following uses: regulating arbuscular mycorrhizal fungi in the rhizosphere of crops, promoting crop growth, and improving crop stress resistance.

[0036] Those skilled in the art will appreciate that the features and advantages described above for the synergist described in the first aspect, the compound synergist described in the second aspect, and the fertilizer described in the third aspect are also applicable to this use and will not be described in detail herein.

[0037] According to an embodiment of the present invention, the above-mentioned use may also have the following additional technical features:

[0038] According to an embodiment of the present invention, the crop includes one or more of corn, cotton, wheat and soybean. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0040] Figure 1 A table showing the results of the corn pot test in Example 3 of the present invention is drawn, wherein (A) is the aboveground biomass result of corn, (B) is the aboveground plant phosphorus uptake result, (C) is the spore density result, and (D) is the infection rate result;

[0041] Figure 2 The diagram is a microscope observation of corn root mycorrhizal fungi infection under different treatment conditions in Example 3 of the present invention, wherein (A) is the observation result of the CK0 group, (B) is the observation result of the CK group, (C) is the observation result of the Z1 group, (D) is the observation result of the Z2 group, and (E) is the observation result of the Z3 group;

[0042] Figure 3 A graph is made for the cotton pot test result table in Example 4 of the present invention, wherein (A) is the result of cotton boll yield per plant, (B) is the result of fruit branch number, (C) is the result of aboveground biomass, (D) is the result of spore density, (E) is the result of infection rate, and (F) is the result of plant phosphorus absorption;

[0043] Figure 4The microscopic observation diagram of cotton root mycorrhizal fungi infection under different treatment conditions in Example 4 of the present invention, wherein (A) is the observation result of the CK0 group, (B) is the observation result of the CK group, (C) is the observation result of the Z1 group, (D) is the observation result of the Z2 group, and (E) is the observation result of the Z3 group;

[0044] Figure 5 A table showing the results of the corn plot test in Example 6 of the present invention is drawn, wherein (A) is the yield result, (B) is the soil phosphatase result, (C) is the spore density result, and (D) is the infection rate result;

[0045] Figure 6 A graph is made for the cotton plot test result table in Example 7 of the present invention, wherein (A) is the yield result, (B) is the soil phosphatase result, (C) is the spore density result, and (D) is the infection rate result. DETAILED DESCRIPTION

[0046] The embodiments of the present invention are described in detail below. The embodiments described below are exemplary and are only used to explain the present invention, and should not be understood as limiting the present invention.

[0047] It should be noted that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. Further, in the description of the present invention, unless otherwise specified, the meaning of "plurality" is two or more.

[0048] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0049] In this document, the terms “include” or “comprising” are open expressions, that is, including the contents specified in the present invention but not excluding other contents.

[0050] As used herein, the terms "optionally", "optional" or "optionally" generally mean that the subsequently described event or circumstance may but need not occur, and that the description includes instances where the event or circumstance occurs and instances where it does not.

[0051] Synergist

[0052] The present invention proposes a synergist. According to an embodiment of the present invention, the synergist comprises the following components: root secretions, vitamins and biostimulants. The synergist of the present invention, by adding root secretions, vitamins and biostimulants, has the advantages of being able to regulate rhizosphere arbuscular mycorrhizal fungi of crops under high saline-alkali environmental conditions, improve the utilization rate of phosphorus by crops, promote crop growth, improve crop stress resistance, low cost, convenient preparation, and at the same time improve the yield and quality of crops, etc., helps to reduce the application of chemical fertilizers, and has a wide range of applications.

[0053] According to an embodiment of the present invention, the root secretion includes 1-monotetradecanoyl-RAC glycerol and / or ceramide. Thus, by adding specific root secretions, the adaptability of plants to adversity is enhanced, root development is promoted, and then the utilization rate of phosphorus, nutrient absorption efficiency, and healthy growth of crops are improved in collaboration with vitamins and biostimulants, so as to improve the yield and quality of crops. Exemplarily, 1-monotetradecanoyl-RAC glycerol (myristic acid monoglyceride) and ceramide, as components of small molecule root secretions, have a significant effect on rhizosphere microecology, and have the function of promoting mycorrhizal fungi, 1-monotetradecanoyl-RAC glycerol, by enhancing the fluidity and permeability of crop root cell membranes, thereby improving the absorption efficiency of crops to water and nutrients such as phosphorus, nitrogen, potassium, etc.; and ceramide, as an important component of cell membrane, helps to maintain the structure and function of cell membrane, protects crop roots from damage due to adversity, and participates in the immune response of plants, enhances the defense mechanism of crops to pathogens, and reduces the occurrence of crop diseases.

[0054] According to an embodiment of the present invention, the vitamins include vitamin B12 and / or vitamin B6. Thus, by adding multiple vitamins, the normal metabolic process of crops is assisted, the growth and development of crops is promoted, and then the utilization rate of phosphorus by crops, the efficiency of nutrient absorption, and the healthy growth of crops are improved in collaboration with root secretions and biostimulants, so as to improve the yield and quality of crops. Exemplarily, vitamin B6, as a coenzyme of multiple enzymes, plays a key role in amino acid metabolism. In addition, it is closely related to neurotransmitter synthesis, hemoglobin synthesis, hormone synthesis and energy metabolism; vitamin B12, as a coenzyme of methyltransferase, participates in the methylation reaction in the body. In addition, it is closely related to folic acid metabolism, hemoglobin synthesis and energy metabolism.

[0055] According to an embodiment of the present invention, the biostimulant includes one or more of trehalose, chitosan oligosaccharide, humic acid and alginic acid. Thus, by adding the biostimulant, the stress resistance and growth vitality of the crop are improved, and then the utilization rate of phosphorus by the crop is improved, the nutrient absorption efficiency is improved, and the healthy growth of the crop is promoted in cooperation with vitamins and root secretions, so as to improve the yield and quality of the crop. For example, trehalose, a natural substance extracted from seaweed, can significantly stimulate plant roots, improve the ability of functional roots to actively absorb trace elements, and improve the tolerance of crops to adversities such as drought, salinity, and cold; chitosan oligosaccharides, oligosaccharides extracted from chitin, can stimulate the crop's own defense mechanism to enhance resistance to diseases and pests, and improve the root structure to improve the efficiency of crop nutrition and water absorption. In addition, it can also act as a soil conditioner; humic acid can chelate trace elements in the soil, making it easier for crops to absorb, and can also improve the soil's water and fertilizer retention capacity; alginate and its degradation product, seaweed oligosaccharides, can regulate crop growth and induce crop disease resistance. In addition, the carboxyl and hydroxyl groups in alginate are easily combined with metal ions in the soil and promote the formation of soil granular structure and retain moisture, thereby improving crop yield and quality.

[0056] According to an embodiment of the present invention, the synergist comprises: 0.1 to 20 parts by weight of root secretions, 0.1 to 20 parts by weight of vitamins, and 20 to 98 parts by weight of biostimulants. Thus, by accurately proportioning the components, the synergist can be ensured to have a better effect while reducing costs, thereby improving the utilization rate of phosphorus by crops, improving nutrient absorption efficiency, and promoting healthy growth of crops, thereby improving the yield and quality of crops.

[0057] Exemplarily, the synergist includes 0.1 parts by weight of root exudates, 1 part by weight of root exudates, 5 parts by weight of root exudates, 10 parts by weight of root exudates, 15 parts by weight of root exudates, 20 parts by weight of root exudates, preferably 0.1 to 4 parts by weight of root exudates, and more preferably 2 parts by weight of root exudates; the synergist includes 0.1 parts by weight of vitamins, 1 part by weight of vitamins, 5 parts by weight of vitamins, 10 parts by weight of vitamins, 15 parts by weight of vitamins, 20 parts by weight of vitamins. The synergist comprises 20 parts by weight of biostimulants, 30 parts by weight of biostimulants, 40 parts by weight of biostimulants, 50 parts by weight of biostimulants, 60 parts by weight of biostimulants, 70 parts by weight of biostimulants, 80 parts by weight of biostimulants, 90 parts by weight of biostimulants, 98 parts by weight of biostimulants, preferably 20 to 95 parts by weight of biostimulants, more preferably 95 parts by weight of biostimulants.

[0058] According to an embodiment of the present invention, the synergist includes: 20 to 98 parts by weight of trehalose, 20 to 98 parts by weight of chitosan oligosaccharide, 20 to 98 parts by weight of humic acid, 20 to 98 parts by weight of alginic acid, 0.1 to 20 parts by weight of vitamin B6, 0.1 to 20 parts by weight of vitamin B12, 0.1 to 20 parts by weight of 1-monotetradecanoyl-RAC glycerol, and 0.1 to 20 parts by weight of ceramide. Thus, by accurately proportioning the components, while reducing costs, the synergist effect is ensured to be better, thereby improving the utilization rate of crops to phosphorus, improving nutrient absorption efficiency, and promoting healthy growth of crops, so as to improve the yield and quality of crops.

[0059] Exemplarily, the synergist includes 20 parts by weight of trehalose, 30 parts by weight of trehalose, 40 parts by weight of trehalose, 50 parts by weight of trehalose, 60 parts by weight of trehalose, 70 parts by weight of trehalose, 80 parts by weight of trehalose, 90 parts by weight of trehalose, 98 parts by weight of trehalose, preferably 20 to 25 parts by weight of trehalose, and more preferably 23 parts by weight of trehalose; the synergist includes 20 parts by weight of chitosan oligosaccharides, 30 parts by weight of chitosan oligosaccharides, 40 parts by weight of chitosan oligosaccharides, 50 parts by weight of chitosan oligosaccharides, 60 parts by weight of chitosan oligosaccharides, 70 parts by weight of chitosan oligosaccharides, 80 parts by weight of chitosan oligosaccharides, 90 parts by weight of chitosan oligosaccharides, 98 parts by weight of chitosan oligosaccharides, preferably 20 to 25 parts by weight of chitosan oligosaccharides. parts of chitosan oligosaccharide, more preferably 22 parts by weight of chitosan oligosaccharide; the synergist includes 20 parts by weight of humic acid, 30 parts by weight of humic acid, 40 parts by weight of humic acid, 50 parts by weight of humic acid, 60 parts by weight of humic acid, 70 parts by weight of humic acid, 80 parts by weight of humic acid, 90 parts by weight of humic acid, 98 parts by weight of humic acid, preferably 20-25 parts by weight of humic acid, more preferably 20 parts by weight of humic acid; the synergist includes 20 parts by weight of alginic acid, 30 parts by weight of alginic acid, 40 parts by weight of alginic acid, 50 parts by weight of alginic acid, 60 parts by weight of alginic acid, 70 parts by weight of alginic acid, 80 parts by weight of alginic acid, 90 parts by weight of alginic acid, 98 parts by weight of of alginic acid, preferably 20 to 25 parts by weight of alginic acid, more preferably 20 parts by weight of alginic acid; the synergist includes 0.1 parts by weight of vitamin B6, 1 part by weight of vitamin B6, 5 parts by weight of vitamin B6, 10 parts by weight of vitamin B6, 20 parts by weight of vitamin B6, preferably 1 to 10 parts by weight of vitamin B6, more preferably 3 parts by weight of vitamin B6; the synergist includes 0.1 parts by weight of vitamin B12, 1 part by weight of vitamin B12, 5 parts by weight of vitamin B12, 10 parts by weight of vitamin B12, 20 parts by weight of vitamin B12, preferably 1 to 10 parts by weight of vitamin B12, more preferably 3 parts by weight of vitamin B12; the synergist The enhancer comprises 0.1 parts by weight of 1-monotetradecanoyl-RAC glycerol, 1 part by weight of 1-monotetradecanoyl-RAC glycerol, 5 parts by weight of 1-monotetradecanoyl-RAC glycerol, 10 parts by weight of 1-monotetradecanoyl-RAC glycerol, 20 parts by weight of 1-monotetradecanoyl-RAC glycerol, preferably 2 to 10 parts by weight of 1-monotetradecanoyl-RAC glycerol, and more preferably 4 parts by weight of 1-monotetradecanoyl-RAC glycerol; the synergist comprises 0.1 parts by weight of ceramide, 1 part by weight of ceramide, 5 parts by weight of ceramide, 10 parts by weight of ceramide, and 20 parts by weight of ceramide, preferably 2 to 10 parts by weight of ceramide, and more preferably 5 parts by weight of ceramide.

[0060] According to an embodiment of the present invention, the synergist includes: 25 to 50 parts by weight of trehalose, 20 to 40 parts by weight of chitosan oligosaccharide, 20 to 35 parts by weight of humic acid, 20 to 35 parts by weight of alginic acid, 0.1 to 5 parts by weight of vitamin B6, 0.1 to 5 parts by weight of vitamin B12, 0.1 to 2 parts by weight of 1-monotetradecanoyl-RAC glycerol, and 0.1 to 2 parts by weight of ceramide. Thus, by accurately proportioning the components, while reducing costs, the synergist is ensured to have a better effect, thereby improving the utilization rate of phosphorus by crops, improving nutrient absorption efficiency, and promoting healthy growth of crops, so as to improve the yield and quality of crops.

[0061] Exemplarily, the synergist includes 20 parts by weight of trehalose, 30 parts by weight of trehalose, 40 parts by weight of trehalose, 50 parts by weight of trehalose, preferably 20 to 30 parts by weight of trehalose, and more preferably 25 parts by weight of trehalose; the synergist includes 20 parts by weight of chitosan oligosaccharides, 30 parts by weight of chitosan oligosaccharides, 40 parts by weight of chitosan oligosaccharides, preferably 20 to 25 parts by weight of chitosan oligosaccharides, and more preferably 22 parts by weight of chitosan oligosaccharides; the synergist includes 20 parts by weight of humic acid, 25 parts by weight of humic acid, 30 parts by weight of humic acid, 35 parts by weight of humic acid, preferably 25-30 parts by weight of humic acid, more preferably 28 parts by weight of humic acid; the synergist includes 20 parts by weight of alginic acid, 25 parts by weight of alginic acid, 30 parts by weight of alginic acid, 35 parts by weight of alginic acid, preferably 20-25 parts by weight of alginic acid, more preferably 21 parts by weight of alginic acid; the synergist includes 0.1 parts by weight of vitamin B6, 1 part by weight of vitamin B6, 5 parts by weight of vitamin B6, preferably 1-3 parts by weight of vitamin B6, more preferably 1.5 parts by weight of vitamin B6; the synergist includes 0.1 parts by weight of vitamin B12, 1 part by weight of vitamin B12, 5 parts by weight of vitamin B12, preferably 1-3 parts by weight of vitamin B12, more preferably 1.2 parts by weight of vitamin B12; the synergist includes 0.1 parts by weight of 1-monotetradecanoyl-RAC glycerol, 1 part by weight of 1-monotetradecanoyl-RAC glycerol, 2 parts by weight of 1-monotetradecanoyl-RAC glycerol, preferably 0.1-1 parts by weight of 1-monotetradecanoyl-RAC glycerol, more preferably 0.5 parts by weight of 1-monotetradecanoyl-RAC glycerol; the synergist includes 0.1 parts by weight of ceramide, 1 part by weight of ceramide, 2 parts by weight of ceramide, preferably 0.1-1 parts by weight of ceramide, more preferably 0.8 parts by weight of ceramide.

[0062] According to an embodiment of the present invention, the synergist comprises: 50 to 70 parts by weight of trehalose, 20 to 40 parts by weight of chitosan oligosaccharide, 0.1 to 0.5 parts by weight of vitamin B6, and 0.1 to 0.5 parts by weight of 1-monotetradecanoyl-RAC glycerol. Thus, by accurately proportioning the components, the effect of the synergist is ensured to be better while reducing the cost, thereby improving the utilization rate of phosphorus by crops, improving the efficiency of nutrient absorption, and promoting the healthy growth of crops, so as to improve the yield and quality of crops.

[0063] Exemplarily, the synergist includes 50 parts by weight of trehalose, 55 parts by weight of trehalose, 60 parts by weight of trehalose, preferably 60-70 parts by weight of trehalose, and more preferably 67 parts by weight of trehalose; the synergist includes 20 parts by weight of chitosan oligosaccharides, 25 parts by weight of chitosan oligosaccharides, 30 parts by weight of chitosan oligosaccharides, preferably 30-35 parts by weight of chitosan oligosaccharides, and more preferably 32.5 parts by weight of chitosan oligosaccharides; the synergist includes 0.1 parts by weight of vitamin B6, 0.2 parts by weight of vitamin B6, 0.3 parts by weight of vitamin B6, 0.4 parts by weight of vitamin B6, 0.5 parts by weight of Vitamin B6, preferably 0.2-0.4 parts by weight of vitamin B6, more preferably 0.3 parts by weight of vitamin B6; the synergist includes 0.1 parts by weight of 1-monotetradecanoyl-RAC glycerol, 0.2 parts by weight of 1-monotetradecanoyl-RAC glycerol, 0.3 parts by weight of 1-monotetradecanoyl-RAC glycerol, 0.4 parts by weight of 1-monotetradecanoyl-RAC glycerol, 0.5 parts by weight of 1-monotetradecanoyl-RAC glycerol, preferably 0.1-0.3 parts by weight of 1-monotetradecanoyl-RAC glycerol, more preferably 0.2 parts by weight of 1-monotetradecanoyl-RAC glycerol.

[0064] According to an embodiment of the present invention, the synergist comprises: 30 to 50 parts by weight of chitosan oligosaccharide, 30 to 50 parts by weight of alginic acid, 0.1 to 5 parts by weight of vitamin B6, and 0.1 to 5 parts by weight of ceramide. Thus, by accurately proportioning the components, the synergist can be ensured to have a better effect while reducing costs, thereby improving the utilization rate of phosphorus by crops, improving the efficiency of nutrient absorption, and promoting the healthy growth of crops, so as to improve the yield and quality of crops.

[0065] Exemplarily, the synergist includes 30 parts by weight of chitosan oligosaccharides, 40 parts by weight of chitosan oligosaccharides, 50 parts by weight of chitosan oligosaccharides, preferably 45-50 parts by weight of chitosan oligosaccharides, and more preferably 47 parts by weight of chitosan oligosaccharides; the synergist includes 35 parts by weight of alginic acid, 40 parts by weight of alginic acid, 45 parts by weight of alginic acid, 50 parts by weight of alginic acid, preferably 48-50 parts by weight of alginic acid, and more preferably 48 parts by weight of alginic acid; the synergist includes 0.1 parts by weight of vitamin B6, 1 part by weight of vitamin B6, 5 parts by weight of vitamin B6, preferably 1-5 parts by weight of vitamin B6, and more preferably 2 parts by weight of vitamin B6; the synergist includes 0.1 parts by weight of ceramide, 1 part by weight of ceramide, 5 parts by weight of ceramide, preferably 1-5 parts by weight of ceramide, and more preferably 3 parts by weight of ceramide.

[0066] According to a specific embodiment of the present invention, the synergist includes: 5 to 25 parts by weight of trehalose, 5 to 25 parts by weight of chitosan oligosaccharide, 5 to 23 parts by weight of humic acid, 5 to 25 parts by weight of alginic acid, 0.05 to 10 parts by weight of vitamin B6, 0.05 to 10 parts by weight of vitamin B12, 0.05 to 10 parts by weight of 1-monotetradecanoyl-RAC glycerol, and 0.05 to 10 parts by weight of ceramide. Thus, by accurately proportioning the components, while reducing costs, the synergist effect is ensured to be better, thereby improving the utilization rate of crops to phosphorus, improving nutrient absorption efficiency, and promoting healthy growth of crops, so as to improve the yield and quality of crops.

[0067] Exemplarily, the synergist includes 5 parts by weight of trehalose, 10 parts by weight of trehalose, 15 parts by weight of trehalose, 20 parts by weight of trehalose, 25 parts by weight of trehalose, preferably 15-25 parts by weight of trehalose, and more preferably 24 parts by weight of trehalose; the synergist includes 5 parts by weight of chitosan oligosaccharides, 10 parts by weight of chitosan oligosaccharides, 15 parts by weight of chitosan oligosaccharides, 20 parts by weight of chitosan oligosaccharides, and 25 parts by weight of chitosan oligosaccharides, preferably 10-20 parts by weight of chitosan oligosaccharides, and more preferably 17 parts by weight of chitosan oligosaccharides; the synergist includes 5 parts by weight of humic acid, 10 parts by weight of humic acid, 15 parts by weight of humic acid, 20 parts by weight of humic acid, 23 parts by weight of humic acid, preferably 13 to 23 parts by weight of humic acid, more preferably 22 parts by weight of humic acid; the synergist includes 5 parts by weight of alginic acid, 10 parts by weight of alginic acid, 15 parts by weight of alginic acid, 20 parts by weight of alginic acid, 25 parts by weight of alginic acid, preferably 15 to 25 parts by weight of alginic acid, more preferably 23 parts by weight of alginic acid; the synergist includes 0.05 parts by weight of vitamin B6, 0.1 parts by weight of vitamin B6, 1 part by weight of vitamin B6, 5 parts by weight of vitamin B6, 10 parts by weight of vitamin B6, preferably 5 to 10 parts by weight of vitamin B6, more preferably 8 parts by weight of vitamin B6; the synergist includes 0.05 parts by weight of vitamin B12, 0.1 parts by weight of vitamin B12, 1 part by weight of vitamin B12, 5 parts by weight of vitamin B12, 10 parts by weight of vitamin B12, preferably 5 to 10 parts by weight of vitamin B12, more preferably 5.3 parts by weight of vitamin B12; the synergist includes 0.05 parts by weight of 1-monotetradecanoyl-RAC glycerol, 0.1 parts by weight of 1-monotetradecanoyl-RAC glycerol, 1 part by weight of The synergist comprises 0.05 parts by weight of ceramide, 0.1 parts by weight of ceramide, 1 part by weight of ceramide, 5 parts by weight of 1-monotetradecanoyl-RAC glycerol, 10 parts by weight of 1-monotetradecanoyl-RAC glycerol, preferably 0.3-3 parts by weight of 1-monotetradecanoyl-RAC glycerol, more preferably 0.5 parts by weight of 1-monotetradecanoyl-RAC glycerol; the synergist comprises 0.05 parts by weight of ceramide, 0.1 parts by weight of ceramide, 1 part by weight of ceramide, 5 parts by weight of ceramide, 10 parts by weight of ceramide, preferably 0.05-5 parts by weight of ceramide, more preferably 0.2 parts by weight of ceramide.

[0068] According to an embodiment of the present invention, the synergist comprises: 10 to 60 parts by weight of trehalose, 10 to 38 parts by weight of chitosan oligosaccharide, 0.1 to 20 parts by weight of vitamin B6, and 0.1 to 20 parts by weight of 1-monotetradecanoyl-RAC glycerol. Thus, by accurately proportioning the components, the effect of the synergist is ensured to be better while reducing the cost, thereby improving the utilization rate of phosphorus by crops, improving the efficiency of nutrient absorption, and promoting the healthy growth of crops, so as to improve the yield and quality of crops.

[0069] Exemplarily, the synergist includes 10 parts by weight of trehalose, 20 parts by weight of trehalose, 30 parts by weight of trehalose, 40 parts by weight of trehalose, 50 parts by weight of trehalose, 60 parts by weight of trehalose, preferably 40-50 parts by weight of trehalose, and more preferably 45 parts by weight of trehalose; the synergist includes 10 parts by weight of chitosan oligosaccharides, 20 parts by weight of chitosan oligosaccharides, 30 parts by weight of chitosan oligosaccharides, 38 parts by weight of chitosan oligosaccharides, preferably 20-25 parts by weight of chitosan oligosaccharides, and more preferably 22 parts by weight of chitosan oligosaccharides; the synergist includes 0.1 parts by weight of vitamin B6, 1 part by weight of vitamin B6, 5 parts by weight of vitamin B6, 10 parts by weight of vitamin B6, 15 parts by weight of vitamin B6, 20 parts by weight of vitamin B6, preferably 15-20 parts by weight of vitamin B6, more preferably 16 parts by weight of vitamin B6; the synergist includes 0.1 parts by weight of 1-monotetradecanoyl-RAC glycerol, 1 part by weight of 1-monotetradecanoyl-RAC glycerol, 5 parts by weight of 1-monotetradecanoyl-RAC glycerol, 10 parts by weight of 1-monotetradecanoyl-RAC glycerol, 15 parts by weight of 1-monotetradecanoyl-RAC glycerol, 20 parts by weight of 1-monotetradecanoyl-RAC glycerol, preferably 15-20 parts by weight of 1-monotetradecanoyl-RAC glycerol, more preferably 17 parts by weight of 1-monotetradecanoyl-RAC glycerol.

[0070] According to an embodiment of the present invention, the synergist comprises: 10 to 52 parts by weight of chitosan oligosaccharide, 10 to 46 parts by weight of alginic acid, 0.1 to 20 parts by weight of vitamin B6, and 0.1 to 20 parts by weight of ceramide. Thus, by accurately proportioning the components, the synergist can be ensured to have a better effect while reducing costs, thereby improving the utilization rate of phosphorus by crops, improving the efficiency of nutrient absorption, and promoting the healthy growth of crops, so as to improve the yield and quality of crops.

[0071] Exemplarily, the synergist includes 10 parts by weight of chitosan oligosaccharide, 20 parts by weight of chitosan oligosaccharide, 30 parts by weight of chitosan oligosaccharide, 40 parts by weight of chitosan oligosaccharide, 50 parts by weight of chitosan oligosaccharide, 52 parts by weight of chitosan oligosaccharide, preferably 45-50 parts by weight of chitosan oligosaccharide, more preferably 47 parts by weight of chitosan oligosaccharide; the synergist includes 10 parts by weight of alginic acid, 20 parts by weight of alginic acid, 30 parts by weight of alginic acid, 40 parts by weight of alginic acid, 46 parts by weight of alginic acid, preferably 37-42 parts by weight of alginic acid, more preferably 40 parts by weight of alginic acid; the synergist includes 0.1 parts by weight of vitamin C. Vitamin B6, 1 part by weight of vitamin B6, 5 parts by weight of vitamin B6, 10 parts by weight of vitamin B6, 15 parts by weight of vitamin B6, 20 parts by weight of vitamin B6, preferably 10-15 parts by weight of vitamin B6, more preferably 12.5 parts by weight of vitamin B6; the synergist includes 0.1 parts by weight of ceramide, 1 part by weight of ceramide, 5 parts by weight of ceramide, 10 parts by weight of ceramide, 15 parts by weight of ceramide, 20 parts by weight of ceramide, preferably 0.1-1 parts by weight of ceramide, more preferably 0.5 parts by weight of ceramide.

[0072] According to an embodiment of the present invention, the synergist further comprises an auxiliary material. Thus, by adding the auxiliary material, the physical properties of the synergist are changed, so that it can be applied in the form of liquid, solid, powder, etc., and the stability and application convenience of the synergist product are improved, so that it is suitable for various application scenarios.

[0073] According to an embodiment of the present invention, the auxiliary materials include one or more of an anti-caking agent, stone powder, molasses powder, corn starch and water. Thus, by adding auxiliary materials, the physical properties of the synergist are changed, so that it can be used in the form of liquid, solid, powder, etc., and the stability and application convenience of the synergist product are improved, and it is suitable for various application scenarios. Exemplarily, the addition of an anti-caking agent prevents the powder or granular synergist from absorbing moisture and agglomerating during storage and transportation, maintains the free flow characteristics of the product, and extends the shelf life of the synergist product; molasses powder can improve the fluidity and dispersibility of the powder or granular synergist and reduce agglomeration; corn starch can adjust the viscosity of the product in the liquid synergist and improve its stability.

[0074] According to an embodiment of the present invention, based on 1 part by weight of the synergist, the amount of the auxiliary material added is ≤ 100 parts by weight of the synergist. Thus, the auxiliary material is added on the basis of ensuring the content and effect of the synergist, and while reducing the cost, the synergist effect is exerted to promote the growth and development of crops, improve the utilization rate of phosphorus by crops, improve the efficiency of nutrient absorption, and promote the healthy growth of crops, so as to improve the yield and quality of crops.

[0075] According to an embodiment of the present invention, based on 1 part by weight of the synergist, 20 to 50 parts by weight of molasses powder and / or 20 to 50 parts by weight of corn starch are added. Thus, by compounding the synergist with molasses powder and corn starch, the overall cost of the synergist is reduced while ensuring the fluidity and dispersibility of the synergist, and the synergist effect is exerted to promote the growth and development of crops, improve the utilization rate of phosphorus by crops, improve the efficiency of nutrient absorption, and promote the healthy growth of crops, so as to improve the yield and quality of crops.

[0076] Exemplarily, based on 1 part by weight of the synergist, 20 parts by weight of molasses powder, 30 parts by weight of molasses powder, 40 parts by weight of molasses powder, or 50 parts by weight of molasses powder are added, preferably 25 to 45 parts by weight of molasses powder, and more preferably 30 parts by weight of molasses powder; based on 1 part by weight of the synergist, 20 parts by weight of corn starch, 30 parts by weight of corn starch, 40 parts by weight of corn starch, or 50 parts by weight of corn starch are added, preferably 25 to 45 parts by weight of corn starch, and more preferably 25 parts by weight of corn starch.

[0077] According to an embodiment of the present invention, based on 1 part by weight of the synergist, 20 to 40 parts by weight of molasses powder, 20 to 40 parts by weight of corn starch and 1 to 10 parts by weight of an anti-caking agent are added. Thus, by compounding the synergist with molasses powder, corn starch and an anti-caking agent, while reducing the overall cost of the synergist, the fluidity and dispersibility of the synergist are guaranteed, ensuring that the ingredients are evenly distributed during application, exerting the synergist effect, promoting the growth and development of crops, improving the utilization rate of phosphorus by crops, improving the efficiency of nutrient absorption, and promoting the healthy growth of crops, so as to improve the yield and quality of crops.

[0078] Exemplarily, based on 1 part by weight of the synergist, 20 parts by weight of molasses powder, 30 parts by weight of molasses powder, 40 parts by weight of molasses powder, preferably 25 to 45 parts by weight of molasses powder, more preferably 30 parts by weight of molasses powder are added; based on 1 part by weight of the synergist, 20 parts by weight of corn starch, 30 parts by weight of corn starch, 40 parts by weight of corn starch are added, preferably 25 to 45 parts by weight of corn starch, more preferably 25 parts by weight of corn starch; based on 1 part by weight of the synergist, add 1 part by weight of an anti-caking agent, 2 parts by weight of an anti-caking agent, 3 parts by weight of an anti-caking agent, 4 parts by weight of an anti-caking agent, 5 parts by weight of an anti-caking agent, 6 parts by weight of an anti-caking agent, 7 parts by weight of an anti-caking agent, 8 parts by weight of an anti-caking agent, 9 parts by weight of an anti-caking agent, 10 parts by weight of an anti-caking agent, preferably 1 to 5 parts by weight of an anti-caking agent, more preferably 2 parts by weight of an anti-caking agent.

[0079] According to an embodiment of the present invention, based on 1 part by weight of the synergist, 20 to 40 parts by weight of stone powder, 20 to 40 parts by weight of corn starch and 1 to 10 parts by weight of an anti-caking agent are added. Thus, by compounding the synergist with stone powder, corn starch and an anti-caking agent, while reducing the overall cost of the synergist, the fluidity and dispersibility of the synergist are ensured, the soil structure is improved with the help of stone powder, and the components are evenly distributed during application, so as to exert the synergist effect, promote the growth and development of crops, improve the utilization rate of phosphorus by crops, improve the efficiency of nutrient absorption, and promote the healthy growth of crops, so as to improve the yield and quality of crops.

[0080] Exemplarily, based on 1 part by weight of the synergist, 20 parts by weight of stone powder, 30 parts by weight of stone powder, or 40 parts by weight of stone powder are added, preferably 20-35 parts by weight of stone powder, and more preferably 25 parts by weight of stone powder; based on 1 part by weight of the synergist, 20 parts by weight of corn starch, 30 parts by weight of corn starch, or 40 parts by weight of corn starch are added, preferably 25 parts by weight of corn starch, and more preferably 25 parts by weight of corn starch; based on 1 part by weight of the synergist, 1 part by weight of an anti-caking agent, 2 parts by weight of an anti-caking agent, 3 parts by weight of an anti-caking agent, 4 parts by weight of an anti-caking agent, 5 parts by weight of an anti-caking agent, 6 parts by weight of an anti-caking agent, 7 parts by weight of an anti-caking agent, 8 parts by weight of an anti-caking agent, 9 parts by weight of an anti-caking agent, or 10 parts by weight of an anti-caking agent are added, preferably 1-5 parts by weight of an anti-caking agent, and more preferably 2 parts by weight of an anti-caking agent.

[0081] Compound synergist

[0082] The present invention proposes a compound synergist. According to an embodiment of the present invention, the compound synergist comprises the following components: a Bacillus megaterium agent and the aforementioned synergist. The compound synergist of the present invention also has the advantages described in the aforementioned synergist, and is combined with Bacillus megaterium to further enhance the biological activity of the aforementioned synergist, improve the disease resistance of crops and the biological fertility of the soil.

[0083] According to an embodiment of the present invention, the Bacillus megaterium inoculant is added in the form of Bacillus megaterium liquid and / or Bacillus megaterium powder. Thus, by adding in the form of liquid or powder and in a precise amount, the effective live bacteria count of the inoculant is ensured, and the application effect of the synergist is improved.

[0084] According to an embodiment of the present invention, based on 1 part by weight of the aforementioned synergist, the addition amount of the Bacillus megaterium inoculant is 1 to 20 parts by weight, and the effective viable count of the Bacillus megaterium inoculant is ≥ 2 billion / (mL or g). Thus, while ensuring the effective viable count of Bacillus megaterium, the compound application effect of the synergist and the Bacillus megaterium inoculant is further improved.

[0085] Exemplarily, based on 1 part by weight of the aforementioned synergist, the effective live bacterial count of the Bacillus megaterium agent is ≥2 billion / (mL or g), the amount of the Bacillus megaterium agent added is 1 part by weight, the amount of the Bacillus megaterium agent added is 5 parts by weight, the amount of the Bacillus megaterium agent added is 10 parts by weight, the amount of the Bacillus megaterium agent added is 15 parts by weight, and the amount of the Bacillus megaterium agent added is 20 parts by weight. It is preferred to add 1 to 10 parts by weight of the Bacillus megaterium agent, and more preferably to add 5 parts by weight of the Bacillus megaterium agent.

[0086] According to an embodiment of the present invention, the Bacillus megaterium agent comprises Bacillus megaterium with a deposit number of CGMCC No. 21828. The patented strain of Bacillus megaterium with a deposit number of CGMCC No. 21828 has high salt tolerance, fast growth rate and passage stability, can promote crop growth in saline-alkali soil environment, and improve the rhizosphere environment. Therefore, by compounding with the aforementioned synergist, it is possible to achieve better promotion of crop growth and development, improve crop utilization of phosphorus, improve nutrient absorption efficiency, and promote healthy growth of crops, so as to improve crop yield and quality effects.

[0087] According to an embodiment of the present invention, based on 1 part by weight of the aforementioned synergist, the addition amount of the Bacillus megaterium inoculant is 1 to 20 parts by weight, and the effective viable count of the Bacillus megaterium inoculant is ≥ 2 billion / (mL or g). Thus, while ensuring the effective viable count of the patented Bacillus megaterium strain with a deposit number of CGMCC No. 21828, the compound application effect of the synergist and the Bacillus megaterium inoculant is further improved.

[0088] Exemplarily, based on 1 part by weight of the aforementioned synergist, the effective live bacteria count of the Bacillus megaterium patented strain with a deposit number of CGMCC No. 21828 in the Bacillus megaterium inoculant is ≥ 2 billion / (mL or g), the amount of the Bacillus megaterium inoculant added is 1 part by weight, the amount of the Bacillus megaterium inoculant added is 5 parts by weight, the amount of the Bacillus megaterium inoculant added is 10 parts by weight, the amount of the Bacillus megaterium inoculant added is 15 parts by weight, and the amount of the Bacillus megaterium inoculant added is 20 parts by weight, preferably 1 to 10 parts by weight of the Bacillus megaterium inoculant is added, and more preferably 5 parts by weight of the Bacillus megaterium inoculant is added.

[0089] fertilizer

[0090] The present invention provides a fertilizer. According to an embodiment of the present invention, the fertilizer includes the aforementioned synergist and / or the aforementioned compound synergist.

[0091] Those skilled in the art will appreciate that the features and advantages described above for the aforementioned synergist and the aforementioned compound synergist are also applicable to this use and will not be described in detail herein.

[0092] According to an embodiment of the present invention, the fertilizer includes one or more of compound fertilizer, inorganic fertilizer and organic fertilizer. Therefore, the aforementioned synergist and the aforementioned compound synergist are applicable to a variety of fertilizers and have a wide range of applications.

[0093] use

[0094] The present invention proposes the use of the aforementioned synergist and the aforementioned compound synergist in preparing a product. According to an embodiment of the present invention, the product has at least one of the following uses: regulating arbuscular mycorrhizal fungi in the rhizosphere of crops, promoting crop growth, and improving crop stress resistance.

[0095] Those skilled in the art will appreciate that the features and advantages described above for the aforementioned synergist, the aforementioned compound synergist, and the aforementioned fertilizer are also applicable to this use and will not be described in detail herein.

[0096] According to an embodiment of the present invention, the crop includes one or more of corn, cotton, wheat, and soybean. Therefore, the aforementioned synergist and the aforementioned compound synergist are applicable to a variety of crops and have a wide range of applications.

[0097] The scheme of the present invention will be explained below in conjunction with the embodiments. It will be appreciated by those skilled in the art that the following embodiments are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. Where specific techniques or conditions are not indicated in the embodiments, the techniques or conditions described in the literature in this area or the product specifications are used. The reagents or instruments used are not indicated by the manufacturer and are all conventional products that can be obtained commercially.

[0098] Example 1: Preparation of synergists Z1 to Z3

[0099] The components in Table 1 were weighed, crushed, mixed evenly and sieved to prepare synergists numbered Z1 to Z3 in powder form, which were named synergist Z1, synergist Z2 and synergist Z3 respectively.

[0100] Table 1 Components of synergists numbered Z1 to Z3

[0101]

[0102] Example 2: Effect of synergist Z1 on mycorrhizal fungi of corn root system in a potted experiment in saline-alkali soil

[0103] In order to further explore whether the synergist prepared in Example 1 of the present invention can regulate the rhizosphere arbuscular mycorrhizal fungi of crops under saline-alkali soil environment, the synergist numbered Z1 in Example 1 was used to carry out a potted test of corn seedlings. The dry weight of the potted soil was 10 kg, no base fertilizer was applied, the soil salinity was moderately saline-alkali, and the total amount of soluble salt in the soil was 0.52%. The control group was set to add water, trehalose, chitosan oligosaccharide, humic acid, alginic acid, vitamin B6, vitamin B12, 1-monotetradecanoyl-RAC glycerol, ceramide, trehalose and ceramide mixture, chitosan oligosaccharide and ceramide mixture, humic acid and vitamin B6 mixture, vitamin B12 + 1-monotetradecanoyl-RAC glycerol mixture. Six weeks after the start of the experiment, the biomass of each group of corn plants and the spore density in the rhizosphere soil were measured. The treatment conditions (seedling stage) of each control group and experimental group are shown in Table 2.

[0104] Table 2 Treatment conditions of each control group and experimental group (seedling stage)

[0105]

[0106]

[0107] Among them, the method for determining the biomass of corn plants is a weighing method, destructive sampling, and the aboveground part is dried at 65°C to constant weight before weighing the biomass of the corn plants; the method for determining the spore density of rhizosphere soil is a wet screening method, taking 10g of soil, adding 40mL of deionized water and shaking thoroughly, and then centrifuging at 5000rpm for 10min, pouring out the supernatant to remove the residual root residues in the soil, and then adding 40mL of 60% (mass volume ratio) sucrose solution, centrifuging at 5000rpm for 10min, pouring the supernatant into a 500-mesh sieve, rinsing into a culture dish with a pointed washing bottle filled with pure water, and counting under a microscope with a 50x field of view.

[0108] The results of the corn pot test (seedling stage) with different synergistic substances are shown in Table 3.

[0109] Table 3 Results of corn pot test with different synergistic substances (seedling stage)

[0110] Group No. Corn plant biomass (g) Spore density (spores / 10g soil) A0 9 62 A1 13 78 A2 15 82 A3 14 81 A4 12 69 A5 13 72 A6 11 78 A7 15 85 A8 13 92 A9 17 99 A10 16 95 A11 16 96 A12 18 109 Z1 22 126

[0111] The results showed that after the synergist Z1 was treated with maize seedling potted plants, the biomass increased by 144% and the spore density increased by 103% compared with the A0 group water treatment; after the A1-A8 group substances were treated with maize seedling potted plants, the biomass increased by 22%-67% and the spore density increased by 11%-48% compared with the A0 group water treatment; after the A9-A12 group mixed substances were treated with maize seedling potted plants, the biomass increased by 78%-100% and the spore density increased by 16%-60% compared with the A0 group water treatment; after the synergist Z1 was treated with maize seedling potted plants, the biomass increased by 47%-100% and the spore density increased by 37%-83% compared with the A1-A8 group substances. Compared with the A9-A12 group mixed substances, the biomass of the synergist Z1 treatment increased by 22%-38% and the spore density increased by 16%-60%.

[0112] The above results show that after single substances such as trehalose, chitosan oligosaccharides, and humic acid are compounded to form synergists, they can effectively increase corn biomass and mycorrhizal fungal spore density in saline-alkali soil environments, thereby promoting the growth of corn plants.

[0113] Example 3: Effects of synergists Z1-Z3 on maize rhizomycorrhizal fungi in a saline-alkali soil pot experiment

[0114] In order to further explore whether the synergist prepared in Example 1 of the present invention can regulate the rhizosphere arbuscular mycorrhizal fungi of crops in a saline-alkali soil environment, the synergists numbered Z1 to Z3 in Example 1 were used to carry out a corn pot test of synergists Z1 to Z3 in Linyi, Shandong. The soil salinity was moderately saline-alkali, and the total amount of soluble salt in the soil was 0.52%. The control group was set to add clean water (CK0) and humic acid (CK), and the experimental group was added with synergist Z1, synergist Z2, and synergist Z3, 10 pots per group, and the dry weight of all potted soils was 25 kg / pot. The base fertilizer was 25-10-10 / Cl compound fertilizer (manufacturer: Sinochem Shandong Fertilizer Co., Ltd., dosage 8 g / pot). The specific steps are as follows:

[0115] First, sufficient corn seeds were rinsed with clean water, then soaked in H2O2 for 30 min for disinfection, and germinated at 26°C in the dark. 25 kg of soil was added to the planting pot, and 5 germinated corn seeds were sown in each pot. The seedlings were thinned out 2 weeks after emergence, and one neatly grown corn plant was left in each pot. After harvest, the aboveground plant biomass, total phosphorus content, mycorrhizal fungal spore density, and mycorrhizal fungal infection rate were tested. The treatment conditions (corn) of each control group and experimental group are shown in Table 4.

[0116] Table 4 Treatment conditions of each control group and experimental group (corn)

[0117] Group No. Processing conditions Mode of administration CK0 Shimizu Clean water, 500ml / basin CK Humic acid 0.8g / pot, dissolved in 500mL water and then applied Z1 Synergist Z1 0.8g / pot, dissolved in 500mL water and then applied Z2 Synergist Z2 0.8g / pot, dissolved in 500mL water and then applied Z3 Synergist Z3 0.8g / pot, dissolved in 500mL water and then applied

[0118] Among them, the determination method for detecting the aboveground plant biomass is the weighing method, destructive sampling, and the aboveground part is dried at 65°C to constant weight before weighing the corn plant biomass; the determination method for the aboveground plant phosphorus absorption is the molybdenum antimony colorimetric method, the dried sample is crushed, digested with H2SO4-H2O2, the molybdenum antimony solution is colored, and the plant phosphorus concentration is measured colorimetrically at 490nm, and the product of phosphorus concentration and biomass is the aboveground phosphorus absorption; the determination method for the mycorrhizal fungal spore density is the wet screening method, taking 10g of soil, adding 40mL of deionized water, shaking thoroughly, and centrifuging at 5000rpm for 1 0min, pour out the supernatant to remove the residual root residues in the soil; add 40mL of 60% (mass volume ratio) sucrose solution, centrifuge at 5000rpm for 10min, pour the supernatant into a 500-mesh sieve, rinse into a culture dish with a pointed bottle filled with pure water, and count under a microscope at 50 times the field of view; the method for determining the mycorrhizal fungal infection rate is the trypan blue staining method, the root segments are cut into 1cm / segment, soaked in KOH, after a 90℃ water bath, the pH is adjusted with HCl solution, and after staining with trypan blue solution, 30 root segments are randomly selected for preparation and pressing, and the infection rate is detected under a microscope at 100 times.

[0119] The results of the corn pot test are shown in Table 5.

[0120] Table 5 Corn pot test results

[0121]

[0122] See the table of corn pot test results for drawing. Figure 1 .

[0123] Microscopic observation of mycorrhizal fungi infection in maize root systems under different treatment conditions is shown in Figure 2 .

[0124] The results showed that compared with the clean water treatment of the CK0 group, after the application of enhancers Z1~Z3, the aboveground biomass of corn plants increased by 34%~60%, the aboveground phosphorus absorption of corn plants increased by 36%~60%, the spore density of mycorrhizal fungi increased by 101%~114%, and the mycorrhizal fungus infection rate increased by 57%~93%; compared with the humic acid treatment of the CK group, the aboveground biomass of corn plants increased by 24%~48%, the aboveground phosphorus absorption of corn plants increased by 23%~45%, the spore density of mycorrhizal fungi increased by 56%~66%, and the mycorrhizal fungus infection rate increased by 47%~81%.

[0125] The above results show that: using the synergists Z1~Z3 prepared in Example 1 of the present invention, in a saline-alkali soil environment, compared with only applying clean water or applying humic acid, the application of synergists Z1~Z3 improves the aboveground plant biomass of corn plants, the aboveground phosphorus absorption of corn plants, the mycorrhizal fungal spore density, and the mycorrhizal fungal infection rate, indicating that the synergists Z1~Z3 can effectively promote the growth of corn.

[0126] Example 4: Effects of synergists Z1-Z3 on cotton rhizomycorrhizal fungi in saline-alkali soil potted experiments

[0127] In order to further explore whether the synergist prepared in Example 1 of the present invention can regulate crop rhizosphere arbuscular mycorrhizal fungi in a saline-alkali soil environment, the synergists numbered Z1 to Z3 in Example 1 were used to conduct a cotton pot test of synergists Z1 to Z3 in Linyi, Shandong. The soil salinity was moderately saline-alkali, and the total amount of soluble salt in the soil was 0.52%. The control group was set to add clean water (CK0) and humic acid (CK), and the experimental group was added with synergist Z1, synergist Z2, and synergist Z3, respectively. Each group had 10 pots, and the dry weight of all potted soils was 25 kg / pot. The base fertilizer was 25-10-10 / Cl compound fertilizer (manufacturer: Sinochem Shandong Fertilizer Co., Ltd., dosage 8 g / pot). The specific steps are as follows:

[0128] First, sufficient cotton seeds were rinsed with clean water, then soaked in H2O2 for 30 min for disinfection, and germinated at 26°C in the dark. 25 kg of soil was added to the planting pots, and 5 germinated cotton seeds were sown in each pot. The seedlings were thinned out 2 weeks after emergence, and one neatly grown cotton plant was left in each pot. Half of the pots were collected before flowering to measure the aboveground biomass and plant phosphorus absorption. The cotton bolls were harvested after maturity. After harvesting, the cotton boll yield, number of fruit branches, mycorrhizal fungal spore density, and mycorrhizal fungal infection rate were tested. The treatment conditions (cotton) of each control group and experimental group are shown in Table 6.

[0129] Table 6 Treatment conditions of each control group and experimental group (cotton)

[0130] Group No. Processing conditions Mode of administration CK0 Shimizu Clean water, 500mL / basin CK Humic acid 0.8g / pot, dissolved in 500mL water and then applied Z1 Synergist Z1 0.8g / pot, dissolved in 500mL water and then applied Z2 Synergist Z2 0.8g / pot, dissolved in 500mL water and then applied Z3 Synergist Z3 0.8g / pot, dissolved in 500mL water and then applied

[0131] The results of the cotton pot test are shown in Table 7.

[0132] Table 7 Cotton pot test results

[0133]

[0134] Cotton pot test results table is shown in the figure. Figure 3 .

[0135] Microscopic observation of mycorrhizal fungi infection in cotton roots under different treatment conditions is shown in Figure 4 .

[0136] The results showed that compared with the clean water treatment in the CK0 group, after the application of synergists Z1-Z3, the aboveground biomass of cotton plants before flowering increased by 36%-44%, the aboveground phosphorus absorption of cotton plants increased by 20%-35%, the cotton boll yield at maturity increased by 11-22%, the mycorrhizal fungal spore density increased by 75%-89%, and the mycorrhizal fungal infection rate increased by 45%-70%; compared with the humic acid treatment in the CK group, after the application of synergists Z1-Z3, the aboveground biomass of cotton plants before flowering increased by 30%-34%, the aboveground phosphorus absorption of cotton plants increased by 12%-25%, the cotton boll yield at maturity increased by 21%-36%, the mycorrhizal fungal spore density increased by 53%-66%, and the mycorrhizal fungal infection rate increased by 16%-36%.

[0137] The above results show that the synergists Z1 to Z3 prepared in Example 1 of the present invention, in a saline-alkali soil environment, compared with the application of only clean water and humic acid, the application of synergists Z1 to Z3 improves the aboveground biomass of cotton plants, the phosphorus absorption of plants, the cotton boll yield at maturity, the mycorrhizal fungal spore density, and the mycorrhizal fungal infection rate, indicating that the synergists Z1 to Z3 can effectively promote the growth of cotton.

[0138] Example 5: Preparation of synergists ZXJ1 to ZXJ9

[0139] According to the distribution ratio of each group in Table 8, synergists numbered ZXJ1 to ZXJ9 in liquid, powder and granule forms were prepared and named synergist ZXJ1, synergist ZXJ2, synergist ZXJ3, synergist ZXJ4, synergist ZXJ5, synergist ZXJ6, synergist ZXJ7, synergist ZXJ8 and synergist ZXJ9, respectively.

[0140] Among them, synergists ZXJ1~ZXJ3 are liquid synergists, and the preparation steps are weighing each component, mixing evenly, dissolving each component in water and stirring evenly; synergists ZXJ4~ZXJ6 are powder synergists, and the preparation steps are weighing each component, mixing evenly and sieving; synergists ZXJ7~ZXJ9 are granular synergists, and the preparation steps are weighing each component, mixing evenly, stirring, granulating, drying and sieving.

[0141] Table 8 Components of synergists No. ZXJ1 to ZXJ9

[0142]

[0143] Example 6: Corn plot test of synergists ZXJ1, ZXJ4 and ZXJ7 in saline-alkali soil

[0144] In order to further explore whether the synergist prepared in Example 5 of the present invention can regulate the rhizosphere arbuscular mycorrhizal fungi of crops in a saline-alkali soil environment, the synergists numbered ZXJ1, ZXJ4 and ZXJ7 in Example 5 were used. From April to August 2024, corn plot tests of synergists ZXJ1, ZXJ4 and ZXJ7 were carried out in Dongying City, Shandong Province. Before applying base fertilizer, soil samples of corn plots were taken for total soluble salt test. The test results showed that the total soluble salt content of the soil in the corn plot was 0.21%; the control group was set to add clean water (CK0) and humic acid (CK), and the experimental group was added with synergist ZXJ1, synergist ZXJ4 and synergist ZXJ7, respectively. All treated base fertilizers were applied with 25-10-10 / Cl compound fertilizer (manufactured by Sinochem Shandong Fertilizer Co., Ltd., with a dosage of 50 kg / mu), with 3 plots in each group, and each plot had an area of ​​100m 3 After harvest, corn yield, soil phosphatase activity, mycorrhizal fungal spore density and mycorrhizal fungal infection rate were tested.

[0145] The treatment conditions of each control group and experimental group (corn plot) are shown in Table 9.

[0146] Table 9 Treatment conditions of each control group and experimental group (corn plot)

[0147] Group No. Processing conditions Mode of administration CK0 Shimizu Clean water, dosage 5 liters / mu CK Humic acid 5 kg / mu, dissolved in 500 kg water, drip irrigation ZXJ1 Synergist ZXJ1 5L / mu, dissolved in 500 kg of water, drip irrigation ZXJ4 Synergist ZXJ4 5 kg / mu, mix evenly with base fertilizer and apply ZXJ7 Synergist ZXJ7 5 kg / mu, mix evenly with base fertilizer and apply

[0148] The results of the corn plot test are shown in Table 10.

[0149] Among them, the method for determining corn yield is theoretical yield measurement, using the "X" five-point sampling method, selecting a sampling point at each of the four corners and the middle of a corn field, and the sampling point is more than 5 meters away from the field. The number of plants per mu is calculated based on the row spacing and plant spacing. At each sampling point, one ear is picked every 5 trees, 10 ears are picked at each point, and a total of 50 ears are picked. The retrieved ears are manually peeled and threshed, and the total number of grains and the average number of grains per ear are calculated. From the removed corn kernels, 1,000 kernels are randomly selected and dried in the sun for 2 to 3 days to reduce the moisture content of the seeds to about 14%, and then the weight of 1,000 seeds is weighed to calculate the weight of 100 grains. Theoretical yield = number of ears per mu × number of grains per ear × weight of 100 grains × 0.85 / 100000. The method for determining soil phosphatase activity is colorimetric method, weighing 5g of soil in a 50mL volumetric flask, adding 1mL of toluene, and shaking gently for 15min. Then add 5mL disodium phenyl phosphate and 5mL corresponding buffer (pH8.5 NaHCO3 buffer) and culture at 37℃ for 24h. Add potassium ferrocyanide and 4-aminoantipyrine solution to the volumetric flask for color development, and measure the phosphatase activity of each sample at a wavelength of 570nm. The method for determining the density of mycorrhizal fungi spores is the wet sieving method. Take 10g of soil, add 40mL of deionized water, shake well, centrifuge at 5000rpm for 10min, pour out the supernatant to remove the residual root residues in the soil; add 40mL of 60% (mass volume ratio) sucrose solution, centrifuge at 5000rpm for 10min, pour the supernatant into a 500-mesh sieve, rinse the culture dish with a pointed bottle filled with deionized water, and count under a microscope with a 50x field of view. The method for determining the infection rate of mycorrhizal fungi is the trypan blue staining method. The root segments are cut into 1 cm / segment, soaked in KOH, and then water bathed at 90°C. The pH is adjusted with HCl solution. After staining with trypan blue solution, 30 root segments are randomly selected for slide preparation and the infection rate is detected under a microscope at 100 times.

[0150] Table 10 Corn plot test results

[0151]

[0152] See the table of corn plot test results for drawing. Figure 5 .

[0153] The results showed that compared with the clean water treatment in the CK0 group, after the application of enhancers ZXJ1, ZXJ4 and ZXJ7, the corn yield in the corn plot increased by 25% to 44%, the soil phosphatase activity increased by 30% to 50%, the mycorrhizal fungal spore density increased by 31% to 48%, and the mycorrhizal fungal infection rate increased by 23% to 44%; compared with the humic acid treatment in the CK group, after the application of enhancers ZXJ1, ZXJ4 and ZXJ7, the corn yield in the corn plot increased by 12% to 29%, the soil phosphatase activity increased by 11% to 27%, the mycorrhizal fungal spore density increased by 20% to 36%, and the mycorrhizal fungal infection rate increased by 7% to 25%.

[0154] The above results show that: using the synergists ZXJ1, ZXJ4 and ZXJ7 prepared in Example 5 of the present invention, in a saline-alkali soil environment, compared with only applying clean water or applying humic acid, the application of synergists ZXJ1, ZXJ4 and ZXJ7 improves the corn yield, soil phosphatase activity, mycorrhizal fungal spore density and mycorrhizal fungal infection rate of the corn plot, indicating that the synergists ZXJ1, ZXJ4 and ZXJ7 can effectively promote corn growth.

[0155] Example 7: Cotton plot test of synergists ZXJ1, ZXJ4 and ZXJ7 in saline-alkali soil

[0156] In order to further explore whether the synergist prepared in Example 5 of the present invention can regulate the rhizosphere arbuscular mycorrhizal fungi of crops in a saline-alkali soil environment, the synergists numbered ZXJ1, ZXJ4 and ZXJ7 in Example 5 were used. From April to October 2024, the synergists ZXJ1, ZXJ4 and ZXJ7 cotton plot tests were carried out in Aral, Xinjiang. Before fertilization, soil samples of the cotton plot were taken for a total soluble salt test. The test results showed that the total soluble salt content of the soil in the cotton plot was 0.35%; the control group was set to add clean water (CK0) and humic acid (CK), and the experimental group was added with synergist ZXJ1, synergist ZXJ4, and synergist ZXJ7, respectively. All treatments were treated with 25-10-10 / C1 compound fertilizer (manufactured by Sinochem Shandong Fertilizer Co., Ltd., with a dosage of 50 kg / mu), with 3 plots in each group, and each plot had an area of ​​100m 3 After harvest, cotton yield, soil phosphatase activity, mycorrhizal fungal spore density and mycorrhizal fungal infection rate were tested.

[0157] The treatment conditions of each control group and experimental group (cotton plot) are shown in Table 11.

[0158] Table 11 Treatment conditions of each control group and experimental group (cotton plot)

[0159] Group No. Processing conditions Mode of administration CK0 Shimizu Clean water, dosage 5 liters / mu CK Humic acid 5 kg / mu, dissolved in 500 kg water, drip irrigation ZXJ1 Synergist ZXJ1 5L / mu, dissolved in 500kg water, drip irrigation ZXJ4 Synergist ZXJ4 5 kg / mu, mix evenly with base fertilizer and apply ZXJ7 Synergist ZXJ7 5 kg / mu, mix evenly with base fertilizer and apply

[0160] The cotton yield was determined by theoretical yield measurement. Five representative sampling points were randomly selected in the cotton field according to uniform distribution. Three rows were selected at each sampling point, and each row was 19.2 m (100 m 2) In each sampling point, the number of plants, mature bolls, young bolls, and flocculent bolls were investigated, and the number of mature bolls, single boll weight, and lint percentage were calculated to calculate the yield of cotton per unit area. Seed cotton yield per mu (kg / mu) = harvest density (plants / mu) × average number of bolls per plant (per plant) × single boll weight (g) / 1000 × yield correction factor (90%). The method for determining soil phosphatase activity is colorimetric method. Weigh 5g of soil into a 50mL volumetric flask, add 1mL of toluene, and shake gently for 15min. Then add 5mL of disodium phenyl phosphate and 5mL of the corresponding buffer (pH 8.5 NaHCO3 buffer) and culture at 37°C for 24h. In the volumetric flask, potassium ferrocyanide and 4-aminoantipyrine solution were added for color development, and the phosphatase activity of each sample was measured at a wavelength of 570nm. The method for determining the density of mycorrhizal fungi spores is the method for determining the density of mycorrhizal fungi spores is. The method for determining the density of mycorrhizal fungi spores is the wet sieving method. Take 10g of soil, add 40mL of deionized water, shake well, centrifuge at 5000rpm for 10min, pour out the supernatant to remove the root residues remaining in the soil; add 40mL of 60% (mass volume ratio) sucrose solution, centrifuge at 5000rpm for 10min, pour the supernatant into a 500-mesh sieve, rinse into a culture dish with a pointed bottle filled with deionized water, and count under a microscope at 50 times the field of view. The method for determining the infection rate of mycorrhizal fungi is the trypan blue staining method. The root segments are cut into 1cm / segments, soaked in KOH, and after a 90°C water bath, the pH is adjusted with HCl solution. After staining with trypan blue solution, 30 root segments are randomly selected for slice preparation and compression, and the infection rate is detected under a microscope at 100 times.

[0161] The results of the cotton plot test are shown in Table 12.

[0162] Table 12 Cotton plot test results

[0163]

[0164] Cotton plot test result table is drawn in Figure 6 .

[0165] The results showed that compared with the clean water treatment in the CK0 group, after the application of synergists ZXJ1, ZXJ4 and ZXJ7, the cotton yield in the cotton plot increased by 54%-66%, the soil phosphatase activity increased by 54%-70%, the mycorrhizal fungal spore density increased by 34%-54%, and the mycorrhizal fungal infection rate increased by 41%-72%; compared with the humic acid treatment in the CK group, after the application of synergists ZXJ1, ZXJ4 and ZXJ7, the cotton yield in the cotton plot increased by 35%-45%, the soil phosphatase activity increased by 28%-56%, the mycorrhizal fungal spore density increased by 19%-36%, and the mycorrhizal fungal infection rate increased by 23%-49%.

[0166] The above results show that: using the synergists ZXJ1, ZXJ4 and ZXJ7 prepared in Example 5 of the present invention, in a saline-alkali soil environment, compared with only applying clean water or applying humic acid, the application of synergists ZXJ1, ZXJ4 and ZXJ7 improves the cotton yield, soil phosphatase activity, mycorrhizal fungal spore density and mycorrhizal fungal infection rate of the cotton plot, indicating that the synergists ZXJ1, ZXJ4 and ZXJ7 can effectively promote cotton growth.

[0167] Example 8: Application of synergist in bacterial agent

[0168] In order to further explore the application mode of the synergist prepared in Example 5 of the present invention in a saline-alkali soil environment, the synergists numbered ZXJ1, ZXJ4 and ZXJ7 in Example 5 were respectively compounded with Bacillus megaterium agents, wherein the commercially available Bacillus megaterium bacterial liquid / powder were purchased from Qingdao Wei Lan Biological Co., Ltd., and the synergist-added bacterial agents numbered ZXJ10, XJ12 and ZXJ14 were prepared, wherein the Bacillus megaterium CGMCC.21828 bacterial powder / liquid were all produced by Sinochem Agriculture (Linyi) R&D Center Co., Ltd., and the synergist-added bacterial agents numbered ZXJ11, XJ13 and ZXJ15 were prepared.

[0169] Among them, the liquid enhancer ZXJ1 prepared in Example 5 was compounded with the liquid Bacillus megaterium to obtain liquid inoculants, which were numbered ZXJ10 and ZXJ11 respectively; the powder enhancer ZXJ4 prepared in Example 5 was compounded with the liquid Bacillus megaterium to obtain liquid inoculants, which were numbered ZXJ12 and ZXJ13 respectively; the granular enhancer ZXJ7 prepared in Example 5 was compounded with the liquid Bacillus megaterium to obtain liquid inoculants, which were numbered ZXJ14 and ZXJ15 respectively.

[0170] Table 13 Components of synergists No. ZXJ10 to ZXJ15

[0171] serial number form Components ZXJ10 liquid 1 part ZXJ1, 2 parts commercially available Bacillus megaterium liquid (effective viable count 5 billion / ml) ZXJ11 liquid 1 part ZXJ1, 2 parts Bacillus megaterium CGMCC No.21828 bacterial powder (effective viable count 5 billion / ml) ZXJ12 powder 1 part ZXJ4, 2 parts commercially available Bacillus megaterium powder (effective viable count 5 billion / g) ZXJ13 powder 1 part ZXJ4, 2 parts Bacillus megaterium CGMCC No.21828 powder (effective live bacteria count 5 billion / g) ZXJ14 Particles 1 part ZXJ7, 2 parts commercially available Bacillus megaterium liquid (effective viable count 5 billion / ml) ZXJ15 Particles 1 part ZXJ7, 2 parts Bacillus megaterium CGMCC No.21828 bacterial powder (effective viable count 5 billion / ml)

[0172] Example 9: Effects of synergists ZXJ10-ZXJ15 on mycorrhizal fungi of maize and cotton in pot experiments

[0173] In order to further explore whether the synergist prepared in Example 8 of the present invention can regulate the rhizosphere arbuscular mycorrhizal fungi of crops in a saline-alkali soil environment, the synergists numbered ZXJ10-ZXJ15 in Example 8 were used to carry out potted tests of corn and cotton with synergists ZXJ10-ZXJ15 in a greenhouse in Linyi, Shandong. Before fertilization, the soil salinity was detected to be a moderate saline-alkali level, and the total amount of soluble salt in the soil was 0.52%. The control group was set to add clean water (CK0), commercially available Bacillus megaterium liquid (CK1) (purchased from Qingdao Weilan Biological Co., Ltd.), Bacillus megaterium CGMCC No.218283 (CK2) (manufactured by Sinochem Agriculture (Linyi) R&D Center Co., Ltd.), and the experimental group was added with synergists ZXJ10-ZXJ15. All treatments were treated with 25-10-10 / Cl compound fertilizer (manufacturer: Sinochem Shandong Fertilizer Co., Ltd., dosage: 8 g / pot, 10 pots per group). After harvest, the aboveground biomass of corn and cotton, the density of mycorrhizal fungi spores, and the activity of soil phosphatase were tested. The specific steps are as follows:

[0174] First, rinse enough corn and cotton seeds with clean water, then soak them in H2O2 for 30 minutes for disinfection, and germinate them in the dark at 26℃. Add 25 kg of soil to the planting pots, sow 5 germinated corn seeds or cotton seeds in each pot, thin out the seedlings 2 weeks after germination, leave one neatly grown seedling in each pot, and harvest and test them at 30 days.

[0175] The treatment conditions of each control group and experimental group (corn and cotton potted plants) are shown in Table 14.

[0176] Table 14 Treatment conditions of the control group and experimental group (corn and cotton potted plants)

[0177]

[0178] Among them, the method for determining the aboveground biomass of corn or cotton is the weighing method, destructive sampling, and the aboveground biomass of corn or cotton plants is weighed after the aboveground part is dried at 65°C to constant weight. The method for determining the density of mycorrhizal fungi spores is the wet sieving method. Take 10g of soil, add 40mL of deionized water, shake well, centrifuge at 5000rpm for 10min, pour out the supernatant to remove the residual root residues in the soil; add 40mL of 60% (mass volume ratio) sucrose solution, centrifuge at 5000rpm for 10min, pour the supernatant into a 500-mesh sieve, rinse with a pointed bottle filled with deionized water into a culture dish, and count under a microscope at 50 times the field of view. The method for determining the activity of soil phosphatase is the colorimetric method. Weigh 5g of soil in a 50mL volumetric flask, add 1mL of toluene, and shake gently for 15min. Then add 5mL of disodium phenyl phosphate and 5mL of the corresponding buffer (pH=8.5NaHCO3 buffer), and culture at 37°C for 24h. Potassium ferrocyanide and 4-aminoantipyrine solution were added to a volumetric flask for color development, and the phosphatase activity of each sample was measured at a wavelength of 570 nm.

[0179] The results of the corn pot test (synergists ZXJ10~ZXJ15) are shown in Table 15.

[0180] Table 15 Corn pot test results (synergist ZXJ10 ~ ZXJ15)

[0181]

[0182]

[0183] The results showed that compared with the clean water treatment in the CK0 group, after the application of synergists ZXJ10-ZXJ15, the aboveground biomass of corn increased by 48%-97%, the density of mycorrhizal fungal spores increased by 82%-106%, and the soil phosphatase activity increased by 22%-43%; compared with the commercially available Bacillus megaterium solution treatment in the CK1 group, after the application of synergists ZXJ10-ZXJ15, the aboveground biomass of corn increased by 42%-89%, the density of mycorrhizal fungal spores increased by 53%-73%, and the soil phosphatase activity increased by 11%-30%; compared with the Bacillus megaterium CGMCC No.21828 treatment in the CK2 group, after the application of synergists ZXJ10-ZXJ15, the aboveground biomass of corn increased by 28%-70%, the density of mycorrhizal fungal spores increased by 22%-39%, and the soil phosphatase activity increased by 7%-25%.

[0184] The above results show that the synergists ZXJ10 to ZXJ15 prepared in Example 8 of the present invention, in a saline-alkali soil environment, compared with the application of only water, the application of commercially available Bacillus megaterium solution, and the application of Bacillus megaterium CGMCC No. 21828, the application of synergists ZXJ10 to ZXJ15 increased the aboveground biomass of corn potted corn, the density of mycorrhizal fungal spores, and the soil phosphatase activity, indicating that the synergists ZXJ10 to ZXJ15 can effectively promote the growth of corn.

[0185] The results of the cotton pot test (synergists ZXJ10~ZXJ15) are shown in Table 16.

[0186] Table 16 Cotton pot test results (synergist ZXJ10 ~ ZXJ15)

[0187]

[0188] The results showed that compared with the clean water treatment in the CK0 group, after the application of synergists ZXJ10-ZXJ15, the aboveground biomass of cotton increased by 21%-47%, the density of mycorrhizal fungal spores increased by 60%-91%, and the soil phosphatase activity increased by 38%-72%; compared with the commercially available Bacillus megaterium solution treatment in the CK1 group, after the application of synergists ZXJ10-ZXJ15, the aboveground biomass of cotton increased by 15%-39%, the density of mycorrhizal fungal spores increased by 44%-77%, and the soil phosphatase activity increased by 20%-50%; compared with the Bacillus megaterium CGMCC No.21828 treatment in the CK2 group, after the application of synergists ZXJ10-ZXJ15, the aboveground biomass of cotton increased by 11%-35%, the density of mycorrhizal fungal spores increased by 19%-47%, and the soil phosphatase activity increased by 7%-34%.

[0189] The above results show that the synergists ZXJ10 to ZXJ15 prepared in Example 8 of the present invention, in a saline-alkali soil environment, compared with the application of only water, the application of commercially available Bacillus megaterium solution, and the application of Bacillus megaterium CGMCC No. 21828, the application of synergists ZXJ10 to ZXJ15 on the aboveground biomass of cotton potted cotton, the density of mycorrhizal fungal spores and the activity of soil phosphatase are all improved, indicating that the synergists ZXJ10 to ZXJ15 can effectively promote the growth of cotton.

[0190] Example 10 Effect of using synergist in fertilizer

[0191] In order to further explore whether the synergist prepared in the embodiment of the present invention can be used in fertilizers, the synergist numbered Z1 in Example 1 is used to prepare fertilizers to observe whether there is no compaction and flatulence phenomenon. The components and steps of preparing the fertilizer are as follows:

[0192] Liquid fertilizer ZXJ16: 5 parts of the synergist Z1 in Example 1 are mixed evenly with 15 parts of potassium dihydrogen phosphate, 15 parts of dipotassium hydrogen phosphate, 10 parts of yeast fermentation liquid, 20 parts of dispersant, and 35 parts of water to prepare a liquid fertilizer numbered ZXJ16.

[0193] Granular fertilizer ZXJ17: 5 parts of the synergist Z1 in Example 1 are mixed with 40 parts of urea, 20 parts of monoammonium phosphate, 15 parts of potassium chloride and 20 parts of stick soil. After mixing evenly, 5 parts of water are sprayed for drum granulation. After sieving, the biofertilizer numbered ZXJ17 is obtained.

[0194] BB fertilizer ZXJ18: 5 parts of the synergist ZXJ7 in Example 5 were added to 100 kg of commercially available 25-15-15 specification BB fertilizer (manufactured by Sinochem Shandong Fertilizer Co., Ltd., with a dosage of 50 kg / mu), and the mixture was evenly mixed and numbered ZXJ18.

[0195] The results showed that after six months of follow-up, the three fertilizers, ZXJ16, granular fertilizer ZXJ17 and BB fertilizer ZXJ18, showed no signs of compaction or bloating and could be used normally.

[0196] Example 11 Application effects of liquid fertilizer ZXJ16, granular fertilizer ZXJ17 and BB fertilizer ZXJ18 in corn and cotton plots

[0197] In order to further explore whether the fertilizer prepared in Example 10 of the present invention can regulate the rhizosphere arbuscular mycorrhizal fungi of crops in a saline-alkali soil environment, the liquid fertilizer ZXJ16, granular fertilizer ZXJ17 and BB fertilizer ZXJ18 in Example 10 were used. From April to October 2024, liquid fertilizer ZXJ16, granular fertilizer ZXJ17 and BB fertilizer ZXJ18 corn and cotton plot tests were carried out in Dongying, Shandong; soil samples of the plot were taken before fertilization for total soluble salt test, and the test results showed that the total soluble salt of the plot soil was 0.21%; the control group was set to fertilizer (CK0) without adding the corresponding synergist and commercially available mycorrhizal fungal agent SH (manufactured by Zhejiang Shijia Technology Co., Ltd.), and the experimental group was added with liquid fertilizer ZXJ16, each group had 3 plots, and each plot had an area of ​​50m 3 , corn or cotton was tested after harvest to test yield, mycorrhizal fungal spore density and soil phosphatase activity.

[0198] Among them, the blank control group of liquid fertilizer ZXJ16 is a fertilizer without adding corresponding synergists, and the preparation method is 15 parts of potassium dihydrogen phosphate, 15 parts of dipotassium hydrogen phosphate, 10 parts of yeast fermentation broth, 20 parts of dispersant, and 40 parts of water. After mixing evenly, it is prepared into liquid fertilizer, numbered CK0-ZXJ16, and the dosage is 50 kg / mu; the blank control group of granular fertilizer ZXJ17 is a fertilizer without adding corresponding synergists, and the preparation method is 40 parts of urea, 20 parts of monoammonium phosphate, 15 parts of potassium chloride, and 25 parts of stick soil. After mixing evenly, 5 parts of water are sprayed for drum granulation, and granular fertilizer is obtained by sieving, numbered CK0-ZXJ17, and the dosage is 50 kg / mu; the blank control group of BB fertilizer ZXJ18 is a fertilizer without adding corresponding synergists, which is a commercially available 25-15-15 specification BB fertilizer (manufacturer is Sinochem Shandong Fertilizer Co., Ltd.), and the dosage is 50 kg / mu;

[0199] The control group of liquid fertilizer ZXJ16 is a liquid fertilizer prepared with commercial mycorrhizal fungi agents. The preparation method is 5 parts of commercial mycorrhizal fungi agents SH, 15 parts of potassium dihydrogen phosphate, 15 parts of dipotassium hydrogen phosphate, 10 parts of yeast fermentation liquid, 20 parts of dispersant, and 35 parts of water. After mixing evenly, it is prepared into liquid fertilizer, numbered SH1, with a dosage of 50 kg / mu; the control group of granular fertilizer ZXJ17 is a granular fertilizer prepared with commercial mycorrhizal fungi agents. The preparation method is 5 parts of commercial mycorrhizal fungi agents SH, It was mixed with 40 parts of urea, 20 parts of monoammonium phosphate, 15 parts of potassium chloride and 20 parts of stick soil, mixed evenly, and then sprayed with 5 parts of water for drum granulation. The granular fertilizer was sieved and numbered SH2, with a dosage of 50 kg / mu. The control group of BB fertilizer ZXJ18 was a fertilizer prepared with commercially available mycorrhizal fungi agents, and the preparation method was as follows: adding 5 parts of commercially available mycorrhizal fungi agents SH to the commercially available 25-15-15 specification BB fertilizer, and the fertilizer was evenly mixed to obtain the fertilizer, numbered SH3, with a dosage of 50 kg / mu.

[0200] Among them, the method for determining corn yield is theoretical yield measurement, using the "X" five-point sampling method, selecting a sampling point at each of the four corners and the middle of a corn field, and the sampling point is more than 5 meters away from the field. The number of plants per mu is calculated based on the row spacing and plant spacing. At each sampling point, one ear is picked every 5 trees, 10 ears are picked at each point, and a total of 50 ears are picked. The retrieved ears are manually peeled and threshed to calculate the total number of grains and the average number of grains per ear. From the removed corn kernels, 1,000 kernels are randomly selected and dried in the sun for 2 to 3 days to reduce the moisture content of the seeds to about 14%. Then the weight of 1,000 seeds is weighed to calculate the weight of 100 kernels. Theoretical yield = number of ears per mu × number of kernels per ear × weight of 100 kernels × 0.85 / 100000. The method for determining cotton yield is theoretical yield measurement. Five representative sampling points are randomly selected in the cotton field according to a uniform distribution for sampling. Three rows are selected at each sampling point, and each row is 19.2m (100m 2) In each sampling point, the number of plants, mature bolls, young bolls, and flocculent bolls were investigated, and the number of mature bolls, single boll weight, and lint percentage were calculated to calculate the yield of cotton. Seed cotton yield per mu (kg / mu) = harvest density (plants / mu) × average number of mature bolls per plant (per plant) × single boll weight (g) / 1000 × yield correction coefficient (90%). The method for determining the density of mycorrhizal fungi spores is the wet sieving method. Take 10g of soil, add 40mL of deionized water, shake well, centrifuge at 5000rpm for 10min, pour out the supernatant to remove the residual root residues in the soil; add 40mL of 60% (mass volume ratio) sucrose solution, centrifuge at 5000rpm for 10min, pour the supernatant into a 500-mesh sieve, rinse into a culture dish with a pointed bottle filled with deionized water, and count under a microscope with a 50x field of view. The method for determining soil phosphatase activity is colorimetric method. Weigh 5g soil sample into a 50mL volumetric flask, add 1mL toluene, and shake gently for 15min. Then add 5mL disodium phenyl phosphate and 5mL corresponding buffer (pH=8.5NaHCO3 buffer), and incubate at 37℃ for 24h. Add potassium ferrocyanide and 4-aminoantipyrine solution to the volumetric flask for color development, and determine the phosphatase activity of each sample at a wavelength of 570nm.

[0201] The treatment conditions of each control group and experimental group (liquid fertilizer ZXJ16) are shown in Table 17.

[0202] Table 17 Treatment conditions of each control group and experimental group (liquid fertilizer ZXJ16)

[0203] Group No. Processing conditions Mode of administration CK0-ZXJ16 CK0-ZXJ16 50 kg / mu, drip irrigation ZXJ16 Liquid fertilizer ZXJ16 50 kg / mu, drip irrigation SH1 SH1 50 kg / acre, drip irrigation

[0204] The treatment conditions of each control group and experimental group (granular fertilizer ZXJ17) are shown in Table 18.

[0205] Table 18 Treatment conditions of each control group and experimental group (granular fertilizer ZXJ17)

[0206] Group No. Processing conditions Mode of administration CK0-ZXJ17 CK0-ZXJ17 50 kg / mu, applied as base fertilizer ZXJ17 Granular fertilizer ZXJ17 50 kg / mu, applied as base fertilizer SH2 SH2 50 kg / mu, applied as base fertilizer

[0207] The treatment conditions of each control group and experimental group (BB fertilizer ZXJ18) are shown in Table 19.

[0208] Table 19 Treatment conditions of each control group and experimental group (BB fertilizer ZXJ18)

[0209] Group No. Processing conditions Mode of administration CK0-ZXJ18 CK0-ZXJ18 50 kg / mu, applied as base fertilizer ZXJ18 BB fat ZXJ18 50 kg / mu, applied as base fertilizer SH3 SH3 50 kg / mu, applied as base fertilizer

[0210] (1) The results of the corn and cotton plot tests (synergist ZXJ16) are shown in Table 20.

[0211] Table 20 Corn and cotton plot test results (synergist ZXJ16)

[0212]

[0213]

[0214] The results showed that compared with the CK0-ZXJ16 treatment, after the application of liquid fertilizer ZXJ16, the yields of corn and cotton increased by 43% and 33%, respectively, the density of mycorrhizal fungal spores increased by 37% and 41%, respectively, and the soil phosphatase activity increased by 38% and 54%, respectively; compared with the SH1 treatment, after the application of liquid fertilizer ZXJ16, the yields of corn and cotton increased by 11% and 10%, respectively, the density of mycorrhizal fungal spores increased by 6% and 4%, respectively, and the soil phosphatase activity increased by 9% and 23%, respectively.

[0215] The above results show that the application of liquid fertilizer ZXJ16 prepared in Example 10 of the present invention, in a saline-alkali soil environment, compared with the application of only CKO-ZXJ16 and SH1, the application of liquid fertilizer ZXJ16 improves the yield of corn or cotton, the density of mycorrhizal fungal spores and the activity of soil phosphatase. Adding the synergist prepared in the embodiment of the present invention to the liquid fertilizer can effectively promote the growth of corn and cotton.

[0216] (2) The results of the corn and cotton plot tests (granular fertilizer ZXJ17) are shown in Table 21.

[0217] Table 21 Corn and cotton plot test results (granular fertilizer ZXJ17)

[0218]

[0219] The results showed that compared with the CK0-ZXJ17 treatment, after the application of granular fertilizer ZXJ17, the yields of corn and cotton increased by 35% and 22%, respectively, the density of mycorrhizal fungal spores increased by 28% and 24%, respectively, and the soil phosphatase activity increased by 38% and 26%, respectively; compared with the SH2 treatment, after the application of granular fertilizer ZXJ17, the yields of corn and cotton increased by 10% and 12%, respectively, the density of mycorrhizal fungal spores increased by 6% and 4%, respectively, and the soil phosphatase activity increased by 12% and 15%, respectively.

[0220] The above results show that the granular fertilizer ZXJ17 prepared in Example 10 of the present invention improves the yield of corn or cotton, the density of mycorrhizal fungal spores and the activity of soil phosphatase in saline-alkali soil compared with the application of only CKO-ZXJ17 and SH2. Adding the synergist prepared in the embodiment of the present invention to the granular fertilizer can effectively promote the growth of corn and cotton.

[0221] (3) The results of the corn and cotton plot tests (BB fertilizer ZXJ18) are shown in Table 22.

[0222] Table 22 Corn and cotton plot test results (BB fertilizer ZXJ18)

[0223]

[0224] The results showed that compared with the CK0-ZXJ17 treatment, after the application of BB fertilizer ZXJ18, the yields of corn and cotton increased by 23% and 30%, respectively, the density of mycorrhizal fungal spores increased by 61% and 55%, respectively, and the soil phosphatase activity increased by 41% and 37%, respectively; compared with the SH3 treatment, after the application of BB fertilizer ZXJ18, the yields of corn and cotton increased by 11% and 9%, respectively, the density of mycorrhizal fungal spores increased by 13% and 14%, respectively, and the soil phosphatase activity increased by 11% and 14%, respectively.

[0225] The above results show that the BB fertilizer ZXJ18 prepared in Example 10 of the present invention, in a saline-alkali soil environment, improves the yield of corn or cotton, the density of mycorrhizal fungal spores and the activity of soil phosphatase compared with the application of only CKO-ZXJ18 and SH3. Adding the synergist prepared in the embodiment of the present invention to the BB fertilizer can effectively promote the growth of corn and cotton.

[0226] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0227] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A synergist, characterized in that The synergist comprises the following components: Root exudates, vitamins and biostimulants.

2. The synergist according to claim 1, characterized in that The root exudates include 1-monotetradecanoyl-RAC glycerol and / or ceramide; Optionally, the vitamins include vitamin B12 and / or vitamin B6; Optionally, the biostimulant comprises one or more of trehalose, chitosan oligosaccharide, humic acid and alginic acid.

3. The synergist according to claim 1 or 2, characterized in that The synergist includes: 0.1 to 20 parts by weight of root exudates, 0.1 to 20 parts by weight of vitamins, 20 to 98 parts by weight of biostimulants.

4. The synergist according to claim 3, characterized in that The synergist includes: 20 to 98 parts by weight of trehalose, 20 to 98 parts by weight of chitosan oligosaccharide, 20 to 98 parts by weight of humic acid, 20 to 98 parts by weight of alginic acid, 0.1 to 20 parts by weight of vitamin B6, 0.1 to 20 parts by weight of vitamin B12, 0.1 to 20 parts by weight of 1-monotetradecanoyl-RAC glycerol, 0.1 to 20 parts by weight of ceramide; Preferably, the synergist comprises: 25 to 50 parts by weight of trehalose, 20 to 40 parts by weight of chitosan oligosaccharide, 20 to 35 parts by weight of humic acid, 20 to 35 parts by weight of alginic acid, 0.1 to 5 parts by weight of vitamin B6, 0.1 to 5 parts by weight of vitamin B12, 0.1 to 2 parts by weight of 1-monotetradecanoyl-RAC glycerol, 0.1 to 2 parts by weight of ceramide; Preferably, the synergist comprises: 50 to 70 parts by weight of trehalose, 20 to 40 parts by weight of chitosan oligosaccharide, 0.1-0.5 parts by weight of vitamin B6, 0.1-0.5 parts by weight of 1-monotetradecanoyl-RAC glycerol; Preferably, the synergist comprises: 30 to 50 parts by weight of chitosan oligosaccharide, 30 to 50 parts by weight of alginic acid, 0.1 to 5 parts by weight of vitamin B6, 0.1 to 5 parts by weight of ceramide.

5. The synergist according to claim 1 or 3, characterized in that the synergist comprises: 5 to 25 parts by weight of trehalose, 5 to 25 parts by weight of chitosan oligosaccharide, 5 to 23 parts by weight of humic acid, 5 to 25 parts by weight of alginic acid, 0.05 to 10 parts by weight of vitamin B6, 0.05 to 10 parts by weight of vitamin B12, 0.05 to 10 parts by weight of 1-monotetradecanoyl-RAC glycerol, 0.05 to 10 parts by weight of ceramide; Optionally, the synergist comprises: 10 to 60 parts by weight of trehalose, 10 to 38 parts by weight of chitosan oligosaccharide, 0.1 to 20 parts by weight of vitamin B6, 0.1 to 20 parts by weight of 1-monotetradecanoyl-RAC glycerol; Optionally, the synergist comprises: 10 to 52 parts by weight of chitosan oligosaccharide, 10 to 46 parts by weight of alginic acid, 0.1 to 20 parts by weight of vitamin B6, 0.1 to 20 parts by weight of ceramide.

6. The synergist according to claim 1, characterized in that the synergist further comprises an auxiliary material; Optionally, the auxiliary materials include one or more of an anti-caking agent, stone powder, molasses powder, corn starch and water; Optionally, based on 1 part by weight of the synergist, the added amount of the auxiliary material is ≤ 100 parts by weight of the synergist.

7. The synergist according to claim 6, characterized in that, based on 1 part by weight of the synergist, 20 to 50 parts by weight of molasses powder and / or 20 to 50 parts by weight of corn starch are added; Optionally, based on 1 part by weight of the synergist, 20 to 40 parts by weight of molasses powder, 20 to 40 parts by weight of corn starch and 1 to 10 parts by weight of an anti-caking agent are added; Optionally, based on 1 part by weight of the synergist, 20 to 40 parts by weight of stone powder, 20 to 40 parts by weight of corn starch and 1 to 10 parts by weight of an anti-caking agent are added.

8. A compound synergist, characterized in that the compound synergist comprises the following components: A Bacillus megaterium agent and the synergist according to any one of claims 1 to 7.

9. The composite synergist according to claim 8, characterized in that: The Bacillus megaterium agent is added in the form of Bacillus megaterium liquid and / or Bacillus megaterium powder; Optionally, based on 1 part by weight of the synergist according to any one of claims 1 to 7, the added amount of the Bacillus megaterium agent is 1 to 20 parts by weight, and the effective viable count of the Bacillus megaterium agent is ≥ 2 billion / (mL or g).

10. The composite synergist according to claim 8, characterized in that: The Bacillus megaterium agent comprises Bacillus megaterium with a deposit number of CGMCC No. 21828; Optionally, based on 1 part by weight of the synergist according to any one of claims 1 to 7, the added amount of the Bacillus megaterium inoculant is 1 to 20 parts by weight, and the effective viable count of the Bacillus megaterium inoculant is ≥ 2 billion / (mL or g).

11. A fertilizer, characterized in that: The fertilizer comprises the synergist according to any one of claims 1 to 7 and / or the compound synergist according to any one of claims 8 to 10; Optionally, the fertilizer includes one or more of compound fertilizer, inorganic fertilizer and organic fertilizer.

12. Use of the synergist according to any one of claims 1 to 7 or the compound synergist according to any one of claims 8 to 10 in preparing a product, characterized in that: The product has at least one of the following uses: Regulate arbuscular mycorrhizal fungi in the crop rhizosphere, promote crop growth and improve crop stress resistance; Optionally, the crops include one or more of corn, cotton, wheat and soybeans.

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