Liquid organic fertilizer capable of effectively reducing damping-off of jute and preparation method of liquid organic fertilizer

Abstract

The invention discloses a liquid organic fertilizer capable of effectively reducing jute damping-off and a preparation method thereof, and belongs to the technical field of organic fertilizers. The fertilizer is prepared from the following components in parts by weight: 100-150 parts of compound microbial fermentation bacteria liquid, 10-15 parts of sodium alginate, 100-130 parts of food processing plant wastewater, 150-200 parts of agricultural and forestry wastes, 5-10 parts of an active agent and 1-5 parts of a dispersing agent. The compound microorganism fermentation bacterial liquid is prepared from bacillus parlicheniformis, paenibacillus mucilaginosus MSSW03 and trichoderma koningii; the active agent is a burdock leaf extract; the dispersing agent is prepared from modified organic silicon and lauryl sodium sulfate according to the mass ratio of 3: 1. After being used, the organic fertilizer has a good prevention effect on damping-off frequently occurring in the jute planting process, meanwhile, sufficient nutrients easy to absorb can be provided for jute growth, and the yield and quality of jute are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of organic fertilizers, and in particular relates to a liquid organic fertilizer capable of effectively reducing jute damping-off disease and a preparation method thereof. Background Art

[0002] Jute (Latin name Corchorus capsularis L), belongs to the genus Jute of Tiliaceae, is a bast fiber crop, an annual herb, prefers warm and humid climate, and is a short-day crop. It is also known as fire hemp, green hemp, hemp, water hemp, wild hemp, round fruit jute, round capsule jute, bitter hemp leaf, cow mud grass, three beads grass, sky perilla, hemp bone, etc. It is a long, soft, and shiny plant fiber that can be woven into high-strength rough filaments. Jute fiber is one of the cheapest natural fibers, and its planting volume and wide range of uses are second only to cotton. It has the characteristics of fine and soft fiber, high strength, good moisture absorption, fast water loss, and good spinnability. It can be used as a textile raw material, industrial raw material, and biomass energy, etc., and has a wide range of uses. In addition to the great utilization value of its bark fiber, jute leaves are also used as vegetables and food. Some edible jute is a high-quality source of rich protein, vitamins (A, C, E) and certain hormone precursors, and is rich in minerals such as calcium and iron. Its multiple nutritional values ​​make it used in China. Different parts of the long-fruited jute leaves contain a series of bioactive compounds and show a variety of biological properties, such as diuretic, antipyretic, analgesic, tonic, antibacterial activity, anti-tumor, anti-diabetes and a large number of phenolic antioxidant compounds, as well as hypoglycemic, anti-obesity, and stomach protection functions. It is commonly used in folk treatment of cystitis, dysuria, fever, gonorrhea, pharyngitis, diarrhea and vomiting, so it is used as an important material for the recovery of high-value bioactives in candy, health care and medicine. Jute leaves are rich in secondary metabolites such as flavonoids and polyphenols, can be used to treat various diseases, and have high medicinal value. Phenolic compounds and flavonoid compounds extracted from long-fruited jute leaves have good antioxidant capacity and antibacterial activity, and can be used as potential chemical antioxidants and biological preservatives.

[0003] Jute damping-off (Thanatephorus cucumeris) is a common and serious disease in the seedling stage of jute. The pathogen is Rhizoctonia solani. After the seedlings emerge from the soil, the main roots and stem bases of the affected seedlings turn brown and appear wet and rotten. The lateral roots are almost completely rotten. The lesions are constricted, causing the seedlings to wither and die suddenly. In the middle and late stages, the pathogen secretes toxins and cell wall degrading enzymes, causing the base of the jute stems to constrict and rot, and eventually leading to sudden death, especially when it is rainy and cloudy. Jute damping-off occurs in all major jute areas in China, and is most common in East China and South China. With the expansion of jute cultivation area, the disease has a tendency to worsen year by year. Jute damping-off can lead to a reduction in the number of effective plants, thereby affecting the yield of jute; at the same time, the fiber quality of seriously affected jute plants will also decline, further affecting the yield and quality, directly causing serious economic losses to farmers who grow jute.

[0004] At present, the use of chemical agents is the most effective method for preventing and controlling yellow blight, but the long-term and excessive use of chemical agents has caused the cost of prevention and control to rise, the prevention effect to decline, and there are risks such as improper use that can easily lead to the decline of soil fertility, loss of soil nutrients, and environmental pollution, which cannot achieve the desired yield increase or disease prevention effect. Therefore, there is an urgent need for a more efficient and environmentally friendly product for promoting jute growth, improving yield and quality, and preventing and controlling yellow blight. Summary of the invention

[0005] The object of the present invention is to provide a liquid organic fertilizer which can effectively reduce damping-off disease of jute. After use, the organic fertilizer has a good preventive effect on damping-off disease which often occurs in the process of jute planting. At the same time, it can also provide sufficient nutrients that are easy to absorb for jute growth, thereby improving jute yield and quality.

[0006] In order to achieve the above technical objectives, the technical solution adopted by the present invention is: A liquid organic fertilizer capable of effectively reducing damping-off of jute is prepared from the following raw materials in parts by weight: 100-150 parts of composite microbial fermentation liquid, 10-15 parts of sodium alginate, 100-130 parts of waste water from a food processing plant, 150-200 parts of agricultural and forestry wastes, 5-10 parts of an active agent, and 1-5 parts of a dispersant; the composite microbial fermentation liquid is prepared from Bacillus paralicheniformis, Paenibacillus jelly-like MSSW03, and Trichoderma pseudokoningii; the active agent is a burdock leaf extract; the dispersant is a modified organic silicon and sodium dodecyl sulfate in a mass ratio of 3:1; the Bacillus paralicheniformis has a strain number of No. CGMCC 1.15832, a preservation date of September 20, 2016, purchased from the General Microbiological Center of the China Microbiological Culture Collection Administration, and does not need to be repeatedly preserved; the Paenibacillus jelly-like MSSW03 (Paenibacillus mucilaginosusMSSW03), which has been deposited in the China Center for Type Culture Collection as a patent procedure before the application date, with a deposit number of CCTCC NO:M 20231473 and a deposit date of August 14, 2023; the Trichoderma pseudokoningii strain is numbered CCTCC DF20081027, with a deposit date of October 11, 1994, and was purchased from the China Center for Type Culture Collection, without the need for repeated deposit.

[0007] Preferably, the composite microbial fermentation broth is prepared by the following method: (1) Bacillus paralicheniformis, Paenibacillus jelly-like MSSW03, and Trichoderma pseudokoningii were activated and cultured in LB liquid seed medium to obtain a concentration of 2×10 8 cfu / mL~3×10 8 cfu / mL of three seed solutions; (2) inoculating the Bacillus paralicheniformis seed liquid into the fermentation liquid medium I at an inoculum rate of 10%, and fermenting and culturing at 30° C. for 5 days to obtain the Bacillus paralicheniformis fermentation liquid; (3) Inoculate the seed liquid of Paenibacillus jelly-like MSSW03 into the fermentation liquid medium II at an inoculum rate of 8%, and ferment and culture at 28°C for 3 days to obtain the Paenibacillus jelly-like MSSW03 fermentation liquid; (4) inoculating the seed liquid of Trichoderma pseudokoningii into the fermentation liquid medium III at an inoculum rate of 10%, and fermenting and culturing at 28° C. for 3 days to obtain the Trichoderma pseudokoningii fermentation liquid; (5) Mix the fermentation broth of Bacillus paralicheniformis, the fermentation broth of Paenibacillus jelly MSSW03 and the fermentation broth of Trichoderma pseudokoningii in a volume ratio of 1:1:1.

[0008] Preferably, the LB liquid seed culture medium is composed of: 10 g / L tryptone, 5 g / L yeast extract, 5 g / L sodium chloride, the pH value is adjusted to 7.0-7.5, and sterilized at about 121° C. for 20 min.

[0009] Preferably, the fermentation liquid medium I is composed of: 5 g / L yeast powder, 15 g / L starch hydrolyzate, 15 g / L corn steep liquor, 0.05 g / L calcium carbonate, 0.05 g / L magnesium sulfate, 2 g / L potassium dihydrogen phosphate, the pH value is adjusted to 7.0, and sterilized at about 115°C for 20 min.

[0010] Preferably, the fermentation liquid medium II is composed of: 10 g / L glucose, 15 g / L starch, 0.5 g / L inositol phospholipids, 0.05 g / L ammonium sulfate, 0.02 g / L ferrous sulfate, and 0.5 g / L potassium dihydrogen phosphate, the pH value is adjusted to 7.0, and sterilized at about 115°C for 20 min.

[0011] Preferably, the fermentation liquid medium III is composed of: 10 g / L starch, 5 g / L peptone, 0.05 g / L potassium nitrate, 0.1 g / L dipotassium hydrogen phosphate, 0.05 g / L magnesium sulfate, 1 g / L sodium chloride, the pH value is adjusted to 7.0, and sterilized at about 115°C for 20 min.

[0012] Preferably, the modified silicone preparation method is as follows: mix cardanol glycidyl ether and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane in a molar ratio of 1:1 under nitrogen protection, heat to 80-85°C, reflux for 1-2h under magnetic stirring, and naturally cool to room temperature to obtain the modified silicone.

[0013] Preferably, the burdock leaf extract is prepared by the following method: drying the burdock leaves and crushing them to 100 mesh to obtain burdock leaf powder; mixing the burdock leaf powder with 50% ethanol solution at a solid-liquid ratio of 1g:30ml, extracting at a pressure of 400MPa for 15min, and centrifuging to obtain the burdock leaf extract.

[0014] The present invention also provides a method for preparing the above-mentioned liquid organic fertilizer that can effectively reduce jute damping-off disease, which comprises the following steps: Step 1: preparing composite microbial fermentation liquid; Step 2: Mix the agricultural and forestry waste and the wastewater from the food processing plant and perform anaerobically fermentation for 5 days to obtain the primary fermentation product; Step 3: Sterilize the primary fermentation product and mix it with the composite microbial fermentation liquid, adjust the temperature to 30-35°C for fermentation culture for 10-12 days, adjust the stirring speed to 150-200r / min and the ventilation volume to 1.5-2.0vvm during fermentation, filter after fermentation, and take the filtrate to obtain a mixed fermentation liquid; Step 4: prepare active agent and dispersant respectively, then mix them evenly with mixed fermentation liquid and sodium alginate, and pack them into portions.

[0015] The specific usage and dosage of the liquid organic fertilizer capable of effectively reducing jute damping-off disease of the present invention are as follows: the liquid organic fertilizer can be applied with water flushing, drip irrigation or direct spraying, and the usage amount is 5.0 kg / mu.

[0016] The present invention selects Bacillus paralicheniformis, Bacillus gelatinous and Trichoderma pseudokoningii as active microorganisms to prepare composite microbial fermentation liquid, wherein Bacillus paralicheniformis can secrete a variety of secondary metabolites with antibacterial effects, which can inhibit the growth of pathogenic bacteria; Bacillus gelatinous can dissolve phosphorus, release the insoluble phosphorus element in the soil, improve nutrient utilization and enhance the resistance of crops to diseases; Trichoderma pseudokoningii can produce a variety of active enzymes, polysaccharides and humic acid during the fermentation process, wherein humic acid is a complex organic compound, which has the characteristics of increasing soil organic matter content and soil water retention capacity, and humic acid can also increase the activity of plant stress resistance enzymes, thereby enhancing its own disease resistance. The composite microbial fermentation liquid of the present invention selects the most suitable fermentation liquid culture medium according to the characteristics of different strains, and ferments and cultures them separately to obtain the microbial liquid with the highest activity, and then mixes them in equal proportions for use, and the three dominant strains act synergistically to form a dominant bacterial community in the root system of the plant, more effectively inhibit the growth and reproduction of pathogenic bacteria, and reduce the chance of pathogenic bacteria infecting crops, thereby more effectively preventing and controlling jute damping-off disease.

[0017] Agricultural and forestry waste mainly includes straw, rice husk, edible fungus matrix, scraps, firewood, bark, peanut shells, twigs, peel, shavings, etc., which are an important biomass resource and renewable resource in nature. In the process of preparing the fertilizer of the present invention, agricultural and forestry waste and food processing plant wastewater are subjected to anaerobic fermentation. The organic structure of agricultural waste treated by anaerobic fermentation is simpler and easier to be fermented and converted into humic acid by subsequent microorganisms, thereby improving the yield and quality of humic acid. The present invention combines anaerobic fermentation with composite microbial aerobic fermentation, and the obtained liquid organic fertilizer is rich in nutrients that are easy to be absorbed by jute, enhances the disease resistance of jute, and improves the quality and yield of jute.

[0018] The active agent burdock leaf extract of the present invention contains a large amount of polyphenols, which can promote the growth and reproduction of beneficial microorganisms, thereby enhancing their activity, and can also protect microorganisms from oxidative damage, thereby improving their stability. The two components of the dispersant of the present invention can synergistically enhance the dispersibility, wettability and permeability of liquid organic fertilizer on the leaf surface, enhance the stability of the fertilizer, promote the absorption and utilization of nutrients by jute, and improve the yield and quality of jute.

[0019] The beneficial effects of the present invention are as follows: the liquid organic fertilizer prepared by the present invention has excellent control effect on jute damping-off disease, reduces the use of pesticides and chemical fertilizers, reduces input and significantly improves jute yield and quality, and has significant economic benefits; at the same time, the agricultural and forestry wastes and food processing plant wastewater used are all recycled resources, which improves the resource recycling rate, reduces environmental pollution, and protects the ecological environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The results of the SOD activity test in jute leaves after using fertilizers in different treatment groups of the present invention; Figure 2 The results of CAT activity test in jute leaves after using fertilizers in different treatment groups of the present invention; Figure 3 The results of the POD activity test in jute leaves after using fertilizers of different treatment groups of the present invention are shown. DETAILED DESCRIPTION

[0021] The technical solution of the present invention is further described below in conjunction with specific embodiments, but is not limited thereto.

[0022] Example 1 A liquid organic fertilizer capable of effectively reducing damping-off of jute is prepared from the following raw materials in parts by weight: 100 kg of composite microbial fermentation liquid, 10 kg of sodium alginate, 100 kg of wastewater from a food processing plant, 150 kg of agricultural and forestry waste, 5 kg of an active agent, and 1 kg of a dispersant; the composite microbial fermentation liquid is prepared from Bacillus paralicheniformis, Paenibacillus jelly-like MSSW03, and Trichoderma pseudokoningii; the active agent is a burdock leaf extract; the dispersant is a modified organic silicon and sodium dodecyl sulfate in a mass ratio of 3:1; the Bacillus paralicheniformis has a strain number of No. CGMCC 1.15832, a preservation date of September 20, 2016, and is purchased from the General Microbiological Center of the China National Committee for the Preservation of Microorganisms; the Paenibacillus jelly-like MSSW03 has a strain number of CCTCC No. M 20231473, the preservation date is August 14, 2023, and it is preserved in the China Center for Type Culture Collection; the Trichoderma pseudokoningii strain number is CCTCC DF 20081027, the preservation date is October 11, 1994, and it was purchased from the China Center for Type Culture Collection.

[0023] The composite microbial fermentation broth is prepared by the following method: (1) Bacillus paralicheniformis, Paenibacillus jelly-like MSSW03, and Trichoderma pseudokoningii were activated and cultured in LB liquid seed medium to obtain a concentration of 2×10 8 cfu / mL~3×10 8 cfu / mL of three seed solutions; (2) inoculating the Bacillus paralicheniformis seed liquid into the fermentation liquid medium I at an inoculum rate of 10%, and fermenting and culturing at 30° C. for 5 days to obtain the Bacillus paralicheniformis fermentation liquid; (3) Inoculate the seed liquid of Paenibacillus jelly-like MSSW03 into the fermentation liquid medium II at an inoculum rate of 8%, and ferment and culture at 28°C for 3 days to obtain the Paenibacillus jelly-like MSSW03 fermentation liquid; (4) inoculating the seed liquid of Trichoderma pseudokoningii into the fermentation liquid medium III at an inoculum rate of 10%, and fermenting and culturing at 28° C. for 3 days to obtain the Trichoderma pseudokoningii fermentation liquid; (5) Mix the fermentation broth of Bacillus paralicheniformis, the fermentation broth of Paenibacillus jelly MSSW03 and the fermentation broth of Trichoderma pseudokoningii in a volume ratio of 1:1:1.

[0024] The LB liquid seed culture medium is composed of: 10 g / L tryptone, 5 g / L yeast extract, and 5 g / L sodium chloride. The pH value is adjusted to 7.0-7.5 and sterilized at about 121° C. for 20 min.

[0025] The fermentation liquid medium I is composed of: 5 g / L yeast powder, 15 g / L starch hydrolyzate, 15 g / L corn steep liquor, 0.05 g / L calcium carbonate, 0.05 g / L magnesium sulfate, 2 g / L potassium dihydrogen phosphate, the pH value is adjusted to 7.0, and sterilized at about 115° C. for 20 min.

[0026] The fermentation liquid medium II is composed of: 10 g / L glucose, 15 g / L starch, 0.5 g / L inositol phospholipids, 0.05 g / L ammonium sulfate, 0.02 g / L ferrous sulfate, and 0.5 g / L potassium dihydrogen phosphate. The pH value is adjusted to 7.0 and sterilized at about 115° C. for 20 min.

[0027] The fermentation liquid medium III is composed of: 10 g / L starch, 5 g / L peptone, 0.05 g / L potassium nitrate, 0.1 g / L dipotassium hydrogen phosphate, 0.05 g / L magnesium sulfate, and 1 g / L sodium chloride. The pH value is adjusted to 7.0 and sterilized at about 115° C. for 20 min.

[0028] The modified silicone preparation method is as follows: cardanol glycidyl ether and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane are mixed in a molar ratio of 1:1 under nitrogen protection, heated to 80° C., refluxed for 2 hours under magnetic stirring, and naturally cooled to room temperature to obtain the modified silicone.

[0029] The burdock leaf extract is prepared by the following method: drying the burdock leaves and then crushing them into 100 meshes to obtain burdock leaf powder; mixing the burdock leaf powder with 50% ethanol solution at a solid-liquid ratio of 1g:30ml, extracting at a pressure of 400MPa for 15min, and centrifuging to obtain a liquid that is the burdock leaf extract.

[0030] A method for preparing the above-mentioned liquid organic fertilizer that can effectively reduce jute damping-off disease comprises the following steps: Step 1: preparing composite microbial fermentation liquid; Step 2: Mix the agricultural and forestry waste and the wastewater from the food processing plant and perform anaerobically fermentation for 5 days to obtain the primary fermentation product; Step 3: Sterilize the primary fermentation product and mix it with the composite microbial fermentation liquid, adjust the temperature to 35°C for 12 days, adjust the stirring speed to 200r / min and the ventilation volume to 2.0vvm during fermentation, filter after the fermentation is completed, and take the filtrate to obtain a mixed fermentation liquid; Step 4: prepare active agent and dispersant respectively, then mix them evenly with mixed fermentation liquid and sodium alginate, and pack them into portions.

[0031] Example 2 A liquid organic fertilizer capable of effectively reducing damping-off of jute is prepared from the following raw materials in parts by weight: 150 kg of composite microbial fermentation liquid, 15 kg of sodium alginate, 130 kg of waste water from a food processing plant, 200 kg of agricultural and forestry waste, 10 kg of an active agent, and 5 kg of a dispersant; the composite microbial fermentation liquid is prepared from Bacillus paralicheniformis, Paenibacillus jelly-like MSSW03, and Trichoderma pseudokoningii; the active agent is a burdock leaf extract; the dispersant is a modified organic silicon and sodium dodecyl sulfate in a mass ratio of 3:1; the Bacillus paralicheniformis has a strain number of No. CGMCC 1.15832, a preservation date of September 20, 2016, and is purchased from the General Microbiological Center of the China National Committee for the Preservation of Microorganisms; the Paenibacillus jelly-like MSSW03 has a strain number of CCTCC No. M 20231473, the preservation date is August 14, 2023, and it is preserved in the China Center for Type Culture Collection; the Trichoderma pseudokoningii strain number is CCTCC DF 20081027, the preservation date is October 11, 1994, and it was purchased from the China Center for Type Culture Collection.

[0032] The composite microbial fermentation broth is prepared by the following method: (1) Bacillus paralicheniformis, Paenibacillus jelly-like MSSW03, and Trichoderma pseudokoningii were activated and cultured in LB liquid seed medium to obtain a concentration of 2×10 8 cfu / mL~3×10 8 cfu / mL of three seed solutions; (2) inoculating the Bacillus paralicheniformis seed liquid into the fermentation liquid medium I at an inoculum rate of 10%, and fermenting and culturing at 30° C. for 5 days to obtain the Bacillus paralicheniformis fermentation liquid; (3) Inoculate the seed liquid of Paenibacillus jelly-like MSSW03 into the fermentation liquid medium II at an inoculum rate of 8%, and ferment and culture at 28°C for 3 days to obtain the Paenibacillus jelly-like MSSW03 fermentation liquid; (4) inoculating the seed liquid of Trichoderma pseudokoningii into the fermentation liquid medium III at an inoculum rate of 10%, and fermenting and culturing at 28° C. for 3 days to obtain the Trichoderma pseudokoningii fermentation liquid; (5) Mix the fermentation broth of Bacillus paralicheniformis, the fermentation broth of Paenibacillus jelly MSSW03 and the fermentation broth of Trichoderma pseudokoningii in a volume ratio of 1:1:1.

[0033] The LB liquid seed culture medium is composed of: 10 g / L tryptone, 5 g / L yeast extract, and 5 g / L sodium chloride. The pH value is adjusted to 7.0-7.5 and sterilized at about 121° C. for 20 min.

[0034] The fermentation liquid medium I is composed of: 5 g / L yeast powder, 15 g / L starch hydrolyzate, 15 g / L corn steep liquor, 0.05 g / L calcium carbonate, 0.05 g / L magnesium sulfate, 2 g / L potassium dihydrogen phosphate, the pH value is adjusted to 7.0, and sterilized at about 115° C. for 20 min.

[0035] The fermentation liquid medium II is composed of: 10 g / L glucose, 15 g / L starch, 0.5 g / L inositol phospholipids, 0.05 g / L ammonium sulfate, 0.02 g / L ferrous sulfate, and 0.5 g / L potassium dihydrogen phosphate. The pH value is adjusted to 7.0 and sterilized at about 115° C. for 20 min.

[0036] The fermentation liquid medium III is composed of: 10 g / L starch, 5 g / L peptone, 0.05 g / L potassium nitrate, 0.1 g / L dipotassium hydrogen phosphate, 0.05 g / L magnesium sulfate, and 1 g / L sodium chloride. The pH value is adjusted to 7.0 and sterilized at about 115° C. for 20 min.

[0037] The modified silicone preparation method is as follows: cardanol glycidyl ether and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane are mixed in a molar ratio of 1:1 under nitrogen protection, heated to 85° C., refluxed for 1 hour under magnetic stirring, and naturally cooled to room temperature to obtain the modified silicone.

[0038] The burdock leaf extract is prepared by the following method: drying the burdock leaves and then crushing them into 100 meshes to obtain burdock leaf powder; mixing the burdock leaf powder with 50% ethanol solution at a solid-liquid ratio of 1g:30ml, extracting at a pressure of 400MPa for 15min, and centrifuging to obtain a liquid that is the burdock leaf extract.

[0039] A method for preparing the above-mentioned liquid organic fertilizer that can effectively reduce jute damping-off disease comprises the following steps: Step 1: preparing composite microbial fermentation liquid; Step 2: Mix the agricultural and forestry waste and the wastewater from the food processing plant and perform anaerobically fermentation for 5 days to obtain the primary fermentation product; Step 3: Sterilize the primary fermentation product and mix it with the composite microbial fermentation liquid, adjust the temperature to 30°C for 10 days, adjust the stirring speed to 150r / min and the ventilation volume to 1.5vvm during fermentation, filter after the fermentation is completed, and take the filtrate to obtain a mixed fermentation liquid; Step 4: prepare active agent and dispersant respectively, then mix them evenly with mixed fermentation liquid and sodium alginate, and pack them into portions.

[0040] Example 3 A liquid organic fertilizer capable of effectively reducing damping-off of jute is prepared from the following raw materials in parts by weight: 120 kg of composite microbial fermentation liquid, 12 kg of sodium alginate, 120 kg of waste water from a food processing plant, 180 kg of agricultural and forestry waste, 8 kg of an active agent, and 3 kg of a dispersant; the composite microbial fermentation liquid is prepared from Bacillus paralicheniformis, Paenibacillus jelly-like MSSW03, and Trichoderma pseudokoningii; the active agent is a burdock leaf extract; the dispersant is a modified organic silicon and sodium dodecyl sulfate in a mass ratio of 3:1; the Bacillus paralicheniformis has a strain number of No. CGMCC 1.15832, a preservation date of September 20, 2016, and is purchased from the General Microbiological Center of the China National Committee for the Preservation of Microorganisms; the Paenibacillus jelly-like MSSW03 has a strain number of CCTCC No. M 20231473, the preservation date is August 14, 2023, and it is preserved in the China Center for Type Culture Collection; the Trichoderma pseudokoningii strain number is CCTCC DF 20081027, the preservation date is October 11, 1994, and it was purchased from the China Center for Type Culture Collection.

[0041] The composite microbial fermentation broth is prepared by the following method: (1) Bacillus paralicheniformis, Paenibacillus jelly-like MSSW03, and Trichoderma pseudokoningii were activated and cultured in LB liquid seed medium to obtain a concentration of 2×10 8 cfu / mL~3×10 8 cfu / mL of three seed solutions; (2) inoculating the Bacillus paralicheniformis seed liquid into the fermentation liquid medium I at an inoculum rate of 10%, and fermenting and culturing at 30° C. for 5 days to obtain the Bacillus paralicheniformis fermentation liquid; (3) Inoculate the seed liquid of Paenibacillus jelly-like MSSW03 into the fermentation liquid medium II at an inoculum rate of 8%, and ferment and culture at 28°C for 3 days to obtain the Paenibacillus jelly-like MSSW03 fermentation liquid; (4) inoculating the seed liquid of Trichoderma pseudokoningii into the fermentation liquid medium III at an inoculum rate of 10%, and fermenting and culturing at 28° C. for 3 days to obtain the Trichoderma pseudokoningii fermentation liquid; (5) Mix the fermentation broth of Bacillus paralicheniformis, the fermentation broth of Paenibacillus jelly MSSW03 and the fermentation broth of Trichoderma pseudokoningii in a volume ratio of 1:1:1.

[0042] The LB liquid seed culture medium is composed of: 10 g / L tryptone, 5 g / L yeast extract, and 5 g / L sodium chloride. The pH value is adjusted to 7.0-7.5 and sterilized at about 121° C. for 20 min.

[0043] The fermentation liquid medium I is composed of: 5 g / L yeast powder, 15 g / L starch hydrolyzate, 15 g / L corn steep liquor, 0.05 g / L calcium carbonate, 0.05 g / L magnesium sulfate, 2 g / L potassium dihydrogen phosphate, the pH value is adjusted to 7.0, and sterilized at about 115° C. for 20 min.

[0044] The fermentation liquid medium II is composed of: 10 g / L glucose, 15 g / L starch, 0.5 g / L inositol phospholipids, 0.05 g / L ammonium sulfate, 0.02 g / L ferrous sulfate, and 0.5 g / L potassium dihydrogen phosphate. The pH value is adjusted to 7.0 and sterilized at about 115° C. for 20 min.

[0045] The fermentation liquid medium III is composed of: 10 g / L starch, 5 g / L peptone, 0.05 g / L potassium nitrate, 0.1 g / L dipotassium hydrogen phosphate, 0.05 g / L magnesium sulfate, and 1 g / L sodium chloride. The pH value is adjusted to 7.0 and sterilized at about 115° C. for 20 min.

[0046] The modified silicone preparation method is as follows: cardanol glycidyl ether and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane are mixed in a molar ratio of 1:1 under nitrogen protection, heated to 85° C., refluxed for 1 hour under magnetic stirring, and naturally cooled to room temperature to obtain the modified silicone.

[0047] The burdock leaf extract is prepared by the following method: drying the burdock leaves and then crushing them into 100 meshes to obtain burdock leaf powder; mixing the burdock leaf powder with 50% ethanol solution at a solid-liquid ratio of 1g:30ml, extracting at a pressure of 400MPa for 15min, and centrifuging to obtain a liquid that is the burdock leaf extract.

[0048] A method for preparing the above-mentioned liquid organic fertilizer that can effectively reduce jute damping-off disease comprises the following steps: Step 1: preparing composite microbial fermentation liquid; Step 2: Mix the agricultural and forestry waste and the wastewater from the food processing plant and perform anaerobically fermentation for 5 days to obtain the primary fermentation product; Step 3: sterilize the primary fermentation product and mix it with the composite microbial fermentation liquid, adjust the temperature to 33°C for 12 days, adjust the stirring speed to 180r / min and the ventilation volume to 1.6vvm during fermentation, filter after the fermentation is completed, and take the filtrate to obtain a mixed fermentation liquid; Step 4: prepare active agent and dispersant respectively, then mix them evenly with mixed fermentation liquid and sodium alginate, and pack them into portions.

[0049] Comparative Example 1 A liquid organic fertilizer capable of effectively reducing damping-off disease of jute is prepared from the following raw materials in parts by weight: 120 kg of composite microbial fermentation liquid, 12 kg of sodium alginate, 120 kg of wastewater from a food processing plant, 180 kg of agricultural and forestry wastes, 8 kg of an active agent, and 3 kg of a dispersant; the composite microbial fermentation liquid is prepared from jelly-like Paenibacillus sp. MSSW03 and Trichoderma pseudokoningii; the active agent is a burdock leaf extract; the dispersant is a modified organic silicon and sodium dodecyl sulfate in a mass ratio of 3:1; the jelly-like Paenibacillus sp. MSSW03 has a strain number of CCTCC No.M 20231473, a preservation date of August 14, 2023, and is preserved in China Center for Type Culture Collection; the Trichoderma pseudokoningii has a strain number of CCTCC DF20081027, a preservation date of October 11, 1994, and is purchased from China Center for Type Culture Collection.

[0050] The composite microbial fermentation broth is prepared by the following method: (1) After activating Paenibacillus sp. MSSW03 and Trichoderma pseudokoningii, they were cultured in LB liquid seed medium to obtain a concentration of 2×10 8 cfu / mL~3×10 8 cfu / mL of two seed solutions; (2) Inoculate the seed liquid of Paenibacillus jelly-like MSSW03 into the fermentation liquid medium II at an inoculum rate of 8%, and ferment and culture at 28°C for 3 days to obtain the Paenibacillus jelly-like MSSW03 fermentation liquid; (3) inoculating the seed liquid of Trichoderma pseudokoningii into the fermentation liquid medium III at an inoculum rate of 10%, and fermenting and culturing at 28° C. for 3 days to obtain the Trichoderma pseudokoningii fermentation liquid; (4) Mix the fermentation broth of Bacillus sp. MSSW03 and the fermentation broth of Trichoderma koningii in a volume ratio of 1:1.

[0051] The LB liquid seed culture medium is composed of: 10 g / L tryptone, 5 g / L yeast extract, and 5 g / L sodium chloride. The pH value is adjusted to 7.0-7.5 and sterilized at about 121° C. for 20 min.

[0052] The fermentation liquid medium II is composed of: 10 g / L glucose, 15 g / L starch, 0.5 g / L inositol phospholipids, 0.05 g / L ammonium sulfate, 0.02 g / L ferrous sulfate, and 0.5 g / L potassium dihydrogen phosphate. The pH value is adjusted to 7.0 and sterilized at about 115° C. for 20 min.

[0053] The fermentation liquid medium III is composed of: 10 g / L starch, 5 g / L peptone, 0.05 g / L potassium nitrate, 0.1 g / L dipotassium hydrogen phosphate, 0.05 g / L magnesium sulfate, and 1 g / L sodium chloride. The pH value is adjusted to 7.0 and sterilized at about 115° C. for 20 min.

[0054] The modified silicone preparation method is as follows: cardanol glycidyl ether and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane are mixed in a molar ratio of 1:1 under nitrogen protection, heated to 85° C., refluxed for 1 hour under magnetic stirring, and naturally cooled to room temperature to obtain the modified silicone.

[0055] The burdock leaf extract is prepared by the following method: drying the burdock leaves and then crushing them into 100 meshes to obtain burdock leaf powder; mixing the burdock leaf powder with 50% ethanol solution at a solid-liquid ratio of 1g:30ml, extracting at a pressure of 400MPa for 15min, and centrifuging to obtain a liquid that is the burdock leaf extract.

[0056] A method for preparing the above-mentioned liquid organic fertilizer that can effectively reduce jute damping-off disease comprises the following steps: Step 1: preparing composite microbial fermentation liquid; Step 2: Mix the agricultural and forestry waste and the wastewater from the food processing plant and perform anaerobically fermentation for 5 days to obtain the primary fermentation product; Step 3: sterilize the primary fermentation product and mix it with the composite microbial fermentation liquid, adjust the temperature to 33°C for 12 days, adjust the stirring speed to 180r / min and the ventilation volume to 1.6vvm during fermentation, filter after the fermentation is completed, and take the filtrate to obtain a mixed fermentation liquid; Step 4: prepare active agent and dispersant respectively, then mix them evenly with mixed fermentation liquid and sodium alginate, and pack them into portions.

[0057] This comparative example is basically the same as Example 3, except that the composite microbial fermentation broth does not contain Bacillus paralicheniformis and the corresponding fermentation broth preparation.

[0058] Comparative Example 2 A liquid organic fertilizer capable of effectively reducing damping-off of jute is prepared from the following raw materials in parts by weight: 120 kg of composite microbial fermentation liquid, 12 kg of sodium alginate, 120 kg of waste water from a food processing plant, 180 kg of agricultural and forestry waste, 8 kg of an active agent, and 3 kg of a dispersant; the composite microbial fermentation liquid is prepared from Bacillus paralicheniformis and Trichoderma pseudokoningii; the active agent is a burdock leaf extract; the dispersant is a modified organic silicon and sodium dodecyl sulfate in a mass ratio of 3:1; the Bacillus paralicheniformis has a strain number of No. CGMCC 1.15832, a preservation date of September 20, 2016, and is purchased from the General Microbiology Center of China National Committee for the Collection of Microorganisms; the Trichoderma pseudokoningii has a strain number of CCTCC DF20081027, a preservation date of October 11, 1994, and is purchased from China Center for Type Culture Collection.

[0059] The composite microbial fermentation broth is prepared by the following method: (1) Bacillus paralicheniformis and Trichoderma pseudokoningii were activated and cultured in LB liquid seed medium to obtain a concentration of 2×10 8 cfu / mL~3×10 8 cfu / mL of two seed solutions; (2) inoculating the Bacillus paralicheniformis seed liquid into the fermentation liquid medium I at an inoculum rate of 10%, and fermenting and culturing at 30° C. for 5 days to obtain the Bacillus paralicheniformis fermentation liquid; (3) inoculating the seed liquid of Trichoderma pseudokoningii into the fermentation liquid medium III at an inoculum rate of 10%, and fermenting and culturing at 28° C. for 3 days to obtain the Trichoderma pseudokoningii fermentation liquid; (4) Mix the fermentation broth of Bacillus paralicheniformis and the fermentation broth of Trichoderma pseudokoningii in a volume ratio of 1:1.

[0060] The LB liquid seed culture medium is composed of: 10 g / L tryptone, 5 g / L yeast extract, and 5 g / L sodium chloride. The pH value is adjusted to 7.0-7.5 and sterilized at about 121° C. for 20 min.

[0061] The fermentation liquid medium I is composed of: 5 g / L yeast powder, 15 g / L starch hydrolyzate, 15 g / L corn steep liquor, 0.05 g / L calcium carbonate, 0.05 g / L magnesium sulfate, 2 g / L potassium dihydrogen phosphate, the pH value is adjusted to 7.0, and sterilized at about 115° C. for 20 min.

[0062] The fermentation liquid medium III is composed of: 10 g / L starch, 5 g / L peptone, 0.05 g / L potassium nitrate, 0.1 g / L dipotassium hydrogen phosphate, 0.05 g / L magnesium sulfate, and 1 g / L sodium chloride. The pH value is adjusted to 7.0 and sterilized at about 115° C. for 20 min.

[0063] The modified silicone preparation method is as follows: cardanol glycidyl ether and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane are mixed in a molar ratio of 1:1 under nitrogen protection, heated to 85° C., refluxed for 1 hour under magnetic stirring, and naturally cooled to room temperature to obtain the modified silicone.

[0064] The burdock leaf extract is prepared by the following method: drying the burdock leaves and then crushing them into 100 meshes to obtain burdock leaf powder; mixing the burdock leaf powder with 50% ethanol solution at a solid-liquid ratio of 1g:30ml, extracting at a pressure of 400MPa for 15min, and centrifuging to obtain a liquid that is the burdock leaf extract.

[0065] A method for preparing the above-mentioned liquid organic fertilizer that can effectively reduce jute damping-off disease comprises the following steps: Step 1: preparing composite microbial fermentation liquid; Step 2: Mix the agricultural and forestry waste and the wastewater from the food processing plant and perform anaerobically fermentation for 5 days to obtain the primary fermentation product; Step 3: sterilize the primary fermentation product and mix it with the composite microbial fermentation liquid, adjust the temperature to 33°C for 12 days, adjust the stirring speed to 180r / min and the ventilation volume to 1.6vvm during fermentation, filter after the fermentation is completed, and take the filtrate to obtain a mixed fermentation liquid; Step 4: prepare active agent and dispersant respectively, then mix them evenly with mixed fermentation liquid and sodium alginate, and pack them into portions.

[0066] This comparative example is basically the same as Example 3, except that the composite microbial fermentation broth does not contain the jelly-like Paenibacillus sp. MSSW03 and the corresponding fermentation broth preparation.

[0067] Comparative Example 3 A liquid organic fertilizer capable of effectively reducing damping-off of jute is prepared from the following raw materials in parts by weight: 120 kg of composite microbial fermentation liquid, 12 kg of sodium alginate, 120 kg of wastewater from a food processing plant, 180 kg of agricultural and forestry waste, 8 kg of an active agent, and 3 kg of a dispersant; the composite microbial fermentation liquid is prepared from Bacillus paralicheniformis and Paenibacillus jelly-like MSSW03; the active agent is a burdock leaf extract; the dispersant is a modified organic silicon and sodium dodecyl sulfate in a mass ratio of 3:1; the Bacillus paralicheniformis has a strain number of No. CGMCC 1.15832 and a preservation date of September 20, 2016, purchased from the General Microbiology Center of China National Committee for the Collection of Microorganisms; the Paenibacillus jelly-like MSSW03 has a strain number of CCTCC No. M 20231473 and a preservation date of August 14, 2023, and is preserved in China Center for Type Culture Collection.

[0068] The composite microbial fermentation broth is prepared by the following method: (1) Bacillus paralicheniformis and Paenibacillus jelly-like MSSW03 were activated and cultured in LB liquid seed medium to obtain a concentration of 2×10 8 cfu / mL~3×10 8 cfu / mL of three seed solutions; (2) inoculating the Bacillus paralicheniformis seed liquid into the fermentation liquid medium I at an inoculum rate of 10%, and fermenting and culturing at 30° C. for 5 days to obtain the Bacillus paralicheniformis fermentation liquid; (3) Inoculate the seed liquid of Paenibacillus jelly-like MSSW03 into the fermentation liquid medium II at an inoculum rate of 8%, and ferment and culture at 28°C for 3 days to obtain the Paenibacillus jelly-like MSSW03 fermentation liquid; (4) Mix the fermentation broth of Bacillus paralicheniformis and the fermentation broth of Paenibacillus subtilis MSSW03 in a volume ratio of 1:1.

[0069] The LB liquid seed culture medium is composed of: 10 g / L tryptone, 5 g / L yeast extract, and 5 g / L sodium chloride. The pH value is adjusted to 7.0-7.5 and sterilized at about 121° C. for 20 min.

[0070] The fermentation liquid medium I is composed of: 5 g / L yeast powder, 15 g / L starch hydrolyzate, 15 g / L corn steep liquor, 0.05 g / L calcium carbonate, 0.05 g / L magnesium sulfate, 2 g / L potassium dihydrogen phosphate, the pH value is adjusted to 7.0, and sterilized at about 115° C. for 20 min.

[0071] The fermentation liquid medium II is composed of: 10 g / L glucose, 15 g / L starch, 0.5 g / L inositol phospholipids, 0.05 g / L ammonium sulfate, 0.02 g / L ferrous sulfate, and 0.5 g / L potassium dihydrogen phosphate. The pH value is adjusted to 7.0 and sterilized at about 115° C. for 20 min.

[0072] The modified silicone preparation method is as follows: cardanol glycidyl ether and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane are mixed in a molar ratio of 1:1 under nitrogen protection, heated to 85° C., refluxed for 1 hour under magnetic stirring, and naturally cooled to room temperature to obtain the modified silicone.

[0073] The burdock leaf extract is prepared by the following method: drying the burdock leaves and then crushing them into 100 meshes to obtain burdock leaf powder; mixing the burdock leaf powder with 50% ethanol solution at a solid-liquid ratio of 1g:30ml, extracting at a pressure of 400MPa for 15min, and centrifuging to obtain a liquid that is the burdock leaf extract.

[0074] A method for preparing the above-mentioned liquid organic fertilizer that can effectively reduce jute damping-off disease comprises the following steps: Step 1: preparing composite microbial fermentation liquid; Step 2: Mix the agricultural and forestry waste and the wastewater from the food processing plant and perform anaerobically fermentation for 5 days to obtain the primary fermentation product; Step 3: sterilize the primary fermentation product and mix it with the composite microbial fermentation liquid, adjust the temperature to 33°C for 12 days, adjust the stirring speed to 180r / min and the ventilation volume to 1.6vvm during fermentation, filter after the fermentation is completed, and take the filtrate to obtain a mixed fermentation liquid; Step 4: prepare active agent and dispersant respectively, then mix them evenly with mixed fermentation liquid and sodium alginate, and pack them into portions.

[0075] This comparative example is basically the same as Example 3, except that the composite microbial fermentation broth does not contain Trichoderma pseudokoningii and the corresponding fermentation broth preparation.

[0076] Comparative Example 4 This comparative example is basically the same as Example 3, except that the three types of composite microbial fermentation broth are mixed in a volume ratio of 1:2:1.

[0077] Comparative Example 5 This comparative example is basically the same as Example 3, except that it does not contain an active agent and a preparation method of the active agent.

[0078] Comparative Example 6 This comparative example is basically the same as Example 3, except that it does not contain a dispersant and its preparation method.

[0079] Comparative Example 7 This comparative example is basically the same as Example 3, except that the dispersant is modified silicone.

[0080] Comparative Example 8 This comparative example is basically the same as Example 3, except that the dispersant is sodium dodecyl sulfate.

[0081] Application Examples Jute field planting experiment An agricultural planting base in Tiemengang Township, Macheng City, Hubei Province was selected for planting experiments. The test variety was Huangju 179 (provided by Fujian Agriculture and Forestry University), and the test soil was yellow-brown soil. The basic information of the topsoil (0-20 cm) before the start of the experiment was as follows: total nitrogen 1.12 g / kg, total phosphorus 0.46 g / kg, total potassium 5.5 g / kg, alkaline nitrogen 41.9 mg / kg, available phosphorus 11.9 mg / kg, available potassium 76.9 mg / kg, organic matter 5.51 g / kg, bulk density 1.12 g / cm3 , pH = 6.95. A total of 11 treatment groups were set up in the experiment, namely the liquid organic fertilizer treatment group of Example 3, the liquid organic fertilizer treatment groups of Comparative Examples 1-8, the commercially available control group and the blank control group. Each treatment was repeated 3 times, with a total of 33 plots, each with an area of ​​50m 2 , the planting density is 300,000 plants / hm 2 . Before sowing jute, each plot applied 10 kg / mu of ordinary commercial compound fertilizer (N-P2O5-K2O=15-15-15). The fertilizer application method was to spread the fertilizer on the surface once as base fertilizer, and the amount of fertilizer applied in each plot was consistent. After the seedlings were all grown, each treatment group was applied with different liquid organic fertilizers, with a usage of 5 kg / mu. The liquid fertilizers can be sprayed directly or diluted with clean water before spraying; the commercial control group was topdressed with an equal amount of compound microbial fertilizer purchased from Anyang Zhongsheng Fertilizer Technology Co., Ltd. (effective live bacteria count ≥ 20 million / g, N+P2O5+K2O=8.0%, organic matter ≥ 20.0%), and flushed with water; the blank control group was sprayed with an equal amount of clean water. The number of plants with damping-off disease in each treatment group during the entire growth period was counted, and the damping-off disease incidence rate of each treatment group was calculated. The incidence rate of the three treatment groups was taken as the average. Incidence rate (%) = number of diseased plants / total number of plants surveyed × 100. The specific statistical results are shown in Table 1 below.

[0082] Table 1 Statistical results of the incidence of jute damping-off disease From the results in Table 1 above, it can be seen that compared with the blank control group, the incidence of jute damping-off disease was significantly reduced after using the liquid organic fertilizer prepared in Example 3 of the present invention, and compared with the commercially available control group, the incidence of jute damping-off disease was also significantly reduced after using the liquid organic fertilizer prepared in Example 3 of the present invention, which shows that the liquid organic fertilizer prepared by the present invention can effectively reduce the incidence of jute damping-off disease. The data in Comparative Examples 1-8 show that by changing any raw material composition and ratio in the present invention, the effect of preventing and controlling jute damping-off disease is weakened or disappears accordingly.

[0083] During the period of jute vigor, 10 fresh middle and upper functional leaves were randomly selected from each treatment group, washed and cleaned of impurities on the surface of the tissue, cut into strips, weighed 0.5g and placed in a 5 mL centrifuge tube, added 5 mL of 95% anhydrous ethanol extract, placed in a dark place and soaked until the leaves turned completely white, took the supernatant and measured the absorbance values ​​at 665 nm, 649 nm and 470 nm with an enzyme marker, and calculated the total chlorophyll content of each treatment group according to the following formula. The specific results are shown in Table 2.

[0084] The calculation formula is as follows: C a =13.95A 665 -6.88A 649 C b =24.96A 649 -7.32A 665 C x·c =(1000A 470 -2.05C a -114.8C b ) / 245 C T =C a +C b In the formula, C a is the chlorophyll a content (mg / g); C b is the chlorophyll b content (mg / g); C x·c is the carotenoid content (mg / g); C T is the total amount of chlorophyll (mg / g); A 665 , A 649 , A 470 They are the absorbance values ​​at wavelengths of 665 nm, 649 nm, and 470 nm respectively.

[0085] Table 2 Total chlorophyll content of jute leaves in different treatment groups The jute was harvested at the mature stage and the jute dry bark yield (kg / mu) of each treatment group was counted. The specific statistical results are shown in Table 3. Compared with the blank control group, the total chlorophyll content of the embodiment of the present invention was significantly increased, which shows that the liquid organic fertilizer of the present invention has a promoting effect on photosynthesis in the growth of jute after application. The enhancement of photosynthesis can increase the photosynthetic rate of jute leaves, increase the accumulation of photosynthetic products, provide necessary energy and nutrients for jute, and also promote the growth and development of jute, and increase the yield of fresh stem bark of jute.

[0086] Table 3 Effects of different treatment groups on jute yield The present invention also determined the activity of defense enzymes in the vigorous growth period leaves of jute in different treatment groups. The specific operation method is: weigh 0.1 g of jute leaves and add 3 ml of 0.05 mol / L PBS (pH 7.8), grind and homogenize in an ice bath, then add 2 mL of PBS to rinse the mortar, mix well, pour into a centrifuge tube and centrifuge at 10000r / min at 4°C for 20 min. The supernatant is the crude enzyme solution, which can be used for enzyme activity determination. The contents of SOD, CAT and POD in the crude enzyme extract were determined respectively, among which SOD was determined by the nitroblue tetrazolium photoreduction method; CAT was determined by the ultraviolet absorption method, and POD was determined by the guaiacol method. The specific results are as follows: Figure 1-3 shown.

[0087] The antioxidant system can remove excess reactive oxygen and improve physiological activity. It is an important system for plants to self-regulate and improve resistance under adversity. Figure 1-3 It can be seen that after the liquid organic fertilizer prepared in the embodiment of the present invention is used, the superoxide dismutase SOD, catalase CAT and peroxidase POD enzyme activities in the jute leaves are significantly higher than those in the comparative example, the blank control example and the commercially available control example, which indicates that after the liquid organic fertilizer of the present invention is used, the defensive enzyme activity in the antioxidant system of the jute leaves is improved, the stress resistance of the jute itself is improved, various stress conditions can be better coped with, the infection of pests and diseases is reduced, and the damping-off disease prone to occur in jute planting also has a good prevention and control effect.

[0088] It should be noted that the above embodiments are only partial embodiments of the preferred methods of implementing the present invention, rather than all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

Claims

1. A liquid organic fertilizer that can effectively reduce jute damping-off disease, characterized in that: The invention is prepared from the following raw materials in parts by weight: 100-150 parts of composite microbial fermentation liquid, 10-15 parts of sodium alginate, 100-130 parts of waste water from a food processing plant, 150-200 parts of agricultural and forestry waste, 5-10 parts of an active agent, and 1-5 parts of a dispersant; the composite microbial fermentation liquid is prepared from Bacillus paralicheniformis, Paenibacillus jelly-like MSSW03, and Trichoderma pseudokoningii; the active agent is burdock leaf extract; the dispersant is a modified organic silicon and sodium dodecyl sulfate in a mass ratio of 3:1; the Bacillus paralicheniformis has a strain number of No. CGMCC 1.15832, a preservation date of September 20, 2016, and is purchased from the General Microbiological Center of the China National Committee for the Preservation and Administration of Microbiological Cultures; the Paenibacillus jelly-like MSSW03 has a strain number of CCTCC No. M 20231473, the preservation date is August 14, 2023, and it is preserved in the China Center for Type Culture Collection; the Trichoderma pseudokoningii strain number is CCTCCDF 20081027, the preservation date is October 11, 1994, and it was purchased from the China Center for Type Culture Collection.

2. The liquid organic fertilizer that can effectively reduce jute blight according to claim 1, characterized in that: The composite microbial fermentation broth is prepared by the following method: (1) Bacillus paralicheniformis, Paenibacillus jelly-like MSSW03, and Trichoderma pseudokoningii were activated and cultured in LB liquid seed medium to obtain a concentration of 2×10 8 cfu / mL~3×10 8 cfu / mL of three seed solutions; (2) inoculating the Bacillus paralicheniformis seed liquid into the fermentation liquid medium I at an inoculum rate of 10%, and fermenting and culturing at 30° C. for 5 days to obtain the Bacillus paralicheniformis fermentation liquid; (3) Inoculate the seed liquid of Paenibacillus jelly-like MSSW03 into the fermentation liquid medium II at an inoculum rate of 8%, and ferment and culture at 28°C for 3 days to obtain the Paenibacillus jelly-like MSSW03 fermentation liquid; (4) inoculating the seed liquid of Trichoderma pseudokoningii into the fermentation liquid medium III at an inoculum rate of 10%, and fermenting and culturing at 28° C. for 3 days to obtain the Trichoderma pseudokoningii fermentation liquid; (5) Mix the fermentation broth of Bacillus paralicheniformis, the fermentation broth of Paenibacillus jelly-like MSSW03 and the fermentation broth of Trichoderma pseudokoningii in a volume ratio of 1:1:

1.

3. The liquid organic fertilizer that can effectively reduce jute blight according to claim 2, characterized in that: The fermentation liquid medium I is composed of: 5 g / L yeast powder, 15 g / L starch hydrolyzate, 15 g / L corn steep liquor, 0.05 g / L calcium carbonate, 0.05 g / L magnesium sulfate, 2 g / L potassium dihydrogen phosphate, the pH value is adjusted to 7.0, and sterilized at about 115° C. for 20 min.

4. The liquid organic fertilizer that can effectively reduce jute blight according to claim 2, characterized in that: The fermentation liquid medium II is composed of: 10 g / L glucose, 15 g / L starch, 0.5 g / L inositol phospholipids, 0.05 g / L ammonium sulfate, 0.02 g / L ferrous sulfate, and 0.5 g / L potassium dihydrogen phosphate. The pH value is adjusted to 7.0 and sterilized at about 115° C. for 20 min.

5. The liquid organic fertilizer that can effectively reduce jute blight according to claim 2, characterized in that: The fermentation liquid medium III is composed of: 10 g / L starch, 5 g / L peptone, 0.05 g / L potassium nitrate, 0.1 g / L dipotassium hydrogen phosphate, 0.05 g / L magnesium sulfate, and 1 g / L sodium chloride. The pH value is adjusted to 7.0 and sterilized at about 115° C. for 20 min.

6. The liquid organic fertilizer that can effectively reduce jute blight according to claim 1, characterized in that: The modified silicone preparation method is as follows: cardanol glycidyl ether and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane are mixed in a molar ratio of 1:1 under nitrogen protection, heated to 80-85° C., refluxed for 1-2 hours under magnetic stirring, and naturally cooled to room temperature to obtain the modified silicone.

7. The liquid organic fertilizer that can effectively reduce jute blight according to claim 1, characterized in that: The burdock leaf extract is prepared by the following method: drying the burdock leaves and then crushing them into 100 meshes to obtain burdock leaf powder; mixing the burdock leaf powder with 50% ethanol solution at a solid-liquid ratio of 1g:30ml, extracting at a pressure of 400MPaaa for 15min, and centrifuging to obtain a liquid that is the burdock leaf extract.

8. A method for preparing a liquid organic fertilizer capable of effectively reducing jute damping-off disease according to any one of claims 1 to 7, characterized in that: It includes the following steps: Step 1: preparing composite microbial fermentation liquid; Step 2: Mix the agricultural and forestry waste and the wastewater from the food processing plant and perform anaerobically fermentation for 5 days to obtain the primary fermentation product; Step 3: Sterilize the primary fermentation product and mix it with the composite microbial fermentation liquid, adjust the temperature to 30-35°C for fermentation culture for 10-12 days, adjust the stirring speed to 150-200r / min and the ventilation volume to 1.5-2.0vvm during fermentation, filter after fermentation, and take the filtrate to obtain a mixed fermentation liquid; Step 4: prepare active agent and dispersant respectively, then mix them evenly with mixed fermentation liquid and sodium alginate, and pack them into portions.

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