Method for preparing agricultural enzyme by using wild plant humulus scandens and application of agricultural enzyme

Abstract

The invention relates to a method for preparing an agricultural enzyme by using wild plant humulus scandens and application of the agricultural enzyme. According to the method, fresh scandent hop, brown sugar and tap water are used as raw materials and mixed according to the mass ratio of 1: 5: 20, aerobic fermentation is conducted for 6-8 weeks, anaerobic fermentation is conducted for 2-3 weeks, and finally the agricultural enzyme is obtained through filtration. The prepared enzyme is brown yellow-brown yellow in color, has the pH value of 3.0-5.0, is rich in organic matters, organic acids, amino acids and plant hormones, and meets the regulation of the national standard QB / T5323-2018 plant enzyme. The preparation method is simple, low in cost and suitable for vegetable seedling raising and topdressing, and the fruit and vegetable seedling strengthening index and the leaf vegetable yield and quality can be remarkably improved. After being diluted by 100-500 times, the fertilizer can be used for irrigating and applying, and can replace part of chemical fertilizers, so that the development of ecological cycle agriculture is promoted. The invention provides a new way for resource utilization and harmless treatment of humulus japonicus, and has important ecological and economic significance.

Description

Technical Field

[0001] The present invention relates to the technical field of enzyme processing, and in particular to a method for preparing agricultural enzymes by utilizing wild plant Humulus japonicus and application thereof. Background Art

[0002] Agricultural plant enzymes are products containing specific biologically active ingredients that are made from plants through microbial fermentation. They are widely used in the fields of planting, breeding and soil improvement, and are an important part of ecological circular agriculture. In recent years, with the global emphasis on sustainable agriculture and environmental protection, agricultural plant enzymes have gradually become a research hotspot in the agricultural field due to their positive role in promoting plant growth, improving crop quality, improving soil ecology, and reducing environmental pollution.

[0003] Agricultural plant enzymes can not only replace part of chemical fertilizers and reduce agricultural production’s dependence on chemical fertilizers, but also improve the structure of soil microbial communities, enhance soil fertility, and promote the sustainable development of agricultural production.

[0004] At present, the main raw materials for preparing agricultural plant enzymes include fresh fruits and vegetables, waste vegetables and kitchen waste. For example, defective fruits or peels such as apples, grapes, and citrus, peppers, tomato straw or vegetable scraps, etc. Although these raw materials are widely available, their utilization efficiency is limited and there is a problem of seasonal supply shortage. In addition, the existing technology has little research on the use of wild plant resources for agricultural plant enzymes, especially the development and utilization of wild plants with rich biomass and unique chemical composition has not been fully explored.

[0005] Humulus scandens is a perennial herbaceous vine of the genus Humulus in the Moraceae family, widely distributed in provinces and regions in the north and south of my country (except Xinjiang and Qinghai). Humulus scandens is resistant to barrenness, cold and drought, and has a large biomass. It often grows in ditches, wastelands, ruins and forest edges. Although Humulus scandens is regarded as an agricultural pest, its whole grass contains rich organic matter (such as crude protein, crude fat) and mineral nutrients (such as calcium, phosphorus, iron, manganese, zinc, copper, etc.), as well as unique secondary metabolites such as flavonoids, alkaloids, luteolin, apigenin, etc. These characteristics give Humulus scandens great potential for development into agricultural enzymes.

[0006] At present, the development and utilization of Humulus japonicus is mainly concentrated in the fields of livestock breeding, medicinal and industrial production, but its application in agriculture has not been fully explored. The present invention aims to provide a method for preparing agricultural enzymes using fresh Humulus japonicus, and explore its application in the planting industry, to provide a new way for the resource utilization and harmless treatment of Humulus japonicus, and at the same time to promote the development of ecological circular agriculture. By converting Humulus japonicus into agricultural enzymes, not only can this wild plant resource be effectively utilized, but also a low-cost, high-efficiency organic nutrient solution can be provided for agricultural production, which has important ecological and economic significance. Summary of the invention

[0007] In view of the above background, the object of the present invention is to provide a method for preparing a wild plant agricultural enzyme and its application. The method uses fresh wild Humulus as the main raw material, prepares an agricultural enzyme rich in organic nutrients and mineral nutrients through a simple fermentation process, and applies it to the planting industry to improve crop quality and yield. The agricultural plant enzyme produced by the present invention has rich organic nutrients and mineral nutrients in variety and high content, and has obvious effects in seedling cultivation and leafy vegetable production, which can be comparable to the use effect of 1 / 2 Hogland nutrient solution. And the enzyme is applied in the planting industry, opening up a way for the resource utilization and harmless treatment of Humulus.

[0008] To achieve the above object, the present invention provides a method for preparing agricultural enzymes using fresh Humulus japonicus, and the specific steps are as follows:

[0009] S1. Raw material pretreatment: Collect fresh wild Humulus, chop or crush into 2 cm pieces, and set aside.

[0010] S2. Barreling: Add sugar, humulum and water in a mass ratio of 1:5:20 into a fermentation barrel, mix well, cover and seal, and ferment naturally at room temperature of about 25°C in the dark.

[0011] S3. Aerobic fermentation: 6 to 8 weeks, open the lid and stir every 3 to 5 days.

[0012] S4. Anaerobic fermentation: Filter once with clean gauze and seal the filtrate completely for anaerobic fermentation for 2 to 3 weeks. Alternatively, seal the filtrate directly without filtering for anaerobic fermentation.

[0013] S5. Filtration: After the final fermentation is completed, filter with clean gauze and the filtrate is the agricultural enzyme.

[0014] Furthermore, the fermentation time of the early aerobic fermentation is 6 to 8 weeks, and the pH value and EC value can be monitored every time the fermentation is stirred; the time of the later anaerobic fermentation is 2 to 3 weeks, and the total fermentation time is not less than 8 weeks.

[0015] Furthermore, the raw sugar is selected from brown sugar, white sugar or honey; the water is selected from distilled water, purified water or other clean water sources that meet the drinking water standards; the raw material of Humulus japonicus is preferably fresh wild Humulus japonicus stems and leaves from the vine extension stage to the blooming stage; the raw materials are preferably brown sugar, fresh Humulus japonicus and tap water.

[0016] The agricultural enzyme prepared by the present invention is brown-yellow to brown-yellow in color, has no obvious odor, has a pH of 3.0-5.0, a conductivity of 6.0-10.0 mS / cm, organic matter ≥5 g / L, organic acid ≥10 g / L, 20 kinds of standard amino acid contents ≥0.5 g / L, contains a variety of plant hormones or hormone-like substances, and meets the provisions of the national standard "QB / T5323-2018 Plant Enzymes" for agricultural plant enzymes.

[0017] Furthermore, the plant hormones or quasi-hormones in the agricultural enzyme are mainly indoleacetic acid (IAA), jasmonic acid (JA) and salicylic acid (SA).

[0018] Furthermore, the agricultural enzyme is rich in organic acids, and the organic acids are mainly acetic acid, butyric acid and lactic acid.

[0019] Furthermore, the agricultural enzyme is rich in various amino acids, and the amino acids are mainly alanine, tyrosine and methionine.

[0020] Furthermore, the prepared enzyme needs to be stored in a cool place to avoid long-term exposure to sunlight.

[0021] The present invention also provides an application of the above agricultural enzyme, specifically:

[0022] The agricultural enzyme is diluted 100 to 500 times with water and then applied to crops through irrigation.

[0023] Furthermore, the recommended usage concentration of the agricultural enzyme in vegetable seedling cultivation is 400 times dilution, and that for solanaceous fruits is 100 times dilution, and the irrigation frequency is once every 3 to 7 days.

[0024] Furthermore, the recommended concentration of the agricultural enzyme in leafy vegetable cultivation is 500 times diluted solution, and the irrigation frequency is once every 3 to 7 days.

[0025] The present invention has at least the following beneficial effects:

[0026] 1. Humulus has the characteristics of being resistant to barrenness, cold and drought, and having a large biomass, and can be used for desertification control and vegetation restoration in abandoned mining areas. The present invention provides a new available way for the Humulus produced for ecological improvement, which has important ecological significance.

[0027] 2. The invention has simple preparation and low production cost, and provides a new way for the resource utilization and harmless treatment of Humulus, so that the weeds in the field are converted into organic nutrient solution, so that the nutrients are returned to the field to improve soil fertility and realize nutrient circulation.

[0028] 3. This application also discloses a method for using agricultural enzymes. The prepared agricultural enzymes can be used for vegetable seedling cultivation and topdressing, etc., which can improve the fruit and vegetable seedling index and increase the yield and quality of leafy vegetables, with significant effects. This invention achieves organic fertilizer substitution and helps reduce the use of chemical fertilizers.

[0029] 4. This application broadens the ideas for green or organic production of vegetables and provides a reference for the development and utilization of other wild plant resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a graph showing the mineral nutrition, organic acid, hormone and amino acid content of agricultural enzymes according to an embodiment of the present invention.

[0031] Figure 2 This is a graph showing the effects of different concentrations of agricultural enzymes on the growth of tomato seedlings in an example of the present invention.

[0032] Figure 3 This is a graph showing the effects of different concentrations of agricultural enzymes on the growth of cucumber seedlings in an example of the present invention.

[0033] Figure 4 This is a graph showing the effect of a 500-fold dilution of the agricultural enzyme of an example of the present invention on the yield and quality of Chinese cabbage. DETAILED DESCRIPTION

[0034] In order to make the above-mentioned purpose, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below in conjunction with the embodiments and drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific implementation disclosed below.

[0035] Example:

[0036] This example describes in detail a method for preparing agricultural enzymes using wild plant Humulus japonicus and its application. The specific steps are as follows:

[0037] Raw material preparation

[0038] 1. Fresh Humulus: Carefully collect wild Humulus in the mature stage of farmland. In order to ensure the consistency and stability of the raw materials, during the collection process, select Humulus plants with good growth and no obvious diseases and insect pests. After collection, use a clean kitchen knife to cut the Humulus into pieces with a uniform length of 2 cm. This length setting is conducive to the full contact between microorganisms and Humulus in the subsequent fermentation process, promoting the release and transformation of effective ingredients.

[0039] 2. Sugar: Use brown sugar produced by Zhengzhou Shuangniu Seasoning Food Co., Ltd. This brand of brown sugar has stable quality and pure ingredients. It can provide sufficient carbon source for the fermentation process, which is conducive to the growth and metabolism of microorganisms, and thus has a positive impact on the quality of agricultural enzymes.

[0040] 3. Water: Tap water that meets national drinking water standards is used as the solvent. Its water quality is stable and its source is wide, which can meet the needs of large-scale production.

[0041] Raw material weighing and barrel mixing

[0042] Use an accurate electronic scale to accurately weigh brown sugar, humus and tap water in a mass ratio of 1:5:20. Load the weighed brown sugar, humus segments and tap water into a specific plastic fermentation barrel in turn. The plastic fermentation barrel used in this embodiment has a capacity of 10L and is made of food-grade PC material. This material has good chemical stability and hygienic properties, meets the safety standards for food contact materials, and can ensure that the raw materials and fermentation products will not be contaminated during the fermentation process. The fermentation barrel is equipped with a sealing cover and a liquid collection faucet. The sealing cover can effectively prevent external impurities from entering the fermentation barrel while maintaining the relatively closed environment required for fermentation; the liquid collection faucet is convenient for accurate and convenient acquisition of the fermentation product after the fermentation is completed. After the raw materials are loaded into the fermentation barrel, stir them thoroughly to ensure that the brown sugar is completely dissolved and the humus segments are evenly dispersed in the solution, creating good starting conditions for subsequent fermentation.

[0043] Fermentation process

[0044] Aerobic fermentation stage: After the fermentation barrel containing the mixed raw materials is sealed with a lid, it is placed in an environment with a room temperature of about 25°C for fermentation. In order to simulate the relatively stable light conditions in the natural environment, the fermentation barrel is shaded with black plastic film. In the early stage of fermentation, the sealed lid is opened and stirred every 3-5 days. The purpose of this operation is to provide sufficient oxygen for the fermentation system, promote the growth and metabolism of aerobic microorganisms, and accelerate the initial decomposition and fermentation process of the raw materials. The aerobic fermentation stage lasts about 6-8 weeks, during which time the microbial community in the fermentation system is gradually established and active, and the raw materials begin to undergo a series of complex biochemical reactions.

[0045] Anaerobic fermentation stage: After aerobic fermentation, the fermentation barrel is completely sealed and enters the anaerobic fermentation stage. This stage lasts for 2-3 weeks. In an anaerobic environment, anaerobic microorganisms play a leading role and further transform the fermentation substrate to generate a variety of metabolites that are beneficial to the growth of crops. Through the continuous fermentation of these two stages, the metabolic characteristics of different types of microorganisms can be fully utilized to maximize the enrichment of the effective ingredients in agricultural enzymes.

[0046] Fermentation product processing and detection

[0047] After the fermentation is completed, the fermentation liquid is filtered with clean gauze to remove solid impurities such as the incompletely decomposed Humulus residue. The filtrate obtained is the target product - agricultural enzyme. In order to fully understand the quality and characteristics of the prepared agricultural enzyme, multiple indicators are measured. The specific measurement results are as follows:

[0048] Appearance and smell: The prepared agricultural enzyme is brown-yellow in color and has a unique odor characteristic, which combines the aroma of acetic acid, ester and wine, and has no obvious odor. This odor characteristic not only reflects the smooth progress of the fermentation process, but also suggests that the agricultural enzyme contains rich volatile organic compounds, which may have a positive impact on the growth and quality of crops.

[0049] Physical and chemical indicators: The final pH value of the agricultural enzyme was 3.84, and the electrical conductivity (EC) was 6.89mS / cm. These values ​​reflect the acidity and alkalinity and ion concentration of the agricultural enzyme. The appropriate pH value and conductivity are conducive to its application in agricultural production, such as better adaptation to crops and soil environment during irrigation or foliar spraying. At the same time, the agricultural enzyme prepared in this embodiment is rich in organic matter, and the organic matter content is 11.25g / L after testing.

[0050] Organic acid composition: The organic acid composition in agricultural enzymes was analyzed, and the specific contents were as follows in terms of short-chain fatty acids: acetic acid 9013.96 mg / L, propionic acid 437.02 mg / L, isobutyric acid 409.09 mg / L, butyric acid 6635.94 mg / L, isovaleric acid 53.27 mg / L, valeric acid 96.22 mg / L, caproic acid 351.45 mg / L, lactic acid 488.23 mg / L, totaling 17485.18 mg / L (about 17.5 g / L). Among them, acetic acid, butyric acid and lactic acid ranked the top three (relevant data are attached). Figure 1 These organic acids have many functions in agricultural production, such as regulating soil pH, promoting soil microbial activity, etc., which helps to improve the soil environment and enhance the growth quality of crops.

[0051] Amino acid composition: The agricultural enzyme is rich in 20 kinds of standard amino acids, with a cumulative content of 606.16 mg / L (about 0.6 g / L). Among these amino acids, alanine, tyrosine and methionine are the main ones, with contents exceeding 100 mg / L, namely 105.65 mg / L, 177.86 mg / L and 109.74 mg / L respectively (see attached Figure 1 Amino acids are the basic units of protein and play a vital role in the growth and development, metabolism and stress resistance of crops.

[0052] Plant hormone or hormone-like ingredients: In addition, the agricultural enzyme is rich in plant hormones or hormone-like ingredients, mainly indoleacetic acid (IAA), jasmonic acid (JA) and salicylic acid (SA), which are 190.95μg / L, 122.56μg / L and 167.22μg / L respectively (as shown in the attached Figure 1 These plant hormones or hormone-like substances can regulate the growth, development and physiological processes of crops, such as promoting rooting, flowering and fruiting, and enhancing the resistance of crops to pests and diseases and adversity.

[0053] This embodiment successfully prepared wild plant agricultural enzymes with specific quality and rich nutrients through specific raw material selection, precise operating steps and strict fermentation condition control, laying a solid foundation for its wide application in the agricultural field.

[0054] Application of agricultural enzymes

[0055] Application 1: Tomato seedling cultivation

[0056] Experimental setup

[0057] 1. Seedling medium: Use conventional seedling medium.

[0058] 2. Seed treatment: After germinating the tomato seeds, sow them in a 50-hole plug tray.

[0059] 3. Treatment group setting: The experiment set up multiple treatment groups, and the treatments were 50 times, 100 times, 200 times, 300 times, 400 times, and 500 times diluted Humulus enzyme.

[0060] 4. Control group setting:

[0061] Negative control: Use clean water as negative control.

[0062] Positive control: 1 / 2 Hoagland nutrient solution (abbreviated as 1 / 2HG nutrient solution) was used as the positive control.

[0063] Process

[0064] 1. Treatment start time: After the tomato cotyledons flattened, start the corresponding treatment for each treatment group.

[0065] 2. Treatment frequency: Treat every 3-7 days, depending on the moisture status of the substrate.

[0066] Data measurement and analysis

[0067] 1. Determination time: After 4 weeks, sampling was carried out when the seedlings had 3 leaves and 1 heart.

[0068] 2. Determination indicators:

[0069] Plant Height: Measure the plant height of the seedlings.

[0070] Stem diameter: Measure the stem diameter of the seedlings.

[0071] Whole plant dry weight: Measure the whole plant dry weight of the seedlings.

[0072] 3. Calculation of seedling index: The seedling index is calculated based on plant height, stem diameter and whole plant dry weight.

[0073] Test results

[0074] 1. Data analysis: Through the collation and analysis of the test data (relevant data can be found in the attached Figure 2 ) It can be seen that the seedling index is the highest when the dilution is 100 times.

[0075] 2. Effect comparison: At this time, the effect is equivalent to that of 1 / 2HG nutrient solution.

[0076] 3. Conclusion: This shows that at this dilution multiple, Humulus enzyme has a significant promoting effect on the growth of tomato seedlings and can cultivate strong tomato seedlings.

[0077] Through the above detailed experimental settings, processing procedures, data measurement and analysis, this application example fully demonstrated the application effect of Humulus enzyme in tomato seedling cultivation, and provided a scientific basis for its promotion in agricultural production.

[0078] Application 2: Cucumber seedling cultivation

[0079] Experimental setup

[0080] 1. Seedling medium: Use conventional seedling medium.

[0081] 2. Seed treatment: After germinating the cucumber seeds, sow them in 50-hole plug trays.

[0082] 3. Treatment group setting: The experiment set up multiple treatment groups, and the treatments were 50 times, 100 times, 200 times, 300 times, 400 times, and 500 times diluted Humulus enzyme.

[0083] 4. Control group setting:

[0084] Negative control: Use clean water as negative control.

[0085] Positive control: 1 / 2 Hoagland nutrient solution (abbreviated as 1 / 2HG nutrient solution) was used as the positive control.

[0086] Process

[0087] 1. Treatment start time: After the cucumber cotyledons are flattened, start the corresponding treatment for each treatment group.

[0088] 2. Treatment frequency: Treat every 3-7 days, depending on the moisture status of the substrate.

[0089] Data measurement and analysis

[0090] 1. Determination time: After 3 weeks, sampling was carried out when the seedlings had 3 leaves and 1 heart.

[0091] 2. Determination indicators:

[0092] Plant Height: Measure the plant height of the seedlings.

[0093] Stem diameter: Measure the stem diameter of the seedlings.

[0094] Whole plant dry weight: Measure the whole plant dry weight of the seedlings.

[0095] 3. Calculation of seedling index: The seedling index is calculated based on plant height, stem diameter and whole plant dry weight.

[0096] Test results

[0097] 1. Data analysis: Through the collation and analysis of the test data (relevant data can be found in the attached Figure 3 ) It can be seen that when the dilution is 400 times, the seedling index of cucumber seedlings is the highest.

[0098] 2. Effect comparison: At this time, the effect is equivalent to that of 1 / 2HG nutrient solution.

[0099] 3. Conclusion: This shows that at this dilution multiple, Humulus enzyme has a significant promoting effect on the growth of cucumber seedlings and can cultivate strong cucumber seedlings.

[0100] Through the above-mentioned detailed experimental settings, processing procedures, data measurement and analysis, this application example fully demonstrated the application effect of Humulus enzyme in cucumber seedling cultivation, and provided a scientific basis for its promotion in agricultural production.

[0101] Application 3: Soilless cultivation of Chinese cabbage

[0102] Experimental setup

[0103] 1. Seedling medium: Use conventional seedling medium.

[0104] 2. Seed treatment: After germinating the Chinese cabbage seeds, sow them in 50-hole plug trays.

[0105] 3. Transplantation: Transplant the seedlings when they have 2 leaves and 1 heart. During the transplanting process, select seedlings with uniform growth and robustness and transplant them into 7cm×7cm seedling pots.

[0106] Process

[0107] 1. Acclimatization: After transplanting the seedlings, let them grow slowly for 3 days.

[0108] 2. Treatment start time: Treatment begins after the seedlings have finished acclimatizing.

[0109] 3. Treatment frequency: once every 3-7 days.

[0110] 4. Treatment fluid dosage:

[0111] In the early stage, 100 mL of treatment solution was applied to each root.

[0112] When the seedlings grow to 4 leaves and 1 heart, apply 200 mL to each root.

[0113] 5. Treatment group settings:

[0114] Treatment group: Root application test was conducted using 500-fold diluted Humulus enzyme.

[0115] Control group: 1 / 2 Hoagland nutrient solution was used as a control.

[0116] 6. Number of treatments: The whole experiment was treated 3 times in total.

[0117] Data measurement and analysis

[0118] 1. Determination indicators:

[0119] Yield: Determine the yield of each white vegetable.

[0120] Quality indicators: Determination of flavonoids, anthocyanins, Vc, soluble sugar and cellulose content.

[0121] 2. Data analysis: Through statistical analysis of the test results (relevant data can be found in the attached Figure 4 ) It can be seen that:

[0122] Yield: The single-plant yield of white taro treated with 500-fold enzyme dilution was significantly higher than that treated with 1 / 2 Hoagland nutrient solution.

[0123] Quality: In terms of quality indicators, the contents of flavonoids, anthocyanins, Vc, soluble sugars and cellulose in enzyme-treated samples were slightly higher than those in samples treated with 1 / 2 Hoagland nutrient solution.

[0124] Test results

[0125] 1. Yield improvement: The yield of a single plant of Chinese cabbage treated with 500 times diluted enzyme was significantly higher than that treated with 1 / 2 Hoagland nutrient solution, indicating that Humulus enzyme has a significant effect on improving the yield of Chinese cabbage.

[0126] 2. Quality improvement: The content of flavonoids, anthocyanins, Vc, soluble sugar and cellulose in the enzyme-treated pakchoy were slightly higher than those in the 1 / 2 Hoagland nutrient solution treatment, indicating that Humulus enzyme can improve the quality of pakchoy to a certain extent.

[0127] Through the above detailed experimental settings, processing procedures, data measurement and analysis, this application example fully demonstrated the application effect of Humulus enzyme in soilless cultivation of Chinese cabbage, and provided a scientific basis for its promotion in agricultural production.

[0128] In summary, this embodiment successfully prepared a wild plant agricultural enzyme with specific quality and rich nutrients through specific raw material selection, precise operation steps and strict fermentation condition control. The agricultural enzyme has shown good effects in agricultural production applications such as tomato seedling cultivation, cucumber seedling cultivation and soilless cultivation of Chinese cabbage, providing a strong basis for its wide promotion and application in the agricultural field.

[0129] The patent protection scope of the present invention shall be based on its claims. All equivalent structural changes made using the contents of the description and drawings of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing agricultural enzymes using wild plant Humulus japonicus, characterized in that: The following steps are involved: Step 1: Raw material preparation: The raw materials include sugar, wild plants and water. Crush the wild plants into 2 cm pieces; Step 2: Mixing raw materials: Add sugar, wild plants and water into the fermentation barrel in a mass ratio of 1:5:20 (w / w / w) and mix thoroughly; Step 3: Aerobic fermentation: Cover and seal, and ferment naturally at 25°C in a dark place. The early stage of fermentation is aerobic fermentation. During this period, open the cover and stir once every 3 to 5 days for 6 to 8 weeks. Step 4: Anaerobic fermentation: Completely seal and perform anaerobic fermentation for 2 to 3 weeks, with a total fermentation time of no less than 8 weeks; Step 5: Filtration and storage: Filter with clean gauze to obtain agricultural enzymes. The agricultural enzymes comply with the national standard "QB / T5323-2018 Plant Enzymes" and need to be stored in a cool place away from light.

2. The method for preparing agricultural enzymes using wild plant Humulus japonicus according to claim 1, characterized in that: The sugar is selected from one of brown sugar, white sugar or honey; the water is selected from one of distilled water, purified water or other clean water sources that meet drinking water standards.

3. The method for preparing agricultural enzymes using wild plant Humulus japonicus according to claim 1, characterized in that: The wild plant is Humulus aviculare, and the Humulus aviculare is fresh stems and leaves from the creeping stage to the blooming stage.

4. The agricultural enzyme prepared by the preparation method according to any one of claims 1 to 3, characterized in that: The agricultural enzyme is brown-yellow to brown-yellow in color, has no obvious odor, has a pH value of 3.0-5.0, an electrical conductivity of 6.0-10.0 mS / cm, an organic matter content of ≥5 g / L, an organic acid content of ≥10 g / L, an amino acid content of ≥0.5 g / L, and contains a variety of plant hormones or hormone-like substances.

5. The agricultural enzyme according to claim 4, characterized in that: The plant hormone or quasi-hormone includes at least one of indoleacetic acid (IAA), jasmonic acid (JA) and salicylic acid (SA).

6. The agricultural enzyme according to claim 4, characterized in that: The organic acid includes at least one of acetic acid, butyric acid and lactic acid.

7. The agricultural enzyme according to claim 4, characterized in that: The amino acid includes at least one of alanine, tyrosine and methionine.

8. The use of the agricultural enzyme according to any one of claims 1 to 7, characterized in that: The agricultural enzyme is diluted 100 to 500 times with water and then applied to crops through irrigation.

9. The use of the agricultural enzyme according to claim 8, characterized in that: Use 400 times diluted solution for vegetable seedlings, and 100 times diluted solution for solanaceous crops. The irrigation frequency is once every 3 to 7 days.

10. The use of the agricultural enzyme according to claim 8, characterized in that: When growing leafy vegetables, use a 500-fold diluted solution and irrigate once every 3 to 7 days.

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