Adjusting device, dual-camera position adjusting device and method and biosynthesis system

Abstract

The invention discloses an adjusting device, a dual-camera position adjusting device and method and a biosynthesis system. The adjusting device comprises a supporting plate, the side portion of the supporting plate is provided with a Z-direction displacement table, the outer side of the Z-direction displacement table has the moving freedom degree parallel to the Z direction, and the Z-direction displacement table is provided with a rotary table; the outer side of the rotary table has a degree of freedom of rotating around an X axis and is provided with an adapter plate; the adapter plate is fixedly connected with the rotary table through a plurality of supporting rods, and the supporting rods have the movement freedom degree in the Y direction. A bearing plate is arranged between the adapter plate and the rotary table, a camera mounting part is arranged on the bearing plate, the bearing plate is connected with the adapter plate through a plurality of joint bearing units, and the plurality of joint bearing units have degrees of freedom for driving the bearing plate to rotate around a Z axis, rotate around a Y axis and move in an X direction. According to the invention, six-degree-of-freedom high-precision and high-stability position adjustment of the camera can be realized, the printing deviation is effectively reduced, the synthesis efficiency is improved, the method is suitable for precise position adjustment and control of a visual system in DNA biosynthesis, and the printing requirement is met.

Description

Technical Field

[0001] The present disclosure relates to the technical field of DNA biosynthesis, and in particular to an adjustment device, a dual-camera position adjustment device and method, and a biosynthesis system. Background Art

[0002] In the technology of synthesizing DNA base chains on the chip surface, for high-throughput synthesis chips, the number of reaction sites is usually over one million, and the reaction sites are circles with a diameter of tens of microns. The margins of the reaction sites are usually also tens of microns. Different designs will be made according to the flux size, site size and spacing. During the synthesis process, the coupling reagent is sprayed into the reaction site by inkjet printing. To achieve precise position printing, it is generally necessary to calculate the relative position of the printing system and the chip through a visual system, and then calibrate the printing position.

[0003] For high-throughput synthesis chips, the size is generally large, and the visual system disclosed in the prior art cannot meet the use requirements in the field of DNA biosynthesis. Summary of the invention

[0004] In view of this, the embodiments of the present disclosure provide an adjustment device, a dual-camera position adjustment device and method, and a biosynthesis system, which are suitable for precise position control of the visual system in the field of DNA biosynthesis to meet printing requirements.

[0005] In a first aspect, an embodiment of the present disclosure provides a regulating device, comprising:

[0006] A support plate, a Z-axis displacement stage is installed on its side, a turntable is installed on the side of the Z-axis displacement stage away from the support plate, and the side of the Z-axis displacement stage away from the support plate has a degree of freedom of movement parallel to the Z-axis direction;

[0007] A transfer plate is installed on the side of the turntable away from the Z-axis translation stage, and the side of the turntable away from the Z-axis translation stage has a degree of freedom to rotate around the X-axis;

[0008] The adapter plate is fixedly connected to the side of the turntable through a plurality of support rods, and the connection between the plurality of support rods and the adapter plate has a degree of freedom of movement parallel to the Y-axis direction;

[0009] A accommodating space is formed between the adapter plate and the turntable; the accommodating space is provided with a carrying plate, the carrying plate has a camera mounting portion, and the carrying plate is connected to the adapter plate through a plurality of joint bearing units, and the plurality of joint bearing units have the freedom to drive the carrying plate to rotate around the Z axis, the freedom to rotate around the Y axis, and the freedom to move in a direction parallel to the X axis.

[0010] Optionally, the plurality of support rods are arranged in parallel and perpendicular to the side of the turntable away from the Z-axis translation stage;

[0011] At least four of the plurality of support rods are provided;

[0012] When four support rods are provided, the plurality of support rods include a first rod, a second rod, a third rod and a fourth rod, and a center line connecting the first rod and the second rod coincides with a diameter of the turntable;

[0013] The third rod and the fourth rod are respectively arranged corresponding to the first rod and the second rod.

[0014] Optionally, the adapter plate is provided with a plurality of long slot holes matching with the plurality of support rods;

[0015] The longitudinal axis of the long slot hole is parallel to the first horizontal direction, and the support rod has the freedom to move along the longitudinal axis of the long slot hole;

[0016] The longitudinal axis of the support rod is parallel to a second horizontal direction, and the second horizontal direction is perpendicular to the first horizontal direction.

[0017] Optionally, three of the plurality of spherical bearing units are provided, and the centers of the three spherical bearing units form a right triangle.

[0018] Optionally, the spherical bearing unit comprises a bearing seat fixedly connected to the bearing plate, a spherical bearing mounted on the bearing seat, a locking cover mounted on one side of the spherical bearing, and an adjusting screw mounted on the other side of the spherical bearing, and the free end of the adjusting screw is connected to the adapter plate through a locking member;

[0019] The locking member includes a first nut and a second nut respectively arranged on both sides of the adapter plate, and the first nut and the second nut are both threadedly connected to the adjusting screw.

[0020] Optionally, the three spherical bearing units are respectively a first unit, a second unit, and a third unit;

[0021] The center line connecting the first unit and the second unit is parallel to the center line connecting the first rod and the second rod;

[0022] The distance between the center of the first unit and the center of the second unit is h, the distance between the center of the first rod and the center of the second rod is H, and H / 2≤h<H.

[0023] Optionally, the camera mounting portion includes a first mounting portion and a second mounting portion, the first mounting portion is provided with a body mounting plate fixed to the camera body; the second mounting portion is provided with a lens limiting plate fixed to the camera lens.

[0024] Optionally, the Z-axis translation stage comprises a first plate fixedly arranged on the side of the support plate, a first guide rail fixedly mounted on the first plate, a second plate, a second guide rail fixedly mounted on the second plate, an adjusting knob and a locking member, wherein the first guide rail and the second guide rail constitute a cross roller guide rail structure, and the longitudinal axis of the cross roller guide rail structure is parallel to the Z direction; the second guide rail has a degree of freedom to move relative to the first guide rail; and the locking member has a degree of freedom to lock the second guide rail;

[0025] The turntable is a manual rotating slide, and the side of the manual rotating slide away from the second plate is parallel to the Z direction and has a rotational freedom around a direction perpendicular to the second plate.

[0026] In a second aspect, the present application discloses a dual-camera position adjustment device, comprising two sets of the adjustment devices, and the support plates in the two sets of the adjustment devices are the same plate;

[0027] The centers of the fields of view of the cameras fixed by the two sets of the adjusting devices coincide with the centers of the two cross marks in the chip to be detected.

[0028] On the third aspect, the present application discloses a biosynthesis system for the biosynthesis of DNA, comprising two sets of visual systems, two sets of light source assemblies and the dual-camera position adjustment device, the two sets of the light source assemblies are respectively matched with the two sets of the visual systems, and the two sets of the light source assemblies are respectively installed on the two supporting plates.

[0029] The adjustment device disclosed in the present application can drive the camera to move up and down along the Z direction through the support rod, the adapter plate, the joint bearing unit, and the bearing plate, that is, the position adjustment of the camera in the Z direction can be performed. The Z-direction displacement is to adjust the distance between the camera lens and the chip surface to adjust the focal length; the outer side of the turntable has the freedom of rotation around the X-axis, and the adapter plate can be driven to rotate around the X-axis through the support rod, that is, the camera can be driven to rotate and adjust around the X-axis; the connection between the support rods and the adapter plate has the freedom of movement parallel to the Y-axis direction, that is, the camera can be driven to move freely and adjust in parallel to the Y-axis direction; the joint bearing units can realize the rotation adjustment of the bearing plate around the Z axis, the rotation adjustment around the Y axis, and the movement adjustment in the direction parallel to the X axis, that is, the rotation adjustment of the camera around the Z axis, the rotation adjustment around the Y axis, and the movement adjustment in the direction parallel to the X axis; through the adjustment device disclosed in the present application, the position adjustment of the six degrees of freedom of the loaded camera can be realized, that is, high-precision visual alignment and high stability can be achieved, the printing deviation can be effectively reduced, and the synthesis efficiency can be improved.

[0030] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the present invention more obvious and easy to understand, the following preferred embodiments are specifically cited and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present disclosure. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0032] Figure 1 This is a three-dimensional schematic diagram of the dual-camera position adjustment device disclosed in the present application during use.

[0033] Figure 2 for Figure 1 Assembly diagram of the Z-axis translation stage and turntable.

[0034] Figure 3 for Figure 1 Installation diagram of the spherical bearing unit.

[0035] Description of reference numerals:

[0036] 10. Camera; 20. Light source assembly; 100. Support plate; 200. Z-axis translation stage; 210. First plate; 220. Second plate; 230. Adjustment knob; 240. Locking member; 300. Turntable; 400. Adapter plate; 500. Support rod; 510. First rod; 520. Second rod; 530. Third rod; 540. Fourth rod; 600. Loading plate; 610. Body mounting plate; 620. Lens stop plate; 700. Joint bearing unit; 710. First unit; 720. Second unit; 730. Third unit. DETAILED DESCRIPTION

[0037] The present disclosure is further described in detail below in conjunction with the accompanying drawings and implementations. It is understood that the specific implementations described herein are only used to explain the relevant content, rather than to limit the present disclosure. It should also be noted that, for ease of description, only the parts related to the present disclosure are shown in the accompanying drawings.

[0038] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments in the present disclosure can be combined with each other. The technical solution of the present disclosure will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0039] Unless otherwise specified, the exemplary embodiments / embodiments shown will be understood as providing exemplary features of various details of some ways in which the technical concept of the present disclosure can be implemented in practice. Therefore, unless otherwise specified, the features of the various embodiments / embodiments can be combined, separated, interchanged and / or rearranged without departing from the technical concept of the present disclosure.

[0040] The use of cross-hatching and / or shading in the accompanying drawings is generally used to make the boundaries between adjacent components clear. As such, unless otherwise specified, the presence or absence of cross-hatching or shading does not convey or indicate any preference or requirement for the specific materials, material properties, dimensions, proportions, commonalities between the components shown, and / or any other characteristics, attributes, properties, etc. of the components. In addition, in the accompanying drawings, the sizes and relative sizes of the components may be exaggerated for clarity and / or descriptive purposes. When the exemplary embodiments can be implemented differently, the specific process sequence can be performed in a different order than described. For example, two successively described processes can be performed substantially simultaneously or in an order opposite to the described order. In addition, the same figure numbers represent the same components.

[0041] When a component is referred to as being "on" or "over," "connected to," or "coupled to" another component, the component may be directly on, directly connected to, or directly coupled to the other component, or intervening components may be present. However, when a component is referred to as being "directly on," "directly connected to," or "directly coupled to" another component, there are no intervening components. For this purpose, the term "connected" may refer to a physical connection, an electrical connection, etc., with or without intervening components.

[0042] For descriptive purposes, the present disclosure may use spatially relative terms such as "under," "beneath," "under," "down," "over," "upper," "above," "higher," and "side (e.g., as in "sidewall")," to describe the relationship of one component to another (other) component as shown in the accompanying drawings. The spatially relative terms are intended to encompass different orientations of the device in use, operation, and / or manufacture in addition to the orientation depicted in the accompanying drawings. For example, if the device in the accompanying drawings is turned over, components described as "under" or "beneath" other components or features would subsequently be positioned "over" the other components or features. Thus, the exemplary term "under" can encompass both the "above" and "below" orientations. Furthermore, the device may be otherwise oriented (e.g., rotated 90 degrees or at other orientations), and the spatially relative descriptors used herein should be interpreted accordingly.

[0043] The terms used here are for the purpose of describing specific embodiments, and are not intended to be restrictive. As used here, unless the context clearly indicates otherwise, the singular forms "one (kind, person)" and "said (the)" are also intended to include plural forms. In addition, when the terms "comprise" and / or "include" and their variations are used in this specification, it is explained that there are stated features, integral bodies, steps, operations, parts, assemblies and / or their groups, but it is not excluded that there are or add one or more other features, integral bodies, steps, operations, parts, assemblies and / or their groups. It should also be noted that, as used here, the terms "substantially", "approximately" and other similar terms are used as approximate terms and not as degree terms, so that they are used to explain the inherent deviations of the measured values, calculated values ​​and / or the values ​​provided that will be recognized by those of ordinary skill in the art.

[0044] Reference Figure 1 The present application discloses a DNA biosynthesis system, including two sets of visual systems, two sets of light source assemblies 20 and dual-camera position adjustment devices; the two sets of light source assemblies 20 are respectively matched with the two sets of visual systems; wherein the dual-camera position adjustment device includes two sets of adjustment devices, and the support plates 100 in the two sets of adjustment devices are the same plate; the field of view centers of the cameras 10 fixed by the two sets of adjustment devices coincide with the centers of the two cross marks in the chip to be detected.

[0045] In this embodiment, the specifications of the chip to be detected are: external dimensions 200x200mm, and the center distance of the cross mark is 170mm. Through the adjustment device disclosed in the application, the center of the field of view of the two cameras 10 can be basically completely overlapped with the center of the cross mark.

[0046] Reference Figure 1 and Figure 2 The adjusting device includes a support plate 100, a Z-axis translation stage 200, a turntable 300, a support rod 500, an adapter plate 400, a bearing plate 600 for mounting the camera 10, and a joint bearing unit 700. The Z-axis translation stage 200 is installed on the side of the support plate 100, and the turntable 300 is installed on the side of the Z-axis translation stage 200 away from the support plate 100, that is, one side of the Z-axis translation stage 200 is fixedly connected to the support plate 100, and the other side (that is, the outer side) has the degree of freedom of lifting and lowering along the Z direction (that is, it has the degree of freedom of movement parallel to the Z-axis direction), which can drive the lifting and lowering of the turntable 300 fixed on the outer side.

[0047] An adapter plate 400 is installed on the side of the turntable 300 away from the Z-axis translation stage 200. The adapter plate 400 is fixedly connected to the side of the turntable 300 through a plurality of support rods 500, and a storage space is formed between the adapter plate 400 and the turntable 300. The outer side surface of the turntable 300 has the freedom to rotate around the X-axis, and the support rods 500 can drive the adapter plate 400 to rotate around the X-axis.

[0048] The Z-axis translation stage 200 includes a first plate 210 fixed to the side of the support plate 100, a first guide rail fixedly mounted on the first plate 210, a second plate 220 (i.e., a slidable plate), a second guide rail fixedly mounted on the second plate 220, an adjusting knob 230, and a locking member 240. The first guide rail and the second guide rail constitute a cross roller guide rail structure, and the longitudinal axis of the cross roller guide rail structure is parallel to the Z direction; the second guide rail has the freedom to move relative to the first guide rail; and the locking member 240 has the freedom to lock the second guide rail. The movement of the second plate 220 can be achieved by controlling the adjusting knob 230 (i.e., a fine-tuning knob), and the locking is performed by the locking member 240 (i.e., a locking screw). The adjusting knob 230 and the second plate 220 are separate structures, and force can only be applied in one direction. When rotating, the second plate 220 is reset by a spring.

[0049] Furthermore, the flatness of the second plate 220 is preferably about 5 micrometers.

[0050] The turntable 300 is a L / R manual rotating slide, which can realize 360-degree coarse adjustment and ±5-degree fine adjustment. Specifically, the turntable has a precision copper sleeve bearing inside, and the turntable can rotate 360 ​​degrees after the coarse adjustment is loosened. After the coarse adjustment, the fine adjustment knob can be used for fine adjustment (±5°), and after the fine adjustment, it is fixed by opposite locking.

[0051] The side of the manual rotating slide away from the second plate 220 is parallel to the Z direction and has rotational freedom. Specifically, the side of the manual rotating slide away from the second plate 220 has rotational freedom around a direction perpendicular to the second plate 220 (ie around the X axis).

[0052] The plurality of supporting rods 500 are all arranged in parallel and perpendicular to the side of the turntable 300 away from the Z-axis translation stage 200 , so as to ensure that the plane of the adapter plate 400 is parallel to the outer side of the turntable 300 .

[0053] Furthermore, at least four supporting rods 500 are provided to ensure the installation stability of the adapter plate 400 and the outer side surface of the turntable 300 .

[0054] When four support rods 500 are provided, the plurality of support rods 500 include a first rod 510, a second rod 520, a third rod 530 and a fourth rod 540, and a center line connecting the first rod 510 and the second rod 520 coincides with a diameter of the turntable 300; the third rod 530 and the fourth rod 540 are provided corresponding to the first rod 510 and the second rod 520 respectively.

[0055] In this embodiment, the adapter plate 400 is provided with a plurality of long slots matching the plurality of support rods 500; the longitudinal axis of the long slots is parallel to the first horizontal direction (eg, the Y axis), and the support rods 500 have the freedom to move along the longitudinal axis of the long slots;

[0056] The longitudinal axis of the support rod 500 is parallel to the second horizontal direction (eg, the X-axis), and the second horizontal direction is perpendicular to the first horizontal direction.

[0057] Since the support rod 500 is fixedly connected to the outer side of the turntable 300, the adapter plate 400 can freely move in the Y direction relative to the turntable 300 through a plurality of long slots, that is, it has a degree of freedom of movement parallel to the Y-axis direction.

[0058] A carrying plate 600 is arranged in the accommodating space (that is, the carrying plate 600 is arranged between the adapter plate 400 and the turntable 300), and a camera mounting portion is provided on the carrying plate 600. In this embodiment, the camera mounting portion includes a first mounting portion and a second mounting portion. The first mounting portion is provided with a body mounting plate 610 fixed to the body of the camera 10; the second mounting portion is provided with a lens limiting plate 620 fixed to the lens of the camera 10, so as to realize the position constraint of the lens so as to stably install the camera 10, that is, the camera 10 and the carrying plate 600 constitute a whole and move synchronously.

[0059] A plurality of joint bearing units 700 are mounted on the bearing plate 600 ; a plurality of adjustment holes are opened on the adapter plate 400 , and the free ends of the plurality of joint bearing units 700 are respectively connected to the plurality of adjustment holes, and each joint bearing unit 700 has the freedom of movement relative to the adapter plate 400 .

[0060] Specifically, the joint bearing unit 700 includes a bearing seat fixedly connected to the bearing plate 600, a joint bearing installed on the bearing seat, a locking cover installed on one side of the joint bearing, and an adjusting screw installed on the other side of the joint bearing. The free end of the adjusting screw is connected to the adapter plate 400 through a locking member 240; the locking member 240 includes a first nut and a second nut respectively arranged on both sides of the adapter plate 400, and the first nut and the second nut are both threadedly connected to the adjusting screw.

[0061] In this embodiment, three spherical bearing units 700 are preferably provided, and the centers of the three spherical bearing units 700 form a right triangle.

[0062] Also refer to Figure 3 The three joint bearing units 700 are respectively the first unit 710, the second unit 720, and the third unit 730; the center line of the first unit 710 and the second unit 720 is parallel to the center line of the first rod 510 and the second rod 520; the distance between the center of the first unit 710 and the center of the second unit 720 is h, and the distance between the center of the first rod 510 and the center of the second rod 520 is H, H / 2≤h<H, ensuring the load-bearing stability of the load-bearing plate 600 through the adapter plate 400.

[0063] Based on the principle that three points determine a plane, by separately adjusting the length of the adjusting screw in the first unit 710, the rotation adjustment of the supporting plate 600 around the Z axis can be achieved; by separately adjusting the length of the adjusting screw in the third unit 730, the rotation adjustment of the supporting plate 600 around the Y axis can be achieved; by simultaneously adjusting the lengths of the adjusting screws in the first unit 710, the second unit 720, and the third unit 730, the movement adjustment of the supporting plate 600 along a direction parallel to the X axis can be achieved.

[0064] The adjustment device disclosed in the present application can lift and lower the outer side surface of the Z-axis displacement stage 200 through the support rod 500, the adapter plate 400, the joint bearing unit 700, and the bearing plate 600, so that the camera 10 can be lifted and lowered along the Z-axis, that is, the position of the camera 10 in the Z-axis can be adjusted. The Z-axis displacement is to adjust the distance between the camera 10 lens and the chip surface to adjust the focal length; the outer side surface of the turntable 300 has the degree of freedom to rotate around the X-axis, and the adapter plate 400 can be driven to rotate around the X-axis through the support rod 500, that is, the camera 10 can be driven to rotate and adjust around the X-axis; a plurality of support rods 500 and 600 can be used to drive the adapter plate 400 to rotate around the X-axis. The connection between the rod 500 and the adapter plate 400 has a degree of freedom of movement parallel to the Y-axis direction, that is, it can drive the camera 10 to perform free movement adjustment parallel to the Y-axis direction; a number of joint bearing units 700 can realize the rotation adjustment of the carrier plate 600 around the Z-axis, the rotation adjustment around the Y-axis, and the movement adjustment along the direction parallel to the X-axis, that is, the rotation adjustment of the camera 10 around the Z-axis, the rotation adjustment around the Y-axis, and the movement adjustment along the direction parallel to the X-axis; through the adjustment device disclosed in the present application, the position adjustment of the six degrees of freedom of the loaded camera 10 can be realized.

[0065] In this embodiment, each set of visual system is installed to match the camera mounting part; the two sets of visual systems and the two sets of light source assemblies 20 are fixedly installed on the supporting plate 600, the support plates 100 in the two sets of adjustment devices are the same plate, the two sets of adjustment devices are installed at the same height, and the two sets of light source assemblies 20 are respectively installed on the two supporting plates 600.

[0066] Furthermore, the center position between the two sets of adjustment devices is set to coincide with the center of the support plate 100 to ensure installation stability.

[0067] Since the relative position of the camera 10 to the printing system and the chip is imprecise and cannot be determined when it is installed, the chip position can be fixed first, and the positions of the two cameras 10 can be adjusted based on the chip to achieve precise adjustment of the relative position of the visual system and the chip, thereby ensuring higher alignment accuracy.

[0068] Based on the above application scenarios and requirements, in the dual-camera position adjustment device, each camera can independently adjust the position with 6 degrees of freedom, including displacement in the X direction, displacement in the Y direction, displacement in the Z direction, rotation around the X axis, rotation around the Y axis, and rotation around the Z axis; the size and position accuracy of the two Marks on the chip are at the micron level, which is very accurate, so the relative position of the two cameras 10 can be judged and adjusted by the position of the Mark in the field of view of each camera 10. By precisely adjusting the spatial position of the two cameras 10 and locking them, high-precision visual alignment and high stability can be achieved, effectively reducing printing deviations and improving synthesis efficiency.

[0069] The adjustment device disclosed in the present application has several significant advantages in the field of DNA biosynthesis of high-throughput synthesis chips, including: providing multiple degrees of freedom adjustment capabilities through the combination of a Z-axis translation stage, a turntable and a support rod, ensuring that the camera can be precisely adjusted in the X, Y, and Z axis directions and in the rotation directions around the X, Y, and Z axes, which enables the camera to be accurately aligned with the cross mark on the chip, thereby improving the accuracy of printing; the relative position of the printing system and the chip is calculated through a visual system, and then the printing position is calibrated. The multi-degree-of-freedom adjustment can ensure that the camera can capture the image of the reaction site from the best viewing angle, thereby improving the accuracy of the calibration.

[0070] In this application, the carrier plate is connected to the adapter plate through a joint bearing unit, and can rotate around the Z axis and Y axis, and move along the X axis, so that the camera can be flexibly adjusted according to the design of different chips and the layout of reaction sites, and adapt to reaction sites of different sizes and spacings; the accommodation space formed between the adapter plate and the turntable allows the carrier plate and the camera to be placed in a relatively fixed environment, reducing the impact of the external environment and improving stability and reliability; the multi-degree-of-freedom adjustment capability allows the camera to quickly align with the cross mark on the chip, reducing calibration time and steps and improving work efficiency; high-precision positioning and calibration reduce synthesis failures or inefficient synthesis due to position errors and improve the efficiency of the overall synthesis process.

[0071] This device is particularly suitable for high-throughput synthesis chips. The required precise position can be achieved by simply adjusting the center of the camera's field of view to align with the cross mark on the chip, because the cross mark and the reaction site on the chip have absolute physical positions when the chip is processed. After the camera is aligned with the cross mark to determine the position of the cross mark, the position of the reaction site can be obtained through the algorithm. Therefore, it can ensure that each reaction site can be accurately printed at the same time to meet the needs of large-scale production. The design of the device allows adjustment according to different chip sizes and site layouts, has good scalability, and can adapt to production needs of different scales and types; multi-degree-of-freedom adjustment and high-precision positioning enable the device to remain stable during long-term use, reducing maintenance costs caused by error accumulation, and the design of the joint bearing unit and the support rod allows the device to be easily disassembled and maintained, reducing the cost of repair and replacement of parts.

[0072] In summary, the regulating device disclosed in the present application significantly improves the accuracy, efficiency and adaptability of the DNA biosynthesis process through multi-degree-of-freedom adjustment and high-precision positioning, is particularly suitable for the production of high-throughput synthesis chips, and has important practical application value.

[0073] Another aspect of the present application discloses a dual-camera position adjustment method, based on the biosynthesis system, comprising:

[0074] S100, respectively obtaining the positions of the centers of two cross marks in the chip to be inspected through two sets of visual systems;

[0075] S200, determining, according to the positions of the centers of the two cross marks, the distance between the centers of the two cross marks, a first deviation between the visual center of the first visual system and the center of the first cross mark, and a second deviation between the visual center of the second visual system and the center of the second cross mark;

[0076] The first deviation includes a first X-axis offset value, a first Y-axis offset value, and a first angle offset value; the second deviation includes a second X-axis offset value, a second Y-axis offset value, and a second angle offset value.

[0077] Among them, the first X-axis offset value represents the offset of the visual center of the first visual system relative to the center of the first cross mark in the X-axis direction; the first Y-axis offset value represents the offset of the visual center of the first visual system relative to the center of the first cross mark in the Y-axis direction; the first angle offset value represents the offset of the visual center of the first visual system relative to the center of the first cross mark in the angle direction (that is, whether the camera needs to be rotated to align with the center of the cross mark).

[0078] The second X-axis offset value represents the offset of the visual center of the second visual system relative to the center of the second cross mark in the X-axis direction; the second Y-axis offset value represents the offset of the visual center of the second visual system relative to the center of the second cross mark in the Y-axis direction; the second angular offset value represents the offset of the visual center of the second visual system relative to the center of the second cross mark in the angular direction.

[0079] S300, based on the first deviation amount and the second deviation amount, respectively adjust the positions of the corresponding visual systems through the dual-camera position adjustment devices until the centers of the fields of view of the cameras fixed by the two adjustment devices respectively coincide with the centers of the two cross marks in the chip to be inspected.

[0080] Based on the first deviation and the second deviation obtained by the above calculation, the system adjusts the positions of the two cameras respectively through the dual-camera position adjustment device.

[0081] Adjustment of the first camera: according to the first X-axis offset value, the adjusting device moves the first camera in the X-axis direction so that the visual center is aligned with the X coordinate of the center of the first cross mark; according to the first Y-axis offset value, the adjusting device moves the first camera in the Y-axis direction so that the visual center is aligned with the Y coordinate of the center of the first cross mark; according to the first angle offset value, the adjusting device rotates the first camera so that the angle of the visual center is aligned with the angle of the center of the first cross mark.

[0082] Adjustment of the second camera: According to the second X-axis offset value, the adjusting device moves the second camera in the X-axis direction so that its visual center is aligned with the X coordinate of the center of the second cross mark; according to the second Y-axis offset value, the adjusting device moves the second camera in the Y-axis direction so that its visual center is aligned with the Y coordinate of the center of the second cross mark; according to the second angle offset value, the adjusting device rotates the second camera so that the angle of its visual center is aligned with the angle of the center of the second cross mark.

[0083] The process is iterative, and the system will continuously detect whether the visual centers of the two cameras completely overlap with the centers of the cross marks. If there is still a deviation, the system will continue to adjust until the centers of the field of view of the two camera systems are completely aligned with the centers of the two cross marks.

[0084] When the visual centers of the two cameras completely coincide with the centers of the two cross marks, the adjustment process is completed and the system enters the normal working state, ready for subsequent detection or measurement tasks.

[0085] Through the above steps, the dual-camera position adjustment method can accurately adjust the positions of the two camera systems to ensure that they can accurately align with the centers of the two cross marks in the chip to be inspected, thereby improving the accuracy and stability of the inspection.

[0086] In the description of this specification, the description with reference to the terms "one embodiment / method", "some embodiments / methods", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment / method or example are included in at least one embodiment / method or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment / method or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments / methods or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments / methods or examples described in this specification and the features of the different embodiments / methods or examples, unless they are contradictory.

[0087] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0088] Those skilled in the art should understand that the above embodiments are only for the purpose of clearly illustrating the present disclosure, and are not intended to limit the scope of the present disclosure. For those skilled in the art, other changes or modifications may be made based on the above disclosure, and these changes or modifications are still within the scope of the present disclosure.

Claims

1. A regulating device, characterized in that: include: A support plate, a Z-axis displacement stage is installed on its side, a turntable is installed on the side of the Z-axis displacement stage away from the support plate, and the side of the Z-axis displacement stage away from the support plate has a degree of freedom of movement parallel to the Z-axis direction; A transfer plate is installed on the side of the turntable away from the Z-axis translation stage, and the side of the turntable away from the Z-axis translation stage has a degree of freedom to rotate around the X-axis; The adapter plate is fixedly connected to the side of the turntable through a plurality of support rods, and the connection between the plurality of support rods and the adapter plate has a degree of freedom of movement parallel to the Y-axis direction; A accommodating space is formed between the adapter plate and the turntable; the accommodating space is provided with a carrying plate, the carrying plate has a camera mounting portion, and the carrying plate is connected to the adapter plate through a plurality of joint bearing units, and the plurality of joint bearing units have the freedom to drive the carrying plate to rotate around the Z axis, the freedom to rotate around the Y axis, and the freedom to move in a direction parallel to the X axis.

2. The adjusting device according to claim 1, characterized in that: The plurality of support rods are arranged in parallel and perpendicular to the side of the turntable away from the Z-axis translation stage; At least four of the plurality of support rods are provided; When four support rods are provided, the plurality of support rods include a first rod, a second rod, a third rod and a fourth rod, and a center line connecting the first rod and the second rod coincides with a diameter of the turntable; The third rod and the fourth rod are respectively arranged corresponding to the first rod and the second rod.

3. The adjusting device according to claim 2, characterized in that: The adapter plate is provided with a plurality of long slots matching the plurality of support rods; The longitudinal axis of the long slot hole is parallel to the first horizontal direction, and the support rod has the freedom to move along the longitudinal axis of the long slot hole; The longitudinal axis of the support rod is parallel to a second horizontal direction, and the second horizontal direction is perpendicular to the first horizontal direction.

4. The adjusting device according to claim 2, characterized in that: There are three of the plurality of spherical bearing units, and the centers of the three spherical bearing units form a right triangle.

5. The adjusting device according to claim 4, characterized in that: The spherical bearing unit comprises a bearing seat fixedly connected to the bearing plate, a spherical bearing mounted on the bearing seat, a locking cover mounted on one side of the spherical bearing, and an adjusting screw mounted on the other side of the spherical bearing, wherein the free end of the adjusting screw is connected to the adapter plate via a locking member; The locking member includes a first nut and a second nut respectively arranged on both sides of the adapter plate, and the first nut and the second nut are both threadedly connected to the adjusting screw.

6. The adjusting device according to claim 4, characterized in that: The three spherical bearing units are respectively a first unit, a second unit and a third unit; The center line connecting the first unit and the second unit is parallel to the center line connecting the first rod and the second rod; The distance between the center of the first unit and the center of the second unit is h, the distance between the center of the first rod and the center of the second rod is H, and H / 2≤h<H.

7. The adjusting device according to claim 1, characterized in that: The camera mounting portion includes a first mounting portion and a second mounting portion, wherein the first mounting portion is provided with a body mounting plate fixed to the camera body; and the second mounting portion is provided with a lens limiting plate fixed to the camera lens.

8. The adjustment device according to claim 1, characterized in that: The Z-axis translation stage comprises a first plate fixedly arranged on the side of the support plate, a first guide rail fixedly mounted on the first plate, a second plate, a second guide rail fixedly mounted on the second plate, an adjusting knob and a locking member, wherein the first guide rail and the second guide rail constitute a cross roller guide rail structure, and the longitudinal axis of the cross roller guide rail structure is parallel to the Z direction; the second guide rail has a degree of freedom to move relative to the first guide rail; and the locking member has a degree of freedom to lock the second guide rail; The turntable is a manual rotating slide, and the side of the manual rotating slide away from the second plate is parallel to the Z direction and has a rotational freedom around a direction perpendicular to the second plate.

9. A dual-camera position adjustment device, characterized in that: Comprising two sets of the adjusting devices according to any one of claims 1 to 8, wherein the support plates in the two sets of the adjusting devices are the same plate; The centers of the fields of view of the cameras fixed by the two sets of the adjusting devices coincide with the centers of the two cross marks in the chip to be detected.

10. A biosynthesis system for biosynthesis of DNA, characterized in that: It comprises two sets of visual systems, two sets of light source assemblies and the dual-camera position adjustment device as described in claim 9, the two sets of light source assemblies are respectively matched with the two sets of visual systems, and the two sets of light source assemblies are respectively installed on the two supporting plates.

11. A dual-camera position adjustment method, characterized in that: The biosynthetic system according to claim 10, comprising: S100, respectively obtaining the positions of the centers of two cross marks in the chip to be inspected through two sets of visual systems; S200, determining, according to the positions of the centers of the two cross marks, the distance between the centers of the two cross marks, a first deviation between the visual center of the first visual system and the center of the first cross mark, and a second deviation between the visual center of the second visual system and the center of the second cross mark; The first deviation includes a first X-axis offset value, a first Y-axis offset value, and a first angle offset value; the second deviation includes a second X-axis offset value, a second Y-axis offset value, and a second angle offset value; S300, based on the first deviation amount and the second deviation amount, the positions of the corresponding visual systems are adjusted respectively by the dual-camera position adjustment device until the centers of the fields of view of the cameras fixed by the two sets of the adjustment devices coincide with the centers of the two cross marks in the chip to be inspected.

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