Application of 1-octylene-3-ol in prevention and control of solenopsis invicta

Abstract

The invention discloses an application of 1-octylene-3-alcohol in prevention and control of solenopsis invicta. The 1-octylene-3-alcohol has toxicity to the solenopsis invicta, and behavior analysis shows that the 1-octylene-3-alcohol also affects various social behaviors of the solenopsis invicta, including gathering and nest cleaning (corpse discarding) behaviors, so that the control effect on the solenopsis invicta is achieved. The 1-octylene-3-alcohol can also be used as a repelling odor source to generate repelling effect on the solenopsis invicta, so that the solenopsis invicta is prevented from diffusing or invading to a specific area. 1-octylene-3-alcohol has the characteristics of being volatile, non-toxic, free of residues, insoluble in water and the like, does not cause soil residue pollution, is not influenced by weather, is safer and more effective, and has a wide application prospect in prevention and control of solenopsis invicta.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to application of 1-octen-3-ol in preventing and controlling red imported fire ants. Background Art

[0002] The red fire ant (Solenopsis invicta Buren) is native to the Panama River Basin in South America and is recognized as one of the 100 most dangerous invasive species in the world. It has the characteristics of fast reproduction, strong competitiveness and great destructive power. After invading, it can cause disasters in a short period of time, seriously affecting agricultural production, people's lives and ecological environment in the affected areas. It has now invaded 12 provinces in my country, and the invasion area continues to expand northward and westward. It occurs more frequently in sunny places such as ridges, riverbanks, wastelands, parks, highways, community green belts and adjacent woodlands. The occurrence in farmland and woodlands is less serious than in green areas. The highest altitude reaches 2,800m, showing a natural spread trend from south to north, from low-temperature river valleys to cold areas, and from low-altitude areas to high-altitude areas. New counties and districts are invaded every year.

[0003] Red fire ants pose a major threat to agricultural and forestry production, animal health, and biodiversity. They cause serious damage to the ecosystem of local ants by replacing local ants. For example, the invasion of red fire ants has had a negative impact on the population density and biodiversity of small arthropods (springtails and mites), thereby destroying the ecological function of the soil within the agricultural system. Similarly, the invasion of red fire ants has damaged the seeds of dryland crops including wheat, sorghum, corn, soybeans, and cotton, leading to a decline in agricultural yields. In addition, the presence of red fire ants has been shown to change the physical and chemical properties of agricultural soils, with the carbon-nitrogen ratio, organic matter content, and pH value of anthills being significantly higher than those of nearby soils. Stings by red fire ants may cause allergic reactions (redness, itching, swelling), and in severe cases, may lead to acute chest tightness, difficulty breathing, and even shock, which has a huge impact on agricultural production and tourism and leisure.

[0004] The existing control methods for red fire ants are: (1) Independent anthill treatment method: In areas with severe and moderate damage, by pouring medicine or using powders or granules to directly treat visible anthills, more than 98% of anthills can be effectively controlled. However, this method can only treat visible anthills, and it is easy to miss newly established ant nests that have not produced obvious anthills; and most of the drenching products need to add 5-10 liters of medicine to each ant nest to be effective, which requires a large amount of medicine, high cost, and is easy to cause land drug residue pollution. (2) Bait method: The red fire ant bait used is mostly granular or powdery, such as ant killer bait, red ant net, etc. When using, about 10-30 grams of bait needs to be placed in the ant nest or sprinkled in the ant area. Its action time is relatively slow, and it has the disadvantages of a short service life and being greatly affected by weather factors (no rain is required within 24 hours).

[0005] The existing independent anthill treatment method has problems such as omissions in the prevention and control of new ant nests, high cost of watering and easy to cause residual pollution in the land, and bait method is greatly affected by the weather. Therefore, it is extremely necessary to develop new red fire ant control agents.

[0006] 1-Octene-3-ol (CAS No.: 3391-86-4, molecular formula C 8 H 16 O, structural formula is CH 2 =CHCH(OH)CH 2 CH 2 CH 2 CH 2 CH 3 , English name 1-octen-3-ol), is a colorless to light yellow oily liquid, is a edible flavoring permitted for use in accordance with GB 2760-2014 and GB29976-2013, and can be extracted from a variety of spice crops or synthesized artificially. 1-Octene-3-ol has the characteristics of being volatile, non-toxic, residue-free and insoluble in water. So far, there is no report on the control effect of 1-octen-3-ol on red fire ants, especially red fire ants can be controlled by fumigation, and the dosage of 1-octen-3-ol is extremely small, the effect is remarkable, and it has significant progress. Summary of the invention

[0007] In view of this, one of the objects of the present invention is to provide a use of 1-octen-3-ol in preventing and controlling red imported fire ants.

[0008] Preferably, the controlling of red fire ants includes killing red fire ants and / or reducing the social behavior ability of red fire ants.

[0009] Preferably, the social behavior ability includes the ability to gather and / or the ability to abandon corpses.

[0010] Preferably, 1-octen-3-ol is controlled by fumigation.

[0011] Preferably, the fumigation concentration of 1-octen-3-ol is greater than or equal to 0.0702 μg / cm3.

[0012] Preferably, the fumigation concentration is 0.0702 μg / cm3.

[0013] The 1-octen-3-ol of the present invention is toxic to red fire ants, and can be used to kill red fire ants by fumigation, contact or diet, so as to prevent and control. 1-octen-3-ol can also interfere with the chemical communication signal transmission between red fire ant colonies, making it difficult for them to accurately identify the same species and nest environment information. In terms of aggregation behavior, after being treated with 1-octen-3-ol, the mutual attraction and aggregation coordination between red fire ants are destroyed, and the originally tight and orderly group structure becomes loose, reducing their collective foraging, defense against external enemies, and the efficiency of building and maintaining nests. For nest cleaning (abandoning corpses) behavior, the substance causes red fire ants to perceive and handle dead same species or nest debris abnormally, so that the sanitary conditions in the nest deteriorate, easily cause disease transmission, and then weaken the survival ability and reproductive success rate of the entire ant colony. This destructive effect on the social behavior of red fire ants disintegrates their survival system from within the ant colony. Compared with traditional prevention and control methods that only kill individuals, it has a more comprehensive, in-depth and long-lasting prevention and control effect. It has opened up new directions and strategies for the comprehensive prevention and control of red fire ants and has an application value that cannot be ignored in the field of red fire ant management.

[0014] The second object of the present invention is to provide the use of 1-octen-3-ol in repelling red imported fire ants.

[0015] Preferably, the concentration of 1-octen-3-ol as the repellent odor source is greater than or equal to 0.83 mg / mL.

[0016] Preferably, the concentration of the repellent odor source is 0.83 mg / mL.

[0017] The special smell of 1-octen-3-ol can effectively interfere with the olfactory perception system of red fire ants, causing them to actively avoid the corresponding area after sensing the smell, thereby preventing the red fire ants from spreading or invading specific areas. This repellent effect is highly efficient and lasting. Compared with traditional physical isolation or chemical repellents, 1-octen-3-ol will not cause additional pollution and damage to the environment, and its range of action is wider. It can form an effective protection area in a larger area, effectively reducing the damage caused by red fire ants to crops, landscapes, and human living environments, showing unique advantages and great application potential in the field of ecological prevention and control of red fire ants.

[0018] The third object of the present invention is to provide the use of red fire ants killed by 1-octen-3-ol in preventing and controlling normal red fire ants.

[0019] Preferably, the control of normal red fire ants is that normal red fire ants give up cleaning up the corpses of red fire ants killed by 1-octen-3-ol, causing environmental pollution, thereby controlling them.

[0020] Preferably, the concentration of 1-octen-3-ol in the red fire ants killed by 1-octen-3-ol is greater than or equal to 0.702 μg / cm3.

[0021] Preferably, the concentration of 1-octen-3-ol is 0.702 μg / cm3.

[0022] Normal red fire ants are those that have not been treated with 1-octen-3-ol. Normal red fire ants will give up cleaning up the bodies of red fire ants killed by 1-octen-3-ol. The bodies of red fire ants killed by 1-octen-3-ol will also have a series of negative effects on the ant colony, such as disease transmission, deterioration of the nest environment, reduced ant work efficiency, survival ability, reproduction rate, and attraction of natural enemies and pests, which will eventually lead to a reduction in the number of ant colonies or even extinction, thus achieving a control effect.

[0023] In summary, the present invention uses 1-octen-3-ol to prevent and treat red fire ants, and 1-octen-3-ol is toxic to red fire ants, and behavioral analysis shows that 1-octen-3-ol also affects a variety of social behaviors of red fire ants, including aggregation and nest cleaning (abandoning corpse) behavior, so as to achieve the control effect of red fire ants. 1-octen-3-ol can also be used as a repellent odor source to produce a repellent effect on red fire ants, thereby preventing red fire ants from spreading or invading a specific area. 1-octen-3-ol has the characteristics of being volatile, non-toxic, non-residual and insoluble in water, does not cause residual pollution of the land, and is safer and more effective. Thus, 1-octen-3-ol avoids the existing independent anthill treatment method to prevent and treat the new ant nest omission, the high cost of the irrigation method and the easy cause of residual pollution of the land, the bait method is greatly affected by the weather, and the problems, especially can be controlled by fumigation method Red fire ants, and 1-octen-3-ol dosage is extremely small, the effect is remarkable, there is a significant improvement, and there is a broad application prospect in the prevention and treatment of red fire ants. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is the survival curve of red imported fire ants exposed to different concentrations of 1-octen-3-ol;

[0025] Figure 2 is the half-lethal time of fumigation with different concentrations of 1-octen-3-ol;

[0026] Figure 3 The effect of different concentrations of 1-octen-3-ol on the approach and avoidance behavior of red imported fire ants;

[0027] Figure 4 The differences in aggregation behavior of red imported fire ants exposed to 1-octen-3-ol;

[0028] Figure 5 The differences in the discarding behaviors of healthy red fire ant workers towards the carcasses of two types of red fire ants killed by freezing and 1-octen-3-ol respectively;

[0029] Figure 6The differences in the corpse abandonment behaviors of surviving red fire ants exposed to 1-octen-3-ol and methanol (control group) respectively towards freeze-killed corpses. DETAILED DESCRIPTION

[0030] The present invention will be described in detail below in conjunction with the examples, which are only illustrative and are not limited to the scope of application of the present invention. The present invention is not limited to the following embodiments or examples, and any modifications and variations that do not violate the spirit of the present invention should be included in the scope of the present invention. Unless otherwise specified, the experimental reagents used in the following examples can be purchased through commercial channels. The 1-octen-3-ol (abbreviated as: octenol, purity ≥98.0%) used in the following examples was purchased from Shanghai McLean Biochemical Technology Co., Ltd.; the red fire ants used were collected from Moon Lake Park, Xingyi City, Qianxinan Autonomous Prefecture, Guizhou Province.

[0031] Example 1: Toxicity test of 1-octen-3-ol to red imported fire ants (fumigation method)

[0032] Fifteen red fire ant workers were placed in a 2-ounce plastic cup with talcum powder on the inner wall. Two centrifuge tube caps were placed in each plastic cup; one cap contained 50 μL of 10% sucrose solution, while the other cap contained 0.5 μL of different concentrations of octenol methanol solution (1-1000 times dilution of octenol in methanol), and the final concentrations of octenol reached 7.02, 0.0702, 0.0702 and 0.00702 μg / cm3 respectively (Note: 7.02 μg / cm3 means 7.02 μg of octenol per cubic centimeter of space volume, and the rest are similar). The cup was then tightly sealed. The control group cap was treated with (0.5 μL) methanol. The fumigation activity was evaluated by counting the number of surviving worker ants every 12 hours for 5 days. The cumulative survival rate was calculated according to the following formula: Survival rate (%) = number of surviving test ants / total number of test ants × 100. Each treatment contained three technical replicates, and the entire experiment was repeated using three independent batches of worker ants.

[0033] Fumigation efficacy: The survival of red fire ant workers was examined in a series of octenol fumigation experiments over a period of 5 days ( Figure 1 The calculated time to reach 50% mortality (LT50) for ants treated with the highest concentration of octenol tested (7.02 μg / cm3) was 0.29 days ( Figure 2). When the octenol concentration was reduced by 10 times (0.702 μg / cm3), the LT50 value increased to 3 days, and the cumulative mortality rate after 5 days of treatment was 91%. When the octenol concentration was reduced (0.0702 μg / cm3 and 0.00702 μg / cm3), the LT50 increased to 4.4 days and 6.0 days, respectively, and the cumulative mortality rate after 5 days of treatment was reduced to 87% and 37%, respectively. Based on these data, the half lethal concentration (LC50, half lethal concentration) of octenol was determined to be 0.0702 μg / cm3 using SPSS software probit.

[0034] Example 2: Test on the avoidance behavior of 1-octen-3-ol on red imported fire ants

[0035] The behavioral choice experiment of red imported fire ants on octenol was conducted using a Y-type olfactometer. The indoor lighting was provided by an LED light directly above the Y-tube apparatus. The experiment was conducted at a temperature of 25±2℃ and a relative humidity of 60±5%. The gas flow rate in the Y-tube was 60mL / s. Six concentrations of octenol methanol solutions were prepared by diluting the pure solution of 830mg / mL octenol in methanol in a 10-fold gradient to 100,000 times. For each test condition, 10μL of sample (octenol methanol solution or control methanol) was added to a filter paper strip (3cm×3cm), which was then inserted into one arm of the Y-tube as an odor source. A filter paper strip of the same size containing (10μL) methanol was placed in the other arm of the Y-tube as a control. A test ant was placed at the base end of the Y-type olfactometer, and if the ant crossed two-thirds of the length of the Y-branch arm within 5 minutes, it was recorded as "choice"; otherwise, it was recorded as "no choice" (not included in the data). To ensure no directional bias, the Y-shaped tube arm connected to the odor source was switched to the other arm tube after testing 5 ants. A total of 15 ants were tested in each treatment. Each treatment contained three technical replicates (15 ants per replicate), and the entire experiment was repeated using three independent batches of worker ants.

[0036] Avoidance behavior effect: The Y-tube olfactory test was performed as described in the Materials and Methods section to examine the response of red fire ants to octenol. Red fire ant workers showed significant (P<0.01) avoidance behavior at concentrations of 830 mg / mL, 83 mg / mL, 8.3 mg / mL, and 0.83 mg / mL octenol, with avoidance levels reaching 94.2%, 91.2%, 76.7%, and 72.2%, respectively. Figure 3 No avoidance behavior was observed at the lower tested concentrations of 0.083 mg / mL and 0.0083 mg / mL.

[0037] Example 3: Aggregation behavior test of 1-octen-3-ol on red imported fire ants

[0038] To measure aggregation, the number of responses of individual fire ants to 0.0702 μg / cm3 octenol was examined. Aggregation was considered positive when the ants were placed at the bottom of the cup for one hour or more. Aggregation was defined as the formation of a colony consisting of three or more ants. Aggregation levels were calculated using the following formula: Aggregation (%) = Number of ants aggregated / Total number of ants × 100. Each treatment contained 20 ants and was repeated three times. The entire experiment was repeated using three independent batches of worker ants.

[0039] Aggregation behavior: 97% of red fire ant workers showed aggregation behavior under methanol control exposure conditions, while under 0.0702 μg / cm3 octenol treatment, the aggregation behavior was reduced to 13.3% (P<0.001, Figure 4 ). This indicates that 1-octen-3-ol reduces the aggregation behavior of red imported fire ants.

[0040] Example 4: 1-octen-3-ol test on nest cleaning (corpse abandonment) behavior of red imported fire ants

[0041] Two types of experiments were conducted to investigate the effects of octenol exposure on the corpse-carrying behavior (corpse removal) of red imported fire ants: (a) 15 untreated live workers were placed in a box containing five frozen ant corpses and five ant corpses killed by octenol (0.702 μg / cm3), with four corpses arranged in a square and the remaining one placed in the center of the square. Three days later, the removal of any dead corpses to the “corpse dump” by live workers was recorded. (b) 30 healthy workers were first exposed to octenol (0.0702 μg / cm3) or methanol, and 15 surviving workers were selected after three days of exposure and placed in a box containing five frozen ant corpses. Three days later, the removal of any dead corpses to the “corpse dump” by live workers was recorded. Each experiment contained three technical replicates (15 ants in each replicate). The entire experiment was repeated using three independent batches of worker ants.

[0042] Effect of nest cleaning (discarding carcasses and moving carcasses to the carcass dump): 100% of the ants killed by freezing were moved by healthy workers treated with methanol, while only 33% of the ants killed by octenol were moved (P < 0.01, Figure 5 When the surviving workers (survivors) previously exposed to octenol were used for corpse abandonment behavior analysis, the corpse carrying behavior of these octenol-exposed surviving workers to the ants killed by freezing was reduced to 27%, while the methanol-exposed control workers to the ants killed by freezing maintained 100% corpse abandonment response (P<0.01, Figure 6 ). This indicates that octenol reduces the ability of red fire ants to abandon corpses, and healthy red fire ants will reduce the cleaning of ants killed by octenol.

[0043] In summary, 1-octen-3-ol is toxic to red fire ants and affects their behavioral abilities, and can be used to prevent and control red fire ants.

[0044] The conventional techniques and schemes not described in detail in the above embodiments are well known in the art, so they will not be described in detail here. The above embodiments and / or experimental examples describe the preferred embodiments of the present invention in detail, but the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, the technical scheme of the present invention can be subjected to a variety of simple modifications, and these simple modifications all belong to the protection scope of the present invention.

Claims

1. Application of 1-octen-3-ol in preventing and controlling red fire ants.

2. The use according to claim 1, characterized in that The controlling of red fire ants includes killing the red fire ants and / or reducing the social behavior ability of the red fire ants.

3. The use according to claim 2, characterized in that The social behavior ability includes the ability to gather and / or the ability to abandon corpses.

4. The use according to claim 1, characterized in that Fumigation is used to prevent and control 1-octen-3-ol.

5. The use according to claim 4, characterized in that The fumigation concentration of 1-octen-3-ol is greater than or equal to 0.0702 μg / cm3.

6. Application of 1-octen-3-ol in repelling red fire ants.

7. The use according to claim 6, characterized in that The concentration of 1-octen-3-ol is greater than or equal to 0.83 mg / mL.

8. Application of red fire ants killed by 1-octen-3-ol in the prevention and control of normal red fire ants.

9. The use according to claim 8, characterized in that The control of normal red fire ants is that the normal red fire ants give up cleaning the corpses of the red fire ants killed by 1-octen-3-ol, thereby controlling them.

10. The use according to claim 8 or 9, characterized in that: The concentration of 1-octen-3-ol in the red fire ants killed by 1-octen-3-ol is greater than or equal to 0.702 μg / cm3.

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