Pesticide composition containing flufenacet thioether and application of pesticide composition

Abstract

The invention belongs to the technical field of pesticides, and relates to a pesticide composition containing fluoromite thioether, which comprises an active component A and an active component B, the active ingredient A is flufenacet thioether, and the active ingredient B is selected from any one of spiromesifen and spirodiclofen diester. The pesticide composition or the preparation thereof can enhance the pesticide effect, expand the acaricidal spectrum, reduce the use cost, reduce the dosage, prolong the lasting period and delay the development of pesticide resistance.

Description

Technical Field

[0001] The invention relates to the technical field of pesticide compounding, and in particular to a pesticide composition containing fluazifop-containing chloranil and application thereof. Background Art

[0002] In recent years, with the rapid development of vegetable planting industry, vegetable varieties and production management conditions have been constantly changing, resulting in an increase in the types of vegetable diseases and pests. This has brought many inconveniences to vegetable planting and management, and also brought many uncertainties to the safety of vegetable consumption, posing a serious threat to human life and property safety.

[0003] The chemical name of fluazifop is (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl) sulfide, and its chemical structure is as follows:

[0004]

[0005] Spiromesifen's chemical name is [2-oxy-3-(2,4,6-trimethylphenyl)-1-oxaspiro[4,4]non-3-en-4-yl] 3,3-dimethylbutyrate. It is a spiro-tetra-keto acid insecticide and acaricide developed by Bayer. It has a unique spiro-tetra-keto acid structure, a novel mechanism of action and excellent insecticidal and acaricidal activity. It is mainly used in cotton, vegetables and ornamental plants to control piercing-sucking mouthparts pests. It is very effective against spider mites and whiteflies. The mechanism of action of spiromesifen is to affect the development of whiteflies and harmful mites, interfere with the biosynthesis of their liposomes, and at the same time, it can also close the ovarian tubes, reduce the reproductive capacity of whiteflies and harmful mites, and reduce the number of eggs laid.

[0006] The chemical name of spirobudiclib is dibutyl 3-(2,4-dichlorophenyl)-2-oxo-1-oxaspiro[4,5]dec-3-en-4-yl carbonate. Spirobudiclib is a non-systemic acaricide that controls eggs, nymphs and female adult mites through contact and stomach poisoning. Its mechanism of action is to inhibit fat synthesis in the body of harmful mites and block energy metabolism. It has no serious cross-resistance with conventional acaricides. Spirobudiclib has a good preventive effect on Panonychus, Tetranychus, Eotetranychus, and Tetranychus, and can be used to control mite pests in crops such as citrus, grapes, eggplants, and peppers.

[0007] In recent years, with the large-scale promotion of facility agriculture, mite pests have shown a trend of increasing year by year. Agricultural mites are small in size, reproduce quickly, have many generations, and have strong applicability. They are typical r-strategy explosive pests and are recognized as one of the most difficult to control harmful biological communities. Long-term continuous high-dose use of a single chemical agent can easily cause a series of problems such as drug resistance in mites and environmental pollution. Reasonable compounding or mixing of chemical agents has the positive characteristics of expanding the insecticide spectrum, improving the prevention and control effect, extending the appropriate period for application, reducing the amount of medicine used, reducing drug damage, reducing residues, and delaying the occurrence of pest resistance and resistance. The applicant was surprised to find through indoor toxicity experiments and field efficacy tests that the compound combination of fluazifop-thiophene and spirocyclopentyl or spiromesifen has a significant synergistic effect, and the compounded pesticide composition of fluazifop-thiophene and spirocyclopentyl or spiromesifen and its application have not been reported yet. Summary of the invention

[0008] Based on the above situation, the purpose of the present invention is to provide a pesticide composition containing fluazifop-sulfuron, which is mainly used for controlling phytophagous mites. The pesticide composition or its preparation can enhance the efficacy, reduce the dosage, and at the same time prolong the effective period and delay the development of drug resistance.

[0009] In order to achieve the above object, the present invention provides the following technical scheme: a pesticide composition containing fluazifop-sulfate, the pesticide composition comprising an active ingredient A and an active ingredient B, the active ingredient A is fluazifop-sulfate, and the active ingredient B is selected from any one of spiromesifen and spiroclofen;

[0010] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:28 to 30:1;

[0011] Furthermore, the mass ratio of fluazifop to spiromesifen is 1:28 to 30:1;

[0012] Furthermore, the mass ratio of fluazifop to spiromesifen is 1:28, 1:14, 1:8, 1:6, 2:3, 5:2, 8:1, 14:1, 20:1, 30:1;

[0013] Furthermore, the mass ratio of fluazifop to spiromesifen is 1:14 to 20:1;

[0014] Furthermore, the mass ratio of fluazifop to spiromesifen is 1:14, 1:8, 1:6, 2:3, 5:2, 8:1, 14:1, 20:1;

[0015] Furthermore, the mass ratio of fluazifop to spirobudicol is 1:28 to 28:1;

[0016] Furthermore, the mass ratio of fluazifop to spirobudicol is 1:28; 1:14; 1:8; 2:7; 1:2; 7:2; 14:1; 28:1;

[0017] Furthermore, the mass ratio of fluazifop to spirobudicol is 1:28 to 14:1;

[0018] Furthermore, the mass ratio of fluazifop to spirobudicol is 1:28; 1:14; 1:8; 2:7; 1:2; 7:2; 14:1;

[0019] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1 to 95 wt%,

[0020] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 5 to 80 wt%;

[0021] Furthermore, based on the total mass of the pesticide composition being 100 wt%, the sum of the contents of fluazifop and spiromesifen in the pesticide composition is 5 to 45 wt%;

[0022] Furthermore, based on the total mass of the pesticide composition as 100wt%, the sum of the contents of fluazifop and spiromesifen in the pesticide composition is 5wt%, 15wt%, 20wt%, 25wt%, 30wt%, or 45wt%;

[0023] Furthermore, based on the total mass of the pesticide composition being 100 wt%, the sum of the contents of fluazifop and spirobudicola in the pesticide composition is 5 to 45 wt%;

[0024] Furthermore, based on the total mass of the pesticide composition as 100wt%, the sum of the contents of fluazifop and spirobudicola in the pesticide composition is 5wt%, 15wt%, 20wt%, 25wt%, 30wt%, 35wt%, or 40wt%;

[0025] Furthermore, the pesticide composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers;

[0026] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0027] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0028] The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0029] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0030] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0031] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0032] The defoamer is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0033] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0034] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0035] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0036] The synergist is selected from synergist phosphite, synergist ether; and / or

[0037] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0038] The invention optimizes the content of active ingredients and auxiliary agents in the acaricide composition to achieve a better balance between toxicity and residue, thereby enhancing the efficacy, reducing the dosage and lowering the cost.

[0039] Furthermore, the pesticide composition can be prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation;

[0040] Furthermore, the solid preparation is selected from wettable powders and water dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, and suspensions;

[0041] The specific embodiments of the pesticide formulation prepared by the pesticide composition of the present invention are as follows:

[0042] When the pesticide composition is a wettable powder, the mass proportions of the components are: 1 to 40 parts of active ingredient A, 1 to 40 parts of active ingredient B, 3 to 10 parts of dispersant, 1 to 5 parts of wetting agent, filler, and deionized water to make up the balance;

[0043] The specific production steps of the wettable powder are as follows: the active ingredients, dispersants, wetting agents and fillers are mixed according to the above formula, uniformly stirred in a stirring tank, and mixed uniformly after air flow crushing to prepare the wettable powder of the composition of the present invention.

[0044] When the pesticide composition is a water-dispersible granule, the weight proportions of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 3-10 parts of dispersant, 1-10 parts of wetting agent, 1-5 parts of disintegrant, and the balance of filler;

[0045] The specific production steps of the water dispersible granules are as follows: the active ingredients and dispersants, wetting agents, disintegrants and fillers are uniformly mixed according to the above formula, crushed with an ultra-fine airflow mill, kneaded, and then added to a fluidized bed granulation dryer for granulation, drying, sieving, and sampling and analysis, thereby preparing the water dispersible granules of the composition of the present invention.

[0046] When the pesticide composition is an emulsifiable concentrate preparation, the weight proportions of the components are: 1 to 40 parts of active ingredient A, 1 to 40 parts of active ingredient B, 10 to 30 parts of conventional emulsifier, 20 to 50 parts of conventional solvent, and 1 to 5 parts of conventional synergist;

[0047] The specific production steps of the emulsifiable concentrate preparation are: firstly, the active ingredient is added into a solvent to be completely dissolved, then an emulsifier and a synergist are added, stirred evenly to form a uniform and transparent oily liquid, and then filled to prepare the emulsifiable concentrate preparation of the composition of the present invention.

[0048] When the pesticide composition is an aqueous emulsion, the components by weight are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 3-30 parts of emulsifier, 5-15 parts of solvent, 2-15 parts of stabilizer, 1-5 parts of antifreeze agent, 0.1-8 parts of defoamer, 0.2-2 parts of thickener, deionized water, and the balance;

[0049] The specific production steps of the aqueous emulsion are: adding the active ingredient, solvent, emulsifier and cosolvent together to dissolve into a uniform oil phase; mixing part of deionized water, antifreeze agent, antimicrobial agent and other pesticide adjuvants together to form a uniform water phase; adding the oil phase into the water phase while stirring at high speed in a reaction kettle, slowly adding water until the phase transition point is reached, turning on the shearing machine for high-speed shearing, adding the remaining water, and shearing for about half an hour to form an oil-in-water aqueous emulsion, thereby preparing the aqueous emulsion of the composition of the present invention.

[0050] When the pesticide composition is a microemulsion, the mass parts of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 10-30 parts of emulsifier, 1-8 parts of antifreeze agent, 0.5-10 parts of stabilizer, and 20-50 parts of conventional solvent cosolvent;

[0051] The specific production steps of the microemulsion are: dissolving the active ingredient completely with a cosolvent, adding other ingredients such as an emulsifier, an antifreeze stabilizer, etc., mixing evenly, and finally adding water, stirring sufficiently to prepare the microemulsion.

[0052] When the pesticide composition is a suspension, the components are as follows: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 1-10 parts of dispersant, 1-5 parts of antifreeze, 0.1-2 parts of thickener, 0.1-0.8 parts of defoamer, 0-10 parts of solvent, 1-5 parts of stabilizer, and the balance is made up of deionized water;

[0053] The specific production steps of the suspension are as follows: using glass beads, wet grinding with a sand mill until D90 (90% of the particle size) is less than 10 μm, and the suspension preparation of the composition of the present invention can be prepared.

[0054] Use of a pesticide composition containing fluazifop-sulphonate for preventing and controlling phytophagous mites;

[0055] Furthermore, the herbivorous mites are Tetranychus, Mylidae and / or Eupodidae pest mites;

[0056] Furthermore, the herbivorous pest mite is spider mite;

[0057] Furthermore, the spider mites are: Tetranychus truncatus, Tetranychus cinnabarinus, Panonychus citri, Tetranychus urticae;

[0058] Further, the pesticide composition and / or its preparation is applied at an effective dose to the harmful mites to be controlled or the medium in which they grow;

[0059] Compared with the prior art, the beneficial effects of the technical solution of the present invention are as follows:

[0060] (1) The compound pesticide of the present invention has a significant synergistic effect on the control effect;

[0061] (2) It is friendly to crops, non-target organisms, and the environment, increases the rapid effect of pesticides on pests, delays the development of pest resistance, and prolongs the effectiveness of pesticides;

[0062] (3) The compound pesticide of the present invention is compounded from two pesticides with different action mechanisms, has no cross-resistance, and is beneficial for delaying the development of pesticide resistance in mites. DETAILED DESCRIPTION

[0063] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0064] Indoor toxicity assay

[0065] Insect source for testing: Two-spotted spider mite (raised in the R&D center);

[0066] Test agents: 98% spiromesifen technical drug, 95% spiromesifen technical drug;

[0067] Preparation of test materials: Select female adult mites raised indoors and in the same physiological state. Cut double-sided tape into 2 cm long pieces and stick them on one end of the slide. Then select healthy mites and stick their backs on the double-sided tape. Put 30 mites per slide in a container padded with a wet sponge, cover it with a lid, and place it at (25±1)℃. After 2 hours, examine under a microscope, remove dead and injured individuals, and make up 30 mites per slide.

[0068] Preparation of drugs: The original drug was prepared into a mother solution with acetone, and the mother solution was prepared into test drugs with different proportions using 0.1% Tween-80 aqueous solution. The specific proportions and concentration gradients are as follows:

[0069] Table 1 Concentration gradients of fluazifop, spiromesifen and spiromesifen alone and in combination

[0070]

[0071]

[0072] Drug treatment: Immerse the slide in the drug solution and shake it gently for 5 seconds, then take it out, absorb the excess drug solution with absorbent paper, place it in a white porcelain plate with a wet sponge, cover it with a plastic film with good light transmittance, and place it at (25±1)℃ for observation. Each treatment is repeated 4 times, and a treatment without drug is set as a blank control.

[0073] Test investigation: 48 hours after treatment, check the death of test insects, record the total number of insects and the number of dead insects, and calculate the mortality rate:

[0074]

[0075] Where:

[0076] P——mortality rate, in percentage (%);

[0077] K——indicates the number of dead insects, in units of heads;

[0078] N——represents the total number of insects treated, in heads.

[0079]

[0080] Where:

[0081] P1——adjusted mortality rate, in percentage (%);

[0082] P t ——Treatment mortality rate, expressed as percentage (%);

[0083] P0——blank control mortality rate, in percentage (%).

[0084] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the above formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0085] The LC of each drug was calculated by linear regression analysis between the probability value of mortality and the logarithmic value of the series concentration. 50 The Sun Yunpei method was used to calculate the co-toxicity coefficient (CTC) of the mixture to evaluate the activity of the test agent against the test insects; the specific calculation formula is as follows:

[0086]

[0087] Where:

[0088] ATI——Actual Toxicity Index of Mixture;

[0089] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0090] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0091] TTI=TI A *P A +TI B *P B

[0092] Where:

[0093] TTI – Theoretical Toxicity Index of Mixtures;

[0094] TI A ——A: toxicity index of the agent;

[0095] P A ——The percentage of agent A in the mixture, in percentage (%);

[0096] TI B ——Toxicity index of agent B;

[0097] P B ——The percentage of agent B in the mixture, in percentage (%).

[0098]

[0099] Where:

[0100] CTC – Co-toxicity coefficient;

[0101] ATI——Actual Toxicity Index of Mixture;

[0102] TTI - Theoretical Toxicity Index of Mixture.

[0103] According to the classification standard of combined effects: a co-toxicity coefficient (CTC) ≥ 120 indicates a synergistic effect, a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect, and a co-toxicity coefficient (CTC) 80 < CTC < 120 indicates an additive effect.

[0104] Test results:

[0105] The combined toxicity test of fluazifop and spiromesifen at different ratios on Tetranychus urticae (female adult mites) is shown in Table 2. 50 The value is 4.504mg / L, and the LC value of spiromesifen against Tetranychus urticae is 50 The value is 7.313mg / L.

[0106] The control effect of fluazifop-sulphonate and spiromesifen on Tetranychus urticae was synergistic in the range of 1:28-30:1. When fluazifop-sulphonate and spiromesifen were 2:3, the co-toxicity coefficient was the highest, which was 198.202. LC 50 The value is 2.953mg / L.

[0107] Table 2 Indoor activity test results of fluazifop-sulphonate and spiromesifen against Tetranychus urticae (female adult mites)

[0108]

[0109]

[0110] The combined toxicity test of fluazifop-sulphonate and spiroclofenac at different ratios against Tetranychus urticae (female adult mites) is shown in Table 3. 50 The value is 15.873mg / L.

[0111] Fluometra thiocarbazone and spirobudiclofen showed synergistic effect on the control of Tetranychus urticae in the range of 1:28-28:1. When fluometra thiocarbazone and spirobudiclofen=1:2, the co-toxicity coefficient was the highest, which was 219.229, and LC 50 The value is 3.932mg / L.

[0112] Table 3 Indoor activity test results of fluazifop-sulphonate and spiroclofenac against Tetranychus urticae (female adult mites)

[0113]

[0114]

[0115] Preparation Example

[0116] Preparation Example 1

[0117] 5% Fluometraxin·Spiromethan EC (2+3)

[0118] Formula composition: Fluometra sulfide 2%, spiromesifen 3%, benzyl dimethylphenol polyoxyethyl ether 12%, sodium lauryl sulfate 6.5%, fatty alcohol polyoxyethylene ether 14%, soybean oil 5%, methyl oleate makes up the balance;

[0119] Preparation method: firstly add the active ingredient into the solvent and completely dissolve it, then add the emulsifier and the synergist, stir evenly to form a uniform and transparent oily liquid, and then fill it to prepare the emulsifiable concentrate preparation of the composition of the present invention.

[0120] Preparation Example 2

[0121] 15% Fluometra sulfothioate and Spiromesibiram suspension (5+10)

[0122] Formula composition: flumet sulfide 5%, spiromesifen 10%, alkyl naphthalene sulfonate formaldehyde condensate 3%, sodium lignin sulfonate 0.5%, polyether 3.5%, sodium lauryl sulfate 2%, silicone defoamer 0.5%, xanthan gum 0.2%, magnesium aluminum silicate 1%, ethylene glycol 4%, glycerol 2%, sodium benzoate 0.5%, deionized water to 100%;

[0123] Preparation method: Add active ingredients to the wetting dispersant and defoamer, use glass beads, and wet grind with a sand mill to D 90 (90% of the particle size) <10 μm, and a crushed slurry is obtained. A thickener, an antifreeze agent, and a preservative are added to the crushed slurry and mixed evenly, and deionized water is added to 100%, and a suspension is obtained by high-speed shearing.

[0124] Preparation Example 3

[0125] 40% Fluometra thiocarb · Spiromesibiram water dispersible granules (20+20)

[0126] Formula composition: fluazifop 20%, spiromesifen 20%, lignin sulfonate 8%, α-olefin sulfonate 2%, ammonium carbonate 2%, kaolin 20%, bentonite added to 100%;

[0127] Preparation method: first mix evenly, then grind to less than 15μm by air flow mill, mix evenly with water, granulate by rotary granulator, dry at 60℃ to a moisture content of ≤1%, select 20-mesh and 60-mesh test sieves for sieving, discard the large particles on the top and the dust filtered at the bottom, and prepare water-dispersible granules.

[0128] Preparation Example 4

[0129] 6% Fluometra thiocarb·Spiroclomethalin EC (2+4)

[0130] Formula composition: flumet sulfide 2%, spirobudic acid diester 4%, propylene carbonate 13%, sodium dodecylbenzene sulfonate 5.5%, fatty alcohol polyoxyethylene ether 10%, peanut oil 4%, methyl oleate makes up the balance;

[0131] Preparation method: firstly add the active ingredient into the solvent and completely dissolve it, then add the emulsifier and the synergist, stir evenly to form a uniform and transparent oily liquid, and then fill it to prepare the emulsifiable concentrate preparation of the composition of the present invention.

[0132] Preparation Example 5

[0133] 24% Fluometra sulfothioate and Spiromethoxaline Suspension (5+19)

[0134] Formula composition: flumet sulfide 5%, spirobudic acid diester 19%, alkyl naphthalene sulfonate formaldehyde condensate 3%, sodium lignin sulfonate 5%, EO-PO block copolymer 1%, sodium dodecyl sulfate 2%, silicone defoamer 0.5%, xanthan gum 0.2%, magnesium aluminum silicate 1%, ethylene glycol 3%, glycerol 2%, sodium benzoate 0.5%, deionized water to 100%;

[0135] Preparation method: Add active ingredients to the wetting dispersant and defoamer, use glass beads, and wet grind with a sand mill to D 90 (90% of the particle size) <10 μm, and a crushed slurry is obtained. A thickener, an antifreeze agent, and a preservative are added to the crushed slurry and mixed evenly, and deionized water is added to 100%, and a suspension is obtained by high-speed shearing.

[0136] Preparation Example 6

[0137] 40% fluazifop-succinate·spirobudicin wettable powder (15+25)

[0138] Formula composition: fluazifop 15%, spirobudic acid diester 25%, sodium lignin sulfonate 15%, sodium lauryl sulfate 3%, bentonite 6%, kaolin added to 100%;

[0139] Preparation method: pre-crush and mix evenly, then grind by air flow mill to a fineness that satisfies the requirement that at least 98wt% passes through a 45μm test sieve to obtain a wettable powder.

[0140] Field Examples

[0141] Field Example 1: Field Control of Strawberry Two-spotted Spider Mite

[0142] Test crops: Strawberry (Sweet Treasure);

[0143] Test subjects: Two-spotted spider mite;

[0144] Experimental design: The experiment set up 2 treatments of 5% fluazifop-sulphate·spiromesifen EC (2+3), 2 treatments of 15% fluazifop-sulphate·spiromesifen SC (5+10), 20% fluazifop-sulphate SC and 240 g / L spiromesifen SC as control treatments, and a water control. There were 7 treatments in total, 4 replicates, 28 plots, and the plot area was 25m 2 , using random block arrangement, with two rows of strawberries between each plot.

[0145] Table 4 Test agents and dosage

[0146]

[0147]

[0148] Experimental location and conditions: The experiment was set up at the strawberry base in Fei County, Linyi City, Shandong Province. The soil fertility of the experimental site is relatively high, the cultivation and management conditions are good, and it is planted all year round.

[0149] No watering was done during the test in 2021. It was cloudy on the day of application, with the highest temperature at 28°C, the lowest temperature at 15°C, the relative humidity at 52%, and the northeast wind at level 3. It was cloudy during the test.

[0150] No watering was done during the test in 2022. The weather was sunny on the day of application, with the highest temperature at 27°C, the lowest temperature at 15°C, the relative humidity at 55%, and the southeast wind at level 1. The weather was sunny during the test.

[0151] Application method, time and frequency: Use a backpack sprayer (3JWB16A model) to manually spray evenly on the front and back of the leaves. Spray the pesticide once on the morning of May 10, 2021 and April 23, 2022.

[0152] Survey method: Sampling was carried out at 5 points in each plot, 1 plant was fixed at each point, and 5 leaves with mites were fixed on each plant to investigate the insect population.

[0153] Survey time and frequency: Survey the insect population base before spraying, and survey once 1d, 7d, and 14d after spraying, for a total of 4 surveys.

[0154] Calculation method of drug efficacy:

[0155]

[0156]

[0157] During the experiment, it was observed that the strawberries in each treatment plot grew well, and no phytotoxicity was observed in any treatment.

[0158] The test results of each treatment agent for controlling strawberry two-spotted spider mite:

[0159] As can be seen from Table 5, the mixture of fluazifop-butyl and spiromesifen has a better control effect on two-spotted spider mites on strawberries. After 7 days of treatment with the active ingredient of 40 and 80 mg / kg, the control effect reached more than 80%.

[0160] During the control process using 5% fluazifop-sulphate-spiromesifen EC (2+3) and 15% fluazifop-sulphate-spiromesifen SC (5+10), the active ingredient was 80 mg / kg, and the control effect was above 80% 1 day after application, with good rapidity. 7 days after application, the control effect was above 94%, and the efficacy continued to increase. 14 days after application, the protective effect continued to increase. The protective effects of 5% fluazifop·spiromesifen EC (2+3) and 15% fluazifop·spiromesifen suspension (5+10), with an active ingredient of 40 mg / kg, reached more than 85%. When the active ingredient was 80 mg / kg, the protective effects were 90.14% and 91.28%, respectively. The efficacy of 5% fluazifop·spiromesifen EC (2+3) and 15% fluazifop·spiromesifen suspension (5+10) could last up to 14 days, indicating that the mixture of fluazifop and spiromesifen has a good persistent effect in controlling strawberry two-spotted spider mites.

[0161] Table 5 Effects of various treatments on the control of strawberry two-spotted spider mites (2021)

[0162]

[0163] Note: The protective effect (%) in the table is the average value of each replicate, and the difference is at the level of 0.01%, the same as Table 6 below.

[0164] As can be seen from Table 6, the combination of fluazifop and spiromesifen has a better control effect on two-spotted spider mites on strawberries. After 7 days of treatment with the active ingredient of 40 and 80 mg / kg, the control effect reached more than 80%. In the process of using 5% fluazifop and spiromesifen EC (2+3) and 15% fluazifop and spiromesifen SC (5+10), the active ingredient was 80 mg / kg, and the control effect was more than 80% 1 day after application, with good rapidity. 7 days after application, the control effect was more than 95%, and the efficacy continued to increase. 14 days after application, the protective effect continued to increase. The protective effects of 5% fluazifop-butyl·spiromesifen EC (2+3) and 15% fluazifop-butyl·spiromesifen suspension (5+10) with an active ingredient of 40 mg / kg reached more than 93%. When the active ingredient was 80 mg / kg, the protective effects were 100%. The efficacy of 5% fluazifop-butyl·spiromesifen EC (2+3) and 15% fluazifop-butyl·spiromesifen suspension (5+10) could last up to 14 days, indicating that the mixture of fluazifop-butyl and spiromesifen has a good persistent period in the control of strawberry two-spotted spider mites.

[0165] Compared with Table 5, the survey results on different days after application and the control efficacy of two-spotted spider mites under different concentrations of the agent are similar to the test results in 2021, indicating that the agent has good stability in preventing and controlling strawberry two-spotted spider mites.

[0166] Table 6 Effects of various treatments on the control of strawberry two-spotted spider mites (2022)

[0167]

[0168] Field Example 2: Field Control of Apple Two-spotted Spider Mite

[0169] Test crops: Apple (Red Fuji), row spacing 3m×3m;

[0170] Test subjects: Two-spotted spider mite;

[0171] Experimental design: The experiment set 6% fluazifop:spirobudic acid diester EC (2+4), 40% fluazifop:spirobudic acid diester WP (15+25), 20% fluazifop suspension, 24% spirobudic acid diester suspension as control treatment, and clear water control. A total of 5 treatments, 4 replicates, 20 plots, 2 trees per plot, randomized block arrangement, each plot separated by a row of fruit trees.

[0172] Table 7 Test agents and dosage

[0173]

[0174] Test location: apple orchard in Liangzhuang Town, Daiyue District, Tai'an City, Shandong Province. The growth of fruit trees in the test area is basically the same. The two-spotted spider mite has occurred more seriously in recent years. The orchard is located in a plain with clay loam.

[0175] The soil fertility of the experimental site is high, the cultivation and management conditions are good, and it is planted all year round.

[0176] No watering was done during the test in 2022. The pesticide was applied on June 7, 2022. It was sunny on the day of application, with the highest temperature of 29°C, the lowest temperature of 18°C, the relative humidity of 63%, and the southeast wind of level 2. The weather was cloudy and then sunny during the test.

[0177] Application method, time and frequency: Use a motorized sprayer (WL-ABSC model) and manually spray the whole tree evenly. The amount of application should be such that the leaves are evenly covered with the medicine and there is a little dripping. Apply the medicine once.

[0178] Survey method: Randomly select 5 branches with intact leaves from the east, south, west, north and center of each tree and mark them with signs. Count the number of live mites on all leaves. Survey 2 test trees in each plot. Survey once before and 2, 10 and 15 days after drug application.

[0179] Calculation method of drug efficacy: The drug efficacy is calculated according to the following formula:

[0180]

[0181]

[0182] During the experiment, apple trees in each experimental plot grew well and no pesticide damage was observed in any treatment.

[0183] As can be seen from Table 8, the mixed preparation of fluazifop-sulphonate and spirobudiclofen showed good rapid effect in controlling two-spotted spider mites on apples. The control effects of 6% fluazifop-sulphonate·spirobudiclofen emulsifiable concentrate (2+4) and 40% fluazifop-sulphonate·spirobudiclofen wettable powder (15+25) were 87.5% and 86.7%, respectively. At the 0.01 level, through difference analysis, the mixed preparation of fluazifop-sulphonate and spirobudiclofen was significantly higher than the control single-dose 20% fluazifop-sulphonate suspension and 24% spirobudiclofen suspension.

[0184] Table 8 Control effect of each treatment on apple two-spotted spider mite 2 days after application

[0185]

[0186] Note: The protective effect (%) in the table is the average value of each replicate, and the difference is at the level of 0.01%, the same as Table 9 and Table 10 below.

[0187] As can be seen from Table 9, the control effect of fluazifop-sulphonate and spirobudiclofen against two-spotted spider mites on apples improved with time. Ten days after application, the control effects of 6% fluazifop-sulphonate·spirobudiclofen emulsifiable concentrate (2+4) and 40% fluazifop-sulphonate·spirobudiclofen wettable powder (15+25) were 91.1% and 90.6%, respectively. At the 0.01 level, through difference analysis, the mixed preparation of fluazifop-sulphonate and spirobudiclofen was significantly higher than the control single-dose 20% fluazifop-sulphonate suspension and 24% spirobudiclofen suspension.

[0188] Table 9 Control effect of each treatment on apple two-spotted spider mite 10 days after application

[0189]

[0190] As can be seen from Table 10, the mixed preparation of fluazifop-sporin and spirobudiclofen showed very good persistence in the control of two-spotted spider mites on apples. The control effects of 6% fluazifop-sporin·spirobudiclofen emulsifiable concentrate (2+4) and 40% fluazifop-sporin·spirobudiclofen wettable powder (15+25) were 95.0% and 96.3%, respectively. At the 0.01 level, through difference analysis, the mixed preparation of fluazifop-sporin and spirobudiclofen was significantly higher than the control single-dose 20% fluazifop-sporin suspension and 24% spirobudiclofen suspension.

[0191] Table 10 The control effect of each treatment on apple two-spotted spider mite 15 days after the drug application

[0192]

[0193]

[0194] Through indoor toxicity determination and tests on strawberries and apples in the field, the acaricidal composition of the present invention, which is compounded with cyflumetofen and any one of spiromesifen and spiromesifen, shows good control effect on phytophagous mites. The acaricidal composition or its preparation obtained by compounding according to the present invention has significant control effect, and is superior to a single agent in delaying the generation of drug resistance and prolonging the lasting effect. In addition, no phytotoxicity of the compound agent to crops was found in the test, indicating that when the synergistic effect of killing mites of the obtained acaricidal composition or preparation is improved, the production cost and use cost can be reduced, and it is safe for crops.

[0195] The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different options and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A pesticide composition containing thiocarbazone, characterized in that: The pesticide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is fluazifop, and the active ingredient B is selected from any one of spiromesifen and spirobudicin.

2. The pesticide composition according to claim 1, characterized in that: The mass ratio of the active ingredient A to the active ingredient B is 1:28 to 30:

1.

3. The pesticide composition according to claim 1, characterized in that: The mass ratio of fluazifop to spiromesifen is 1:28 to 30:1, and the mass ratio of fluazifop to spiromesifen is 1:28 to 28:1; Preferably, the mass ratio of fluazifop to spiromesifen is 1:14 to 20:1; the mass ratio of fluazifop to spiromesifen is 1:28 to 14:

1.

4. The pesticide composition according to claim 1, characterized in that: Based on the total weight of the pesticide composition being 100 wt %, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1 to 95 wt %; Preferably, the sum of the contents of fluazifop and spiromesifen in the pesticide composition is 5 to 50 wt %; the sum of the contents of fluazifop and spiromesifen in the pesticide composition is 5 to 45 wt %.

5. The pesticide composition according to claim 1, characterized in that: In addition to the active ingredients, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

6. The pesticide composition according to claim 5, characterized in that: The pesticide composition can be prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation.

7. The pesticide composition according to claim 6, characterized in that: The solid preparation is selected from wettable powder and water dispersible granules; the liquid preparation is selected from emulsifiable concentrate, aqueous emulsion, microemulsion and suspension.

8. Use of the pesticide composition according to any one of claims 1 to 7 for controlling phytophagous mites.

9. The use according to claim 8, characterized in that: The herbivorous pest mites are spider mites cinnabarinus, spider mites two-spotted and spider mites truncate.

10. The use according to claim 8, characterized in that: The pesticide composition and / or its preparation is applied at an effective dose to the harmful mites to be controlled or the medium where they grow.

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