Pesticide composition containing clothianidin and application thereof

Abstract

The invention belongs to the technical field of pesticide disinfestation and sterilization, and discloses a clothianidin-containing pesticide composition and application thereof.The pesticide composition comprises a bactericide and an insecticide, the bactericide is pydiflumetofen and fludioxonil, the insecticide is clothianidin, and the insecticide is fludioxonil and fludioxonil. The pesticide composition can effectively prevent and control germs and pests underground and on the ground, solves the problems of poor prevention effect, high cost and serious pollution in a traditional prevention and control method, simplifies the disease and pest prevention and control process, reduces the labor intensity and cost of farmers, and improves the efficiency of agricultural production.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide insecticide and sterilization, and discloses a pesticide composition containing clothianidin and application thereof. Background Art

[0002] As the cultivation system and level of field agriculture continue to improve, the damage caused by soil-borne diseases of crops has become more serious year by year, becoming one of the factors that limit the growth and yield of crops. Although the existing new cultivation systems such as returning straw to the field and intercropping have the advantages of shortening the busy farming time, improving farming efficiency, reducing pollution, and reducing the cost of mechanical operations, they increase the incidence of diseases and cause the area of ​​soil-borne diseases of crops to continue to expand.

[0003] Seed coating technology plays an important role in the prevention and treatment of soil-borne diseases. Seed coating not only effectively suppresses the peak of disease before winter, but also forms a protective circle around the seeds in the soil. As the seeds grow and develop, the circle gradually expands horizontally and vertically, so that the root system is always in the protective circle, protecting the root system from infection by pathogens, thereby achieving the purpose of long-term control of soil-borne diseases. Therefore, screening out efficient seed coating agents has important guiding significance for the production of crops such as wheat, peanuts, and corn.

[0004] In recent years, with the development of seed treatment technology, some new seed dressing products with systemic fungicides as active ingredients have begun to be used to prevent and control soil-borne diseases. However, whether the commonly used agents in production can still have a good control effect on diseases after years of use in the field, and whether they can treat multiple diseases at the same time deserves further study; at the same time, whether the combination of agents with different mechanisms of action can treat multiple diseases at the same time and improve their control effect on diseases needs further discussion. Summary of the invention

[0005] Based on the above content, the purpose of the present invention is to provide a pesticide composition containing clothianidin, which can effectively prevent and control underground and above-ground pathogens and pests, and has a significant synergistic effect, solves the problems of poor prevention effect, high cost and serious pollution in traditional prevention and control methods, simplifies the pest control process, reduces farmers' labor intensity and cost, and improves the efficiency of agricultural production.

[0006] In order to achieve the above object, the present invention adopts the following technical scheme: a pesticide composition containing clothianidin, wherein the pesticide composition comprises a fungicide and an insecticide, wherein the fungicide is fludioxonil and fludioxonil, and the insecticide is clothianidin;

[0007] Furthermore, the mass ratio of fludioxonil to fludioxonil in the fungicide is 1:18 to 15:1, or any value within the above mass ratio range;

[0008] Furthermore, the mass ratio of fludioxonil to fludioxonil in the fungicide is 1:15 to 15:1, or any value within the above mass ratio range;

[0009] Furthermore, the mass ratio of fludioxonil to fludioxonil in the fungicide is 1:10 to 10:1, or any value within the above mass ratio range;

[0010] Furthermore, the mass ratio of the insecticide to the fungicide is 6:1 to 18:1, or any value within the above mass ratio range;

[0011] Furthermore, the mass ratio of the insecticide to the fungicide is 6:1 to 16:1, or any value within the above mass ratio range;

[0012] Furthermore, the mass ratio of the insecticide to the fungicide is 4:1 to 16:1, or any value within the above mass ratio range.

[0013] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the sum of the contents of the fungicides in the pesticide composition is 0.5% to 50%, or any value within the sum range of the above contents;

[0014] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the sum of the contents of the fungicides in the pesticide composition is 1% to 10%, or any value within the sum of the above contents.

[0015] Further, based on the total weight of the pesticide composition being 100 wt%, the content of the insecticide in the pesticide composition is 1% to 50%, or any value within the range of the sum of the above contents;

[0016] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the content of the insecticide in the pesticide composition is 5% to 35%, or any value within the sum of the above contents.

[0017] Furthermore, the pesticide composition also includes an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a warning color, a film-forming agent, a synergist and a carrier.

[0018] Furthermore, the pesticide composition is in the form of any one of a seed treatment suspension, a microcapsule suspension, and a dry seed dressing;

[0019] Furthermore, the pesticide composition is in the form of a seed treatment suspension.

[0020] The present invention also discloses the use of the above pesticide composition in preventing and controlling plant diseases and / or insect pests, wherein the plants include corn, wheat, rice or peanuts;

[0021] Further, the plant is peanut or wheat; the disease is any one of peanut root rot, peanut white rot, peanut stem rot, wheat stem base rot or wheat root rot;

[0022] Wheat root rot is a disease caused by the combined action of multiple fungi, which lurk in the soil and are difficult to detect in the early stages of the disease. The main pathogens are Cochliobolus graminearum, Helminthosporium graminearum, Fusarium spp., Helminthosporium graminearum, etc. Root rot is widely distributed and occurs in all wheat-growing countries. The pathogenic bacteria of root rot have a wide host range and can infect wheat and other gramineous crops and dozens of gramineous weeds. Root rot not only affects yield, but also reduces the quality and commercial value of wheat.

[0023] Wheat stem rot is a soil-borne fungal disease caused by Fusarium fungi that infects the base of wheat stems and can cause damage throughout the entire growing period. In the early stages of the disease, it causes seeds to fail to germinate normally, roots to rot, and leaves to turn yellow. As the disease progresses, the base of the stems becomes brown and easy to break in the middle and late stages, and red or white mold forms at the nodes, eventually causing white ears of wheat plants, unfilled grains or even seedless grains, greatly affecting wheat yields.

[0024] . Peanut root rot and white rot mainly harm the base of the peanut stem, the root pods and kernels, causing the death of the peanut plant. Various Fusarium spp. fungi are the main pathogens of peanut root rot, such as Fusarium solani, Fusarium oxysporum, Fusarium fujikura, Fusarium lamina, and Fusarium acuminatum. In addition, Aspergillus niger and Rhizopus arrhizus have also been reported to cause peanut root rot. The pathogen of peanut white rot is the genus Sclerotium of the order Asporales in the subphylum Ascomycota. The disease generally causes a yield loss of 10% to 30%, and in severe cases it can reach more than 80%.

[0025] The pest is any one of white grubs, cutworms, wireworms or aphids.

[0026] The beneficial effects of the present invention are as follows:

[0027] The pesticide composition of the present invention has reasonable components, can effectively prevent and control underground and above-ground pathogens and pests, and has a significant synergistic effect, solves the problems of poor prevention effect, high cost and serious pollution in traditional prevention and control methods, simplifies the pest control process, reduces farmers' labor intensity and cost, and improves the efficiency of agricultural production. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solutions and advantages of the present invention more concise and clear, the present invention is described with the following specific embodiments, but the present invention is by no means limited to these embodiments. The embodiments described below are only preferred embodiments of the present invention, which can be used to describe the present invention and cannot be understood as limiting the scope of the present invention. It should be pointed out that any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

[0029] Preparation process of seed treatment suspension: according to the ratio, the active ingredients, additives and water are mixed and stirred evenly through high shear, and then sanded by a sander for 2.5 hours to make the average particle size reach 1-5 microns. Finally, the thickener, preservative and film-forming agent are added and continued to be sheared and stirred evenly to obtain the seed treatment suspension.

[0030] Preparation Example 1: 7.5% clothianidin·fluoxetine·fludioxonil seed treatment suspension (6.25%+0.625%+0.625%) (10:1:1)

[0031] Formula composition: 6.25% clothianidin, 0.625% fluazifop, 0.625% fludioxonil, 2% alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate, 3% phenethylphenol polyoxyethylene polyoxypropylene ether, 2% fatty alcohol ethylene oxide-propylene oxide copolymer, 3% polycarboxylic acid sodium salt, 1% magnesium aluminum silicate, 0.25% xanthan gum, 1% polyacrylic acid emulsion, 6% rose red pigment, 5% propylene glycol, 0.5% silicone defoamer, 0.25% kason, and deionized water to make up the balance.

[0032] Comparative Example 1-1: 1.25% fludioxonil seed treatment suspension (0.625%+0.625%) (1:1)

[0033] Formula composition: 0.625% fluazifop-butyl, 0.625% fludioxonil, 2% alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate, 2% phenethylphenol polyoxyethylene polyoxypropylene ether, 1% fatty alcohol ethylene oxide-propylene oxide copolymer, 1% polycarboxylic acid sodium salt, 1% magnesium aluminum silicate, 0.25% xanthan gum, 0.5% polyacrylic acid emulsion, 4% rose red pigment, 4% propylene glycol, 0.5% silicone defoamer, 0.25% kason, and deionized water to make up the balance.

[0034] Comparative Example 1-2: 6.875% clothianidin·flufenacil seed treatment suspension (6.25%+0.625%) (10:1)

[0035] Formula composition: 6.25% clothianidin, 0.625% fluopicolide, 3% alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate, 3% phenethylphenol polyoxyethylene polyoxypropylene ether, 2% fatty alcohol ethylene oxide-propylene oxide copolymer, 1% polycarboxylic acid sodium salt, 1.5% magnesium aluminum silicate, 0.25% xanthan gum, 1% polyacrylic acid emulsion, 5% rose red pigment, 5% propylene glycol, 0.5% silicone defoamer, 0.25% kason, and deionized water to make up the balance.

[0036] Comparative Example 1-3: 6.875% clothianidin·fludioxonil seed treatment suspension (6.25%+0.625%) (10:1)

[0037] Formula composition: 6.25% clothianidin, 0.625% fludioxonil, 3% alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate, 2% phenethylphenol polyoxyethylene polyoxypropylene ether, 3% fatty alcohol ethylene oxide-propylene oxide copolymer, 1% polycarboxylic acid sodium salt, 2% magnesium aluminum silicate, 0.25% xanthan gum, 1% polyacrylic acid emulsion, 6% rose red pigment, 5% propylene glycol, 0.5% silicone defoamer, 0.25% kason, and deionized water to make up the balance.

[0038] Preparation Example 2: 13.5% clothianidin·fluoxetine·fludioxonil seed treatment suspension (12%+0.5%+1%) (24:1:2)

[0039] Formula composition: 12% clothianidin, 0.5% fluazifop-hydroxylamide, 1% fludioxonil, 3% sorbitan oleate polyoxyethylene ether, 1% sodium polycarboxylate, 2% phenethylphenol polyoxyethylene polyoxypropylene ether, 2% fatty alcohol polyoxyethylene ether sodium sulfate, 1.5% magnesium aluminum silicate, 0.25% xanthan gum, 1% polyacrylic acid emulsion, 5% rose red pigment, 5% propylene glycol, 0.5% silicone defoamer, 0.3% benzisothiazolinone potassium, and deionized water to make up the balance.

[0040] Comparative Example 2-1: 1.5% fluazifop-dipamide·fludioxonil seed treatment suspension (0.5%+1%) (1:2)

[0041] Formula composition: 0.5% fluazifop-butyl, 1% fludioxonil, 1% sorbitan oleate polyoxyethylene ether, 2% sodium polycarboxylate, 1% phenethylphenol polyoxyethylene polyoxypropylene ether, 2% fatty alcohol polyoxyethylene ether sodium sulfate, 1% magnesium aluminum silicate, 0.25% xanthan gum, 1% polyacrylic acid emulsion, 4% rose red pigment, 3% propylene glycol, 0.5% silicone defoamer, 0.15% potassium benzisothiazolinone, and deionized water to make up the balance.

[0042] Comparative Example 2-2: 12.5% ​​clothianidin·flufenacil seed treatment suspension (12%+0.5%) (24:1)

[0043] Formula composition: 12% clothianidin, 0.5% fluopicolide, 2% sorbitan oleate polyoxyethylene ether, 2% sodium polycarboxylate, 3% phenethylphenol polyoxyethylene polyoxypropylene ether, 1% fatty alcohol polyoxyethylene ether sodium sulfate, 1% magnesium aluminum silicate, 0.25% xanthan gum, 1% polyacrylic acid emulsion, 6% rose red pigment, 5% propylene glycol, 0.5% silicone defoamer, 0.2% benzisothiazolinone potassium, and deionized water to make up the balance.

[0044] Comparative Example 2-3: 13% clothianidin·fludioxonil seed treatment suspension (12%+1%) (12:1)

[0045] Formula composition: 12% clothianidin, 1% fludioxonil, 3% sorbitan oleate polyoxyethylene ether, 1% sodium polycarboxylate, 1% phenethylphenol polyoxyethylene polyoxypropylene ether, 1% fatty alcohol polyoxyethylene ether sodium sulfate, 1.5% magnesium aluminum silicate, 0.25% xanthan gum, 1% polyacrylic acid emulsion, 5% rose red pigment, 5% propylene glycol, 0.5% silicone defoamer, 0.3% potassium benzisothiazolinone, and deionized water to make up the balance.

[0046] Preparation Example 3: 13% clothianidin·fluoxetine·fludioxonil seed treatment suspension (12%+0.4%+0.6%) (60:2:3)

[0047] Formula composition: 12% clothianidin, 0.4% fluazifop, 0.6% fludioxonil, 2% ethylene glycol oxyethylene polyoxypropylene ether, 2% aromatic phenol polyoxyethylene ether phosphate, 2% sodium lauryl sulfate, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 6% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0048] Comparative Example 3-1: 1% fluazifop-dipamide·fludioxonil seed treatment suspension (0.4%+0.6%) (2:3)

[0049] Formula composition: 0.4% fluazifop-butyl, 0.6% fludioxonil, 1% ethylene glycol oxyethylene polyoxypropylene ether, 1% aromatic phenol polyoxyethylene ether phosphate, 2% sodium lauryl sulfate, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 5% rose red pigment, 4% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0050] Comparative Example 3-2: 12.4% clothianidin·flufenacil seed treatment suspension (12%+0.4%) (30:1)

[0051] Formula composition: 12% clothianidin, 0.4% fluopicolide, 2% ethylene glycol oxyethylene polyoxypropylene ether, 2% aromatic phenol polyoxyethylene ether phosphate, 1% sodium lauryl sulfate, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 5% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0052] Comparative Example 3-3: 12.6% clothianidin·fludioxonil seed treatment suspension (12%+0.6%) (20:1)

[0053] Formula composition: 12% clothianidin, 0.6% fludioxonil, 2% ethylene glycol oxyethylene polyoxypropylene ether, 2% aromatic phenol polyoxyethylene ether phosphate, 3% sodium dodecyl sulfate, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 5% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0054] Preparation Example 4: 7% clothianidin·fluoxetine·fludioxonil seed treatment suspension (6%+0.8%+0.2%) (30:4:1)

[0055] Formula composition: 6% clothianidin, 0.8% fluazifop-hydroxylamide, 0.2% fludioxonil, 3% tristyrylphenol ethoxylate phosphate, 5% EO / PO block copolymer, 2% Guerbet alcohol polyoxyethylene ether, 2% alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate, 1% polyacrylic acid, 0.25% xanthan gum, 6% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% potassium benzoate, and deionized water to make up the balance.

[0056] Comparative Example 4-1: 1% fluazifop-dipamide·fludioxonil seed treatment suspension (0.8%+0.2%) (4:1)

[0057] Formula composition: 0.8% fluazifop-butyl hydroxylamide, 0.2% fludioxonil, 2% tristyrylphenol ethoxylate phosphate, 3% EO / PO block copolymer, 2% Guerbet alcohol polyoxyethylene ether, 1% alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate, 1% polyacrylic acid, 0.25% xanthan gum, 5% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 0.7% potassium benzoate, and deionized water to make up the balance.

[0058] Comparative Example 4-2: 6.8% clothianidin·flufenacil seed treatment suspension (6%+0.8%) (15:2)

[0059] Formula composition: 6% clothianidin, 0.8% fluopicolide, 3% tristyrylphenol ethoxylate phosphate, 2% EO / PO block copolymer, 3% Guerbet alcohol polyoxyethylene ether, 2% alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate, 1% polyacrylic acid, 0.25% xanthan gum, 5% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 0.9% potassium benzoate, and deionized water to make up the balance.

[0060] Comparative Example 4-3: 6.2% clothianidin·fludioxonil seed treatment suspension (6%+0.2%) (30:1)

[0061] Formula composition: 6% clothianidin, 0.2% fludioxonil, 2% tristyrylphenol ethoxylate phosphate, 2% EO / PO block copolymer, 3% Guerbet alcohol polyoxyethylene ether, 3% alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate, 1% polyacrylic acid, 0.25% xanthan gum, 5% rose red pigment, 4% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 0.8% potassium benzoate, and deionized water to make up the balance.

[0062] Example 1: Indoor combined action activity assay for wheat root rot

[0063] The target pathogen for the test: Bipolaris sorokiniana, was isolated, purified, identified and preserved by the laboratory of the Bioassay Center.

[0064] Test agents: Fluoxetine hydroxylamide technical, fludioxonil technical and clothianidin technical.

[0065] Test method: Using the mycelium growth method, the above original drugs were dissolved in a suitable solvent, and then diluted with a 0.1% Tween 80 aqueous solution to prepare single-dose mother liquors. Different ratios were prepared by experimental design. Each single dose and each group of mixed ratios were prepared into the required series of mass concentrations. The test agent was evenly mixed with the sterilized PDA culture medium, prepared into different concentration gradients, poured into a culture dish (9 cm in diameter) to prepare a drug-containing culture medium plate, and sterile water was used as a control. Each concentration was repeated 4 times. A bacterial cake was taken from the edge of the pre-cultured Sorberia colony, picked out and placed in the middle of the PDA plate, and cultured in the dark at 28°C for 7 to 10 days. The colony diameter was measured by the cross method, the inhibition rate was calculated, and the data was processed and analyzed by DPS to obtain the EC 50 value.

[0066] Calculation method: According to the survey results, calculate the mycelial growth inhibition rate of each treatment concentration on the target bacteria for the test. The unit is percentage (%). The calculation result is rounded to two decimal places.

[0067] D=D1-D2

[0068] Where:

[0069] D——colony growth diameter;

[0070] D1——colony diameter;

[0071] D2——diameter of mushroom cake.

[0072]

[0073] Where:

[0074] I——hyphae growth inhibition rate;

[0075] D0——blank control colony growth diameter;

[0076] D T ——The growth diameter of the colony after treatment with chemicals.

[0077] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of ≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; and 80<CTC<120 indicates an additive effect.

[0078] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0079]

[0080] Where:

[0081] ATI——Actual Toxicity Index of Mixture;

[0082] S——EC of standard fungicide 50 , the unit is milligrams per liter (mg / L);

[0083] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0084] TTI=TI A ×P A +TI B ×P B

[0085] Where:

[0086] TTI – Theoretical Toxicity Index of Mixtures;

[0087] TI A ——A: toxicity index of the agent;

[0088] P A ——The percentage of agent A in the mixture, in percentage (%);

[0089] TIB ——Toxicity index of agent B;

[0090] P B ——The percentage of agent B in the mixture, in percentage (%).

[0091]

[0092] Where:

[0093] CTC – Co-toxicity coefficient;

[0094] ATI——Actual Toxicity Index of Mixture;

[0095] TTI - Theoretical Toxicity Index of Mixture.

[0096] The test results are shown in the following table:

[0097] Table 1 Results of indoor bioassay of fludioxonil, fludioxonil and their mixed agents against Soxhletia solani

[0098]

[0099] The test results show that, as shown in Table 1, the combination of fluopicolide and fludioxonil has a good control effect on the mycelial growth of Helminthosporium sorbii. Fluopicolide and fludioxonil show synergistic effects in the mass ratio range of 1:15 to 15:1; when the mass ratio of fluopicolide and fludioxonil is 1:10 to 10:1, the co-toxicity coefficient of fluopicolide to Helminthosporium sorbii is greater than 130, and the synergistic effect is obvious; among them, the activity is better when the mass ratio of fluopicolide:fludioxonil is 1:2, and the co-toxicity coefficient is 181.757.

[0100] Table 2 Results of indoor toxicity test on Helminthosporium sorborifolium of the mixture of fludioxonil and fludioxonil in a ratio of 1:1 and its mixture with clothianidin

[0101] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin:fludioxonil (1:1) y=1.5324x+5.3644 0.5783 / Clothianidin:flutrifloxystrobin:fludioxonil=4:1:1 y=1.5028x+5.4496 0.5021 115.176 Clothianidin:fluoxetine:fludioxonil=8:1:1 y=1.4720x+5.5056 0.4534 127.547 Clothianidin:fluoxetine:fludioxonil=10:1:1 y=1.6040x+5.6334 0.4028 143.570 Clothianidin:fluoxetine:fludioxonil=18:1:1 y=1.4544x+5.5218 0.4378 132.092 Clothianidin:flutriclostrobin:fludioxonil=30:1:1 y=1.5919x+5.5417 0.4568 126.598 Clothianidin:fluoxetine:fludioxonil=36:1:1 y=1.5314x+5.4892 0.4792 120.680 Clothianidin:fluoxetine:fludioxonil=40:1:1 y=1.5909x+5.389 0.5695 101.545

[0102] Note: Clothianidin had no significant inhibitory activity against Helminthosporium sorboriformis at the designed highest concentration of 200 mg / L, and EC could not be calculated. 50 and virulence regression equations.

[0103] From the indoor test results in Table 2, it can be seen that the mass ratio of "fluoxathiapyronil + fludioxonil" mixed with clothianidin at 1:1 showed a certain inhibitory effect on the mycelial growth of Sorbaria. When the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapyronil + fludioxonil) was 4:1-18:1, it showed a synergistic effect on Sorbaria; the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapyronil + fludioxonil) was (5-9):1, it had a significant synergistic effect on Sorbaria. Among them, when the mass ratio of clothianidin:fluopoxadone:fluthrin was (8-36):1:1, it showed a synergistic effect on Helicobacter pylori; when the mass ratio of clothianidin:fluopoxadone:fluthrin was (10-18):1:1, it had a significant synergistic effect on Helicobacter pylori; and the mass ratio of clothianidin:fluopoxadone:fluthrin was 4:1:1 and 40:1:1, it showed an additive effect on Helicobacter pylori.

[0104] Table 3 Results of indoor toxicity test on Helminthosporium sorborifolium of the mixture of fludioxonil and fludioxonil in a ratio of 1:2 and its mixture with clothianidin

[0105] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin:fludioxonil (1:2) y=1.5515x+5.2237 0.7175 / Clothianidin:flutriclostrobin:fludioxonil=3:1:2 y=1.535x+5.1423 0.8078 88.821 Clothianidin:flutriclostrobin:fludioxonil=6:1:2 y=1.5305x+5.3823 0.5627 127.510 Clothianidin:fluoxetine:fludioxonil=15:1:2 y=1.5143x+5.4031 0.5418 132.429 Clothianidin:fluoxetine:fludioxonil=24:1:2 y=1.471x+5.4128 0.5241 136.901 Clothianidin:flutrifloxystrobin:fludioxonil=30:1:2 y=1.4593x+5.4004 0.5317 134.945 Clothianidin:flutriclostrobin:fludioxonil=45:1:2 y=1.4841x+5.3877 0.5480 130.931 Clothianidin:flutriclostrobin:fludioxonil=54:1:2 y=1.5757x+5.3251 0.6219 115.372

[0106] Note: Clothianidin had no significant inhibitory activity against Helminthosporium sorboriformis at the designed highest concentration of 200 mg / L, and EC could not be calculated. 50 and virulence regression equations.

[0107] From the indoor test results in Table 3, it can be seen that the mass ratio of "fluoxathiapyronil + fludioxonil" mixed with clothianidin at 1:2 showed a certain inhibitory effect on the mycelial growth of Sorbaria. When the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapyronil + fludioxonil) was 2:1-15:1, it showed a synergistic effect on Sorbaria; the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapyronil + fludioxonil) was (5-10):1, it had a significant synergistic effect on Sorbaria. Among them, when the mass ratio of clothianidin:fluopoxadone:fluthrin was (6-45):1:2, it showed a synergistic effect on Helicobacter pylori; when the mass ratio of clothianidin:fluopoxadone:fluthrin was (15-30):1:2, it had a significant synergistic effect on Helicobacter pylori; and the mass ratio of clothianidin:fluopoxadone:fluthrin was 3:1:2 and 54:1:2, it showed an additive effect on Helicobacter pylori.

[0108] Example 2: Indoor combined action activity assay for wheat stem rot

[0109] The target pathogen for the test: F. pseudograminearum, was isolated, purified, identified and preserved by the laboratory of the Bioassay Center.

[0110] Test agents: Fluoxetine hydroxylamide technical, fludioxonil technical and clothianidin technical.

[0111] Experimental method: The mycelium growth rate method was used to determine the effect of each test agent on the growth of Fusarium graminearum. The original drug was dissolved in a suitable solvent and then diluted with a 0.1% Tween 80 aqueous solution to prepare a single-dose stock solution. Different ratios were prepared by experimental design. Each single dose and each group of mixed agents were prepared into the required series of mass concentrations. Each test agent was mixed with the sterilized PDA medium in a certain proportion to prepare a series of drug-containing PDA plates with a concentration gradient. At the same time, a sterilized puncher was used to prepare the test mycelium blocks from the fresh Fusarium graminearum strains cultured on PDA, and they were inoculated in the center of each test drug-containing PDA plate with the mycelium facing down. The blank control was a PDA medium without drugs. Each treatment was repeated 4 times. After culturing at 25°C for 7 to 10 days, the mycelium diameter was measured by the cross method, and the mycelium growth inhibition rate was calculated. The DPS statistical analysis system was used to calculate the EC of each test agent on Fusarium graminearum. 50 And other data.

[0112] Calculation method: According to the survey results, calculate the mycelial growth inhibition rate of each treatment concentration on the target bacteria for the test. The unit is percentage (%). The calculation result is rounded to two decimal places.

[0113] D=D1-D2

[0114] Where:

[0115] D——colony growth diameter;

[0116] D1——colony diameter;

[0117] D2——diameter of mushroom cake.

[0118]

[0119] Where:

[0120] I——hyphae growth inhibition rate;

[0121] D0——blank control colony growth diameter;

[0122] D T ——The growth diameter of the colony after treatment with chemicals.

[0123] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of ≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; and 80<CTC<120 indicates an additive effect.

[0124] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0125]

[0126] Where:

[0127] ATI——Actual Toxicity Index of Mixture;

[0128] S——EC of standard fungicide 50 , the unit is milligrams per liter (mg / L);

[0129] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0130] TTI=TI A ×P A +TI B ×P B

[0131] Where:

[0132] TTI – Theoretical Toxicity Index of Mixtures;

[0133] TI A ——A: toxicity index of the agent;

[0134] P A ——The percentage of agent A in the mixture, in percentage (%);

[0135] TI B ——Toxicity index of agent B;

[0136] P B ——The percentage of agent B in the mixture, in percentage (%).

[0137]

[0138] Where:

[0139] CTC – Co-toxicity coefficient;

[0140] ATI——Actual Toxicity Index of Mixture;

[0141] TTI - Theoretical Toxicity Index of Mixture.

[0142] The test results are shown in the following table:

[0143] Table 4 Indoor bioassay results of fluopicolide, fludioxonil and their mixed agents against Fusarium graminearum

[0144]

[0145] The test results show that, as shown in Table 4, the combination of fluopicolide and fludioxonil has a good control effect on the mycelial growth of F. graminearum. The mass ratio of fluopicolide to fludioxonil is 1:20-12:1, which shows a synergistic effect on F. graminearum; the mass ratio of fluopicolide to fludioxonil is 1:18-6:1, and the co-toxicity coefficient of F. graminearum is greater than 130, and the synergistic effect is obvious; among them, the activity is better when the mass ratio of fluopicolide to fludioxonil is 2:3, and the co-toxicity coefficient is 198.142.

[0146] Table 5 Results of indoor toxicity test on Fusarium graminearum of the mixture of fluopicolide + fludioxonil in a ratio of 1:1 and its mixture with clothianidin

[0147] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin:fludioxonil (1:1) y=1.601x+6.8095 0.0741 / Clothianidin:flutrifloxystrobin:fludioxonil=4:1:1 y=1.6017x+6.8378 0.0712 104.073 Clothianidin:fluoxetine:fludioxonil=8:1:1 y=1.7187x+7.1656 0.0550 134.727 Clothianidin:fluoxetine:fludioxonil=10:1:1 y=1.4884x+6.9404 0.0497 149.095 Clothianidin:fluoxetine:fludioxonil=18:1:1 y=1.6063x+7.0642 0.0519 142.775 Clothianidin:flutrifloxystrobin:fludioxonil=30:1:1 y=1.578x+6.9819 0.0555 133.514 Clothianidin:flutrifloxystrobin:fludioxonil=36:1:1 y=1.638x+6.9838 0.0615 120.488 Clothianidin:fluoxetine:fludioxonil=40:1:1 y=1.564x+6.7099 0.0807 91.822

[0148] Note: Clothianidin had no significant inhibitory activity against Fusarium graminearum at the designed maximum concentration of 200 mg / L, and the EC could not be calculated. 50 and virulence regression equations.

[0149] From the indoor test results in Table 5, it can be seen that the mass ratio of "fluoxathiapiprolin + fludioxonil" mixed with clothianidin at 1:1 showed a certain inhibitory effect on the growth of F. graminearum. When the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapiprolin + fludioxonil) was 4:1-18:1, it showed a synergistic effect on F. graminearum; the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapiprolin + fludioxonil) was (5-9):1, it had a significant synergistic effect on F. graminearum. Among them, when the mass ratio of clothianidin:fluopoxadone:fluthrin was (8-36):1:1, it showed a synergistic effect on Fusarium graminearum; when the mass ratio of clothianidin:fluopoxadone:fluthrin was (10-18):1:1, it had a significant synergistic effect on Fusarium graminearum; and the mass ratio of clothianidin:fluopoxadone:fluthrin was 4:1:1 and 40:1:1, it showed an additive effect on Fusarium graminearum.

[0150] Table 6 Results of indoor toxicity test on Fusarium graminearum of the mixture of fluopicolide + fludioxonil in a ratio of 2:3 and its mixture with clothianidin

[0151] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin:fludioxonil (2:3) y=1.6169x+6.8222 0.0746 / Clothianidin:flutriclostrobin:fludioxonil=10:2:3 y=1.5358x+6.6825 0.0803 92.902 Clothianidin:flutriclostrobin:fludioxonil=20:2:3 y=1.6464x+6.8883 0.0713 104.628 Clothianidin:flutriclostrobin:fludioxonil=30:2:3 y=1.6733x+7.0655 0.0583 127.959 Clothianidin:flutriclostrobin:fludioxonil=40:2:3 y=1.7072x+7.1445 0.0554 134.657 Clothianidin:flutriclostrobin:fludioxonil=60:2:3 y=1.6067x+7.0835 0.0505 147.723 Clothianidin:flutrifloxystrobin:fludioxonil=70:2:3 y=1.564x+6.9738 0.0547 136.380 Clothianidin:fluoxetine:fludioxonil=80:2:3 y=1.4999x+6.8254 0.0607 122.900

[0152] Note: Clothianidin had no significant inhibitory activity against Fusarium graminearum at the designed maximum concentration of 200 mg / L, and the EC could not be calculated. 50 and virulence regression equations.

[0153] From the indoor test results in Table 6, it can be seen that the mass ratio of "fluoxathiapiprolin + fludioxonil" mixed with clothianidin at 2:3 showed a certain inhibitory effect on the growth of F. graminearum. When the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapiprolin + fludioxonil) was 6:1-16:1, it showed a synergistic effect on F. graminearum; the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapiprolin + fludioxonil) was (8-14):1, it had a significant synergistic effect on F. graminearum. Among them, when the mass ratio of clothianidin:fluopoxadone:fluthrin was (30-80):2:3, it showed a synergistic effect on Fusarium graminearum; when the mass ratio of clothianidin:fluopoxadone:fluthrin was (40-70):2:3, it had a significant synergistic effect on Fusarium graminearum; and when the mass ratio of clothianidin:fluopoxadone:fluthrin was 10:2:3, it showed an additive effect on Fusarium graminearum.

[0154] Example 3: Indoor joint activity determination of peanut white rot

[0155] The test pathogen: Sclerotium rolfsii Sacc, was isolated, purified, identified and preserved by the laboratory of the Bioassay Center.

[0156] Test agents: Fluoxetine hydroxylamide technical, fludioxonil technical and clothianidin technical.

[0157] Test method: The mycelium growth rate method was used to determine the virulence of the pathogen. The original drug was dissolved in a suitable solvent, and then diluted with a 0.1% Tween 80 aqueous solution to prepare a single-dose stock solution. Different ratios were prepared by experimental design. Each single dose and each group of mixed ratios were prepared into the required series of mass concentrations. The test agent was added to the PDA medium that was pre-dissolved and cooled to about 50°C, shaken well, and drug-containing plates with different concentration gradients were prepared. An equal amount of sterile water was added to the PDA medium as a blank control. Take the cultured peanut white rot strain, use a sterilized puncher to punch out the bacterial cake, and transfer the bacterial cake to the center of the toxic culture medium. Each agent was treated with 5 different concentrations, with a total of 4 repetitions. Cultured at a constant temperature of 28°C, when the control colony was close to filling the culture dish, the "cross" method was used to measure the colony diameter under each treatment, and the average value was calculated to obtain the inhibition rate. The DPS statistical analysis system was used to calculate the EC of each test agent for the whole sclerotium. 50 And other data.

[0158] Calculation method: According to the survey results, calculate the mycelial growth inhibition rate of each treatment concentration on the target bacteria for the test. The unit is percentage (%). The calculation result is rounded to two decimal places.

[0159] D=D1-D2

[0160] Where:

[0161] D——colony growth diameter;

[0162] D1——colony diameter;

[0163] D2——diameter of mushroom cake.

[0164]

[0165] Where:

[0166] I——hyphae growth inhibition rate;

[0167] D0——blank control colony growth diameter;

[0168] D T ——The growth diameter of the colony after treatment with chemicals.

[0169] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of ≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; and 80<CTC<120 indicates an additive effect.

[0170] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0171]

[0172] Where:

[0173] ATI——Actual Toxicity Index of Mixture;

[0174] S——EC of standard fungicide 50 , the unit is milligrams per liter (mg / L);

[0175] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0176] TTI=TI A ×P A +TI B ×P B

[0177] Where:

[0178] TTI – Theoretical Toxicity Index of Mixtures;

[0179] TI A ——A: toxicity index of the agent;

[0180] P A ——The percentage of agent A in the mixture, in percentage (%);

[0181] TI B——Toxicity index of agent B;

[0182] P B ——The percentage of agent B in the mixture, in percentage (%).

[0183]

[0184] Where:

[0185] CTC – Co-toxicity coefficient;

[0186] ATI——Actual Toxicity Index of Mixture;

[0187] TTI - Theoretical Toxicity Index of Mixture.

[0188] The test results are shown in the following table:

[0189] Table 7 Results of indoor bioassay of fluopicolide, fludioxonil and their composite mixtures on Sclerotium sclerotiorum

[0190]

[0191] As shown in Table 7, the combination of fluopicolide and fludioxonil has a good inhibitory effect on the mycelial growth of Sclerotium sclerotium. The mass ratio of fluopicolide to fludioxonil is 1:12-16:1, which shows a synergistic effect on Sclerotium sclerotium; the mass ratio of fluopicolide to fludioxonil is 1:9-12:1, and the co-toxicity coefficient of Sclerotium sclerotium is greater than 130, and the synergistic effect is obvious; among them, the activity is better when the mass ratio of fluopicolide to fludioxonil is 1:1, and the co-toxicity coefficient is 177.274.

[0192] Table 8 Results of indoor toxicity test on Sclerotium sclerotiorum in the mixture of fludioxonil and fludioxonil in a ratio of 1:1 and its mixture with clothianidin

[0193] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin:fludioxonil (1:1) y=1.5475x+5.5434 0.4455 / Clothianidin:flutrifloxystrobin:fludioxonil=4:1:1 y=1.7643x+5.5229 0.5054 88.148 Clothianidin:flutriclostrobin:fludioxonil=5:1:1 y=1.5269x+5.6039 0.4023 110.738 Clothianidin:fluoxetine:fludioxonil=6:1:1 y=1.5414x+5.6745 0.3651 122.021 Clothianidin:fluoxetine:fludioxonil=8:1:1 y=1.5594x+5.7521 0.3294 135.246 Clothianidin:fluoxetine:fludioxonil=10:1:1 y=1.4675x+5.7733 0.2972 149.899 Clothianidin:fluoxetine:fludioxonil=12:1:1 y=1.5363x+5.7899 0.3061 145.541 Clothianidin:fluoxetine:fludioxonil=18:1:1 y=1.4362x+5.7069 0.3220 138.354 Clothianidin:flutriclostrobin:fludioxonil=20:1:1 y=1.3669x+5.636 0.3425 130.073 Clothianidin:flutrifloxystrobin:fludioxonil=30:1:1 y=1.4684x+5.6731 0.3480 128.017 Clothianidin:flutrifloxystrobin:fludioxonil=36:1:1 y=1.3991x+5.6028 0.3708 120.146

[0194] Note: At the designed highest concentration of 200 mg / L, clothianidin had no significant inhibitory activity against Sclerotium sclerotiorum, and EC could not be calculated. 50 and virulence regression equations.

[0195] From the indoor test results in Table 8, it can be seen that the mixture of "fluoxathiapiprolin + fludioxonil" with a mass ratio of 1:1 and clothianidin showed a certain inhibitory effect on the growth of mycelium of Sclerotium sclerotium. When the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapiprolin + fludioxonil) was 3:1-18:1, the synergistic effect of Sclerotium sclerotium was shown; the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapiprolin + fludioxonil) was (5-9):1, which had a significant synergistic effect on Sclerotium sclerotium. Among them, when the mass ratio of clothianidin:fluopoxadone:fluthrin was (6-36):1:1, it showed a synergistic effect on the whole Sclerotium; when the mass ratio of clothianidin:fluopoxadone:fluthrin was (10-12):1:1, it had a significant synergistic effect on the whole Sclerotium; and when the mass ratio of clothianidin:fluopoxadone:fluthrin was 4:1:1 and 5:1:1, it showed an additive effect on the whole Sclerotium.

[0196] Table 9 Results of indoor toxicity test on Sclerotium sclerotiorum of the mixture of fludioxonil and fludioxonil in a ratio of 4:1 and its mixture with clothianidin

[0197] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin:fludioxonil (4:1) y=1.5564x+5.1679 0.7801 / Clothianidin:flutrifloxystrobin:fludioxonil=5:4:1 y=1.4075x+5.1464 0.7871 99.111 Clothianidin:fluoxetine:fludioxonil=10:4:1 y=1.5519x+5.1872 0.7575 102.983 Clothianidin:fluoxetine:fludioxonil=15:4:1 y=1.4589x+5.2422 0.6823 114.334 Clothianidin:fluoxetine:fludioxonil=20:4:1 y=1.4010x+5.2656 0.6463 120.702 Clothianidin:flutrifloxystrobin:fludioxonil=30:4:1 y=1.3812x+5.2869 0.6198 125.863 Clothianidin:flutriclostrobin:fludioxonil=40:4:1 y=1.4164x+5.3363 0.5788 134.779 Clothianidin:flutrifloxystrobin:fludioxonil=50:4:1 y=1.5377x+5.3443 0.5972 130.626 Clothianidin:flutrifloxystrobin:fludioxonil=60:4:1 y=1.4003x+5.3026 0.6080 128.306 Clothianidin:flutrifloxystrobin:fludioxonil=70:4:1 y=1.3987x+5.2701 0.6410 121.700 Clothianidin:flutriclostrobin:fludioxonil=75:4:1 y=1.5038x+5.2354 0.6974 111.858

[0198] Note: At the designed highest concentration of 200 mg / L, clothianidin had no significant inhibitory activity against Sclerotium sclerotiorum, and EC could not be calculated. 50 and virulence regression equations.

[0199] From the indoor test results in Table 9, it can be seen that the mixture of "fluoxathiapiprolin + fludioxonil" with a mass ratio of 4:1 and clothianidin showed a certain inhibitory effect on the growth of mycelium of Sclerotium sclerotium. When the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapiprolin + fludioxonil) was 4:1-14:1, the synergistic effect of Sclerotium sclerotium was shown; the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapiprolin + fludioxonil) was (8-10):1, which had a significant synergistic effect on Sclerotium sclerotium. Among them, when the mass ratio of clothianidin:fluopoxadone:fluthrin was (20-70):4:1, it showed a synergistic effect on the whole Sclerotium; when the mass ratio of clothianidin:fluopoxadone:fluthrin was (40-50):4:1, it had a significant synergistic effect on the whole Sclerotium; and the mass ratio of clothianidin:fluopoxadone:fluthrin was 5:4:1, 10:4:1, 15:4:1, and 75:4:1, it showed an additive effect on the whole Sclerotium.

[0200] Example 4: Indoor joint activity assay for peanut root rot

[0201] The test pathogen: Fusarium solani, was isolated, purified, identified and preserved by the laboratory of the Bioassay Center.

[0202] Test agents: Fluoxetine hydroxylamide technical, fludioxonil technical and clothianidin technical.

[0203] Test method: The test adopts the mycelium growth rate inhibition method. Select a suitable solvent to dissolve the original drug, and then dilute it with a 0.1% Tween 80 aqueous solution to prepare a single-dose mother liquor, and prepare different ratios according to the experimental design. Each single dose and each group of mixed ratios are prepared into the required series of mass concentrations. Add 75 ml of dissolved PDA culture medium respectively, so that the agent and PDA culture medium are fully mixed and then directly poured into a sterilized culture dish to prepare a drug-containing PDA plate of the corresponding concentration (with the treatment of adding an equal amount of sterile water as a control). Inoculate the pathogen cake in the center of the plate, and each agent and control are repeated 4 times, and cultured at a constant temperature of 27°C. According to the growth of mycelium in the control dish, the cross-cross method is used to measure the colony growth diameter of each treatment, calculate the mycelium growth amount and relative inhibition rate, and analyze and compare the antibacterial effects of different agent treatments on the mycelium of the test pathogens. The DPS statistical analysis system is used to calculate the EC of each test agent on the whole sclerotium. 50 And other data.

[0204] Calculation method: According to the survey results, calculate the mycelial growth inhibition rate of each treatment concentration on the target bacteria for the test. The unit is percentage (%). The calculation result is rounded to two decimal places.

[0205] D=D1-D2

[0206] Where:

[0207] D——colony growth diameter;

[0208] D1——colony diameter;

[0209] D2——diameter of mushroom cake.

[0210]

[0211] Where:

[0212] I——hyphae growth inhibition rate;

[0213] D0——blank control colony growth diameter;

[0214] D T ——The growth diameter of the colony after treatment with chemicals.

[0215] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of ≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; and 80<CTC<120 indicates an additive effect.

[0216] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0217]

[0218] Where:

[0219] ATI——Actual Toxicity Index of Mixture;

[0220] S——EC of standard fungicide 50 , the unit is milligrams per liter (mg / L);

[0221] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0222] TTI=TI A ×P A +TI B ×P B

[0223] Where:

[0224] TTI – Theoretical Toxicity Index of Mixtures;

[0225] TI A ——A: toxicity index of the agent;

[0226] P A ——The percentage of agent A in the mixture, in percentage (%);

[0227] TI B ——Toxicity index of agent B;

[0228] P B ——The percentage of agent B in the mixture, in percentage (%).

[0229]

[0230] Where:

[0231] CTC – Co-toxicity coefficient;

[0232] ATI——Actual Toxicity Index of Mixture;

[0233] TTI - Theoretical Toxicity Index of Mixture.

[0234] The test results are shown in the following table:

[0235] Table 10 Results of indoor bioassay of fluopicolide, fludioxonil and their mixed mixtures against Fusarium solani

[0236]

[0237] As shown in Table 10, the combination of fluopicolide and fludioxonil has a good control effect on the mycelial growth of Fusarium solani. The mass ratio of fluopicolide to fludioxonil is 1:20-15:1, which shows a synergistic effect; the mass ratio of fluopicolide to fludioxonil is 1:18-10:1, and the co-toxicity coefficient of Fusarium solani is greater than 130, and the synergistic effect is obvious; among them, the activity is better when the mass ratio of fluopicolide to fludioxonil is 1:3, and its co-toxicity coefficient is 198.154.

[0238] Table 11 Results of indoor toxicity test on Fusarium solani of the mixture of fludioxonil and fludioxonil in a ratio of 1:1 and its mixture with clothianidin

[0239] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin:fludioxonil (1:1) y=1.6099x+5.4938 0.4935 / Clothianidin:flutrifloxystrobin:fludioxonil=4:1:1 y=1.5412x+5.5158 0.4627 106.657 Clothianidin:flutriclostrobin:fludioxonil=5:1:1 y=1.7848x+5.6953 0.4078 121.015 Clothianidin:fluoxetine:fludioxonil=6:1:1 y=1.7355x+5.7751 0.3576 138.003 Clothianidin:fluoxetine:fludioxonil=8:1:1 y=1.7124x+5.7969 0.3425 144.088 Clothianidin:fluoxetine:fludioxonil=10:1:1 y=1.7483x+5.8438 0.3291 149.954 Clothianidin:fluoxetine:fludioxonil=12:1:1 y=1.7781x+5.8527 0.3315 148.869 Clothianidin:fluoxetine:fludioxonil=18:1:1 y=1.6471x+5.7023 0.3741 131.917 Clothianidin:flutriclostrobin:fludioxonil=20:1:1 y=1.3776x+5.5629 0.3903 126.441 Clothianidin:flutrifloxystrobin:fludioxonil=30:1:1 y=1.3532x+5.4896 0.4347 113.527 Clothianidin:flutrifloxystrobin:fludioxonil=36:1:1 y=1.3790x+5.3598 0.5484 89.989

[0240] Note: Clothianidin has no significant inhibitory activity against Fusarium solani at the designed maximum concentration of 200 mg / L, and EC cannot be calculated 50 and virulence regression equations.

[0241] From the indoor test results in Table 11, it can be seen that the mixture of "flupyraclostrobin + fludioxonil" with a mass ratio of 1:1 and clothianidin showed a certain inhibitory effect on the mycelial growth of Fusarium solani. When the mass ratio of the insecticide clothianidin to the fungicide (flupyraclostrobin + fludioxonil) was 5:2-18:1, it showed a synergistic effect on Fusarium solani; the mass ratio of the insecticide clothianidin to the fungicide (flupyraclostrobin + fludioxonil) was (4-6):1, which had a significant synergistic effect on Fusarium solani. Among them, when the mass ratio of clothianidin:fluopoxadone:fluthrin was (5-20):1:1, it showed a synergistic effect on Fusarium solani; when the mass ratio of clothianidin:fluopoxadone:fluthrin was (8-12):1:1, it had a significant synergistic effect on Fusarium solani; and the mass ratio of clothianidin:fluopoxadone:fluthrin was 4:1:1, 30:1:1, and 36:1:1, it showed an additive effect on Fusarium solani.

[0242] Table 12 Results of indoor toxicity test on Fusarium solani of the mixture of fluopicolide + fludioxonil in a ratio of 1:3 and its mixture with clothianidin

[0243] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin:fludioxonil (1:3) y=1.5443x+5.3388 0.6034 / Clothianidin:flutrifloxystrobin:fludioxonil=4:1:3 y=1.4427x+5.2691 0.6509 92.702 Clothianidin:fluazifop-dipamide:fludioxonil=8:1:3 y=1.4249x+5.3703 0.5497 109.769 Clothianidin:fluoxetine:fludioxonil=12:1:3 y=1.4776x+5.4653 0.4843 124.592 Clothianidin:fluoxetine:fludioxonil=16:1:3 y=1.7067x+5.5640 0.4672 129.152 Clothianidin:flutriclostrobin:fludioxonil=20:1:3 y=1.5939x+5.5613 0.4444 135.779 Clothianidin:fluoxetine:fludioxonil=24:1:3 y=1.3791x+5.5222 0.4181 144.320 Clothianidin:flutriclostrobin:fludioxonil=32:1:3 y=1.5371x+5.5382 0.4465 135.140 Clothianidin:flutriclostrobin:fludioxonil=40:1:3 y=1.7104x+5.5571 0.4724 127.731 Clothianidin:fluoxetine:fludioxonil=48:1:3 y=1.6930x+5.5366 0.4820 125.187 Clothianidin:flutrifloxystrobin:fludioxonil=60:1:3 y=1.4413x+5.3313 0.5890 102.445

[0244] Note: Clothianidin has no significant inhibitory activity against Fusarium solani at the designed maximum concentration of 200 mg / L, and EC cannot be calculated 50 and virulence regression equations.

[0245] From the indoor test results in Table 12, it can be seen that the mass ratio of "flupyraclostrobin + fludioxonil" mixed with clothianidin at 1:3 showed a certain inhibitory effect on the mycelial growth of Fusarium solani. When the mass ratio of the insecticide clothianidin to the fungicide (flupyraclostrobin + fludioxonil) was 3:1-12:1, it showed a synergistic effect on Fusarium solani; the mass ratio of the insecticide clothianidin to the fungicide (flupyraclostrobin + fludioxonil) was (5-8):1, it had a significant synergistic effect on Fusarium solani. Among them, when the mass ratio of clothianidin:fluopoxadone:fluthrin was (12-48):1:3, it showed a synergistic effect on Fusarium solani; when the mass ratio of clothianidin:fluopoxadone:fluthrin was (20-32):1:3, it had a significant synergistic effect on Fusarium solani; and the mass ratio of clothianidin:fluopoxadone:fluthrin was 4:1:3, 8:1:3, and 60:1:3, it showed an additive effect on Fusarium solani.

[0246] Example 5: Indoor joint toxicity assay of aphids

[0247] Test pests: Schizaphis graminum, which are raised long-term in the biological testing laboratory. The breeding conditions are: temperature 22-25°C, relative humidity 60%-70%, and light-dark ratio of 14h:10h in an insect breeding room, and no contact with pesticides during the period.

[0248] Test method: Indoor toxicity determination adopts the immersion method. The original drug is dissolved in a suitable solvent, and then diluted with a 0.1% Tween 80 aqueous solution. The single-dose mother liquor is prepared respectively, and different ratios are prepared by experimental design. Each single dose and each group of mixed ratios are prepared into the required series of mass concentrations. Aphids of the same size are selected on wheat as test insects. The wheat leaves with aphids are immersed in different concentrations of the agent for 5 to 10 seconds, taken out and dried on newspapers, and then transferred to a disposable plastic cup covered with 2% agar. The results are checked after 24 hours. The death standard is that the insect body does not move when touched lightly with a brush. Each treatment is repeated 4 times, and each repeat is 20 test insects. 0.1% Tween-80 is used as a blank control.

[0249] Calculation method:

[0250] According to the survey data, the adjusted mortality rate of each treatment was calculated in percentage (%); the specific calculation formula is as follows:

[0251]

[0252] Where:

[0253] P——mortality rate, in percentage (%);

[0254] K——indicates the number of dead insects, in units of heads;

[0255] N——represents the total number of insects treated, in heads.

[0256]

[0257] P1——adjusted mortality rate, in percentage (%);

[0258] P t ——Treatment mortality rate, expressed as percentage (%);

[0259] P0——blank control mortality rate, in percentage (%).

[0260] If the control mortality rate is less than 5%, no correction is required; if the mortality rate is between 5% and 20%, correction should be made according to the above formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0261] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0262]

[0263] Where:

[0264] ATI——Actual Toxicity Index of Mixture;

[0265] S——LC of standard reagent 50 , the unit is milligrams per liter (mg / L);

[0266] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0267] TTI=TI A ×P A +TI B ×P B

[0268] Where:

[0269] TTI – Theoretical Toxicity Index of Mixtures;

[0270] TI A ——A: toxicity index of the agent;

[0271] P A ——The percentage of agent A in the mixture, in percentage (%);

[0272] TI B ——Toxicity index of agent B;

[0273] P B ——The percentage of agent B in the mixture, in percentage (%).

[0274]

[0275] Where:

[0276] CTC – Co-toxicity coefficient;

[0277] ATI——Actual Toxicity Index of Mixture;

[0278] TTI - Theoretical Toxicity Index of Mixture.

[0279] The co-toxicity coefficient of the combination CTC≥120 shows a synergistic effect; CTC≤80 shows an antagonistic effect; 80<CTC<120 shows an additive effect.

[0280] The results of the indoor biological activity test are as follows:

[0281] Table 13 Results of indoor toxicity test on Schizaphis graminis by 1:1 mixture of fludioxonil + fludioxonil and its mixture with clothianidin

[0282] Test drug Virulence regression equation <![CDATA[LC 50 (mg.L -1 )]]> Co-toxicity coefficient Clothianidin y=1.5509x+4.0439 4.1350 / Clothianidin:flutrifloxystrobin:fludioxonil=4:1:1 y=1.5984x+4.0395 3.9894 103.650 Clothianidin:fluoxetine:fludioxonil=8:1:1 y=1.7667x+4.0667 3.3751 122.515 Clothianidin:fluoxetine:fludioxonil=10:1:1 y=1.7515x+4.1385 3.1038 133.224 Clothianidin:fluoxetine:fludioxonil=18:1:1 y=1.6960x+4.1476 3.1811 129.986 Clothianidin:flutrifloxystrobin:fludioxonil=30:1:1 y=1.6168x+4.1636 3.2608 126.809 Clothianidin:flutrifloxystrobin:fludioxonil=36:1:1 y=1.6941x+4.0991 3.4023 121.535 Clothianidin:fluoxetine:fludioxonil=40:1:1 y=1.4832x+4.0362 4.4649 92.611

[0283] Note: The mixture of fludioxonil and chlorpyrifos in a ratio of 1:1 had no significant activity against Schizaphis graminis at the designed maximum concentration of 200 mg / L, and the LC cannot be calculated. 50 and virulence regression equations.

[0284] The results of indoor activity test in Table 13 show that the mixture of fludioxonil and fludioxonil has no obvious activity against Schizaphis graminis. Clothianidin has good insecticidal activity against Schizaphis graminis, and its LC 50 4.1350mg.L -1 . Compounding the mixture of fluopicolide and fludioxonil with clothianidin in a reasonable ratio into a ternary compound preparation can significantly increase the toxicity of clothianidin, enhance the control effect on aphids, and significantly reduce the dosage. When the mass ratio of the insecticide clothianidin to the fungicide (fluopicolide + fludioxonil) is 4:1 to 18:1, it shows a synergistic effect on the aphid; the mass ratio of the insecticide clothianidin to the fungicide (fluopicolide + fludioxonil) is (5 to 9):1, it has a significant synergistic effect on the aphid. Among them, the mass ratio of clothianidin:fluopoxadone:fludioxonil is (8-36):1:1, which shows a synergistic effect on aphids; the mass ratio of clothianidin:fluopoxadone:fludioxonil is (10-18):1:1, which has a significant synergistic effect on aphids; and the mass ratio of clothianidin:fluopoxadone:fludioxonil is 4:1:1 and 40:1:1, which shows an additive effect on Schizaphis graminis.

[0285] Table 14 Results of indoor toxicity test on Schizaphis graminis by the mixture of fludioxonil and fludioxonil in the ratio of 2:3 and its mixture with clothianidin

[0286] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Clothianidin y=1.5509x+4.0439 4.1350 / Clothianidin:flutriclostrobin:fludioxonil=10:2:3 y=1.7461x+3.9702 3.8885 106.339 Clothianidin:flutriclostrobin:fludioxonil=20:2:3 y=1.5945x+4.1492 3.4165 121.030 Clothianidin:flutriclostrobin:fludioxonil=30:2:3 y=1.5053x+4.2498 3.1504 131.253 Clothianidin:flutriclostrobin:fludioxonil=40:2:3 y=1.6553x+4.1812 3.1238 132.371 Clothianidin:flutriclostrobin:fludioxonil=60:2:3 y=1.5764x+4.2008 3.2133 128.684 Clothianidin:flutrifloxystrobin:fludioxonil=70:2:3 y=1.7901x+4.0731 3.2944 125.516 Clothianidin:fluoxetine:fludioxonil=80:2:3 y=1.7168x+4.0906 3.3861 122.117

[0287] Note: The mixture of fludioxonil and chlorpyrifos in a ratio of 2:3 had no significant activity against Schizaphis graminis at the designed maximum concentration of 200 mg / L, and the LC cannot be calculated. 50 and virulence regression equations.

[0288] The results of indoor activity test in Table 14 show that the mass ratio of the insecticide clothianidin to the fungicide (flutriazole + fludioxonil) is 4:1-16:1, which shows a synergistic effect on the aphid; the mass ratio of the insecticide clothianidin to the fungicide (flutriazole + fludioxonil) is (6-12):1, which has a significant synergistic effect on the aphid. The mass ratio of clothianidin:flutriazole:fludioxonil is (20-80):2:3, which shows a synergistic effect on aphids; the mass ratio of clothianidin:flutriazole:fludioxonil is (30-60):2:3, which has a significant synergistic effect on aphids; and the mass ratio of clothianidin:flutriazole:fludioxonil is 10:2:3, which shows an additive effect on the aphid.

[0289] Table 15 Indoor toxicity test results of fluopicolide + fludioxonil in a ratio of 2:3 and its mixture with clothianidin against Schizaphis graminis

[0290] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Clothianidin y=1.5509x+4.0439 4.1350 / Clothianidin:flutriclostrobin:fludioxonil=3:1:2 y=1.6013x+3.9809 4.3291 95.516 Clothianidin:flutriclostrobin:fludioxonil=6:1:2 y=1.6449x+3.9991 4.0593 101.865 Clothianidin:fluoxetine:fludioxonil=15:1:2 y=1.8037x+4.0935 3.1812 129.982 Clothianidin:fluoxetine:fludioxonil=24:1:2 y=1.4970x+4.2569 3.1359 131.860 Clothianidin:flutrifloxystrobin:fludioxonil=30:1:2 y=1.6619x+4.1550 3.2244 128.241 Clothianidin:flutriclostrobin:fludioxonil=45:1:2 y=1.5008x+4.2220 3.2993 125.330 Clothianidin:flutriclostrobin:fludioxonil=54:1:2 y=1.6262x+4.1325 3.4155 121.066

[0291] Note: The mixture of fludioxonil and chlorpyrifos in a ratio of 1:2 had no significant activity against Schizaphis graminis at the designed maximum concentration of 200 mg / L, and the LC cannot be calculated. 50 and virulence regression equations.

[0292] The results of indoor activity test in Table 15 show that when the mass ratio of the insecticide clothianidin to the fungicide (flutriazole + fludioxonil) is 5:1-17:1, it shows a synergistic effect on the aphid; the mass ratio of the insecticide clothianidin to the fungicide (flutriazole + fludioxonil) is (5-10):1, which has a significant synergistic effect on the aphid. The mass ratio of clothianidin:flutriazole:fludioxonil is (15-54):1:2, which shows a synergistic effect on aphids; the mass ratio of clothianidin:flutriazole:fludioxonil is (15-30):1:1, which has a significant synergistic effect on aphids; and the mass ratio of clothianidin:flutriazole:fludioxonil is 3:1:2 and 6:1:2, which shows an additive effect on the aphid.

[0293] Example 6: Indoor joint action toxicity assay of grubs

[0294] Test target: Holotrichia parallela Motschulsky, 2nd instar larvae reared indoors were selected.

[0295] Test agents: Fluoxetine hydroxylamide, fludioxonil, and clothianidin technical drugs.

[0296] Test method: The toxicity of each agent to grubs was determined by the immersion method. The original drug was dissolved in a suitable solvent, and then diluted with a 0.1% Tween 80 aqueous solution to prepare a single-dose stock solution. Different ratios were prepared by experimental design. Each single agent and each group of mixed agents were prepared into the required series of mass concentrations. The test grubs were soaked in the liquid for 10 seconds and dried, and then placed in a plastic cup with 100g of sterilized soil in each cup. One insect was raised in each cup, and several germinated peanut seeds were placed in the cup as feed. They were raised at 25°C. 20 grubs were used for each treatment, and repeated 4 times. The results were checked after 72 hours. The number of dead insects was recorded.

[0297] Calculation method:

[0298] According to the survey data, the adjusted mortality rate of each treatment was calculated in percentage (%); the specific calculation formula is as follows:

[0299]

[0300] Where:

[0301] P——mortality rate, in percentage (%);

[0302] K——indicates the number of dead insects, in units of heads;

[0303] N——represents the total number of insects treated, in heads.

[0304]

[0305] P1——adjusted mortality rate, in percentage (%);

[0306] P t ——Treatment mortality rate, expressed as percentage (%);

[0307] P0——blank control mortality rate, in percentage (%).

[0308] If the control mortality rate is less than 5%, no correction is required; if the mortality rate is between 5% and 20%, correction should be made according to the above formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0309] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0310]

[0311] Where:

[0312] ATI——Actual Toxicity Index of Mixture;

[0313] S——LC of standard reagent 50, the unit is milligrams per liter (mg / L);

[0314] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0315] TTI=TI A ×P A +TI B ×P B

[0316] Where:

[0317] TTI – Theoretical Toxicity Index of Mixtures;

[0318] TI A ——A: toxicity index of the agent;

[0319] P A ——The percentage of agent A in the mixture, in percentage (%);

[0320] TI B ——Toxicity index of agent B;

[0321] P B ——The percentage of agent B in the mixture, in percentage (%).

[0322]

[0323] Where:

[0324] CTC – Co-toxicity coefficient;

[0325] ATI——Actual Toxicity Index of Mixture;

[0326] TTI - Theoretical Toxicity Index of Mixture.

[0327] The co-toxicity coefficient of the combination CTC≥120 shows a synergistic effect; CTC≤80 shows an antagonistic effect; 80<CTC<120 shows an additive effect.

[0328] The results of the indoor biological activity test are as follows:

[0329] Table 16 Results of indoor toxicity test on dark beetles of fluopicolide + fludioxonil in a 1:1 ratio and its mixture with clothianidin

[0330] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Clothianidin y=1.5616x+3.0320 18.2065 / Clothianidin:fluoxetine:fludioxonil=6:1:1 y=1.4159x+3.3382 14.9183 122.041 Clothianidin:fluoxetine:fludioxonil=8:1:1 y=1.3488x+3.4771 13.4630 135.234 Clothianidin:fluoxetine:fludioxonil=10:1:1 y=1.3341x+3.5403 12.4211 146.577 Clothianidin:fluoxetine:fludioxonil=12:1:1 y=1.1738x+3.6939 12.9629 140.451 Clothianidin:fluoxetine:fludioxonil=18:1:1 y=1.9183x+2.8289 13.5460 134.405 Clothianidin:flutriclostrobin:fludioxonil=20:1:1 y=1.3928x+3.3821 14.5102 125.474 Clothianidin:flutrifloxystrobin:fludioxonil=30:1:1 y=1.8272x+2.7436 17.1751 106.005

[0331] Note: The mixture of fludioxonil and fludioxonil in a ratio of 1:1 had no significant activity against the dark beetle at the designed maximum concentration of 200 mg / L, and the LC cannot be calculated. 50 and virulence regression equations.

[0332] The results of indoor activity test in Table 16 show that the mixture of fludioxonil and fludioxonil has no obvious activity against the dark scarab beetle. Clothianidin has good insecticidal activity against the dark scarab beetle, and its LC 50 18.2065mg.L -1 . Compounding the mixture of fluopicolide and fludioxonil with clothianidin in a reasonable ratio into a ternary compound preparation can significantly increase the toxicity of clothianidin, enhance the control effect on white grubs, and significantly reduce the dosage. When the mass ratio of the insecticide clothianidin to the fungicide (fluopicolide + fludioxonil) is 3:1 to 10:1, it shows a synergistic effect on the dark beetle; the mass ratio of the insecticide clothianidin to the fungicide (fluopicolide + fludioxonil) is (4 to 9):1, it has a significant synergistic effect on the dark beetle. Among them, the mass ratio of clothianidin:fluopoxadone:fludioxonil is (6-20):1:1, which shows a synergistic effect on the dark beetle; the mass ratio of clothianidin:fluopoxadone:fludioxonil is (8-18):1:1, which has a significant synergistic effect on white grubs; and the mass ratio of clothianidin:fluopoxadone:fludioxonil is 30:1:1, which shows an additive effect on the dark beetle.

[0333] Table 17 Results of indoor toxicity test on dark beetles of fluopicolide + fludioxonil in a ratio of 4:1 and its mixture with clothianidin

[0334] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Clothianidin y=1.5616x+3.032 18.2065 / Clothianidin:fluoxetine:fludioxonil=15:4:1 y=1.7041x+2.9947 15.0219 121.200 Clothianidin:fluoxetine:fludioxonil=20:4:1 y=1.5802x+3.1992 13.7909 132.018 Clothianidin:flutrifloxystrobin:fludioxonil=30:4:1 y=1.4086x+3.4385 12.8398 141.797 Clothianidin:flutriclostrobin:fludioxonil=40:4:1 y=1.5464x+3.2684 13.1769 138.170 Clothianidin:flutrifloxystrobin:fludioxonil=50:4:1 y=1.6343x+3.1172 14.1941 128.268 Clothianidin:flutrifloxystrobin:fludioxonil=60:4:1 y=1.6820x+3.0296 14.8401 122.684 Clothianidin:flutriclostrobin:fludioxonil=75:4:1 y=1.7292x+2.7756 19.3373 94.152

[0335] Note: The mixture of fludioxonil and fludioxonil in a ratio of 1:1 had no significant activity against the dark beetle at the designed maximum concentration of 200 mg / L, and the LC cannot be calculated. 50 and virulence regression equations.

[0336] The results of indoor activity determination in Table 17 show that when the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapyronil + fludioxonil) is 3:1-12:1, a synergistic effect is exhibited on the dark scarab beetle; the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapyronil + fludioxonil) is (4-8):1, which has a significant synergistic effect on the dark scarab beetle. The mass ratio of clothianidin:fluoxathiapyronil:fludioxonil is (15-60):4:1, which has a synergistic effect on the dark scarab beetle; the mass ratio of clothianidin:fluoxathiapyronil:fludioxonil is (20-40):4:1, which has a significant synergistic effect on the grub; and the mass ratio of clothianidin:fluoxathiapyronil:fludioxonil is 75:4:1, which has an additive effect on the dark scarab beetle.

[0337] Table 18 Results of indoor toxicity test on dark beetles of fluopicolide + fludioxonil in a ratio of 1:3 and its mixture with clothianidin

[0338] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Clothianidin y=1.5616x+3.0320 18.2065 / Clothianidin:fluoxetine:fludioxonil=12:1:3 y=1.6791x+3.0345 14.8114 122.922 Clothianidin:fluoxetine:fludioxonil=16:1:3 y=1.4359x+3.3857 13.3135 136.752 Clothianidin:flutriclostrobin:fludioxonil=20:1:3 y=1.5061x+3.3344 12.7615 142.667 Clothianidin:fluoxetine:fludioxonil=24:1:3 y=1.5552x+3.2950 12.4838 145.841 Clothianidin:flutriclostrobin:fludioxonil=32:1:3 y=1.6087x+3.2003 13.1451 138.504 Clothianidin:flutriclostrobin:fludioxonil=40:1:3 y=1.5014x+3.2657 14.2932 127.379 Clothianidin:flutrifloxystrobin:fludioxonil=60:1:3 y=1.8347x+2.7613 16.6040 109.651

[0339] Note: The mixture of fludioxonil and fludioxonil in a ratio of 1:1 had no significant activity against the dark beetle at the designed maximum concentration of 200 mg / L, and the LC cannot be calculated. 50 and virulence regression equations.

[0340] The results of indoor activity determination in Table 18 show that when the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapyronil + fludioxonil) is 3:1-10:1, it has a synergistic effect on the dark scarab beetle; the mass ratio of the insecticide clothianidin to the fungicide (fluoxathiapyronil + fludioxonil) is (4-8):1, which has a significant synergistic effect on the dark scarab beetle. The mass ratio of clothianidin:fluoxathiapyronil:fludioxonil is (12-40):1:3, which has a synergistic effect on the dark scarab beetle; the mass ratio of clothianidin:fluoxathiapyronil:fludioxonil is (16-32):1:3, which has a significant synergistic effect on the grub; and the mass ratio of clothianidin:fluoxathiapyronil:fludioxonil is 60:1:3, which has an additive effect on the dark scarab beetle.

[0341] Example 7: Experiment on the control effect of different pesticides on major diseases and insect pests in wheat seedling stage

[0342] The test site is Wangyang Village, Youzhao Township, Pingxiang County, Xingtai City, Hebei Province. The soil is clay loam. The test site is flat, easy to irrigate and drain, and has uniform soil fertility. Wheat has been planted in the experimental site for many years, and underground pests such as white grubs, mole crickets, and wireworms have occurred seriously.

[0343] Experimental crops: wheat (Shixin 633).

[0344] Experimental design: Each treatment was repeated 4 times, and each plot was 60m 2 Each plot was randomly arranged in blocks, and protective rows were set up around the plots. Except for the experimental pesticides, the other cultivation and management of the experimental plots were the same as those of the general field during the entire wheat growth period.

[0345] Application time and method: Before sowing wheat, coat the wheat seeds. Mix each agent into a slurry according to the designed dosage, then pour the seeds and the liquid into a plastic bag and stir them thoroughly to mix the seeds and the liquid thoroughly. Spread the mixed wheat seeds to dry before sowing.

[0346] Investigation on the occurrence of underground pests: 30 days after sowing, investigate the damage of white grubs, mole crickets and wireworms. Use the "Z" type sampling method to select 5 points in each plot, investigate 20 plants at each point, for a total of 100 plants, count the number of damaged plants, and calculate the damaged plant rate and prevention effect.

[0347]

[0348] Survey on aphid occurrence: 50 days after seedling emergence, the "Z" type sampling method was used to select 5 points in each plot, 5 wheat plants were selected at each point, and a total of 25 plants were surveyed to investigate the number of aphids on the whole plant and calculate the prevention effect.

[0349]

[0350] Survey on soil-borne diseases of wheat: Investigate the incidence of wheat root rot during the wheat heading stage, and investigate the incidence of wheat stem base rot during the wheat milky stage.

[0351] When investigating wheat root rot, take samples at 5 points in a Z-shaped pattern in each plot, randomly investigate 20 plants at each point, and investigate a total of 100 plants. Carefully dig out the whole plant, keep the root system as intact as possible, wash it in water, investigate the root disease situation against a white background, and grade it according to the percentage of root infection of each plant. The disease is graded according to the following standards: Grade 0: no disease; Grade 1: root disease area accounts for 1% to 5%; Grade 3: root disease area accounts for 6% to 20%; Grade 5: root disease area accounts for 21% to 40%; Grade 7: root disease area accounts for 41% to 60%; Grade 9: root disease area accounts for more than 61%.

[0352] When investigating wheat stem base rot, five diagonal sampling points were used, with 20 plants surveyed at each point, a total of 100 plants, and the disease level and number of white ears of each plant were investigated to calculate the disease index and control effect. The disease was graded according to the following standards: Level 0: no browning symptoms on the whole plant; Level 1: browning at the root; Level 3: browning and rot at the first stem node of the aboveground part; Level 5: browning and rot at the second stem node of the aboveground part; Level 7: lesions exceeded the second stem node, but there were no white ears; Level 9: lesions exceeded the second stem node and there were white ears.

[0353]

[0354] The test results are as follows:

[0355] Table 19 The results of different pesticide treatments on underground pests and aphids in wheat seedling stage

[0356]

[0357]

[0358] Table 20 Results of different pesticide treatments on the control of wheat soil-borne diseases

[0359]

[0360] It can be seen from Tables 19 and 20 that Preparation Example 1: 7.5% clothianidin·fluoxathiapiprolin·fluthiocarbamide seed treatment suspension (6.25%+0.625%+0.625%) and Preparation Example 2: 13.5% clothianidin·fluoxathiapiprolin·fluthiocarbamide seed treatment suspension (12%+0.5%+1%) have good control effects on soil-borne wheat diseases such as wheat root rot, stem base rot, and wheat underground pests, aphids, etc.

[0361] Example 8: Experiment on the control effect of different pesticides on peanut white rot and underground pests

[0362] The test site is Wujiazhuang Village, Xumeng Town, Wulian County, Rizhao City, Shandong Province. The test site has flat geology, fertile soil, and convenient irrigation and drainage. Underground pests and peanut white rot occur frequently in the test site.

[0363] Experimental crop: Peanut (Yuhua No. 6).

[0364] Experimental design: Each treatment was repeated 4 times, and each plot was 30m2 2 , randomized block arrangement.

[0365] Application method: In the experiment, conventional seed dressing and application of pesticides were carried out 1 day before sowing. After dressing, the seeds were dried in a cool, dark and ventilated place for later use.

[0366] Survey on white rot of peanut: Sampling was conducted in August when the white rot of peanut was most serious. A five-point sampling method in a "Z" shape was adopted, with 20 plants surveyed at each point. The disease was graded, the number of diseased plants at each level was recorded, and the incidence rate was calculated based on the recorded results.

[0367] The classification standards for peanut white rot disease are:

[0368] Level 0: no disease;

[0369] Level 1: Lesions only occur at the roots of peanuts, with 10% of cases becoming ill;

[0370] Level 3: Obvious lesions and discolored hyphae appear at the base of the peanut stems, with 30% of the cases becoming ill;

[0371] Level 5: The base of the peanut stem turns black, white mycelium is obvious, and 50% of the peanuts are infected;

[0372] Level 7: The roots of the peanut plant are covered with white hyphae, the whole plant begins to wilt, and 80% of the peanut plant is infected;

[0373] Level 9: The whole peanut plant is completely covered with white mycelium, and the roots turn completely black and die.

[0374] Calculation formula for peanut white rot prevention effect:

[0375]

[0376] Survey on pest population and fruit damage: 5 days before peanut harvest, 4 random sampling points were selected in each plot to investigate pest population. Each sampling point was 1m×1m in area and the digging depth was 30cm. The number of live grubs at each survey point was recorded. The total number of fruits and the number of infested fruits at each sampling point were recorded. The calculation method is as follows:

[0377]

[0378] Yield measurement during harvest period: Yield measurement is carried out during the peanut harvest period and the yield per hectare is converted.

[0379]

[0380] Table 21 The results of different pesticide treatments on the control of peanut white rot

[0381]

[0382] Table 22 Results of different pesticide treatments on peanut underground pest control

[0383]

[0384] Table 23 Effects of different pesticide treatments on peanut yield

[0385]

[0386]

[0387] The results of the field efficacy test showed that the test agent did not cause any phytotoxicity to peanuts within the dosage range of this test. During the entire test investigation period, the test agent showed good control and fruit preservation effects on underground pests of peanuts, and had a significant control effect on white rot of peanuts. The compound composition was safe for peanuts within the dosage range of this test and can be promoted for use.

[0388] Example 9: Experiment on the control effect of different agents on peanut root rot

[0389] Test location: The test was conducted in a peanut field in Dingling Village, Yishan Town, Zoucheng City, Shandong Province. The soil is medium loam, with poor air permeability and above average fertility. Peanuts are planted in the test field all year round, and peanut root rot and grubs are common.

[0390] Experimental crop: peanut (Huayu 35).

[0391] Experimental design: Before sowing peanuts, the seed coating method was used for one-time application. Then the seed coating agent was diluted with water according to the experimental design and poured on the seeds and stirred thoroughly to make the surface of the seeds evenly coated with the drug. After drying, sowing was carried out the next day. The plots in the experimental field were randomly arranged and repeated 4 times.

[0392] On the 60th day after the peanut seedlings emerged, samples were randomly collected at 5 points in each plot, and 20 peanut plants were dug out from each sampling point for disease classification. The number of diseased plants at each level was recorded, and the disease index and control effect were calculated.

[0393] Grading Standard:

[0394] Level 0, no disease;

[0395] Level 1: The roots are slightly discolored, and the discolored roots account for less than 10% of the total roots, and the plants are not wilting;

[0396] Level 3: The roots are obviously brown, and the discolored roots account for 10.1% to 30% of the total roots. The plants begin to wilt.

[0397] Level 5: Discolored roots account for 30.1% to 50% of the total root system, and the plant wilts significantly;

[0398] Level 7: Discolored roots account for 50.1% to 80% of the total root system, and the plant wilts;

[0399] Level 9: The whole plant dies.

[0400] Calculation formula for peanut root rot prevention effect:

[0401]

[0402]

[0403] The test results are as follows:

[0404] Table 24 Effects of different pesticides on the control of peanut root rot

[0405]

[0406] The results of the investigation on the incidence of peanut root rot showed that the treatment with Preparation Example 1: 7.5% clothianidin·fluoxathiapiprolin·fluthiocarbamide seed treatment suspension (6.25%+0.625%+0.625%) with an active ingredient dosage of 80g / 100kg of seeds had the best effect on the prevention and control of peanut root rot, followed by the treatment with Preparation Example 3: 13% clothianidin·fluoxathiapiprolin·fluthiocarbamide seed treatment suspension (12%+0.4%+0.6%) with an active ingredient dosage of 80g / 100kg of seeds (see Table 24).

[0407] In summary, the present invention has verified through experiments that the mixture of fluazifop-butyl, fludioxonil and clothianidin has a better control effect on soil-borne diseases of peanuts and wheat and pests, is safe for target crops, can effectively control the damage of pathogens and pests throughout the growth period of crops, reduces costs, reduces the amount of pesticides used, and has a good guiding significance for field promotion and use.

[0408] Although the present invention has been described in detail above with general descriptions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition containing clothianidin, characterized in that: The pesticide composition comprises a fungicide and an insecticide, wherein the fungicide is fludioxonil and fludioxonil, and the insecticide is clothianidin.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of fludioxonil to fludioxonil in the fungicide is 1:18 to 15:

1.

3. The pesticide composition according to claim 2, characterized in that The mass ratio of fludioxonil to fludioxonil in the fungicide is 1:15 to 15:1, preferably 1:10 to 10:

1.

4. The pesticide composition according to claim 1, characterized in that The mass ratio of the insecticide to the fungicide is 6:1 to 18:

1.

5. The pesticide composition according to claim 4, characterized in that The mass ratio of the insecticide to the fungicide is 6:1 to 16:1, preferably 4:1 to 16:

1.

6. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition being 100 wt%, the total content of the fungicide in the pesticide composition is 0.5% to 50%, preferably 1% to 10%.

7. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition being 100 wt%, the content of the insecticide in the pesticide composition is 1% to 50%, preferably 5% to 35%.

8. The pesticide composition according to claim 1, characterized in that The pesticide composition also includes an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a warning color, a film former, a synergist and a carrier.

9. The pesticide composition according to claim 1, characterized in that The pesticide composition is in the form of any one of a seed treatment suspension, a microcapsule suspension, and a dry seed dressing; Preferably, the pesticide composition is in the form of a seed treatment suspension.

10. Use of the pesticide composition according to any one of claims 1 to 9 for preventing and controlling plant diseases and / or pests, characterized in that: The plants include corn, wheat, rice or peanut; Preferably, the plant is peanut or wheat; the disease is any one of peanut root rot, peanut white rot, peanut stem rot, wheat stem base rot or wheat root rot; The pest is any one of white grubs, cutworms, wireworms or aphids.

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