Application of 5-HTP in improving reproductive performance of sows and growth performance of piglets

Abstract

The invention relates to the technical field of feed additives, in particular to application of 5-HTP to improvement of reproductive performance of sows and growth performance of piglets. The feed containing 5-HTP is used for feeding the sow before delivery, the concentration of serotonin, acetic acid, propionic acid and butyric acid in colon of the sow can be increased, embryo growth can be promoted, the number of live piglets born by the sow and the weight of the live piglets are increased, and the problem that the reproduction and production performance is reduced due to slow development in uterus of the sow is solved.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, and in particular to an application of 5-HTP in improving the reproductive performance of sows and the growth performance of piglets. Background Art

[0002] Sow breeding is the foundation and key of pig breeding, which directly affects the production efficiency of the entire breeding industry. The income of breeding sows mainly comes from the sale of piglets. During the sow's pregnancy, intrauterine growth retardation (IUGR) is one of the common complications. The clinical manifestations are low birth weight of the fetus, growth and development of various tissues and organs are hindered, resulting in low number of live piglets born and high mortality rate of piglets at weaning, causing serious economic losses to the sow breeding industry. Therefore, it is very important to increase the number of live piglets born by sows and the survival rate of piglets.

[0003] In recent years, studies have found that 5-hydroxytryptophan (5-HTP), as an important neurotransmitter precursor, has the function of regulating the nervous, endocrine and immune systems in animals. Although studies have shown that 5-HTP has a certain promoting effect on the growth and reproduction of animals, there are relatively few application studies in the field of sows and piglets. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide an application of 5-HTP in improving the reproductive performance of sows and the growth performance of piglets.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is: use of a feed containing 5-HTP in improving the reproductive performance of sows.

[0006] Another technical solution adopted by the present invention is: use of a feed containing 5-HTP in improving the growth performance of piglets.

[0007] The beneficial effects of the present invention are as follows: the present invention adopts feed containing 5-HTP to feed sows before delivery, which can increase the concentrations of serotonin and acetic acid, propionic acid and butyric acid in the colon of the sows, promote embryo growth, thereby increasing the number of live piglets and the weight of live piglets born by the sows, and solve the problem of reduced reproductive production performance caused by intrauterine growth retardation of the sows. DETAILED DESCRIPTION

[0008] In order to explain the technical content, achieved objectives and effects of the present invention in detail, the following is an explanation in conjunction with the implementation modes.

[0009] The invention discloses an application of a feed containing 5-HTP in improving the reproductive performance of sows, that is, an application of 5-HTP in preparing a feed for improving the reproductive performance of sows.

[0010] From the above description, it can be seen that the beneficial effects of the present invention are: 5-hydroxytryptophan is an amino acid substance that can be converted into serotonin in the body. Since serotonin cannot pass through the blood-brain barrier to enter the brain, 5-HTP must first pass through the blood-brain barrier and then be converted into serotonin in the brain. In view of the problem that sows are prone to intrauterine growth retardation, which leads to reduced reproductive performance, 5-HTP is used to feed sows before delivery, which improves the concentration of serotonin and short-chain fatty acids (acetic acid, propionic acid and butyric acid) in the colon of sows, thereby improving placental development and function, promoting embryonic growth, and thus increasing the number of live piglets born by sows and the weight of live piglets.

[0011] Furthermore, a use of 5-HTP in the preparation of a feed additive for improving the reproductive performance of sows.

[0012] Furthermore, the feed is used to increase the number of piglets born alive and / or the weight of piglets born alive.

[0013] Furthermore, 5-HTP can be added directly to the diet, or made into feed and added indirectly to the diet.

[0014] From the above description, we can know that conventional feed additives such as tryptophan can be used to increase serotonin in sows, but 95% of the metabolic pathway of tryptophan in animals goes through the kynurenic acid pathway; 2-3% goes through the microbial metabolic pathway; only 1-2% is converted into serotonin through the 5-hydroxytryptophan pathway, and tryptophan must first be converted into 5-hydroxytryptophan by tryptophan hydroxylase, which is a rate-limiting enzyme with a slow conversion rate. Therefore, increasing serotonin in the body by ingesting tryptophan is very limited, the conversion rate is slow, and the required dosage is very high, while directly using 5-HTP can quickly increase serotonin in the body.

[0015] Furthermore, the amount of 5-HTP added to the diet is 100~1000 mg / kg.

[0016] Preferably, the amount of 5-HTP added to the diet is 500-1000 mg / kg.

[0017] Furthermore, sows were fed a diet supplemented with 5-HTP from day 30 of gestation until farrowing.

[0018] From the above description, we can know that after the sow is mated, the fertilized egg begins to implant between the 9th and 13th days, and the embryo implantation is basically completed around the 30th day. At this time, the embryo is relatively stable, and the nutritional needs of the sow begin to gradually shift to fetal development and self-maintenance. This is a critical period for adjusting nutritional supply.

[0019] Another technical solution adopted by the present invention is: application of a feed containing 5-HTP in improving the growth performance of piglets, that is, application of 5-HTP in preparing a feed for improving the growth performance of piglets.

[0020] Furthermore, feed additives are used to increase the body weight of piglets.

[0021] From the above description, it can be seen that 5-HTP can increase the feed intake and milk secretion of sows and improve the growth weight of piglets after delivery.

[0022] Furthermore, the piglets are fed with milk produced by sows until weaning, and the sows are fed with a diet supplemented with 5-HTP.

[0023] Furthermore, the amount of 5-HTP added to the diet is 100~1000 mg / kg.

[0024] Preferably, the amount of 5-HTP added to the diet is 200-500 mg / kg.

[0025] The ingredients of the diets in the following examples are shown in Table 1, and the nutritional levels of the diets are shown in Table 2; the experiment was conducted in autumn (October), and 140 healthy multiparous large white sows on the 30th day of pregnancy were selected as the test animals, and were evenly distributed into pig houses with the same conditions, and all sows had free access to water. On the 107th day of pregnancy, all sows were cleaned and disinfected and transferred to the delivery room and raised in the delivery pen. The piglets were selected from the piglets born by the above sows.

[0026] Table 1

[0027] Table 2

[0028] Note: Premix 1: Each kg of basic sow feed (daily diet) provides the following nutrients: zinc, 60 mg; iron, 95 mg; copper, 10 mg; iodine, 0.35 mg; selenium, 0.3 mg; manganese, 80 mg; vitamin A, 12,000 IU; vitamin D3, 2,750 IU; vitamin E, 30 IU; vitamin K3, 2 mg; vitamin B12, 12 μg; vitamin B2, 6 mg; niacin, 40 mg; pantothenic acid, 12 mg; vitamin B6, 3 mg; biotin, 0.2 mg.

[0029] Embodiment 1 of the present invention is: an application of a feed containing 5-HTP in improving the reproductive performance of sows, and the specific steps are: 100 mg / kg 5-HTP is directly added to the diet, and the sows are fed with the diet supplemented with 5-HTP from the 30th day of gestation to production, so as to increase the number of live piglets born by the sows and the weight of live piglets.

[0030] Embodiment 2 of the present invention is: The only difference between Example 2 and Example 1 is that the added amount of 5-HTP is 200 mg / kg.

[0031] Embodiment 3 of the present invention is: The only difference between Example 3 and Example 1 is that the added amount of 5-HTP is 500 mg / kg.

[0032] Embodiment 4 of the present invention is: The only difference between Example 4 and Example 1 is that the added amount of 5-HTP is 1000 mg / kg.

[0033] Comparative Example 1 of the present invention is: The difference between Comparative Example 1 and Example 1 is that 5-HTP was not added to the diet, but 1000 mg / kg tryptophan was added.

[0034] Comparative Example 2 of the present invention is: The difference between Comparative Example 2 and Example 1 is that 5-HTP was not added to the diet, but 2000 mg / kg tryptophan was added.

[0035] Comparative Example 3 of the present invention is: The only difference between Comparative Example 3 and Example 1 is that 5-HTP was not added to the diet, but 3000 mg / kg tryptophan was added.

[0036] Comparative Example 4 of the present invention is: The only difference between Comparative Example 4 and Example 1 is that 5-HTP is not added to the diet.

[0037] The sows of Examples 1 to 4 and Comparative Examples 1 to 4 were tested, and the test items were as follows: 1. Five sows were randomly selected from each treatment group on the 30th, 60th and 107th day of gestation, and blood was collected to test serotonin, plasma immune indicators (IgM, IgA, IgG), antioxidant levels (superoxide dismutase (SOD), malondialdehyde (MDA)), blood sugar, total cholesterol and triglyceride indicators. The above recorded data results were processed and the data were analyzed by one-way analysis of variance (ANOVA) using SPSS statistical software. P<0.05 was considered to be significantly different, and P<0.01 was considered to be extremely significantly different. The data processing results are shown in Tables 3 and 4.

[0038] Table 3

[0039] Table 4

[0040] From the results in Tables 3 and 4, we can see that compared with the control group (Comparative Example 4), the 5-HTP group significantly increased the plasma serotonin level on the 60th and 107th days (P<0.05), significantly increased the immunoglobulin IgG level, significantly increased the plasma SOD level, and reduced the plasma MDA level (P<0.05). This shows that the reproductive performance of multiparous sows can be improved by regulating the plasma serotonin level of pregnant sows, improving antioxidant capacity and immune function, etc. Compared with the control group (Comparative Example 4), the tryptophan feeding group (Comparative Examples 1 to 3) had no significant difference in plasma serotonin, plasma SOD level and plasma MDA (P>0.05). Only the 3000 mg / kg tryptophan group significantly increased the immunoglobulin IgG level on the 107th day, which may have enhanced the immunity of sows through other metabolic pathways.

[0041] 2. Five sows were randomly selected from each treatment group on the 30th, 60th and 107th days of gestation, and feces were collected to detect the levels of short-chain fatty acids (acetic acid, propionic acid, isobutyric acid, butyric acid and valeric acid). The data recorded above were processed and the data were analyzed by one-way analysis of variance (ANOVA) using SPSS statistical software. P<0.05 was considered to be significantly different, and P<0.01 was considered to be extremely significantly different. The data processing results are shown in Table 5.

[0042] Table 5

[0043] From the results in Table 5, it can be seen that the levels of acetic acid, propionic acid, butyric acid and total short-chain fatty acids in the feces of sows on the 60th and 107th days were significantly increased in the 5-HTP group (Example 1) compared with the control group (Comparative Example 4) (P<0.05). Short-chain fatty acids can not only promote the proliferation of probiotics, improve the intestinal function of sows, increase the feed intake and milk secretion of sows, improve the growth of sow placenta and the growth of piglets after delivery, thereby improving the reproductive performance of sows and the growth performance of piglets. Compared with the control group (Comparative Example 4), there were no significant differences in the indicators of short-chain fatty acids in the tryptophan feeding group (Comparative Examples 1 to 3) (P>0.05).

[0044] 3. The number and weight of piglets born alive by sows were counted. The statistical results are shown in Table 6.

[0045] Table 6

[0046] From the results in Table 6, it can be seen that the feeding of 5-HTP groups (Examples 1 to 4) had no significant effect on the number of piglets born by sows compared with the control group (Comparative Example 4) (P>0.05), and significantly increased the number of live piglets and live piglet weight (P<0.05). The results of feeding 5-HTP at a dose of 500 mg / kg (Example 3) were relatively the best, with an average increase of 2.5 live piglets and an average increase of 0.37 kg live piglet weight. There was no significant difference in the results of the feeding of tryptophan groups (Comparative Examples 1 to 3) compared with the control group (Comparative Example 4) (P>0.05).

[0047] Embodiment 5 of the present invention is: an application of a feed containing 5-HTP in improving the growth performance of piglets. The specific steps are: 100 mg / kg 5-HTP is directly added to the diet, sows are fed with the diet supplemented with 5-HTP, and then the piglets are fed with milk produced by the sows until weaning to increase the weight of the piglets.

[0048] Embodiment 6 of the present invention is: The only difference between Example 6 and Example 5 is that the added amount of 5-HTP is 200 mg / kg.

[0049] Embodiment 7 of the present invention is: The only difference between Example 7 and Example 5 is that the added amount of 5-HTP is 500 mg / kg.

[0050] Embodiment 8 of the present invention is: The only difference between Example 8 and Example 5 is that the added amount of 5-HTP is 1000 mg / kg.

[0051] Comparative Example 5 of the present invention is: The only difference between Comparative Example 5 and Example 5 is that 5-HTP was not added to the diet, but 1000 mg / kg tryptophan was added.

[0052] Comparative Example 6 of the present invention is: The only difference between Comparative Example 6 and Example 5 is that 5-HTP was not added to the diet, but 2000 mg / kg tryptophan was added.

[0053] Comparative Example 7 of the present invention is: The only difference between Comparative Example 7 and Example 5 is that 5-HTP was not added to the diet, but 3000 mg / kg tryptophan was added.

[0054] Comparative Example 8 of the present invention is: The only difference between Comparative Example 8 and Example 5 is that 5-HTP was not added to the diet.

[0055] The piglet survival rates and piglet weaning weights of Examples 5 to 8 and Comparative Examples 5 to 8 were statistically analyzed, and the statistical results are shown in Table 7.

[0056] Table 7

[0057] From the results in Table 7, it can be seen that the 5-HTP feeding group (Examples 5 to 8) significantly increased the piglet survival rate and weaning weight compared with the control group (Comparative Example 8) (P<0.05), and the result of feeding 5-HTP at a dose of 200 mg / kg (Example 6) was relatively the best, with an average increase of 12.93% in piglet survival rate and an average increase of 1.56 kg in piglet weaning weight. There was no significant difference in the results between the tryptophan feeding group (Comparative Examples 5 to 7) and the control group (Comparative Example 8) (P>0.05).

[0058] In summary, the application of 5-HTP provided by the present invention in improving the reproductive performance of sows and the growth performance of piglets, using 5-HTP to feed sows before delivery can increase the number of live piglets born by sows and the weight of live piglets; feeding piglets with sow milk fed with 5-HTP can increase the weight of piglets.

[0059] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent transformations made using the specification of the present invention, or directly or indirectly applied in related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. Application of a feed containing 5-HTP in improving the reproductive performance of sows.

2. The use according to claim 1, characterized in that: The feed is used for increasing the number of live piglets born alive and / or the weight of live piglets.

3. The use according to claim 1, characterized in that: The 5-HTP can be added directly to the diet, or made into feed and added to the diet indirectly.

4. The use according to claim 3, characterized in that: The amount of 5-HTP added to the diet is 100-1000 mg / kg.

5. The use according to claim 4, characterized in that: The amount of 5-HTP added to the diet is 500-1000 mg / kg.

6. The use according to claim 3, characterized in that: The sows were fed a diet supplemented with 5-HTP from day 30 of gestation until farrowing.

7. Use of a feed comprising 5-HTP in improving the growth performance of piglets.

8. The use according to claim 7, characterized in that: The feed additive is used for increasing the body weight of piglets.

9. The use according to claim 8, characterized in that: The piglets were fed until weaning using milk produced by sows fed a diet supplemented with 5-HTP.

10. The use according to claim 8, characterized in that: The amount of 5-HTP added to the diet is 100-1000 mg / kg.

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