Microecological preparation for reconstructing intestinal microecology as well as preparation method and application thereof

Abstract

The invention relates to a microecological preparation for reconstructing intestinal microecology. The microecological preparation is prepared from the following raw materials in parts by weight: 8-12 parts of probiotics, 3-5 parts of prebiotics and 2-4 parts of metabiotics, the probiotics are selected from one of lactic acid bacteria and bifidobacteria, and have the functions of regulating intestinal flora and enhancing immunity. Specific probiotics, prebiotics and metabiotics are selected and have a synergistic effect, the survival rate of the probiotics is increased, colonization and proliferation of the probiotics are promoted, intestinal flora is more effectively adjusted, accurate regulation is achieved, a personalized formula is designed according to the characteristics of the intestinal flora of different people, accurate regulation is achieved, and safety and convenience are achieved.

Description

Technical Field

[0001] The present invention belongs to the field of biotechnology, and specifically relates to a microecological preparation for rebuilding intestinal microecology, and a preparation method and application thereof, and in particular to a microecological preparation containing specific probiotics, prebiotics and postbiotics, which is used to regulate the balance of intestinal flora and improve intestinal health. Background Art

[0002] Intestinal microecology is closely related to human health, and intestinal flora imbalance is related to a variety of diseases. At present, the methods for regulating intestinal flora mainly include: probiotic preparations: directly supplementing live bacteria, but there are problems such as low survival rate and difficulty in colonization; prebiotic preparations: promoting the growth of beneficial bacteria, but lack of specificity and limited effect; fecal microbiota transplantation: significant effect, but there are safety and ethical issues. Based on this, the present invention further improves and processes them. Summary of the invention

[0003] In view of the defects of the prior art, the purpose of the present invention is to provide a microecological preparation for reconstructing intestinal microecology and a preparation method and application thereof, so as to solve the problems raised in the above background technology.

[0004] The present invention solves the technical problem by adopting the following technical solution:

[0005] The present invention provides a microecological preparation for rebuilding intestinal microecology, the microecological preparation comprising the following raw materials in parts by weight: 8 to 12 parts of probiotics, 3 to 5 parts of prebiotics, and 2 to 4 parts of postbiotics;

[0006] The microecological preparation further comprises 5 to 8 parts of a modifier doped with montmorillonite and 3 to 4 parts of an auxiliary adjusting agent modified by nano-silicon dioxide.

[0007] Preferably, the probiotics are selected from lactic acid bacteria and bifidobacteria, and have the functions of regulating intestinal flora and enhancing immunity;

[0008] The prebiotic is selected from one of oligofructose and oligogalactose to promote the growth of probiotics;

[0009] Postbiotics are selected from one of the groups such as short-chain fatty acids and bacteriocin, and have antibacterial and anti-inflammatory effects.

[0010] Preferably, the preparation method of the montmorillonite-doped modifier is:

[0011] S01: adding montmorillonite to a sodium alginate solution of 3-5 times the total amount of montmorillonite, and then adding a phosphate buffer solution of 5-10% of the total amount of montmorillonite, stirring sufficiently to obtain a montmorillonite agent;

[0012] S02: adding 2-3 parts of hydroxyapatite and 1-3 parts of nano-silica sol to 2-4 parts of chitosan solution, and stirring thoroughly to obtain hydroxyapatite liquid;

[0013] S03: Mix the montmorillonite agent and the hydroxyapatite liquid in a weight ratio of 5:3 and perform ball milling at a ball milling speed of 1000-1500 r / min for 1 hour. After the ball milling is completed, filter and dry to obtain a modifier doped with montmorillonite.

[0014] Preferably, the mass fraction of the sodium alginate solution is 2-5%; the mass fraction of the chitosan solution is 3-6%.

[0015] Preferably, the pH value of the phosphate buffer solution is 5.0.

[0016] Preferably, the preparation method of the nano-silicon dioxide-modified auxiliary agent is:

[0017] S101: adding nano-silicon dioxide into sulfuric acid solution in a weight ratio of 2:7 and stirring thoroughly, then washing with water and drying;

[0018] Preheating the dried nano-silicon dioxide to 65-70° C. for 1 hour to obtain preheated nano-silicon dioxide;

[0019] S102: immersing the preheated nano-silicon dioxide into the modified liquid for ultrasonic treatment, filtering and drying after the immersion is completed, to obtain an auxiliary agent modified by the nano-silicon dioxide;

[0020] The modified liquid comprises 2-5 parts by weight of sodium stearate, 1-3 parts of carboxymethyl cellulose, 5-8 parts of sodium dodecylbenzene sulfonate solution and 2-3 parts of citric acid.

[0021] Preferably, the mass fraction of the sulfuric acid solution is 2-5%; the mass fraction of the sodium dodecylbenzenesulfonate solution is 4-8%.

[0022] Preferably, the ultrasonic power of the immersion ultrasonic treatment is 400-500W, and the ultrasonic treatment is performed for 1 hour.

[0023] The present invention also provides a method for preparing a microecological preparation for reconstructing intestinal microecology, comprising the following steps:

[0024] The probiotics, prebiotics, postbiotics, montmorillonite modifiers and nano-silica-modified adjusting agents are fully blended to obtain a microecological preparation for rebuilding the intestinal microecology.

[0025] The present invention also provides an application of a microecological preparation for rebuilding intestinal microecology in preventing and assisting in treating intestinal flora imbalance.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The present invention has synergistic effects: specific probiotics, prebiotics and postbiotics are carefully selected, and the three work synergistically to improve the survival rate of probiotics, promote their colonization and proliferation, and more effectively regulate the intestinal flora. Precise regulation: personalized formulas are designed according to the characteristics of intestinal flora of different populations to achieve precise regulation. Safe and convenient: advanced preparation technology is adopted to ensure product safety and stability, and it is easy to use. At the same time, the modifier doped with montmorillonite and the auxiliary adjustment agent modified by nano-silica are added to work together to enhance the effect, optimize the effects of probiotics, prebiotics and postbiotics on intestinal flora, thereby improving the therapeutic effect of microecological preparations and optimizing product performance effects. DETAILED DESCRIPTION

[0028] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] A microecological preparation for rebuilding intestinal microecology in this embodiment, the microecological preparation comprises the following raw materials in parts by weight: 8 to 12 parts of probiotics, 3 to 5 parts of prebiotics, and 2 to 4 parts of postbiotics;

[0030] The microecological preparation further comprises 5 to 8 parts of a modifier doped with montmorillonite and 3 to 4 parts of an auxiliary adjusting agent modified by nano-silicon dioxide.

[0031] The probiotics in this embodiment are selected from lactic acid bacteria and bifidobacteria, and have the functions of regulating intestinal flora and enhancing immunity;

[0032] The prebiotic is selected from one of oligofructose and oligogalactose to promote the growth of probiotics;

[0033] Postbiotics are selected from one of the groups such as short-chain fatty acids and bacteriocin, and have antibacterial and anti-inflammatory effects.

[0034] The preparation method of the modifier doped with montmorillonite in this embodiment is:

[0035] S01: adding montmorillonite to a sodium alginate solution of 3-5 times the total amount of montmorillonite, and then adding a phosphate buffer solution of 5-10% of the total amount of montmorillonite, stirring sufficiently to obtain a montmorillonite agent;

[0036] S02: adding 2-3 parts of hydroxyapatite and 1-3 parts of nano-silica sol to 2-4 parts of chitosan solution, and stirring thoroughly to obtain hydroxyapatite liquid;

[0037] S03: Mix the montmorillonite agent and the hydroxyapatite liquid in a weight ratio of 5:3 and perform ball milling at a ball milling speed of 1000-1500 r / min for 1 hour. After the ball milling is completed, filter and dry to obtain a modifier doped with montmorillonite.

[0038] The mass fraction of the sodium alginate solution in this embodiment is 2-5%; the mass fraction of the chitosan solution is 3-6%.

[0039] The pH value of the phosphate buffer solution in this example is 5.0.

[0040] The preparation method of the nano-silicon dioxide-modified auxiliary agent in this embodiment is as follows:

[0041] S101: adding nano-silicon dioxide into sulfuric acid solution in a weight ratio of 2:7 and stirring thoroughly, then washing with water and drying;

[0042] Preheating the dried nano-silicon dioxide to 65-70° C. for 1 hour to obtain preheated nano-silicon dioxide;

[0043] S102: immersing the preheated nano-silicon dioxide into the modified liquid for ultrasonic treatment, filtering and drying after the immersion is completed, to obtain an auxiliary agent modified by the nano-silicon dioxide;

[0044] The modified liquid comprises 2-5 parts by weight of sodium stearate, 1-3 parts of carboxymethyl cellulose, 5-8 parts of sodium dodecylbenzene sulfonate solution and 2-3 parts of citric acid.

[0045] The mass fraction of the sulfuric acid solution in this embodiment is 2-5%; the mass fraction of the sodium dodecylbenzene sulfonate solution is 4-8%.

[0046] The ultrasonic power of the immersion ultrasonic treatment in this embodiment is 400-500W, and the ultrasonic treatment is performed for 1 hour.

[0047] A method for preparing a microecological preparation for reconstructing intestinal microecology in this embodiment comprises the following steps:

[0048] The probiotics, prebiotics, postbiotics, montmorillonite modifiers and nano-silica-modified adjusting agents are fully blended to obtain a microecological preparation for rebuilding the intestinal microecology.

[0049] The present invention also provides an application of a microecological preparation for rebuilding intestinal microecology in preventing and assisting in treating intestinal flora imbalance.

[0050] Example 1.

[0051] A microecological preparation for rebuilding intestinal microecology in this embodiment, the microecological preparation comprises the following raw materials in parts by weight: 8 parts of probiotics, 3 parts of prebiotics, and 1 part of postbiotics;

[0052] The microecological preparation further comprises 5 parts of a modifier doped with montmorillonite and 3 parts of an auxiliary adjusting agent modified by nano-silicon dioxide.

[0053] The probiotics in this embodiment are selected from lactic acid bacteria, which have the functions of regulating intestinal flora and enhancing immunity;

[0054] Prebiotics are selected from oligofructose to promote the growth of probiotics;

[0055] Postbiotics are selected from, for example, short-chain fatty acids, which have antibacterial and anti-inflammatory effects.

[0056] The preparation method of the modifier doped with montmorillonite in this embodiment is:

[0057] S01: adding montmorillonite to a sodium alginate solution of 3 times the total amount of montmorillonite, and then adding a phosphate buffer solution of 5% of the total amount of montmorillonite, stirring sufficiently to obtain a montmorillonite agent;

[0058] S02: Add 2 parts of hydroxyapatite and 1 part of nano-silica sol to 2 parts of chitosan solution, and stir thoroughly to obtain hydroxyapatite liquid;

[0059] S03: The montmorillonite agent and the hydroxyapatite liquid are mixed and ball-milled in a weight ratio of 5:3, with a ball-milling speed of 1000 r / min for 1 hour. After the ball-milling is completed, the mixture is filtered and dried to obtain a modifier doped with montmorillonite.

[0060] The mass fraction of the sodium alginate solution in this embodiment is 2%; the mass fraction of the chitosan solution is 3%.

[0061] The pH value of the phosphate buffer solution in this example is 5.0.

[0062] The preparation method of the nano-silicon dioxide-modified auxiliary agent in this embodiment is as follows:

[0063] S101: adding nano-silicon dioxide into sulfuric acid solution in a weight ratio of 2:7 and stirring thoroughly, then washing with water and drying;

[0064] Preheating the dried nano-silica to 65° C. for 1 hour to obtain preheated nano-silica;

[0065] S102: immersing the preheated nano-silicon dioxide into the modified liquid for ultrasonic treatment, filtering and drying after the immersion is completed, to obtain an auxiliary agent modified by the nano-silicon dioxide;

[0066] The modified liquid is 2 parts by weight of sodium stearate, 1 part of carboxymethyl cellulose, 5 parts of sodium dodecylbenzene sulfonate solution and 2 parts of citric acid.

[0067] The mass fraction of the sulfuric acid solution in this embodiment is 2%; the mass fraction of the sodium dodecylbenzene sulfonate solution is 4%.

[0068] The ultrasonic power of the immersion ultrasonic treatment in this embodiment is 400 W, and the ultrasonic treatment is performed for 1 hour.

[0069] A method for preparing a microecological preparation for reconstructing intestinal microecology in this embodiment comprises the following steps:

[0070] The probiotics, prebiotics, postbiotics, montmorillonite modifiers and nano-silica-modified adjusting agents are fully blended to obtain a microecological preparation for rebuilding the intestinal microecology.

[0071] The present invention also provides an application of a microecological preparation for rebuilding intestinal microecology in preventing and assisting in treating intestinal flora imbalance.

[0072] Example 2.

[0073] A microecological preparation for rebuilding intestinal microecology in this embodiment, the microecological preparation comprises the following raw materials in parts by weight: 12 parts of probiotics, 5 parts of prebiotics, and 4 parts of postbiotics;

[0074] The microecological preparation further comprises 8 parts of a modifier doped with montmorillonite and 4 parts of an auxiliary adjusting agent modified by nano-silicon dioxide.

[0075] The probiotics in this embodiment are selected from Bifidobacterium, which has the function of regulating intestinal flora and enhancing immunity;

[0076] Prebiotics are selected from galacto-oligosaccharides to promote the growth of probiotics;

[0077] Postbiotics are selected from bacteriocins, which have antibacterial and anti-inflammatory effects.

[0078] The preparation method of the modifier doped with montmorillonite in this embodiment is:

[0079] S01: adding montmorillonite to a sodium alginate solution of 5 times the total amount of montmorillonite, and then adding a phosphate buffer solution of 10% of the total amount of montmorillonite, stirring sufficiently to obtain a montmorillonite agent;

[0080] S02: Add 3 parts of hydroxyapatite and 3 parts of nano-silica sol to 4 parts of chitosan solution, and stir thoroughly to obtain hydroxyapatite liquid;

[0081] S03: The montmorillonite agent and the hydroxyapatite liquid are mixed and ball-milled in a weight ratio of 5:3, with a ball-milling speed of 1500 r / min for 1 h. After the ball-milling is completed, the mixture is filtered and dried to obtain a modifier doped with montmorillonite.

[0082] The mass fraction of the sodium alginate solution in this embodiment is 5%; the mass fraction of the chitosan solution is 6%.

[0083] The pH value of the phosphate buffer solution in this example is 5.0.

[0084] The preparation method of the nano-silicon dioxide-modified auxiliary agent in this embodiment is as follows:

[0085] S101: adding nano-silicon dioxide into sulfuric acid solution in a weight ratio of 2:7 and stirring thoroughly, then washing with water and drying;

[0086] Preheating the dried nano-silicon dioxide to 70° C. for 1 hour to obtain preheated nano-silicon dioxide;

[0087] S102: immersing the preheated nano-silicon dioxide into the modified liquid for ultrasonic treatment, filtering and drying after the immersion is completed, to obtain an auxiliary agent modified by the nano-silicon dioxide;

[0088] The modified liquid is 5 parts by weight of sodium stearate, 3 parts of carboxymethyl cellulose, 8 parts of sodium dodecylbenzene sulfonate solution and 3 parts of citric acid.

[0089] The mass fraction of the sulfuric acid solution in this embodiment is 5%; the mass fraction of the sodium dodecylbenzene sulfonate solution is 8%.

[0090] The ultrasonic power of the immersion ultrasonic treatment in this embodiment is 500W, and the ultrasonic treatment is performed for 1 hour.

[0091] A method for preparing a microecological preparation for reconstructing intestinal microecology in this embodiment comprises the following steps:

[0092] The probiotics, prebiotics, postbiotics, montmorillonite modifiers and nano-silica-modified adjusting agents are fully blended to obtain a microecological preparation for rebuilding the intestinal microecology.

[0093] The present invention also provides an application of a microecological preparation for rebuilding intestinal microecology in preventing and assisting in treating intestinal flora imbalance.

[0094] Example 3.

[0095] A microecological preparation for rebuilding intestinal microecology in this embodiment, the microecological preparation comprises the following raw materials in parts by weight: 10 parts of probiotics, 4 parts of prebiotics, and 3 parts of postbiotics;

[0096] The microecological preparation further comprises 6.5 parts of a modifier doped with montmorillonite and 3.5 parts of an auxiliary adjusting agent modified by nano-silicon dioxide.

[0097] The probiotics in this embodiment are selected from lactic acid bacteria, which have the functions of regulating intestinal flora and enhancing immunity;

[0098] Prebiotics are selected from oligofructose to promote the growth of probiotics;

[0099] Postbiotics are selected from, for example, short-chain fatty acids, which have antibacterial and anti-inflammatory effects.

[0100] The preparation method of the modifier doped with montmorillonite in this embodiment is:

[0101] S01: adding montmorillonite to a sodium alginate solution of 4 times the total amount of montmorillonite, and then adding a phosphate buffer solution of 7.5% of the total amount of montmorillonite, stirring thoroughly, to obtain a montmorillonite agent;

[0102] S02: Add 2.5 parts of hydroxyapatite and 2 parts of nano-silica sol to 3 parts of chitosan solution, and stir thoroughly to obtain hydroxyapatite liquid;

[0103] S03: The montmorillonite agent and the hydroxyapatite liquid are mixed and ball-milled in a weight ratio of 5:3, with a ball-milling speed of 1250 r / min for 1 h. After the ball-milling is completed, the mixture is filtered and dried to obtain a modifier doped with montmorillonite.

[0104] The mass fraction of the sodium alginate solution in this embodiment is 3.5, and the mass fraction of the chitosan solution is 4.5%.

[0105] The pH value of the phosphate buffer solution in this example is 5.0.

[0106] The preparation method of the nano-silicon dioxide-modified auxiliary agent in this embodiment is as follows:

[0107] S101: adding nano-silicon dioxide into sulfuric acid solution in a weight ratio of 2:7 and stirring thoroughly, then washing with water and drying;

[0108] Preheating the dried nano-silicon dioxide to 67.5° C. for 1 hour to obtain preheated nano-silicon dioxide;

[0109] S102: immersing the preheated nano-silicon dioxide into the modified liquid for ultrasonic treatment, filtering and drying after the immersion is completed, to obtain an auxiliary agent modified by the nano-silicon dioxide;

[0110] The modified liquid is 3.5 parts by weight of sodium stearate, 2 parts of carboxymethyl cellulose, 6.5 parts of sodium dodecylbenzene sulfonate solution and 2.5 parts of citric acid.

[0111] The mass fraction of the sulfuric acid solution in this embodiment is 3.5%; the mass fraction of the sodium dodecylbenzene sulfonate solution is 6%.

[0112] The ultrasonic power of the immersion ultrasonic treatment in this embodiment is 450 W, and the ultrasonic treatment is performed for 1 hour.

[0113] A method for preparing a microecological preparation for reconstructing intestinal microecology in this embodiment comprises the following steps:

[0114] The probiotics, prebiotics, postbiotics, montmorillonite modifiers and nano-silica-modified adjusting agents are fully blended to obtain a microecological preparation for rebuilding the intestinal microecology.

[0115] The present invention also provides an application of a microecological preparation for rebuilding intestinal microecology in preventing and assisting in treating intestinal flora imbalance.

[0116] Example 4.

[0117] A microecological preparation for rebuilding intestinal microecology in this embodiment, the microecological preparation comprises the following raw materials in parts by weight: 9 parts of probiotics, 4 parts of prebiotics, and 3 parts of postbiotics;

[0118] The microecological preparation further comprises 6 parts of a modifier doped with montmorillonite and 3 parts of an auxiliary adjusting agent modified by nano-silicon dioxide.

[0119] The probiotics in this embodiment are selected from lactic acid bacteria, which have the functions of regulating intestinal flora and enhancing immunity;

[0120] Prebiotics are selected from oligofructose, which promote the growth of probiotics;

[0121] Postbiotics are selected from, for example, short-chain fatty acids, which have antibacterial and anti-inflammatory effects.

[0122] The preparation method of the modifier doped with montmorillonite in this embodiment is:

[0123] S01: adding montmorillonite to a sodium alginate solution of 4 times the total amount of montmorillonite, and then adding a phosphate buffer solution of 6% of the total amount of montmorillonite, stirring thoroughly, to obtain a montmorillonite agent;

[0124] S02: Add 2 parts of hydroxyapatite and 2 parts of nano-silica sol to 3 parts of chitosan solution, and stir thoroughly to obtain hydroxyapatite liquid;

[0125] S03: The montmorillonite agent and the hydroxyapatite liquid are mixed and ball-milled in a weight ratio of 5:3, with a ball-milling speed of 1200 r / min for 1 h. After the ball-milling is completed, the mixture is filtered and dried to obtain a modifier doped with montmorillonite.

[0126] The mass fraction of the sodium alginate solution in this embodiment is 3%; the mass fraction of the chitosan solution is 4%.

[0127] The pH value of the phosphate buffer solution in this example is 5.0.

[0128] The preparation method of the nano-silicon dioxide-modified auxiliary agent in this embodiment is as follows:

[0129] S101: adding nano-silicon dioxide into sulfuric acid solution in a weight ratio of 2:7 and stirring thoroughly, then washing with water and drying;

[0130] Preheating the dried nano-silica to 66° C. for 1 hour to obtain preheated nano-silica;

[0131] S102: immersing the preheated nano-silicon dioxide into the modified liquid for ultrasonic treatment, filtering and drying after the immersion is completed, to obtain an auxiliary agent modified by the nano-silicon dioxide;

[0132] The modified liquid is 3 parts by weight of sodium stearate, 2 parts of carboxymethyl cellulose, 6 parts of sodium dodecylbenzene sulfonate solution and 3 parts of citric acid.

[0133] The mass fraction of the sulfuric acid solution in this embodiment is 3%; the mass fraction of the sodium dodecylbenzene sulfonate solution is 5%.

[0134] The ultrasonic power of the immersion ultrasonic treatment in this embodiment is 420W, and the ultrasonic treatment is performed for 1 hour.

[0135] A method for preparing a microecological preparation for reconstructing intestinal microecology in this embodiment comprises the following steps:

[0136] The probiotics, prebiotics, postbiotics, montmorillonite modifiers and nano-silica-modified adjusting agents are fully blended to obtain a microecological preparation for rebuilding the intestinal microecology.

[0137] The present invention also provides an application of a microecological preparation for rebuilding intestinal microecology in preventing and assisting in treating intestinal flora imbalance.

[0138] Example 5.

[0139] A microecological preparation for rebuilding intestinal microecology in this embodiment, the microecological preparation comprises the following raw materials in parts by weight: 11 parts of probiotics, 4 parts of prebiotics, and 3 parts of postbiotics;

[0140] The microecological preparation further comprises 5 parts of a modifier doped with montmorillonite and 4 parts of an auxiliary adjusting agent modified by nano-silicon dioxide.

[0141] The probiotics in this embodiment are selected from lactic acid bacteria, which have the functions of regulating intestinal flora and enhancing immunity;

[0142] Prebiotics are selected from galacto-oligosaccharides to promote the growth of probiotics;

[0143] Postbiotics are selected from bacteriocins, which have antibacterial and anti-inflammatory effects.

[0144] The preparation method of the modifier doped with montmorillonite in this embodiment is:

[0145] S01: adding montmorillonite to a sodium alginate solution of 5 times the total amount of montmorillonite, and then adding a phosphate buffer solution of 8% of the total amount of montmorillonite, stirring sufficiently to obtain a montmorillonite agent;

[0146] S02: Add 3 parts of hydroxyapatite and 2 parts of nano-silica sol to 3 parts of chitosan solution, and stir thoroughly to obtain hydroxyapatite liquid;

[0147] S03: The montmorillonite agent and the hydroxyapatite liquid are mixed and ball-milled in a weight ratio of 5:3, with a ball-milling speed of 1450 r / min for 1 h. After the ball-milling is completed, the mixture is filtered and dried to obtain a modifier doped with montmorillonite.

[0148] The mass fraction of the sodium alginate solution in this embodiment is 4%; the mass fraction of the chitosan solution is 5%.

[0149] The pH value of the phosphate buffer solution in this example is 5.0.

[0150] The preparation method of the nano-silicon dioxide-modified auxiliary agent in this embodiment is as follows:

[0151] S101: adding nano-silicon dioxide into sulfuric acid solution in a weight ratio of 2:7 and stirring thoroughly, then washing with water and drying;

[0152] Preheating the dried nano-silica to 68° C. for 1 hour to obtain preheated nano-silica;

[0153] S102: immersing the preheated nano-silicon dioxide into the modified liquid for ultrasonic treatment, filtering and drying after the immersion is completed, to obtain an auxiliary agent modified by the nano-silicon dioxide;

[0154] The modified liquid is 4 parts by weight of sodium stearate, 3 parts of carboxymethyl cellulose, 7 parts of sodium dodecylbenzene sulfonate solution and 3 parts of citric acid.

[0155] The mass fraction of the sulfuric acid solution in this embodiment is 4%; the mass fraction of the sodium dodecylbenzene sulfonate solution is 7%.

[0156] The ultrasonic power of the immersion ultrasonic treatment in this embodiment is 480W, and the ultrasonic treatment is performed for 1 hour.

[0157] A method for preparing a microecological preparation for reconstructing intestinal microecology in this embodiment comprises the following steps:

[0158] The probiotics, prebiotics, postbiotics, montmorillonite modifiers and nano-silica-modified adjusting agents are fully blended to obtain a microecological preparation for rebuilding the intestinal microecology.

[0159] The present invention also provides an application of a microecological preparation for rebuilding intestinal microecology in preventing and assisting in treating intestinal flora imbalance.

[0160] Comparative Example 1.

[0161] The difference from Example 3 is that no modifier for doping montmorillonite is added.

[0162] Comparative Example 2.

[0163] The difference from Example 3 is that no hydroxyapatite liquid is added in the preparation of the montmorillonite-doped modifier.

[0164] Comparative Example 3.

[0165] Different from Example 3, no montmorillonite agent was added in the preparation of the montmorillonite-doped modifier.

[0166] Comparative Example 4.

[0167] The difference from Example 3 is that no auxiliary agent based on nano-silicon dioxide modification is added.

[0168] Comparative Example 5.

[0169] The difference from Example 3 is that no modifying liquid is added in the preparation of the auxiliary adjusting agent based on nano-silicon dioxide modification.

[0170] The products of Examples 1-3 and Comparative Examples 1-5 were added to the simulated intestinal flora, and the total number of viable bacteria of the composite flora was measured first, and then the flora number was measured after 24h and 48h. The balance rate of the flora was tested by the total number of viable bacteria of the composite flora-24h, 48h flora number performance / total number of viable bacteria of the composite flora. The test results are as follows

[0171]

[0172]

[0173] It can be concluded from Examples 1-3 and Comparative Examples 1-5 that the product of Example 3 has an excellent balance rate of the bacterial flora, and without adding a modifier doped with montmorillonite and without adding one of the auxiliary adjusting agents modified based on nano-silica, the performance of the product deteriorates significantly, and without adding hydroxyapatite liquid in the preparation of the modifier doped with montmorillonite, without adding montmorillonite agent in the preparation of the modifier doped with montmorillonite, and without adding modifying liquid in the preparation of the auxiliary adjusting agent modified based on nano-silica, the performance of the product tends to deteriorate to varying degrees. The performance effect of the product is most significant when the specific raw materials of the present invention are used.

[0174] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

[0175] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A microecological preparation for rebuilding intestinal microecology, characterized in that: The microecological preparation comprises the following raw materials in parts by weight: 8 to 12 parts of probiotics, 3 to 5 parts of prebiotics, and 2 to 4 parts of postbiotics; The microecological preparation further comprises 5 to 8 parts of a modifier doped with montmorillonite and 3 to 4 parts of an auxiliary adjusting agent modified by nano-silicon dioxide.

2. A microecological preparation for rebuilding intestinal microecology according to claim 1, characterized in that: The probiotics are selected from lactic acid bacteria and bifidobacteria, and have the functions of regulating intestinal flora and enhancing immunity; The prebiotic is selected from one of oligofructose and oligogalactose to promote the growth of probiotics; Postbiotics are selected from short-chain fatty acids and bacteriocins, and have antibacterial and anti-inflammatory effects.

3. A microecological preparation for rebuilding intestinal microecology according to claim 1, characterized in that: The preparation method of the modifier doped with montmorillonite is: S01: adding montmorillonite to a sodium alginate solution of 3-5 times the total amount of montmorillonite, and then adding a phosphate buffer solution of 5-10% of the total amount of montmorillonite, stirring sufficiently to obtain a montmorillonite agent; S02: adding 2-3 parts of hydroxyapatite and 1-3 parts of nano-silica sol to 2-4 parts of chitosan solution, and stirring thoroughly to obtain hydroxyapatite liquid; S03: Mix the montmorillonite agent and the hydroxyapatite liquid in a weight ratio of 5:3 and perform ball milling at a ball milling speed of 1000-1500 r / min for 1 hour. After the ball milling is completed, filter and dry to obtain a modifier doped with montmorillonite.

4. A microecological preparation for rebuilding intestinal microecology according to claim 3, characterized in that: The mass fraction of the sodium alginate solution is 2-5%; the mass fraction of the chitosan solution is 3-6%.

5. A microecological preparation for rebuilding intestinal microecology according to claim 3, characterized in that: The pH value of the phosphate buffer solution is 5.

0.

6. The microecological preparation for rebuilding intestinal microecology according to claim 1, characterized in that: The preparation method of the nano-silicon dioxide-modified auxiliary agent is as follows: S101: adding nano-silicon dioxide into sulfuric acid solution in a weight ratio of 2:7 and stirring thoroughly, then washing with water and drying; Preheating the dried nano-silicon dioxide to 65-70° C. for 1 hour to obtain preheated nano-silicon dioxide; S102: immersing the preheated nano-silicon dioxide into the modified liquid for ultrasonic treatment, filtering and drying after the immersion is completed, to obtain an auxiliary agent modified by the nano-silicon dioxide; The modified liquid comprises 2-5 parts by weight of sodium stearate, 1-3 parts of carboxymethyl cellulose, 5-8 parts of sodium dodecylbenzene sulfonate solution and 2-3 parts of citric acid.

7. A microecological preparation for rebuilding intestinal microecology according to claim 6, characterized in that: The mass fraction of the sulfuric acid solution is 2-5%; the mass fraction of the sodium dodecylbenzene sulfonate solution is 4-8%.

8. The microecological preparation for rebuilding intestinal microecology according to claim 6, characterized in that: The ultrasonic power of the immersion ultrasonic treatment is 400-500W, and the ultrasonic treatment is performed for 1 hour.

9. A method for preparing a microecological preparation for reconstructing intestinal microecology according to any one of claims 1 to 8, characterized in that: The following steps are involved: The probiotics, prebiotics, postbiotics, montmorillonite modifiers and nano-silica-modified adjusting agents are fully blended to obtain a microecological preparation for rebuilding the intestinal microecology.

10. Use of a probiotic preparation for rebuilding intestinal microecology as claimed in any one of claims 1 to 8 in preventing and assisting in treating intestinal flora imbalance.

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