A soothing composition, its uses, and cosmetics

Abstract

This application belongs to the field of cosmetics and discloses a soothing composition and its uses. The soothing composition is composed of Scutellaria baicalensis root extract, Ophiopogon japonicus root extract and Cornus officinalis fruit extract, or Scutellaria baicalensis root extract, Ophiopogon japonicus root extract and Tribulus terrestris fruit extract, or Scutellaria baicalensis root extract, Ophiopogon japonicus root extract, Cornus officinalis fruit extract and Tribulus terrestris fruit extract. By using the above three or four plant extracts in combination, the soothing effect of the soothing composition can be synergistically enhanced. In addition, this application also discloses a cosmetic containing 0.1-30 wt% of the above soothing composition.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and particularly to a soothing composition and its uses, as well as cosmetics. Background Art

[0002] In today's fast-paced living environment, people are facing pressures from various aspects such as work and life. This not only affects the mental state but also manifests physically, such as skin sensitivity, muscle tension and other problems becoming more common. The soothing composition emerges as the times require, aiming to relieve the discomfort faced by the body and skin through the combination of specific ingredients.

[0003] From the perspective of skin care, environmental pollution, excessive skin care, and improper use of cosmetics often lead to damage to the skin barrier, resulting in sensitive symptoms such as flushing, itching, and dryness. Traditional skin care products are difficult to fundamentally solve these problems, while the soothing composition adds a variety of ingredients with soothing effects, which can effectively reduce the skin inflammatory response and relieve skin discomfort.

[0004] For example, Chinese Patent Application No. 202311718262.7 discloses a skin care composition containing lotus extract and its preparation method. The skin care composition contains lotus extract, cactus extract, oat bran extract, ophiopogon root extract, peony root extract, and scutellaria root extract. Through this scheme, it can be known that the ophiopogon root extract contains various active ingredients such as sugars, amino acids, and saponins, and the scutellaria root extract is rich in various active ingredients such as flavonoids and baicalein, and has good soothing effects.

[0005] However, through the research of this application, it is found that when the scutellaria root extract and the ophiopogon root extract form a composition, although it has certain soothing effects, there is still room for further enhancement.

[0006] The problem to be solved by this scheme: How to improve the soothing effects of the scutellaria root extract and the ophiopogon root extract. Summary of the Invention

[0007] To solve the above problems, the object of the present invention is to provide a soothing composition and its uses, as well as cosmetics. The soothing composition of the present invention inhibits key inflammatory signaling pathways such as NF-κB and MAPK through different pathways, synergistically reduces the release of inflammatory mediators, and enhances the soothing and repair effects of cosmetics through multi-target and multi-pathway synergistic effects.

[0008] To achieve the above object, the present application discloses a soothing composition, which is composed of scutellaria root extract, ophiopogon root extract, and cornel fruit extract, and the composition is obtained by compound extraction with 0.5 - 5 parts by weight of scutellaria root, 1 - 7 parts by weight of ophiopogon root, and 2 - 9 parts by weight of cornel fruit as raw materials;

[0009] Or the composition is composed of a scutellaria root extract, an ophiopogon root extract and a tribulus terrestris fruit extract, and the composition is obtained by composite extraction of 0.5 to 5 parts by weight of scutellaria root, 1 to 7 parts by weight of ophiopogon root and 1 to 7 parts by weight of tribulus terrestris fruit as raw materials;

[0010] Or the composition is composed of a scutellaria root extract, an ophiopogon root extract, a cornus officinalis extract and a tribulus terrestris fruit extract, and the composition is obtained by composite extraction of 0.5 to 5 parts by weight of scutellaria root, 1 to 7 parts by weight of ophiopogon root, 2 to 9 parts by weight of cornus officinalis and 1 to 7 parts by weight of tribulus terrestris fruit as raw materials;

[0011] Scutellaria root extract contains flavonoids and polysaccharides with strong antioxidant capacity, which can inhibit the production of free radicals, reduce skin oxidative damage, enhance skin barrier capacity, reduce water loss, thereby enhancing the skin's self-protection ability and reducing sensitivity;

[0012] Ophiopogon root extract can downregulate the expression of inflammatory factors (such as IL-6, IL-8 and IL-1β) and inhibit inflammatory response. It is also rich in polysaccharides, saponins and isoflavones, which can scavenge free radicals and reduce oxidative damage. Ophiopogon root extract can also regulate skin immune function, promote the synthesis of natural moisturizing factor (NMF), increase the hydration of the stratum corneum, and enhance the stability of the skin barrier, thereby improving the overall health of the skin.

[0013] Cornus officinalis extract contains active ingredients such as iridoid glycosides and triterpenoid compounds, which can reduce skin inflammatory reactions by inhibiting the release of inflammatory mediators and the expression of inflammatory factors. It also contains ingredients such as polysaccharides, flavonoids and organic acids, which have good antioxidant capacity, can scavenge free radicals, reduce oxidative damage, protect the skin from external environmental damage, and can also inhibit the activity of hyaluronidase and reduce the release of histamine and β-hexosaminidase, thereby effectively alleviating allergic reactions.

[0014] Tribulus terrestris fruit extract can inhibit the release of inflammatory mediators and the expression of inflammatory factors, thereby reducing skin inflammatory reactions; at the same time, Tribulus terrestris fruit extract is also rich in antioxidant ingredients, such as polyphenol compounds, which can quickly react with free radicals after they are generated, remove them, and reduce the damage of free radicals to the skin; further, Tribulus terrestris fruit extract can also promote the proliferation of keratinocytes, increase the content of keratin and ceramide, enhance the structural integrity of the stratum corneum, and maintain the moisture barrier of the skin, thereby improving the barrier function of the skin;

[0015] The Scutellaria baicalensis Georgi root extract reduces the inflammatory response by inhibiting inflammatory mediators (such as COX-2, PGE2) and regulating the NF-κB signaling pathway. The Ophiopogon japonicus root extract inhibits the release of inflammatory factors (such as TNF-α, IL-6) by regulating the TLR4 / NF-κB signaling pathway. The Cornus officinalis extract also reduces the inflammatory response by inhibiting the MAPK and NF-κB signaling pathways. The Tribulus terrestris fruit extract exerts an anti-inflammatory effect by inhibiting the generation of inflammatory mediators (such as NO, PGE2) and regulating the activity of immune cells. The four extracts inhibit key inflammatory signaling pathways such as NF-κB and MAPK through different pathways, synergistically reducing the release of inflammatory mediators, and enhancing the soothing and repairing effects of cosmetics through multi-target and multi-pathway synergistic effects.

[0016] Preferably, the composite extraction method of the soothing composition is as follows:

[0017] Step 1: Crush the raw materials into powders, mix them and add them to ethanol with a volume fraction of 50% according to a mass ratio of 1-2:10, stir at 55±3°C for 3 h, centrifuge, and concentrate at 60±3°C until the ethanol content is zero to obtain a non-alcoholic concentrated solution.

[0018] Step 2: Mix the non-alcoholic concentrated solution, butylene glycol and water according to a mass ratio of 2-5:3:2-5, stir and extract for 0.5 h, and perform ultrafiltration at 0.25 MPa to obtain a filtrate.

[0019] Step 3: Add a preservative to the filtrate, sterilize at 85±5°C for 0.8-1.2 h to obtain the soothing composition.

[0020] Preferably, the preservative in Step 3 is selected from at least one of p-hydroxyacetophenone and 1,2-hexanediol.

[0021] In addition, the use of the above-mentioned soothing composition in the preparation of cosmetics is also disclosed.

[0022] In addition, a cosmetic is also disclosed, and the cosmetic contains 0.1-30 wt% of the above-mentioned soothing composition.

[0023] Preferably, the cosmetic is one of an aqueous solution, an essence, an essence milk, a facial mask liquid and a cream.

[0024] The beneficial effects of the present invention are:

[0025] The present invention provides a soothing composition, its use and a cosmetic. The Scutellaria baicalensis Georgi root extract and Ophiopogon japonicus root extract with good antioxidant effects are combined with the Cornus officinalis extract and Tribulus terrestris fruit extract with good anti-inflammatory effects. They inhibit key inflammatory signaling pathways such as NF-κB and MAPK through different pathways, synergistically reduce the release of inflammatory mediators, and enhance the soothing and repairing effects of cosmetics through multi-target and multi-pathway synergistic effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Observation diagram of chicken embryo before using the soothing composition in Example 3 of the present invention;

[0027] Figure 2 Observation diagram of chicken embryo after using the soothing composition in Example 3 of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0028] In the description of the present invention, it should be noted that for those not specified in the examples, they are carried out according to conventional conditions or conditions recommended by the manufacturer. For reagents or instruments whose manufacturers are not specified, they are all conventional products that can be obtained by purchasing in the market.

[0029] Supplier information and / or product information

[0030] Radix Scutellariae: Purchased from Bozhou Feimao Pharmaceutical Co., Ltd., with a water content of 8.4%;

[0031] Radix Ophiopogonis: Purchased from Bozhou Feimao Pharmaceutical Co., Ltd., with a water content of 14.3%;

[0032] Fructus Corni: Purchased from Bozhou Bozhongtang Pharmacy Co., Ltd., with a water content of 12.1%;

[0033] Fructus Tribuli: Purchased from Bozhou Bozhongtang Pharmacy Co., Ltd., with a water content of 6.2%.

[0034] In the following examples and comparative examples, the extraction method of the soothing composition is as follows:

[0035] Step 1: Crush the raw materials into powder, mix them and add them to ethanol with a volume fraction of 50% according to a mass ratio of 1.5:10, stir at 55°C for 3 h, centrifuge, and concentrate at 60°C until the ethanol content is zero to obtain a non-alcohol concentrated solution;

[0036] Step 2: Mix the non-alcohol concentrated solution, butylene glycol and water according to a mass ratio of 5:3:2, stir and extract for 0.5 h, and perform ultrafiltration at 0.25 MPa to obtain a filtrate;

[0037] Step 3: Add 0.5% of p-hydroxyacetophenone based on the mass of the filtrate and 0.5% of 1,2-hexanediol based on the mass of the filtrate to the filtrate, and sterilize at 85°C for 1 h to obtain the soothing composition;

[0038] Among them, the crushed Radix Scutellariae and Fructus Tribuli pass through a 24-mesh sieve, and the crushed Fructus Corni and Radix Ophiopogonis pass through a 50-mesh sieve.

[0039] Examples 1-5

[0040] A soothing composition, calculated by mass parts, its component composition is shown in Table 1:

[0041] Table 1

[0042] Group Scutellaria baicalensis Georgi root powder Ophiopogon japonicus root powder Cornus officinalis powder Tribulus terrestris fruit powder Example 1 2 4 5 0 Example 2 2 4 0 4 Example 3 2 4 5 4 Example 4 0.5 7 2 7 Example 5 5 1 9 1

[0043] Comparative Examples 1 - 4

[0044] A soothing composition, by mass, its component composition is shown in Table 2:

[0045] Table 2

[0046] Group Scutellaria baicalensis Georgi root powder Ophiopogon japonicus root powder Cornus officinalis powder Tribulus terrestris fruit powder Control Example 1 2 4 0 0 Control Example 2 0 0 5 0 Control Example 3 0 0 0 4 Control Example 4 0 0 5 4

[0047] Comparative Examples 5 - 8

[0048] A soothing composition, by mass, its component composition is shown in Table 3:

[0049] Table 3

[0050] Group Scutellaria baicalensis Georgi root powder Ophiopogon japonicus root powder Cornus officinalis powder Tribulus terrestris fruit powder Other components Control Example 5 2 4 5 0 4 Control Example 6 2 4 0 4 5 Control Example 7 0 0 0 0 4 Control Example 8 0 0 0 0 5

[0051] Among them, the other component in Comparative Example 5 is Dictamnus dasycarpus Turcz. powder;

[0052] The other component in Comparative Example 6 is Magnolia officinalis Rehd. et Wils. root powder;

[0053] The other component in Comparative Example 7 is Dictamnus dasycarpus Turcz. powder;

[0054] The other component in Comparative Example 8 is Magnolia officinalis Rehd. et Wils. root powder;

[0055] And the Dictamnus dasycarpus Turcz. powder and Magnolia officinalis Rehd. et Wils. root powder in Comparative Examples 5 - 8 are respectively obtained by crushing commercially available Dictamnus dasycarpus Turcz. and Magnolia officinalis Rehd. et Wils. root.

[0056] Performance test:

[0057] I. Detection of the content of inflammatory factor IL - 6 in macrophages

[0058] 1 Test purpose and principle

[0059] Macrophages can be used as a cell model to study the inhibition of the content of inflammatory factor IL - 6 by cosmetics. By stimulating with lipopolysaccharide (LPS), the content of inflammatory factor IL - 6 in the negative control and the test sample group is measured to evaluate whether the test sample has efficacy in inhibiting the content of inflammatory factor IL - 6. The content of IL - 6 is measured by enzyme - linked immunosorbent assay (ELISA).

[0060] In this test, referring to the laboratory method, the results of the determination of the content of cell IL - 6 inflammatory factor of the test sample and the negative control are compared. If the content of cell IL - 6 inflammatory factor of the test sample is lower than that of the negative control and has a significant difference (P < 0.05), it can be considered that the test sample has a certain soothing effect.

[0061] 2 Experimental Materials and Methods

[0062] 2.1 Instrument and Equipment: RT-6100 Microplate Reader.

[0063] 2.2 Macrophages Used in the Experiment: RAW264.7 cells.

[0064] 2.3 Experimental Methods

[0065] (1) Treatment of Test Samples

[0066] Sample Group: Dilute the compositions prepared in Examples 1-5 and Comparative Examples 1-8 with pure water to a solution with a composition concentration of 0.5%.

[0067] Negative Control: Basic culture medium containing LPS.

[0068] (2) Experimental Operation Steps

[0069] Determination of IL-6 Inflammatory Factor Content: Seed RAW264.7 cells in a 96-well plate. After culturing for 24 h, replace the medium with basic medium containing test samples at different concentrations. After 24 h, collect the cell supernatant and centrifuge. Aspirate the supernatant and use an ELISA kit to determine the IL-6 content in the cell culture supernatant, so as to obtain the effect of the test samples on the IL-6 content of macrophages.

[0070] (3) Calculation Formula

[0071] Equation 1.

[0072] (4) Data Analysis

[0073] The statistical analysis software is SPSS. The independent samples t-test is used for comparison between the test samples and the negative control. The above statistical analyses are all two-tailed tests, and the significance level is α = 0.05. P > 0.05 indicates no significant difference between the two groups; P < 0.05 indicates a significant difference between the two groups.

[0074] The test results are shown in Table 4:

[0075] Table 4 Test Results

[0076] Group Relative content of IL-6 (%) P value Group Relative content of IL-6 (%) P value Example 1 60.79 <0.05 Control Example 3 92.41 <0.05 Example 2 61.05 <0.05 Control Example 4 83.10 <0.05 Example 3 51.22 <0.05 Control Example 5 60.25 <0.05 Example 4 53.31 <0.05 Control Example 6 60.06 <0.05 Example 5 53.57 <0.05 Control Example 7 91.23 <0.05 Control Example 1 75.18 <0.05 Control Example 8 91.68 <0.05 Control Example 2 90.63 <0.05 Negative control 100 /

[0077] Result Analysis:

[0078] 1. As can be seen from Examples 1-3, when the soothing composition contains fructus corni extract, fructus tribuli extract, radix scutellariae extract and radix ophiopogonis extract at the same time, the inhibitory effect on the inflammatory factor IL-6 is the best.

[0079] 2. As can be seen from Examples 3 - 5, when the soothing composition is obtained by extracting Cornus officinalis powder, Tribulus terrestris fruit powder, Scutellaria baicalensis root powder, and Ophiopogon japonicus root powder in a mass ratio of 2:4:5:4, the inhibitory effect on the inflammatory factor IL - 6 is the best.

[0080] 3. As can be seen from Example 1 and Comparative Examples 1 - 2, when the soothing composition contains Scutellaria baicalensis root extract, Ophiopogon japonicus root extract, and Cornus officinalis extract, the relative content of the inflammatory factor IL - 6 should be 65.81%. However, looking at Example 1, the relative content of this composition for the inflammatory factor IL - 6 is 60.79%, which is less than 65.81%. Thus, it can be known that there is a synergistic effect between Cornus officinalis extract and Scutellaria baicalensis root extract, and Ophiopogon japonicus root extract;

[0081] Furthermore, as can be seen from Example 2 and Comparative Examples 1 and 3, when the soothing composition contains Scutellaria baicalensis root extract, Ophiopogon japonicus root extract, and Tribulus terrestris fruit extract, its relative content for the inflammatory factor IL - 6 should be 67.59%. However, looking at Example 2, the relative content of the inflammatory factor IL - 6 in this composition is 61.05%, which is less than 67.59%. Thus, it can be known that there is also a synergistic effect between Tribulus terrestris fruit extract and Scutellaria baicalensis root extract, and Ophiopogon japonicus root extract, and the synergistic effect of Tribulus terrestris fruit extract on Scutellaria baicalensis root extract and Ophiopogon japonicus root extract is stronger than that of Cornus officinalis extract;

[0082] Furthermore, as can be seen from Comparative Examples 2 - 4, there is also a synergistic effect between Cornus officinalis extract and Tribulus terrestris fruit extract;

[0083] Finally, as can be seen from Example 3 and Comparative Examples 1 - 3, the relative content of the inflammatory factor IL - 6 in Example 3 is 51.22%, which is less than the theoretical relative content of 58.22%. It is speculated that this is because Cornus officinalis extract and Tribulus terrestris fruit extract both have a synergistic effect on Scutellaria baicalensis root extract and Ophiopogon japonicus root extract, and there is also a synergistic effect between the former two, so that multiple synergistic effects work together to further reduce the relative content of the inflammatory factor IL - 6 in the composition.

[0084] 4. As can be seen from Example 3 and Comparative Examples 5 - 8, the soothing composition obtained by extracting Dictamnus dasycarpus Turcz. powder and Magnolia officinalis root powder commercially available also has a certain inhibitory effect on the inflammatory factor IL - 6. However, when they are respectively used to replace Cornus officinalis powder and Tribulus terrestris fruit powder for extraction, the relative content of the inflammatory factor IL - 6 in the obtained composition is within the theoretical range, and there is no synergistic effect. Thus, it can be known that Cornus officinalis extract, Tribulus terrestris fruit extract, Scutellaria baicalensis root extract, and Ophiopogon japonicus root extract are irreplaceable with each other.

[0085] II. Hyaluronidase Inhibition Rate Test

[0086] 1 Test Purpose and Principle

[0087] Hyaluronidase is a specific lyase for hyaluronic acid and is a participant in allergic reactions, showing a strong correlation with the release of histamine by mast cells. Whether a test sample has a soothing effect can be judged by the hyaluronidase inhibition rate. The higher the hyaluronidase inhibition rate, the stronger the soothing effect of the substance; conversely, the weaker the effect.

[0088] In this experiment, the hyaluronidase inhibition rate test results of the test sample were compared with those of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and there is a significant difference (P < 0.05), it can be considered that the test sample has a certain soothing effect.

[0089] 2 Test Materials and Methods

[0090] 2.1 Instrument and Equipment: BSA224S Analytical Balance, L6s UV-Visible Spectrophotometer.

[0091] 2.2 Reagents: BR-grade hyaluronidase, BR-grade sodium hyaluronate.

[0092] 2.3 Test Method

[0093] (1) Treatment of Control and Test Samples

[0094] Sample Group: Dilute the compositions prepared in Examples 1 - 5 and Comparative Examples 1 - 8 with pure water to a composition concentration of 5%;

[0095] Positive Control (Dipotassium Glycyrrhizinate, purity ≥ 98%): Dilute with water to a positive control concentration of 3%;

[0096] Negative Control: Pure water.

[0097] (2) Test Operation Steps

[0098] Set up a sample group, a sample background group, a solvent group, and a solvent background group. Each group needs to have 3 parallels. Add different reagent solutions to the four groups respectively, shake well, let stand at room temperature for 30 min for color development, and measure the absorbance value at a wavelength of 528 nm using a UV-visible spectrophotometer.

[0099] (3) Calculation Formula

[0100] Equation 2.

[0101] In the formula:

[0102] A — is the absorbance of the reaction solution without the sample;

[0103] B — is the absorbance of the reaction solution without the sample and the enzyme;

[0104] C — is the absorbance of the reaction solution containing the sample and the enzyme;

[0105] D is the absorbance of the reaction solution containing the sample and without enzyme.

[0106] (4) Data analysis

[0107] The statistical analysis software is SPSS. The independent samples t-test is used to compare the hyaluronidase inhibition rates among the test samples, positive control substances, and negative control substances. The above statistical analyses are all two-tailed tests, and the significance level is a = 0.05. P ≥ 0.05 indicates no significant difference between the two groups; P < 0.05 indicates a significant difference between the two groups.

[0108] The test results are shown in Table 5:

[0109] Table 5 Test Results

[0110] Group Hyaluronidase inhibition rate / % P value Group Hyaluronidase inhibition rate / % P value Example 1 41.912 <0.05 Control Example 4 19.194 <0.05 Example 2 42.129 <0.05 Control Example 5 37.798 <0.05 Example 3 58.621 <0.05 Control Example 6 38.713 <0.05 Example 4 56.763 <0.05 Control Example 7 9.176 <0.05 Example 5 56.844 <0.05 Control Example 8 9.384 <0.05 Control Example 1 21.785 <0.05 Positive control group 68.338 <0.05 Control Example 2 9.423 <0.05 Negative control group -0.528 / Control Example 3 12.965 <0.05

[0111] Result analysis:

[0112] 1. As can be seen from Examples 1 - 3, when the soothing composition contains Cornus officinalis extract, Tribulus terrestris fruit extract, Scutellaria baicalensis root extract, and Ophiopogon japonicus root extract at the same time, the inhibitory effect on hyaluronidase is the best.

[0113] 2. As can be seen from Examples 3 - 5, when the soothing composition is obtained by extracting Cornus officinalis powder, Tribulus terrestris fruit powder, Scutellaria baicalensis root powder, and Ophiopogon japonicus root powder in a mass ratio of 2:4:5:4, the inhibitory effect on hyaluronidase is the best.

[0114] 3. As can be seen from Example 1 and Comparative Examples 1 - 2, when the soothing composition contains Scutellaria baicalensis root extract, Ophiopogon japonicus root extract, and Cornus officinalis extract, its inhibition rate of hyaluronidase should be 31.208%. However, looking at Example 1, the inhibition rate of this composition on hyaluronidase is 41.912%, which is much greater than 31.208%. From this, it can be seen that there is a synergistic effect between Cornus officinalis extract and Scutellaria baicalensis root extract, and Ophiopogon japonicus root extract;

[0115] Also, as can be seen from Example 2 and Comparative Examples 1 and 3, when the soothing composition contains Scutellaria baicalensis root extract, Ophiopogon japonicus root extract, and Tribulus terrestris fruit extract, its inhibition rate of hyaluronidase should be 34.750%. However, looking at Example 2, the inhibition rate of this composition on hyaluronidase is 42.129%, which is much greater than 34.750%. From this, it can be seen that there is also a synergistic effect between Tribulus terrestris fruit extract and Scutellaria baicalensis root extract, and Ophiopogon japonicus root extract;

[0116] However, as can be seen from Comparative Examples 2 - 4, there is no synergistic effect between Tribulus terrestris fruit extract and Cornus officinalis extract in terms of hyaluronidase inhibition;

[0117] Finally, it can be seen from Example 3 and Comparative Examples 1-3 that the inhibition rate of Example 3 on hyaluronidase, 58.621%, is much greater than the theoretical inhibition rate of 40.173%. It is speculated that this is because the extracts of Cornus officinalis and Tribulus terrestris have a synergistic effect on the extracts of Scutellaria baicalensis root and Ophiopogon japonicus root, and there is also a synergistic effect between the former two. Thus, the combined action of multiple synergistic effects further enhances the inhibitory effect of the composition on hyaluronidase.

[0118] 4. It can be seen from Example 3 and Comparative Examples 5-8 that the soothing composition extracted from commercially available Dictamnus dasycarpus powder and Magnolia officinalis root powder also has a certain inhibitory effect on hyaluronidase. However, when they are used to replace Cornus officinalis powder and Tribulus terrestris fruit powder for extraction respectively, the inhibitory effect of the resulting composition on hyaluronidase is within the theoretical range, and there is no synergistic effect. Therefore, it can be known that the extracts of Cornus officinalis, Tribulus terrestris fruit, Scutellaria baicalensis root, and Ophiopogon japonicus root cannot be replaced with each other.

[0119] III. Irritation

[0120] Test method: Chicken embryo chorioallantoic membrane test.

[0121] Treatment and reagents for test groups

[0122] Sample: Dilute the soothing compositions prepared in Examples 1-5 and Comparative Examples 1-8 to 2% with pure water;

[0123] Negative control: 0.9% normal saline;

[0124] Positive control: 1% sodium dodecyl sulfate solution.

[0125] Test steps

[0126] In this test, 6 chicken embryos are selected for each group. Use a photographing device to record the situation of the chorioallantoic membrane. Place a polytetrafluoroethylene resin ring on the chorioallantoic membrane of the chicken embryo and take a photo for recording. Add the test sample into the polytetrafluoroethylene resin ring, record the time of adding the sample, cover the air chamber with a moistened plastic wrap, move the chicken embryo to an incubator with constant temperature and humidity, and observe the degree of change of each toxic effect.

[0127] Result observation

[0128] Observe and record the manifestations of bleeding, blood coagulation, and vascular lysis, and score according to their severity. The scoring criteria for bleeding, blood coagulation, and vascular lysis are shown in Table 6:

[0129] Table 6 Scoring criteria

[0130]

[0131] Result calculation

[0132] The test was carried out using the endpoint evaluation method, and the endpoint evaluation (ES) was calculated, with the result reserved to two decimal places; the score of each chick embryo was the sum of the bleeding, coagulation, and vascular lysis degrees observed in each chick embryo; ES was the average value of the mathematical sum obtained from 6 chick embryos.

[0133] The average score of ES was calculated according to the following formula:

[0134] Formula 3.

[0135] Result judgment criteria:

[0136] ES ≤ 4, non-irritating; 4 < ES ≤ 12, slightly irritating; 12 < ES < 16, moderately irritating; ES ≥ 16, strongly irritating / corrosive.

[0137] According to the test, neither the examples nor the comparative examples produced irritation. Refer to Figure 1 、 Figure 2 , for the comparison of the chick embryo states before and after dropping the solution prepared from the composition of Example 3. After statistics, the ES value of the solution prepared from the composition of Example 3 was 2.33.

[0138] Application example

[0139] A soothing essence containing the soothing composition of Example 3, and the formulation ratio is shown in Table 7.

[0140] Table 7 Formulation ratio of the soothing essence

[0141]

[0142] Its preparation method is as follows:

[0143] Step 1: Dissolve Phase B in advance and stir to mix evenly; pre-mix and stir Phase C evenly for later use;

[0144] Step 2: Add the pure water in Phase A to the pot, start homogenization, and sequentially add the remaining raw materials in Phase A under the homogenization state. Homogenize at 3000 - 4000 rpm for 30 - 60 s, stir and heat to 80 - 85 °C, homogenize at 3000 - 4000 rpm for 1 - 2 min until there are no gel particles, keep warm for 30 min and then stir to cool down;

[0145] Step 3: Cool down to 50 °C, sequentially add Phase B and Phase C, and stir evenly;

[0146] Step 4: Cool down to 45 °C, add Phase D, and stir evenly.

[0147] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A soothing composition, characterized in that, The soothing composition is obtained by compound extraction from 0.5 to 5 parts by weight of Scutellaria baicalensis Georgi root, 1 to 7 parts by weight of Ophiopogon japonicus root, 2 to 9 parts by weight of Cornus officinalis Sieb. et Zucc., and 1 to 7 parts by weight of Tribulus terrestris L. fruit. The compound extraction method of the soothing composition is as follows: Step 1: Crush the raw materials into powders, mix them and add them to ethanol with a volume fraction of 50% at a mass ratio of 1 to 2:10, stir at 55 ± 3 °C for 3 h, centrifuge, and concentrate at 60 ± 3 °C until the ethanol content is zero to obtain a non-alcoholic concentrated solution; Step 2: Mix the non-alcoholic concentrated solution, butylene glycol and water at a mass ratio of 2 to 5:3:2 to 5, stir and extract for 0.5 h, and perform ultrafiltration at 0.25 MPa to obtain a filtrate; Step 3: Add a preservative to the filtrate, sterilize at 85 ± 5 °C for 0.8 to 1.2 h to obtain the soothing composition.

2. The soothing composition according to claim 1, wherein The preservative in Step 3 is selected from at least one of p-hydroxyacetophenone and 1,2-hexanediol.

3. Use of the soothing composition according to claim 1 or 2 in the preparation of cosmetics.

4. A cosmetic, characterized in that, The cosmetics contain 0.1 to 30 wt% of the soothing composition according to claim 1 or 2.

5. The cosmetic according to claim 4, characterized in that, The cosmetics are one of aqueous solutions, essence, essence milk, facial mask liquid and cream.

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