Cordycepin-containing composition for improving sperm quality, pharmaceutical preparation and application

Abstract

The invention discloses a cordycepin-containing composition for improving sperm quality, a pharmaceutical preparation and application, the cordycepin-containing composition is prepared from the following raw materials: 1-5 parts of cordyceps militaris extract, 1-15 parts of morchella, 1-15 parts of mushroom, 1-15 parts of cistanche, 1-5 parts of cynomorium songaricum, 1-10 parts of wolfberry, 1-10 parts of schisandra chinensis, 1-10 parts of semen cuscutae, 1-10 parts of teasel root, 1-10 parts of eucommia ulmoides and 1-10 parts of poria cocos, and edible auxiliary materials can also be matched. By utilizing the principle of homology of medicine and food, traditional Chinese medicinal materials are developed into functional food, cordycepin, adenosine and cordyceps militaris polysaccharide in cordyceps militaris are separated through an extraction technology, and the cordyceps militaris extract is added into the composition as one of active substances, so that the prepared medicinal preparation can remarkably improve the sperm density, effectively reduce the deformity rate and improve the sperm quality. Therefore, the sperm quality is improved.

Description

Technical Field

[0001] The invention relates to the field of health food, and in particular to a cordycepin-containing composition and a pharmaceutical preparation and use thereof for improving sperm quality. Background Art

[0002] Nowadays, men are facing a lot of pressure and pollution in their living environment, such as the pressure caused by fierce social competition, pressure from life such as mortgage and car loans, as well as pollution in air and food, or due to certain diseases (such as nervous system diseases, reproductive system diseases), etc., a series of male sexual dysfunction and damage, mainly manifested in low libido, premature ejaculation, impotence, spermatorrhea, anejaculation and retrograde ejaculation. According to literature reports, the average incidence of sexual dysfunction in Chinese men aged 40-69 is 47.3%. Some of the functional health products for strengthening yang and tonifying kidney on the market have added prescription drugs such as sildenafil. After use, symptoms such as night sweats, dizziness, nausea, and vomiting will occur. Overdose can cause abnormal erection of the penis, causing permanent damage, sudden loss of vision in one or both eyes, sudden hearing loss or loss, and sudden drop in blood pressure. The popular foods such as leeks, oysters, and kidneys have little effect on strengthening yang after being decomposed and absorbed by the digestive tract and stomach, and can only play a psychological role.

[0003] At present, Cordyceps militaris or Cordyceps sinensis oral liquid and its series of preparations do not use the active substances in their bodies such as cordycepin as raw materials, but use Cordyceps militaris powder or Cordyceps sinensis powder to explain the overall efficacy, which has certain limitations. Cordycepin is one of the active substances in Cordyceps sinensis, Cordyceps militaris and other Cordyceps fungi. Its anti-inflammatory, antibacterial, antiviral and anti-tumor effects have been confirmed by more and more clinical and basic experiments. However, its therapeutic effect on gonadotropin development has not been reported. Summary of the invention

[0004] The purpose of the present invention is to provide a cordycepin-containing composition and a pharmaceutical preparation and use for improving sperm quality, which can significantly increase sperm density, effectively reduce deformity rate, and thus improve sperm quality.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] A cordycepin composition for improving sperm quality, the composition comprising the following components in parts by weight: 1-5 parts of Cordyceps militaris extract, 1-15 parts of Morchella, 1-15 parts of Lentinula edodes, 1-15 parts of Cistanche deserticola, 1-5 parts of Cynomorium songaricum, 1-10 parts of Lycium barbarum, 1-10 parts of Schisandra chinensis, 1-10 parts of Cuscuta australis, 1-10 parts of Dipsacus asper, 1-10 parts of Eucommia ulmoides and 1-10 parts of Poria; in the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is in the range of 50wt% to 60wt%, the content of adenosine is in the range of 10wt% to 20wt%, and the content of Cordyceps militaris polysaccharide is in the range of 20wt% to 30wt%.

[0007] Furthermore, the composition includes the following components in parts by weight: 2-4 parts of Cordyceps militaris extract, 4-12 parts of Morel, 4-12 parts of Lentinus edodes, 6-12 parts of Cistanche, 2-4 parts of Cynomorium songaricum, 4-8 parts of Lycium barbarum, 4-8 parts of Schisandra chinensis, 4-8 parts of Cuscuta australis, 4-8 parts of Dipsacus asper, 4-8 parts of Eucommia ulmoides and 4-8 parts of Poria.

[0008] Furthermore, the composition also contains 10 to 20 portions of edible auxiliary materials.

[0009] Furthermore, the extraction of cordycepin and adenosine adopts an assisted ultrasonic extraction method, specifically: Cordyceps militaris powder is mixed with 30-80% ethanol in a solid-liquid ratio of 1: (10-40) and placed in an ultrasonic extraction device, the temperature is set to 30-70°C and the frequency is set to 20-80kHz. After ultrasonic extraction for 30-90 minutes, a filtrate is obtained by filtration using a 0.45μm membrane, and the filtrate is dried using a rotary evaporator to obtain a sample.

[0010] Furthermore, the extraction of cordycepin and adenosine adopts an assisted ultrasonic extraction method, specifically: Cordyceps militaris powder is mixed with 40-60% ethanol in a solid-liquid ratio of 1: (10-20) and placed in an ultrasonic extraction device, the temperature is set to 50-60°C and the frequency is 40-60kHz. After ultrasonic extraction for 30-60 minutes, a 0.45μm membrane is used for filtration to obtain a filtrate, and the filtrate is dried using a rotary evaporator to obtain a sample.

[0011] Furthermore, the polysaccharide is extracted by enzymatic hydrolysis, wherein Cordyceps militaris powder is mixed with distilled water at a solid-liquid ratio of 1: (10-30), cellulase, pectinase and papain are added, and after complete dissolution, the mixture is water-bathed at 50-60° C. for 50-70 minutes. After the water-bath is completed, the mixture is heated to 90-100° C. for 10-15 minutes to denature and inactivate the added enzyme; the mixture is then centrifuged at a speed of 6000-8000 r / min for 5-10 minutes to collect the supernatant and concentrated using a rotary evaporator, and after cooling to room temperature, 4-6 times the volume of 95% ethanol is added while stirring, and the mixture is centrifuged at a speed of 6000-8000 r / min for 5-10 minutes to collect the supernatant, and the protein is removed by the Sevag method. After 36-48 hours of dialysis, the solid obtained is freeze-dried to obtain the polysaccharide.

[0012] The invention also provides a pharmaceutical preparation, which contains the above composition.

[0013] Furthermore, the preparation is in the form of tablets, capsules, powders, pills or oral solutions.

[0014] The present invention also provides use of the above composition or pharmaceutical preparation in preparing a drug for improving sperm quality.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention utilizes the principle of medicine and food homology to develop Chinese medicinal materials into functional foods, separates cordycepin, adenosine and Cordyceps militaris polysaccharides in Cordyceps militaris through extraction technology, and adds the Cordyceps militaris extract as one of the active substances into the composition. The prepared pharmaceutical preparation can significantly increase sperm density, effectively reduce deformity rate, and thus improve sperm quality.

[0017] The composition and pharmaceutical preparation of the present invention are based on the cordycepin in the Cordyceps militaris extract as the main active ingredient, supplemented with adenosine and Cordyceps militaris polysaccharide, and cordycepin can improve testosterone levels and improve sperm quality as the main effective ingredient. Adenosine can regulate the internal environment of the body, promote cell energy metabolism, maintain normal cell function, and indirectly provide stable physiological conditions for the generation of sperm. Cordyceps militaris polysaccharide has significant immunomodulation and antioxidant capacity, not only helps to reduce the free radical damage in the body, but also enhances the overall resistance of the body, can prevent the invasion of foreign pathogens to the reproductive system, and ensure that sperm matures in the best state. When cordycepin, adenosine and Cordyceps militaris polysaccharide three act synergistically, they jointly create a microenvironment that is conducive to male reproductive health, can effectively improve testosterone levels, improve sperm density, effectively reduce deformity rate, thereby improving sperm quality. At the same time, it is combined with Cistanche, Cynomorium songaricum, Cuscuta australis, Dipsacus asper, and Eucommia ulmoides to enhance the effects of strengthening yang and tonifying the kidney, and treating premature ejaculation and impotence. Morels, shiitake mushrooms, wolfberries, Schisandra chinensis, and Poria are used to correct the flavor. It also has the effects of tonifying the kidney and calming the nerves. Through the reasonable ratio of the raw materials, the biological activity and the medicinal and edible characteristics of each ingredient are fully exerted. The synergistic interaction between the functions of multiple drugs is stronger than the additive effect of a single drug. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 A pharmaceutical preparation in the form of a tablet prepared according to the present invention;

[0019] Figure 2 A pharmaceutical preparation in powder form prepared by the present invention;

[0020] Figure 3 A pharmaceutical preparation in the form of a pill prepared by the present invention;

[0021] Figure 4 A pharmaceutical preparation in the form of an oral solution prepared by the present invention;

[0022] Figure 5 The sperm morphology of mice in different groups in the experiment of the present invention is shown in FIG. DETAILED DESCRIPTION

[0023] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0024] Example 1: A cordycepin composition for improving sperm quality

[0025] Preparation of tablet composition

[0026] Formula: 1 part of Cordyceps militaris extract, 1 part of Morchella, 15 parts of Lentinus edodes, 15 parts of Cistanche deserticola, 1 part of Cynomorium songaricum, 10 parts of Lycium barbarum, 10 parts of Schisandra chinensis, 4 parts of Cuscuta australis, 5 parts of Dipsacus asper, 3 parts of Eucommia ulmoides, 1 part of Poria cocos and 18 parts of edible auxiliary materials.

[0027] In the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is 58 wt %, the content of adenosine is 10 wt %, and the content of Cordyceps militaris polysaccharide is 22 wt %.

[0028] Tablet preparation: Weigh the raw materials according to the above formula, mix them evenly, and make them into Figure 1 Tablets as indicated.

[0029] Example 2: A cordycepin composition for improving sperm quality

[0030] Preparation of tablet composition

[0031] Formula: 5 parts of Cordyceps militaris extract, 15 parts of Morchella, 1 part of Lentinus edodes, 1 part of Cistanche deserticola, 5 parts of Cynomorium songaricum, 8 parts of Lycium barbarum, 6 parts of Schisandra chinensis, 10 parts of Cuscuta australis, 3 parts of Dipsacus asper, 10 parts of Eucommia ulmoides, 3 parts of Poria cocos and 20 parts of edible auxiliary materials.

[0032] In the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is 50wt%, the content of adenosine is 10wt%, and the content of Cordyceps militaris polysaccharide is 30wt%.

[0033] Tablet preparation: Weigh the raw materials according to the above formula, mix them evenly and make tablets.

[0034] It should be noted that the extraction of cordycepin and adenosine in Examples 1 and 2 was performed by an assisted ultrasonic extraction method, specifically, the Cordyceps militaris powder was mixed with 30% ethanol at a solid-liquid ratio of 1:10 and placed in an ultrasonic extraction device, the temperature was set to 30°C and the frequency was 50kHz. After ultrasonic extraction for 90 minutes, the filtrate was filtered using a 0.45μm membrane, and dried using a rotary evaporator to obtain a sample.

[0035] The polysaccharide was extracted by enzymatic hydrolysis. Cordyceps powder was mixed with distilled water at a solid-liquid ratio of 1:10, and cellulase, pectinase and papain were added. After complete dissolution, the mixture was placed in a water bath at 50°C for 70 minutes. After the water bath, the mixture was heated to 90°C for 15 minutes to denature and inactivate the added enzyme. The supernatant was then collected by centrifugation at 6000 r / min for 10 minutes and concentrated using a rotary evaporator. After cooling to room temperature, 4 times the volume of 95% ethanol was added while stirring, and the mixture was centrifuged at 6000 r / min for 10 minutes to collect the supernatant. The protein was then removed by the Sevag method, and the solid was freeze-dried after 36 hours of dialysis to obtain polysaccharides.

[0036] Example 3: A cordycepin-containing composition for improving sperm quality

[0037] Preparation of powdered composition

[0038] Formula: 1 part of Cordyceps militaris extract, 1 part of Morchella, 15 parts of Lentinus edodes, 15 parts of Cistanche deserticola, 3 parts of Cynomorium songaricum, 1 part of Lycium barbarum, 4 parts of Schisandra chinensis, 3 parts of Cuscuta australis, 10 parts of Dipsacus asper, 1 part of Eucommia ulmoides, 10 parts of Poria cocos and 12 parts of edible auxiliary materials.

[0039] In the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is 50wt%, the content of adenosine is 20wt%, and the content of Cordyceps militaris polysaccharide is 20wt%.

[0040] Powder preparation: weigh the raw materials according to the above formula, mix them evenly, and make them into Figure 2 Powder as shown.

[0041] Example 4: A cordycepin-containing composition for improving sperm quality

[0042] Preparation of capsule composition

[0043] Formula: 1 part of Cordyceps militaris extract, 1 part of Morchella, 5 parts of Lentinus edodes, 4 parts of Cistanche deserticola, 4 parts of Cynomorium songaricum, 3 parts of Lycium barbarum, 1 part of Schisandra chinensis, 8 parts of Cuscuta australis, 1 part of Dipsacus asper, 2 parts of Eucommia ulmoides, 2 parts of Poria cocos and 11 parts of edible auxiliary materials.

[0044] In the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is 52wt%, the content of adenosine is 15wt%, and the content of Cordyceps militaris polysaccharide is 23wt%.

[0045] Capsule preparation: weigh the raw materials according to the above formula, mix them evenly and make hard capsules.

[0046] It should be noted that the extraction of cordycepin and adenosine in Examples 3 and 4 was performed by an assisted ultrasonic extraction method, specifically, the Cordyceps militaris powder was mixed with 80% ethanol at a solid-liquid ratio of 1:40 and placed in an ultrasonic extraction device, the temperature was set to 70°C and the frequency was 20 kHz. After ultrasonic extraction for 30 minutes, the filtrate was filtered using a 0.45 μm membrane, and dried using a rotary evaporator to obtain a sample.

[0047] The polysaccharide is extracted by enzymatic hydrolysis. Cordyceps powder is mixed with distilled water at a solid-liquid ratio of 1:30, and cellulase, pectinase and papain are added. After complete dissolution, it is water bathed at 60°C for 50 minutes. After the water bath is completed, the temperature is raised to 100°C and heated for 10 minutes to denature and inactivate the added enzyme; then, the supernatant is collected by centrifugation at a speed of 8000r / min for 5 minutes and concentrated using a rotary evaporator. After cooling to room temperature, 6 times the volume of 95% ethanol is added while stirring, and the supernatant is collected by centrifugation at a speed of 8000r / min for 5 minutes again. The protein is removed by the Sevag method, and the solid obtained after 48 hours of dialysis is freeze-dried to obtain polysaccharides.

[0048] Example 5: A cordycepin-containing composition for improving sperm quality

[0049] Preparation of pill composition

[0050] Formula: 5 parts of Cordyceps militaris extract, 3 parts of Morchella, 2 parts of Lentinus edodes, 4 parts of Cistanche deserticola, 5 parts of Cynomorium songaricum, 3 parts of Lycium barbarum, 1 part of Schisandra chinensis, 1 part of Cuscuta australis, 8 parts of Dipsacus asper, 7 parts of Eucommia ulmoides, 2 parts of Poria cocos and 15 parts of edible auxiliary materials.

[0051] In the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is 60wt%, the content of adenosine is 18wt%, and the content of Cordyceps militaris polysaccharide is 22wt%.

[0052] Pill preparation: weigh the raw materials according to the above formula, mix them evenly, and make them into Figure 3 Pills shown.

[0053] Example 6: A cordycepin-containing composition for improving sperm quality

[0054] Preparation of pill composition

[0055] Formula: 5 parts of Cordyceps militaris extract, 12 parts of Morchella, 12 parts of Lentinus edodes, 12 parts of Cistanche deserticola, 2 parts of Cynomorium songaricum, 4 parts of Lycium barbarum, 4 parts of Schisandra chinensis, 7 parts of Cuscuta australis, 6 parts of Dipsacus asper, 5 parts of Eucommia ulmoides, 6 parts of Poria cocos and 10 parts of edible auxiliary materials.

[0056] In the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is 50wt%, the content of adenosine is 10wt%, and the content of Cordyceps militaris polysaccharide is 30wt%.

[0057] Pill making: weigh the raw materials according to the above formula, mix them evenly and make them into pills.

[0058] Example 7: A cordycepin-containing composition for improving sperm quality

[0059] Preparation of oral solution composition

[0060] Formula: 2 parts of Cordyceps militaris extract, 4 parts of Morchella, 4 parts of Lentinus edodes, 6 parts of Cistanche deserticola, 2 parts of Cynomorium songaricum, 4 parts of Lycium barbarum, 4 parts of Schisandra chinensis, 4 parts of Cuscuta australis, 4 parts of Dipsacus asper, 8 parts of Eucommia ulmoides, 8 parts of Poria cocos and 10 parts of edible auxiliary materials.

[0061] In the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is 50wt%, the content of adenosine is 10wt%, and the content of Cordyceps militaris polysaccharide is 30wt%.

[0062] Preparation of oral solution: weigh the raw materials according to the above formula, mix them evenly, and prepare oral solution.

[0063] It should be noted that the extraction of cordycepin and adenosine in Examples 5-7 was carried out by an assisted ultrasonic extraction method, specifically: Cordyceps militaris powder was mixed with 40% ethanol at a solid-liquid ratio of 1:20 and placed in an ultrasonic extraction device, the temperature was set to 50°C and the frequency was 80kHz. After ultrasonic extraction for 60 minutes, the filtrate was filtered using a 0.45μm membrane, and dried using a rotary evaporator to obtain a sample.

[0064] The polysaccharide is extracted by enzymatic hydrolysis. Cordyceps powder is mixed with distilled water at a solid-liquid ratio of 1:20, and cellulase, pectinase and papain are added. After complete dissolution, it is water bathed at 55°C for 60 minutes. After the water bath is completed, the temperature is raised to 95°C and heated for 12 minutes to denature and inactivate the added enzyme; then, the supernatant is collected by centrifugation at 7000r / min for 8 minutes, and concentrated using a rotary evaporator. After cooling to room temperature, 5 times the volume of 95% ethanol is added while stirring, and the supernatant is collected by centrifugation at 7000r / min for 8 minutes again. The protein is removed by the Sevag method, and the solid obtained after 42 hours of dialysis is freeze-dried to obtain polysaccharides.

[0065] Example 8: A cordycepin-containing composition for improving sperm quality

[0066] Preparation of oral solution composition

[0067] Formula: 2 parts of Cordyceps militaris extract, 4 parts of Morchella, 4 parts of Lentinus edodes, 6 parts of Cistanche deserticola, 2 parts of Cynomorium songaricum, 4 parts of Lycium barbarum, 4 parts of Schisandra chinensis, 4 parts of Cuscuta australis, 4 parts of Dipsacus asper, 8 parts of Eucommia ulmoides and 8 parts of Poria cocos.

[0068] In the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is 60wt%, the content of adenosine is 10wt%, and the content of Cordyceps militaris polysaccharide is 20wt%.

[0069] Preparation of oral solution: weigh the raw materials according to the above formula, mix them evenly, and prepare oral solution.

[0070] Example 9: A cordycepin-containing composition for improving sperm quality

[0071] Preparation of oral solution composition

[0072] Formula: 3 parts of Cordyceps militaris extract, 7 parts of Morchella, 7 parts of Lentinus edodes, 9 parts of Cistanche deserticola, 2 parts of Cynomorium songaricum, 6 parts of Lycium barbarum, 6 parts of Schisandra chinensis, 4 parts of Cuscuta australis, 4 parts of Dipsacus asper, 4 parts of Eucommia ulmoides, 4 parts of Poria cocos and 11 parts of edible auxiliary materials.

[0073] In the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is 50wt%, the content of adenosine is 20wt%, and the content of Cordyceps militaris polysaccharide is 20wt%.

[0074] Preparation of oral solution: weigh the raw materials according to the above formula, mix them evenly, and prepare oral solution.

[0075] It should be noted that the extraction of cordycepin and adenosine in Examples 8 and 9 adopts an assisted ultrasonic extraction method, specifically: the Cordyceps militaris powder is mixed with 60% ethanol at a solid-liquid ratio of 1:15 and placed in an ultrasonic extraction device, the temperature is set to 60°C and the frequency is 40kHz. After ultrasonic extraction for 40 minutes, the filtrate is filtered using a 0.45μm membrane, and dried using a rotary evaporator to obtain a sample.

[0076] The polysaccharide was extracted by enzymatic hydrolysis. Cordyceps militaris powder was mixed with distilled water at a solid-liquid ratio of 1:15, and cellulase, pectinase and papain were added. After complete dissolution, the mixture was placed in a water bath at 58°C for 55 minutes. After the water bath, the mixture was heated to 92°C for 14 minutes to denature and inactivate the added enzyme. The mixture was then centrifuged at 6500 r / min for 6 minutes to collect the supernatant, which was concentrated using a rotary evaporator. After cooling to room temperature, 4.5 times the volume of 95% ethanol was added while stirring, and the mixture was centrifuged at 6500 r / min for 6 minutes to collect the supernatant. The protein was then removed by the Sevag method, and the solid was freeze-dried after 45 hours of dialysis to obtain polysaccharides.

[0077] Example 10: A cordycepin-containing composition for improving sperm quality

[0078] Preparation of oral solution composition

[0079] Formula: 3 parts of Cordyceps militaris extract, 7 parts of Morchella, 7 parts of Lentinus edodes, 9 parts of Cistanche deserticola, 2 parts of Cynomorium songaricum, 6 parts of Lycium barbarum, 6 parts of Schisandra chinensis, 4 parts of Cuscuta australis, 4 parts of Dipsacus asper, 4 parts of Eucommia ulmoides, 4 parts of Poria cocos and 11 parts of edible auxiliary materials.

[0080] In the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is 60wt%, the content of adenosine is 10wt%, and the content of Cordyceps militaris polysaccharide is 20wt%.

[0081] Preparation of oral solution: weigh the raw materials according to the above formula, mix them evenly, and prepare oral solution.

[0082] Example 11: A cordycepin-containing composition for improving sperm quality

[0083] Preparation of oral solution composition

[0084] Formula: 4 parts of Cordyceps militaris extract, 9 parts of Morchella, 9 parts of Lentinus edodes, 12 parts of Cistanche deserticola, 2 parts of Cynomorium songaricum, 8 parts of Lycium barbarum, 8 parts of Schisandra chinensis, 4 parts of Cuscuta australis, 4 parts of Dipsacus asper, 4 parts of Eucommia ulmoides, 4 parts of Poria cocos and 12 parts of edible auxiliary materials.

[0085] In the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is 60wt%, the content of adenosine is 10wt%, and the content of Cordyceps militaris polysaccharide is 20wt%.

[0086] Preparation of oral solution: weigh the raw materials according to the above formula, mix them evenly, and prepare Figure 4 Oral solution as indicated.

[0087] It should be noted that the extraction of cordycepin and adenosine in Example 10 and Example 11 adopts an assisted ultrasonic extraction method, specifically: Cordyceps militaris powder is mixed with 50% ethanol at a solid-liquid ratio of 1:30 and placed in an ultrasonic extraction equipment, the temperature is set to 55°C and the frequency is 60kHz. After ultrasonic extraction for 50 minutes, the filtrate is filtered using a 0.45μm membrane, and dried using a rotary evaporator to obtain a sample.

[0088] The polysaccharide is extracted by enzymatic hydrolysis. Cordyceps powder is mixed with distilled water at a solid-liquid ratio of 1:25, and cellulase, pectinase and papain are added. After complete dissolution, it is water bathed at 52°C for 65 minutes. After the water bath is completed, the temperature is raised to 98°C and heated for 13 minutes to denature and inactivate the added enzyme; then, the supernatant is collected by centrifugation at 7500r / min for 7 minutes and concentrated using a rotary evaporator. After cooling to room temperature, 5.5 times the volume of 95% ethanol is added while stirring, and the supernatant is collected by centrifugation at 7500r / min for 7 minutes again. The protein is removed by the Sevag method, and the solid obtained after 40 hours of dialysis is freeze-dried to obtain polysaccharides.

[0089] Experimental part:

[0090] Bisphenol A is an organic synthetic monomer, mainly used as a chemical raw material for producing plastics. It is usually used to manufacture packaging materials, daily necessities, medical and other fields. According to literature reports, more than 100 tons of bisphenol A are released into the atmosphere every year, and can remain in the air, water, soil, buildings, food, paper and other objects. The human body is mainly exposed to it through eating foods containing bisphenol A, inhaling air and skin contact. It can damage the blood-testis barrier and interfere with sperm production, thereby leading to poor development of male spermatogenesis and causing male infertility.

[0091] A total of 80 male KM mice were randomly divided into two groups: a normal group: mice raised under normal conditions; and a damaged group: mice whose reproductive function was impaired by the use of bisphenol A.

[0092] The oral solutions prepared in Examples 7-11 of the present invention were used to treat the injured mice, and the sperm density and deformity rate of the mice damaged by bisphenol A were detected to evaluate the effect of the oral solutions of the present invention on the reproductive-related indices of mice.

[0093] Group A: under the premise of using bisphenol A to damage the reproductive function of mice, the mice were treated with Example 7; Group B: under the premise of using bisphenol A to damage the reproductive function of mice, the mice were treated with Example 8; Group C: under the premise of using bisphenol A to damage the reproductive function of mice, the mice were treated with Example 9; Group D: under the premise of using bisphenol A to damage the reproductive function of mice, the mice were treated with Example 10; Group E: under the premise of using bisphenol A to damage the reproductive function of mice, the mice were treated with Example 11.

[0094] A control group F was set up: on the premise of using bisphenol A to damage the reproductive function of mice, the mice were treated with an oral solution prepared from Wuzi Yanzong Pills available on the market. The specific administration method is shown in Table 1 below.

[0095] Table 1

[0096] Group Subcutaneous injection Oral gavage Normal group Cooking Oil Normal saline Injury Group Bisphenol A Normal saline A Bisphenol A Example 7 Oral solution prepared B Bisphenol A Example 8 Oral solution prepared C Bisphenol A Example 9 Oral solution prepared D Bisphenol A Example 10 Oral solution prepared E Bisphenol A Example 11 Oral solution prepared F Bisphenol A Oral solution prepared from commercially available Wuzi Yanzong Pills

[0097] The administration volume for each mouse was set at 0.25 mL, and the administration was continued for 30 days. One hour after the last administration, the animals were killed, and the testicles and epididymis were removed for detection. The experimental results of each group were averaged. The effects of each group on the sperm density and deformity rate of mice damaged by bisphenol A are shown in Table 2.

[0098] Table 2

[0099]

[0100]

[0101] As can be seen from Table 2, the comparison between the normal group and the damaged group proves that bisphenol A can cause reproductive damage to mice, resulting in decreased sperm quality. The comparison between groups A, B, C, D, E, and F and the damaged group shows that the oral solution prepared by Examples 7-11 and the commercially available Wu Zi Yan Zong Wan have different degrees of therapeutic effects. After continuous subcutaneous injection of bisphenol A, the sperm density of mice decreased and the deformity rate increased. After continuous use of the oral solution containing cordycepin, compared with the damaged group, the sperm density and deformity rate in groups A, B, C, D, and E were improved, and the improvement effect was better than the oral solution prepared by Wu Zi Yan Zong Wan in the control group F. Compared with group A, the cordycepin content in group B is higher, but the content of Cordyceps militaris polysaccharide is lower. It can be seen from Table 2 that the sperm density and deformity rate of mice in group B are better than those in group A. Similarly, compared with group C, the cordycepin content in group D increased, while the adenosine content decreased. It can also be seen from Table 2 that the sperm density and deformity rate of mice in group D were better than those in group C. Therefore, higher cordycepin content has a significant positive effect on improving sperm quality and reducing sperm deformity in mice; at the same time, lower Cordyceps militaris polysaccharide or adenosine content did not have a negative impact on sperm quality, but instead observed results that were better than those in control group F. In summary, the increase in cordycepin content is a key factor in improving the health of mouse sperm, indicating that increasing cordycepin content within a certain range can effectively promote the improvement of sperm quality.

[0102] Mouse sperm quality analysis

[0103] The epididymis of mice in the normal group, injury group, E group and F group were separated and placed in a glass container filled with saline. After removing excess tissue and fat, 1-2 mL of saline was added to an EP tube and incubated at 35-37°C for 15-30 minutes. Then, the sperm suspension was counted with eosin staining to observe the sperm morphology. Figure 5 As shown in the figure, it can be seen that compared with the injury group, the normal group has a significantly lower sperm density in the injury group, and there are deformed sperm such as headless sperm, tailless sperm, head deformity, and tail bend. Compared with the injury group, groups E and F have improved to varying degrees, but the sperm density in group E is significantly greater than that in group F, and the deformity rate is lower than that in group F.

[0104] The above detection method proves that the cordycepin-containing composition can significantly increase sperm density, effectively reduce the deformity rate, and thus improve sperm quality.

Claims

1. A cordycepin composition for improving sperm quality, characterized in that: The composition comprises the following components in parts by weight: 1-5 parts of Cordyceps militaris extract, 1-15 parts of Morchella, 1-15 parts of Lentinula edodes, 1-15 parts of Cistanche deserticola, 1-5 parts of Cynomorium songaricum, 1-10 parts of Lycium barbarum, 1-10 parts of Schisandra chinensis, 1-10 parts of Cuscuta australis, 1-10 parts of Dipsacus asper, 1-10 parts of Eucommia ulmoides and 1-10 parts of Poria cocos; In the Cordyceps militaris extract, based on the total dry weight of the extract in dry powder form, the content of cordycepin is in the range of 50wt% to 60wt%, the content of adenosine is in the range of 10wt% to 20wt%, and the content of Cordyceps militaris polysaccharide is in the range of 20wt% to 30wt%.

2. The composition according to claim 1, characterized in that The composition comprises the following components in parts by weight: 2-4 parts of Cordyceps militaris extract, 4-12 parts of Morchella, 4-12 parts of Lentinula edodes, 6-12 parts of Cistanche deserticola, 2-4 parts of Cynomorium songaricum, 4-8 parts of Lycium barbarum, 4-8 parts of Schisandra chinensis, 4-8 parts of Cuscuta australis, 4-8 parts of Dipsacus asper, 4-8 parts of Eucommia ulmoides and 4-8 parts of Poria cocos.

3. The composition according to any one of claims 1 to 2, characterized in that The composition also contains 10 to 20 portions of edible auxiliary materials.

4. The composition according to claim 1, characterized in that The extraction of cordycepin and adenosine adopts an assisted ultrasonic extraction method, specifically: Cordyceps militaris powder is mixed with ethanol with a concentration of 30-80% according to a solid-liquid ratio of 1: (10-40), and then placed in an ultrasonic extraction device, the temperature is set to 30-70°C and the frequency is set to 20-80kHz. After ultrasonic extraction for 30-90 minutes, a filtrate is obtained by suction filtration using a 0.45μm membrane, and the filtrate is dried using a rotary evaporator to obtain a sample.

5. The composition according to claim 4, characterized in that The extraction of cordycepin and adenosine adopts an assisted ultrasonic extraction method, specifically: Cordyceps militaris powder is mixed with 40-60% ethanol in a solid-liquid ratio of 1: (10-20) and placed in an ultrasonic extraction device, the temperature is set to 50-60° C. and the frequency is set to 40-60 kHz. After ultrasonic extraction for 30-60 minutes, a 0.45 μm membrane is used for filtration to obtain a filtrate, and the filtrate is dried using a rotary evaporator to obtain a sample.

6. The composition according to claim 1, characterized in that The polysaccharide is extracted by enzymatic hydrolysis, wherein Cordyceps militaris powder is mixed with distilled water at a solid-liquid ratio of 1:(10-30), cellulase, pectinase and papain are added, and after being completely dissolved, the mixture is water-bathed at 50-60°C for 50-70 minutes. After the water-bath is completed, the mixture is heated to 90-100°C for 10-15 minutes to denature and inactivate the added enzyme; the mixture is then centrifuged at a speed of 6000-8000 r / min for 5-10 minutes to collect the supernatant, and concentrated using a rotary evaporator; after cooling to room temperature, 4-6 times the volume of 95% ethanol is added while stirring, and the mixture is centrifuged at a speed of 6000-8000 r / min for 5-10 minutes to collect the supernatant, and the protein is removed by the Sevag method. After 36-48 hours of dialysis, the solid obtained is freeze-dried to obtain the polysaccharide.

7. A pharmaceutical preparation, characterized in that The traditional Chinese medicine preparation contains the composition according to any one of claims 1-6.

8. The pharmaceutical preparation according to claim 7, characterized in that The preparation is in the form of tablets, capsules, powders, pills or oral solutions.

9. Use of the composition according to any one of claims 1 to 6 or the pharmaceutical preparation according to any one of claims 7 to 8 in the preparation of a drug for improving sperm quality.

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