Compound whitening pill containing Chinese wolfberry fruit extract and preparation process of compound whitening pill

Abstract

The compound whitening pill is prepared from the following raw materials: lycium barbarum polysaccharide and lycium barbarum protein, fructus momordicae extract, roxburgh rose powder, acerola cherry powder, grape seed extract, globe artichoke extract, olive fruit powder, mulberry powder, blueberry powder, pomegranate concentrated powder, hydroxypropyl methyl cellulose, dextrin and sugar alcohol. Citric acid, white peach powder essence and a lubricant. The lycium barbarum extract polysaccharide and protein are used as main materials, the fructus momordicae extract and various natural plant extracts are used as auxiliary materials, and the raw materials are reasonably proportioned, so that the biological activity and medicinal and edible homology characteristics of the components are fully exerted, the activity of tyrosinase is remarkably inhibited, and the synthesis of collagen is promoted; therefore, the whitening effect is synergistically and complementarily enhanced, and aging is delayed. The main material of the plant whitening component adopted by the invention is derived from plant extracts, and the component is mild and safe, so that the pursuit of consumers on natural, healthy and green skin care concepts is met.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a composite whitening pill containing wolfberry extract and a preparation process thereof. Background Art

[0002] With the improvement of living standards, people are pursuing skin whitening more and more. There are many methods for skin whitening. On the one hand, whitening is carried out by chemical means, that is, choosing relevant whitening drugs on the market, but these drugs basically inhibit tyrosinase activity by adding chemical substances, thereby reducing the production of melanin. While producing whitening effects, they will cause irritation, damage and side effects to consumers' skin. On the other hand, whitening is carried out by physical means, that is, using relevant cosmetics to cover and brighten the skin, but this kind of cosmetics cannot fundamentally change the color and internal state of the skin, and may even accumulate on the surface of the skin, hindering the normal metabolism of skin tissue, and even causing skin allergies, pigmentation and other problems. Summary of the invention

[0003] The purpose of the present invention is to provide a composite whitening pill containing wolfberry extract and a preparation process thereof. The main ingredient of the plant whitening component selected by the present invention is mild and safe. The prepared whitening pill is natural, healthy, has no side effects, can effectively inhibit the synthesis of melanin, and achieve a whitening effect.

[0004] In order to achieve the above object, the present invention provides the following technical solutions:

[0005] A composite whitening pill containing wolfberry extract comprises the following raw materials in percentage by weight: 20%-35% of wolfberry polysaccharide+wort protein, 10%-20% of monk fruit extract, 1%-7.5% of roxburghii powder, 1%-10% of acerola powder, 0.6%-2% of grape seed extract, 0.5%-3% of artichoke extract, 1%-5.5% of olive fruit powder, 1%-8.5% of mulberry powder, 5%-15% of blueberry powder, 1%-9.3% of red pomegranate concentrated powder, 1-3% of hydroxypropyl methylcellulose, 10%-20% of dextrin, 9%-15% of sugar alcohol, 0.1%-0.8% of citric acid, 0.1%-0.6% of white peach powder flavor, and 0.5%-2% of lubricant.

[0006] Furthermore, the paste is selected from one of maltodextrin and B-cyclodextrin.

[0007] Furthermore, the sugar alcohol is selected from one of xylitol and maltitol.

[0008] Furthermore, the lubricant is magnesium stearate or calcium stearate.

[0009] Furthermore, the preparation method of wolfberry polysaccharide+wolfberry protein comprises the following steps:

[0010] (1) preparing materials: selecting dry and impurity-free wolfberries as raw materials and crushing them to obtain uniform wolfberry powder;

[0011] (2) hot water extraction: adding distilled water or ethanol to the wolfberry powder obtained in step (1) at a solid-liquid ratio of 1:(10-15) for extraction, stirring and extracting in a water bath at 70-80° C. for 2-3 hours to obtain a protein and polysaccharide extract;

[0012] (3) centrifugal separation: after cooling the protein and polysaccharide extract obtained in step (2) to room temperature, centrifuge at a speed of 7000-8000 r / min for 10-15 minutes to obtain a supernatant;

[0013] (4) Concentration: The supernatant is concentrated under reduced pressure using a rotary evaporator at a temperature of 50-60° C. to 1 / 5-1 / 4 of the original volume;

[0014] (5) Drying: The concentrated solution is transferred to a freeze dryer for freeze drying to obtain a mixture powder of wolfberry polysaccharide + wolfberry protein.

[0015] The present invention also provides a preparation process of a composite whitening pill containing wolfberry extract, comprising the following steps:

[0016] (1) Sieving: Use an 80-mesh sieve to sieve the sugar alcohol and white peach powder essence separately for later use;

[0017] (2) preparing materials: weighing the sugar alcohol, white peach powder essence and other raw materials sieved in step (1) in sequence according to the recipe;

[0018] (3) Mixing: uniformly mixing the other raw materials weighed in step (2) except the lubricant to obtain a granulation raw material;

[0019] (4) Granulation: Add 5% by weight of ethanol to the granulation material obtained in step (3), fully moisten and mix, and then granulate through a 16-mesh sieve to obtain a granular product;

[0020] (5) Drying: The formed particles obtained after step (4) are placed in an oven for drying;

[0021] (6) secondary mixing: passing the dried granules obtained in step (5) through a 16-mesh sieve to obtain dried granules, and then adding a lubricant and mixing evenly to obtain a raw material for plastic pellets;

[0022] (7) Pill molding: The raw material for pill molding obtained after step (6) is fed into a tablet press for pill molding to obtain finished whitening pills.

[0023] Furthermore, the concentration of ethanol in step (4) is 60%-75%.

[0024] Furthermore, the setting temperature of the oven in step (5) is 50-55°C.

[0025] Furthermore, the pressure of the tablet press in step (7) is 60-85 KN.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The present invention uses wolfberry extract polysaccharide and protein as main ingredients, and uses monk fruit extract and a variety of natural plant extracts as auxiliary materials. Through the reasonable proportion of each raw material, the biological activity and medicinal and edible homology of each component are fully exerted, tyrosinase activity is significantly inhibited and collagen synthesis is promoted, thereby synergistically complementing and enhancing whitening effects and delaying aging. The main ingredient of the plant whitening component used in the present invention is derived from plant extracts, and the ingredients are mild and safe, which meets consumers' pursuit of natural, healthy and green skin care concepts.

[0028] The present invention prepares and separates the Lycium barbarum polysaccharide and protein mixture from Lycium barbarum, and has significant biological activity. On the one hand, it can inhibit the activity of tyrosinase, thereby reducing the generation of melanin. On the other hand, the components such as polysaccharides and proteins in Lycium barbarum can also promote the synthesis and repair of collagen. Collagen is an important structural component of tissues such as skin, bones, and joints. Its synthesis and repair ability directly affect the health and function of tissues. Lycium barbarum polysaccharide and protein mixture promote the synthesis of collagen, which helps to maintain the elasticity and gloss of the skin, and prevents aging phenomena such as wrinkles and relaxation. Therefore, Lycium barbarum extract can play the role of whitening and nourishing the skin, improving uneven skin color, etc. Momordica grosvenori is also a treasure for both medicinal and edible purposes. Its extract contains rich biologically active ingredients, has the effects of moisturizing the throat, moistening the intestines and laxative, regulating the intestines, etc., and with this health care effect, the skin can be indirectly glossy from the inside out, and can be synergistic with Lycium barbarum polysaccharide and protein mixture at the same time, strengthen the antioxidant barrier of the skin, play a certain whitening effect, and have certain benefits to human health.

[0029] The invention selects a variety of natural plant extracts as auxiliary materials, wherein roxburghii powder and acerola cherry powder are rich in antioxidant substances such as vitamin C, can effectively inhibit the activity of tyrosinase, reduce the generation of melanin, and long-term use is helpful to brighten the skin color; grape seed extract, mulberry powder and blueberry powder are rich in anthocyanins, have strong antioxidant properties, can remove free radicals in the body, and thus reduce damage to the skin; and artichoke extract, olive fruit powder, blueberry powder and pomegranate concentrated powder also have a variety of antioxidant components, can inhibit the expression of tyrosinase, and hinder the synthesis of melanin. Therefore, these auxiliary materials cooperate with each other, can play a role in multiple aspects, and synergistically achieve whitening effects.

[0030] The present invention adopts sugar alcohol and dextrin as fillers and adhesives, which can increase the volume of the whitening pills and play a role in moisture absorption and moisturizing. Hydroxypropyl methylcellulose is selected as the adhesive to ensure the molding and stability of the whitening pills. At the same time, under the lubricating and flow-aiding effects of the lubricant, material adhesion can be reduced. These auxiliary materials cooperate with each other to jointly improve the quality of the whitening pills. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 The figures are the results of determination of the inhibition rate of tyrosinase activity of the whitening pills prepared by the control group and different embodiments of the present invention. DETAILED DESCRIPTION

[0032] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0033] Embodiment 1:

[0034] The present embodiment provides a composite whitening pill containing wolfberry extract, comprising the following raw materials in weight percentage: 35% wolfberry polysaccharide + wolfberry protein, 10% monk fruit extract, 5% roxburgh powder, 4% acerola powder, 0.6% grape seed extract, 1% artichoke extract, 2.5% olive fruit powder, 2.5% mulberry powder, 9.5% blueberry powder, 5.5% pomegranate concentrated powder, 3% hydroxypropyl methylcellulose, 10% maltodextrin, 9.2% xylitol, 0.6% citric acid, 0.4% white peach powder flavor, and 1.2% magnesium stearate.

[0035] The preparation process steps of this embodiment are as follows:

[0036] (1) Sieving: Use an 80-mesh sieve to sieve xylitol and white peach powder essence separately for later use;

[0037] (2) preparing materials: weighing the xylitol, white peach powder essence and other raw materials sieved in step (1) in sequence according to the recipe;

[0038] (3) Mixing: uniformly mixing the other raw materials weighed in step (2) except magnesium stearate to obtain a granulation raw material;

[0039] (4) Granulation: Add 60% ethanol at a concentration of 5% by weight to the granulation material obtained in step (3), fully moisten and mix, and then granulate through a 16-mesh sieve to obtain a granular product;

[0040] (5) Drying: The formed particles obtained after step (4) are placed in an oven and dried at a temperature of 50° C.;

[0041] (6) secondary mixing: passing the dried granules obtained in step (5) through a 16-mesh sieve to obtain dried granules, and then adding magnesium stearate and mixing evenly to obtain a raw material for plastic pellets;

[0042] (7) Pill molding: The raw material for the pills obtained after step (6) is fed into a tablet press, and is pressed into pills under a pressure of 75 kN to obtain finished whitening pills.

[0043] It should be noted that the steps for preparing Lycium barbarum polysaccharide + Lycium barbarum protein in this embodiment are as follows:

[0044] (1) preparing materials: selecting dry and impurity-free wolfberries as raw materials and crushing them to obtain uniform wolfberry powder;

[0045] (2) hot water extraction: adding distilled water or ethanol to the wolfberry powder obtained in step (1) at a solid-liquid ratio of 1:10 for extraction, stirring and extracting in a 70° C. water bath for 3 hours to obtain a protein and polysaccharide extract;

[0046] (3) Centrifugal separation: After the protein and polysaccharide extract obtained in step (2) is cooled to room temperature, centrifuge at a speed of 7000 r / min for 15 minutes to obtain a supernatant;

[0047] (4) Concentration: The supernatant was concentrated under reduced pressure using a rotary evaporator at a temperature of 50°C to 1 / 5 of the original volume;

[0048] (5) Drying: The concentrated solution is transferred to a freeze dryer for freeze drying to obtain a mixture powder of wolfberry polysaccharide + wolfberry protein.

[0049] Embodiment 2:

[0050] The present embodiment provides a composite whitening pill containing wolfberry extract, comprising the following raw materials in weight percentage: 31% wolfberry polysaccharide + wolfberry protein, 14% monk fruit extract, 5% roxburgh powder, 4% acerola powder, 0.6% grape seed extract, 1% artichoke extract, 2.5% olive fruit powder, 2.5% mulberry powder, 9.5% blueberry powder, 5.5% pomegranate concentrated powder, 1.5% hydroxypropyl methylcellulose, 12% B cyclodextrin, 9.2% maltitol, 0.6% citric acid, 0.4% white peach powder flavor, and 0.7% magnesium stearate.

[0051] The preparation process steps of this embodiment are as follows:

[0052] (1) Sieving: Use an 80-mesh sieve to sieve the maltitol and white peach powder flavor separately for later use;

[0053] (2) preparing materials: weighing the maltitol, white peach powder essence and other raw materials sieved in step (1) in sequence according to the recipe;

[0054] (3) Mixing: uniformly mixing the other raw materials weighed in step (2) except magnesium stearate to obtain a granulation raw material;

[0055] (4) Granulation: Add 5% by weight of 70% ethanol to the granulation material obtained in step (3), fully moisten and mix, and then granulate through a 16-mesh sieve to obtain a granular product;

[0056] (5) Drying: The formed particles obtained after step (4) are placed in an oven and dried at a temperature of 50° C.;

[0057] (6) secondary mixing: passing the dried granules obtained in step (5) through a 16-mesh sieve to obtain dried granules, and then adding magnesium stearate and mixing evenly to obtain a raw material for plastic pellets;

[0058] (7) Pill molding: The raw material for the pills obtained after step (6) is fed into a tablet press, and is pressed into pills under a pressure of 60 kN to obtain finished whitening pills.

[0059] It should be noted that the steps for preparing Lycium barbarum polysaccharide + Lycium barbarum protein in this embodiment are as follows:

[0060] (1) preparing materials: selecting dry and impurity-free wolfberries as raw materials and crushing them to obtain uniform wolfberry powder;

[0061] (2) hot water extraction: adding distilled water or ethanol to the wolfberry powder obtained in step (1) at a solid-liquid ratio of 1:15 for extraction, stirring and extracting in a water bath at 80° C. for 2 hours to obtain a protein and polysaccharide extract;

[0062] (3) Centrifugal separation: After the protein and polysaccharide extract obtained in step (2) is cooled to room temperature, centrifuge at a speed of 8000 r / min for 10 minutes to obtain a supernatant;

[0063] (4) Concentration: The supernatant was concentrated under reduced pressure using a rotary evaporator at 60°C to 1 / 4 of the original volume;

[0064] (5) Drying: The concentrated solution is transferred to a freeze dryer for freeze drying to obtain a mixture powder of wolfberry polysaccharide + wolfberry protein.

[0065] Embodiment 3:

[0066] The present embodiment provides a composite whitening pill containing wolfberry extract, comprising the following raw materials in weight percentage: 30% wolfberry polysaccharide + wolfberry protein, 20% monk fruit extract, 5% roxburgh powder, 4% acerola powder, 0.6% grape seed extract, 1% artichoke extract, 2.5% olive fruit powder, 2.5% mulberry powder, 6.5% blueberry powder, 5.5% pomegranate concentrated powder, 1.5% hydroxypropyl methylcellulose, 10% maltodextrin, 9.2% maltitol, 0.6% citric acid, 0.4% white peach powder flavor, and 0.7% magnesium stearate.

[0067] The preparation process steps of this embodiment are as follows:

[0068] (1) Sieving: Use an 80-mesh sieve to sieve the maltitol and white peach powder flavor separately for later use;

[0069] (2) preparing materials: weighing the maltitol, white peach powder essence and other raw materials sieved in step (1) in sequence according to the recipe;

[0070] (3) Mixing: uniformly mixing the other raw materials weighed in step (2) except magnesium stearate to obtain a granulation raw material;

[0071] (4) Granulation: Add 5% by weight of 70% ethanol to the granulation material obtained in step (3), fully moisten and mix, and then granulate through a 16-mesh sieve to obtain a granular product;

[0072] (5) Drying: The formed particles obtained after step (4) are placed in an oven and dried at a temperature of 52° C.;

[0073] (6) secondary mixing: passing the dried granules obtained in step (5) through a 16-mesh sieve to obtain dried granules, and then adding magnesium stearate and mixing evenly to obtain a raw material for plastic pellets;

[0074] (7) Pill molding: The raw material for the pills obtained after step (6) is fed into a tablet press, and is pressed into pills under a pressure of 65 kN to obtain finished whitening pills.

[0075] It should be noted that the steps for preparing Lycium barbarum polysaccharide + Lycium barbarum protein in this embodiment are as follows:

[0076] (1) preparing materials: selecting dry and impurity-free wolfberries as raw materials and crushing them to obtain uniform wolfberry powder;

[0077] (2) hot water extraction: adding distilled water or ethanol to the wolfberry powder obtained in step (1) at a solid-liquid ratio of 1:12 for extraction, stirring and extracting in a water bath at 75° C. for 2.5 hours to obtain a protein and polysaccharide extract;

[0078] (3) Centrifugal separation: After the protein and polysaccharide extract obtained in step (2) is cooled to room temperature, centrifuge at a speed of 7500 r / min for 12 minutes to obtain a supernatant;

[0079] (4) Concentration: The supernatant was concentrated under reduced pressure using a rotary evaporator at a temperature of 55°C to 2 / 9 of the original volume;

[0080] (5) Drying: The concentrated solution is transferred to a freeze dryer for freeze drying to obtain a mixture powder of wolfberry polysaccharide + wolfberry protein.

[0081] Embodiment 4:

[0082] The present embodiment provides a composite whitening pill containing wolfberry extract, comprising the following raw materials in weight percentage: 25% wolfberry polysaccharide + wolfberry protein, 10% monk fruit extract, 7% roxburgh powder, 4% acerola powder, 0.6% grape seed extract, 1% artichoke extract, 2.5% olive fruit powder, 2.5% mulberry powder, 13.5% blueberry powder, 7.5% pomegranate concentrated powder, 3% hydroxypropyl methylcellulose, 10% B cyclodextrin, 11.2% xylitol, 0.6% citric acid, 0.4% white peach powder flavor, and 1.2% calcium stearate.

[0083] The preparation process steps of this embodiment are as follows:

[0084] (1) Sieving: Use an 80-mesh sieve to sieve xylitol and white peach powder essence separately for later use;

[0085] (2) preparing materials: weighing the xylitol, white peach powder essence and other raw materials sieved in step (1) in sequence according to the recipe;

[0086] (3) Mixing: uniformly mixing the other raw materials weighed in step (2) except calcium stearate to obtain a granulation raw material;

[0087] (4) Granulation: Add 5% by weight of 75% ethanol to the granulation material obtained in step (3), fully moisten and mix, and then granulate through a 16-mesh sieve to obtain a granular product;

[0088] (5) Drying: The formed particles obtained after step (4) are placed in an oven and dried at a temperature of 55° C.;

[0089] (6) secondary mixing: the dried granules obtained in step (5) are passed through a 16-mesh sieve to obtain dried granules, and calcium stearate is added and mixed evenly to obtain a raw material for plastic pellets;

[0090] (7) Pill molding: The raw material for the pills obtained after step (6) is fed into a tablet press, and is pressed into pills under a pressure of 85 kN to obtain finished whitening pills.

[0091] It should be noted that the steps for preparing Lycium barbarum polysaccharide + Lycium barbarum protein in this embodiment are as follows:

[0092] (1) preparing materials: selecting dry and impurity-free wolfberries as raw materials and crushing them to obtain uniform wolfberry powder;

[0093] (2) hot water extraction: adding distilled water or ethanol to the wolfberry powder obtained in step (1) at a solid-liquid ratio of 1:14 for extraction, stirring and extracting in a water bath at 70° C. for 3 hours to obtain a protein and polysaccharide extract;

[0094] (3) Centrifugal separation: After the protein and polysaccharide extract obtained in step (2) is cooled to room temperature, centrifuge at a speed of 7800 r / min for 15 minutes to obtain a supernatant;

[0095] (4) Concentration: The supernatant was concentrated under reduced pressure using a rotary evaporator at a temperature of 50° C. to 1 / 4 of the original volume;

[0096] (5) Drying: The concentrated solution is transferred to a freeze dryer for freeze drying to obtain a mixture powder of wolfberry polysaccharide + wolfberry protein.

[0097] Example 5

[0098] The present embodiment provides a composite whitening pill containing wolfberry extract, comprising the following raw materials in weight percentage: 22% wolfberry polysaccharide + wolfberry protein, 13% monk fruit extract, 7.5% roxburghii powder, 1% acerola powder, 1.6% grape seed extract, 3% artichoke extract, 5.5% olive fruit powder, 8.5% mulberry powder, 5% blueberry powder, 1% pomegranate concentrated powder, 1% hydroxypropyl methylcellulose, 20% B cyclodextrin, 9% xylitol, 0.8% citric acid, 0.6% white peach powder flavor, and 0.5% calcium stearate.

[0099] The preparation process steps of this embodiment are as follows:

[0100] (1) Sieving: Use an 80-mesh sieve to sieve xylitol and white peach powder essence separately for later use;

[0101] (2) preparing materials: weighing the xylitol, white peach powder essence and other raw materials sieved in step (1) in sequence according to the recipe;

[0102] (3) Mixing: uniformly mixing the other raw materials weighed in step (2) except calcium stearate to obtain a granulation raw material;

[0103] (4) Granulation: Add 60% ethanol at a concentration of 5% by weight to the granulation material obtained in step (3), fully moisten and mix, and then granulate through a 16-mesh sieve to obtain a granular product;

[0104] (5) Drying: The formed particles obtained after step (4) are placed in an oven and dried at a temperature of 50° C.;

[0105] (6) secondary mixing: the dried granules obtained in step (5) are passed through a 16-mesh sieve to obtain dried granules, and calcium stearate is added and mixed evenly to obtain a raw material for plastic pellets;

[0106] (7) Pill molding: The raw material for the pills obtained after step (6) is fed into a tablet press, and is pressed into pills under a pressure of 75 kN to obtain finished whitening pills.

[0107] It should be noted that the steps for preparing Lycium barbarum polysaccharide + Lycium barbarum protein in this embodiment are as follows:

[0108] (1) preparing materials: selecting dry and impurity-free wolfberries as raw materials and crushing them to obtain uniform wolfberry powder;

[0109] (2) hot water extraction: adding distilled water or ethanol to the wolfberry powder obtained in step (1) at a solid-liquid ratio of 1:10 for extraction, stirring and extracting in a 70° C. water bath for 3 hours to obtain a protein and polysaccharide extract;

[0110] (3) Centrifugal separation: After the protein and polysaccharide extract obtained in step (2) is cooled to room temperature, centrifuge at a speed of 7000 r / min for 15 minutes to obtain a supernatant;

[0111] (4) Concentration: The supernatant was concentrated under reduced pressure using a rotary evaporator at a temperature of 50°C to 1 / 5 of the original volume;

[0112] (5) Drying: The concentrated solution is transferred to a freeze dryer for freeze drying to obtain a mixture powder of wolfberry polysaccharide + wolfberry protein.

[0113] Example 6

[0114] The present embodiment provides a composite whitening pill containing wolfberry extract, comprising the following raw materials in weight percentage: wolfberry polysaccharide + wolfberry protein 20%, monk fruit extract 12%, roxburgh powder 1%, acerola powder 10%, grape seed extract 2%, artichoke extract 0.5%, olive fruit powder 1%, mulberry powder 1%, blueberry powder 15%, pomegranate concentrated powder 9.3%, hydroxypropyl methylcellulose 1%, B cyclodextrin 10%, xylitol 15%, citric acid 0.1%, white peach powder flavor 0.1%, and magnesium stearate 2%.

[0115] The preparation process steps of this embodiment are as follows:

[0116] (1) Sieving: Use an 80-mesh sieve to sieve xylitol and white peach powder essence separately for later use;

[0117] (2) preparing materials: weighing the xylitol, white peach powder essence and other raw materials sieved in step (1) in sequence according to the recipe;

[0118] (3) Mixing: uniformly mixing the other raw materials weighed in step (2) except magnesium stearate to obtain a granulation raw material;

[0119] (4) Granulation: Add 60% ethanol at a concentration of 5% by weight to the granulation material obtained in step (3), fully moisten and mix, and then granulate through a 16-mesh sieve to obtain a granular product;

[0120] (5) Drying: The formed particles obtained after step (4) are placed in an oven and dried at a temperature of 50° C.;

[0121] (6) secondary mixing: passing the dried granules obtained in step (5) through a 16-mesh sieve to obtain dried granules, and then adding magnesium stearate and mixing evenly to obtain a raw material for plastic pellets;

[0122] (7) Pill molding: The raw material for the pills obtained after step (6) is fed into a tablet press, and is pressed into pills under a pressure of 75 kN to obtain finished whitening pills.

[0123] It should be noted that the steps for preparing Lycium barbarum polysaccharide + Lycium barbarum protein in this embodiment are as follows:

[0124] (1) preparing materials: selecting dry and impurity-free wolfberries as raw materials and crushing them to obtain uniform wolfberry powder;

[0125] (2) hot water extraction: adding distilled water or ethanol to the wolfberry powder obtained in step (1) at a solid-liquid ratio of 1:14 for extraction, stirring and extracting in a water bath at 70° C. for 3 hours to obtain a protein and polysaccharide extract;

[0126] (3) Centrifugal separation: After the protein and polysaccharide extract obtained in step (2) is cooled to room temperature, centrifuge at a speed of 7800 r / min for 15 minutes to obtain a supernatant;

[0127] (4) Concentration: The supernatant was concentrated under reduced pressure using a rotary evaporator at a temperature of 50° C. to 1 / 4 of the original volume;

[0128] (5) Drying: The concentrated solution is transferred to a freeze dryer for freeze drying to obtain a mixture powder of wolfberry polysaccharide + wolfberry protein.

[0129] Experimental part: Tyrosinase inhibition test

[0130] Tyrosinase is a key enzyme in melanin synthesis, and its excessive activity can lead to skin pigmentation, thereby causing the formation of spots. Therefore, inhibiting the activity of tyrosinase can prevent and treat pigmentation and achieve a whitening effect. Although China's "Technical Specifications for Safety of Cosmetics" (GB 7916-2019) does not set specific standards for tyrosinase inhibition rate, it is generally believed in scientific research and the industry that when the tyrosinase inhibition rate of a certain raw material or product reaches more than 50%, the product is generally considered to have a good whitening effect. Therefore, the present invention evaluates the whitening effect of the whitening pill of the present invention by the inhibition rate of tyrosinase.

[0131] The whitening pills obtained in Examples 1-6 were fully crushed and dissolved in a PBS buffer solution with a pH of 6.8 to prepare six 1.0 mg / ml whitening pill sample solutions. The six whitening pill sample solutions were subjected to the following experiments: L-tyrosine solution, whitening pill sample solution, and PBS buffer were added to four groups of test tubes in sequence as shown in Table 1, and each test tube was preheated at 37°C. After 5 minutes of timing, 0.5 ml of tyrosinase solution with a specific activity of 100 U / ml was added in sequence and quickly mixed. After the system reacted for 10 minutes, the OD value was measured at 475 nm.

[0132] During the whole experiment, a control group was set up. Except for replacing the whitening pill solution with arbutin, the remaining operation steps of the control group were exactly the same as those of the whitening pill sample solutions of Examples 1-6. In addition, to ensure the accuracy of the experimental data, each group of experiments was measured in parallel 3 times.

[0133] Table 1

[0134]

[0135] According to the OD value measured each time, the formula: Inhibition rate (%) = [1-(T 3 -T 4 ) / (T 1 -T 2 )], calculate the inhibition rate of tyrosinase activity and plot it, as shown in Figure 1 The results of the calculated inhibition rate of tyrosinase activity are shown in Table 2.

[0136] Table 2 Tyrosinase activity inhibition rate test results

[0137] Group Tyrosinase inhibition (%) Control group (Arbutin) 73.2±0.98 Example 1 89.5±1.51 Example 2 84.9±1.38 Example 3 83.8±1.53 Example 4 82.5±0.87 Example 5 81.1±1.45 Example 6 80.1±0.95

[0138] From Table 2 and Figure 1 The results shown show that the whitening pills prepared by the present invention have an inhibition rate of 80.1%-89.5% in the tyrosinase activity inhibition test, and have a strong tyrosinase inhibition effect, which is better than the positive control arbutin. In addition, compared with the control group, the whitening pills of the present invention have significant differences. Therefore, the present invention combines wolfberry polysaccharide + wolfberry protein with natural plant extracts such as monk fruit, and the prepared whitening pills are natural and healthy, have no side effects, can effectively inhibit the synthesis of melanin, and achieve whitening effects.

[0139] In order to explore the synergistic effect of Momordica grosvenori extract on the whitening effect of Lycium barbarum polysaccharide + Lycium barbarum protein, Momordica grosvenori extract was removed on the basis of Examples 1-6, and other operations were kept unchanged to prepare comparative samples. The obtained tyrosinase inhibition rates were 85.3% ± 1.04, 81.4% ± 1.15, 80.2% ± 1.53, 79.2% ± 1.21, 78.8% ± 1.41, and 78.4% ± 1.37, respectively, which were significantly lower than the inhibition rates of tyrosinase activity of the whitening pills prepared in Examples 1-6 of the present invention, indicating that Momordica grosvenori extract has a good synergistic effect on the whitening effect of Lycium barbarum polysaccharide + Lycium barbarum protein. At the same time, the tyrosinase inhibition rates of Examples 2-6 of the present invention were significantly lower than those of Example 1, indicating that the synergistic effect was best when the ratio of Momordica grosvenori extract to Lycium barbarum polysaccharide + Lycium barbarum protein was 1:3.5, which could better promote skin metabolism, help collagen formation, and achieve whitening effect.

Claims

1. A composite whitening pill containing wolfberry extract, characterized in that: The invention comprises the following raw materials in weight percentage: 20%-35% of wolfberry polysaccharide + wolfberry protein, 10%-20% of monk fruit extract, 1%-7.5% of roxburghii powder, 1%-10% of acerola powder, 0.6%-2% of grape seed extract, 0.5%-3% of artichoke extract, 1%-5.5% of olive fruit powder, 1%-8.5% of mulberry powder, 5%-15% of blueberry powder, 1%-9.3% of pomegranate concentrated powder, 1-3% of hydroxypropyl methylcellulose, 10%-20% of dextrin, 9%-15% of sugar alcohol, 0.1%-0.8% of citric acid, 0.1%-0.6% of white peach powder flavor and 0.5%-2% of lubricant.

2. The composite whitening pill containing wolfberry extract according to claim 1, characterized in that: The paste is selected from one of maltodextrin or B-cyclodextrin.

3. The composite whitening pill containing wolfberry extract according to claim 1, characterized in that: The sugar alcohol is selected from one of xylitol and maltitol.

4. The composite whitening pill containing wolfberry extract according to claim 1, characterized in that: The lubricant is magnesium stearate or calcium stearate.

5. The composite whitening pill containing wolfberry extract according to claim 1, characterized in that: The preparation method of wolfberry polysaccharide+wolfberry protein comprises the following steps: (1) preparing materials: selecting dry and impurity-free wolfberries as raw materials and crushing them to obtain uniform wolfberry powder; (2) hot water extraction: adding distilled water or ethanol to the wolfberry powder obtained in step (1) at a solid-liquid ratio of 1:(10-15) for extraction, stirring and extracting in a water bath at 70-80° C. for 2-3 hours to obtain a protein and polysaccharide extract; (3) centrifugal separation: after cooling the protein and polysaccharide extract obtained in step (2) to room temperature, centrifuge at a speed of 7000-8000 r / min for 10-15 minutes to obtain a supernatant; (4) Concentration: The supernatant is concentrated under reduced pressure using a rotary evaporator at a temperature of 50-60° C. to 1 / 5-1 / 4 of the original volume; (5) Drying: The concentrated solution is transferred to a freeze dryer for freeze drying to obtain a mixture powder of wolfberry polysaccharide + wolfberry protein.

6. A process for preparing the composite whitening pill containing wolfberry extract according to any one of claims 1 to 5, characterized in that: The following steps are involved: (1) Sieving: Use an 80-mesh sieve to sieve the sugar alcohol and white peach powder essence separately for later use; (2) preparing materials: weighing the sugar alcohol, white peach powder essence and other raw materials sieved in step (1) in sequence according to the recipe; (3) Mixing: uniformly mixing the other raw materials weighed in step (2) except the lubricant to obtain a granulation raw material; (4) Granulation: Add 5% by weight of ethanol to the granulation material obtained in step (3), fully moisten and mix, and then granulate through a 16-mesh sieve to obtain a granular product; (5) Drying: The formed particles obtained after step (4) are placed in an oven for drying; (6) secondary mixing: passing the dried granules obtained in step (5) through a 16-mesh sieve to obtain dried granules, and then adding a lubricant and mixing evenly to obtain a raw material for plastic pellets; (7) Pill molding: The raw material for pill molding obtained after step (6) is fed into a tablet press for pill molding to obtain finished whitening pills.

7. The manufacturing process according to claim 6, characterized in that: The concentration of ethanol in step (4) is 60%-75%.

8. The manufacturing process according to claim 6, characterized in that: The setting temperature of the oven in step (5) is 50-55°C.

9. The manufacturing process according to claim 6, characterized in that: The pressure of the tablet press in step (7) is 60-85KN.

Images