Composite environmental stress control system for monitoring aquatic animal behaviors

Abstract

The invention belongs to the technical field of aquatic organism research, and particularly relates to a composite environmental stress control system for monitoring aquatic animal behaviors, which comprises a monitoring unit, a temperature control unit, a dissolved oxygen control unit and a carbon dioxide partial pressure control unit. The monitoring unit comprises a water basin, an inner separation cylinder and a camera. The inner separation cylinder divides the inner space of the water basin into an animal placing area and an equipment placing area. The water pumping end and the water return end of the temperature control unit, the nitrogen output end of the dissolved oxygen control unit and the mixed gas output end of the carbon dioxide partial pressure control unit are all located in the equipment containing area of the water basin. According to the device, the water body temperature, dissolved oxygen and carbon dioxide partial pressure can be accurately and rapidly adjusted in a laboratory environment, rapid disassembly and assembly can be achieved to adapt to multiple use scenes and different experiment scales, and the influence of related accessories of the device on normal physiological behaviors of the device can be effectively eliminated; therefore, the requirement of current behavioral research on a refined experiment platform is met.

Description

Technical Field

[0001] The invention belongs to the technical field of aquatic biological research, in particular to a composite environmental stress control system for monitoring the behavior of aquatic animals. Background Art

[0002] With the intensification of global climate change, the high temperature, low oxygen and acidification of the marine environment are becoming increasingly serious, greatly affecting the survival and behavior of aquatic organisms. Some benthic organisms and key aquatic animals in coastal ecosystems play an important role in maintaining the health and stability of the ecosystem. They are highly sensitive to environmental fluctuations and their growth, reproduction and behavior patterns will change significantly under stress. It is necessary to conduct single or combined stress experiments on aquatic animals with high temperature, low oxygen and acidification in a laboratory environment to clarify the effects of the above stress factors on their behavioral distribution, phenotype, physiology and biochemistry, and further explore their regulatory mechanisms.

[0003] Therefore, it is necessary to develop a control device for high temperature, low oxygen and acidification stress of aquatic animals, which can accurately and quickly adjust water temperature, dissolved oxygen and carbon dioxide partial pressure in a laboratory environment, realize comprehensive control of complex environmental stress conditions, and minimize the influence of device-related accessories on their normal physiological behavior and subsequent behavioral data analysis based on monitoring videos. Summary of the invention

[0004] In view of the above problems, an object of the present invention is to provide a composite environmental stress control system for monitoring the behavior of aquatic animals.

[0005] The objective of the present invention is achieved through the following technical solutions:

[0006] A composite environmental stress control system for monitoring aquatic animal behavior, comprising a monitoring unit, a temperature control unit, a dissolved oxygen control unit, and a carbon dioxide partial pressure control unit;

[0007] The monitoring unit includes a water basin, an inner partition, and a camera. The upper and lower ends of the inner partition are open and the inner partition is placed in the water basin. The inner partition divides the internal space of the water basin into an animal placement area and an equipment placement area. The space in the water basin and located on the inner side of the inner partition serves as the animal placement area. The annular space formed between the outer peripheral surface of the inner partition and the inner wall of the water basin serves as the equipment placement area. The inner partition is provided with a plurality of water passage holes respectively connected to the animal placement area and the equipment placement area. The camera is located above the water basin.

[0008] The temperature control unit has a water pumping end and a water return end, and the water pumping end and the water return end of the temperature control unit are respectively located in the equipment placement area of ​​the water basin. The temperature control unit is used to pump out the water in the water basin and heat or cool it down, and then transport the heated or cooled water back to the equipment placement area of ​​the water basin to achieve temperature regulation of the water in the water basin;

[0009] The dissolved oxygen control unit has a nitrogen output end, and the nitrogen output end of the dissolved oxygen control unit is located in the equipment placement area of ​​the water basin. The dissolved oxygen control unit is used to introduce nitrogen into the water in the water basin to control the dissolved oxygen concentration in the water;

[0010] The carbon dioxide partial pressure control unit has a mixed gas output end, which is located in the equipment placement area of ​​the water basin. The carbon dioxide partial pressure control unit is used to introduce a carbon dioxide-air mixed gas into the saturated water in the water basin to achieve acidification of the water body.

[0011] The temperature control unit includes a water pump A, a temperature control barrel, a heat exchange tube, a heating rod, a chiller, and a water pump B;

[0012] The chiller has a cold water output interface and a return water interface. The cold water output interface of the chiller is connected to one end of a cold water output pipeline, and the return water interface of the chiller is connected to one end of a cold water recovery pipeline. The other end of the cold water output pipeline is connected to the temperature control barrel, and the other end of the cold water recovery pipeline is connected to the output end of the water pump B. The water pump B, the heating rod, and the heat exchange tube are respectively arranged in the temperature control barrel. The heat exchange tube has a water input port and a water output port. The water input port of the heat exchange tube is connected to the output end of the water pump A through a water input pipeline, and the water output port of the heat exchange tube is connected to one end of the water output pipeline. The water pump A is located in the equipment placement area of ​​the water basin as the water pumping end of the temperature control unit, and the other end of the water output pipeline is located in the equipment placement area of ​​the water basin as the return water end of the temperature control unit.

[0013] The heat exchange tube is spiral and made of titanium tube; the heating rod is connected to a temperature controller located outside the temperature control barrel, the temperature controller is connected to a temperature probe, and the temperature probe is arranged in the temperature control barrel.

[0014] The dissolved oxygen control unit includes a nitrogen gas source, a dissolved oxygen meter, a solenoid valve, and a gas diffuser A;

[0015] The input end of the solenoid valve is connected to the nitrogen gas source, the output end of the solenoid valve is connected to the gas diffuser A through a pipeline, the dissolved oxygen meter is connected to the solenoid valve and controls the opening and closing of the solenoid valve, and the gas diffuser A is placed in the equipment placement area of ​​the water basin as the nitrogen output end of the dissolved oxygen control unit.

[0016] The dissolved oxygen meter is also connected to a dissolved oxygen sensor, and the dissolved oxygen sensor is located in the equipment placement area of ​​the water basin.

[0017] The carbon dioxide partial pressure control unit includes a carbon dioxide gas source, a pressure reducing valve, a carbon dioxide enricher, and a gas diffuser B;

[0018] The input end of the pressure reducing valve is connected to the carbon dioxide gas source, the carbon dioxide enricher has an air inlet, a carbon dioxide inlet interface and a mixed gas outlet interface, the air inlet of the carbon dioxide enricher is connected to the atmosphere, the carbon dioxide inlet interface of the carbon dioxide enricher is connected to the output end of the pressure reducing valve through a pipeline, the mixed gas outlet interface of the carbon dioxide enricher is connected to the gas diffuser B through a pipeline, and the gas diffuser B is placed in the equipment placement area of ​​the water basin as the mixed gas output end of the carbon dioxide partial pressure control unit.

[0019] The carbon dioxide enricher is also connected to a pH probe, which is located in the equipment placement area of ​​the water basin.

[0020] The camera is installed on a camera bracket, and a light source is also arranged on the camera bracket.

[0021] The bottom surface of the inner side of the water basin is white.

[0022] Each of the water body passage holes is located on the same side of the inner partition tube, and the water pumping end and water return end of the temperature control unit, the nitrogen output end of the dissolved oxygen control unit, and the mixed gas output end of the carbon dioxide partial pressure control unit are all located on the side of the equipment placement area of ​​the water basin away from each of the water body passage holes.

[0023] The advantages and positive effects of the present invention are:

[0024] The present invention can accurately and quickly adjust water temperature, dissolved oxygen and carbon dioxide partial pressure in a laboratory environment, realize comprehensive control of complex environmental stress conditions, facilitate single or complex stress experiments of high temperature, low oxygen and acidification on aquatic animals, have high equipment integration and control accuracy, can simultaneously monitor the behavior of aquatic animals in multiple water bodies, can achieve rapid disassembly and assembly to adapt to multiple usage scenarios and different experimental scales, and can effectively eliminate the influence of device-related accessories on its normal physiological behavior and subsequent behavioral data analysis based on monitoring videos, thereby meeting the current behavioral research needs for refined experimental platforms. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0026] Figure 2 It is a structural schematic diagram of the monitoring unit of the present invention;

[0027] Figure 3 It is a partial structural schematic diagram of the temperature control unit of the present invention when used to heat water in a basin;

[0028] Figure 4 It is a partial structural schematic diagram of the temperature control unit of the present invention when used to cool the water in the basin;

[0029] Figure 5 It is a structural schematic diagram of the dissolved oxygen control unit of the present invention;

[0030] Figure 6 It is a structural schematic diagram of the carbon dioxide partial pressure control unit of the present invention;

[0031] Figure 7 It is a schematic diagram of the top view of the monitoring unit of the present invention when in use;

[0032] Figure 8 This is a picture taken when the camera of the present invention is actually used.

[0033] In the figure: 1 is a temperature control barrel, 2 is a heat exchange tube, 3 is a water pump A, 4 is a heating rod, 5 is a temperature controller, 6 is a temperature probe, 7 is a water pump B, 8 is a chiller, 9 is a nitrogen gas source, 10 is a solenoid valve, 11 is a gas diffuser A, 12 is a dissolved oxygen meter, 13 is a dissolved oxygen sensor, 14 is a carbon dioxide enricher, 15 is a gas diffuser B, 16 is a pH probe, 17 is a pressure reducing valve, 18 is a carbon dioxide gas source, 19 is an air inlet, 20 is a light source, 21 is a camera, 22 is a camera bracket, 23 is an inner partition, 2301 is a water through hole, and 24 is a water basin; 001 is an animal placement area, and 002 is an equipment placement area. DETAILED DESCRIPTION

[0034] The following is combined with Figure 1-8 The present invention is further described in detail.

[0035] A composite environmental stress control system for monitoring the behavior of aquatic animals, such as Figure 1-8 As shown, this embodiment includes a monitoring unit, a temperature control unit, a dissolved oxygen control unit, and a carbon dioxide partial pressure control unit.

[0036] The monitoring unit includes a water basin 24, an inner partition 23, and a camera 21. The upper and lower ends of the inner partition 23 are open and the inner partition 23 is placed in the middle of the water basin 24. The inner partition 23 divides the internal space of the water basin 24 into an animal placement area 001 and an equipment placement area 002. The space in the water basin 24 and located on the inner side of the inner partition 23 serves as the animal placement area 001, and the annular space formed between the outer peripheral surface of the inner partition 23 and the inner wall of the water basin 24 serves as the equipment placement area 002. The inner partition 23 is provided with a plurality of water through holes 2301 respectively connected to the animal placement area 001 and the equipment placement area 002. The camera 21 is located above the water basin 24.

[0037] The temperature control unit has a pumping end and a return end, and the pumping end and the return end of the temperature control unit are respectively located in the equipment placement area 002 of the water basin 24. The temperature control unit is used to pump out the water in the water basin 24 and heat or cool it down, and then transport the heated or cooled water back to the equipment placement area 002 of the water basin 24 to achieve temperature regulation of the water in the water basin 24.

[0038] The dissolved oxygen control unit has a nitrogen output terminal, which is located in the equipment placement area 002 of the water basin 24. The dissolved oxygen control unit is used to introduce nitrogen into the water in the water basin 24 to control the dissolved oxygen concentration in the water.

[0039] The carbon dioxide partial pressure control unit has a mixed gas output end, which is located in the equipment placement area 002 of the water basin 24. The carbon dioxide partial pressure control unit is used to introduce a carbon dioxide-air mixed gas into the saturated water in the water basin 24 to achieve acidification of the water body.

[0040] When the experimental subjects are placed and monitored, the aquatic animals are placed in the experimental water body with adjusted environmental parameters, placed in the animal placement area 001 for temporary raising, and acclimated for a period of time. Make sure that only the corresponding aquatic animals are placed in the animal placement area 001. After the experiment starts, the camera 21 records the behavior of the experimental subjects. After the experiment is over, turn off the temperature control unit, dissolved oxygen control unit, and carbon dioxide partial pressure control unit, and move the experimental subjects out of the experimental water body. Disassemble and clean all equipment to ensure that the equipment is in good condition and ready for the next experiment. The system as a whole is easy to disassemble and assemble, and users can quickly clean and maintain it.

[0041] Each temperature control unit, dissolved oxygen control unit, and carbon dioxide partial pressure control unit can be used in conjunction with the water basins 24 of multiple monitoring units respectively, with high equipment integration and control accuracy. It can simultaneously monitor the behavior of aquatic animals in the water bodies corresponding to multiple monitoring units, and can be quickly disassembled and assembled to adapt to multiple usage scenarios and different experimental scales.

[0042] Specifically, if Figure 3 and Figure 4 As shown, the temperature control unit in this embodiment includes a water pump A 3, a temperature control barrel 1, a heat exchange tube 2, a heating rod 4, a chiller 8, and a water pump B 7.

[0043] The chiller 8 has a cold water output interface and a return water interface. The cold water output interface of the chiller 8 is connected to one end of the cold water output pipeline, and the return water interface of the chiller 8 is connected to one end of the cold water recovery pipeline. The other end of the cold water output pipeline is connected to the temperature control barrel 1, and the other end of the cold water recovery pipeline is connected to the output end of the water pump B7. The water pump B7, the heating rod 4, and the heat exchange tube 2 are respectively arranged in the temperature control barrel 1. The heat exchange tube 2 has a water input port and a water output port. The water input port of the heat exchange tube 2 is connected to the output end of the water pump A3 through the water input pipeline, and the water output port of the heat exchange tube 2 is connected to one end of the water output pipeline. The water pump A3 is located in the equipment placement area 002 of the water basin 24 as the water pumping end of the temperature control unit, and the other end of the water output pipeline is located in the equipment placement area 002 of the water basin 24 as the return water end of the temperature control unit. The water pump A3 delivers the water in the water basin 24 to the heat exchange tube 2, and exchanges heat with the water in the temperature control barrel 1, thereby heating or cooling the water in the water basin 24. The water temperature in the water basin 24 can be observed and obtained by real-time display through an external water thermometer.

[0044] In this embodiment, the temperature control barrel 1 adopts a 65L square plastic container; the water pump A3 and the water pump B7 are both commercially available submersible pump products; the heating rod 4 and the water chiller 8 are both commercially available products, and the water chiller 8 can adopt, for example, the water chiller product of the Aolin brand with a model of AL-SF102. In this embodiment, the heat exchange tube 2 is spiral and adopts a titanium tube, which effectively enhances the heat exchange effect. In this embodiment, the heating rod 4 is connected to the temperature controller 5 located outside the temperature control barrel 1, and the temperature controller 5 is connected to the temperature probe 6, and the temperature probe 6 is arranged in the temperature control barrel 1. The temperature controller 5 and the temperature probe 6 are both commercially available products. The temperature probe 6 is used to monitor the temperature of the water in the temperature control barrel 1 in real time; when heating, the measured temperature value can be fed back to the temperature controller 5 at the same time, and the temperature controller 5 controls the opening and closing of the heating rod 4; when cooling is required, the water chiller 8 and the water pump B7 are directly controlled to act, and the water pump B7 sends the water in the temperature control barrel 1 to the water chiller 8 for cooling. The water in the temperature control barrel 1 is adjusted to a desired temperature by heating with the heating rod 4 or cooling with the water chiller 8 .

[0045] Specifically, if Figure 5 As shown, the dissolved oxygen control unit in this embodiment includes a nitrogen gas source 9, a dissolved oxygen meter 12, a solenoid valve 10, and a gas diffuser A11.

[0046] The input end of the solenoid valve 10 is connected to the nitrogen gas source 9, and the output end of the solenoid valve 10 is connected to the gas diffuser A11 through a pipeline. The dissolved oxygen meter 12 is connected to the solenoid valve 10 and controls the opening and closing of the solenoid valve 10. The gas diffuser A11 is placed in the equipment placement area 002 of the water basin 24 as the nitrogen output end of the dissolved oxygen control unit.

[0047] In this embodiment, the nitrogen source 9 can be in other forms such as a nitrogen generator or a nitrogen cylinder. The nitrogen generator is a commercially available product, such as a nitrogen generator of model LCN-300 produced by Shanghai Lichen Instrument Technology Co., Ltd. In this embodiment, the dissolved oxygen meter 12 is a commercially available product, such as a dissolved oxygen meter product of model 6308DT produced by JENCO. The solenoid valve 10 is also a commercially available product, which is used to control the on-off of the nitrogen supply. In this embodiment, the gas diffuser A11 uses a commercially available gas stone or gas line product. In this embodiment, the dissolved oxygen meter 12 is also connected to a dissolved oxygen sensor 13, which is located in the equipment placement area 002 of the water basin 24. The dissolved oxygen sensor 13 is a commercially available product, and can monitor the dissolved oxygen concentration in the water basin 24 in real time by using a dissolved oxygen sensor product using optical sensing technology, and can transmit information to the dissolved oxygen meter 12. The dissolved oxygen meter 12 controls the opening and closing of the solenoid valve 10, and can achieve accurate control of the dissolved oxygen in the water body by introducing nitrogen into the water body to reduce the dissolved oxygen level. The number of gas diffusers A11 and dissolved oxygen sensors 13 can be adjusted arbitrarily according to the use requirements. Gas diffusers A11 and dissolved oxygen sensors 13 should be placed separately to avoid affecting the control accuracy due to the rapid reduction of dissolved oxygen in the local water body at the ventilation point. Set the high and low thresholds of dissolved oxygen in the dissolved oxygen meter 12. When the dissolved oxygen sensor 13 detects that the dissolved oxygen concentration in the water body is higher than the set "high point", the solenoid valve 10 is controlled to open, and nitrogen begins to enter the water body to reduce the dissolved oxygen; when the dissolved oxygen sensor 13 detects that the dissolved oxygen concentration drops to the set "low point", the solenoid valve 10 is controlled to close, stop the input of nitrogen, and restore the dissolved oxygen level of the water body.

[0048] Specifically, if Figure 6 As shown, the carbon dioxide partial pressure control unit in this embodiment includes a carbon dioxide gas source 18, a pressure reducing valve 17, a carbon dioxide enricher 14, and a gas diffuser B15.

[0049] The input end of the pressure reducing valve 17 is connected to the carbon dioxide gas source 18, the carbon dioxide enricher 14 has an air inlet 19, a carbon dioxide inlet interface and a mixed gas outlet interface, the air inlet 19 of the carbon dioxide enricher 14 is connected to the atmosphere, the carbon dioxide inlet interface of the carbon dioxide enricher 14 is connected to the output end of the pressure reducing valve 17 through a pipeline, the mixed gas outlet interface of the carbon dioxide enricher 14 is connected to the gas diffuser B15 through a pipeline, and the gas diffuser B15 is placed in the equipment placement area 002 of the water basin 24 as the mixed gas output end of the carbon dioxide partial pressure control unit.

[0050] In this embodiment, the carbon dioxide gas source 18 adopts a carbon dioxide gas cylinder, and other forms may also be adopted. The pressure reducing valve 17 adopts a commercially available manual pressure reducing valve product, which is convenient for adjusting the intake air pressure of the carbon dioxide enricher 14. In this embodiment, the carbon dioxide enricher 14 adopts a commercially available product, for example, a multi-channel carbon dioxide enricher product of model SFO-E06 produced by Starfish Instrument Company can be adopted. In this embodiment, the gas diffuser B15 also adopts a commercially available gas stone or gas line product, etc., and the number of gas diffusers B15 can be increased or decreased arbitrarily according to the use requirements. In this embodiment, the carbon dioxide enricher 14 is also connected to a pH probe 16, and the pH probe 16 is located in the equipment placement area 002 of the water basin 24. The pH probe 16 adopts a commercially available product for real-time monitoring of the pH of the water body. The carbon dioxide enricher 14 has a built-in sensor for monitoring the partial pressure of carbon dioxide in the output mixed gas, which can feedback information to the carbon dioxide enricher 14, so that the carbon dioxide enricher 14 adjusts the mixing ratio of carbon dioxide gas and air, and introduces the mixed gas into the water body, thereby adjusting the acidity of the water body.

[0051] Specifically, if Figure 1 , Figure 2 , Figure 7 and Figure 8 As shown, in this embodiment, the camera 21 is installed on the camera bracket 22, and the camera bracket 22 is also provided with a light source 20. The animal placement area 001 in the inner partition 23 is the observation area for video analysis, which can ensure that the video analysis window is free of debris and easy to analyze. The water basin 24 is used as a container for water and animals and a shooting background in the experiment. The bottom surface of the inner side of the water basin 24 is white, which can form a high contrast with the aquatic animals and is easy to analyze in the experiment; in this embodiment, the water basin 24 and the inner partition 23 are made entirely of white plastic materials. The light source 20 of this embodiment can use a commercially available soft light product to provide the light source required for video recording, ensure that the shooting picture is bright, and avoid the interference of light spots caused by water surface reflection. Because in the specific implementation, a relatively closed and light-shielded experimental environment is usually created, its lighting is completely dependent on the light source 20, the lighting is more controllable, and the shooting effect is better. In this embodiment, the camera 21 uses a commercially available product. When the aquatic animal moves slowly, the camera 21 preferably uses a commercially available time-lapse camera. The camera bracket 22 is provided with a structure that adopts the existing technology and can be provided with a structure that can adjust its own height, the shooting angle of the camera 21 and the irradiation angle of the light source 20, and is easy to use and adjust.

[0052] Each water body through hole 2301 is located on the same side of the inner partition 23, and the pumping end and the return end of the temperature control unit, the nitrogen output end of the dissolved oxygen control unit, and the mixed gas output end of the carbon dioxide partial pressure control unit are all located in the equipment placement area 002 of the water basin 24, on the side away from each water body through hole 2301. In this way, the specific air stone, water pump, sensor, etc. used can be located in the equipment placement area 002 and placed on the side without the water body through hole 2301, while the side with the water body through hole 2301 can achieve water exchange with the inside of the inner partition 23, which can effectively avoid the influence of the water flow generated by the air stone, water pump, etc. on aquatic animals, thereby meeting the current behavioral research needs for a refined experimental platform.

[0053] The composite environmental stress control system for monitoring aquatic animal behavior proposed in the present invention sets three temperature levels (16°C, 26°C, 32°C), three dissolved oxygen levels (2mg / L, 4mg / L, 7mg / L) and three carbon dioxide partial pressure levels (500ppm, 1000ppm, 1500ppm) in a specific experimental example, and a total of 9 groups of orthogonal experiments, A1, A2, A3, B1, B2, B3, C1, C2, C3, are designed. The specific configuration of each experimental group is as follows (expressed in terms of temperature-dissolved oxygen-carbon dioxide partial pressure):

[0054] 16℃-7mg / L-1500ppm;

[0055] 16℃-4mg / L-1000ppm;

[0056] 16℃-2mg / L-500ppm;

[0057] 26℃-7mg / L-500ppm;

[0058] 26℃-4mg / L-1500ppm;

[0059] 26℃-2mg / L-1000ppm;

[0060] 32℃-7mg / L-1000ppm;

[0061] 32℃-4mg / L-500ppm;

[0062] 32℃-2mg / L-1500ppm.

[0063] In this experimental example, the camera 21 uses a time-lapse photography device to record the movement behavior of the sea cucumber. During the experiment, the water quality parameters such as water temperature, dissolved oxygen, and pH value in the water basin 24 are monitored in real time. The relevant monitoring data and calculated values ​​are shown in Table 1 below, which are presented in the form of mean ± standard deviation.

[0064] Table 1 Water quality parameters (Mean±SD)

[0065]

[0066] The above experimental results show that under the control of this experimental system, water quality parameters can stably reach the set targets and maintain good control accuracy, which can meet the experimental requirements under the combined stress conditions of high temperature, low oxygen and acidification.

[0067] In the experiment, the motion images of sea cucumbers were taken (see Figure 8 , video screenshots) show good clarity, the picture is bright and without light spots, the analysis object is clearly contrasted with the background, and it is easy to identify. The picture background is neat and tidy, without foreign objects, and the motion data of sea cucumbers can be effectively identified and analyzed. In addition, the water surface in the inner diaphragm 23 in the experimental pool remains stable, without bubble and water flow interference, ensuring that the behavior of sea cucumbers during the experiment is not affected by external factors.

[0068] Through the composite environmental stress control system for monitoring aquatic animal behavior disclosed in the present invention, a high temperature, low oxygen, and acidification composite stress environment was successfully constructed, and the feasibility of the system was verified through experiments. The experiment shows that the experimental equipment and method used can effectively control water quality parameters and achieve the experimental goals. The motion images taken are clear and can provide reliable data support for subsequent aquatic animal behavior analysis.

Claims

1. A composite environmental stress control system for monitoring aquatic animal behavior, characterized in that: It includes a monitoring unit, a temperature control unit, a dissolved oxygen control unit, and a carbon dioxide partial pressure control unit; The monitoring unit comprises a water basin (24), an inner partition (23), and a camera (21). The upper and lower ends of the inner partition (23) are open. The inner partition (23) is placed in the water basin (24). The inner partition (23) divides the internal space of the water basin (24) into an animal placement area (001) and an equipment placement area (002). The space in the water basin (24) and located on the inner side of the inner partition (23) serves as the animal placement area (001). The annular space formed between the outer peripheral surface of the inner partition (23) and the inner wall of the water basin (24) serves as the equipment placement area (002). The inner partition (23) is provided with a plurality of water passage holes (2301) respectively connected to the animal placement area (001) and the equipment placement area (002). The camera (21) is located above the water basin (24). The temperature control unit has a water pumping end and a water return end, the water pumping end and the water return end of the temperature control unit are respectively located in the equipment placement area (002) of the water basin (24), and the temperature control unit is used to pump out the water in the water basin (24) and heat or cool it down, and then transport the heated or cooled water back to the equipment placement area (002) of the water basin (24) to achieve temperature regulation of the water in the water basin (24); The dissolved oxygen control unit has a nitrogen output end, and the nitrogen output end of the dissolved oxygen control unit is located in the equipment placement area (002) of the water basin (24). The dissolved oxygen control unit is used to introduce nitrogen into the water in the water basin (24) to control the dissolved oxygen concentration in the water; The carbon dioxide partial pressure control unit has a mixed gas output end, and the mixed gas output end of the carbon dioxide partial pressure control unit is located in the equipment placement area (002) of the water basin (24). The carbon dioxide partial pressure control unit is used to introduce carbon dioxide-air mixed gas into the saturated water in the water basin (24) to achieve acidification of the water body.

2. A composite environmental stress control system for monitoring aquatic animal behavior according to claim 1, characterized in that: The temperature control unit comprises a water pump A (3), a temperature control barrel (1), a heat exchange tube (2), a heating rod (4), a chiller (8), and a water pump B (7); The chiller (8) has a cold water output interface and a water return interface. The cold water output interface of the chiller (8) is connected to one end of a cold water output pipeline. The water return interface of the chiller (8) is connected to one end of a cold water recovery pipeline. The other end of the cold water output pipeline is connected to the temperature control barrel (1). The other end of the cold water recovery pipeline is connected to the output end of the water pump B (7). The water pump B (7), the heating rod (4), and the heat exchange tube (2) are respectively arranged in the temperature control barrel (1). The heat exchange tube (2) has a water input port and a water output port. The water input port of the heat exchange tube (2) is connected to the output end of the water pump A (3) through a water input pipeline. The water output port of the heat exchange tube (2) is connected to one end of the water output pipeline. The water pump A (3) is located in the equipment placement area (002) of the water basin (24) as a water pumping end of the temperature control unit. The other end of the water output pipeline is located in the equipment placement area (002) of the water basin (24) as a water return end of the temperature control unit.

3. A composite environmental stress control system for monitoring aquatic animal behavior according to claim 2, characterized in that: The heat exchange tube (2) is spiral-shaped and is made of titanium tube; the heating rod (4) is connected to a temperature controller (5) located outside the temperature control barrel (1); the temperature controller (5) is connected to a temperature probe (6); and the temperature probe (6) is arranged in the temperature control barrel (1).

4. The composite environmental stress control system for monitoring aquatic animal behavior according to claim 1, characterized in that: The dissolved oxygen control unit comprises a nitrogen gas source (9), a dissolved oxygen meter (12), a solenoid valve (10), and a gas diffuser A (11); The input end of the solenoid valve (10) is connected to the nitrogen gas source (9), the output end of the solenoid valve (10) is connected to the gas diffuser A (11) through a pipeline, the dissolved oxygen meter (12) is connected to the solenoid valve (10) and controls the opening and closing of the solenoid valve (10), and the gas diffuser A (11) is placed in the equipment placement area (002) of the water basin (24) as the nitrogen output end of the dissolved oxygen control unit.

5. A composite environmental stress control system for monitoring aquatic animal behavior according to claim 4, characterized in that: The dissolved oxygen meter (12) is also connected to a dissolved oxygen sensor (13), and the dissolved oxygen sensor (13) is located in the equipment placement area (002) of the water basin (24).

6. The composite environmental stress control system for monitoring aquatic animal behavior according to claim 1, characterized in that: The carbon dioxide partial pressure control unit comprises a carbon dioxide gas source (18), a pressure reducing valve (17), a carbon dioxide enricher (14), and a gas diffuser B (15); The input end of the pressure reducing valve (17) is connected to the carbon dioxide gas source (18); the carbon dioxide enricher (14) has an air inlet (19), a carbon dioxide inlet interface and a mixed gas outlet interface; the air inlet (19) of the carbon dioxide enricher (14) is connected to the atmosphere; the carbon dioxide inlet interface of the carbon dioxide enricher (14) is connected to the output end of the pressure reducing valve (17) through a pipeline; the mixed gas outlet interface of the carbon dioxide enricher (14) is connected to the gas diffuser B (15) through a pipeline; the gas diffuser B (15) is placed in the equipment placement area (002) of the water basin (24) as the mixed gas output end of the carbon dioxide partial pressure control unit.

7. A composite environmental stress control system for monitoring aquatic animal behavior according to claim 6, characterized in that: The carbon dioxide enricher (14) is also connected to a pH probe (16), and the pH probe (16) is located in the equipment placement area (002) of the water basin (24).

8. The composite environmental stress control system for monitoring aquatic animal behavior according to claim 1, characterized in that: The camera (21) is mounted on a camera bracket (22), and a light source (20) is also provided on the camera bracket (22).

9. The composite environmental stress control system for monitoring aquatic animal behavior according to claim 1, characterized in that: The inner bottom surface of the water basin (24) is white.

10. The composite environmental stress control system for monitoring aquatic animal behavior according to claim 1, characterized in that: Each of the water body passage holes (2301) is located on the same side of the inner partition tube (23), and the water pumping end and water return end of the temperature control unit, the nitrogen output end of the dissolved oxygen control unit, and the mixed gas output end of the carbon dioxide partial pressure control unit are all located on a side of the equipment placement area (002) of the water basin (24) away from each of the water body passage holes (2301).

Images