Method for cultivating low-glycemic rice seeds

Abstract

The invention relates to the technical field of seed cultivation, and provides a low-glycemic rice seed cultivation method which comprises the following steps: S1, selecting a rice seed with a low-glycemic characteristic, and drying in the sun; s2, the rice seeds dried in the S1 are put into clear water to be soaked, then a sterilization agent continues to be added for soaking, and then the rice seeds are taken out and drained; s3, the rice seeds soaked in the clear water in the S2 are put into gibberellic acid liquid to be soaked and then taken out to be drained; s4, the rice seeds in S3 are placed in a constant-temperature box to be subjected to germination acceleration; s5, selecting a farmland, arranging the farmland, and then planting the germinated seeds on the farmland to obtain seedlings; s6, transplanting the seedlings into the paddy field until rice ears are born and seeds are obtained; s7, sampling the seeds in the step S6, performing sugar determination, and selecting the seeds conforming to the low glycemic characteristic; and S8, repeating the steps from S1 to S7 to perform seed cultivation for 2-3 batches, and finally obtaining the pure rice seeds with the low glycemic characteristic.

Description

Technical Field

[0001] The present invention relates to the technical field of seed breeding, and in particular to a method for breeding low-glycemic rice seeds. Background Art

[0002] Rice is native to China and is one of the world's major food crops. China's rice planting area accounts for a quarter of the country's food crops, and the output accounts for more than half. The cultivation history of rice is 15,000 years. As a major food crop, in addition to edible caryopsis, rice can be used to make starch, wine, and vinegar. Rice bran can be used to make sugar, press oil, and extract furfural for industrial and medical use. At present, with the development of agricultural technology, the cultivation of rice seeds has made great progress. These new types of rice cultivation seeds can obtain higher yields;

[0003] For example, the Chinese patent (publication number: CN109744100B) discloses a method for cultivating dryland rice seeds, including a method for arranging rice planting areas and a method for hybrid breeding:

[0004] The layout method of the rice planting area is a method in which paddy fields surround dry fields, comprising the following steps:

[0005] S1: Select a piece of farmland, weed, renovate, add soil conditioner to the farmland, remove the residual fertilizer and pesticide in the farmland, and use it as a rice planting area;

[0006] S2: Divide the planting area into two areas, including a dry field area and a paddy field area, wherein the dry field area is located in the center of the entire planting area, and the paddy field area surrounds the dry field area, is distributed around the dry field area, and is separated from the dry field area by ridges, and the area ratio of the dry field area to the paddy field area is 1:1;

[0007] S3: Irrigate and store water in paddy fields to make the water level 5cm-10cm higher than the planting area, and do not irrigate dry fields”;

[0008] The rice seeds cultivated by this patent can grow in dry fields, breaking through the constraints of geographical conditions to increase, however, rice is the main food crop of Chinese residents, and the sugar content in rice is relatively high. For the diabetic group, they can only reduce the consumption of rice, which will reduce the intake of other nutrients except sugar, thus affecting the health of the diabetic group. In view of this, the present invention proposes a method for cultivating low-glycemic rice seeds. Summary of the invention

[0009] The present invention provides a method for cultivating low-glycemic rice seeds, which solves the problem in the prior art that the high sugar content of rice is not conducive to consumption by diabetic patients.

[0010] The technical solution of the present invention is as follows: A method for cultivating low-glycemic rice seeds comprises the following steps:

[0011] S1: Select rice seeds with low glycemic characteristics and dry them in the sun for 2-3 days in sunny weather;

[0012] S2: Soak the dried rice seeds in S1 in clean water for 2-5 hours, then continue to add a fungicide and soak for 1-2 hours, then take out the rice seeds and drain;

[0013] S3: soaking the rice seeds soaked in the water in S2 in a 10-15 mg / L gibberellic acid solution, taking them out and draining them after soaking for 20-24 hours;

[0014] S4: placing the rice seeds in S3 in a constant temperature box for germination;

[0015] S5: selecting a farmland and tidying up the farmland, and then planting the germinated seeds on the farmland to obtain rice seedlings;

[0016] S6: transplant the seedlings into paddy fields and carry out water management and fertilizer management until rice ears are formed and seeds are obtained;

[0017] S7: Sample the seeds in S6 to measure the sugar content and select the seeds that meet the low glycemic characteristics;

[0018] S8: Repeat steps S1-S7 to carry out 2-3 batches of seed cultivation, and finally obtain pure rice seeds with low glycemic characteristics.

[0019] Preferably, in S2, the fungicide, calculated by mass percentage, includes: 5%-10% of cyanocobalamin, 1%-5% of imidacloprid, and the balance is water.

[0020] Preferably, the specific preparation process of the bactericidal agent is as follows:

[0021] (1) adding cyanothiocarb to water according to the mass percentage, mixing the mixture while stirring to obtain a mixed solution;

[0022] (2) Then, imidacloprid is added into the mixed solution according to the mass percentage and stirred for a second time to obtain a fungicide.

[0023] Preferably, in S4, the specific process of accelerating germination of rice seeds is as follows:

[0024] (1) Adjust the temperature inside the thermostat to 25-30°C for 4-5 hours;

[0025] (2) Then adjust the temperature inside the incubator to 31-35°C for 4-5 hours;

[0026] (3) Then adjust the temperature inside the thermostat to 36-40°C until the rice seeds germinate.

[0027] Preferably, in S5, the specific process of arranging the farmland is as follows:

[0028] (1) Remove weeds from the farmland, moisten the soil after 2-3 days, and then renovate it to ensure that the soil moisture is between 6% and 10%;

[0029] (2) Add soil conditioners to the farmland to remove residual fertilizers and pesticides in the farmland and ensure that the soil will not affect the normal growth of rice seeds.

[0030] Preferably, the soil conditioner is selected from one or more of polyacrylic acid, vinyl acetate maleic acid, and polyvinyl alcohol.

[0031] Preferably, in S6, the water management of the paddy field comprises the following steps:

[0032] (1) Irrigate and store water in the paddy field so that the water level is 5cm-10cm higher than the planting area;

[0033] (2) In the later stage of rice growth, ditches are dug to drain water to control ineffective tillering and increase the ear formation rate.

[0034] Preferably, in S6, the fertilization management of the paddy field comprises the following steps:

[0035] (1) Before transplanting the seedlings, apply sufficient organic base fertilizer to the paddy field with a fertilizer thickness of 1cm-2cm.

[0036] (2) During the growth of seedlings, topdressing should be carried out in a timely manner according to the growth of seedlings and soil fertility. Nitrogen fertilizer should be used as the main fertilizer, supplemented with phosphorus and potassium fertilizers. When applying topdressing, care should be taken to apply small amounts and multiple times to avoid applying too much fertilizer at one time, which may cause seedling burn.

[0037] The working principle and beneficial effects of the present invention are:

[0038] 1. The use of fungicides can effectively kill the pathogens of rice seeds, greatly reducing the disease rate of seeds, thereby increasing the germination rate of rice seeds and further increasing the yield of rice seeds;

[0039] 2. By self-pollinating and screening rice seeds with low glycemic characteristics multiple times, the sugar content in the rice seeds obtained in the final batch gradually decreases, which means that as the number of self-pollination of rice seeds increases, the number of purebred rice seeds with low glycemic characteristics screened out increases, thereby improving the stability of rice seeds with low glycemic characteristics and further increasing the yield of rice with low glycemic characteristics. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0041] Figure 1 This is a statistical table of germination rates of rice seeds in each group in Test Example 1;

[0042] Figure 2 This is a statistical table of the germination rate of rice seeds in each group in the test embodiment 2;

[0043] Figure 3 This is a statistical table of sugar content of each group of rice seeds in Test Example 3. DETAILED DESCRIPTION

[0044] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] Embodiment 1:

[0046] This embodiment proposes a method for cultivating low-glycemic rice seeds, comprising the following steps:

[0047] S1: Select rice seeds with low glycemic characteristics and dry them in the sun for 2 days in sunny weather;

[0048] S2: Soak the dried rice seeds in S1 in clean water for 2 hours, then continue to add a fungicide and soak for 1 hour, then take out the rice seeds and drain them, wherein the fungicide is calculated by mass percentage and includes: 5% cyproconazole, 1% imidacloprid, and the balance is water.

[0049] S3: soaking the rice seeds soaked in the water in S2 in a 10 mg / L gibberellic acid solution, taking them out after soaking for 24 hours and draining them;

[0050] S4: placing the rice seeds in S3 in a constant temperature box for germination. The specific process of germination of the rice seeds is as follows:

[0051] (1) Adjust the temperature inside the thermostat to 25°C for 5 hours;

[0052] (2) Then adjust the temperature inside the incubator to 31°C for 5 hours;

[0053] (3) Adjust the temperature inside the thermostat to 36° C. until the rice seeds germinate;

[0054] S5: selecting a farmland and tidying up the farmland, and then planting the germinated seeds on the farmland to obtain rice seedlings;

[0055] S6: transplant the seedlings into paddy fields and carry out water management and fertilizer management until rice ears are formed and seeds are obtained;

[0056] S7: Sample the seeds in S6 to measure the sugar content and select the seeds that meet the low glycemic characteristics;

[0057] S8: Repeat steps S1-S7 to cultivate three batches of seeds, and finally obtain pure rice seeds with low glycemic characteristics.

[0058] The use of fungicides can effectively kill the pathogens of rice seeds, greatly reducing the disease rate of seeds, thereby increasing the germination rate of rice seeds and further increasing the yield of rice seeds;

[0059] By self-pollinating and screening rice seeds with low glycemic characteristics multiple times, the sugar content in the final batch of rice seeds gradually decreases, which means that as the number of self-pollination of rice seeds increases, the number of purebred rice seeds with low glycemic characteristics screened out increases, thereby improving the stability of rice seeds with low glycemic characteristics and then increasing the yield of rice with low glycemic characteristics.

[0060] Furthermore, in S2, the specific preparation process of the bactericidal agent is as follows:

[0061] (1) adding cyanothiocarb to water according to the mass percentage, mixing the mixture while stirring to obtain a mixed solution;

[0062] (2) Then, imidacloprid is added into the mixed solution according to the mass percentage and stirred for a second time to obtain a fungicide.

[0063] Furthermore, in S5, the specific process of farmland arrangement is as follows:

[0064] (1) Remove weeds from the farmland, moisten the soil after 2-3 days, and then renovate it to ensure that the soil moisture is between 6% and 10%;

[0065] (2) Add soil conditioners to the farmland to remove residual fertilizers and pesticides in the farmland and ensure that the soil will not affect the normal growth of rice seeds.

[0066] Furthermore, the soil conditioner is selected from one or more of polyacrylic acid, vinyl acetate maleic acid, and polyvinyl alcohol.

[0067] Furthermore, in S6, water management of paddy fields includes the following steps:

[0068] (1) Irrigate and store water in the paddy field so that the water level is 5cm-10cm higher than the planting area;

[0069] (2) In the later stage of rice growth, ditches are dug to drain water to control ineffective tillering and increase the ear formation rate.

[0070] Furthermore, in S6, the fertilization management of the paddy field includes the following steps:

[0071] (1) Before transplanting the seedlings, apply sufficient organic base fertilizer to the paddy field with a fertilizer thickness of 1cm-2cm.

[0072] (2) During the growth of seedlings, topdressing should be carried out in a timely manner according to the growth of seedlings and soil fertility. Topdressing should be mainly nitrogen fertilizer, supplemented with phosphorus and potassium. When topdressing, attention should be paid to applying small amounts and multiple times to avoid applying too much fertilizer at one time, which may cause seedling burn.

[0073] Embodiment 2:

[0074] This embodiment proposes a method for cultivating low-glycemic rice seeds, which is different from the first embodiment in that it includes the following steps:

[0075] S1: Select rice seeds with low glycemic characteristics and dry them in the sun for 2.5 days in sunny weather;

[0076] S2: Soak the dried rice seeds in S1 in clean water for 3 hours, then continue to add a fungicide and soak for 1.5 hours, then take out the rice seeds and drain them, wherein the fungicide is calculated by mass percentage and includes: 8% cyproconazole, 3% imidacloprid, and the balance is water.

[0077] S3: soak the rice seeds soaked in the water in S2 in a 12 mg / L gibberellic acid solution, take them out and drain them after soaking for 22 hours;

[0078] S4: placing the rice seeds in S3 in a constant temperature box for germination. The specific process of germination of the rice seeds is as follows:

[0079] (1) Adjust the temperature inside the incubator to 28°C for 4.5 hours;

[0080] (2) Then adjust the temperature inside the incubator to 33°C for 4.5 hours;

[0081] (3) Adjust the temperature inside the thermostat to 38° C. until the rice seeds germinate;

[0082] S5: selecting a farmland and tidying up the farmland, and then planting the germinated seeds on the farmland to obtain rice seedlings;

[0083] S6: transplant the seedlings into paddy fields and carry out water management and fertilizer management until rice ears are formed and seeds are obtained;

[0084] S7: Sample the seeds in S6 to measure the sugar content and select the seeds that meet the low glycemic characteristics;

[0085] S8: Repeat steps S1-S7 to cultivate three batches of seeds, and finally obtain pure rice seeds with low glycemic characteristics.

[0086] Embodiment three:

[0087] This embodiment proposes a method for cultivating low-glycemic rice seeds, which is different from the first embodiment in that it includes the following steps:

[0088] S1: Select rice seeds with low glycemic characteristics and dry them in the sun for 3 days in sunny weather;

[0089] S2: Soak the dried rice seeds in S1 in clean water for 5 hours, then continue to add a fungicide and soak for 2 hours, then take out the rice seeds and drain them, wherein the fungicide is calculated by mass percentage and includes: 15% cyanoacrylate, 5% imidacloprid, and the balance is water.

[0090] S3: soaking the rice seeds soaked in the water in S2 in a 15 mg / L gibberellic acid solution, taking them out after soaking for 20 hours and draining them;

[0091] S4: placing the rice seeds in S3 in a constant temperature box for germination. The specific process of germination of the rice seeds is as follows:

[0092] (1) Adjust the temperature inside the thermostat to 30°C for 5 hours;

[0093] (2) Then adjust the temperature inside the thermostat to 35°C for 5 hours;

[0094] (3) Adjust the temperature inside the thermostat to 40° C. until the rice seeds germinate;

[0095] S5: selecting a farmland and tidying up the farmland, and then planting the germinated seeds on the farmland to obtain rice seedlings;

[0096] S6: transplant the seedlings into paddy fields and carry out water management and fertilizer management until rice ears are formed and seeds are obtained;

[0097] S7: Sample the seeds in S6 to measure the sugar content and select the seeds that meet the low glycemic characteristics;

[0098] S8: Repeat steps S1-S7 to cultivate three batches of seeds, and finally obtain pure rice seeds with low glycemic characteristics.

[0099] Comparative Example 1:

[0100] This comparative example proposes a method for cultivating low-glycemic rice seeds, which is different from the first embodiment in that no bactericide is added in S2.

[0101] Comparative Example 2:

[0102] This comparative example proposes a method for cultivating low-glycemic rice seeds, which is different from the first embodiment in that the rice seeds are not soaked in a gibberellic acid solution.

[0103] Comparative Example 3:

[0104] This comparative example proposes a method for cultivating low-glycemic rice seeds, which is different from the first embodiment in that in S8, steps S1 to S7 are repeated to cultivate only two batches of seeds.

[0105] Comparative Example 4:

[0106] This comparative example proposes a method for cultivating low-glycemic rice seeds, which is different from the first embodiment in that the seed cultivation steps S1-S7 are not repeated.

[0107] Test Example 1:

[0108] The germination rates of the seeds in Example 1, Example 2, Example 3 and Comparative Example 1 were statistically analyzed and calculated using the following formula:

[0109]

[0110] The statistical results are as follows Figure 1 As shown in the table, Figure 1 It can be seen that with the increase in the dosage of fungicides, the germination rate of seeds gradually increases. This shows that fungicides can effectively kill the pathogens of rice seeds, greatly reduce the disease rate of seeds, and thus improve the germination rate of rice seeds.

[0111] Test Example 2:

[0112] The germination rates of the seeds in Example 1 and Comparative Example 2 were statistically analyzed and calculated using the following formula:

[0113]

[0114] The statistical results are as follows Figure 2 As shown in the table, Figure 2 It can be seen that after the seeds of Example 1 were soaked in gibberellic acid solution, the germination rate was significantly increased, which shows that soaking in gibberellic acid solution can significantly improve the germination rate of rice seeds.

[0115] Test Example 3:

[0116] The sugar content of the rice seeds in Example 1, Comparative Example 3 and Comparative Example 4 was detected respectively, and the specific implementation steps are as follows:

[0117] (1) 1 kg of rice seeds from Example 1, Comparative Example 3 and Comparative Example 4 were respectively taken, crushed and placed in three different containers;

[0118] (2) Then, add 100 ml of distilled water to each of the three containers and stir thoroughly to promote the dissolution and extraction of sugars;

[0119] (3) placing the three containers in a centrifuge in sequence for centrifugation, and then filtering to obtain an extract;

[0120] (4) The extract is mixed with anthrone reagent, a color reaction occurs under heating conditions, and then the optical density value is measured by colorimetry to calculate the sugar content.

[0121] The specific calculation results are as follows: Figure 3 As shown by Figure 3 It can be seen that as the number of self-pollination times of rice seeds with low glucose water characteristics increases, the sugar content in the rice seeds obtained in the final batch gradually decreases, which means that as the number of self-pollination times of rice seeds increases, the number of purebred rice seeds with low glucose water characteristics screened out increases, thereby improving the stability of rice seeds with low glucose water characteristics, and then increasing the yield of rice with low glucose water characteristics.

[0122] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for cultivating low-glycemic rice seeds, characterized in that: The steps include: S1: Select rice seeds with low glycemic characteristics and dry them in the sun for 2-3 days in sunny weather; S2: Soak the dried rice seeds in S1 in clean water for 2-5 hours, then continue to add a fungicide and soak for 1-2 hours, then take out the rice seeds and drain; S3: soaking the rice seeds soaked in the water in S2 in a 10-15 mg / L gibberellic acid solution, taking them out and draining them after soaking for 20-24 hours; S4: placing the rice seeds in S3 in a constant temperature box for germination; S5: selecting a farmland and tidying up the farmland, and then planting the germinated seeds on the farmland to obtain rice seedlings; S6: transplant the seedlings into paddy fields and carry out water management and fertilizer management until rice ears are formed and seeds are obtained; S7: Sample the seeds in S6 to measure the sugar content and select the seeds that meet the low glycemic characteristics; S8: Repeat steps S1-S7 to carry out 2-3 batches of seed cultivation, and finally obtain pure rice seeds with low glycemic characteristics.

2. The method for cultivating low-glycemic rice seeds according to claim 1, characterized in that: In S2, the fungicide is calculated by mass percentage and includes: 5%-10% of cyanoacrylate, 1%-5% of imidacloprid, and the balance is water.

3. The method for cultivating low-glycemic rice seeds according to claim 2, characterized in that: The specific preparation process of the bactericidal agent is as follows: (1) adding cyanothiocarb to water according to the mass percentage, mixing the mixture while stirring to obtain a mixed solution; (2) Then, imidacloprid is added into the mixed solution according to the mass percentage and stirred for a second time to obtain a fungicide.

4. The method for cultivating low-glycemic rice seeds according to claim 1, characterized in that: In S4, the specific process of germination of rice seeds is as follows: (1) Adjust the temperature inside the thermostat to 25-30°C for 4-5 hours; (2) Then adjust the temperature inside the incubator to 31-35°C for 4-5 hours; (3) Then adjust the temperature inside the thermostat to 36-40°C until the rice seeds germinate.

5. The method for cultivating low-glycemic rice seeds according to claim 1, characterized in that: In S5, the specific process of farmland preparation is as follows: (1) Remove weeds from the farmland, moisten the soil after 2-3 days, and then renovate it to ensure that the soil moisture is between 6% and 10%; (2) Add soil conditioners to the farmland to remove residual fertilizers and pesticides in the farmland and ensure that the soil will not affect the normal growth of rice seeds.

6. The method for cultivating low-glycemic rice seeds according to claim 5, characterized in that: The soil conditioner is selected from one or more of polyacrylic acid, vinyl acetate maleic acid, and polyvinyl alcohol.

7. The method for cultivating low-glycemic rice seeds according to claim 1, characterized in that: In S6, water management of paddy fields includes the following steps: (1) Irrigate and store water in the paddy field so that the water level is 5cm-10cm higher than the planting area; (2) In the later stage of rice growth, ditches are dug to drain water to control ineffective tillering and increase the ear formation rate.

8. The method for cultivating low-glycemic rice seeds according to claim 7, characterized in that: In S6, the fertilization management of paddy fields includes the following steps: (1) Before transplanting the seedlings, apply sufficient organic base fertilizer to the paddy field with a fertilizer thickness of 1cm-2cm. (2) During the growth of seedlings, topdressing should be carried out in a timely manner according to the growth of seedlings and soil fertility. Nitrogen fertilizer should be used as the main fertilizer, supplemented with phosphorus and potassium fertilizers. When applying topdressing, care should be taken to apply small amounts and multiple times to avoid applying too much fertilizer at one time, which may cause seedling burn.

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