Biological membrane material synthesized based on biological algae resource protein as well as preparation method and application of biological membrane material

Abstract

The invention relates to the technical field of biological membranes, in particular to a biological membrane material based on biological algae resource protein synthesis as well as a preparation method and application of the biological membrane material. Uniformly mixing protein and a cross-linking agent, and carrying out cross-linking reaction to obtain a solution; and coating the surface of the seed with the solution, and curing to obtain the biological membrane material. According to the biological membrane material synthesized based on the biological algae resource protein as well as the preparation method and the application of the biological membrane material, a film structure with high mechanical strength and biodegradability is formed by regulating and controlling reaction conditions, and the material can coat seeds, can be used as a carrier of agricultural chemicals, provides a slow release function and nutrition support, and has a good application prospect. The disease and pest resistance of crops is enhanced, and the use amount of chemicals is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of biofilm materials, and in particular relates to a biofilm material synthesized based on biological algae resource protein, and a preparation method and application thereof. Background Art

[0002] With the rapid development of agricultural production, seed coating technology has become an important tool to improve crop yields and farmland management efficiency. By covering the surface of seeds with a protective film, seed coating agents can not only enhance the storage resistance of seeds, but also improve the germination rate and play a role in controlling pests and diseases. However, most seed coating agents on the market currently use synthetic materials, especially microplastics. These materials are difficult to degrade in the natural environment, and their long-term presence may lead to plastic microparticle pollution and cause irreversible damage to the ecosystem. For example, microplastics may penetrate into the soil, destroy the soil structure and microbial ecology, and then affect the growth environment of crops. In addition, microplastics may also migrate through water bodies, pollute rivers, lakes and oceans, and even cause potential harm to human health through the food chain.

[0003] In view of the serious environmental impact of microplastic pollution, many countries and regions have begun to gradually ban or restrict the use of microplastics in agriculture and other fields. For example, the European Union and some developed countries have successively introduced regulations on microplastics to restrict their use in cosmetics, fertilizers and pesticides. This trend shows that the materials used in agricultural production in the future must meet environmental protection and degradable requirements, provide safe and green solutions, and avoid secondary pollution to the environment. In this context, it is imperative to develop a green seed coating agent that can replace microplastics. Summary of the invention

[0004] The purpose of the present invention is to provide a biofilm material based on protein synthesis from biological algae resources, and a preparation method and application thereof. By regulating the reaction conditions, a film structure with high mechanical strength and biodegradability is formed. The material can coat seeds and can be used as a carrier of agricultural chemicals to provide sustained-release function and nutritional support, enhance the disease and pest resistance of crops, and reduce the use of chemicals.

[0005] The above technical objectives of the present invention are achieved through the following technical solutions:

[0006] The present invention provides a method for preparing a biofilm material based on protein synthesis from biological algae resources, comprising the following steps:

[0007] Extracting proteins from algae;

[0008] Mixing the protein and the cross-linking agent uniformly to perform a cross-linking reaction to obtain a solution;

[0009] The solution is coated on the surface of seeds and solidified to obtain a biofilm material.

[0010] Furthermore, before the protein and the cross-linking agent are evenly mixed, soluble fertilizers, amino acids and / or trace elements are added to the protein.

[0011] Furthermore, after the protein and the cross-linking agent are uniformly mixed, pesticides, insecticides and / or fungicides are added to the protein.

[0012] Furthermore, the protein is extracted by alkaline extraction or acid extraction.

[0013] Furthermore, the cross-linking agent is a mixture of any one or more of glutaraldehyde, chitosan or sodium alginate.

[0014] Furthermore, the step of uniformly mixing the protein and the cross-linking agent includes uniformly mixing a protein having a solution concentration of 1-5% (w / v) and a cross-linking agent having a solution concentration of 1-2% (v / v).

[0015] Furthermore, the biological algae is Chlorella or Spirulina.

[0016] Furthermore, the curing is performed by heat curing at 40 to 60°C.

[0017] The present invention also provides a biofilm material synthesized based on biological algae resource protein, which has the same technical effect.

[0018] The present invention also provides an application of a biofilm material synthesized based on biological algae resource protein, and the prepared biofilm material is applied to seed coating of different types of crops, with the same technical effect.

[0019] In summary, the present invention has the following beneficial effects:

[0020] (1) The biofilm material provided by the present invention uses renewable algae resources as raw materials, is made through protein extraction and cross-linking technology, and is completely biodegradable. Compared with traditional microplastic seed coating agents, this algae-based biofilm can be completely degraded in the natural environment, avoiding the pollution of soil and water bodies by microplastic particles and reducing ecological risks. The use of algae resources reduces dependence on fossil fuels and synthetic chemicals, while complying with current environmental regulations and sustainable development requirements, and helps promote the green transformation of the agricultural industry. In addition, the growth process of algae does not require pesticides or fertilizers, and does not occupy arable land resources, further reducing its negative impact on the environment.

[0021] (2) Algae protein is rich in nutrients such as amino acids, vitamins and minerals. By integrating these natural nutrients into biofilm materials, it can provide early nutritional support for seed germination, effectively improving the germination rate of seeds and the healthy growth of seedlings. Compared with traditional seed coating agents, this algae-based biofilm not only provides physical protection, but also serves as a nutrient source to enhance the vitality and stress resistance of seeds, thereby helping to increase agricultural yields. In addition, the natural biocompatibility of algae protein means that it will not produce harmful substances when degraded in the soil, is friendly to the soil microecological environment, and can continuously provide support for crop growth.

[0022] (3) The biofilm material provided by the present invention has good carrier function and can be used as a carrier of agricultural chemicals (such as pesticides, fungicides and fertilizers, etc.) to achieve a slow-release effect. By coating agricultural chemicals in the biofilm, its release rate can be controlled so that the chemicals are gradually released during the critical period of plant growth, thereby extending the duration of the efficacy. The slow-release function not only improves the utilization efficiency of agricultural chemicals and reduces the number of applications, but also avoids environmental pollution and plant damage caused by a one-time high-concentration release. In addition, the application of slow-release technology reduces the accumulation of chemicals in the soil and reduces the risk of groundwater pollution. DETAILED DESCRIPTION

[0023] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, a biofilm material based on protein synthesis of biological algae resources and its preparation method and application proposed by the present invention, its specific implementation method, characteristics and effects are described in detail as follows.

[0024] This specific embodiment provides a method for preparing a biofilm material based on protein synthesis of biological algae resources, comprising the following steps:

[0025] Extracting proteins from algae;

[0026] Mixing the protein and the cross-linking agent uniformly to perform a cross-linking reaction to obtain a solution;

[0027] The solution is coated on the surface of seeds and solidified to obtain a biofilm material.

[0028] It is understandable that the method for synthesizing biofilm materials based on biological algae resource proteins mainly uses proteins extracted from algae as the main raw material, and forms a cross-linked network through a chemical reaction with a cross-linking agent to generate a material with biofilm properties. Algae are rich in high-content proteins (such as spirulina, large algae, etc.), which are rich in active groups (such as amino, carboxyl, hydroxyl, etc.) and are suitable as basic components of biofilm materials. The extracted protein is mixed with a specific cross-linking agent to undergo a chemical cross-linking reaction. The cross-linking agent connects the protein molecular chains through covalent bonds to form a three-dimensional network structure. This network structure gives the biofilm material stability and mechanical strength; the formed cross-linking solution is evenly coated on the seed surface and forms a biofilm material by curing. After curing, the cross-linking between the protein molecules is further strengthened to form a membrane material with a certain thickness, toughness and functionality.

[0029] Algae proteins contain active groups. By selecting different cross-linking agents and adjusting the cross-linking conditions, the mechanical properties (such as flexibility and strength) and functional properties (such as air permeability and biodegradability) of biofilm materials can be adjusted to meet various needs. Applying biofilm on the surface of seeds can protect the seeds from mechanical damage and external environmental stress (such as water loss and pathogen invasion). At the same time, it can also regulate the water and nutrient supply required for seed germination to a certain extent.

[0030] In some preferred embodiments, before the protein and the cross-linking agent are evenly mixed, soluble fertilizers, amino acids and / or trace elements are added to the protein.

[0031] It can be understood that the process of adding soluble fertilizers, amino acids and / or trace elements to the protein before the protein and the cross-linking agent are evenly mixed is actually the introduction of additional functional components into the biofilm material.

[0032] Soluble fertilizers can provide a large amount of elements (such as nitrogen, phosphorus, and potassium) required for plant growth, and release nutrients during seed germination and early growth stages; amino acids are organic nutrients for plant growth and are also the building blocks of protein. Adding amino acids can increase the germination rate and stress resistance of seeds. At the same time, they can also act as part of the protein network and interact with cross-linking agents to further optimize the performance of membrane materials; trace elements include but are not limited to iron, zinc, copper, boron, etc. Trace elements are essential components for plant growth and can promote the healthy growth of plants through a slow-release effect in the early stages of seed germination.

[0033] The biofilm material provided in this specific embodiment forms a stable three-dimensional cross-linked network structure after coating and curing. This structure not only protects the seeds, but also acts as a slow-release carrier to gradually release the added fertilizers, amino acids or trace elements into the soil, ensuring that the seeds continue to obtain nutrient support during the growth process; the network structure formed by the reaction of protein and cross-linking agent can wrap fertilizers, amino acids and trace elements, so that they are evenly distributed in the biofilm. During seed germination or water absorption, these functional components can be slowly released to avoid nutrient loss or excessive fertilizer effect due to direct fertilization; the carboxyl or amino group in the amino acid, as well as certain trace elements (such as zinc and iron) can act as catalysts or participate in the cross-linking reaction of the protein, further improving the mechanical properties and stability of the biofilm.

[0034] After adding fertilizers and amino acids to the seed coating, the above-mentioned additives can provide sufficient nutritional support for seedlings and significantly enhance the growth vitality and survival rate of seeds in the early stage after sowing; the network structure of the biofilm material can achieve the slow release of nutrients, avoid the waste caused by too fast release of nutrients, and reduce the pollution to the environment caused by excessive fertilization; the introduction of fertilizers and trace elements into the biofilm can form a more effective protective layer on the seed surface, reduce the direct impact of the external environment (such as dryness, pests and diseases) on the seeds, and provide the nutrients required for seed germination.

[0035] In some preferred embodiments, pesticides, insecticides and / or fungicides are added to the protein before the protein and the cross-linking agent are evenly mixed.

[0036] It is understandable that pesticides, insecticides and / or fungicides are added before the protein and the cross-linking agent are evenly mixed, and these chemical agents are evenly distributed and wrapped in the biofilm using the network structure of the biofilm material, thereby achieving the protective release and synergistic function of the agent. After adding pesticides, insecticides or fungicides to the protein solution, they are evenly distributed through the mixing process. The protein solution has good dispersibility and can effectively wrap the agent particles to avoid uneven distribution or loss of the agent on the seed surface; after the protein reacts with the cross-linking agent, a three-dimensional cross-linked network is formed, and the pesticides, insecticides and fungicides are embedded or wrapped in the structure of the biofilm. This physical embedding and chemical fixation method can prevent the rapid release of the agent and provide a sustained release effect; the mesh structure of the biofilm material provided in this specific embodiment has a certain controlled release ability. Under the moisture, temperature or other stimulation conditions in the environment, the agent will be gradually released from the biofilm to ensure long-term efficacy. The pesticides, insecticides or fungicides are evenly distributed in the biofilm and can directly form a protective layer on the surface of the seeds, effectively blocking the invasion of pathogens, pests or fungi in the external environment on the seeds.

[0037] In some preferred embodiments, the protein is extracted by alkaline extraction or acid extraction.

[0038] During the extraction process, controlling the extraction conditions (e.g., pH value, temperature) can optimize the extraction efficiency and quality of the protein. When using the alkaline extraction method, the algae powder is dissolved in an appropriate amount of alkaline solution (e.g., 0.1-0.5M sodium hydroxide solution), stirred to dissolve, and then insoluble impurities are removed by filtration or centrifugation. The extracted solution is further purified by dialysis or other purification methods to obtain a high-purity protein solution.

[0039] In some preferred embodiments, in the above-mentioned post-extraction step, the protein solution is further purified to remove non-protein components such as lipids and polysaccharides, thereby improving purity and stability, making the quality of the final membrane material more uniform and the degradation performance better.

[0040] It is understandable that the alkaline extraction method can cause the protein to unfold or partially dissociate under alkaline conditions, exposing more active groups (such as amino and carboxyl groups), which is helpful for the subsequent reaction with the cross-linking agent.

[0041] Controlling extraction conditions (such as pH and temperature) can avoid protein denaturation or degradation, thereby retaining its natural structure and function, thereby improving the reaction efficiency of protein and cross-linker, forming a more stable three-dimensional cross-linked network, and enhancing the durability of the biofilm. The exposed active groups can also increase the functionality of the biofilm, such as regulating permeability, hygroscopicity or biodegradability.

[0042] The pH value and temperature control during the extraction process not only affect the extraction efficiency of the protein, but also affect the molecular weight distribution and structural morphology of the protein. Proteins with different properties can be obtained through different extraction conditions (such as alkali concentration, time, temperature, etc.); different protein molecular weights or structures can form different cross-linking densities, giving the biofilm material specific properties (such as flexibility, water permeability, degradation rate, etc.) to meet different application requirements. By adjusting the extraction conditions, biofilm materials with customized performance can be prepared for different scenarios.

[0043] It should be noted that at an alkali concentration of 0.1 to 0.5 M, alkaline conditions can fully destroy the structure of the algae cell wall, dissolve the protein in the cell, and release it from the cell into the solution. Alkali in this concentration range can effectively promote the dissolution of protein while avoiding the negative effects of too high or too low alkali. If the alkali concentration is insufficient (<0.1 M), the cell wall is not completely destroyed, resulting in incomplete extraction. If the concentration is too high (>0.5 M), the protein will be denatured or degraded, losing its functionality.

[0044] In some preferred embodiments, the cross-linking agent is a mixture of any one or more of glutaraldehyde, chitosan or sodium alginate.

[0045] It is understandable that glutaraldehyde is a dialdehyde compound, and the aldehyde groups (–CHO) at both ends of the molecule can react with the amino groups (–NH2) in the protein to form a stable covalent bond. Glutaraldehyde is highly reactive and can quickly form a three-dimensional cross-linked network with protein molecules; chitosan is a natural polysaccharide extracted from chitin, and its molecular structure contains abundant amino groups and hydroxyl groups, which can form cross-links with carboxyl groups (–COOH) or other active groups in proteins. Chitosan has natural antibacterial properties. Adding chitosan to biofilm materials can give them the ability to inhibit pathogens and microorganisms; sodium alginate is a natural polysaccharide extracted from seaweed, and its molecular structure contains abundant carboxyl groups (–COOH), which can form cross-links with amino groups in proteins. Sodium alginate can form a dense gel network by combining with proteins or other components, thereby improving the strength and stability of the biofilm. Through the addition of sodium alginate, the biofilm can achieve slow degradation and sustained release of nutrients (or agents) under certain conditions, which is suitable for seed coating and agricultural use.

[0046] In some preferred embodiments, the biological algae is Chlorella or Spirulina.

[0047] It is understandable that Chlorella and Spirulina are the two types of microalgae with the highest protein content among algae. Chlorella: the protein content accounts for about 50% to 60% of its dry weight; Spirulina: the protein content is even higher, up to 60% to 70%. These proteins are rich in amino acids, especially functional amino acids such as lysine and arginine, and have higher active groups (such as amino groups and carboxyl groups).

[0048] In some preferred embodiments, the curing is performed by heat curing at 40-60°C.

[0049] In some preferred embodiments, the prepared biofilm material is applied to seed coating of different types of crops.

[0050] The present invention will be further described below in conjunction with specific embodiments.

[0051] Example 1

[0052] In this Example 1, the biological algae protein-based biofilm material is applied to the preparation of a coating agent for field crop (corn) seeds and its field application, so as to achieve nutrient supply for early seed growth, pest and disease control, and improvement of field planting performance.

[0053] The preparation method of the biofilm material of this embodiment 1 is as follows:

[0054] S1. Extracting protein from algae by alkaline extraction: dissolving dried spirulina powder in 0.2M sodium hydroxide solution, stirring and extracting at 50°C for 2 hours, filtering the extract to remove insoluble impurities, dialysis and purification of the extract to remove small molecular impurities, and obtaining a high-purity protein solution. The purity of the extracted protein solution reaches more than 90%;

[0055] S2. Add lysine, glutamic acid and potassium phosphate (0.1 M each) to the protein solution and stir evenly;

[0056] S3, using chitosan as a cross-linking agent, dissolving chitosan in a 1% by mass acetic acid solution, adjusting the pH value to 7.5, mixing the extracted Spirulina protein solution with chitosan, and reacting at 30° C. for 2 hours to form a stable cross-linking network;

[0057] S4, adding 0.2% by mass of matrine solution into step S3, and stirring evenly;

[0058] S5. The protein-based biofilm solution prepared in step S4 is evenly coated on the surface of the corn seeds, and dried at 40° C. to form a dense biofilm coating.

[0059] The protein and the cross-linking agent are mixed evenly, including mixing a 5% (w / v) solution concentration of the protein and a 2% (v / v) concentration of the cross-linking agent evenly.

[0060] Example 2

[0061] This Example 2 uses Chlorella protein-based biofilm as the core material and is applied to tomato seed coating to improve seed germination rate, seedling survival rate and nutrient utilization efficiency, while achieving slow-release supply of fertilizers and trace elements, and enhancing seed resistance and growth performance.

[0062] The preparation method of the biofilm material of this embodiment 2 is as follows:

[0063] S1. Add the dried Chlorella powder to 0.1M hydrochloric acid solution, stir and extract at 45°C for 2 hours to destroy the cell wall and dissolve the protein, centrifuge the extract (3000 rpm, 10 minutes) to remove insoluble impurities, and dialysis the extract to remove small molecule impurities and salts to obtain a high-purity protein solution;

[0064] S2, dissolve the trace elements (0.05 MCaCl2 and 0.01 MMgSO4 solution) directly in the protein solution;

[0065] S3, using sodium alginate as a cross-linking agent, the extracted protein solution was mixed with 2% sodium alginate solution in a volume ratio of 2:1, 0.05 M CaCl2 solution was added as a curing trigger of the cross-linking agent, and the mixture was reacted at room temperature (25°C) for 30 minutes;

[0066] S4, dispersing the slow-release fertilizer in the form of particles into step S3 and stirring evenly;

[0067] S5. Evenly coat the prepared protein / sodium alginate mixture on the surface of tomato seeds, use a drum coating machine to further evenly distribute the coating solution, and ensure that the fertilizer particles are embedded in the membrane material, dry at a low temperature of 40°C to prevent nutrient degradation, and finally form a dense biofilm layer.

[0068] The protein and the cross-linking agent are mixed evenly, including mixing a 3% (w / v) solution concentration of the protein and a 1% (v / v) concentration of the cross-linking agent evenly.

[0069] Example 3

[0070] This embodiment 3 is a strawberry seed coating technology based on algae protein biofilm materials to meet the high nutritional needs of strawberry seeds and the requirements of disease prevention and control, while achieving environmental protection and controllable degradation, thereby improving strawberry planting efficiency and economic benefits.

[0071] The preparation method of the biofilm material of this embodiment 2 is as follows:

[0072] S1. A double extraction method (combination of alkaline extraction and acid extraction) was used to extract the protein and functional components in the algae to the maximum extent: the mixed algae powder was dissolved in a 0.2M NaOH solution, stirred and extracted at 50°C for 2 hours, insoluble impurities were removed by filtration, the protein solution was collected, the residue was treated again with a 0.1M hydrochloric acid solution, extracted at 40°C for 1 hour, centrifuged, the acid-extracted protein solution was collected, the protein solutions obtained by the alkaline extraction and the acid extraction were mixed, and impurities and salts were removed by purification by dialysis to obtain a high-purity protein solution;

[0073] S2, dissolving 0.05 M zinc sulfate, 0.1 M potassium phosphate and amino acids directly in the protein solution;

[0074] S3, using a composite crosslinking agent of chitosan and glutaraldehyde, dissolving chitosan in a 1% acetic acid solution, mixing a glutaraldehyde solution (mass fraction 0.2%) and a chitosan solution in a ratio of 1:4, adding the extracted algae protein solution, and reacting at 40° C. and pH 6.8 for 2 hours to allow the protein to fully react with the crosslinking agent to form a high-strength three-dimensional network structure;

[0075] S4, adding an antifungal agent (carbendazim solution, mass fraction 0.1%) to step S3, slowly dripping the antifungal agent and stirring evenly;

[0076] S5. The prepared biofilm solution is evenly coated on the surface of the strawberry seeds by a drum coating machine, and naturally air-dried at 30°C to ensure that the film material is fully fitted to the seeds to form a dense protective layer.

[0077] The protein and the cross-linking agent are mixed evenly, including mixing the protein with a solution concentration of 4% (w / v) and the cross-linking agent with a concentration of 1.5% (v / v).

[0078] Performance Testing

[0079] The seeds obtained in Examples 1 to 3 were sown in the field for germination test, and the germination rate, seedling growth rate and incidence of diseases and insect pests were observed. The test results are as follows:

[0080]

[0081]

[0082] From the above results, it can be seen that corn seeds had the highest germination rate (95%), because the addition of amino acids and potassium phosphate promoted early nutrient supply. The germination rates of tomato seeds and strawberry seeds were 90% and 92%, respectively, which were slightly lower but still at an excellent level.

[0083] Seedling survival rate: Strawberry seeds had the highest seedling survival rate (94%) because the combination of composite cross-linking agents and slow-release nutrients improved the growth vitality of seeds in the later stage of germination. The survival rates of corn and tomato seedlings were both 90%, indicating that biofilm technology can effectively improve seed viability after germination.

[0084] Pest and disease control: Due to the addition of biological pesticides and antifungal agents to corn seeds and strawberry seeds, the incidence of pests and diseases is reduced to below 20% and below 10% respectively. Since no disease control ingredients are added to tomato seeds, the incidence of pests and diseases has not changed significantly, which shows the importance of adding disease-resistant ingredients.

[0085] Fertilizer utilization efficiency: Tomato and strawberry seeds showed higher fertilizer utilization efficiency, mainly due to the slow-release fertilizer technology. Although the fertilizer utilization efficiency of corn seeds was significantly improved, it was slightly inferior to the other two.

[0086] In terms of soil degradability, strawberry seeds use a composite cross-linking agent of chitosan and glutaraldehyde, which has a controllable degradation rate and is completely environmentally friendly, and performs best. Corn and tomato seed coating materials also have good degradation properties, but the degradation time is longer.

[0087] Corn seed coating is more suitable for large-scale cultivation of field crops, emphasizing high-strength film layer and disease and pest resistance. Tomato seed coating focuses on nutrient supply and slow-release fertilizer, and is suitable for horticultural cultivation. Strawberry seed coating is suitable for high-value-added economic crops, taking into account nutrition, disease resistance and environmental protection performance.

[0088] From the above comparison, it can be seen that all three implementation cases have achieved significant improvements, especially in tomatoes and strawberries. Corn and strawberry seeds performed better by adding disease-resistant ingredients, while tomato seeds did not have sufficient prevention and control capabilities because they did not add relevant ingredients. Strawberry seed coating technology is most suitable for high value-added crops, corn is more suitable for large-scale planting scenarios, and tomatoes are both nutritious and environmentally friendly among horticultural crops.

[0089] The above implementation cases demonstrate the application of the biofilm material of the present invention in the coating of different crop seeds, and confirm its effectiveness in providing nutrition, slow-release chemicals and enhancing seedling health. By adjusting the source of algae protein, the type of cross-linking agent and the reaction conditions, the biofilm material of the present invention can be widely used in a variety of crops, and has good scalability and market application potential. The above is only a preferred embodiment of the present invention, and does not limit the present invention in any form. Although the present invention has been shown as a preferred embodiment as above, it is not used to limit the present invention. Any technician familiar with this profession can make some changes or modifications to equivalent embodiments of equivalent changes without departing from the scope of the technical solution of the present invention using the technical content disclosed above. However, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still belongs to the scope of the technical solution of the present invention.

Claims

1. A method for preparing a biofilm material based on protein synthesis from biological algae resources, characterized in that: The steps include: Extracting proteins from algae; Mixing the protein and the cross-linking agent uniformly to perform a cross-linking reaction to obtain a solution; The solution is coated on the surface of seeds and solidified to obtain a biofilm material.

2. The method for preparing a biofilm material based on protein synthesis from biological algae resources according to claim 1, characterized in that: Before the protein and the cross-linking agent are evenly mixed, soluble fertilizers, amino acids and / or trace elements are added to the protein.

3. The method for preparing a biofilm material based on protein synthesis from biological algae resources according to claim 1, characterized in that: After the protein and the cross-linking agent are uniformly mixed, pesticides, insecticides and / or fungicides are added to the protein.

4. The method for preparing a biofilm material based on protein synthesis from biological algae resources according to claim 1, characterized in that: The protein is extracted by alkali extraction or acid extraction.

5. The method for preparing a biofilm material based on protein synthesis from biological algae resources according to claim 1, characterized in that: The cross-linking agent is any one or more of glutaraldehyde, chitosan or sodium alginate.

6. The method for preparing a biofilm material based on protein synthesis from biological algae resources according to claim 1, characterized in that: The step of uniformly mixing the protein and the cross-linking agent comprises uniformly mixing the protein with a solution concentration of 1-5% (w / v) and the cross-linking agent with a concentration of 1-2% (v / v).

7. The method for preparing a biofilm material based on protein synthesis from biological algae resources according to claim 1, characterized in that: The biological algae is Chlorella or Spirulina.

8. The method for preparing a biofilm material based on protein synthesis from biological algae resources according to claim 1, characterized in that: The curing is performed by heat curing at 40 to 60°C.

9. A biofilm material synthesized based on protein from biological algae resources according to any one of claims 1 to 8.

10. The use of a biofilm material based on protein synthesis from biological algae resources according to claim 9, characterized in that: The prepared biofilm material is applied to seed coating of different types of crops.

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