Method for regulating and controlling plant energy metabolism and application thereof

Abstract

The invention relates to the technical field of plant electrical response, in particular to a method for regulating and controlling plant energy metabolism and application thereof.According to the method, plant energy metabolism is regulated and controlled through electrical bitaxis of plants, directional regulation and control of plant energy metabolism are achieved, the plant energy utilization efficiency and growth and development are improved, and the plant energy utilization rate is increased. The problem that in the prior art, plant energy metabolism cannot be directionally adjusted is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of plant electrical response, and in particular to a method for regulating plant energy metabolism and application thereof. Background Art

[0002] Glycolysis and tricarboxylic acid cycles are important energy metabolism pathways in plant cells. By regulating the glycolysis and tricarboxylic acid cycles in plants, biological functions and metabolism can be regulated, the energy utilization efficiency of plant cells can be improved, and plant growth and development can be promoted. However, there is currently no method that can specifically regulate the glycolysis and tricarboxylic acid cycles in plants. Summary of the invention

[0003] The purpose of the present invention is to provide a method for regulating plant energy metabolism and its application.

[0004] The technical solution is as follows:

[0005] The invention provides a method for regulating plant energy metabolism, which utilizes the electrical diphotaxis of plants to regulate plant energy metabolism.

[0006] Furthermore, the energy metabolism of plants can be regulated by regulating the tendency of plant roots toward the cathode and anode.

[0007] Furthermore, the plants are cultured in a closed-loop system with a culture matrix, and the voltage and current are adjusted to determine the voltage and current ranges when the plant roots tend toward the cathode and anode respectively; the root electrotropism is regulated according to the determined voltage and current ranges, thereby achieving directional control of plant energy metabolism.

[0008] Further, S1, two metal plates are inserted in parallel on both sides of a rectangular water tank, the two metal plates are respectively connected to the positive electrode and the negative electrode of a DC power supply, a culture matrix is ​​added to the water tank to form a closed loop, seeds are placed on the sponge and covered with a double layer of gauze, and then the sponge is placed in the culture matrix of the water tank to culture the seeds;

[0009] S2, by adjusting the voltage intensity applied to both sides of the culture matrix and the current intensity flowing through the culture matrix, determine the voltage and current ranges when the plant roots tend toward the cathode and the anode respectively;

[0010] S3, based on demand, uses the voltage and current of the plant root system to exhibit anode or cathode tropism to systematically regulate plant energy metabolism.

[0011] The present invention also includes the application of any of the above-mentioned methods, characterized by: application in the fields of biology and crop science.

[0012] Compared with the prior art, the present invention utilizes the electrical diphotaxis of plants to regulate plant energy metabolism, thereby achieving directional regulation of plant energy metabolism, improving plant energy utilization efficiency and growth and development, and solving the problem that the prior art cannot directionally regulate plant energy metabolism.

[0013] Instruction Manual

[0014] Figure 1 FIG. 4 is a diagram of an experimental device used in an embodiment.

[0015] Figure 2 This is a graph showing the results of plant root electrotropism under different currents in an embodiment. DETAILED DESCRIPTION

[0016] The technical scheme of the present invention is further described below in conjunction with specific examples. It should be understood that the following examples are only exemplary descriptions and explanations of the present invention, and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are included in the scope that the present invention is intended to protect.

[0017] Example 1

[0018] S1, insert two stainless steel plates in parallel on both sides of the rectangular transparent organic board water tank, the two stainless steel plates are respectively connected to the positive and negative electrodes of the DC power supply, add distilled water into the water tank to form a closed loop, place the seeds on the sponge and cover it with a double layer of gauze, and then put the sponge into the distilled water in the water tank to culture the seeds;

[0019] S2, by adjusting the voltage intensity applied to both sides of the culture matrix, determine the voltage range in which the roots growing from the seeds show cathodic tendency;

[0020] S3, select any voltage value within the above voltage range, at which the conductivity of the culture matrix is ​​changed to adjust the current intensity, and determine that the roots grown from the seeds show an anode-biased current range A1 and a cathode-biased current range A2.

[0021] S4, based on demand, uses the voltage and current of the plant root system to exhibit anode or cathode tropism to systematically regulate plant energy metabolism.

[0022] Example 2

[0023] (1) See Figure 1 , wheat (Yannong No. 19) seeds were evenly placed on 6 sponges with a thickness of 2 cm, with a spacing of 1 cm between adjacent seeds. They were covered with double-layer gauze and placed in 6 water tanks (30×10×10 cm). The culture medium of the 6 water tanks was 1L of distilled water.

[0024] (2) Two stainless steel plates (10×10×0.1 cm) were inserted in parallel at both ends of each water tank, and then connected to the anode and cathode of the electrophoresis instrument respectively. The water tank voltage was controlled by the electrophoresis instrument, and the set voltages of the five water tanks were 1V, 5V, 10V, 20V, and 30V, respectively. The remaining treatment group was not powered on and served as a control.

[0025] (3) After 6 days of incubation at 20±0.1℃ in the dark, record the root tropism:

[0026] The number of roots growing toward the cathode and the anode in the control group was the same. When the applied voltage was 1V, the root growth direction was not affected. When the applied voltage was 5V, 80% of the roots were observed to grow toward the cathode. When the applied voltage was 10V, all the roots were observed to grow toward the cathode. When the applied voltage was 20V, all the roots were observed to grow toward the cathode, but the root growth was significantly inhibited. When the applied voltage was 30V, the roots were severely inhibited and could not grow normally. Therefore, the voltage condition for wheat roots to show cathode-oriented behavior is 5-20V.

[0027] Example 3

[0028] (1) Wheat (Yannong No. 19) seeds were evenly placed on 6 sponges with a thickness of 2 cm, with a spacing of 1 cm between adjacent seeds. They were covered with double-layer gauze and placed in 6 water tanks (30×10×10 cm). Mixtures with different conductivity were prepared by changing the ratio of distilled water and tap water. The culture matrix of the 6 water tanks was 1L of distilled water, distilled water, mixture 1 of distilled water and tap water, mixture 2 of distilled water and tap water, mixture 3 of distilled water and tap water, and tap water.

[0029] (2) Two stainless steel plates (10×10×0.1 cm) were inserted in parallel at both ends of each water tank and then connected to the anode and cathode of the electrophoresis apparatus respectively. One of the treatment groups with only distilled water was not energized as a control. The other five groups were all applied with a voltage of 10 V and current intensities of 0.005 mA, 0.3 mA, 0.5 mA, 1 mA, and 2 mA, respectively.

[0030] (3) After 6 days of incubation at 20±0.1℃ in the dark, take photos and record the root tropism.

[0031] The results showed that the amount of growth in the control group towards the cathode and towards the anode was the same; see Figure 2, in the treatment group with a current intensity of 0.005mA, all roots were observed to grow toward the cathode; in the treatment group with a current intensity of 0.3mA, 60% of the roots were observed to grow toward the cathode; in the treatment group with a current intensity of 0.5mA, 80% of the roots were observed to grow toward the anode; in the treatment group with a current intensity of 1mA, all roots were observed to grow toward the anode; in the treatment group with a current intensity of 2mA, all roots were observed to grow toward the anode, and the root growth was significantly inhibited. Therefore, the current condition for wheat roots to show cathode-oriented behavior is greater than 0-0.3mA (excluding 0), and the current condition for showing anode-oriented behavior is 0.5-2mA.

[0032] Example 4

[0033] Based on the electrical response characteristics of wheat roots showing electroditropism, the energy metabolism is regulated in the following steps:

[0034] Wheat (Yannong No. 19) seeds were evenly placed on 4 sponges 2 cm thick, with a 1 cm interval between adjacent seeds. Covered with a double layer of gauze, placed in 4 water tanks (30×10×10cm), of which 2 water tanks had 1L of distilled water as the culture medium, and the other two water tanks had 1L of tap water as the culture medium. Two stainless steel plates (10×10×0.1cm) were inserted in parallel at both ends of each water tank, and then connected to the anode and cathode of the electrophoresis instrument respectively. The water tank voltage was controlled by the electrophoresis instrument, and a 10V voltage was applied to each tap water and distilled water tank, and the remaining treatment group was not applied with voltage as a control. After 6 days of dark culture at 20±0.1℃, root samples were collected for metabolomics detection. The experiment was repeated 6 times, that is, 6 batches of samples were collected.

[0035] Metabolomics Refer to Table 1 below. When 10V voltage and 0.005mA current were applied to wheat, the content of pyruvate, an important metabolite in the glycolysis pathway of the root system, decreased compared with the control group, indicating that the metabolic process of the glycolysis pathway was weakened; the content of malic acid, an important metabolite in the tricarboxylic acid cycle, increased compared with the control group, indicating that the metabolic process of the tricarboxylic acid cycle was enhanced;

[0036] When 10V voltage and 2mA current were applied to wheat, the content of pyruvate, an important metabolite in the glycolysis pathway of the root system, increased compared with the control group, indicating that the metabolic process of the glycolysis pathway was enhanced; the content of malic acid, an important metabolite in the tricarboxylic acid cycle, increased compared with the control group, indicating that the metabolic process of the tricarboxylic acid cycle was enhanced;

[0037] The above results indicate that the biologically presented electro-bitropic properties can be used to regulate the energy metabolism of plants in a targeted manner.

[0038] Table 1 Effects on glycolysis pathway and tricarboxylic acid cycle under 10V voltage, 0.005mA current and 2mA current conditions

[0039]

[0040] Although the specific implementation modes of the present invention are described above, those skilled in the art should understand that the specific implementation modes described are only illustrative and are not intended to limit the scope of the present invention. Any modifications and changes made by those skilled in the art in accordance with the spirit of the present invention should be included in the scope of protection of the claims of the present invention.

Claims

1. A method for regulating plant energy metabolism, characterized in that: Utilizing plant electroditropism to regulate plant energy metabolism.

2. A method for regulating plant energy metabolism according to claim 1, characterized in that: The energy metabolism of plants can be regulated by regulating the tendency of plant roots toward the cathode and anode.

3. A method for regulating plant energy metabolism according to claim 2, characterized in that: The plants are cultured in a closed loop system with a culture matrix, and the voltage and current are adjusted to determine the voltage and current ranges when the plant roots tend toward the cathode and anode respectively; the root electrotropism is regulated according to the determined voltage and current ranges, thereby achieving directional control of plant energy metabolism.

4. A method for regulating plant energy metabolism according to claim 3, characterized in that: S1, insert two metal plates in parallel on both sides of a rectangular water tank, the two metal plates are respectively connected to the positive electrode and the negative electrode of a DC power supply, add a culture matrix into the water tank to form a closed loop, place seeds on the sponge and cover it with a double layer of gauze, and then place the sponge into the culture matrix of the water tank to culture the seeds; S2, by adjusting the voltage intensity applied to both sides of the culture matrix and the current intensity flowing through the culture matrix, determine the voltage and current ranges when the plant roots tend toward the cathode and the anode respectively; S3, based on demand, uses the voltage and current of the plant root system to exhibit anode or cathode tropism to systematically regulate plant energy metabolism.

5. Use of the method according to any one of claims 1 to 4, characterized in that: Applications in biology and crop science.

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