Strawberry stolon guiding and inserting seedling raising method
By combining the parent plant cultivation device and the stolon lead-in device, using special nutrient solution and spray irrigation, combined with inclined hole plates and fill lights, the problems of difficulty in inducing stolon production and low seedling efficiency in the existing strawberry stolon traction and reproduction methods are solved, and the efficient generation and survival of strawberry stolons are achieved, and the quality and production efficiency of commercial seedlings are improved.
Patent Information
- Application Number
- CN202510229590.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2025-05-13
- Estimated Expiration
- 2045-02-28
AI Technical Summary
The existing strawberry stolon traction reproduction methods have problems such as difficulty in inducing stolon production, low seedling efficiency, uneven seedling age, aging root system and high pest risks, and are also inefficient in production management and high labor costs.
A strawberry stolon seedling cultivation method is adopted, combined with the mother plant cultivation device and the stolon seedling device, and the special mother plant nutrient solution and spray irrigation device are used, and the tilted hole plate and fill light are combined to promote the generation and survival of strawberry stolon.
It significantly improves the number of strawberry stolon production and survival rate, shortens the seedling cultivation cycle, improves the quality and production efficiency of commercial seedlings, and reduces labor costs and pest risks.
Smart Images

Figure CN119969207A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of strawberry seedling cultivation, and in particular to a strawberry runner-inserting seedling cultivation method. Background Art
[0002] Common methods for raising strawberry seedlings include seeds, division, runner traction propagation, tissue culture, etc., and the most commonly used method in production is runner traction propagation. Runners refer to stems that grow horizontally. The internodes of the side branches at the base are long, and leaves, buds and adventitious roots can grow on each node. After being separated from the whole, they can grow into new individuals, so they can be used for artificial vegetative propagation. Runners generally have slender internodes, with buds and adventitious roots between the nodes, and the buds will grow into new plants later.
[0003] However, the current method of propagation by traction of runners has certain drawbacks. First, the production of strawberry runners usually requires a certain induction treatment of the mother plant to accelerate the production of runners and increase the number of runners. The method usually adopted is to select strawberry varieties that are easy to produce runners and erect the strawberry mother plants at high places. Therefore, it is difficult to use this seedling method for some varieties, and the method of inducing the production of runners is single; second, the seedling efficiency is low, and the introduction and insertion of runners requires a certain space, but the introduction and insertion can only be within the length range of the runners. Therefore, the commercial seedlings that can be produced by traction propagation of runners for each mother plant are limited; third, there is the problem of the variety of runner seedlings. At all levels, The age of the runner seedlings is uneven. After the first-level runners are pulled and rooted, the time from planting is generally about 3 months, resulting in a long seedling age, serious root aging, and black root color. The black root rots and breeds bacteria, and there is a risk of anthracnose and blight. After planting, there may also be problems such as uneven or slow flower bud differentiation. In addition, the production operation of traction seedlings is relatively scattered, the production management efficiency is low, and the labor cost is high. At the same time, the current traction seedlings of runner substrates have large root substrate blocks, which is costly in the long-distance transportation of commercial seedlings. If the substrate is removed, there are risks and problems of survival rate and slow seedlings during planting. At present, there have been reports on the application of strawberry cutting seedlings in production, but there is a lack of unified standards for the seedling retrieval time, seedling retrieval technology, and cutting technology. The survival rate and seedling quality of cuttings are not high and unstable, making it difficult to achieve intensive and efficient strawberry seedlings.
[0004] Patent CN202110381405.4 discloses an application and promotion method of yellow-green mixed light quality in promoting the production of strawberry runners. It proposes that using a certain proportion of yellow-green mixed light quality to illuminate the strawberry mother plant can promote the production of strawberry runners. However, there is no detailed study on the maximum number of strawberry runners produced, the survival rate after seedling cultivation, and the seedling quality.
[0005] Therefore, further research is urgently needed on the strawberry runner traction propagation method in order to find a strawberry runner seedling method that simultaneously promotes the production of strawberry runners, increases the maximum number of strawberry runners produced, and improves the survival rate and seedling quality after seedling cultivation. Summary of the invention
[0006] In order to solve the above-mentioned existing problems, the present invention discloses a strawberry runner introductory seedling raising method, which combines a variety of new runner introductory seedling raising technologies that can produce synergistic effects, while promoting the production of strawberry runners, increasing the maximum number of strawberry runners produced, and improving the survival rate and seedling quality after introductory seedling raising.
[0007] On the one hand, the present invention provides a strawberry runner insertion and seedling raising method, which is carried out using a strawberry runner insertion and seedling raising device, the strawberry runner insertion and seedling raising device comprising a mother plant cultivation device and a runner insertion device, the mother plant cultivation device is planted with a strawberry mother plant, the strawberry mother plant produces runners, and the runner insertion device comprises a plug tray for receiving the runners, and the plug tray is placed at an angle.
[0008] It is noteworthy that the present invention can simultaneously take into account the fruit production of the mother plant while carrying out the introduction of runners, and actually carries out the production of strawberry fruits and the production of commercial strawberry seedlings at the same time.
[0009] Preferably, the inclination angle of the plug tray is 30-70°; the inclined placement of the plug tray can fully utilize the space to increase the number of strawberry runners per unit area for seedling cultivation, and the inclination can also accelerate the rooting of the seedlings.
[0010] Furthermore, the mother plant cultivation device also includes a substrate and a tidal irrigation device, the substrate is used to plant strawberry mother plants, and the tidal irrigation device is used to periodically provide water or mother plant nutrient solution to the strawberry mother plants.
[0011] The use of tidal irrigation devices for irrigation water and mother plant nutrient solution can automatically irrigate strawberry mother plants planted in the same row in batches. At the same time, the spatial density of water and nutrients in the matrix can be controlled to be uniform. By controlling the temperature of water or mother plant nutrient solution, the temperature of the strawberry mother plant root system can also be controlled, thereby maintaining stable conditions that promote the production of runners by the strawberry mother plants.
[0012] In some embodiments, the temperature of the water or mother plant nutrient solution is 15-25°C.
[0013] The present invention finds that controlling the temperature of the strawberry mother plant root system at 15-25°C is more conducive to the production of runners by the strawberry mother plant. For example, in summer, well water or nutrient solution prepared with well water can be used for irrigation. The method of using the temperature of water or the mother plant nutrient solution to regulate the root system temperature is convenient and the temperature control is stable.
[0014] In some embodiments, the mother plant nutrient solution includes a high-nitrogen nutrient solution.
[0015] The high-nitrogen nutrient solution is a nutrient solution containing high-concentration nitrogen, which can provide abundant nitrogen for strawberry mother plants. For example, the present invention uses a relatively high-concentration ammonium nitrate solution as the mother plant nutrient solution, and other nitrate, ammonium salt and urea solutions can also be used as a high-nitrogen nutrient solution.
[0016] It is worth noting that one of the purposes of the present invention is to accelerate the production of runners. The reason is that when the means for promoting the production of runners are taken, when the first runner is produced, a large number of runners will soon be produced; in the absence of intervention, the first runner is produced later and the maximum number of runners is smaller; therefore, there is a certain correlation between the time of appearance and the maximum number of runners. The earlier the runners are produced after intervention, the greater the number of runners in the end, which is conducive to the large-scale production of commercial strawberry seedlings.
[0017] In some embodiments, the mother plant nutrient solution includes chelated iron, calcium nitrate and 6-BA.
[0018] The mother plant nutrient solution of the present invention is a nutrient solution specially prepared for promoting the production of runners by strawberry varieties that are not easy to produce runners, wherein the chelated iron is EDTA-Fe, which is usually used to promote chlorophyll synthesis, calcium nitrate mainly provides nitrogen and calcium elements, nitrogen is an essential element for plants, calcium is usually used to strengthen cell walls to prevent diseases, and 6-BA is chemically called 6-benzylaminopurine, which is often used to cause plant growth and development by stimulating cell division; the present invention adopts high-nitrogen nutrient solution in the early stage of research, which has a certain effect, but it is not effective for strawberry varieties that are not easy to produce runners. The strawberry strain has a relatively low effect in promoting the production of runners, so the inventors continued their research and found that the nutrient solution containing the above three substances can significantly promote the production of runners in strawberry strains that are not easy to produce runners, mainly manifested in shortening the time of runner production; further research found that it is actually the nitrogen, calcium, chelated iron and 6-BA that can play a synergistic effect. When any one of the substances is missing or its content is reduced, the efficacy is significantly reduced. Therefore, the inventors speculate that the principle of action is that the above substances are all necessary substances in a physiological pathway that promotes the production of runners.
[0019] Furthermore, the mother plant cultivation device also includes a fill light for irradiating upwards.
[0020] The present invention finds that the upward irradiation of the supplementary light has a beneficial effect on the production of strawberry runners. The reasons may be that, firstly, the supplementary light is arranged on the surface of the substrate, close to the strawberry leaves, so the light loss rate is low and the supplementary light efficiency is higher. Secondly, when the light is usually irradiated from top to bottom, the lighting of the bottom leaves will be blocked by the top leaves, and the bottom leaves are prone to aging due to insufficient lighting. However, the supplementary light from bottom to top of the present invention can activate the physiological function of the bottom leaves, which has a significant beneficial effect on the growth and development of the mother plant and the production of runners. Finally, the physiological processes carried out on the two sides of the leaf when illuminated are different. The present invention finds that supplementary light on the back of the leaf is beneficial to the production of runners. In addition, the specific form of the supplementary light is a long strip of supplementary light laid on the substrate, which can provide supplementary light to the same row of strawberry mother plants at the same time, while saving the upper space and increasing the number of strawberry seedlings per unit space.
[0021] In some embodiments, the supplemental light provides 14-16 hours of light per day.
[0022] Prolonging the light intensity is related to the physiological function of strawberries. Usually, under the light intensity of 12 hours per day, strawberries tend to differentiate flower buds and bloom, and tend not to produce runners. The present invention finds that under the light intensity of 14-16 hours per day, strawberries are more inclined to produce runners.
[0023] In some embodiments, the light quality provided by the fill light is red light: purple light = 1:3-10.
[0024] The light quality in the present invention is generally understood as follows: red light is light with a wavelength of 640-780 nm, and violet light is light with a wavelength of 380-450 nm.
[0025] Although current studies have pointed out that a certain mixed light quality is conducive to the production of runners, the principle is that specific wavelengths can cause specific physiological processes in strawberry plants, but the method adopted by the present invention is to combine irrigation with specific nutrient solutions and seedling cultivation. It is understandable that promoting the production of runners is not limited to a single physiological pathway. Therefore, for the present invention, further research is needed to find light qualities that can have a synergistic effect with irrigation with specific nutrient solutions.
[0026] The present invention has found through experiments that, based on the above-mentioned mother plant nutrient solution containing chelated iron, calcium nitrate and 6-BA, the additional addition of red and blue mixed light can significantly accelerate the production of runners. The principle may be that a certain light quality can promote the activation of corresponding physiological pathways, and the wavelength of light and the mixing ratio are more critical when cells receive light. The present invention has found that only the above-mentioned mixed light quality can have an effect significantly exceeding other light qualities.
[0027] Furthermore, the runner insertion device includes a spray irrigation device, which is used to periodically provide water or runner nutrient solution to the runners.
[0028] The advantage of spray irrigation is that, on the one hand, it evenly provides water and nutrient solution to the surface of the runners to stimulate the root system of the runners; on the other hand, because the spray point is set above the inclined plug tray, the amount of water and nutrient solution received by the runners decreases with the increasing distance from the mother plant. Compared with uniform irrigation of runners at all levels, experiments have shown that this method is more conducive to improving the quality of runner seedlings.
[0029] In some embodiments, the runner nutrient solution includes a high phosphorus nutrient solution.
[0030] The high-phosphorus nutrient solution is a nutrient solution containing high-concentration phosphorus, which can provide abundant phosphorus for strawberry mother plants. For example, the present invention selects a solution of potassium dihydrogen phosphate as the high-phosphorus nutrient solution, and solutions of other phosphates, phosphorus oxides, etc. can also be used as a high-phosphorus nutrient solution.
[0031] In some embodiments, the stolon nutrient solution includes potassium dihydrogen phosphate, indolebutyric acid, and lysine.
[0032] The stolon nutrient solution of the present invention is a nutrient solution specially prepared for promoting the rooting of stolons, wherein potassium dihydrogen phosphate provides phosphorus and potassium elements, indolebutyric acid is often used for cell elongation and root production, and lysine is often used for improving the stress resistance of plants; the initial study of the present invention used a high-phosphorus nutrient solution for spray irrigation, which had a good effect on seedling quality, but the survival rate was low, and the survival rate was the probability of survival of seedlings obtained by seedling transplanting after planting; the inventors further studied and found that spray irrigation with a nutrient solution containing potassium dihydrogen phosphate, indolebutyric acid and lysine can significantly improve the survival rate of stolons; in fact, the inventors conducted a large number of formula experiments and finally obtained a streamlined formula composed of three substances, because the above three substances have a synergistic effect, and when any one of the substances is lacking, the survival rate is significantly reduced.
[0033] It is worth noting that the application of the above-mentioned runner nutrient solution is best carried out in conjunction with the mother plant nutrient solution and mixed light quality. The reason is that the state of the runners is determined by the mother plant. Therefore, the runner nutrient solution must be reasonably configured to achieve the corresponding effect only when the mother plant's nutrient solution and cultivation method are clear.
[0034] Furthermore, in the stolon insertion device, one mother plant cultivation device corresponds to multiple stolon insertion devices.
[0035] For strawberry varieties that are prone to producing runners, a strawberry mother plant can usually produce about 20 runners. Therefore, multiple runners produced in a mother plant cultivation device require multiple runner introduction and insertion devices to correspond.
[0036] Furthermore, the strawberry runner insertion and seedling raising device also includes a first platform, a second platform and a bracket. The first platform and the second platform are used to fix the mother plant cultivation device and the runner insertion device, respectively. The first platform and the second platform are both arranged on the bracket and are both height-adjustable.
[0037] The plug tray of the invention is placed on an inclined pad, which is supported by a second platform. The number of plug trays can be reduced or increased by lifting the second platform, and dynamic adjustment can be performed when runners of various levels of strawberries are produced.
[0038] In summary, the present invention has the following beneficial technical effects: The invention discloses a new strawberry runner introductory seedling raising method, which combines a plurality of new runner introductory seedling raising technologies capable of producing synergistic effects, and simultaneously promotes the production of strawberry runners, increases the maximum number of strawberry runners produced, and improves the survival rate and seedling quality after introductory seedling raising. BRIEF DESCRIPTION OF THE DRAWINGS
[0039] Figure 1 : A schematic diagram of the structure of a strawberry runner-inserting seedling raising device; Figure numerals: 1-strawberry mother plant, 2-runner, 3-substrate, 4-mother plant cultivation container, 5-tidal irrigation device, 6-fill light, 7-hole tray, 8-pad, 9-first platform, 10-second platform, 11-bracket, 12-clip, 13-spray irrigation device. DETAILED DESCRIPTION
[0040] The present invention is further described in detail below in conjunction with the accompanying drawings and specific examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention. The materials, reagents, etc. used in the following examples are reagents and materials available from commercial sources unless otherwise specified.
[0041] Embodiment 1: Strawberry runners inserting seedling raising method The strawberry runner introductory seedling raising method of the present invention is carried out by means of a strawberry runner introductory seedling raising device. The structural schematic diagram of the strawberry runner introductory seedling raising device is shown in FIG. Figure 1The strawberry mother plant 1 is planted in a mother plant cultivation container 4, the mother plant cultivation container 4 is filled with a matrix 3, the tidal irrigation device 5 contains a mother plant nutrient solution, the bottom of the mother plant cultivation container 4 is provided with a plurality of micropores for allowing the mother plant nutrient solution in the tidal irrigation device 5 to penetrate into the matrix 3, the surface of the matrix 3 is provided with a fill light 6, the fill light 6 is used for illuminating upward, the strawberry mother plant 1 will produce runners 2, the roots produced by the runners 2 fall into a plurality of inclined plug trays 7 in sequence, and each plug tray 7 is placed and fixed on the inclined plug tray 7. The first platform 10 is provided with a pad 8, one side of the pad 8 is provided on the second platform 10 and forms an angle of 60°, the second platform 10 is connected and height-adjusted with the bracket 11 through a buckle 12, and the height adjustment of the first platform 10 will also affect the pad 8, thereby adjusting the position and number of the plug tray 7, the mother plant cultivation container 4 is provided on the first platform 9, and a spray irrigation device 13 is also provided on the first platform 9, the spray irrigation device 13 is located above the plug tray 7, and the spray irrigation device 13 atomizes the stolon nutrient solution and sprays it downward.
[0042] Figure 1 What is shown is a cross-sectional view of a strawberry runner-inserting seedling raising device. In fact, when raising seedlings, the strawberry runner-inserting seedling raising device is arranged in a vertical row along a direction perpendicular to the paper surface. The mother plant cultivation container 4, the tidal irrigation device 5, the fill light 6 and the spray irrigation device 13 are all extended along a direction perpendicular to the paper surface to play a role in batch seedling raising; the tidal irrigation device 5 is a strip-shaped nutrient solution bag arranged in the mother plant cultivation container 4, the fill light 6 is a light strip with a waterproof function, and is also provided with a variety of different LED units to adjust the generation and mixing ratio of different light qualities. The fill light 6 has a control panel to facilitate the adjustment of the mixed light quality. The spray irrigation device 13 is a tubular container, and an atomizer is arranged above each group of hole trays 7 to perform spray irrigation above each group of hole trays 7.
[0043] The following is a detailed method for using the strawberry runner introductory seedling raising device to carry out strawberry runner introductory seedling raising: First, the strawberry mother plant 1 and the matrix 3 are moved as a whole into the mother plant cultivation container 4. In this embodiment, the Akihime strawberry that is easy to produce runners is selected as the test variety. At this time, the tidal irrigation device 5 and the fill light 6 are turned on at the same time. The production of strawberry runners is mainly concentrated in the summer when the temperature is higher. The experiment started in May 2023. Well water with a temperature of 15-25°C is used as a solvent to prepare the mother plant nutrient solution in the tidal irrigation device 5. The specific composition is 10 mg / L chelated iron, 500 mg / L calcium nitrate and 0.5 mg / L 6-BA. A sufficient amount is configured at one time. A water pump with a set program is used to irrigate once every 15 minutes, and each irrigation time is 5 minutes; the fill light 6 is set to a mixing ratio of red light and purple light of 1:5.5, the light intensity is set to 8000 Lux, and the light is 15 hours / day; After 12 days of the above process, the first runner is produced, and the produced runner is inserted into the plug tray 7, and then multiple runners are quickly produced. Here, only the first runner is discussed as an example; at this time, the spray irrigation device 13 is turned on, and the runner nutrient solution in the spray irrigation device 13 contains 150 mg / L potassium dihydrogen phosphate, 150 mg / L indolebutyric acid and 1 mg / L lysine. The spraying time is to turn on the spray every 1 hour between 8 am and 16 pm every day, and turn off the spray when the leaf surface is thoroughly wet, so as to ensure that the plug tray is kept moist and rich in nutrient solution at all times; Six seedlings are inserted into each runner. When the last seedling takes root, the plug tray is removed and a new set of plug trays can be replaced to continue the insertion. After 20 days, the six plug trays 7 set on each pad 8 have completed the insertion and seedling cultivation of the runners, and each runner produces 6 strawberry seedlings. The above is only the insertion and seedling cultivation of the first runner produced by each strawberry mother plant. In fact, Zhangji strawberry is a variety that is easy to produce runners. In the summer of 2023, a total of 5 Zhangji strawberry mother plants were used for the above-mentioned insertion and seedling cultivation, and each strawberry mother plant produced an average of 6 strawberry seedlings. 20.8 runners were grown, and 620 strawberry seedlings were planted and survived in 624 plug trays for insertion, with a survival rate of 99.4%. The survival rate of conventional runner insertion seedlings is about 80%, which shows that the method of the present invention significantly improves the survival rate of Akihime strawberry. Further cultivation shows that the commercial seedlings obtained by the present invention are of excellent quality, as reflected in rapid growth and few diseases and insect pests. In addition, while inserting and raising seedlings by runners, the present invention can also provide sufficient nutrition for the strawberry mother plants to promote their fruiting, thereby significantly improving the production efficiency.
[0044] Example 2: Effects of different irrigation methods on seedling cultivation The irrigation of the present invention is tidal irrigation for the mother plant and spray irrigation for the runners. This is the preferred method obtained through experiments in the preliminary research of the present invention. This embodiment lists cases of adopting other irrigation methods.
[0045] For mother plants, in addition to tidal irrigation, previous studies also used over-irrigation and spray irrigation. The final difference lies mainly in the time of runner production; the irrigation is carried out at 6 am and 8 pm every day, 300 mL / time per plant, and the mother plant nutrient solution in Example 1 is irrigated; the spray irrigation is carried out at 8 am and 16 pm every day, spraying the mother plant nutrient solution in Example 1, and the spraying is stopped when the leaf surface is thoroughly wet; the strawberry variety is Zhangji strawberry. The first runner of the strawberry mother plant using tidal irrigation takes 12 days to produce, the first runner of the strawberry mother plant using over-irrigation takes 17 days to produce, and the first runner of the strawberry mother plant using spray irrigation takes 18 days to produce.
[0046] For runners, in addition to spray irrigation, drip irrigation was also used in the previous study. The strawberry mother plants of the two were cultivated according to the method of Example 1. The spray irrigation was the same as in Example 1, and the drip irrigation was drip-irrigated with the runner nutrient solution of Example 1 to the plug tray for inserting the runners. A certain drip irrigation frequency was set, with high frequency during the day and low frequency at night. Each plug tray was drip-irrigated with 100 mL / day in total. The final difference was mainly in the rooting time of the last seedling. The last seedling of the spray irrigation took root 20 days after the experiment, and the last seedling of the drip irrigation took root 23 days after the experiment.
[0047] Therefore, different irrigation methods affect the efficiency of strawberry seedling raising. Tidal irrigation for mother plants and spray irrigation for runners are preferred. The principle may be that tidal irrigation can timely regulate the temperature of the substrate and replenish the nutrients in the substrate in time, thereby continuously inducing the production of runners. Spray irrigation can also regulate the overall temperature of the runner area, increase humidity, and replenish the nutrients needed for rooting in time.
[0048] Example 3: Effect of the placement of the plug tray on seedling cultivation The present invention adopts a method of placing the plug tray at an angle, which is a preferred method verified by experiments. Compared with the conventional flat placement, the inclined placement of the plug tray can save space and improve the seedling raising efficiency.
[0049] The plug tray is placed flat, that is, each plug tray is placed on a plane flush with the cultivation container of the strawberry mother plant. At this time, the spray irrigation device is directly set 30 cm above the plug tray. Other conditions are the same as in Example 1. The last seedling takes root 22 days after the experiment, which is later than the experimental group with the plug tray placed tilted.
[0050] The possible principle is that the tilted placement of the plug tray makes the total amount of nutrient solution sprayed on the runners different, and the amount of nutrient solution applied has a certain effect on the rooting of the seedlings.
[0051] Further experiments showed that the optimal angle for the plug tray to be tilted is 30-70°, which results in faster rooting of the seedlings.
[0052] Example 4: Effect of the composition of the nutrient solution of the mother plant on seedling cultivation Generally speaking, in order to promote the growth of runners on the mother plant, high-nitrogen nutrient solution should be applied. In preliminary experiments, the use of high-nitrogen nutrient solution for Akihime strawberry achieved good results. However, it was also noted that strawberry varieties that are not easy to produce runners, such as four-season strawberry, still find it difficult to produce runners.
[0053] This embodiment configures a series of mother plant nutrient solutions to perform tidal irrigation on four-season strawberries. Except for the difference in the composition of the mother plant nutrient solutions, all other aspects are carried out with reference to Example 1. The difference in the efficacy of different mother plant nutrient solutions in promoting the growth of four-season strawberry mother plants is determined according to the time when the runners are produced. Five groups are set up in parallel, and the mean of each group is calculated. Some experimental results are shown in Table 1.
[0054] Table 1: Differences in the efficacy of different mother plant nutrient solutions in promoting the growth of runners in mother plants (n=5)
[0055] According to the above experimental results, the specific composition of the mother plant nutrient solution has an impact on the introduction and seedling raising of strawberry varieties that are not easy to produce runners, because of the difference in the efficacy of promoting the production of runners; high-nitrogen nutrient solution usually prepared with ammonium nitrate was found to produce primary runners until 33 days; the mother plant nutrient solution preferably containing chelated iron, calcium nitrate and 6-BA in the present invention can shorten the runner production time to 18.2 days, significantly shortening the seedling raising cycle; when one of the reagents is removed or calcium nitrate is replaced by potassium nitrate, the runner production time is significantly increased, which shows that there is a synergistic effect between chelated iron, nitrogen, calcium and 6-BA, and its mechanism of action may be that a physiological pathway that promotes the production of runners requires chelated iron, nitrogen, calcium and 6-BA.
[0056] Example 5: Effect of supplementary lighting on seedling cultivation The usual supplementary lighting is to supplement the light above the strawberry mother plant, irradiating white visible light with a light intensity of 5000-10000 Lux. This method can improve the physiological activity of the top leaves of the strawberry, which is conducive to promoting fruiting, but has no obvious effect on the production of runners, and even has a negative effect on the production of runners of some varieties. The present invention has found in the research that setting the supplementary light source at the bottom of the strawberry mother plant, that is, irradiating upward from the bottom of the leaves, can promote the production of runners, as follows: The supplementary lighting from above is performed by setting a supplementary lighting lamp 30 cm above the strawberry mother plant, and the light intensity is 8000 Lux, and the rest is the same as in Example 1; the supplementary lighting from below is performed strictly in accordance with the method of Example 1; 20 groups are set up in parallel, and the average of the runner production time of all strawberry mother plants receiving supplementary lighting from above is 14.4 days, which is 11.8 days later than the runner production time of the strawberry mother plants receiving supplementary lighting from below.
[0057] Regarding light intensity, the present invention conducted a series of experiments and found that when the light intensity was 5000-10000 Lux, the time for the runners of the Akihime strawberry mother plants to produce was not much different, all about 12 days; among them, the preferred light intensity was 8000 Lux, which could produce runners for some strawberry mother plants one day earlier.
[0058] Regarding the selection of light quality and the mixing ratio, the present invention conducted a series of experiments, setting up 10 groups in parallel, and the method of introducing and planting seedlings was the same as in Example 1, the only difference being the mixed light quality. The mixed light quality can be directly modified by adjusting the parameters of the fill light panel. Some of the experimental results are shown in Table 2.
[0059] Table 2: Differences in the efficacy of different mixed light qualities in promoting the growth of runners in mother plants (n=10)
[0060] According to the above experimental results, the selection of light quality has a significant effect on the production of runners. The best light quality is a mixture of red light and purple light, with a mixing ratio of 1:3-10, and the most preferred is 1:5.5; although red light accounts for a small proportion, if only purple light is used for irradiation, the production time of runners will be significantly prolonged, indicating that there is a synergistic effect between red light and purple light; when purple light is replaced by blue light, the production time of runners will also be significantly prolonged, indicating that the wavelength range is the core influencing factor; using the existing publicly available yellow-green mixed light quality, it was found that there was no significant promoting effect on the production of runners. The reason may be that the direction of light is from bottom to top, involving completely different physiological mechanisms, and therefore cannot be directly migrated and used.
[0061] Regarding the supplementary lighting time, the present invention found that extending the supplementary lighting time to 14-16 hours / day can promote the production of runners.
[0062] Further research found that the effectiveness of mixed light quality is correlated with the composition of the mother plant nutrient solution. When high-nitrogen nutrient solution prepared with ammonium nitrate is used directly to cultivate four-season strawberries that are not easy to produce runners, it is difficult to shorten the time of runner production even if supplementary lighting is performed according to the above-mentioned preferred method. This suggests that the composition of the nutrient solution and supplementary lighting may act on the same physiological pathway and need to be combined for seedling cultivation to achieve the best effect.
[0063] Example 6: Effect of stolon nutrient solution on seedling cultivation Generally speaking, in order to promote the root growth of runners, a high-phosphorus nutrient solution should be applied. For strawberry varieties that are prone to produce runners, applying any reasonable nutrient solution will not bring significant differences; however, the growth of runner roots into seedlings is correlated with the growth of runners from the mother plant, especially for strawberry varieties that are difficult to produce runners. The present invention uses mixed light quality supplementary lighting and tidal irrigation with mother plant nutrient solution to synergistically promote the production of runners, which is very likely to be detrimental to the root growth and seedling formation of runners. Therefore, when promoting the growth of runner roots and the formation of commercial seedlings, it is necessary to develop a new runner nutrient solution.
[0064] The present embodiment is configured with a series of runner nutrient solutions, and spray irrigation is performed on the runners produced by the four-season strawberry mother plant. Except for the difference in the components of the runner nutrient solution, all other methods are performed with reference to Example 1. Each group of runner nutrient solutions is tested 5 times in parallel. The four-season strawberry mother plant produces 5-10 runners. According to the growth of the runners, 6 seedlings are introduced and grown for each runner. The survival rate (number of surviving seedlings / total number of introduced and grown seedlings) after final planting is calculated. Some experimental results are shown in Table 3.
[0065] Table 3: The effect of different runners on survival rate
[0066] According to the above experimental results, when inducing the growth of runners for strawberry varieties that are not easy to produce runners, the influence of different configurations of runner nutrient solutions on the survival rate is relatively large. It is preferred to use a runner nutrient solution containing potassium dihydrogen phosphate, indolebutyric acid, and lysine for spray irrigation. When any one of the components is reduced, the final survival rate will be significantly reduced.
[0067] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto, and any equivalent modifications or substitutions that can be easily thought of by any technician familiar with the technical field within the technical scope disclosed by the present invention should be included in the protection scope of the present invention.
Claims
1. A strawberry runner seedling raising method, characterized in that: The method is carried out using a strawberry runner seedling raising device, which includes a mother plant raising device and a runner raising device. The mother plant raising device is planted with a strawberry mother plant that produces runners. The runner raising device includes a plug tray for receiving the runners, and the plug tray is placed at an angle.
2. A strawberry runner seedling raising method as claimed in claim 1, characterized in that: The mother plant cultivation device also includes a substrate and a tidal irrigation device, wherein the substrate is used to plant strawberry mother plants, and the tidal irrigation device is used to periodically provide water or mother plant nutrient solution to the strawberry mother plants.
3. A strawberry runner seedling raising method as claimed in claim 2, characterized in that: The temperature of the water or mother plant nutrient solution is 15-25°C.
4. A strawberry runner seedling raising method as claimed in claim 2, characterized in that: The mother plant nutrient solution includes a high-nitrogen nutrient solution.
5. A strawberry runner seedling raising method as claimed in claim 1, characterized in that: The mother plant cultivation device also includes a supplementary light for irradiating upwards.
6. A strawberry runner seedling raising method as claimed in claim 5, characterized in that: The supplementary light provides 14-16 hours of light per day.
7. A strawberry runner seedling raising method as claimed in claim 1, characterized in that: The runner inserting device comprises a spray irrigation device, and the spray irrigation device is used to periodically provide water or runner nutrient solution to the runners.
8. A strawberry runner seedling raising method as claimed in claim 7, characterized in that: The runner nutrient solution includes a high-phosphorus nutrient solution.
9. A strawberry runner seedling raising method as claimed in claim 1, characterized in that: In the stolon insertion device, one mother plant cultivation device corresponds to a plurality of stolon insertion devices.
10. A strawberry runner seedling raising method as claimed in claim 1, characterized in that: The strawberry runner insertion and seedling raising device also includes a first platform, a second platform and a bracket. The first platform and the second platform are respectively used to fix the mother plant cultivation device and the runner insertion device. The first platform and the second platform are both arranged on the bracket and are both height-adjustable.
Citation Information
Patent Citations
Use of yellow-green mixed light quality in promoting generation of strawberry stolons and promotion method
CN112956378A
Strawberry cultivating device and method
CN101971761A
Fast growth technique of strawberry seedlings
CN103563591A
Elevated seedling raising device for strawberry
CN109392687A
Strawberry elevated propagation plug cutting seedling raising method
CN115735586A