Artificial breeding technology for pyemotes moschus under different temperature conditions

Abstract

The invention discloses an artificial breeding technology of the pyemotes molasses under different temperature conditions, according to the artificial breeding technology, Dioryctria syrvestris, Dioryctria abietella, Helicoverpa armigera Hubner and Ostrinia furnacalis in lepidoptera larvae are selected as alternative hosts of the pyemotes molasses, an anti-escape mite receiving box is used for receiving the pyemotes molasses, meanwhile, the breeding temperature conditions are regulated and controlled, and the pyemotes molasses are bred.

Description

Technical Field

[0001] The invention relates to the technical field of biological pest control, and in particular to an artificial breeding technology of Moser mites under different temperature conditions. Background Art

[0002] Pyemotes are external parasites of many insects. They are important organisms and important biological control factors for social and hidden pests. They have broad application prospects. Pyemotes are distributed all over the world and widely parasitize the larvae and pupae of Lepidoptera, Coleoptera and Hymenoptera insects. They are considered to have the potential of biological control methods. Application of Pyemotes in pest control: Pyemotes are small in size, have short generations, large populations and strong reproductive capacity, and are easy to raise artificially. Moser Pyemotes (Pyemotes moseri) has a high parasitism rate, has a strong ability to attack and search for hosts, and has many excellent characteristics. It is an ideal natural enemy of stem borers.

[0003] At present, breeding natural enemies of Pleurotus erythrocytes still relies on alternative hosts. When breeding Pleurotus erythrocytes artificially, temperature is the main factor. The present invention sets different temperatures to select alternative hosts of Pleurotus erythrocytes at different temperatures. Summary of the invention

[0004] The invention aims to provide an artificial breeding technology of Moser's Pleurotus erythroxylum under different temperature conditions.

[0005] In order to achieve the above object, the present invention proposes the following technical solution: providing an artificial breeding technology of Moser mites under different temperature conditions, characterized in that it includes the following steps:

[0006] (1) The first generation of artificially bred Moser mites: The Moser mites with wild characteristics were collected from the wild, placed in a culture dish at a temperature of 25±1°C and a relative humidity of 50±5%, and cultured for about 7 days. After the mites had young mites, healthy honey bee larvae were placed in a mite storage tube, and then placed in a culture dish with young mites. When all the young mites developed into mature mites, the mites in the storage tube were used as the first generation of artificial mites.

[0007] (2) Preparation of alternative hosts for Moser's mites: The larvae of red pine moth, fir moth, cotton bollworm and Asian corn borer are used as alternative hosts. The alternative hosts and the first generation of artificially bred Moser's mites are placed in a mite inoculation box at 16-25°C and relative humidity of 45-60%. The ratio of the alternative hosts to the first generation of artificially bred Moser's mites is 1:3. The boxes are placed in an incubator for 6-14 days to obtain mature, expanded abdominal bodies of the alternative hosts for Moser's mites.

[0008] (3) Transfer of the inflated bodies of Moser's mites: Take out the larvae of red pine moth, fir moth, cotton bollworm and Asian corn borer that have been inoculated with mature inflated bodies of Moser's mites from the mite inoculation box and put them into mite storage tubes respectively. Then stick a circle of double-sided tape on the outside of the mite storage tube or plug the mouth of the mite storage tube with absorbent cotton. Put it in an incubator and wait for the mature inflated bodies to breed offspring mites. DETAILED DESCRIPTION

[0009] The following examples further illustrate the present invention, but should not be construed as limiting the present invention.

[0010] (1) The first generation of artificially bred Moser mites. When a large number of Moser mites have emerged, they are placed in a mite inoculation box with a ratio of 1:3 with the replacement hosts, and a control group without mite inoculation is set up.

[0011] (2) Preparation of alternative hosts for Moser's mites: The larvae of red pine moth, fir moth, cotton bollworm and Asian corn borer were used as alternative hosts. The larvae were cultured in an incubator at 15-18℃, 19-22℃ and 23-26℃, with a relative humidity of 50%. The ratio of the number of alternative hosts to the first generation of artificial Moser's mites was 1:3. After 6-14 days of incubation, mature swollen bodies appeared on the alternative hosts of Moser's mites.

[0012] (3) Transfer of the inflated bodies of the Moser mite: In an incubator at 15-18℃, 19-22℃, or 23-26℃, remove the larvae of the red pine moth, fir moth, cotton bollworm, and Asian corn borer that have grown mature inflated bodies from the mite receiving box and transfer them to a mite storage tube. Then, use double-sided tape to stick a circle of double-sided tape on the outside of the mite storage tube and place it in an incubator at 25±1℃. Wait for the mature inflated bodies to breed offspring mites, and then count the time when the mites emerge.

[0013] Table 1 Comparison of the number of bulging bodies formed by alternative hosts under different temperature conditions

[0014] temperature 15-18℃ 19-22℃ 23-26℃ Alternative host species The maximum number of expanded bodies The maximum number of expanded bodies The maximum number of expanded bodies Red pine shoot borer 56 100 200 Abies abies 64 102 142 cotton bollworm 254 178 218 Asian corn borer 107 102 142

[0015] Result explanation: This result only replaces the maximum number of bloated bodies formed by the host under different temperature conditions, and can parasitize the host to produce bloated bodies under different temperature conditions.

Claims

1. The artificial breeding technology of Moser mites under different temperature conditions is characterized in that: The following steps are involved: (1) The first generation of artificially bred Moser mites: The Moser mites with wild characteristics were collected from the wild, placed in a culture dish at a temperature of 25±1°C and a relative humidity of 50±5%, and cultured for about 7 days. After the mites had young mites, healthy honey bee larvae were placed in a mite storage tube, and then placed in a culture dish with young mites. When all the young mites developed into mature mites, the mites in the storage tube were used as the first generation of artificial mites. (2) Preparation of alternative hosts for Moser's mites: The larvae of red pine moth, fir moth, cotton bollworm and Asian corn borer are used as alternative hosts. The alternative hosts and the first generation of artificially bred Moser's mites are placed in a mite inoculation box at 16-25°C and relative humidity of 45-60%. The ratio of the alternative hosts to the first generation of artificially bred Moser's mites is 1:

3. The boxes are placed in an incubator for 6-14 days to obtain mature, expanded abdominal bodies of the alternative hosts for Moser's mites. (3) Transfer of the inflated bodies of Moser's mites: The larvae of red pine moth, fir moth, cotton bollworm and Asian corn borer that have been inoculated with mature inflated bodies of Moser's mites are taken out of the mite inoculation box and placed into mite storage tubes respectively. A circle of double-sided tape is then attached to the outside of the tubes and the tubes are placed in an incubator to wait for the mature inflated bodies to breed offspring mites.

2. The artificial breeding technology of Moser mites under different temperature conditions according to claim 1, characterized in that: The preparation of the alternative host for Pleurotus erythroxylum mosserii and the setting of temperature and humidity, the present invention selected four kinds of Lepidoptera larvae, under the condition of the same relative humidity, set three different temperature gradients for breeding.

3. The artificial breeding technology of Moser mites under different temperature conditions according to claim 1, characterized in that: The mite receiving box is composed of a glass base with a diameter of 90 mm and a height of 2 cm, a glass cover with a diameter of 90 mm and a height of 1.5 cm, and a semicircular glass cover with a bottom diameter of 90 mm, an upper diameter of 14.6 cm, and a height of 4.8 cm. The glass base is divided into three parts by a glass plate with a height of 2 cm. The substitute host and the first generation of artificially bred Moser's Pleurotus eryngii are placed inside the base. A layer of vaseline is applied to the outer circle of the semicircular glass cover to prevent Moser's Pleurotus eryngii from escaping.

4. The artificial breeding technology of Moser mites under different temperature conditions according to claim 1, characterized in that: The mite storage tube is a glass device with a diameter of 13 mm, a height of 54 mm, and an internal support rod of 8 mm, which is convenient for the mites to fully contact with the host.

5. The artificial breeding technology of Moser mites under different temperature conditions according to claim 1, characterized in that: The width of the double-sided adhesive tape on the outside of the mite storage tube is 3cm, or the mite storage tube opening is plugged with absorbent cotton.

6. The artificial breeding technology of Moser mites under different temperature conditions according to claim 3, characterized in that: The mite storage tube containing the mature puffy bodies of Pleurotus erythroxylum moserii is placed in an incubator at a temperature of 25±1° C. and a relative humidity of 45-60% to culture the tube until the mature puffy bodies breed offspring mites.

7. The use of Moze Pleurotus eryngii in parasitic larvae of red pine borer, fir borer, cotton bollworm and Asian corn borer, characterized in that: They are all lepidoptera insects with thin body walls, making it easy for Moser mites to bite and host.