Application of trans-2-hexenal as weed germination inhibitor

Abstract

The invention relates to the technical field of agricultural agents, in particular to application of trans-2-hexenal as a weed germination inhibitor. It is found for the first time that trans-2-hexenal has an inhibition effect on wheat field gramineous weed germination under a certain concentration, and then application of trans-2-hexenal as a weed germination inhibitor is provided. The invention provides a better application mode of the trans-2-hexenal in the soil, and defines a wheat sowing safety interval after the trans-2-hexenal is applied. Therefore, the trans-2-hexenal can be used for reducing the grassy weed seed bank in the wheat field soil, the occurrence of wheat field weeds is controlled from the source, the green prevention and control of wheat field weeds are realized, and the use of chemical herbicides is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural chemicals, and in particular to the application of trans-2-hexenal as a weed germination inhibitor. Background Art

[0002] Wheat is one of the main food crops. Weeds are the main pests that threaten wheat yield and quality safety. Chemical herbicides are currently the main technical means for weed control in wheat fields. However, with the changes in planting systems, industrial structure adjustments and the use of herbicides, the weed community in wheat fields has evolved, and difficult-to-control weeds such as Aegilops tauschii, which are insensitive to herbicides, have gradually become dominant weeds in wheat fields. The unscientific use of herbicides over the years has also led to frequent resistance of wheat field weeds. For example, studies have reported that wheat field weeds such as Japanese Alopecurus, Alopecurus dasyphylla, and Lolium have developed varying degrees of resistance to herbicides with different mechanisms of action, increasing the difficulty of weed control in wheat fields. In addition, during the use of herbicides, some herbicide residues pollute water, air and soil, posing a potential threat to human health. Based on the above problems, it is urgent to develop new green products and technologies for the prevention and control of wheat field weeds.

[0003] Trans-2-hexenal, also known as leaf aldehyde, has a molecular formula of C6H 10 O, is an aliphatic unsaturated olefin aldehyde, a kind of green volatile, which can be quickly synthesized and released by most green plants after being attacked. Trans-2-hexenal is a light yellow to colorless oily liquid with a light fruity fragrance. It is mainly found in peach, cucumber, apple, camphor oil and citronella oil. In industry, trans-2-hexenal is mainly produced by chemical synthesis rather than separation from natural products. Trans-2-hexenal is evaluated by the World Health Organization Expert Committee on Food Additives as a food-grade flavoring substance additive that is safe for human consumption and can be used to modulate food flavor. Trans-2-hexenal has certain antibacterial activity and is widely used in fruit and vegetable preservation. In addition, trans-2-hexenal has good fumigation toxicity to leek late-eye fungus gnats and has strong nematicidal activity against pine wood nematodes, wheat root-knot nematodes and wheat cyst nematodes. So far, there has been no report on the application of trans-2-hexenal in pre-emergence weed germination inhibitors to reduce soil weeds.

[0004] In view of this, the present invention is proposed. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides the use of trans-2-hexenal as a weed germination inhibitor, which, as a new weed control method, can efficiently, non-toxicly and safely eliminate the weed seed bank in the soil of wheat fields from the source.

[0006] Specifically, the technical solution of the present invention is as follows: In a first aspect, the present invention provides an application of trans-2-hexenal as a weed germination inhibitor in wheat fields: trans-2-hexenal inhibits weed germination in wheat fields, and the concentration for inhibiting weed germination in wheat fields is above 15 μL / L.

[0007] Preferably, the wheat field weeds are grass weeds.

[0008] Preferably, the wheat field weeds include at least one of Aegilops tauschii, Aegilops cylindrica, Alopecurus japonicus, Alopecurus macrostachya and Lolium perenne.

[0009] Preferably, the wheat field weeds include Aegilops tauschii, and the concentration of trans-2-hexenal that inhibits the germination of Aegilops tauschii is 15 μL / L or more; preferably 38 μL / L or more; more preferably 38-76 μL / L.

[0010] Preferably, the wheat field weeds include at least one of Aegilops cylindrica, Japanese Alopecurus, Alopecurus macrostriatus and Lolium perenne, and the concentration of trans-2-hexenal that inhibits the germination of Aegilops cylindrica, Japanese Alopecurus, Alopecurus macrostriatus or Lolium perenne is 38 μL / L or more.

[0011] Preferably, trans-2-hexenal is applied to the field by soil injection or soil mixing.

[0012] Preferably, the soil injection depth is 2±1 cm.

[0013] Preferably, the concentration of the trans-2-hexenal solution injected into the soil is above 38 μL / L, preferably 38-76 μL / L.

[0014] Preferably, the amount of trans-2-hexenal solution injected into the soil is 42 uL / kg or more, preferably 42-84 uL / kg.

[0015] Preferably, the depth of the mixed soil is 10±1 cm.

[0016] Preferably, the concentration of the trans-2-hexenal solution in the soil mixture is above 38 μL / L, preferably 38-76 μL / L.

[0017] Preferably, the amount of trans-2-hexenal solution used in the soil mixing is 42uL / kg or more, preferably 42-84uL / kg.

[0018] Preferably, after applying trans-2-hexenal in the field, there is an interval of at least 5 days before sowing wheat.

[0019] Preferably, wheat is sown after an interval of 5-20 days.

[0020] In a second aspect, the present invention provides a method for inhibiting weeds in a wheat field, wherein trans-2-hexenal is used as a weed germination inhibitor in a wheat field, and wheat is sown at least 5 days after trans-2-hexenal is applied in the field.

[0021] Beneficial effects: The present invention first discovered that trans-2-hexenal has an inhibitory effect on the germination of gramineous weeds in wheat fields at a certain concentration, and further provided the application of trans-2-hexenal as a weed germination inhibitor. The present invention provides a preferred application mode of trans-2-hexenal in soil, and clarifies the safe interval for wheat sowing after the application of trans-2-hexenal. Therefore, trans-2-hexenal can be used to reduce the seed bank of gramineous weeds in wheat field soil, control the occurrence of wheat field weeds from the source, realize green prevention and control of wheat field weeds, and reduce the use of chemical herbicides. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the drawings required for use in the embodiments or the description of the prior art will be described below.

[0023] Figure 1 Shows the effect of different concentrations of trans-2-hexenal on the germination of Aegilops tauschii.

[0024] Figure 2 The results show that trans-2-hexenal has an inhibitory effect on the germination of grass weeds in wheat fields.

[0025] Figure 3 The results show the inhibitory effect of trans-2-hexenal on the germination of Aegilops tauschii under different application modes.

[0026] Figure 4 Shows the emergence of wheat when sown at different intervals after application of trans-2-hexenal. DETAILED DESCRIPTION

[0027] The present invention provides the use of trans-2-hexenal as a weed germination inhibitor in wheat fields. As a new weed control method, the injection of trans-2-hexenal into the soil can inhibit the germination of grass weeds in the wheat fields, reduce the weed seed bank in the wheat field soil from the source, and has the advantages of high efficiency, easy volatility, non-toxicity, no residue, etc. Moreover, the safe interval for sowing wheat after the injection of trans-2-hexenal into the soil is short, and wheat can be sown after 5 days, which has certain safety.

[0028] Firstly, the present invention provides a new use of trans-2-hexenal.

[0029] Specifically, the present invention provides a novel use of trans-2-hexenal as a weed germination inhibitor.

[0030] Specifically, trans-2-hexenal is used to inhibit the germination of grass weeds in wheat fields.

[0031] Specifically, trans-2-hexenal is used to inhibit the germination of wheat field grass weeds Aegilops tauschii, Aegilops cylindrica, Alopecurus japonicus, Alopecurus macrostachya and Lolium perenne.

[0032] Specifically, in order to achieve the effect of inhibiting weed germination, the concentration of trans-2-hexenal is at least 15 μL / L, preferably, the concentration of trans-2-hexenal is at least 38 μL / L.

[0033] Specifically, for Aegilops tauschii, in order to achieve a good germination inhibition effect, the concentration of trans-2-hexenal is at least 15 μL / L. At this concentration, trans-2-hexenal has a significant germination inhibition effect on Aegilops tauschii. Most preferably, the concentration of trans-2-hexenal is at least 38 μL / L. At this concentration, trans-2-hexenal completely inhibits the germination of Aegilops tauschii seeds.

[0034] Specifically, for Aegilops cylindrica, Japanese Alopecurus, Alopecurus maximus and Lolium perenne, the present invention verifies that the most preferred concentration of trans-2-hexenal is at least 38 μL / L. At this concentration, trans-2-hexenal completely inhibits the germination of Aegilops cylindrica, Japanese Alopecurus, Alopecurus maximus and Lolium perenne seeds.

[0035] The present invention further provides an application method of trans-2-hexenal in soil.

[0036] Specifically, the application mode of trans-2-hexenal in soil is soil injection and / or soil mixing. Preferably, the application mode of trans-2-hexenal is soil injection.

[0037] Specifically, the injection depth of trans-2-hexenal in the soil is 2 cm.

[0038] Specifically, to achieve the effect of inhibiting weed germination, taking Aegilops tauschii as an example, the soil injection concentration of trans-2-hexenal is at least 38 μL / L, and preferably the soil injection concentration of trans-2-hexenal is at least 76 μL / L.

[0039] Specifically, in order to achieve the effect of inhibiting weed germination, taking Aegilops tauschii as an example, the soil mixing concentration of trans-2-hexenal is at least 38 μL / L.

[0040] The present invention further studies and clarifies the safe interval period of wheat sowing after trans-2-hexenal soil injection.

[0041] Specifically, wheat sowing at least 5 days after soil injection of trans-2-hexenal had no significant effect on wheat emergence.

[0042] Specifically, wheat sowing 5-20 days after soil injection of trans-2-hexenal had no significant effect on wheat emergence.

[0043] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] The endpoints and any values ​​of the ranges disclosed in this specification are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0045] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "specific implementations", or "some specific implementations" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0046] In the examples provided in this specification, if no specific techniques or conditions are specified, the techniques or conditions described in the literature in the field or the product instructions are used. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased through regular channels (among which trans-2-hexenal (original concentrated) is purchased from the market).

[0047] Example 1 This example studies the effect of trans-2-hexenal on the germination of Aegilops tauschii.

[0048] Select the seeds of Aegilops tauschii with uniform size, rinse them repeatedly with sterile water for 3 times and set aside. A round plastic petri dish with a diameter of 9 cm was used as a container for the experiment. A double layer of filter paper was placed on the bottom of the petri dish. 5 mL of sterile water was added to moisten the filter paper. 20 Aegilops tauschii seeds were evenly placed on the double layer of filter paper. The bottom of another petri dish was taken and a 2 cm long positive filter paper was fixed in the center of the bottom of the dish with double-sided tape. Different volumes of trans-2-hexenal were dripped on the filter paper. Then the two bottoms of the dishes were buckled and sealed with sealing film. The concentrations of trans-2-hexenal were set to 0, 3.8, 7.6, 15, 38 and 60 μL / L. After the operation, all petri dishes were placed in an artificial climate incubator (Zhejiang Top Instrument Co., Ltd.), the temperature was set to 25 / 22℃ (light / dark), the photoperiod was L / D=14 h / 10 h, and the light intensity was 320 μmol / m 2 s. After 96 h of culture, the number of Aegilops tauschii sprouts was counted, and those with radicle length greater than 3 mm were considered to have sprouted.

[0049] The above experiment was repeated 4 times. Figure 1 .

[0050] Figure 1 The results showed that trans-2-hexenal at concentrations of 3.8 μL / L and 7.6 μL / L had no significant effect on the germination of A. tauschii seeds. Trans-2-hexenal at concentrations of 15, 38 and 60 μL / L significantly inhibited the germination of A. tauschii seeds, and trans-2-hexenal at concentrations of 38 μL / L and 60 μL / L completely inhibited the germination of A. tauschii seeds.

[0051] Example 2 This example studies the inhibitory effect of trans-2-hexenal on the germination of grass weeds in wheat fields.

[0052] Common grass weeds in wheat fields, such as Aegilops cylindrica, Lolium perenne, Japanese Alopecurus japonicus and Alopecurus sphaerocephala, were used as test targets. Seeds of uniform size were selected and rinsed repeatedly with sterile water for 3 times for later use. The concentration of trans-2-hexenal was set to 0 μL / L and 38 μL / L, and other operations were the same as in Example 1. After 96 h of culture, the number of germinations of Aegilops cylindrica, Lolium perenne, Japanese Alopecurus japonicus and Alopecurus sphaerocephala were counted respectively, and the germination was considered when the radicle length was greater than 3 mm.

[0053] The above experiment was repeated 4 times. Figure 2 .

[0054] Figure 2The results showed that trans-2-hexenal at a concentration of 38 μL / L could significantly inhibit the germination of seeds of Aegilops cylindrica, Lolium perenne, Alopecurus japonicus and Alopecurus macrostachya. The germination of seeds of Aegilops cylindrica, Lolium perenne and Alopecurus macrostachya was completely inhibited, and the germination rate of Alopecurus macrostachya was less than 5%.

[0055] Example 3 This example studies the effects of different application modes of trans-2-hexenal on the germination of Aegilops tauschii.

[0056] Select Aegilops tauschii seeds of uniform size for later use.

[0057] Field soil that had not been sprayed with any herbicides was used in this experiment, and the soil was sieved for later use.

[0058] Soil injection treatment: The sieved soil and organic fertilizer were mixed in a ratio of 3:1 (v / v) and filled into plastic pots with a diameter of 11 cm and a height of 10 cm. Thirty grains of Aegilops tauschii were sown in each pot at a sowing depth of 3 cm. Trans-2-hexenal was drawn up using a disposable sterile syringe (without the needle) and injected into the center of the pot at a depth of 2 cm. The injection volumes were 0, 25, and 50 μL, respectively, corresponding to trans-2-hexenal concentrations of 0, 38, and 76 μL / L. After the operation, all pots were placed in an artificial climate incubator with a temperature set to 25 / 22°C (light / dark), a photoperiod of L / D=14 h / 10 h, and a light intensity of 320 μmol / m 2 s. The number of seedlings of Aegilops tauschii was counted 7 days after treatment. The above experiment was repeated 4 times.

[0059] Soil mixing treatment: The sieved soil was first mixed with organic fertilizer in a ratio of 3:1 (v / v), and then trans-2-hexenal was added and mixed thoroughly. The mixed soil filled plastic pots with a diameter of 11 cm and a height of 10 cm. 30 grains of Aegilops tauschii were sown in each pot at a sowing depth of 3 cm. The concentrations of trans-2-hexenal were set to 0, 38, and 76 μL / L, corresponding to an application rate of 0, 25, and 50 μL per pot, respectively. After the operation, all pots were placed in an artificial climate incubator with a temperature set to 25 / 22°C (light / dark), a photoperiod of L / D=14 h / 10 h, and a light intensity of 320 μmol / m 2 s.

[0060] The number of emerged Aegilops tauschii seedlings was counted 7 days after treatment.

[0061] The above experiment was repeated 4 times. Figure 3 .

[0062] Figure 3The results showed that both soil injection and soil mixing of trans-2-hexenal significantly inhibited the germination of A. tauschii seeds. Under the soil injection method, the inhibitory effect of trans-2-hexenal at a concentration of 76 μL / L on the germination of A. tauschii was significantly higher than that at 38 μL / L. At this concentration, the emergence rate of A. tauschii was 11.7%. Under the soil mixing method, the inhibitory effect of trans-2-hexenal at a concentration of 76 μL / L on the germination of A. tauschii was not significantly different from that at 38 μL / L. The emergence rate of A. tauschii was higher than 40% at both treatment concentrations.

[0063] Example 4 This embodiment studies the safe interval period for wheat sowing.

[0064] The wheat variety is AK58.

[0065] Field soil that had not been sprayed with any herbicides was used in this experiment, and the soil was sieved for later use.

[0066] The sieved soil and organic fertilizer were thoroughly mixed at a ratio of 3:1 (v / v). The mixed soil filled a plastic pot with a diameter of 11 cm and a height of 10 cm. Trans-2-hexenal was injected into the center of the pot using a disposable sterile syringe. The injection depth was 2 cm, the injection volume was 50 μL, and the injection concentration was 76 μL / L. Wheat was sown 0, 5, 10, 15, and 20 days after trans-2-hexenal injection, with 30 seeds per pot and a sowing depth of 3 cm. The blank control treatment was injected with an equal volume of sterile water, and wheat was sown 0 days after injection, with 30 seeds per pot and a sowing depth of 3 cm. After the operation, all pots were placed in an artificial climate incubator with a temperature set to 25 / 22°C (light / dark), a photoperiod of L / D=14 h / 10 h, and a light intensity of 320 μmol / m 2 s. The number of seedlings of Aegilops tauschii was counted 7 days after the treatment with trans-2-hexenal.

[0067] The above experiment was repeated 4 times. Figure 4 .

[0068] Figure 4 The results showed that the emergence rate of wheat sown on day 0 after trans-2-hexenal injection was 1.67%, which was significantly lower than that of the control treatment. The emergence rates of wheat sown on days 5, 10, 15 and 20 after trans-2-hexenal injection were not significantly different from those of the control treatment.

[0069] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. The use of trans-2-hexenal as a weed germination inhibitor in wheat fields, characterized in that: Trans-2-hexenal inhibits the germination of weeds in wheat fields, and the concentration for inhibiting the germination of weeds in wheat fields is above 15 μL / L.

2. The application according to claim 1, characterized in that: The wheat field weeds are grass weeds; Preferably, the wheat field weeds include at least one of Aegilops tauschii, Aegilops cylindrica, Alopecurus japonicus, Alopecurus macrostachya and Lolium perenne.

3. The application according to claim 2, characterized in that: The wheat field weeds include Aegilops tauschii, and the concentration of trans-2-hexenal that inhibits the germination of Aegilops tauschii is 15 μL / L or more, preferably 38 μL / L or more, and more preferably 38-76 μL / L.

4. The application according to claim 2, characterized in that: The wheat field weeds include at least one of Aegilops cylindrica, Japanese Alopecurus, Alopecurus longituba and Lolium perenne, and the concentration of trans-2-hexenal that inhibits the germination of Aegilops cylindrica, Japanese Alopecurus, Alopecurus longituba or Lolium perenne is 38 μL / L or more.

5. The use according to any one of claims 1 to 4, characterized in that: Trans-2-hexenal is applied to the field by soil injection or soil mixing.

6. The use according to claim 5, characterized in that: The depth of soil injection is 2±1 cm; and / or, the concentration of the trans-2-hexenal solution injected into the soil is 38 μL / L or more, preferably 38-76 μL / L; And / or, the amount of trans-2-hexenal solution injected into the soil is 42 μL / kg or more, preferably 42-84 μL / kg.

7. The use according to claim 5, characterized in that: The depth of the soil mixing is 10±1cm; and / or, the concentration of the trans-2-hexenal solution in the soil mixture is 38 μL / L or more, preferably 38-76 μL / L; And / or, the amount of trans-2-hexenal solution used in the soil mixing is 42 μL / kg or more, preferably 42-84 μL / kg.

8. The use according to any one of claims 1 to 7, characterized in that: After applying trans-2-hexenal in the field, wait at least 5 days before sowing wheat; Preferably, wheat is sown after an interval of 5-20 days.

9. A method for suppressing weeds in wheat fields, characterized in that: Trans-2-hexenal is used as a weed germination inhibitor in wheat fields. After trans-2-hexenal is applied in the field, wheat should be sown at least 5 days later.

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