Attenuating and desensitizing feeding material for apitherapy apis cerana, preparation method of feeding material and attenuating and desensitizing feeding method for apitherapy apis cerana

Abstract

The invention provides a feeding material for reducing toxicity and desensitization of apitherapy Chinese bees, a preparation method of the feeding material and a feeding method for reducing toxicity and desensitization of the apitherapy Chinese bees. The toxicity-reducing and sensitivity-reducing feeding material for apitherapy apis cerana comprises the following components: 2-4 parts of honeysuckle flowers; 2-4 parts of wild chrysanthemum flower; 4-6 parts of colocasia esculenta petiole sections; 1-3 parts of perilla leaf; 2-4 parts of flos puerariae; 1-3 parts of licorice root; and 2-3 parts of honey. According to the feeding material, the honeysuckle flower, the wild chrysanthemum flower, the taro petiole section, the perilla leaf, the flower of kudzuvine, the liquorice root and the honey are compounded for use, so that the content of melittin, phospholipase and hyaluronidase of the apis cerana can be effectively reduced, the pain feeling of a patient during apis cerana is reduced, and the allergy degree of the patient after apis cerana is also reduced; the survival rate of apitherapy apis cerana in the off-box process and the survival rate of the apis cerana in the feeding process can be improved.

Description

Technical Field

[0001] The present disclosure relates to the technical field of bee therapy, and in particular to a feed for reducing the toxicity and allergy of bees in bee therapy and a preparation method thereof, as well as a feeding method for reducing the toxicity and allergy of bees in bee therapy. Background Art

[0002] Apitherapy is a traditional Chinese medicine therapy. Scientific research shows that bee venom has good effects on rheumatism, asthma, low immunity, etc. Therefore, there are many large-scale bee therapy hospitals or bee therapy clinics across the country. At present, bee therapy in the medical field mostly uses live Chinese bees for direct stinging. However, the bee venom of Chinese bees directly raised in nature is highly toxic, which causes obvious local pain in patients after bee therapy, and is prone to allergic reactions such as redness, swelling, itching, etc. It is not suitable for children, weak constitutions, and adults who are afraid of pain.

[0003] In order to solve the above-mentioned technical problems, Chinese patent document No. CN101317631B discloses a bee attenuated feed for bee therapy and a preparation method thereof. By preparing the attenuated feed, the feed can be used to attenuate the toxicity of bees, so that patients will not feel obvious pain when receiving bee therapy. The feed is better suitable for children, adults with weak constitutions and those who are afraid of pain, thereby expanding the applicable population of bee therapy.

[0004] Although the above-mentioned bee attenuated feed is compounded with chrysanthemum, perilla leaf, kudzu flower, licorice and honey to reduce the amount of bee venom secretion and the secretion cycle of Chinese bee venom, and effectively reduce the content of bee venom peptide and bee venom peptide on the bee sting; it is unable to effectively reduce the content of allergic components such as hyaluronidase and phospholipase A2, resulting in patients with weak constitutions still having serious allergies after bee therapy. In addition, since the above-mentioned bee attenuated feed has a poor detoxification effect, for the Lingnan open-air breeding base in the humid and hot Lingnan, the survival time of the bees after being transferred to the bee therapy box is low due to the influence of the humid and hot environment, resulting in a high mortality rate of bees during bee therapy and a high cost of bee therapy. Summary of the invention

[0005] The purpose of the present invention is to overcome the shortcomings of the prior art and to provide a feed for reducing toxicity and hyposensitivity in apitherapy bees, which reduces the content of bee venom peptide, phospholipase and hyaluronidase in apitherapy bees; and improves the rearing process and survival rate of apitherapy bees out of the hive, and a preparation method thereof, as well as a rearing method for reducing toxicity and hyposensitivity in apitherapy bees.

[0006] The purpose of this disclosure is achieved through the following technical solutions:

[0007] A feed for reducing toxicity and allergy of bees in apitherapy, comprising the following components by weight:

[0008]

[0009] A method for preparing a feed for reducing toxicity and allergy of bees in apitherapy, comprising the following steps:

[0010] blanching the fresh taro stem segments to obtain processed taro stem segments for later use;

[0011] Add honeysuckle, wild chrysanthemum, kudzu flower and liquorice into water for decoction to obtain Chinese medicine mixed water A;

[0012] Adding perilla leaves and the processed taro stem segments to the Chinese medicine mixed water A for secondary decoction to obtain Chinese medicine mixed water B;

[0013] Filtering the Chinese medicine mixed water B to obtain Chinese medicine water;

[0014] The Chinese medicinal water is mixed with honey to obtain the bee-therapy feed for reducing toxicity and allergy in any of the above embodiments.

[0015] In one embodiment, the blanching operation comprises the following specific steps:

[0016] Use high heat to simmer until the water boils for 2min to 5min.

[0017] In one embodiment, the one-time decoction operation includes the following specific steps:

[0018] First use high heat to boil until the water boils, then switch to low heat and simmer for 20 to 40 minutes.

[0019] In one embodiment, the secondary decoction operation includes the following specific steps:

[0020] First use high heat to boil until the water boils, then switch to medium heat and simmer for 5 to 20 minutes.

[0021] In one embodiment, the amount of water used is 500 ml of water per 200 grams of the main ingredient of the raw material.

[0022] In one embodiment, the weight ratio of the Chinese medicine water and the honey is 1: (1-1.5).

[0023] A method for raising bees for reducing toxicity and allergy in apitherapy, comprising the following steps:

[0024] The feed for reducing toxicity and hyposensitivity of bees in apitherapy as described in any of the above embodiments is prepared by using the method for preparing the feed for reducing toxicity and hyposensitivity of bees in apitherapy as described in any of the above embodiments;

[0025] The bee therapy Chinese bee attenuated and hypoallergenic feed is used to feed the bee for 3 to 12 days to obtain the bee therapy Chinese bee;

[0026] The feeding amount is 100 g to 300 g per feeding; the feeding time is 16:00 to 18:00 in the evening; and the feeding time interval is (24h to 72h) per feeding.

[0027] In one embodiment, the step of feeding the Chinese bees with the apitherapy Chinese bee attenuated and hypoallergenic feed for 3 to 12 days also includes the following specific steps:

[0028] The feed for reducing the toxicity and sensitivity of bees in apitherapy is transported to a feeding trough in a beehive through a feeding device, and the temperature in the beehive is controlled to be 25° C. to 38° C.

[0029] In one embodiment, in the step of feeding the Chinese bees with the feed for reducing toxicity and allergy of the apitherapy Chinese bees for 3 days to 12 days, the apitherapy Chinese bee feeding method for reducing toxicity and allergy of the apitherapy Chinese bees further includes the following steps:

[0030] The feeding Chinese bees are subjected to lighting operation in the box, and the lighting operation time is 1min to 10min.

[0031] Compared with the prior art, the present invention has at least the following advantages:

[0032] 1) Honeysuckle contains chlorogenic acid, caffeic acid and other organic acid compounds, which have the effect of clearing away heat and detoxifying; wild chrysanthemum contains flavonoids, which also have the effect of clearing away heat and detoxifying; taro stem segments contain natural saponins, vitamin C and isoflavones, which have the effect of clearing heat, anti-inflammatory and analgesic; especially when used in combination with flavonoids and phenolic acid compounds contained in perilla leaves, isoflavones contained in kudzu flowers, flavonoids and triterpenoids contained in licorice and honey, it can effectively reduce the content of bee venom peptide, phospholipase and hyaluronidase in bees in bee therapy, which not only reduces the pain of patients during bee therapy, but also reduces the degree of allergy of patients after bee therapy, and is more suitable for patients with weak constitution.

[0033] 2) By compounding taro stem segments, honeysuckle and wild chrysanthemum, the addition of cold taro stem segments, honeysuckle and wild chrysanthemum can play a good synergistic effect, so as to ensure that the prepared bee therapy Chinese bee attenuation and desensitization feed has a good effect of clearing away heat and detoxification, effectively solve the problem of damp heat detoxification of Chinese bees in the humid and hot Lingnan open-air breeding base, thereby providing an effective guarantee for the healthy growth of Chinese bees; and, because the addition of taro stem segments can better neutralize the organic acid compounds in the bee therapy Chinese bee attenuation and desensitization feed, it is ensured that the digestive system of the Chinese bees after consumption will not be disturbed, and the problem of low survival rate of Chinese bees caused by eating foods containing more natural saponins or eating foods with a high content of organic acid compounds that affect the digestive system of Chinese bees is effectively avoided, further ensuring the healthy growth of Chinese bees, which is beneficial to improving the breeding process of Chinese bees and the survival rate of Chinese bees out of the hive, thereby reducing the mortality rate of Chinese bees, and further reducing the cost of bee therapy. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present disclosure and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0035] Figure 1 This is a flow chart of a method for preparing a feed for reducing toxicity and allergy in bee therapy according to an embodiment of the present invention;

[0036] Figure 2 It is a flow chart of a method for raising bees for reducing toxicity and allergy in apitherapy according to an embodiment of the present invention;

[0037] Figure 3 This is a real picture of the feeding of bees for reducing toxicity and allergy in apitherapy according to an embodiment of the present invention;

[0038] Figure 4 This is a physical diagram comparing the 3d and 7d out-of-box survival rates of bees in the apitherapy of Example 1 of the present invention. DETAILED DESCRIPTION

[0039] In order to facilitate the understanding of the present disclosure, the present disclosure will be described more fully below with reference to the relevant drawings. The preferred embodiments of the present disclosure are given in the drawings. However, the present disclosure can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present disclosure more thoroughly and comprehensively understood.

[0040] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0041] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present disclosure. The terms used herein in the specification of the present disclosure are only for the purpose of describing specific embodiments and are not intended to limit the present disclosure. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0042] The present invention discloses a feed for reducing toxicity and allergy of bees in bee therapy, which comprises the following components by weight: 2 to 4 parts of honeysuckle; 2 to 4 parts of wild chrysanthemum; 4 to 6 parts of taro stem segments; 1 to 3 parts of perilla leaves; 2 to 4 parts of kudzu flowers; 1 to 3 parts of liquorice; and 2 to 3 parts of honey.

[0043] The above-mentioned bee therapy Chinese bee detoxification and hypoallergenic feeding feed, by compounding honeysuckle, wild chrysanthemum, taro stem segments, perilla leaves, kudzu flowers, licorice and honey, can not only effectively reduce the content of bee venom peptide, phospholipase and hyaluronidase in bee therapy Chinese bees, thereby reducing the pain of patients during bee therapy, but also reduces the degree of allergy of patients after bee therapy; it also helps to improve the survival rate of bee therapy Chinese bees out of the hive and the survival rate of the Chinese bees during the breeding process.

[0044] In order to better understand the technical scheme and beneficial effects of the present disclosure, the present disclosure is further described in detail below in conjunction with specific embodiments. In one embodiment, the feed for reducing toxicity and allergy of bees in bee therapy includes the following components by weight: 2 to 4 parts of honeysuckle; 2 to 4 parts of wild chrysanthemum; 4 to 6 parts of taro stem segments; 1 to 3 parts of perilla leaves; 2 to 4 parts of kudzu flowers; 1 to 3 parts of licorice; and 2 to 3 parts of honey.

[0045] It can be understood that since honeysuckle contains chlorogenic acid, caffeic acid and other organic acid compounds, it has the effect of clearing away heat and detoxifying; wild chrysanthemum contains flavonoids, which also have the effect of clearing heat and detoxifying; taro stem segments contain natural saponins, vitamin C and isoflavones, which have the effects of clearing heat, anti-inflammatory and analgesic; especially when combined with flavonoids and phenolic acid compounds contained in perilla leaves, isoflavones contained in kudzu flowers, flavonoids and triterpenoids contained in licorice and honey, it can effectively reduce the content of bee venom peptides, phospholipase and hyaluronidase in bees in bee therapy, which not only reduces the pain of patients during bee therapy, but also reduces the degree of allergy of patients after bee therapy, and is more suitable for patients with weaker constitution.

[0046] It can be understood that by compounding taro stem segments, honeysuckle and wild chrysanthemum, the addition of taro stem segments, honeysuckle and wild chrysanthemum with cold properties can play a better synergistic and synergistic role, so as to ensure that the prepared bee therapy Chinese bee detoxification and hypoallergenic feed has a good effect of clearing away heat and detoxifying, effectively solving the problem of damp heat detoxification of Chinese bees in the relatively humid and hot Lingnan open-air breeding base, thereby providing an effective guarantee for the healthy growth of Chinese bees; and, because the addition of taro stem segments can better neutralize the organic acid compounds in the bee therapy Chinese bee detoxification and hypoallergenic feed, it is ensured that the digestive system of the Chinese bees after consumption will not be disturbed, and effectively avoid the problem of low survival rate of Chinese bees due to eating foods containing more natural saponins or eating foods with a high content of organic acid compounds, which affects the digestive system of Chinese bees, further ensuring the healthy growth of Chinese bees, and is beneficial to improving the breeding process and survival rate of Chinese bees out of the hive, thereby reducing the mortality rate of Chinese bees, and then reducing the cost of bee therapy.

[0047] It should be noted that although taro stems are a commonly used folk medicine for detoxifying bee stings, people simply apply the juice of taro stems to the site of the bee sting to detoxify the sting; if fresh taro stems are directly used with honeysuckle, wild chrysanthemum, kudzu flower and licorice for decoction, the final bee therapy feed for reducing toxicity and hyposensitization of Chinese bees will contain more natural saponins, and more natural saponins can easily irritate the gastrointestinal mucosa of Chinese bees, causing digestive symptoms such as diarrhea during the breeding process, resulting in a low survival rate of Chinese bees.

[0048] Therefore, the present disclosure also provides a method for preparing a feed for reducing toxicity and allergy of bees in bee therapy. First, fresh taro stem segments are blanched to remove most of the natural saponins in the taro stem segments to obtain treated taro stem segments for standby use; then, honeysuckle, wild chrysanthemum, kudzu flower and licorice are added to water for a decoction operation so that the detoxifying active substances in the honeysuckle, wild chrysanthemum, kudzu flower and licorice can be more completely dissolved in the water to obtain Chinese medicine mixed water A; then, perilla leaves and the treated taro stem segments are added to the Chinese medicine mixed water A for a second decoction operation so that the detoxifying active substances in the perilla leaves and the taro stem segments can be more completely dissolved in the water to obtain Chinese medicine mixed water B; the Chinese medicine mixed water B is filtered to remove the filter residue to obtain Chinese medicine water; the Chinese medicine water is mixed with honey to obtain the feed for reducing toxicity and allergy of bees in bee therapy in any of the above embodiments.

[0049] The method for preparing the above-mentioned bee therapy feed for reducing toxicity and allergy of Chinese bees, by blanching fresh taro stem segments, effectively removes most of the natural saponins in the taro stem segments, while a small amount of natural saponins, a certain amount of vitamin C and a relatively high level of isoflavone compounds can be retained in the taro stem segments, so as to ensure that a small amount of natural saponins dissolved in the taro stem segments in the subsequent secondary decoction operation can neutralize a small amount of organic acid compounds in the Chinese medicine mixed water A, effectively preventing the Chinese bees from irritating their intestinal mucosa due to eating foods containing more natural saponins or foods with a high content of organic acid compounds, thereby causing the Chinese bees to easily have diarrhea during the breeding process, etc. digestive symptoms leading to the low survival rate of Chinese bees; and putting the perilla leaves and taro stem segments last can effectively reduce the loss of phenolic acid compounds in the perilla leaves, vitamin C and flavonoid compounds in the taro stem segments, so as to ensure that the prepared bee therapy Chinese bee attenuated and desensitized feed can effectively reduce the content of bee venom peptide, phospholipase and hyaluronidase in bee therapy Chinese bees, and reduce the probability of diarrhea in the bees during the breeding process, thereby improving the survival rate of the bees during the breeding process, and then providing healthy bees for bee therapy, to ensure that healthy bees can reliably and stably release bee venom peptide in the body, thereby ensuring the stability of the bee therapy effect.

[0050] See also Figure 1 As shown, the method for preparing the feed for reducing toxicity and allergy of bees in apitherapy in one embodiment includes some or all of the following steps:

[0051] S110, blanching the fresh taro stem segments to remove most of the natural saponin in the taro stem segments, and obtaining processed taro stem segments for later use.

[0052] It should be noted that since fresh taro stem segments contain more natural saponins, if they are not processed, the feed prepared for bee therapy to reduce toxicity and sensitivity of Chinese bees will contain more natural saponins. After the Chinese bees eat it, the more natural saponins will sting the gastrointestinal mucosa of the Chinese bees, causing digestive symptoms such as diarrhea during the breeding process, resulting in a low survival rate of the Chinese bees.

[0053] Therefore, in the present disclosure, the fresh taro stem segments are blanched to remove most of the natural saponins in the taro stem segments, while a small amount of natural saponins, a certain amount of vitamin C and a relatively high amount of isoflavone compounds can be retained in the taro stem segments, so as to ensure that the small amount of natural saponins dissolved in the taro stem segments in the subsequent secondary decoction operation can neutralize the small amount of organic acid compounds in the Chinese medicine mixed water A, and effectively avoid the problem that the intestinal mucosa of the Chinese bees is stimulated by eating foods containing more natural saponins or more organic acid compounds, so as to cause the Chinese bees to be prone to diarrhea and other digestive symptoms during the breeding process, resulting in a low survival rate of the Chinese bees, thereby improving the survival rate of the Chinese bees during the breeding process and the survival rate of the Chinese bees out of the hive, and thus providing healthy and vigorous Chinese bees for bee therapy, ensuring that healthy Chinese bees can reliably and stably release bee venom peptides in the body, thereby ensuring the stability of the bee therapy effect.

[0054] It is worth mentioning that since fresh taro stem segments and dried taro stem segments contain more active substances that clear heat, fight inflammation and relieve pain, such as vitamin C and isoflavone compounds, the prepared bee therapy feed for reducing toxicity and hyposensitivity of Chinese bees contains more vitamin C and isoflavone compounds, ensuring that the prepared bee therapy feed for reducing toxicity and hyposensitivity of Chinese bees has a good effect of clearing heat and detoxifying, effectively solving the problem of heat and damp detoxification of Chinese bees in the humid and hot Lingnan open-air breeding base, thereby providing an effective guarantee for the healthy growth of Chinese bees.

[0055] In one of the embodiments, the blanching operation includes the following specific steps: decocting with high heat until the water boils for 2 minutes to 5 minutes, so as to better remove more natural saponins in the taro stem segments, while retaining a higher content of vitamin C, higher isoflavone compounds and a small amount of natural saponins, so as to ensure that the subsequent preparation of the bee therapy feed for reducing toxicity and allergy for Chinese bees can meet the requirements of reducing toxicity and allergy, and also ensure that the digestive system of the Chinese bees after consumption will not be disturbed, thereby ensuring the healthy growth of the Chinese bees, that is, ensuring that the vitality of the Chinese bees is strong, thereby improving the survival rate of the Chinese bees after leaving the hive and the survival rate of the Chinese bees during the breeding process.

[0056] It is understandable that since the taro stem section closer to the taro has a higher content of natural saponins, a simple blanching operation cannot effectively remove the natural saponins content of this section of the taro stem, resulting in a higher content of natural saponins in the subsequent bee therapy feed for reducing toxicity and allergy to bees, which affects the digestive system of the bees and causes a low survival rate of the bees. Therefore, in one of the embodiments, first, obtain fresh, complete and mature taro stem strips; wash the taro stem strips to remove dust on the surface; then remove (1 / 10 to 1 / 3) of the taro stem segments that are closer to the taro to remove the taro stem segments with a higher content of natural saponins, and then cut the remaining (2 / 3 to 9 / 10) of the taro stem strips into segments to obtain taro stem segments, so as to ensure that the final prepared bee therapy feed for reducing toxicity and allergy to Chinese bees has a lower content of natural saponins. In this way, while ensuring that the subsequently prepared bee therapy feed for reducing toxicity and allergy to Chinese bees meets the requirements of reducing toxicity and allergy, it also ensures that the digestive system of the Chinese bees after consumption will not be disturbed, thereby ensuring the healthy growth of the Chinese bees, that is, ensuring that the vitality of the Chinese bees is strong, thereby improving the survival rate of the Chinese bees after leaving the hive and the survival rate of the Chinese bees during the breeding process.

[0057] In one embodiment, the taro stem segment closer to the taro can be removed in an amount of 1 / 10, 1 / 9, 1 / 8, ..., 1 / 4, or 1 / 3, and the operator can select and use it according to actual needs. Further, in a preferred embodiment, the taro stem segment closer to the taro is removed in an amount of 1 / 3.

[0058] It can be understood that since the content of isoflavone compounds and vitamin C in fresh and mature taro stems is higher than that in immature taro stems, the use of fresh and mature taro stems is beneficial to the preparation of apitherapy feed for reducing toxicity and hyposensitivity for Chinese bees, which has better detoxification and heat-clearing and detoxification effects and less interference with the digestive system of Chinese bees, so that it is better suitable for the breeding of Chinese bees in the humid and hot Lingnan open-air breeding base.

[0059] It can be understood that in order to ensure that the natural saponins in the taro stem strips can be quickly removed during the blanching operation, in one embodiment, before the step of cutting the remaining (2 / 3 to 9 / 10) taro stem strips into segments and after the step of removing the (1 / 10 to 1 / 3) taro stem segments closer to the taro, the following step is also included: the remaining (2 / 3 to 9 / 10) taro stem strips are pierced so that the remaining (2 / 3 to 9 / 10) taro stem strips can be penetrated on the longitudinal horizontal plane to form multiple through-holes, so that the natural saponins in the taro stem strips can quickly flow out from the multiple through-holes to achieve the removal operation of most of the natural saponins in the taro stem segments.

[0060] In one of the embodiments, a piercing tool is used to perform all-round piercing operations on the remaining (2 / 3 to 9 / 10) taro stems, so that a plurality of through-punctures are distributed in a matrix in the length direction of the taro stems, and the spacing between each through-puncture is controlled to be 0.1 cm to 2 cm, so as to ensure that the plurality of through-punctures can be evenly distributed on the taro stems, thereby ensuring that the distribution of the plurality of through-punctures on the taro stems is more appropriate, so as to ensure that the taro stem segments can achieve a rapid removal of the natural saponin inside during the subsequent shorter blanching operation time.

[0061] In one embodiment, the spacing between each through-hole is 0.1 cm, 0.2 cm, 0.3 cm, 0.4 cm, 0.5 cm, 0.6 cm, 0.7 cm, 0.8 cm, 0.9 cm, ..., 1.0 cm, 1.1 cm, 1.5 cm, 1.8 cm, 2.0 cm, and the operator can select according to actual needs. Further, in a preferred embodiment, the spacing between each through-hole is 0.2 cm.

[0062] It can be understood that although the additional through-holes are conducive to the rapid outflow of natural saponins inside, if the outflowing natural saponins are not processed in time, the outflowing natural saponins will easily oxidize and adhere to the peripheral edge of the outer wall of the through-holes, causing the inner wall of the through-holes to form a brown-black colloid. These colloids will adhere to the inner wall of the through-holes to form a blocking barrier, which is not conducive to the rapid release of natural saponins and isoflavone compounds in the taro stem segment. Therefore, in one embodiment, after the remaining (2 / 3 to 9 / 10) taro stems are pierced, the remaining (2 / 3 to 9 / 10) taro stems that have completed the piercing operation are immediately soaked in clean water. In this way, on the one hand, the clean water can better wash away the natural saponin flowing out of the through-holes, reducing the problem of brown-black colloid adhering to the inner wall of the through-holes. On the other hand, the clean water can play an isolating role, effectively avoiding the problem of easy oxidation of vitamin C and isoflavone compounds, thereby ensuring that the final prepared bee therapy bee detoxification and hyposensitization feed has a high content of isoflavone compounds and vitamin C.

[0063] In one of the embodiments, when the remaining (2 / 3 to 9 / 10) taro stem strips that have completed the hole-piercing operation are immediately immersed in clean water, the remaining (2 / 3 to 9 / 10) taro stem strips need to be completely immersed in the clean water to prevent the taro stem strips from coming into contact with the outside air and causing the inner wall of the hole to adhere to brown-black colloid.

[0064] In one embodiment, in the step of cutting the remaining (2 / 3 to 9 / 10) taro stem strips, the cutting line of the cutting section intersects with a plurality of longitudinally straight through holes respectively, ensuring that the cutting end face of the taro stem section after the cutting section is formed with a plurality of inwardly recessed semicircular flow holes, thereby increasing the surface area of ​​the taro stem section, which is conducive to the faster outflow and release of the natural saponin inside the taro stem section, so as to achieve the rapid removal of the natural saponin in the taro stem strips. In addition, a plurality of longitudinally straight through holes can provide cutting positioning points for cutting sections, so that the operator can cut sections quickly and accurately, to ensure that the length of the taro stem section after batch cutting remains basically consistent, which is conducive to subsequent batch processing.

[0065] In one of the embodiments, the length of the taro stem segment is 1 cm to 5 cm to ensure that the length of the taro stem segment is more appropriate, so that most of the natural saponins in the taro stem segment can be released during the subsequent blanching operation, while a small amount of natural saponins, a certain amount of vitamin C and a relatively high amount of isoflavone compounds can be retained in the taro stem segment. In this way, while ensuring that the subsequently prepared bee therapy feed for reducing toxicity and allergy to Chinese bees meets the requirements of reducing toxicity and allergy, it also ensures that the digestive system of the Chinese bees after consumption will not be disturbed, that is, a small amount of natural saponins released in the taro stem segment can neutralize a small amount of organic acid compounds in the Chinese medicine mixed water A, effectively avoiding the problem of low survival rate of Chinese bees due to eating foods containing more natural saponins or eating foods with a high content of organic acid compounds, which affects the digestive system of Chinese bees, thereby ensuring the healthy growth of Chinese bees and further improving the survival rate of Chinese bees after leaving the hive.

[0066] In one embodiment, the length of the taro stem segment is 3 cm.

[0067] In one embodiment, after the remaining (2 / 3 to 9 / 10) taro stem strips are cut into segments, the cut taro stem segments need to be immediately immersed in clean water for blanching, so as to further reduce the problem of brown-black colloid adhering to the cut end surfaces of the taro stem segments after cutting.

[0068] In one embodiment, the step of blanching the fresh taro stem segments also includes the following specific steps: the fresh taro stem segments are covered with clean water by 1 cm to 2 cm, and then high heat is turned on. When the water boils for 2 minutes to 5 minutes and the fresh taro stem segments just change color, the taro stem segments are picked up and set aside.

[0069] In one embodiment, clean water covers the fresh taro stem segment by 2 cm, then high heat is turned on and the water is boiled for 2 minutes to ensure that the clean water can still cover the taro stem segment when the water boils. In this way, during the blanching operation, a smaller amount of clean water and less heat energy can be used to better release natural saponins from the taro stem segment, so as to ensure that a small amount of natural saponins, a certain amount of vitamin C and a higher level of isoflavone compounds can be retained in the taro stem segment.

[0070] S120. Add honeysuckle, wild chrysanthemum, kudzu flower and liquorice into water and perform a decoction operation to obtain Chinese medicine mixed water A; to ensure that the organic acid compounds in honeysuckle, the flavonoid compounds in wild chrysanthemum, the isoflavone compounds in kudzu flower and the flavonoid compounds and triterpenoid compounds in liquorice can be well dissolved in water to obtain Chinese medicine mixed water A, which is helpful to prepare a feed for bee therapy that has better detoxification and allergy-reducing effect, good heat-clearing and detoxifying effect, and less interference with the digestive system of Chinese bees.

[0071] In one embodiment, the decoction operation includes the following specific steps: first use high heat to boil the water, and then use low heat to boil for 20 minutes to 40 minutes to ensure that the detoxifying active substances in honeysuckle, wild chrysanthemum, kudzu flower and licorice can be dissolved in the water more comprehensively, so as to prepare a bee therapy feed for reducing toxicity and allergy, which has good heat-clearing and detoxifying effects and less interference with the digestive system of the Chinese bee.

[0072] In one embodiment, the amount of water used is 500 ml of water for every 200 grams of the main raw material, to ensure that the added 500 ml of water can better dissolve the detoxifying active substances in honeysuckle, wild chrysanthemum, taro stem segments, kudzu flower and licorice, which is conducive to preparing a Chinese medicine water with better detoxification and hyposensitization effects, good heat-clearing and detoxifying effects, and less interference with the digestive system of the Chinese bee.

[0073] S130, adding perilla leaves and the processed taro stem segments to the Chinese medicine mixed water A for secondary decoction to obtain Chinese medicine mixed water B.

[0074] It can be understood that since the loss rate of phenolic acid compounds, flavonoid compounds in perilla leaves and vitamin C in taro stem segments is relatively high during the long-term decoction operation, adding the perilla leaves and the processed taro stem segments last can effectively reduce the serious loss of phenolic acid compounds, vitamin C and flavonoid compounds due to the long decoction operation time.

[0075] It can also be understood that since a small amount of natural saponins retained in the taro stem segments can be released during the secondary decoction operation, the released natural saponins can neutralize the small amount of organic acid compounds in the Chinese medicine mixed water A, effectively preventing the Chinese bees from irritating their intestinal mucosa due to eating foods containing more natural saponins or foods with higher content of organic acid compounds, causing the Chinese bees to easily suffer from diarrhea and other digestive symptoms during the breeding process, resulting in a low survival rate of the Chinese bees.

[0076] In one embodiment, the secondary decoction operation includes the following specific steps: first use high heat to boil the water, and then use medium heat to boil for 5 minutes to 20 minutes. While meeting the high dissolution rate of vitamin C in the taro stem segment, phenolic acid compounds and flavonoid compounds in the perilla leaves, the loss rate of vitamin C, flavonoid compounds and phenolic acid compounds is minimized.

[0077] S140, filtering the Chinese medicine mixed water B to effectively remove the filter residue of the Chinese medicine mixed water B, and obtaining Chinese medicine water with good detoxification and allergy-reducing effects, good heat-clearing and detoxifying effects, and less interference with the digestive system of Chinese bees.

[0078] S150, mixing the Chinese medicinal water with honey to obtain the bee-therapy feed for reducing toxicity and allergy in any of the above embodiments.

[0079] It can be understood that if the weight ratio of the Chinese medicinal water to the honey is lower than 1:1, the content of the Chinese medicinal water added will be too small and the effect of effectively reducing toxicity and allergy to Chinese bees within a shorter period of 7d to 12d will not be achieved; if the weight ratio of the Chinese medicinal water to the honey is higher than 1:1.5, the content of the Chinese medicinal water added will be too large, resulting in an excessive detoxification effect on Chinese bees, and the content of the bee venom peptide of the bee therapy Chinese bees finally prepared will be too small and a good therapeutic effect cannot be achieved. Therefore, in one embodiment, the weight ratio of the Chinese medicinal water to the honey is 1: (1 to 1.5) to ensure that the weight of the Chinese medicinal water and the honey is more appropriate, so as to ensure that the content of the bee venom peptide of the bee therapy Chinese bees finally prepared can achieve a good therapeutic effect, and also ensure that the effect of effectively reducing toxicity and allergy to Chinese bees is achieved in a shorter feeding time.

[0080] It can also be understood that when the Chinese medicine water and the honey are mixed in a weight ratio of 1: (1 to 1.5), it can ensure that the prepared bee therapy feed for reducing toxicity and allergy of Chinese bees is rich in major nutrients, and can provide good nutritional components for Chinese bees to better ensure the healthy growth of Chinese bees, thereby effectively reducing the survival rate of Chinese bees after leaving the hive and the survival rate during the Chinese bee breeding process.

[0081] It can also be understood that when the Chinese medicine solution and the honey are mixed in a weight ratio of 1: (1 to 1.5), it is ensured that the finally prepared bee therapy Chinese bee detoxification and hypoallergenic feed has good fluidity, so that the subsequent use of the feeding device can directly transport the bee therapy Chinese bee detoxification and hypoallergenic feed to the feeding trough in the beehive, without the need for manual opening of the box for feeding, which is not only simple to operate but also reduces the risk of stinging, thereby improving the safety of the feeding process.

[0082] The method for preparing the above-mentioned bee therapy feed for reducing toxicity and allergy of Chinese bees, by blanching fresh taro stem segments, effectively removes most of the natural saponins in the taro stem segments, while a small amount of natural saponins, a certain amount of vitamin C and a relatively high level of isoflavone compounds can be retained in the taro stem segments, so as to ensure that a small amount of natural saponins dissolved in the taro stem segments in the subsequent secondary decoction operation can neutralize a small amount of organic acid compounds in the Chinese medicine mixed water A, effectively preventing the Chinese bees from irritating their intestinal mucosa due to eating foods containing more natural saponins or foods with a high content of organic acid compounds, thereby causing the Chinese bees to easily have diarrhea during the breeding process, etc. digestive symptoms leading to the low survival rate of Chinese bees; and putting the perilla leaves and taro stem segments last can effectively reduce the loss of phenolic acid compounds in the perilla leaves, vitamin C and flavonoid compounds in the taro stem segments, so as to ensure that the prepared bee therapy Chinese bee attenuated and desensitized feed can effectively reduce the content of bee venom peptide, phospholipase and hyaluronidase in bee therapy Chinese bees, and reduce the probability of diarrhea in the bees during the breeding process, thereby improving the survival rate of the bees during the breeding process, and then providing healthy bees for bee therapy, to ensure that healthy bees can reliably and stably release bee venom peptide in the body, thereby ensuring the stability of the bee therapy effect.

[0083] The present disclosure also provides a method for raising bee therapy Chinese bees for reducing toxicity and allergy, comprising the following steps: first, the method for preparing the feed for reducing toxicity and allergy of bee therapy described in any of the above embodiments is used to prepare the feed for reducing toxicity and allergy of bee therapy described in any of the above embodiments, so as to ensure that the feed for reducing toxicity and allergy of bee therapy Chinese bees has good effect of reducing toxicity and allergy, good effect of clearing away heat and detoxifying, and less interference with the digestive system of bee therapy Chinese bees; then, the feed for reducing toxicity and allergy of bee therapy Chinese bees is used to feed Chinese bees for 3d to 12d to obtain bee therapy Chinese bees; not only the detoxification effect on Chinese bees is achieved, so as to better adapt to the environment of the Lingnan open-air breeding base which is relatively humid and hot, the survival rate of Chinese bees in the breeding process is effectively improved, and the healthy growth of Chinese bees is effectively guaranteed, thereby improving the survival rate of Chinese bees out of the hive; it also effectively reduces the content of bee venom peptide, phospholipase and hyaluronidase of bee therapy Chinese bees, which not only reduces the pain of patients during bee therapy, but also reduces the degree of allergy of patients after bee therapy, and is better suitable for use in patients with weak constitutions. Furthermore, the feeding amount is 100 g to 300 g per feeding; the feeding time is 16:00 to 18:00 in the evening; the feeding time interval is (24h to 72h) per time, so as to effectively control the feeding amount, time and time interval of feeding, so as to ensure that the Chinese bees are effectively detoxified and desensitized within a shorter feeding time, and that the Chinese bees are not prone to death during the feeding process, thereby improving the survival rate of the bees during the feeding process, and effectively ensuring the healthy growth of the Chinese bees, so as to ensure that the vitality of the Chinese bees after feeding is vigorous, which not only improves the survival rate of the Chinese bees leaving the hive, but also ensures that healthy Chinese bees can reliably and stably release bee venom peptides in the body, thereby ensuring the stability of the apitherapy effect.

[0084] The above-mentioned bee therapy method for reducing toxicity and allergy of Chinese bees is to raise Chinese bees by adopting bee therapy feed for reducing toxicity and allergy of Chinese bees, which has good effect of reducing toxicity and allergy, good effect of clearing away heat and detoxifying, and less interference with the digestive system of Chinese bees, and effectively controls the feeding amount, time and time interval of feeding to ensure that the effect of effectively reducing toxicity and allergy of Chinese bees is achieved within a shorter feeding time, and also ensures that Chinese bees are not prone to death during the feeding process, thereby improving the survival rate of bees during the feeding process, and effectively ensuring the healthy growth of Chinese bees, ensuring that the vitality of Chinese bees after feeding is vigorous, not only improving the survival rate of Chinese bees leaving the hive, but also ensuring that healthy Chinese bees can reliably and stably release bee venom peptides in the body, thereby ensuring the stability of the bee therapy effect.

[0085] See also Figure 2 As shown, the method for preparing the feed for reducing toxicity and allergy of bees in apitherapy in one embodiment includes some or all of the following steps:

[0086] S210. The feed for reducing toxicity and hyposensitization for bees in bee therapy as described in any of the above embodiments is prepared by the method for preparing the feed for reducing toxicity and hyposensitization for bees in bee therapy as described in any of the above embodiments, so as to prepare the feed for reducing toxicity and hyposensitization for bees in bee therapy which has good effect of reducing toxicity and hyposensitization, good efficacy of clearing away heat and detoxifying, and less interference with the digestive system of bees, and set aside.

[0087] S220, feed Chinese bees with the bee therapy Chinese bee detoxification and hyposensitization feed for 3d to 12d to obtain the bee therapy Chinese bees; wherein the feeding amount is 100g to 300g / each time; the feeding time is 16:00 to 18:00 in the evening; the feeding time interval is (24h to 72h) / time.

[0088] It can be understood that by effectively controlling the feeding amount of 100 grams to 300 grams per time, the time of 16:00 to 18:00 in the evening and the time interval of 24h to 72h, the feeding amount of each feeding is ensured to be appropriate, so as to ensure that the Chinese bees are effectively detoxified and desensitized within a shorter feeding time, and it is also ensured that the Chinese bees are not prone to death during the feeding process, thereby improving the survival rate of the bees during the feeding process, and effectively ensuring the healthy growth of the Chinese bees, ensuring that the vitality of the Chinese bees after feeding is vigorous, not only improving the survival rate of the Chinese bees leaving the hive, but also ensuring that healthy Chinese bees can reliably and stably release bee venom peptides in the body, thereby ensuring the stability of the bee therapy effect.

[0089] It can also be understood that if the bees are raised at noon or in the morning when the light is strong, the bee feed for reducing toxicity and hyposensitization of Chinese bees contains vitamin C, isoflavone compounds, and phenolic acid compounds, which are easily decomposed when the light is strong and the temperature is high, resulting in the loss of nutrients in the bee feed for reducing toxicity and hyposensitization of Chinese bees, and the effect of effectively reducing toxicity and hyposensitization of Chinese bees cannot be achieved; therefore, in the present disclosure, by raising Chinese bees at 16:00-18:00 in the evening, on the one hand, the problem of loss of nutrients in the bee feed for reducing toxicity and hyposensitization of Chinese bees due to strong light in the external environment is effectively reduced; on the other hand, since 16:00-18:00 in the evening is a good time for most of the Chinese bees to return to the beehive, most of the Chinese bees can be raised collectively, and the activity intensity of the bees after raising can be ensured to be low, so that the bees can rest after eating, which helps the bees to better absorb the nutrients in the bee feed for reducing toxicity and hyposensitization of Chinese bees, better ensure the healthy growth of the Chinese bees, and help to prepare bee-therapeutic Chinese bees with vigorous vitality.

[0090] In one of the preferred embodiments, the feeding interval is (24h~30h) / time.

[0091] The above-mentioned bee therapy method for reducing toxicity and allergy of Chinese bees is to raise Chinese bees by adopting bee therapy feed for reducing toxicity and allergy of Chinese bees, which has good effect of reducing toxicity and allergy, good effect of clearing away heat and detoxifying, and less interference with the digestive system of Chinese bees, and effectively controls the feeding amount, time and time interval of feeding to ensure that the effect of effectively reducing toxicity and allergy of Chinese bees is achieved within a shorter feeding time, and also ensures that Chinese bees are not prone to death during the feeding process, thereby improving the survival rate of bees during the feeding process, and effectively ensuring the healthy growth of Chinese bees, ensuring that the vitality of Chinese bees after feeding is vigorous, not only improving the survival rate of Chinese bees leaving the hive, but also ensuring that healthy Chinese bees can reliably and stably release bee venom peptides in the body, thereby ensuring the stability of the bee therapy effect.

[0092] In one of the embodiments, the step of feeding the Chinese bees with the apitherapy feed for reducing toxicity and allergy for 3 days to 12 days also includes the following specific steps: delivering the apitherapy feed for reducing toxicity and allergy to Chinese bees to a feeding trough in a beehive through a feeding device, and controlling the temperature in the beehive to 25°C to 38°C, so as to provide a suitable breeding environment for the Chinese bees and help them grow healthily.

[0093] In one of the embodiments, the feeding device includes a brown-black containing bottle, a regulating valve and a brown-black delivery pipe. The brown-black containing bottle is formed with a containing chamber, and the containing chamber is used to hold feed for reducing the toxicity and sensitivity of bees for bee therapy. The brown-black containing bottle is located above a beehive, and a feeding trough is formed in the beehive. The containing chamber of the brown-black containing bottle is connected with the feeding trough through a brown-black delivery pipe. The containing chamber is formed with a feeding port, which is convenient for the operator to throw the feed for reducing the toxicity and sensitivity of bees for bee therapy into the containing chamber from the feeding port, and then the feed for reducing the toxicity and sensitivity of bees for bee therapy in the containing chamber can flow from the brown-black delivery pipe into the feeding trough for the bees to eat; the regulating valve is arranged on the brown-black delivery pipe, and the regulating valve is used to control the opening and closing of the brown-black delivery pipe, so that the regulating valve can effectively control the flow rate of the feed for reducing the toxicity and sensitivity of bees for bee therapy, so as to better meet the feeding amount for different numbers of bees, and there is no need to manually open the box for feeding, which is not only simple to operate but also reduces the risk of stinging, that is, improves the safety of the feeding process.

[0094] It can be understood that the use of the brown-black containing bottle and the brown-black delivery tube can effectively reduce the transmission of light, thereby reducing the loss of nutrients in the bee therapy feed for reducing poison and sensitivity in the delivery process, thereby ensuring that the bee therapy feed for reducing poison and sensitivity in the feeding trough in the beehive has more comprehensive nutrients, which is conducive to the healthy growth of the Chinese bees.

[0095] In one of the embodiments, the brown-black containing bottle is a brown-black polypropylene containing bottle, and the brown-black delivery tube is a brown-black polypropylene delivery tube. Since the air permeability of the brown-black polypropylene containing bottle and the brown-black polypropylene delivery tube is worse than that of polyester plastics, the delivery space formed by the connection of the brown-black polypropylene containing bottle and the brown-black delivery tube has better sealing performance. At the same time, since the brown-black polypropylene containing bottle and the brown-black polypropylene delivery tube have better light-shielding performance, it is ensured that the loss rate of nutrients in the bee therapy feed for reducing toxicity and allergy of Chinese bees delivered to the feeding trough through the brown-black polypropylene containing bottle and the brown-black polypropylene delivery tube is low, which effectively ensures that the bee therapy feed for reducing toxicity and allergy of Chinese bees has better detoxification and allergy-reducing effects, good heat-clearing and detoxifying effects, and less interference with the digestive system of Chinese bees, thereby effectively avoiding the problem of more external air entering, which causes the vitamin C, isoflavone compounds, and phenolic acid compounds in the bee therapy feed for reducing toxicity and allergy of Chinese bees to be easily oxidized, thereby causing the loss of nutrients in the bee therapy feed for reducing toxicity and allergy of Chinese bees.

[0096] In one of the embodiments, after the step of conveying the apitherapy bee attenuated and hypoallergenic feed to the feeding trough in the beehive through the feeding device, when the feeding device finishes conveying the apitherapy bee attenuated and hypoallergenic feed, the brown-black containing bottle is immediately sprayed with clean water through the spray head of the feeding device to remove the apitherapy bee attenuated and hypoallergenic feed adhering to the inner wall of the brown-black containing bottle, and the removed apitherapy bee attenuated and hypoallergenic feed flows through the brown-black polypropylene conveying pipe under the action of gravity to achieve the purpose of removing the apitherapy bee attenuated and hypoallergenic feed adhering to the inner wall of the brown-black polypropylene conveying pipe. The feed for bee therapy with attenuated and desensitized bees can be effectively cleaned, so that the mixed water mixed with the feed for bee therapy with attenuated and desensitized bees can be transported to the feeding trough for water replenishment for the bees, which not only improves the utilization rate of the feed for bee therapy with attenuated and desensitized bees, but also reduces the probability of the feed for bee therapy with attenuated and desensitized bees adhering to the inner wall of the brown-black containing bottle and the brown-black polypropylene delivery tube, reduces the probability of the operator cleaning the feeding device, and effectively avoids the problem of a high probability of cleaning the feeding device due to the brown-black polypropylene delivery tube caused by long-term breeding.

[0097] In one of the embodiments, the brown-black containing bottle, the regulating valve and the brown-black delivery tube are connected to form a delivery assembly similar to an IV drip, which is not only simple to operate and reduces the risk of stinging, but also the regulating valve can better control the delivery speed of the apitherapy Chinese bee detoxification and hyposensitization feed to better meet the feeding amount for different numbers of Chinese bees.

[0098] It is understandable that if the Chinese bees enter the resting stage directly after feeding, the probability of obesity of the Chinese bees will be increased. Therefore, in one embodiment, in the step of feeding the Chinese bees with the feed for reducing toxicity and allergy of the bee therapy Chinese bees for 3d to 12d, the bee therapy Chinese bee reduction and allergy feeding method also includes the following steps: performing lighting operation in the box on the Chinese bees after feeding, and the lighting operation time is 1min to 10min to ensure that the Chinese bees can move in the beehive, effectively reducing the probability of obesity of the Chinese bees, thereby facilitating the preparation of healthy and vigorous bee therapy Chinese bees.

[0099] It can also be understood that if the illumination operation time is less than 1 minute, the probability of obesity of Chinese bees cannot be effectively reduced; if the illumination operation time is less than 10 minutes, the Chinese bees can be more active in the beehive, resulting in a low survival rate of the Chinese bees. Therefore, in the present disclosure, by controlling the illumination operation time to 1 minute to 10 minutes, the activity intensity of the Chinese bees in the beehive is ensured to be more appropriate, which not only reduces the probability of obesity of Chinese bees, but also increases the survival rate of Chinese bees, which is conducive to the preparation of healthy and vigorous bee therapy Chinese bees.

[0100] It is understandable that if the intensity of the light is too strong, the bees will be more active in the beehive, resulting in a lower survival rate of the bees. Therefore, in one embodiment, soft light is used for 10 minutes of lighting operation in the box to ensure that all the bees in the beehive can achieve better activities. Further, the power of the soft light is 1 watt to 10 watts. Further, the power of the soft light is 5 watts.

[0101] In one of the embodiments, a first soft light is arranged on the top of the beehive, a second soft light is arranged on one side of the beehive, and the second soft light is arranged obliquely on a side wall of the beehive to achieve more comprehensive illumination of all the Chinese bees in the beehive, so as to ensure that all the Chinese bees can achieve better activities in the beehive.

[0102] In one embodiment, the first soft light lamp includes a first soft light sub-lamp and a second soft light sub-lamp, and the number of the first soft light sub-lamp and the second soft light sub-lamp is multiple, and a second soft light sub-lamp is arranged between every two adjacent first soft light sub-lamp, and the multiple first soft light sub-lamp and the multiple second soft light sub-lamp are distributed in a matrix on the top of the beehive, and the total illumination area of ​​the multiple first soft light sub-lamp and the multiple second soft light sub-lamp is not less than the area of ​​the longitudinal plane of the beehive, so as to ensure that the multiple first soft light sub-lamp and the multiple second soft light sub-lamp can fully illuminate the longitudinal plane in the beehive. Similarly, since the second soft light lamp includes the third soft light lamp and the fourth soft light lamp, the number of the third soft light lamp and the fourth soft light lamp is multiple, a fourth soft light lamp is arranged between every two adjacent third soft light lamps, and the multiple third soft light lamps and the multiple fourth soft light lamps are distributed in a matrix on one side of the beehive, and the total illumination area of ​​the multiple third soft light lamps and the multiple fourth soft light lamps is not less than the area of ​​the horizontal plane of the beehive, so as to ensure that the multiple third soft light lamps and the multiple fourth soft light lamps can fully illuminate the horizontal plane in the beehive, so as to ensure that all the Chinese bees in the beehive can achieve better activities.

[0103] It should also be noted that, since the present invention can achieve better activities of all the Chinese bees in the beehive by setting soft lights on two sides, there is no need to use soft lights on four sides, which will lead to high light intensity and cause more intense activities of the Chinese bees in the beehive, resulting in a lower survival rate of the Chinese bees; and compared with soft lights set on three or four sides, the two sides of the present invention can effectively reduce the cost of soft lights.

[0104] It is understandable that since Chinese bees are phototropic animals, when the first soft light or the second soft light is turned on, some Chinese bees will gather on the first soft light and the second soft light, which will affect the physiological health of the Chinese bees and cannot ensure the preparation of healthy and vigorous apitherapy Chinese bees. Therefore, in the present disclosure, the step of performing lighting operation on the Chinese bees after feeding includes the following specific steps: first, start all the first soft light lamps, all the second soft light lamps, all the third soft light lamps, and all the fourth soft light lamps for lighting for 20S to 50S to ensure that all the Chinese bees in the beehive are active; then, turn off all the second soft light lamps and all the fourth soft light lamps, keep all the first soft light lamps and all the fourth soft light lamps for lighting for 30S to 50S, then turn off all the first soft light lamps and all the fourth soft light lamps again, turn on all the first soft light lamps and all the fourth soft light lamps for lighting for 10S to 30S, and finally, turn off all the first soft light lamps, second soft light lamps, third soft light lamps, and fourth soft light lamps. In this way, while minimizing the physiological health of the Chinese bees, it also ensures that all the Chinese bees in the beehive can carry out more appropriate activities, thereby improving the survival rate of Chinese bee breeding.

[0105] In one embodiment, honeysuckle, wild chrysanthemum, perilla leaf, kudzu flower and liquorice are all obtained by drying.

[0106] In one embodiment, after the Chinese bees are raised for 3 to 12 days using the bee therapy Chinese bee detoxification and hypoallergenic feed disclosed in the present invention, the content of bee venom peptide in the bee therapy Chinese bees is 35.00% to 45.00%; the content of phospholipase in the bee therapy Chinese bees is 9.00% to 13%; the content of hyaluronidase in the bee therapy Chinese bees is 1.00% to 3.00%, so as to achieve a better detoxification and hypoallergenic effect; and the survival rate of the Chinese bees in the breeding process is improved, that is, the 3-day survival rate of the Chinese bees is 80% to 95%; the 7-day survival rate of the Chinese bees is 80% to 90%; the survival rate of the Chinese bees out of the hive is improved, that is, the survival rate of the bee therapy Chinese bees out of the hive for 3 days is 70% to 80%; the survival rate of the bee therapy Chinese bees out of the hive for 7 days is 60% to 70%.

[0107] Some specific examples are given below, and if % is mentioned, it means percentage by weight. It should be noted that the following examples do not exhaust all possible situations, and the materials used in the following examples can be obtained from commercial sources unless otherwise specified.

[0108] Among them, honeysuckle is provided by Lingnan Chinese herbal medicine manufacturers, wild chrysanthemum is provided by Lingnan Chinese herbal medicine manufacturers, taro stem segments are provided by Kangmei Pharmaceutical, perilla leaves are provided by Lingnan Chinese herbal medicine manufacturers, kudzu flowers are provided by Kangmei Pharmaceutical, licorice is provided by Lingnan Chinese herbal medicine manufacturers, and honey is provided by Wuhan Fengzhichao Bioengineering Co., Ltd.

[0109] Table 1 Recipe

[0110]

[0111] A method for raising bees for reducing toxicity and allergy in apitherapy, comprising the following steps:

[0112] Step 1: Preparation of taro stem segments

[0113] S11, obtaining fresh, complete and mature taro stem strips;

[0114] S12, washing the taro stem strips 3 times, removing 1 / 3 of the taro stem segment closer to the taro,

[0115] S13, the remaining 2 / 3 of the taro stems are punched, so that a plurality of through holes are distributed in a matrix in the length direction of the taro stems, and the spacing between each through hole is 0.2 cm;

[0116] S14, immediately immersing the remaining 2 / 3 of the taro stems that have completed the hole-piercing operation completely in clean water;

[0117] S15, take out 2 / 3 of the taro stem strips, and cut into sections along a plurality of longitudinally straight through holes to obtain fresh taro stem sections of 3 cm in length for standby use;

[0118] Step 2: Fry over high heat until the water boils for 2 minutes, remove the processed taro stems and set aside;

[0119] Step 3: Add honeysuckle, wild chrysanthemum, kudzu flower and liquorice to water according to the formula in Table 1, first boil the water with high heat until the water boils, then boil it with low heat for 20 minutes to complete a decoction operation to obtain Chinese medicine mixed water A, wherein 500 ml of water is added for every 200 grams of the main raw material;

[0120] Step 3: Add perilla leaves and processed taro stem segments to the Chinese medicine mixed water A, first decoct with high heat until the water boils, then decoct with medium heat for 10 minutes to complete a decocting operation, and obtain Chinese medicine mixed water B.

[0121] Step 4: filtering the Chinese medicine mixed water to obtain Chinese medicine water;

[0122] Step 5: Evenly mix the Chinese medicinal water and honey in a weight ratio of 1:1.2 to obtain a feed for reducing toxicity and allergy for bee therapy, for later use;

[0123] Step 6: At 17:00 in the evening, pour the bee therapy Chinese bee detoxification and hypoallergenic feed prepared in step 5 into a brown-black polypropylene container bottle, and then adjust the delivery speed through the regulating valve to deliver the bee therapy Chinese bee detoxification and hypoallergenic feed to the feeding trough for use by the Chinese bees; wherein, the feeding amount of the bee therapy Chinese bee detoxification and hypoallergenic feed poured each time is 200 grams, the time interval is 24 hours, and the temperature in the beehive is controlled at 25°C to 38°C; wherein, the breeding place of the Chinese bees is Huizhou First People's Hospital, please refer to Figure 3 .

[0124] Step 7: After the feeding material for reducing toxicity and allergy for bees in the brown-black polypropylene container bottle is delivered, the spray head of the feeding device is immediately started to spray the brown-black container bottle with clean water, so that the washed mixed water mixed with the feeding material for reducing toxicity and allergy for bees can be delivered to the feeding trough to supplement the water source for the bees;

[0125] Step 8: After 10 minutes of completing step 7, perform the lighting operation;

[0126] S81, start all the first soft light lamps (5 watts), all the second soft light lamps (5 watts), all the third soft light lamps (5 watts), and all the fourth soft light lamps (5 watts) in the beehive for illumination for 30 seconds;

[0127] S82, turn off all the second soft light sub-lights and all the fourth soft light sub-lights, and keep all the first soft light sub-lights and all the fourth soft light sub-lights illuminating for 40 seconds;

[0128] S83, turn off all the first soft light sub-lights and all the fourth soft light sub-lights, and turn on all the first soft light sub-lights and all the fourth soft light sub-lights to illuminate for 30 seconds;

[0129] S84, turning off all the first soft light, the second soft light, the third soft light, and the fourth soft light;

[0130] Step 9: After completing step 8, the Chinese bee enters the sleep stage;

[0131] Step 10: At dawn the next day, the Chinese bees get up and move around. At 17:00 in the evening of the next day, repeat steps 6 to 9 to complete the feeding for the next day.

[0132] Step 11: Follow the operation of step 10 to continuously raise the Chinese bees for 12 days to obtain the apitherapy Chinese bees of Examples 1 to 3 and Comparative Examples 1 to 3.

[0133] Example 4

[0134] The difference from Example 1 is that the feeding amount of 200 grams in step 6 of Example 1 is directly replaced with a feeding amount of 100 grams, and the rest remains unchanged.

[0135] Example 5

[0136] The difference from Example 1 is that the feeding amount of 200 grams in step 6 of Example 1 is directly replaced with a feeding amount of 300 grams, and the rest remains unchanged.

[0137] Example 6

[0138] The difference from Example 1 is that the feeding time interval of 24 hours in step 6 of Example 1 is directly replaced with a feeding time interval of 48 hours, and the rest remains unchanged.

[0139] Example 7

[0140] The difference from Example 1 is that the feeding time interval of 24 hours in step 6 of Example 1 is directly replaced with a feeding time interval of 72 hours, and the rest remains unchanged.

[0141] Example 8

[0142] The difference from Example 1 is that the temperature of the beehive during feeding, which is 25° C., in step 6 of Example 1 is directly replaced with the temperature of the beehive during feeding, which is 30° C., and the rest remain unchanged.

[0143] Example 9

[0144] The difference from Example 1 is that the temperature of the beehive during feeding, which is 25° C., in step 6 of Example 1 is directly replaced with the temperature of the beehive during feeding, which is 38° C., and the rest remain unchanged.

[0145] Comparative Example 4

[0146] The difference from Example 1 is that the bees were not raised with feed for reducing toxicity and allergy in apitherapy, that is, they were naturally cultured.

[0147] Comparative Example 5

[0148] The difference from Example 1 is that the feeding amount of 100 grams in step 6 of Example 1 is directly replaced with a feeding amount of 80 grams, and the rest remains unchanged.

[0149] Comparative Example 6

[0150] The difference from Example 1 is that the feeding amount of 100 grams in step 6 of Example 1 is directly replaced with a feeding amount of 350 grams, and the rest remains unchanged.

[0151] Comparative Example 7

[0152] The difference from Example 1 is that the feeding time interval of 24 hours in step 6 of Example 1 is directly replaced with a feeding time interval of 20 hours, and the rest remains unchanged.

[0153] Comparative Example 8

[0154] The difference from Example 1 is that the feeding time interval of 24 hours in step 6 of Example 1 is directly replaced with a feeding time interval of 75 hours, and the rest remains unchanged.

[0155] Comparative Example 9

[0156] The difference from Example 1 is that the temperature of the beehive during feeding, which is 25° C., in step 6 of Example 1 is directly replaced with the temperature of the beehive during feeding, which is 20° C., and the rest remain unchanged.

[0157] Comparative Example 10

[0158] The difference from Example 1 is that the temperature of the beehive during feeding, which is 25° C., in step 6 of Example 1 is directly replaced with the temperature of the beehive during feeding, which is 40° C., and the rest remain unchanged.

[0159] Comparative Example 11

[0160] The difference from Example 1 is that, unlike step 8 of Example 1, comparative example 11 directly adopts the S81 mode for irradiation for 100S, and the rest remains unchanged.

[0161] Comparative Example 12

[0162] The difference from Example 1 is that the step S13 is omitted from the step 1 of Example 1, and the rest remains unchanged.

[0163] The bee venom peptide, phospholipase, hyaluronidase, 3-day survival rate and 7-day survival rate of the bee venom prepared in the above Examples 1 to 9 and Comparative Examples 1 to 12 were tested to obtain the experimental data in Table 2 below:

[0164] Detection method of bee venom peptide: liquid chromatography tandem mass spectrometry detection.

[0165] Detection method of phospholipase: spectrophotometry / microassay detection.

[0166] Detection method of hyaluronidase: ELISA detection.

[0167] Method for detecting the survival rate after 3 days of breeding: calculated based on the number of dead bees around the beehive.

[0168] The method for testing the survival rate of bees after 7 days of feeding is calculated based on the number of dead bees around the beehive. The method for testing the survival rate after 3 days of leaving the hive is calculated based on the number of surviving bees in the storage box. The method for testing the survival rate after 7 days of leaving the hive is calculated based on the number of surviving bees in the storage box.

[0169] Table 2 Experimental data

[0170]

[0171]

[0172] It can be seen from the data in Table 2 above that after the Chinese bees are regularly raised with the feed for reducing toxicity and allergy of the apitherapy Chinese bees disclosed in the present invention, not only can the content of bee venom peptide, phospholipase and hyaluronidase of the apitherapy Chinese bees be effectively reduced, thereby reducing the pain of the patients during apitherapy, but also reducing the degree of allergy of the patients after apitherapy; it also helps to improve the survival rate of the apitherapy Chinese bees out of the box and the survival rate of the Chinese bees during the breeding process, such as Figure 4 As shown, the cost of apitherapy is effectively reduced. Among them, the comprehensive index of embodiment 1 is the best.

[0173] The above-mentioned embodiments only express several implementation methods of the present disclosure, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the disclosed patent. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present disclosure, and these all belong to the protection scope of the present disclosure. Therefore, the protection scope of the disclosed patent shall be subject to the attached claims.

Claims

1. A feed for reducing toxicity and allergy of bees in apitherapy, characterized in that: It includes the following components by weight:

2. A method for preparing a feed for reducing toxicity and allergy of bees in apitherapy, characterized in that: The steps include: blanching the fresh taro stem segments to obtain processed taro stem segments for later use; Add honeysuckle, wild chrysanthemum, kudzu flower and liquorice into water for decoction to obtain Chinese medicine mixed water A; Adding perilla leaves and the processed taro stem segments to the Chinese medicine mixed water A for secondary decoction to obtain Chinese medicine mixed water B; Filtering the Chinese medicine mixed water B to obtain Chinese medicine water; The Chinese medicinal water is mixed with honey to obtain the bee therapy feed for reducing toxicity and allergy of Chinese bees as claimed in claim 1.

3. The method for preparing the feed for reducing toxicity and allergy of bees in apitherapy according to claim 2, wherein the blanching operation comprises the following specific steps: Use high heat to simmer until the water boils for 2min to 5min.

4. The method for preparing the feed for reducing toxicity and allergy of bees in apitherapy according to claim 2, wherein the one-time decoction operation comprises the following specific steps: First use high heat to boil until the water boils, then switch to low heat and simmer for 20 to 40 minutes.

5. The method for preparing the feed for reducing toxicity and allergy of bees in apitherapy according to claim 2, wherein the secondary decoction operation comprises the following specific steps: First use high heat to boil until the water boils, then switch to medium heat and simmer for 5 to 20 minutes.

6. The method for preparing the feed for reducing toxicity and allergy of bees in apitherapy according to claim 2, characterized in that: The amount of water used is 500 ml of water for every 200 grams of the main raw material.

7. The method for preparing the feed for reducing toxicity and allergy of bees in apitherapy according to claim 2, characterized in that: The weight ratio of the Chinese medicinal water to the honey is 1:(1-1.5).

8. A method for raising bees for reducing toxicity and allergy in apitherapy, characterized in that: The steps include: The feed for reducing toxicity and allergy of bees in apitherapy according to claim 1 is prepared by the method for preparing the feed for reducing toxicity and allergy of bees in apitherapy according to any one of claims 2 to 7; The bee therapy Chinese bee attenuated and hypoallergenic feed is used to feed the bee for 3 to 12 days to obtain the bee therapy Chinese bee; The feeding amount is 100 g to 300 g per feeding; the feeding time is 16:00 to 18:00 in the evening; and the feeding time interval is (24h to 72h) per feeding.

9. The method for raising bees for reducing toxicity and allergy according to claim 8, characterized in that: The step of feeding the Chinese bees with the apitherapy feed for reducing toxicity and allergy for 3 to 12 days also includes the following specific steps: The feed for reducing the toxicity and sensitivity of bees in apitherapy is transported to a feeding trough in a beehive through a feeding device, and the temperature in the beehive is controlled to be 25° C. to 38° C.

10. The method for raising bees for reducing toxicity and allergy according to claim 8, characterized in that: In the step of feeding the Chinese bees with the apitherapy Chinese bee detoxification and hypoallergenic feed for 3d to 12d, the apitherapy Chinese bee detoxification and hypoallergenic feeding method also includes the following steps: The feeding Chinese bees are subjected to lighting operation in the box, and the lighting operation time is 1min to 10min.

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