Collagen composition with anti-wrinkle and repairing effects and preparation method thereof

Abstract

The invention relates to the technical field of skin care, in particular to a collagen composition with anti-wrinkle and repairing effects and a preparation method. Comprising 1.5-4.5 parts of mixed collagen, 2.5-5 parts of mixed polypeptide and 4-8 parts of mixed extract, wherein the mixed extract comprises 1-2 parts of centella asiatica extract, 1-2 parts of polygonum cuspidatum root extract, 1-2 parts of bilberry fruit extract and 1-2 parts of bidens pilosa extract; the collagen is directly supplied to the skin to supplement collagen, and is mainly used for repairing the skin structure, increasing the density, elasticity and compactness of the skin and remarkably improving fine wrinkles and looseness; the polypeptide inhibits the expression of MMP-1; the natural plant active ingredients promote the expression of TGF-beta, enhance the activity of fibroblasts, accelerate the synthesis of collagen, prevent excessive oxidation of skin, repair skin inflammation, activate cell energy and delay skin aging.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care, in particular to a collagen composition with anti-wrinkle and repairing effects and a preparation method thereof. Background Art

[0002] In the prior art, collagen, peptides, Centella asiatica leaf extracts and Polygonum cuspidatum root extracts can be used to achieve anti-aging, anti-wrinkle and repair of the skin. For example, China's patent for an anti-allergic repair composition and its preparation method, patent number 201910899192.7, which records an anti-allergic repair composition and its preparation method, the composition comprises hydrolyzed collagen, peptides, fullerenes and anti-allergic and anti-inflammatory ingredients; the anti-allergic and anti-inflammatory ingredients are extracts of chamomile, Centella asiatica leaves, Polygonum cuspidatum roots, Dendrobium officinale stems and Scutellaria baicalensis roots, as well as one or more of glycyrrhizic acid and its derivatives, bisabolol, menthol, allantoin, and nano silver ions. Hydrolyzed collagen has a strong moisturizing ability, peptides can promote skin elasticity, fullerenes combined with peptides can eliminate active oxygen, have a long antioxidant time, can effectively reduce melanin synthesis, promote collagen synthesis, have anti-inflammatory whitening and repair sunburned skin effects, and the active ingredients in the anti-allergic and anti-inflammatory ingredients repair damaged skin cells while moisturizing and hydrating, restoring the skin to a hydrated state.

[0003] The disadvantage of the prior art is that although the technical solution claims to have the effect of repairing damaged skin cells, it is found in practice that the repair effect of the technical solution is not significant. An anti-wrinkle product with better repair effect is urgently needed. Summary of the invention

[0004] In view of the deficiencies in the prior art, one of the objects of the present invention is to provide a collagen composition with anti-wrinkle and repairing effects; a second object of the present invention is to provide a method for preparing the collagen composition with anti-wrinkle and repairing effects; a third object of the present invention is to provide a use of the collagen composition with anti-wrinkle and repairing effects.

[0005] One of the purposes of the present invention is achieved through the following technical solutions:

[0006] A collagen composition with anti-wrinkle and repairing effects, comprising the following components in parts by weight:

[0007] 1.5-4.5 parts of mixed collagen, 2.5-5 parts of mixed peptides and 4-8 parts of mixed extracts;

[0008] The mixed extract comprises: 1-2 parts of Centella asiatica extract, 1-2 parts of Polygonum cuspidatum root extract, 1-2 parts of Vaccinium myrtillus fruit extract (also known as: Vaccinium myrtillus fruit extract) and 1-2 parts of Bidens pilosa extract.

[0009] Furthermore, the mixed collagen includes: 0.5-1.5 parts of type I collagen, 0.5-1.5 parts of type III collagen and 0.5-1.5 parts of type IV collagen.

[0010] Furthermore, the mixed polypeptide comprises: 0.5-1 part of acetyl tetrapeptide-2, 0.5-1 part of glutathione, 0.5-1 part of carnosine, 0.5-1 part of palmitoyl tripeptide-8 and 0.5-1 part of palmitoyl tripeptide-5.

[0011] In a preferred embodiment, the mass ratio of type I collagen, type III collagen and type IV collagen in the mixed collagen is 1:3:3.

[0012] In a preferred embodiment, the mass ratio of acetyl tetrapeptide-2, glutathione, carnosine, palmitoyl tripeptide-8 and palmitoyl tripeptide-5 in the mixed polypeptide is 2:1:2:1:1.

[0013] In a preferred embodiment, the resveratrol content in the Polygonum cuspidatum root extract is ≥5wt%; currently, there are multiple concentration specifications for this raw material, but the Polygonum cuspidatum root extract with a resveratrol content of about 5wt% has a better effect. Resveratrol is a polyphenol substance, and its main source plants are Polygonum cuspidatum and grapes. Resveratrol has a strong antioxidant capacity, can effectively inhibit the occurrence of oxidative reactions in the human body, and can remove free radicals and lipid peroxides in the human body, helping to maintain and delay aging. Free radicals have the effect of promoting inflammatory responses, and resveratrol can remove free radicals through antioxidant effects, block the positive feedback loop of oxidative stress and pro-inflammatory cytokines, and thus achieve the purpose of anti-inflammatory.

[0014] In a preferred embodiment, the pterostilbene content in the blueberry extract is ≥ 0.3 wt %; it is known that the pterostilbene content of blueberry extract can reach 0.5 wt % through the selection of raw materials and pretreatment, but experiments have found that high concentrations do not continue to improve the efficacy. Pterostilbene has multiple skin care effects such as anti-oxidation, anti-inflammatory, whitening, preventing UV damage, preventing and treating skin diseases.

[0015] The second object of the present invention is achieved by the following technical solutions:

[0016] A method for preparing a collagen composition having anti-wrinkle and repairing effects comprises the following steps:

[0017] Step 1, weigh the raw materials according to the formula;

[0018] Step 2, taking an appropriate amount of deionized water to dissolve the mixed collagen to obtain solution a;

[0019] Step 3, taking an appropriate amount of deionized water to dissolve the mixed polypeptide to obtain solution b;

[0020] Step 4, combining solution a and solution b, heating to 50°C and keeping warm for 1-2 hours to obtain solution c;

[0021] Step 5: Add the mixed extract into solution c and stir to dissolve, then cool to room temperature and dilute with deionized water to a total mass concentration of 8-17.5wt%, to obtain a collagen composition with anti-wrinkle and repair effects.

[0022] The third object of the present invention is achieved by the following technical solutions:

[0023] The invention discloses a collagen composition having anti-wrinkle and repairing effects and its use in preparing skin care products.

[0024] The invention discloses a collagen composition having anti-wrinkle and repairing effects and its use in preparing cosmetics.

[0025] The invention discloses a collagen composition having anti-wrinkle and repairing effects and its use in preparing medical dressings.

[0026] The beneficial effects of the present invention are:

[0027] First, triple gene recombinant humanized collagen directly supplements collagen to the skin, mainly acting on skin structure repair, increasing skin density, elasticity and firmness, and significantly improving fine lines and sagging. It can enhance the overall firmness of the skin, restore the skin to a youthful state, and improve the appearance and texture of the skin. It enhances the stability and structure of the skin basement membrane, optimizes the combination of collagen and skin cells, and improves the firmness and anti-aging ability of the skin. It enhances the toughness and elasticity of the skin, improves the firmness of cells, and plays an important role in the healing process of skin damage or trauma.

[0028] Second, the five-peptide collagen protection mechanism inhibits the expression of MMP-1 (matrix metalloproteinase), prevents collagen degradation, and activates DNA repair.

[0029] Third, multiple natural plant active ingredients promote the expression of TGF-β, enhance the activity of fibroblasts, accelerate the synthesis of collagen, prevent excessive oxidation of the skin, repair skin inflammation, activate cell energy, and delay skin aging; TGF-β is an important factor in promoting collagen synthesis, which can activate fibroblasts and stimulate the production of collagen. TGF-β not only regulates the synthesis of collagen, but also plays an important role in the skin repair process. DETAILED DESCRIPTION

[0030] The following is further described in conjunction with specific implementation methods:

[0031] The raw material formulas of each embodiment and comparative example are shown in Table 1 (by weight). Unless otherwise specified, all other raw materials are purchased from conventional sources. The resveratrol content of the purchased Polygonum cuspidatum root extract is 5wt%; the pterostilbene content of the purchased Vaccinium myrtillus fruit extract is 0.3wt%.

[0032] Table 1 Recipe

[0033]

[0034] Example 1

[0035] A method for preparing a collagen composition having anti-wrinkle and repairing effects comprises the following steps:

[0036] Step 1, weigh the raw materials according to the formula;

[0037] Step 2, taking 40 parts of deionized water to dissolve the mixed collagen to obtain solution a;

[0038] Step 3, taking 40 parts of deionized water to dissolve the mixed polypeptide to obtain solution b;

[0039] Step 4, combining solution a and solution b, heating to 50°C and keeping warm for 1 hour to obtain solution c;

[0040] Step 5: Add the mixed extract into solution c and stir to dissolve, then cool to room temperature and dilute to a total mass concentration of 8 wt % with deionized water to obtain a collagen composition with anti-wrinkle and repair effects.

[0041] Example 2

[0042] A method for preparing a collagen composition having anti-wrinkle and repairing effects comprises the following steps:

[0043] Step 1, weigh the raw materials according to the formula;

[0044] Step 2, taking 40 parts of deionized water to dissolve the mixed collagen to obtain solution a;

[0045] Step 3, taking 40 parts of deionized water to dissolve the mixed polypeptide to obtain solution b;

[0046] Step 4, combining solution a and solution b, heating to 50°C and keeping warm for 2 hours to obtain solution c;

[0047] Step 5: Add the mixed extract into solution c and stir to dissolve, then cool to room temperature and dilute to a total mass concentration of 17.5 wt % with deionized water to obtain a collagen composition with anti-wrinkle and repair effects.

[0048] Example 3

[0049] A method for preparing a collagen composition having anti-wrinkle and repairing effects comprises the following steps:

[0050] Step 1, weigh the raw materials according to the formula;

[0051] Step 2, taking 40 parts of deionized water to dissolve the mixed collagen to obtain solution a;

[0052] Step 3, taking 40 parts of deionized water to dissolve the mixed polypeptide to obtain solution b;

[0053] Step 4, combining solution a and solution b, heating to 50° C. and keeping the temperature for 1.5 h to obtain solution c;

[0054] Step 5: Add the mixed extract into solution c and stir to dissolve, then cool to room temperature and dilute to a total mass concentration of 12.5 wt % using deionized water to obtain a collagen composition with anti-wrinkle and repair effects.

[0055] Example 4

[0056] The blueberry extract of the present embodiment is homemade, and the homemade method is as follows:

[0057] First, wash the blueberry fruit (fruit of Vaccinium vitis-idaea L.), dry the surface in the shade, and then place it under artificial light for illumination treatment. The illumination conditions are: a mixture of blue light and red light in a ratio of 1:3, the illumination time is: 50h, the temperature is 15℃, the relative humidity is 55%, and the wind speed is 0.3m / s.

[0058] Then the blueberry fruit is crushed to make pulp, and then the pulp residue is separated;

[0059] The slurry is heated; filtered with a 0.35 micron filter membrane to obtain a filtrate; the filtrate is concentrated under reduced pressure, and the solvent is recovered to obtain a concentrate; the concentrate is rinsed with petroleum ether, then dissolved with an ethanol solution, ultrafiltered with a filter membrane, and treated with an adsorption resin after ultrafiltration to obtain an eluate; the eluate is concentrated, the solvent is recovered, and an extract is obtained, and the extract is dissolved and filtered with methanol; material A is obtained;

[0060] The separated residue is extracted by ultra-high pressure extraction equipment with ethanol as solvent, and the mass fraction concentration of ethanol is 70%; the obtained extract is concentrated to obtain a concentrate, and the concentrate is dissolved and filtered with n-hexane to obtain a filter cake; the filter cake is dissolved and filtered with methanol, and the filtrate is collected to obtain material B.

[0061] The materials A and B are combined and then dried at low temperature to form a powder with a water content of 9% to obtain the blueberry fruit extract.

[0062] A method for preparing a collagen composition having anti-wrinkle and repairing effects comprises the following steps:

[0063] Step 1, weigh the raw materials according to the formula;

[0064] Step 2, taking 40 parts of deionized water to dissolve the mixed collagen to obtain solution a;

[0065] Step 3, taking 40 parts of deionized water to dissolve the mixed polypeptide to obtain solution b;

[0066] Step 4, combining solution a and solution b, heating to 50° C. and keeping the temperature for 1.5 h to obtain solution c;

[0067] Step 5: Add the mixed extract into solution c and stir to dissolve, then cool to room temperature and dilute to a total mass concentration of 13 wt % with deionized water to obtain a collagen composition with anti-wrinkle and repair effects.

[0068] Comparative Example 1

[0069] The steps are the same as those in Example 3.

[0070] Comparative Example 2

[0071] The steps are the same as those in Example 3.

[0072] Comparative Example 3

[0073] The steps are the same as those in Example 3.

[0074] MMP-1 inhibition rate experiment

[0075] HaCaT cells were inoculated in a 96-well plate and cultured to a density of 80%. A blank group, a model group, and an experimental group were set up, and 6 replicate wells were set up in each group to take the average. The blank group was not irradiated with UVB, and 10 μL of culture solution was added for 2 hours, and then cultured at 37°C for 24 hours before being used in the experiment. The HaCaT cells in the model group were treated with 10 μL of culture solution for 2 hours, and then irradiated with 60 mJ / cm2 of UVB, and cultured at 37°C for 24 hours before being used in the experiment. The HaCaT cells in the experimental group were treated with 10 μL of culture solution (composition content 0.5 mg / mL) for 2 hours, and then irradiated with 60 mJ / cm2 of UVB, and cultured at 37°C for 24 hours before being used in the experiment. Centrifuge for 10 minutes at 4°C and 12000 r / min, and collect the supernatant. The protein in the supernatant was quantified using a BCA protein quantification kit, and the final concentration was determined to be 10 μg / mL. The expression level of the secretory protein MMP-1 was determined according to the instructions of the enzyme-linked immunosorbent assay (ELISA) kit. The inhibition rate of MMP-1 (matrix metalloproteinase) was calculated.

[0076] The experimental results are shown in Table 1.

[0077] Table 1 Statistical table of the inhibition rate of each sample on MMP-1

[0078]

[0079] Experiments show that the composition obtained by the present invention can effectively inhibit the secretion of MMP-1, thereby improving the anti-wrinkle and repair effects. Although the comparative examples 1 and 2 can also achieve certain effects when using a single collagen and polypeptide, there is still room for improvement. In addition, when the comparative example 3 uses a single plant extract, its efficacy is greatly reduced due to the lack of synergistic effect.

[0080] Fibroblast expression assay

[0081] 3T3 mouse fibroblasts were diluted with cell culture medium and cultured to a density of 50%, inoculated into 96-well plates, with a liquid volume of 200 μL per well, and placed in a CO2 incubator for 24 hours. The culture medium in the 96-well plate was discarded and the drug administration operation was carried out. The culture medium containing the composition was added to the test wells (the content of the composition in the culture medium was 1.25 mg / mL), and normal cell culture medium was added to the blank / solvent control wells, 200 μL per well. After the administration, the 96-well plate was placed in a CO2 incubator for 24 hours. After the incubation and culture, 200 μL of cell culture supernatant was collected in a 1.5 mL sterile centrifuge tube and placed in a -80°C ultra-low temperature refrigerator for freezing. The detection was performed according to the instructions of the mouse type I collagen enzyme-linked immunosorbent assay kit. The upregulation rate of type I collagen expression was calculated.

[0082] The results are shown in Table 2.

[0083] Table 2 Statistics of up-regulation rate of fibroblast expression

[0084]

[0085] Experiments show that Examples 1-4 and Comparative Examples 1-2 can achieve excellent effects in promoting collagen expression, but the efficacy of Comparative Example 3 is greatly reduced due to the lack of various plant extracts.

[0086] The above embodiments and descriptions are only for illustrating the principles and best embodiments of the present invention. Without departing from the spirit and scope of the present invention, the present invention may be subject to various changes and improvements, all of which fall within the scope of the present invention to be protected.

Claims

1. A collagen composition with anti-wrinkle and repairing effects, characterized in that: The composition comprises the following components in parts by weight: 1.5-4.5 parts of mixed collagen, 2.5-5 parts of mixed peptides and 4-8 parts of mixed extracts; The mixed extract comprises: 1-2 parts of Centella asiatica extract, 1-2 parts of Polygonum cuspidatum root extract, 1-2 parts of Vaccinium myrtillus fruit extract and 1-2 parts of Bidens pilosa L. extract.

2. The collagen composition with anti-wrinkle and repairing effects according to claim 1, characterized in that: The mixed collagen comprises: 0.5-1.5 parts of type I collagen, 0.5-1.5 parts of type III collagen and 0.5-1.5 parts of type IV collagen.

3. The collagen composition with anti-wrinkle and repairing effects according to claim 1, characterized in that: The mixed polypeptide comprises: 0.5-1 part of acetyl tetrapeptide-2, 0.5-1 part of glutathione, 0.5-1 part of carnosine, 0.5-1 part of palmitoyl tripeptide-8 and 0.5-1 part of palmitoyl tripeptide-5.

4. The collagen composition with anti-wrinkle and repairing effects according to claim 1, characterized in that: The mass ratio of type I collagen, type III collagen and type IV collagen in the mixed collagen is 1:3:

3.

5. The collagen composition with anti-wrinkle and repairing effects according to claim 1, characterized in that: The mass ratio of acetyl tetrapeptide-2, glutathione, carnosine, palmitoyl tripeptide-8 and palmitoyl tripeptide-5 in the mixed polypeptide is 2:1:2:1:

1.

6. The collagen composition with anti-wrinkle and repairing effects according to claim 1, characterized in that: The resveratrol content in the Polygonum cuspidatum root extract is ≥5wt%.

7. The collagen composition with anti-wrinkle and repairing effects according to claim 1, characterized in that: The pterostilbene content in the blueberry fruit extract is ≥0.3 wt %.

8. A method for preparing the collagen composition according to any one of claims 1 to 7, characterized in that: The following steps are involved: Step 1, weigh the raw materials according to the formula; Step 2, dissolving the mixed collagen in deionized water to obtain solution a; Step 3, dissolving the mixed polypeptide in deionized water to obtain solution b; Step 4, combining solution a and solution b, heating to 50°C and keeping warm for 1-2 hours to obtain solution c; Step 5: Add the mixed extract into solution c and stir to dissolve, then cool to room temperature and dilute with deionized water to a total mass concentration of 8-17.5wt%, to obtain a collagen composition with anti-wrinkle and repair effects.

9. Use of the collagen composition according to claim 8 in preparing skin care products or cosmetics.

10. Use of the collagen composition according to claim 8 in preparing medical dressings.

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