Composite plant extract as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to a composite plant extract as well as a preparation method and application thereof. The composite plant extract is prepared from the following raw materials in percentage by mass: 0.5 to 1 percent of herba verbenae extract, 4 to 6 percent of herba centellae extract, 1.5 to 2.5 percent of rhizoma polygoni cuspidati root extract, 1.5 to 2.5 percent of radix scutellariae root extract, 0.5 to 1.5 percent of tea leaf extract, 0.5 to 1.5 percent of glycyrrhiza glabra extract, 0.1 to 1.0 percent of chamomilla recutita flower extract, 0.1 to 1.0 percent of rosemary leaf extract, 40 to 60 percent of butanediol and the balance of water. In the extraction process of the verbena extract, sodium selenite, gamma-oryzanol and other components are innovatively added into the culture medium, and the content of total flavonoids in the verbena extract can be effectively increased through the synergistic effect of sodium selenite and gamma-oryzanol. Furthermore, the total flavonoid content in the verbena extract is increased, so that the skin repairing barrier effect of the cosmetic can be effectively enhanced.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composite plant extract and a preparation method and application thereof. Background Art

[0002] The skin barrier, also known as the protective layer of the skin, is mainly composed of the stratum corneum, sebum membrane and related skin structures. It plays a vital role in maintaining skin health, preventing the invasion of harmful substances from the outside world and maintaining the moisture balance of the skin. Modern people are prone to skin barrier damage due to long-term high pressure, long-term exposure to sunlight, frequent use of overly irritating cosmetics, excessive cleaning, etc. When the skin barrier is damaged, the skin will have a series of problems, such as redness, peeling, dryness, itching, etc., and in severe cases, it may even cause skin inflammation or infection.

[0003] Substances such as flavonoids, organic acids, and amino acids contained in plants can produce different effects and influences on human skin. For example, flavonoids have significant effects in resisting skin peroxidation, delaying aging, and whitening, while amino acids can reduce wrinkles. Therefore, plant extract cosmetics have attracted much attention. Flavonoids are one of the important active ingredients in verbena, and have multiple biological activities, such as antioxidant, anti-inflammatory, and antibacterial. In the fields of cosmetics, health products, and pharmaceuticals, verbena extracts have attracted much attention due to their rich flavonoids, but the total flavonoid content in verbena extracts will be affected by the extraction method, thus limiting their application in various fields.

[0004] Based on the above background, it is necessary to improve the extraction method of verbena extract to increase the total flavonoids content in verbena extract, thereby accelerating its application in various fields. Summary of the invention

[0005] In order to overcome the deficiencies of the prior art, one of the purposes of the present invention is to provide a composite plant extract having an excellent skin barrier repairing effect.

[0006] The second object of the present invention is to provide a method for preparing a composite plant extract, which has simple steps and is easy to operate.

[0007] The third object of the present invention is to provide an application of a composite plant extract, which has broad application prospects.

[0008] One of the purposes of the present invention is achieved by the following technical solution:

[0009] A composite plant extract comprises the following raw materials in percentage by weight: 0.5-1% of verbena extract, 4-6% of centella asiatica extract, 1.5-2.5% of polygonum cuspidatum root extract, 1.5-2.5% of scutellaria baicalensis root extract, 0.5-1.5% of tea extract, 0.5-1.5% of licorice extract, 0.1-1.0% of chamomile flower extract, 0.1-1.0% of rosemary leaf extract, 40-60% of butanediol, and the balance is water.

[0010] Preferably, the preparation process of the verbena extract is as follows:

[0011] (1) taking leaves of verbena and performing disinfection treatment to obtain sterile leaves; inoculating the sterile leaves into a culture medium for induction culture until callus tissue is formed; the culture medium comprises MS culture medium and the following components added to the MS culture medium: sucrose, agar powder, sodium selenite, and γ-oryzanol;

[0012] (2) freeze-drying the callus tissue obtained in step (1) and then crushing it to obtain callus tissue powder; adding the callus tissue powder to an ethanol aqueous solution, heating and refluxing to obtain an extract; and concentrating and freeze-drying the extract to obtain a product.

[0013] Preferably, based on the final concentration of the culture medium, the concentrations of the components in step (1) in the culture medium are: sucrose 10-15 g / L, agar powder 20-40 g / L, sodium selenite 1-5 g / L, and γ-oryzanol 2-8 g / L.

[0014] Preferably, the induction culture conditions in step (1) are: temperature of 20-25°C, light intensity of 200-300lx, and illumination time of 11-13h / d.

[0015] Preferably, in step (2), the solid-liquid ratio of the callus powder to the ethanol aqueous solution is 1:(10-15), and the heating reflux time is 10-15 min.

[0016] Preferably, the concentration of the ethanol aqueous solution in step (2) is 60-75%.

[0017] The second object of the present invention is achieved by adopting the following technical solution:

[0018] The preparation method of the above-mentioned composite plant extract comprises the following steps:

[0019] According to the mass percentage, all the raw materials are mixed evenly.

[0020] The third object of the present invention is achieved by adopting the following technical solution:

[0021] The application of the above-mentioned composite plant extract in the preparation of cosmetics for repairing skin barrier.

[0022] Preferably, based on the total mass of the cosmetic, the mass percentage of the composite plant extract is 1-5%.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] (1) The present invention innovatively adds sodium selenite, γ-oryzanol and other ingredients to the culture medium during the extraction process of verbena extract, wherein sodium selenite can affect the expression of key enzymes or genes in the secondary metabolic pathway of plants, and the antioxidant property of γ-oryzanol helps to maintain the healthy state of callus tissue and promote the normal growth and differentiation of cells. The synergistic effect of the two can effectively increase the total flavonoid content in the verbena extract. The test results of the total flavonoid content show that the total flavonoid content in the verbena extract obtained by the present invention is relatively high, reaching more than 3107ppm.

[0025] (2) The composite plant extract of the present invention has an excellent skin barrier repair effect, and the absolute value of the transepidermal water loss change rate after use is above 46.76%. DETAILED DESCRIPTION

[0026] The present invention will be further described below in conjunction with specific embodiments. It should be noted that, under the premise of no conflict, the embodiments described below or the technical features can be arbitrarily combined to form new embodiments. The specific conditions not specified in the embodiments are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels.

[0027] 1. Embodiment

[0028] Example 1

[0029] Example 1 provides a composite plant extract, which is composed of the following raw materials in percentage by mass: 0.7% verbena extract, 5% Centella asiatica extract, 2% Polygonum cuspidatum root extract, 2% Scutellaria baicalensis root extract, 1% tea extract, 1% Glycyrrhiza glabra extract, 0.5% chamomile flower extract, 0.6% rosemary leaf extract, 52% butylene glycol, and the balance is water.

[0030] Wherein, the preparation process of the verbena extract is as follows:

[0031] (1) The leaves of verbena after washing with water were soaked in 75% alcohol by volume for 30 seconds on a clean bench, then washed with sterile water for 3 times, treated with 0.1% mercuric chloride solution by volume for 5 minutes, and finally washed with sterile water for 6 times, each washing time was 5 minutes, and the surface moisture was absorbed by sterilized filter paper to obtain sterile leaves; the sterile leaves (a small piece of 5 mm wide and 5 mm long) were inoculated into a culture medium, and induced to form callus tissue under the conditions of a temperature of 22° C., a light intensity of 280 lx, and a light time of 12 h / d; the culture medium consisted of MS culture medium and the following components added to the MS culture medium: 12 g / L sucrose, 31 g / L agar powder, 4 g / L sodium selenite, and 6 g / L γ-oryzanol;

[0032] (2) freeze-drying the callus tissue of step (1) and then crushing it to obtain callus tissue powder; adding the callus tissue powder to ethanol aqueous solution (the concentration of ethanol aqueous solution is 70%) according to a solid-liquid ratio of callus tissue powder to ethanol aqueous solution of 1:12, heating and refluxing for 12 minutes to obtain an extract, and concentrating and freeze-drying the extract to obtain the product.

[0033] The preparation process of Centella Asiatica extract is as follows:

[0034] Cut Centella asiatica into sections, use 75% ethanol to reflux and extract twice, the weight ratio of the first feed to liquid is 1:12, and the weight ratio of the second feed to liquid is 1:10, filter each time with three layers of gauze, combine the two filtrates, concentrate the filtrates under reduced pressure and then disperse them in water, extract twice with n-butanol, combine the n-butanol layers, and concentrate under reduced pressure to dryness to obtain the product.

[0035] The preparation process of Polygonum cuspidatum root extract is as follows:

[0036] The root of Polygonum cuspidatum is dried and crushed, and extracted twice with 75% by volume ethanol solution, the weight ratio of the first time being 1:14, and the weight ratio of the second time being 1:9, each time filtered with three layers of gauze, the two filtrates are combined, and the filtrates are concentrated under reduced pressure to obtain the product.

[0037] The preparation process of Scutellaria baicalensis root extract is as follows:

[0038] The root of Scutellaria baicalensis is dried and crushed, and extracted twice with a 75% by volume ethanol solution, the weight ratio of the first time being 1:10, and the weight ratio of the second time being 1:6, and filtered through three layers of gauze each time, and the two filtrates are combined. After the filtrate is concentrated under reduced pressure, the pH value is adjusted to 2.0 with hydrochloric acid, and after standing, the precipitate is filtered out, the supernatant is taken, and the pH value of the supernatant is adjusted to 1.0. After standing and filtering, the obtained precipitate is rinsed with ethanol to a pH value of 7.0, and dried to obtain the product.

[0039] The preparation process of tea extract is as follows:

[0040] According to the weight ratio of material to liquid of 1kg:50L, the tea leaves are soaked in boiling water for 25 minutes, filtered to obtain a filtrate, and the filtrate is concentrated and freeze-dried to obtain the product.

[0041] The preparation process of GLYCYRRHIZA GLABRA extract is as follows:

[0042] According to the weight ratio of material to liquid of 1:8, Glycyrrhiza glabra is crushed, extracted twice with 75% ethanol solution by volume, filtered with three-layer gauze each time, the two filtrates are combined, concentrated under reduced pressure, alcohol is added to the alcohol content of 50%, and the alcohol extract is obtained after filtration; the alcohol extract is eluted with 50% ethanol and 80% ethanol in turn to obtain an eluate, the eluate is concentrated under reduced pressure, extracted with ethyl acetate twice, and treated with petroleum ether sedimentation twice to obtain a supernatant, and the supernatant is concentrated and dried to obtain the product.

[0043] The preparation process of chamomile flower extract and rosemary leaf extract is as follows:

[0044] At a solid-liquid ratio of 1:20, the dry powder of each raw material was added into 90°C pure water, stirred and extracted to obtain an extract. The extract was centrifuged and filtered to obtain a first filtrate and a first filter residue. The first filter residue was then added into 60°C pure water, stirred and extracted, and then centrifuged and filtered to obtain a second filtrate. The two filtrates were combined to obtain the extracts of each raw material.

[0045] Example 1 also provides a method for preparing the above-mentioned composite plant extract, which is as follows:

[0046] According to the above mass percentage, mix the raw materials evenly.

[0047] Example 2

[0048] Example 2 provides a composite plant extract, which is composed of the following raw materials in percentage by weight: 0.5% verbena extract, 4% Centella asiatica extract, 1.5% Polygonum cuspidatum root extract, 1.5% Scutellaria baicalensis root extract, 0.5% tea extract, 0.5% Glycyrrhiza glabra extract, 0.1% Chamomile flower extract, 0.1% Rosemary leaf extract, 40% butylene glycol, and the balance is water.

[0049] Wherein, the preparation process of the verbena extract is as follows:

[0050] (1) The leaves of verbena after washing with water were soaked in 75% alcohol by volume for 30 seconds on a clean bench, then washed with sterile water for 3 times, treated with 0.1% mercuric chloride solution by volume for 5 minutes, and finally washed with sterile water for 6 times, each washing time was 5 minutes, and the surface moisture was absorbed by sterilized filter paper to obtain sterile leaves; the sterile leaves (a small piece of 5 mm wide and 5 mm long) were inoculated into a culture medium, and induced to form callus tissue under the conditions of a temperature of 20° C., a light intensity of 300 lx, and a light time of 13 h / d; the culture medium consisted of MS culture medium and the following components added to the MS culture medium: 10 g / L sucrose, 20 g / L agar powder, 1 g / L sodium selenite, and 2 g / L γ-oryzanol;

[0051] (2) freeze-drying the callus tissue of step (1) and then crushing it to obtain callus tissue powder; adding the callus tissue powder to the ethanol aqueous solution (the concentration of the ethanol aqueous solution is 60%) according to the solid-liquid ratio of the callus tissue powder to the ethanol aqueous solution of 1:10, heating and refluxing for 15 minutes to obtain an extract; and concentrating and freeze-drying the extract to obtain the product.

[0052] The preparation methods of Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, Camellia oleifera extract, Glycyrrhiza glabra extract, Chamomile flower extract and Rosemary leaf extract are the same as those in Example 1.

[0053] Example 2 also provides a method for preparing the above-mentioned composite plant extract, which is as follows:

[0054] According to the above mass percentage, mix the raw materials evenly.

[0055] Example 3

[0056] Example 3 provides a composite plant extract, which is composed of the following raw materials in percentage by mass: 1% verbena extract, 6% Centella asiatica extract, 2.5% Polygonum cuspidatum root extract, 2.5% Scutellaria baicalensis root extract, 1.5% tea extract, 1.5% Glycyrrhiza glabra extract, 1.0% Chamomile flower extract, 1.0% Rosemary leaf extract, 60% butylene glycol, and the balance is water.

[0057] Wherein, the preparation process of the verbena extract is as follows:

[0058] (1) The leaves of verbena after washing with water were soaked in 75% alcohol by volume for 30 seconds on a clean bench, then washed with sterile water for 3 times, treated with 0.1% mercuric chloride solution by volume for 5 minutes, and finally washed with sterile water for 6 times, each washing time was 5 minutes, and the surface moisture was absorbed by sterile filter paper to obtain sterile leaves; the sterile leaves (a small piece of 5 mm wide and 5 mm long) were inoculated into a culture medium, and induced to form callus tissue under the conditions of a temperature of 25°C, a light intensity of 200 lx, and a light time of 11 h / d; the culture medium consisted of MS culture medium and the following components added to the MS culture medium: 15 g / L sucrose, 40 g / L agar powder, 5 g / L sodium selenite, and 8 g / L γ-oryzanol;

[0059] (2) freeze-drying the callus tissue obtained in step (1) and then crushing it to obtain callus tissue powder; adding the callus tissue powder to the ethanol aqueous solution (the concentration of the ethanol aqueous solution is 75%) according to a solid-liquid ratio of the callus tissue powder to the ethanol aqueous solution of 1:15, heating and refluxing for 10 minutes to obtain an extract; and concentrating and freeze-drying the extract to obtain the product.

[0060] The preparation methods of Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, Camellia oleifera extract, Glycyrrhiza glabra extract, Chamomile flower extract and Rosemary leaf extract are the same as those in Example 1.

[0061] Example 3 also provides a method for preparing the above-mentioned composite plant extract, which is as follows:

[0062] According to the above mass percentage, mix the raw materials evenly.

[0063] 2. Comparative Example

[0064] Comparative Example 1

[0065] The difference between Comparative Example 1 and Example 1 is:

[0066] The preparation process of verbena extract is as follows:

[0067] The verbena is dried and crushed to obtain verbena powder, and 30% ethanol is used at a weight ratio of 1:10 at 80°C for extraction for 9 hours to obtain an extract; the extract is subjected to reduced pressure distillation, cooling and suction filtration, and freeze-drying to obtain the extract.

[0068] Comparative Example 2

[0069] The difference between Comparative Example 2 and Example 1 is:

[0070] During the preparation of the verbena extract, the culture medium consists of MS culture medium and the following components added to the MS culture medium: 12 g / L sucrose, 31 g / L agar powder, 6 g / L γ-oryzanol, and the rest is the same as in Example 1.

[0071] Comparative Example 3

[0072] The difference between Comparative Example 3 and Example 1 is that:

[0073] During the preparation of the verbena extract, the culture medium consists of MS culture medium and the following components added to the MS culture medium: 12 g / L sucrose, 31 g / L agar powder, 4 g / L sodium selenite, and the rest is the same as in Example 1.

[0074] Comparative Example 4

[0075] The difference between Comparative Example 4 and Example 1 is that:

[0076] During the preparation of the verbena extract, the culture medium consists of MS culture medium and the following components added to the MS culture medium: 12 g / L sucrose, 31 g / L agar powder, and 10 g / L sodium selenite, and the rest is the same as in Example 1.

[0077] Comparative Example 5

[0078] The difference between Comparative Example 5 and Example 1 is that:

[0079] During the preparation of the verbena extract, the culture medium consists of MS culture medium and the following components added to the MS culture medium: 12 g / L sucrose, 31 g / L agar powder, and 10 g / L γ-oryzanol, and the rest is the same as in Example 1.

[0080] Comparative Example 6

[0081] The difference between Comparative Example 6 and Example 1 is that:

[0082] During the preparation of the verbena extract, the culture medium consists of MS culture medium and the following components added to the MS culture medium: sucrose 12 g / L, agar powder 31 g / L, 2,4-D 1 mg / L, IBA 0.5 mg / L, 6-BA 5 mg / L, and the rest is the same as in Example 1.

[0083] 3. Test examples

[0084] The content of total flavonoids in the verbena extracts obtained in Examples 1-3 and Comparative Examples 1-6 was detected, and the specific process was as follows:

[0085] Weigh 1g of the verbena extract obtained in Examples 1-3 and Comparative Examples 1-6 and dissolve them in 10 times the volume of water to obtain each group of sample solutions. Take 100μL of each group of sample solutions, add 100μL of 5wt% sodium nitrate aqueous solution to each sample, mix well and let stand for 5min, then add 100μL of 10wt% aluminum nitrate aqueous solution, mix well and let stand for 5min, then add 1mL of 4wt% sodium hydroxide aqueous solution and mix well, add 0.7mL of water and mix well to obtain each group of samples to be tested. Take 100μL of each group of samples to be tested, detect the absorbance value at 500nm, use rutin as the reference, and refer to Table 1 for the results of total flavonoid content.

[0086] Table 1

[0087]

[0088]

[0089] It can be seen from Table 1 that the total flavonoids content in the verbena extract obtained by the present invention is relatively high, reaching more than 3107 ppm.

[0090] Compared with Examples 1-3, the total flavonoids content in the verbena extracts obtained in Comparative Examples 1-6 was reduced to varying degrees, among which the total flavonoids content in the verbena extract obtained in Comparative Example 1 was the lowest, which was only 1004 ppm.

[0091] Further analysis shows that the verbena extract obtained in Comparative Example 1 is verbena directly extracted with ethanol; in the process of extracting the verbena extract in Comparative Example 2, sodium selenite was not added to the culture medium; in the process of extracting the verbena extract in Comparative Example 3, γ-oryzanol was not added to the culture medium; in the process of extracting the verbena extract in Comparative Example 4, γ-oryzanol was not added to the culture medium, but the amount of sodium selenite was increased; in the process of extracting the verbena extract in Comparative Example 5, sodium selenite was not added to the culture medium, but the amount of γ-oryzanol was increased. In the process of extracting the verbena extract in Comparative Example 6, sodium selenite and γ-oryzanol were not added to the culture medium. Combined with the results of the total flavonoid content in the verbena extract, it can be seen that the synergistic effect of sodium selenite and γ-oryzanol can effectively increase the total flavonoid content in the verbena extract.

[0092] 4. Application examples

[0093] The effects of adding the composite plant extracts of Examples 1-3 and Comparative Examples 1-6 of the present invention are described below.

[0094] An essence comprises 3% of the above-mentioned composite plant extracts, 0.2% of carbomer, 4% of trehalose, 3% of caprylic and capric triglyceride, and the balance of water.

[0095] Application Example 1

[0096] The safety of the cosmetics obtained by adding the composite plant extracts of Examples 1-3 and Comparative Examples 1-6 was tested, and the specific process is as follows:

[0097] Participants and groups: 90 participants aged 18-45 years were randomly divided into 9 groups, with a male-to-female ratio of 1:1 in each group.

[0098] With reference to the 2015 "Technical Specification for Safety of Cosmetics", the irritation of the essences obtained from the composite plant extracts of Examples 1-3 and Comparative Examples 1-6 was evaluated. The test method was a skin patch test. The test lasted for 24 hours. After removing the test patch tester for 30 minutes, the skin reaction was observed after the indentation disappeared. If the result was negative, the skin reaction was observed again 24 hours and 48 hours after the patch test. The results of the patch test were judged with reference to the skin reaction grading standards in Table 2. The results are shown in Table 3.

[0099] Table 2

[0100]

[0101]

[0102] Table 3

[0103] Group 30min 24h 48h Cosmetics containing the extract of Example 1 All 10 people are level 0 All 10 people are level 0 All 10 people are level 0 Cosmetics containing the extract of Example 2 All 10 people are level 0 All 10 people are level 0 All 10 people are level 0 Cosmetics containing the extract of Example 3 All 10 people are level 0 All 10 people are level 0 All 10 people are level 0 Cosmetics containing the extract of Comparative Example 1 All 10 people are level 0 All 10 people are level 0 All 10 people are level 0 Cosmetics containing the extract of Comparative Example 2 All 10 people are level 0 All 10 people are level 0 All 10 people are level 0 Cosmetics containing the extract of Comparative Example 3 All 10 people are level 0 All 10 people are level 0 All 10 people are level 0 Cosmetics containing the extract of Comparative Example 4 All 10 people are level 0 All 10 people are level 0 All 10 people are level 0 Cosmetics containing the extract of Comparative Example 5 All 10 people are level 0 All 10 people are level 0 All 10 people are level 0 Cosmetics containing the extract of Comparative Example 6 All 10 people are level 0 All 10 people are level 0 All 10 people are level 0

[0104] As can be seen from Table 3, the extract of the present invention has no irritation to the skin and is safe to use.

[0105] Application Example 2

[0106] The skin repair barrier ability of the cosmetics obtained by adding the composite plant extracts of Examples 1-3 and Comparative Examples 1-6 was tested, and the specific process is as follows:

[0107] Participants and groups: 90 participants aged 18-45 years were randomly divided into 9 groups, with a male-to-female ratio of 1:1 in each group.

[0108] Test environment: temperature 20℃, humidity 54%, avoid direct sunlight.

[0109] Test method: All subjects began the test 30 minutes after entering the test environment. The subjects applied the essence added with the composite plant extracts of Examples 1-3 and Comparative Examples 1-6 on their faces once in the morning and evening every day, and the skin water evaporation (TEWL value) was tested before use and 28 days after using the skin care products. Each group of data is the average value of the test results of the group.

[0110] Among them, the change rate of transepidermal water loss (%) = (TEWL value after using the cosmetics for 28 days - TEWL value before use) / TEWL value before use × 100%, see Table 4 for the results.

[0111] Table 4

[0112]

[0113]

[0114] The higher the absolute value of the transepidermal water loss change rate data, the better the repair effect of the cosmetics and the better the skin barrier formation. It can be seen from Table 4 that the cosmetics of the present invention have excellent skin repair barrier effects. Specifically, after using the cosmetics of the present invention for 28 days, the absolute value of the transepidermal water loss change rate is above 46.76%. Compared with Examples 1-3, the skin repair barrier effects of the cosmetics in Comparative Examples 1-6 are reduced to varying degrees, and Comparative Examples 1-6 all adjusted the preparation process of the verbena extract. Combined with the results of the total flavonoid content of the verbena extract, it is known that the increase in the total flavonoid content in the verbena extract can enhance the skin repair barrier effect of the cosmetics.

[0115] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A composite plant extract, characterized in that: The invention comprises the following raw materials in percentage by weight: 0.5-1% of verbena extract, 4-6% of centella asiatica extract, 1.5-2.5% of polygonum cuspidatum root extract, 1.5-2.5% of scutellaria baicalensis root extract, 0.5-1.5% of tea extract, 0.5-1.5% of licorice extract, 0.1-1.0% of chamomile flower extract, 0.1-1.0% of rosemary leaf extract, 40-60% of butylene glycol and the balance is water.

2. The composite plant extract according to claim 1, characterized in that The preparation process of the verbena extract is as follows: (1) taking leaves of verbena and performing disinfection treatment to obtain sterile leaves; inoculating the sterile leaves into a culture medium for induction culture until callus tissue is formed; the culture medium comprises MS culture medium and the following components added to the MS culture medium: sucrose, agar powder, sodium selenite, and γ-oryzanol; (2) freeze-drying the callus tissue obtained in step (1) and then crushing it to obtain callus tissue powder; adding the callus tissue powder to an ethanol aqueous solution, heating and refluxing to obtain an extract; and concentrating and freeze-drying the extract to obtain a product.

3. The composite plant extract according to claim 2, characterized in that: Based on the final concentration of the culture medium, the concentrations of the components in step (1) in the culture medium are: sucrose 10-15 g / L, agar powder 20-40 g / L, sodium selenite 1-5 g / L, and γ-oryzanol 2-8 g / L.

4. The composite plant extract according to claim 2, characterized in that: The induction culture conditions in step (1) are: temperature of 20-25°C, light intensity of 200-300lx, and light duration of 11-13h / d.

5. The composite plant extract according to claim 2, characterized in that: In step (2), the solid-liquid ratio of the callus powder to the ethanol aqueous solution is 1:(10-15), and the heating reflux time is 10-15 minutes.

6. The composite plant extract according to claim 2, characterized in that: The concentration of the ethanol aqueous solution in step (2) is 60-75%.

7. The method for preparing the composite plant extract according to claim 1, characterized in that: The following steps are involved: According to the mass percentage, all the raw materials are mixed evenly.

8. The use of the composite plant extract according to claim 1, characterized in that: Application in the preparation of cosmetics for repairing skin barrier.

9. The use of the composite plant extract according to claim 8, characterized in that: Based on the total mass of the cosmetics, the mass percentage of the composite plant extract is 1-5%.

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