Natural plant composition with sunscreen effect and preparation method thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a natural plant composition with a sunscreen effect and a preparation method thereof. According to the sunscreen composition, active substances in rosa roxburghii tratt fruits, zanthoxylum bungeanum fruits and oriental cherry flowers are extracted by adopting a special preparation process respectively, and then the extracts are compounded and are matched with one or more of ethylhexyl methoxycinnamate, octocrylene, avobenzone and ethylhexyl triazinone for use, so that the problems that a chemical sunscreen agent is poor in light stability, the sunscreen effect is poor and the sunscreen effect is poor are solved. According to the present invention, the problems of skin sensitivity and phototoxicity can be caused due to the influence of the components in the composition, the effects of the components can be completely performed, and the sunscreen agent has characteristics of good sunscreen effect, skin whitening and nourishing effect, and safety. In addition, the composition can reduce percutaneous penetration of the sun-screening agent to a certain extent, and further reduce side effects of the sun-screening agent on the skin, so that the effect of protecting the skin is achieved, and the composition is safe and reliable. The plant raw materials are high in availability and low in production cost.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a natural plant composition with sunscreen effect and a preparation method thereof. Background Art

[0002] With environmental pollution and the destruction of the global atmosphere, more ultraviolet rays, high-energy blue light and infrared rays are irradiating the earth's surface. Various harmful lights have caused certain damage to the human body, and people are paying more and more attention to sun protection.

[0003] In order to reduce the damage of ultraviolet rays to the skin, sunscreen products are usually used to protect the skin. At present, sunscreens are divided into three categories: physical sunscreens, chemical sunscreens, and biological sunscreens. Physical sunscreens scatter and reflect ultraviolet rays by adding inorganic ions such as nano-grade titanium dioxide or ZnO, reducing the contact of ultraviolet rays with the skin, thereby preventing damage to the skin. This type of sunscreen is chemically inert and helps to stabilize the product when added to cosmetics. Physical sunscreens with high sun protection index do not feel good on the skin. Chemical sunscreens mainly protect the skin from ultraviolet damage by absorbing ultraviolet rays and converting ultraviolet energy into heat energy or harmless visible light effects. According to the different absorption bands, they can be divided into UVA absorbers and UVB absorbers. Chemical sunscreen products are light and non-sticky, but have poor photostability, which can cause skin sensitivity and phototoxicity, as well as environmental problems. These physical and chemical sunscreen additives are easy to clog pores and even breed acne and blackheads. Biological sunscreens are mainly some natural animal and plant extracts. They achieve sun protection through ultraviolet absorption. They are non-irritating and have no toxic side effects. They can also scavenge free radicals and have a good effect on repairing skin after sun exposure.

[0004] The main ingredients in roxburghii fruit that have sunscreen and skin care effects include vitamin C, superoxide dismutase (SOD), roxburghii flavonoids, roxburghii polysaccharides, and a variety of essential amino acids and trace elements for the human body. These ingredients work together to give roxburghii fruit powerful antioxidant, moisturizing, antibacterial, anti-inflammatory and sunscreen capabilities. Roxburghii fruit extracts are widely used in cosmetics, and some sunscreen products contain roxburghii fruit ingredients that can absorb or reflect ultraviolet rays and protect the skin from ultraviolet damage.

[0005] The main sunscreen skincare ingredient in Sichuan pepper fruit is sanshool. Sanshool is an active ingredient extracted from Sichuan pepper that has powerful antioxidant and skin repair capabilities. It can resist the invasion of ultraviolet rays, scavenge reactive oxygen free radicals, and protect the skin from photodamage. In addition, sanshool can reduce skin photosensitivity, inhibit inflammation, promote the growth of dermal fibroblasts, improve wrinkles, and has soothing and calming effects. Sanshool is widely used in skincare products and is often used in care products for sensitive skin. It can reduce skin damage caused by ultraviolet rays, promote the growth of skin cells, enhance skin vitality, and improve skin condition.

[0006] Cherry blossoms are woody flowering plants of the genus Prunus in the Rosaceae family, and are cultivated all over the world. The kernels and leaves of cherry blossoms can be used as medicine, and the flowers of cherry blossoms have edible value and can be used as jams, desserts, condiments, etc. The petals can be dried and made into tea, which has the effect of rejuvenating and whitening the skin and is often used as a raw material for cosmetics. Cherry blossoms contain sakura glycosides and sakura glycosides, which have been gradually used in skin care products and cosmetics in recent years to help improve skin condition. In addition, sakura glycosides also have antiviral, anti-cancer, anti-inflammatory, and anti-allergic properties, and have great application prospects in the fields of health care products, medicines, and skin care products.

[0007] Using natural extracts as sunscreen is a relatively safe and environmentally friendly method nowadays, but it is difficult for a single natural product to achieve a high sun protection index. In addition, how to prevent sunscreen from penetrating through the skin into the systemic circulation and causing adverse reactions is also an important factor to be considered when developing sunscreen products. Nowadays, most of them are modified by sunscreen (CN109771306A) or silicone resin (CN101316577B), dextran (CN107997976A), marine bioactive substances (CN102302417B), etc. are added to the formula to reduce the percutaneous penetration of sunscreen, which not only has a complex process and a high formula cost, but is also not conducive to production and operation.

[0008] Based on this, we propose a natural plant composition with sunscreen effect and a preparation method thereof, hoping to solve the deficiencies in the prior art. Summary of the invention

[0009] The purpose of the present invention is to provide a natural plant composition with sunscreen effect and a preparation method thereof in view of the existing problems.

[0010] The present invention is achieved through the following technical solutions:

[0011] A natural plant composition with sunscreen effect, comprising the following raw materials in parts by weight:

[0012] 2-4 parts of chemical sunscreen, 20-32 parts of plant extract, 30-40 parts of oil, 2-4 parts of emulsifier, 3-6 parts of moisturizer, 40-60 parts of ultrapure water.

[0013] More preferably, the chemical sunscreen is one or more of ethylhexyl methoxycinnamate, octocrylene, avobenzone, and ethylhexyl triazone.

[0014] Further preferably, the preparation of the plant extract comprises the following steps:

[0015] (1) placing fresh roxburghii fruit in a microfluidizer for high-pressure homogenization, soaking and extracting the obtained homogenized product with 70% ethanol for 3 to 4 times, filtering, combining the extracts and collecting roxburghii pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a roxburghii fruit extract;

[0016] (2) placing fresh Zanthoxylum bungeanum fruit in a microfluidizer for high-pressure homogenization, soaking and extracting the obtained homogenized product with 70% ethanol for 3 to 4 times, filtering, combining the extracts and collecting the Zanthoxylum bungeanum pomace, and concentrating the combined extracts by reduced-pressure evaporation to obtain a Zanthoxylum bungeanum extract;

[0017] (3) After the freshly picked cherry blossoms are washed with clean water, they are placed in a freeze drying box for vacuum freeze drying, and after drying, they are placed in a deep cold mill for pulverization, and then extracted with anhydrous ethanol as an extraction solvent under the assistance of ultrasound, the original solution and cherry blossom residue are collected, and the extracted original solution is concentrated by reduced pressure evaporation at 60° C. to obtain cherry blossom extract after concentration;

[0018] (4) The prickly ash pear residue obtained in step (1), the prickly ash pomace obtained in step (2), and the cherry pomace obtained in step (3) are placed in an oven and dried to constant weight, respectively, and then added to a grinder in a ratio of 3-4:2-3:1 for grinding, passed through an 80-mesh sieve, added to a NaOH solution, hydrolyzed in a water bath at 80-90° C. for 1-2 h, neutralized with hydrochloric acid, centrifuged at 10000-12000 rpm for 10-15 min, washed the precipitate with deionized water for 3-5 times, added to a glacial acetic acid solution, stirred at 200-300 rpm for 1-2 h in a water bath at 80-90° C., centrifuged at 10000-12000 rpm for 10-15 min, washed the precipitate with deionized water, and finally dried to obtain cellulose.

[0019] (5) weighing 8 to 12 parts of the roxburghii fruit extract, 2 to 5 parts of the zanthoxylum fruit extract, 6 to 10 parts of the cherry blossom extract, and 0.6 to 0.8 parts of cellulose in corresponding weight portions for later use;

[0020] (6) The roxburghii fruit extract, Zanthoxylum bungeanum fruit extract, cherry blossom extract, and cellulose weighed above are sequentially added into a homogenizer for homogenization to obtain a plant extract.

[0021] Further preferably, the pressure of the high-pressure homogenization treatment in step (1) is 100-120 MPa, and the treatment time is 2-3 min;

[0022] The pressure of the high-pressure homogenization treatment in step (2) is 100-120 MPa, and the treatment time is 1-2 min;

[0023] Further preferably, during the vacuum freeze drying in step (3), the vacuum degree is controlled to be 15 to 20 kPa, the cold trap temperature is -60 to -40°C, the holding time is 30 to 60 min, and the drying temperature is 3 to 15°C;

[0024] During deep cold crushing, the temperature is controlled at -80 to -40°C and the crushing process is 2 to 3 minutes.

[0025] Further preferably, the concentration of the NaOH solution in step (4) is 17-21%;

[0026] The concentration of hydrochloric acid is 35-39%;

[0027] The pH value of glacial acetic acid solution is 3-4.

[0028] Further preferably, the oil is one or more of wheat germ oil, coconut oil, camellia oil, and hazelnut oil;

[0029] The emulsifier is one or more of lecithin, epoxidized castor oil glyceride, and polyoxyethylene lanolin;

[0030] The moisturizing agent is one or more of trehalose, butylene glycol, propylene glycol and betaine;

[0031] A method for preparing a natural plant composition with sunscreen effect comprises the following steps:

[0032] S1, adding the plant extract, moisturizer and ultrapure water into a stirring tank, stirring and mixing to obtain a first mixed solution;

[0033] S2, heating and mixing the chemical sunscreen and the oil to obtain a second mixed solution;

[0034] S3, adding the emulsifier to the second mixed solution, homogenizing it, adding the first mixed solution, and homogenizing it again.

[0035] More preferably, the temperature of the heating and mixing in step S2 is 80-90°C.

[0036] Compared with the prior art, the present invention has the following advantages:

[0037] The present invention adopts a special preparation process to extract the active substances in roxburghii fruit, prickly ash fruit and cherry blossom respectively, and the extraction of active substances is improved to the greatest extent, and then several extracts are compounded and used to obtain a sunscreen with a higher sun protection index, and plant extracts are used in conjunction with chemical sunscreens, moisturizers, emulsifiers, etc., and under mutual influence, the effect of each component is fully exerted, and the sunscreen not only has a good sunscreen effect, but also has the effect of whitening and nourishing the skin, and has safety. In addition, the present invention makes the extraction residue obtained in the preparation process of roxburghii fruit, prickly ash fruit and cherry blossom extract into cellulose, which is added to the composition according to a suitable ratio, and can be used as a thickener, and the use of roxburghii fruit, prickly ash fruit and cherry blossom extract can effectively reduce the transdermal penetration of sunscreen, thereby further reducing the side effect of sunscreen to skin, thereby playing the role of protecting skin, safe and reliable. And the raw material is taken from the extraction residue, which more effectively improves the utilization of plant raw materials, and reduces production costs to a great extent. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a comparison chart of the DPPH free radical scavenging ability of each sample of the present invention;

[0039] Figure 2 It is a comparative example of the ABTS cation radical scavenging ability of each sample of the present invention. DETAILED DESCRIPTION

[0040] In order to further explain the present invention, it is described below in conjunction with the following specific embodiments.

[0041] Example 1

[0042] A natural plant composition with sunscreen effect, comprising the following raw materials in parts by weight:

[0043] 2 parts of ethylhexyl methoxycinnamate, 20 parts of plant extract, 30 parts of wheat germ oil, 2 parts of lecithin, 3 parts of trehalose, and 40 parts of ultrapure water.

[0044] The preparation of the plant extract comprises the following steps:

[0045] (1) placing fresh roxburghii fruit in a microfluidizer at 100 MPa for 2 min, soaking and extracting the obtained homogenized product with 70% ethanol for 3 times, filtering, combining the extracts and collecting the roxburghii pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a roxburghii fruit extract;

[0046] (2) placing fresh Zanthoxylum bungeanum fruit in a microfluidizer at 100 MPa for 1 min, soaking and extracting the obtained homogenized product with 70% ethanol for 3 times, filtering, combining the extracts and collecting the Zanthoxylum bungeanum pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a Zanthoxylum bungeanum extract;

[0047] (3) After the freshly picked cherry blossoms are cleaned with clean water, they are placed in a freeze drying box for vacuum freeze drying, the vacuum degree is controlled to be 15 kPa, the cold trap temperature is -60°C, the holding time is 30 min, and the drying temperature is 3°C. After drying, they are placed in a deep cold mill for pulverization, the temperature is controlled to be -80°C, and the pulverization process is performed for 2 min. Then, under the assistance of ultrasound, anhydrous ethanol is used as the extraction solvent for extraction, the stock solution and cherry blossom residue are collected, and the extracted stock solution is evaporated and concentrated under reduced pressure at 60°C to obtain cherry blossom extract after concentration;

[0048] (4) The prickly ash pomace obtained in step (1), the prickly ash pomace obtained in step (2), and the cherry pomace obtained in step (3) are placed in an oven and dried to constant weight, respectively, and then added to a grinder in a ratio of 3:2:1 for grinding, passed through an 80-mesh sieve, added to a NaOH solution (17%), hydrolyzed in a water bath at 80° C. for 1 hour, neutralized with hydrochloric acid (35%), centrifuged at 10,000 rpm for 10 minutes, washed the precipitate with deionized water for three times, added to a glacial acetic acid solution with a pH value of 3, stirred at 200 rpm for 1 hour in a water bath at 80° C., centrifuged at 10,000 rpm for 10 minutes, washed the precipitate with deionized water, and finally dried to obtain cellulose.

[0049] (5) Weigh 8 parts of the roxburghii fruit extract, 2 parts of the zanthoxylum fruit extract, 6 parts of the cherry blossom extract, and 0.6 parts of cellulose in corresponding weight portions for later use;

[0050] (6) The roxburghii fruit extract, Zanthoxylum bungeanum fruit extract, cherry blossom extract, and cellulose weighed above are sequentially added into a homogenizer for homogenization to obtain a plant extract.

[0051] A method for preparing a natural plant composition with sunscreen effect comprises the following steps:

[0052] S1, adding plant extract, trehalose and ultrapure water into a stirring tank, stirring and mixing to obtain a first mixed solution;

[0053] S2, heating ethylhexyl methoxycinnamate and wheat germ oil to 80° C. to obtain a second mixed solution;

[0054] S3, adding lecithin to the second mixed solution, after homogenization, adding the first mixed solution, and homogenizing again.

[0055] Example 2

[0056] A natural plant composition with sunscreen effect, comprising the following raw materials in parts by weight:

[0057] 3 parts of ethylhexyl methoxycinnamate, 26 parts of plant extract, 35 parts of wheat germ oil, 3 parts of lecithin, 4.5 parts of trehalose, and 50 parts of ultrapure water.

[0058] The preparation of the plant extract comprises the following steps:

[0059] (1) placing fresh roxburghii fruit in a microfluidizer at 110 MPa for 2.5 min, soaking and extracting the obtained homogenized product with 70% ethanol for 3.5 times, filtering, combining the extracts and collecting roxburghii pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a roxburghii fruit extract;

[0060] (2) placing fresh Zanthoxylum bungeanum fruit in a microfluidizer at 110 MPa for 1.5 min, soaking and extracting the obtained homogenized product with 70% ethanol for 3 times, filtering, combining the extracts and collecting the Zanthoxylum bungeanum pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a Zanthoxylum bungeanum extract;

[0061] (3) After the freshly picked cherry blossoms are washed with clean water, they are placed in a freeze drying box for vacuum freeze drying, the vacuum degree is controlled to be 17 kPa, the cold trap temperature is -50°C, the holding time is 45 min, and the drying temperature is 9°C. After drying, they are placed in a deep cold mill for pulverization, the temperature is controlled to be -60°C, and the pulverization process is performed for 2.5 min. Then, under the assistance of ultrasound, anhydrous ethanol is used as the extraction solvent for extraction, the stock solution and cherry blossom residue are collected, and the extracted stock solution is evaporated and concentrated under reduced pressure at 60°C to obtain cherry blossom extract after concentration;

[0062] (4) The prickly ash pear residue obtained in step (1), the prickly ash pomace obtained in step (2), and the cherry pomace obtained in step (3) are placed in an oven and dried to constant weight, respectively, and then added to a grinder in a ratio of 3.5:2.5:1 for grinding, passed through an 80-mesh sieve, added to a NaOH solution (19%), hydrolyzed in a water bath at 85° C. for 1.5 h, neutralized with hydrochloric acid (37%), centrifuged at 11000 rpm for 13 min, washed the precipitate with deionized water for 4 times, added to a glacial acetic acid solution with a pH value of 3.5, stirred at 250 rpm for 1.5 h in a water bath at 85° C., centrifuged at 11000 rpm for 13 min, washed the precipitate with deionized water, and finally dried to obtain cellulose.

[0063] (5) Weigh 10 parts of the roxburghii fruit extract, 3 parts of the zanthoxylum fruit extract, 8 parts of the cherry blossom extract, and 0.7 parts of cellulose in corresponding weight portions for later use;

[0064] (6) The roxburghii fruit extract, Zanthoxylum bungeanum fruit extract, cherry blossom extract, and cellulose weighed above are sequentially added into a homogenizer for homogenization to obtain a plant extract.

[0065] A method for preparing a natural plant composition with sunscreen effect comprises the following steps:

[0066] S1, adding plant extract, trehalose and ultrapure water into a stirring tank, stirring and mixing to obtain a first mixed solution;

[0067] S2, heating ethylhexyl methoxycinnamate and wheat germ oil at 85° C. to obtain a second mixed solution;

[0068] S3, adding lecithin to the second mixed solution, after homogenization, adding the first mixed solution, and homogenizing again.

[0069] Example 3

[0070] A natural plant composition with sunscreen effect, comprising the following raw materials in parts by weight:

[0071] 4 parts of ethylhexyl methoxycinnamate, 32 parts of plant extract, 40 parts of wheat germ oil, 4 parts of lecithin, 6 parts of trehalose, and 60 parts of ultrapure water.

[0072] The preparation of the plant extract comprises the following steps:

[0073] (1) placing fresh roxburghii fruit in a microfluidizer at 120 MPa for 3 min, soaking and extracting the obtained homogenized product with 70% ethanol for 4 times, filtering, combining the extracts and collecting the roxburghii pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a roxburghii fruit extract;

[0074] (2) placing fresh Zanthoxylum bungeanum fruit in a microfluidizer at 120 MPa for 2 min, soaking and extracting the obtained homogenized product with 70% ethanol for 4 times, filtering, combining the extracts and collecting the Zanthoxylum bungeanum pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a Zanthoxylum bungeanum extract;

[0075] (3) After the freshly picked cherry blossoms are cleaned with clean water, they are placed in a freeze drying box for vacuum freeze drying, the vacuum degree is controlled to be 20 kPa, the cold trap temperature is -40 ° C, the holding time is 60 min, and the drying temperature is 15 ° C. After drying, they are placed in a deep cold mill for pulverization, the temperature is controlled to be -40 ° C, and the pulverization process is carried out for 3 minutes. Then, under the assistance of ultrasound, anhydrous ethanol is used as the extraction solvent for extraction, the original solution and cherry blossom residue are collected, and the extracted original solution is evaporated and concentrated under reduced pressure at 60 ° C to obtain cherry blossom extract after concentration;

[0076] (4) The prickly ash pear residue obtained in step (1), the prickly ash pomace obtained in step (2), and the cherry pomace obtained in step (3) are placed in an oven and dried to constant weight, respectively, and then added to a grinder in a ratio of 4:3:1 for grinding, passed through an 80-mesh sieve, added to a NaOH solution (21%), hydrolyzed in a 90° C. water bath for 2 h, neutralized with hydrochloric acid (39%), centrifuged at 12,000 rpm for 15 min, washed the precipitate with deionized water for 5 times, added to a glacial acetic acid solution with a pH value of 4, stirred at 300 rpm for 2 h in a 90° C. water bath, centrifuged at 12,000 rpm for 15 min, washed the precipitate with deionized water, and finally dried to obtain cellulose.

[0077] (5) Weigh 12 parts of the roxburghii fruit extract, 5 parts of the zanthoxylum fruit extract, 10 parts of the cherry blossom extract, and 0.8 parts of cellulose in corresponding weight portions for later use;

[0078] (6) The roxburghii fruit extract, Zanthoxylum bungeanum fruit extract, cherry blossom extract, and cellulose weighed above are sequentially added into a homogenizer for homogenization to obtain a plant extract.

[0079] A method for preparing a natural plant composition with sunscreen effect comprises the following steps:

[0080] S1, adding plant extract, trehalose and ultrapure water into a stirring tank, stirring and mixing to obtain a first mixed solution;

[0081] S2, heating ethylhexyl methoxycinnamate and wheat germ oil at 90° C. to obtain a second mixed solution;

[0082] S3, adding lecithin to the second mixed solution, after homogenization, adding the first mixed solution, and homogenizing again.

[0083] Comparative Example 1

[0084] A natural plant composition with sunscreen effect, comprising the following raw materials in parts by weight:

[0085] 26 parts of plant extract, 35 parts of wheat germ oil, 3 parts of lecithin, 4.5 parts of trehalose, and 50 parts of ultrapure water.

[0086] The preparation of the plant extract comprises the following steps:

[0087] (1) placing fresh roxburghii fruit in a microfluidizer at 110 MPa for 2.5 min, soaking and extracting the obtained homogenized product with 70% ethanol for 3.5 times, filtering, combining the extracts and collecting roxburghii pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a roxburghii fruit extract;

[0088] (2) placing fresh Zanthoxylum bungeanum fruit in a microfluidizer at 110 MPa for 1.5 min, soaking and extracting the obtained homogenized product with 70% ethanol for 3 times, filtering, combining the extracts and collecting the Zanthoxylum bungeanum pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a Zanthoxylum bungeanum extract;

[0089] (3) After the freshly picked cherry blossoms are washed with clean water, they are placed in a freeze drying box for vacuum freeze drying, the vacuum degree is controlled to be 17 kPa, the cold trap temperature is -50°C, the holding time is 45 min, and the drying temperature is 9°C. After drying, they are placed in a deep cold mill for pulverization, the temperature is controlled to be -60°C, and the pulverization process is performed for 2.5 min. Then, under the assistance of ultrasound, anhydrous ethanol is used as the extraction solvent for extraction, the stock solution and cherry blossom residue are collected, and the extracted stock solution is evaporated and concentrated under reduced pressure at 60°C to obtain cherry blossom extract after concentration;

[0090] (4) The prickly ash pear residue obtained in step (1), the prickly ash pomace obtained in step (2), and the cherry pomace obtained in step (3) are placed in an oven and dried to constant weight, respectively, and then added to a grinder in a ratio of 3.5:2.5:1 for grinding, passed through an 80-mesh sieve, added to a NaOH solution (19%), hydrolyzed in a water bath at 85° C. for 1.5 h, neutralized with hydrochloric acid (37%), centrifuged at 11000 rpm for 13 min, washed the precipitate with deionized water for 4 times, added to a glacial acetic acid solution with a pH value of 3.5, stirred at 250 rpm for 1.5 h in a water bath at 85° C., centrifuged at 11000 rpm for 13 min, washed the precipitate with deionized water, and finally dried to obtain cellulose.

[0091] (5) Weigh 10 parts of the roxburghii fruit extract, 3 parts of the zanthoxylum fruit extract, 8 parts of the cherry blossom extract, and 0.7 parts of cellulose in corresponding weight portions for later use;

[0092] (6) The roxburghii fruit extract, Zanthoxylum bungeanum fruit extract, cherry blossom extract, and cellulose weighed above are sequentially added into a homogenizer for homogenization to obtain a plant extract.

[0093] A method for preparing a natural plant composition with sunscreen effect comprises the following steps:

[0094] S1, adding plant extract, trehalose and ultrapure water into a stirring tank, stirring and mixing to obtain a first mixed solution;

[0095] S2, heating the wheat germ oil to 85° C. to obtain a second mixed solution;

[0096] S3, adding lecithin to the second mixed solution, after homogenization, adding the first mixed solution, and homogenizing again.

[0097] Comparative Example 2

[0098] A natural plant composition with sunscreen effect, comprising the following raw materials in parts by weight:

[0099] 3 parts of ethylhexyl methoxycinnamate, 26 parts of plant extract, 35 parts of wheat germ oil, 3 parts of lecithin, 4.5 parts of trehalose, and 50 parts of ultrapure water.

[0100] The preparation of the plant extract comprises the following steps:

[0101] (1) placing fresh Zanthoxylum bungeanum fruit in a microfluidizer at 110 MPa for 1.5 min, soaking and extracting the obtained homogenized product with 70% ethanol for 3 times, filtering, combining the extracts and collecting the Zanthoxylum bungeanum pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a Zanthoxylum bungeanum extract;

[0102] (2) After the freshly picked cherry blossoms are washed with clean water, they are placed in a freeze drying box for vacuum freeze drying, the vacuum degree is controlled to be 17 kPa, the cold trap temperature is -50°C, the holding time is 45 min, and the drying temperature is 9°C. After drying, they are placed in a deep cold mill for pulverization, the temperature is controlled to be -60°C, and the pulverization process is performed for 2.5 min. Then, under the assistance of ultrasound, anhydrous ethanol is used as the extraction solvent for extraction, the stock solution and cherry blossom residue are collected, and the extracted stock solution is concentrated by reduced pressure evaporation at 60°C to obtain cherry blossom extract after concentration;

[0103] (3) The prickly ash pomace obtained in step (1) and the cherry pomace obtained in step (2) are placed in an oven and dried to constant weight, respectively, and then added to a grinder in a ratio of 3.5:2.5:1 for grinding, passed through an 80-mesh sieve, added to a NaOH solution (19%), hydrolyzed in a water bath at 85° C. for 1.5 h, neutralized with hydrochloric acid (37%), centrifuged at 11000 rpm for 13 min, washed the precipitate with deionized water for 4 times, added to a glacial acetic acid solution with a pH value of 3.5, stirred at 250 rpm for 1.5 h in a water bath at 85° C., centrifuged at 11000 rpm for 13 min, washed the precipitate with deionized water, and finally dried to obtain cellulose.

[0104] (5) Weigh 3 parts of Zanthoxylum bungeanum fruit extract, 8 parts of cherry blossom extract, and 0.7 parts of cellulose in corresponding weight portions for later use;

[0105] (6) The Zanthoxylum bungeanum fruit extract, cherry blossom extract, and cellulose weighed above are sequentially added into a homogenizer for homogenization to obtain a plant extract.

[0106] A method for preparing a natural plant composition with sunscreen effect comprises the following steps:

[0107] S1, adding plant extract, trehalose and ultrapure water into a stirring tank, stirring and mixing to obtain a first mixed solution;

[0108] S2, heating ethylhexyl methoxycinnamate and wheat germ oil at 85° C. to obtain a second mixed solution;

[0109] S3, adding lecithin to the second mixed solution, after homogenization, adding the first mixed solution, and homogenizing again.

[0110] Comparative Example 3

[0111] A natural plant composition with sunscreen effect, comprising the following raw materials in parts by weight:

[0112] 3 parts of ethylhexyl methoxycinnamate, 26 parts of plant extract, 35 parts of wheat germ oil, 3 parts of lecithin, 4.5 parts of trehalose, and 50 parts of ultrapure water.

[0113] The preparation of the plant extract comprises the following steps:

[0114] (1) placing fresh roxburghii fruit in a microfluidizer at 110 MPa for 2.5 min, soaking and extracting the obtained homogenized product with 70% ethanol for 3.5 times, filtering, combining the extracts and collecting roxburghii pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a roxburghii fruit extract;

[0115] (2) After the freshly picked cherry blossoms are washed with clean water, they are placed in a freeze drying box for vacuum freeze drying, the vacuum degree is controlled to be 17 kPa, the cold trap temperature is -50°C, the holding time is 45 min, and the drying temperature is 9°C. After drying, they are placed in a deep cold mill for pulverization, the temperature is controlled to be -60°C, and the pulverization process is performed for 2.5 min. Then, under the assistance of ultrasound, anhydrous ethanol is used as the extraction solvent for extraction, the stock solution and cherry blossom residue are collected, and the extracted stock solution is concentrated by reduced pressure evaporation at 60°C to obtain cherry blossom extract after concentration;

[0116] (3) The roxburghii pear residue obtained in step (1) and the cherry residue obtained in step (2) are placed in an oven and dried to constant weight, respectively, and then added to a grinder in a ratio of 3.5:2.5:1 for grinding, passed through an 80-mesh sieve, added to a NaOH solution (19%), hydrolyzed in a water bath at 85° C. for 1.5 h, neutralized with hydrochloric acid (37%), centrifuged at 11000 rpm for 13 min, washed the precipitate with deionized water for 4 times, added to a glacial acetic acid solution with a pH value of 3.5, stirred at 250 rpm for 1.5 h in a water bath at 85° C., centrifuged at 11000 rpm for 13 min, washed the precipitate with deionized water, and finally dried to obtain cellulose.

[0117] (5) Weigh 10 parts of the roxburghii fruit extract, 8 parts of the cherry blossom extract, and 0.7 parts of cellulose in corresponding weight portions for later use;

[0118] (6) The roxburghii fruit extract, cherry blossom extract, and cellulose weighed above are sequentially added into a homogenizer for homogenization to obtain a plant extract.

[0119] A method for preparing a natural plant composition with sunscreen effect comprises the following steps:

[0120] S1, adding plant extract, trehalose and ultrapure water into a stirring tank, stirring and mixing to obtain a first mixed solution;

[0121] S2, heating ethylhexyl methoxycinnamate and wheat germ oil at 85° C. to obtain a second mixed solution;

[0122] S3, adding lecithin to the second mixed solution, after homogenization, adding the first mixed solution, and homogenizing again.

[0123] Comparative Example 4

[0124] A natural plant composition with sunscreen effect, comprising the following raw materials in parts by weight:

[0125] 3 parts of ethylhexyl methoxycinnamate, 26 parts of plant extract, 35 parts of wheat germ oil, 3 parts of lecithin, 4.5 parts of trehalose, and 50 parts of ultrapure water.

[0126] The preparation of the plant extract comprises the following steps:

[0127] (1) placing fresh roxburghii fruit in a microfluidizer at 110 MPa for 2.5 min, soaking and extracting the obtained homogenized product with 70% ethanol for 3.5 times, filtering, combining the extracts and collecting roxburghii pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a roxburghii fruit extract;

[0128] (2) placing fresh Zanthoxylum bungeanum fruit in a microfluidizer at 110 MPa for 1.5 min, soaking and extracting the obtained homogenized product with 70% ethanol for 3 times, filtering, combining the extracts and collecting the Zanthoxylum bungeanum pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a Zanthoxylum bungeanum extract;

[0129] (3) The prickly ash pear residue obtained in step (1) and the prickly ash pear residue obtained in step (2) are placed in an oven and dried to constant weight, respectively, and then added to a grinder in a ratio of 3.5:2.5:1 for grinding, passed through an 80-mesh sieve, added to a NaOH solution (19%), hydrolyzed in a water bath at 85° C. for 1.5 h, neutralized with hydrochloric acid (37%), centrifuged at 11000 rpm for 13 min, washed the precipitate with deionized water for 4 times, added to a glacial acetic acid solution with a pH value of 3.5, stirred at 250 rpm for 1.5 h in a water bath at 85° C., centrifuged at 11000 rpm for 13 min, washed the precipitate with deionized water, and finally dried to obtain cellulose.

[0130] (5) Weigh 10 parts of the roxburghii fruit extract, 3 parts of the zanthoxylum fruit extract, and 0.7 parts of cellulose in corresponding weight portions for later use;

[0131] (6) The roxburghii fruit extract, Zanthoxylum bungeanum fruit extract, and cellulose weighed above are sequentially added into a homogenizer for homogenization to obtain a plant extract.

[0132] A method for preparing a natural plant composition with sunscreen effect comprises the following steps:

[0133] S1, adding plant extract, trehalose and ultrapure water into a stirring tank, stirring and mixing to obtain a first mixed solution;

[0134] S2, heating ethylhexyl methoxycinnamate and wheat germ oil at 85° C. to obtain a second mixed solution;

[0135] S3, adding lecithin to the second mixed solution, after homogenization, adding the first mixed solution, and homogenizing again.

[0136] Comparative Example 5

[0137] A natural plant composition with sunscreen effect, comprising the following raw materials in parts by weight:

[0138] 3 parts of ethylhexyl methoxycinnamate, 26 parts of plant extract, 35 parts of wheat germ oil, 3 parts of lecithin, 4.5 parts of trehalose, and 50 parts of ultrapure water.

[0139] The preparation of the plant extract comprises the following steps:

[0140] (1) placing fresh roxburghii fruit in a microfluidizer at 110 MPa for 2.5 min, soaking and extracting the obtained homogenized product with 70% ethanol for 3.5 times, filtering, combining the extracts, and concentrating the combined extracts by reduced pressure evaporation to obtain a roxburghii fruit extract;

[0141] (2) placing fresh Zanthoxylum bungeanum fruit in a microfluidizer at 110 MPa for 1.5 min, soaking and extracting the obtained homogenized product with 70% ethanol for 3 times, filtering, combining the extracts, and concentrating the combined extracts by evaporation under reduced pressure to obtain a Zanthoxylum bungeanum extract;

[0142] (3) After the freshly picked cherry blossoms are washed with clean water, they are placed in a freeze drying box for vacuum freeze drying, the vacuum degree is controlled to be 17 kPa, the cold trap temperature is -50°C, the holding time is 45 min, and the drying temperature is 9°C. After drying, they are placed in a deep cold mill for pulverization, the temperature is controlled to be -60°C, and the pulverization process is performed for 2.5 min. Then, under the assistance of ultrasound, anhydrous ethanol is used as the extraction solvent for extraction, the stock solution is collected, and the extracted stock solution is concentrated by reduced pressure evaporation at 60°C to obtain cherry blossom extract after concentration;

[0143] (4) Weigh 10 parts of the roxburghii fruit extract, 3 parts of the zanthoxylum fruit extract, and 8 parts of the cherry blossom extract in corresponding weight portions for later use;

[0144] (5) The roxburghii fruit extract, Zanthoxylum bungeanum fruit extract, and cherry blossom extract weighed above are sequentially added into a homogenizer for homogenization to obtain a plant extract.

[0145] A method for preparing a natural plant composition with sunscreen effect comprises the following steps:

[0146] S1, adding plant extract, trehalose and ultrapure water into a stirring tank, stirring and mixing to obtain a first mixed solution;

[0147] S2, heating ethylhexyl methoxycinnamate and wheat germ oil at 85° C. to obtain a second mixed solution;

[0148] S3, adding lecithin to the second mixed solution, after homogenization, adding the first mixed solution, and homogenizing again.

[0149] Performance Testing

[0150] Compositions were prepared according to the methods of Examples 1 to 3 and Comparative Examples 1 to 5 as test samples, and then tested for sunscreen effect and antioxidant efficacy. Specifically:

[0151] 1. Sunscreen effect test

[0152] 1.1 Determination of SPF (Sun protection factor):

[0153] Each group of samples was concentrated at 2 mg / cm 2 Apply an amount of the product onto a transparent ventilation tape, dry for 15 minutes, and then measure using an SPF290 measuring instrument; the SPF value is the average of 3 tests.

[0154] 1.2 Determination of PFA (Protection factor of UVA):

[0155] PA reflects the UVA protection level of sunscreen products. The PA value is represented by "+" and is measured according to the method described in the "Cosmetic Safety Technical Specifications 2015".

[0156] The specific test results are shown in Table 1 below.

[0157] Table 1 Test results of sunscreen performance of natural plant compositions with sunscreen effect in each group

[0158] SPF PA Example 1 65 +++ Example 2 67 ++++ Example 3 64 +++ Comparative Example 1 56 +++ Comparative Example 2 51 ++ Comparative Example 3 57 ++ Comparative Example 4 56 ++ Comparative Example 5 59 +++

[0159] Note: If PFA<2, UVA protection effect shall not be labeled; if 2≤PFA≤3, it shall be labeled PA+; if 4≤PFA≤7, it shall be labeled PA++; if 8≤PFA≤15, it shall be labeled PA+++; if PFA≥16, it shall be labeled PA++++.

[0160] It can be seen from Table 1 above that the sunscreen effect of the compositions of Examples 1 to 3 is significantly better than that of Comparative Examples 1 to 5, and by comparison, it is found that the chemical sunscreen added in the present invention has a synergistic effect with the roxburghii fruit extract, the zanthoxylum fruit extract, the cherry blossom extract, and the cellulose prepared from the pomace of the three plants, and cooperates with each other. The combined use can significantly improve the sunscreen effect of the composition.

[0161] 2. Antioxidant efficacy test

[0162] Each group of compositions was prepared into a 4% aqueous solution with deionized water as a test sample.

[0163] 2.1DPPH free radical scavenging ability

[0164] At room temperature, 100 μL of sample solution and 100 μL of 0.15 mmol / L DPPH solution were mixed and reacted in the dark for 30 min. The absorbance at 517 nm (A s The reaction using the solution instead of the sample was used as the blank (A c ), and the reaction of the solution replacing the DPPH solution as the sample blank (A b Vitamin C and BHT were used as positive controls, and the test was repeated three times. The scavenging rate of DPPH free radicals in the sample was calculated using formula (1), and the half-inhibitory mass concentration (IC 50 ) to evaluate its antioxidant activity, with the unit being mg / mL.

[0165]

[0166] 2.2 Determination of ABTS cation radical scavenging ability

[0167] First prepare the ABTS working solution, then mix 7mmol / L ABTS and 140mmol / L potassium persulfate (volume ratio 625:11), and react in the dark for 12 hours at room temperature. Then, by appropriate dilution, the absorbance of the ABTS working solution at 734nm is about 0.70. The experiment was carried out in a 96-well ELISA plate, and 50μL sample solution and 200μL ABTS working solution were added to each well. After mixing, the mixture was reacted in the dark for 6 minutes. Vitamin C and BHT were used as positive controls, and each experiment was repeated 3 times. The ABTS cation radical scavenging rate of the sample was calculated by measuring the absorbance at 734nm, and IC 50 The value indicates its antioxidant activity.

[0168] The test results are as follows Figure 1 and Figure 2 shown.

[0169] Depend on Figure 1 and Figure 2 It can be seen that the composition prepared by the method of the present invention has good antioxidant activity, strong free radical scavenging ability, and the antioxidant activity is significantly better than that of the comparative example. By comparing the data of the comparative example with the embodiment, it can be concluded that there is a certain synergistic effect between the plant extracts of the present invention, which can synergistically improve the antioxidant activity of the composition.

[0170] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A natural plant composition with sunscreen effect, characterized in that: The invention comprises the following raw materials in parts by weight: 2-4 parts of chemical sunscreen, 20-32 parts of plant extract, 30-40 parts of oil, 2-4 parts of emulsifier, 3-6 parts of moisturizer, 40-60 parts of ultrapure water.

2. A natural plant composition with sunscreen effect according to claim 1, characterized in that: The chemical sunscreen is one or more of ethylhexyl methoxycinnamate, octocrylene, avobenzone, and ethylhexyl triazone.

3. A natural plant composition with sunscreen effect according to claim 1, characterized in that: The preparation of the plant extract comprises the following steps: (1) placing fresh roxburghii fruit in a microfluidizer for high-pressure homogenization, soaking and extracting the obtained homogenized product with 70% ethanol for 3 to 4 times, filtering, combining the extracts and collecting roxburghii pomace, and concentrating the combined extracts by reduced pressure evaporation to obtain a roxburghii fruit extract; (2) placing fresh Zanthoxylum bungeanum fruit in a microfluidizer for high-pressure homogenization, soaking and extracting the obtained homogenized product with 70% ethanol for 3 to 4 times, filtering, combining the extracts and collecting the Zanthoxylum bungeanum pomace, and concentrating the combined extracts by reduced-pressure evaporation to obtain a Zanthoxylum bungeanum extract; (3) After the freshly picked cherry blossoms are washed with clean water, they are placed in a freeze drying box for vacuum freeze drying, and after drying, they are placed in a deep cold mill for pulverization, and then extracted with anhydrous ethanol as an extraction solvent under the assistance of ultrasound, the stock solution and cherry blossom residue are collected, and the extracted stock solution is concentrated by reduced pressure evaporation at 60° C. to obtain a cherry blossom extract after concentration; (4) placing the prickly ash pear residue obtained in step (1), the prickly ash pomace obtained in step (2), and the cherry pomace obtained in step (3) in an oven and drying them to constant weight, respectively, adding them to a grinder in a ratio of 3-4:2-3:1, grinding them, passing through an 80-mesh sieve, adding them to a NaOH solution, hydrolyzing them in a water bath at 80-90° C. for 1-2 h, neutralizing them with hydrochloric acid, centrifuging them at 10,000-12,000 rpm for 10-15 min, washing the precipitate with deionized water by centrifugation for 3-5 times, and then adding them to a glacial acetic acid solution, stirring them in a water bath at 80-90° C. at 200-300 rpm for 1-2 h, centrifuging them at 10,000-12,000 rpm for 10-15 min, washing the precipitate with deionized water by centrifugation, and finally drying them to obtain cellulose; (5) weighing 8 to 12 parts of the roxburghii fruit extract, 2 to 5 parts of the zanthoxylum fruit extract, 6 to 10 parts of the cherry blossom extract, and 0.6 to 0.8 parts of cellulose in corresponding weight portions for later use; (6) The roxburghii fruit extract, Zanthoxylum bungeanum fruit extract, cherry blossom extract, and cellulose weighed above are sequentially added into a homogenizer for homogenization to obtain a plant extract.

4. A natural plant composition with sunscreen effect according to claim 3, characterized in that: The pressure of the high-pressure homogenization treatment in step (1) is 100-120 MPa, and the treatment time is 2-3 min; The pressure of the high-pressure homogenization treatment in step (2) is 100-120 MPa, and the treatment time is 1-2 min.

5. A natural plant composition with sunscreen effect according to claim 3, characterized in that: During the vacuum freeze drying in step (3), the vacuum degree is controlled to be 15 to 20 kPa, the cold trap temperature is -60 to -40°C, the holding time is 30 to 60 minutes, and the drying temperature is 3 to 15°C; During deep cold crushing, the temperature is controlled at -80 to -40°C and the crushing process is 2 to 3 minutes.

6. A natural plant composition with sunscreen effect according to claim 3, characterized in that: The concentration of the NaOH solution in step (4) is 17-21%; The concentration of hydrochloric acid is 35-39%; The pH value of glacial acetic acid solution is 3-4.

7. A natural plant composition with sunscreen effect according to claim 1, characterized in that: The oil is one or more of wheat germ oil, coconut oil, camellia oil and hazelnut oil; The emulsifier is one or more of lecithin, epoxidized castor oil glyceride, and polyoxyethylene lanolin; The moisturizing agent is one or more of trehalose, butylene glycol, propylene glycol and betaine.

8. A method for preparing the natural plant composition according to any one of claims 1 to 7, characterized in that: The steps include: S1, adding the plant extract, moisturizer and ultrapure water into a stirring tank, stirring and mixing to obtain a first mixed solution; S2, heating and mixing the chemical sunscreen and the oil to obtain a second mixed solution; S3, adding the emulsifier to the second mixed solution, homogenizing it, adding the first mixed solution, and homogenizing it again.

9. The method for preparing a natural plant composition with sunscreen effect according to claim 8, characterized in that: The temperature of the heating and mixing in step S2 is 80-90°C.

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