Anti-aging composition, preparation method and application of anti-aging composition in skin aging resistance

Abstract

The invention discloses an anti-aging composition, a preparation method and application of the anti-aging composition in skin aging resistance, belongs to the technical field of cosmetics, and aims at solving the problems that in related technologies, anti-aging cosmetics are instable in functional components, and the skin aging resistance of transdermal absorbability and functional component combination needs to be further improved. The anti-aging composition is prepared from the following components in percentage by total weight of the composition: 10 to 20 percent of an anti-aging functional composition and 80 to 90 percent of an auxiliary material matrix, the composition with the anti-aging effect is prepared from the following main components: ascorbyl tetraisopalmitate, epigallocatechin gallate, hydroxytyrosol, a calendula extract, curcumin, resveratrol and glutathione. The anti-aging composition prepared by the invention has good stability, transdermal absorbability and capability of improving skin aging resistance.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an anti-aging composition, a preparation method and application thereof in anti-skin aging. Background Art

[0002] At present, people have learned that skin aging is affected by multiple factors such as endogenous and exogenous, and is a complex process. With the increase of age, the skin gradually shows problems such as sagging, wrinkles, and dryness. In the environment where people live, some external factors such as ultraviolet rays, blue light, and environmental pollution will also accelerate the skin aging process. At present, there are many anti-aging products on the market, but most of them have problems such as poor transdermal absorption effect, unstable active ingredients, and single efficacy, which cannot meet the needs of consumers for efficient anti-aging products. Although people have learned about some anti-aging substances, it is a difficulty to further efficiently compound effective anti-aging compositions to maximize their efficacy. Therefore, it is of great significance to develop an anti-aging composition that can effectively resist skin aging and has good stability and transdermal absorbability.

[0003] Chinese patent CN113730308B discloses an eye cream composition containing anti-blue light liposomes and its preparation method. In percentage, the eye cream composition containing anti-blue light liposomes includes 1-35% of eye anti-blue light liposomes, 5-40% of emollients, 0.5-30% of emulsifiers, 0.5-20% of fillers, 0.1-10% of thickeners, 0.3-5% of preservatives, 0.01-1% of daily fragrances and deionized water; this invention prepares liposomes with three anti-blue light ingredients to achieve better transdermal absorption and sustained release. The anti-blue light ingredients have strong antioxidant capacity, can remove free radicals generated by blue light irradiation, effectively delay skin aging caused by blue light, reduce pigmentation and spot formation, and repair light-damaged skin to improve skin aging, but the anti-aging effect needs to be further improved. Chinese patent CN108524440B discloses a natural cosmetic emulsion and a preparation method thereof, wherein the raw material components include: 37.6-84.5 parts by weight of aloe vera leaf juice, 10-50 parts by weight of vegetable oil, 5-10 parts by weight of oil foot, 0.1-1 parts by weight of Chondrus crispus extract, 0.1-0.5 parts by weight of Echinacea purpurea extract, 0.1-0.5 parts by weight of rosemary extract, and 0.2-0.4 parts by weight of lactic acid bacteria / rice fermentation product; however, the natural cosmetic emulsion prepared by this invention belongs to an oil-in-water system, has certain protection and repair functions for the skin, and needs to further improve the transdermal absorbability and increase the utilization rate. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides an anti-aging composition, a preparation method and its application in anti-skin aging, which solves the problems in the related art that the effective ingredients of anti-aging cosmetics are unstable, the transdermal absorbability and the effective anti-skin aging performance of the combination of effective ingredients need to be further improved.

[0005] In order to achieve the above object, the present invention provides an anti-aging composition, which is composed of the following components based on the total weight of the composition: 10-20% of an anti-aging efficacy composition and 80-90% of an auxiliary material matrix;

[0006] The anti-aging composition mainly comprises ascorbyl tetraisopalmitate, epigallocatechin gallate, hydroxytyrosol, calendula extract, curcumin, resveratrol and glutathione.

[0007] Preferably, the auxiliary material matrix includes moisturizers, emollients, emulsifiers, thickeners, preservatives, pH adjusters, daily fragrances and water;

[0008] Among them, the mass ratio of moisturizer, emollient, emulsifier, thickener, preservative, pH adjuster, daily fragrance and water is (4-16):(0.8-1.2):(1-9):(7-9):(0.2-0.8):(0.01-0.6):(0.01-0.6):(60-80).

[0009] Preferably, the mass ratio of ascorbyl tetraisopalmitate, epigallocatechin gallate, hydroxytyrosol, calendula extract, curcumin, resveratrol and glutathione is (0.02-0.1):(0.15-0.5):(0.5-2):(0.15-0.5):(0.02-0.1):(0.15-0.5):(0.02-0.1).

[0010] Preferably, the moisturizing agent is any one of glycerin, butylene glycol, sorbitol, xylitol and panthenol.

[0011] Preferably, the emollient is any one of caprylic capric triglyceride, jojoba oil, panthenol, and sodium hyaluronate.

[0012] Preferably, the emulsifier is any one of palmitic acid, stearic acid, sorbitan olivate, and PEG-40 stearate.

[0013] Preferably, the thickener is one or more of hydroxyethyl cellulose, xanthan gum and carbomer.

[0014] Preferably, the preservative is one or more of phenoxyethanol, ethylhexylglycerin, and pentanediol. Preferably, the pH is adjusted by one or more of lactic acid, arginine, and sodium hydroxide.

[0015] The present invention also provides a method for preparing an anti-aging composition, comprising the following steps:

[0016] S1 Preparation of oil phase film: Add ascorbyl tetraisopalmitate, epigallocatechin gallate, curcumin, resveratrol and phospholipids to an organic solvent, stir evenly, and remove the solvent by reduced pressure evaporation on a rotary evaporator to form a uniform oil phase film;

[0017] S2 preparation of internal aqueous phase: dissolving hydroxytyrosol and glutathione in water to prepare the internal aqueous phase;

[0018] S3 Preparation of water-in-oil (O / W) colostrum: slowly add the inner water phase into a container containing an oil phase film, perform hydration treatment at a certain temperature, and then use a high-speed homogenizer to emulsify to form water-in-oil (O / W) colostrum;

[0019] S4 external aqueous phase preparation: dissolving the calendula extract in a weakly acidic buffer to obtain an external aqueous phase;

[0020] Preparation of S5 Anti-Aging Composition:

[0021] Adding water-in-oil (O / W) colostrum to the external water phase and homogenizing to obtain intermediate I;

[0022] Add auxiliary materials to the intermediate I and perform homogenization to obtain an anti-aging composition.

[0023] Preferably, the auxiliary materials include moisturizers, emollients, emulsifiers, thickeners, preservatives, pH adjusters, daily fragrances and water;

[0024] Among them, the mass ratio of moisturizer, emollient, emulsifier, thickener, preservative, pH adjuster, daily fragrance and water is (4-16):(0.8-1.2):(1-9):(7-9):(0.2-0.8):(0.01-0.6):(0.01-0.6):(60-80).

[0025] Preferably, the phospholipid is one or more of lecithin, cephalin, dipalmitole phospholipids, hydrogenated soybean lecithin, and hydrogenated phospholipids.

[0026] Preferably, the mass ratio of ascorbyl tetraisopalmitate, epigallocatechin gallate, curcumin, resveratrol, phospholipid and organic solvent is: (0.02-0.1):(0.15-0.5):(0.02-0.1):(0.15-0.5):(3.5-10):(80-100).

[0027] Preferably, the mass ratio of hydroxytyrosol, glutathione and water is (0.5-2):(0.02-0.1):(8-12).

[0028] Preferably, the mass ratio of the marigold extract to the weakly acidic buffer solution is (0.02-0.1):(4-6).

[0029] Preferably, the organic solvent is ethanol.

[0030] Preferably, the conditions for reduced pressure evaporation are reduced pressure evaporation at 40-50° C. and a pressure of 0.05-0.1 MPa for 30-50 min.

[0031] Preferably, the hydration treatment conditions are: hydration treatment at a temperature of 40-50° C. for 20-40 min.

[0032] Preferably, in S3, the high pressure homogenization condition is: emulsification is performed at a rotation speed of 10000-15000 rpm for 5-10 min.

[0033] Preferably, in S5, the first homogenization condition is: homogenization at a rotation speed of 4000-6000 r / min for 10-15 min.

[0034] Preferably, the weakly acidic buffer is a phosphate buffer.

[0035] Preferably, the pH of the weakly acidic buffer is 6.5-7.

[0036] Preferably, the particle size of the water-in-oil (O / W) colostrum is 50-100 nm.

[0037] The present invention also provides the use of the anti-aging composition described in the above technical solution or the anti-aging composition prepared by the preparation method described in the above technical solution in the preparation of cosmetics.

[0038] The present invention also provides the use of the anti-aging composition described in the above technical solution or the anti-aging composition prepared by the preparation method described in the above technical solution in anti-skin aging.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] 1. The anti-aging ingredients in the present invention include ascorbyl tetraisopalmitate, epigallocatechin gallate, hydroxytyrosol, marigold extract, curcumin, resveratrol and glutathione. Among them, a simple physical mixing and compounding method different from conventional technology is adopted to improve the utilization and biocompatibility of the effective ingredients. The auxiliary matrix in the anti-aging composition provided by the present invention includes glycerol, which can enhance the moisturizing effect, help maintain the moisture balance of the skin, improve the skin condition, and enhance the effects of other ingredients; sodium hyaluronate can increase the moisture content and elasticity of the skin, and synergistically improve the anti-wrinkle effect with other anti-aging ingredients; emulsifiers help to form a stable emulsion system to ensure the uniform distribution and stability of each component; preservatives such as pentylene glycol can prevent microbial contamination and ensure the safety and stability of the product during use.

[0041] 2. The preparation method of the anti-aging composition provided by the present invention is a novel preparation method, wherein hydroxytyrosol and glutathione are water-soluble, and are fully dissolved and dispersed in the aqueous phase to obtain an inner aqueous phase, which is beneficial to stability. Ascorbic acid tetraisopalmitate, epigallocatechin gallate, curcumin, resveratrol and hydrogenated phospholipids are mixed to prepare an oil phase film. For oil-soluble ingredients such as ascorbic acid tetraisopalmitate, epigallocatechin gallate, curcumin and resveratrol, the preparation method of the oil phase film can evenly disperse and encapsulate these oil-soluble anti-aging ingredients in the oil phase, improve their stability, prevent them from being directly exposed to the external environment and oxidized or degraded, and in the subsequent emulsification process, they can be made into an oil-in-water system, increase the transdermal absorption of these ingredients, and make them play a better role. The marigold extract is added to the phosphate buffer to prepare the outer aqueous phase. This specific outer aqueous phase preparation condition helps to maintain the stability and bioavailability of the marigold extract, and better play the role of anti-inflammatory, soothing and repair. The homogenization conditions will further increase stability, allowing the ingredients to fully blend in different phases, ensuring that the oil-soluble and water-soluble anti-aging ingredients are evenly distributed throughout the system, working synergistically to improve the overall efficacy of the product.

[0042] 3. The anti-aging composition prepared by the preparation method of the anti-aging composition of the present invention is a water-in-oil-in-water system, which can simultaneously encapsulate water-soluble and fat-soluble components to form a complex and stable structure, providing better stability and higher bioavailability; and the anti-aging efficacy composition of the present invention has many components, and a preparation method is needed that is suitable for the stable existence of all components and reduces the degradation of the components.

[0043] 4. The anti-aging composition provided in the embodiment of the present invention has significant anti-aging and transdermal absorption effects, and has good stability and safety, slows down skin sagging, wrinkle formation, and promotes skin regeneration, and is a good anti-aging cosmetic. DETAILED DESCRIPTION

[0044] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] Example 1

[0046] This embodiment provides an anti-aging composition, which is composed of the following components based on the total weight of the composition: 10% of the anti-aging efficacy composition and 90% of the auxiliary material matrix;

[0047] Among them, the anti-aging efficacy composition mainly comprises ascorbyl tetraisopalmitate, epigallocatechin gallate, hydroxytyrosol, calendula extract, curcumin, resveratrol and glutathione in a mass ratio of 0.02:0.15:0.5:0.15:0.02:0.15:0.02;

[0048] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the auxiliary material matrix is ​​4:0.8:1:7:0.2:0.01:0.01:60.

[0049] Example 2

[0050] This embodiment provides an anti-aging composition, which is composed of the following components based on the total weight of the composition: 15% of an anti-aging efficacy composition and 85% of an auxiliary material matrix;

[0051] Among them, the anti-aging efficacy composition mainly comprises ascorbyl tetraisopalmitate, epigallocatechin gallate, hydroxytyrosol, calendula officinalis extract, curcumin, resveratrol and glutathione in a mass ratio of 0.06:0.35:1.2:0.35:0.06:0.35:0.06;

[0052] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the auxiliary material matrix is ​​10:1:5:8:0.5:0.3:0.3:70.

[0053] Example 3

[0054] This embodiment provides an anti-aging composition, which is composed of the following components based on the total weight of the composition: 20% of the anti-aging efficacy composition and 80% of the auxiliary material matrix;

[0055] Among them, the anti-aging efficacy composition mainly comprises ascorbyl tetraisopalmitate, epigallocatechin gallate, hydroxytyrosol, calendula extract, curcumin, resveratrol and glutathione in a mass ratio of 0.1:0.5:2:0.5:0.1:0.5:0.1;

[0056] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the auxiliary material matrix is ​​16:1.2:9:9:0.8:0.6:0.6:80.

[0057] Example 4

[0058] This embodiment provides a method for preparing an anti-aging composition, comprising the following steps:

[0059] S1 Preparation of oil phase film: ascorbyl tetraisopalmitate, epigallocatechin gallate, curcumin, resveratrol, hydrogenated phospholipids and ethanol were mixed in a mass ratio of 0.02:0.15:0.02:0.15:3.5:80, stirred evenly, and evaporated under reduced pressure at 40°C and 0.05 MPa on a rotary evaporator for 50 min to remove the solvent, forming a uniform oil phase film;

[0060] S2 Preparation of inner aqueous phase: Mix hydroxytyrosol, glutathione and water in a mass ratio of 0.5:0.02:8 and stir evenly to prepare an inner aqueous phase;

[0061] S3 Preparation of water-in-oil (O / W) colostrum: slowly add the inner water phase into the container containing the oil phase film, hydrate at 40°C for 40 minutes, and then use a high-speed homogenizer to emulsify at a speed of 10,000 rpm for 10 minutes to form water-in-oil (O / W) colostrum;

[0062] S4 preparation of external aqueous phase: mixing the calendula extract and a phosphate buffer solution with a pH of 6.5 at a mass ratio of 0.02:4 to obtain an external aqueous phase;

[0063] Preparation of S5 Anti-Aging Composition:

[0064] Adding water-in-oil (O / W) colostrum to the external water phase, homogenizing at a speed of 4000 r / min for 15 min, to obtain intermediate I;

[0065] Add auxiliary materials to the intermediate I, and homogenize at a speed of 2000 r / min for 8 min to obtain an anti-aging composition;

[0066] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the excipients is 4:0.8:1:7:0.2:0.01:0.01:60;

[0067] Among them, the mass ratio of intermediate I to auxiliary materials is 10:90.

[0068] Example 5

[0069] This embodiment provides a method for preparing an anti-aging composition, comprising the following steps:

[0070] S1 Preparation of oil phase film: ascorbyl tetraisopalmitate, epigallocatechin gallate, curcumin, resveratrol, hydrogenated phospholipids and ethanol were mixed in a mass ratio of 0.06:0.35:0.06:0.35:6.5:90, stirred evenly, and evaporated on a rotary evaporator at 45°C and a pressure of 0.07 MPa for 40 min to remove the solvent, forming a uniform oil phase film;

[0071] S2 Preparation of inner aqueous phase: Mix hydroxytyrosol, glutathione and water in a mass ratio of 1.2:0.06:10 and stir evenly to prepare an inner aqueous phase;

[0072] S3 Preparation of water-in-oil (O / W) colostrum: slowly add the inner water phase into the container containing the oil phase film, hydrate at 45°C for 30 minutes, and then use a high-speed homogenizer to emulsify at 12500 rpm for 7 minutes to form water-in-oil (O / W) colostrum;

[0073] S4 preparation of external aqueous phase: mixing the calendula extract and a phosphate buffer solution with a pH of 6.7 at a mass ratio of 0.06:5 to obtain an external aqueous phase;

[0074] Preparation of S5 Anti-Aging Composition:

[0075] Adding water-in-oil (O / W) colostrum to the external water phase, homogenizing at a speed of 5000 r / min for 13 min, to obtain intermediate I;

[0076] Add auxiliary materials to the intermediate I, and homogenize at a speed of 2500 r / min for 7 minutes to obtain an anti-aging composition;

[0077] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the excipients is 10:1:5:8:0.5:0.3:0.3:70;

[0078] Among them, the mass ratio of intermediate I to auxiliary materials is 15:85.

[0079] Example 6

[0080] This embodiment provides a method for preparing an anti-aging composition, comprising the following steps:

[0081] S1 Preparation of oil phase film: ascorbyl tetraisopalmitate, epigallocatechin gallate, curcumin, resveratrol, hydrogenated phospholipids and ethanol were mixed in a mass ratio of 0.1:0.5:0.1:0.5:10:100, stirred evenly, and evaporated under reduced pressure at 50°C and 0.1 MPa on a rotary evaporator for 30 min to remove the solvent, forming a uniform oil phase film;

[0082] S2 Preparation of inner aqueous phase: Mix hydroxytyrosol, glutathione and water in a mass ratio of 2:0.1:12 and stir evenly to prepare an inner aqueous phase;

[0083] S3 Preparation of water-in-oil (O / W) colostrum: slowly add the inner water phase into the container containing the oil phase film, hydrate at 50°C for 20 minutes, and then use a high-speed homogenizer to emulsify at 15,000 rpm for 5 minutes to form water-in-oil (O / W) colostrum;

[0084] S4 preparation of external aqueous phase: mixing the calendula extract and a phosphate buffer solution having a pH value of 7 at a mass ratio of 0.1:6 to obtain an external aqueous phase;

[0085] Preparation of S5 Anti-Aging Composition:

[0086] Adding water-in-oil (O / W) colostrum to the external water phase, and homogenizing at a speed of 6000 r / min for 10 min to obtain intermediate I;

[0087] Add auxiliary materials to the intermediate I, and homogenize at a speed of 3000 r / min for 6 min to obtain an anti-aging composition;

[0088] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the excipients is 16:1.2:9:9:0.8:0.6:0.6:80;

[0089] Among them, the mass ratio of intermediate I to auxiliary materials is 20:80.

[0090] Comparative Example 1

[0091] This comparative example provides an anti-aging composition, which is composed of the following components based on the total weight of the composition: 10% of the anti-aging efficacy composition and 90% of the auxiliary material matrix;

[0092] Among them, the anti-aging efficacy composition mainly comprises epigallocatechin gallate, hydroxytyrosol, calendula extract, curcumin, resveratrol and glutathione in a mass ratio of 0.15:0.5:0.15:0.02:0.15:0.02;

[0093] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the auxiliary material matrix is ​​4:0.8:1:7:0.2:0.01:0.01:60.

[0094] The preparation method of the anti-aging composition comprises the following steps:

[0095] S1 Preparation of oil phase film: Epigallocatechin gallate, curcumin, resveratrol, hydrogenated phospholipids and ethanol were mixed in a mass ratio of 0.15:0.02:0.15:3.5:80.02, stirred evenly, and evaporated on a rotary evaporator at 40°C and a pressure of 0.05 MPa for 50 min to remove the solvent, forming a uniform oil phase film;

[0096] S2 Preparation of inner aqueous phase: Mix hydroxytyrosol, glutathione and water in a mass ratio of 0.5:0.02:8 and stir evenly to prepare an inner aqueous phase;

[0097] S3 Preparation of water-in-oil (O / W) colostrum: slowly add the inner water phase into the container containing the oil phase film, hydrate at 40°C for 40 minutes, and then use a high-speed homogenizer to emulsify at a speed of 10,000 rpm for 10 minutes to form water-in-oil (O / W) colostrum;

[0098] S4 preparation of external aqueous phase: mixing the calendula extract and a phosphate buffer solution with a pH of 6.5 at a mass ratio of 0.02:4 to obtain an external aqueous phase;

[0099] Preparation of S5 Anti-Aging Composition:

[0100] Adding water-in-oil (O / W) colostrum to the external water phase, homogenizing at a speed of 4000 r / min for 15 min, to obtain intermediate I;

[0101] Add auxiliary materials to the intermediate I, and homogenize at a speed of 2000 r / min for 8 min to obtain an anti-aging composition;

[0102] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the excipients is 4:0.8:1:7:0.2:0.01:0.01:60;

[0103] Among them, the mass ratio of intermediate I to auxiliary materials is 10:90.

[0104] Comparative Example 2

[0105] This comparative example provides an anti-aging composition, which is composed of the following components based on the total weight of the composition: 10% of the anti-aging efficacy composition and 90% of the auxiliary material matrix;

[0106] Among them, the anti-aging efficacy composition mainly comprises hydroxytyrosol, marigold extract, curcumin, resveratrol and glutathione in a mass ratio of 0.5:0.15:0.02:0.15:0.02;

[0107] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the auxiliary material matrix is ​​4:0.8:1:7:0.2:0.01:0.01:60.

[0108] The preparation method of the anti-aging composition comprises the following steps:

[0109] S1 Preparation of oil phase film: Curcumin, resveratrol, hydrogenated phospholipids and ethanol were mixed in a mass ratio of 0.02:0.15:3.5:80.17, stirred evenly, and evaporated on a rotary evaporator at 40°C and a pressure of 0.05 MPa for 50 min to remove the solvent, forming a uniform oil phase film;

[0110] S2 Preparation of inner aqueous phase: Mix hydroxytyrosol, glutathione and water in a mass ratio of 0.5:0.02:8 and stir evenly to prepare an inner aqueous phase;

[0111] S3 Preparation of water-in-oil (O / W) colostrum: slowly add the inner water phase into the container containing the oil phase film, hydrate at 40°C for 40 minutes, and then use a high-speed homogenizer to emulsify at a speed of 10,000 rpm for 10 minutes to form water-in-oil (O / W) colostrum;

[0112] S4 preparation of external aqueous phase: mixing the calendula extract and a phosphate buffer solution with a pH of 6.5 at a mass ratio of 0.02:4 to obtain an external aqueous phase;

[0113] Preparation of S5 Anti-Aging Composition:

[0114] Adding water-in-oil (O / W) colostrum to the external water phase, homogenizing at a speed of 4000 r / min for 15 min, to obtain intermediate I;

[0115] Add auxiliary materials to the intermediate I, and homogenize at a speed of 2000 r / min for 8 min to obtain an anti-aging composition;

[0116] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the excipients is 4:0.8:1:7:0.2:0.01:0.01:60;

[0117] Among them, the mass ratio of intermediate I to auxiliary materials is 10:90.

[0118] Comparative Example 3

[0119] This comparative example provides an anti-aging composition, which is composed of the following components based on the total weight of the composition: 10% of the anti-aging efficacy composition and 90% of the auxiliary material matrix;

[0120] Among them, the anti-aging efficacy composition mainly comprises hydroxytyrosol, calendula extract, resveratrol and glutathione in a mass ratio of 0.5:0.15:0.15:0.02;

[0121] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the auxiliary material matrix is ​​4:0.8:1:7:0.2:0.01:0.01:60.

[0122] The preparation method of the anti-aging composition comprises the following steps:

[0123] S1 Preparation of oil phase film: resveratrol, hydrogenated phospholipids and ethanol were mixed in a mass ratio of 0.15:3.5:80.19, stirred evenly, and evaporated on a rotary evaporator at 40°C and a pressure of 0.05MPa for 50 min to remove the solvent, forming a uniform oil phase film;

[0124] S2 Preparation of inner aqueous phase: Mix hydroxytyrosol, glutathione and water in a mass ratio of 0.5:0.02:8 and stir evenly to prepare an inner aqueous phase;

[0125] S3 Preparation of water-in-oil (O / W) colostrum: slowly add the inner water phase into the container containing the oil phase film, hydrate at 40°C for 40 minutes, and then use a high-speed homogenizer to emulsify at a speed of 10,000 rpm for 10 minutes to form water-in-oil (O / W) colostrum;

[0126] S4 preparation of external aqueous phase: mixing the calendula extract and a phosphate buffer solution with a pH of 6.5 at a mass ratio of 0.02:4 to obtain an external aqueous phase;

[0127] Preparation of S5 Anti-Aging Composition:

[0128] Adding water-in-oil (O / W) colostrum to the external water phase, homogenizing at a speed of 4000 r / min for 15 min, to obtain intermediate I;

[0129] Add auxiliary materials to the intermediate I, and homogenize at a speed of 2000 r / min for 8 min to obtain an anti-aging composition;

[0130] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the excipients is 4:0.8:1:7:0.2:0.01:0.01:60;

[0131] Among them, the mass ratio of intermediate I to auxiliary materials is 10:90.

[0132] Comparative Example 4

[0133] This comparative example provides an anti-aging composition, which is composed of the following components based on the total weight of the composition: 10% of the anti-aging efficacy composition and 90% of the auxiliary material matrix;

[0134] Among them, the anti-aging efficacy composition mainly comprises hydroxytyrosol, marigold extract and glutathione in a mass ratio of 0.5:0.15:0.02;

[0135] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the auxiliary material matrix is ​​4:0.8:1:7:0.2:0.01:0.01:60.

[0136] The preparation method of the anti-aging composition comprises the following steps:

[0137] S1 Preparation of oil phase film: hydrogenated phospholipid and ethanol were mixed in a mass ratio of 3.5:80.34, stirred evenly, and evaporated on a rotary evaporator at 40°C and a pressure of 0.05 MPa for 50 min to remove the solvent, forming a uniform oil phase film;

[0138] S2 Preparation of inner aqueous phase: Mix hydroxytyrosol, glutathione and water in a mass ratio of 0.5:0.02:8 and stir evenly to prepare an inner aqueous phase;

[0139] S3 Preparation of water-in-oil (O / W) colostrum: slowly add the inner water phase into the container containing the oil phase film, hydrate at 40°C for 40 minutes, and then use a high-speed homogenizer to emulsify at a speed of 10,000 rpm for 10 minutes to form water-in-oil (O / W) colostrum;

[0140] S4 preparation of external aqueous phase: mixing the calendula extract and a phosphate buffer solution with a pH of 6.5 at a mass ratio of 0.02:4 to obtain an external aqueous phase;

[0141] Preparation of S5 Anti-Aging Composition:

[0142] Adding water-in-oil (O / W) colostrum to the external water phase, homogenizing at a speed of 4000 r / min for 15 min, to obtain intermediate I;

[0143] Add auxiliary materials to the intermediate I, and homogenize at a speed of 2000 r / min for 8 min to obtain an anti-aging composition;

[0144] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the excipients is 4:0.8:1:7:0.2:0.01:0.01:60;

[0145] Among them, the mass ratio of intermediate I to auxiliary materials is 10:90.

[0146] Comparative Example 5

[0147] This comparative example provides an anti-aging composition, which is composed of the following components based on the total weight of the composition: 10% of the anti-aging efficacy composition and 90% of the auxiliary material matrix;

[0148] Among them, the anti-aging efficacy composition mainly comprises hydroxytyrosol and glutathione in a mass ratio of 0.5:0.02;

[0149] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the auxiliary material matrix is ​​4:0.8:1:7:0.2:0.01:0.01:60.

[0150] The preparation method of the anti-aging composition comprises the following steps:

[0151] S1 Preparation of oil phase film: hydrogenated phospholipid and ethanol were mixed in a mass ratio of 3.5:80.34, stirred evenly, and evaporated on a rotary evaporator at 40°C and a pressure of 0.05 MPa for 50 min to remove the solvent, forming a uniform oil phase film;

[0152] S2 Preparation of inner aqueous phase: Mix hydroxytyrosol, glutathione and water in a mass ratio of 0.5:0.02:8 and stir evenly to prepare an inner aqueous phase;

[0153] S3 Preparation of water-in-oil (O / W) colostrum: slowly add the inner water phase into the container containing the oil phase film, hydrate at 40°C for 40 minutes, and then use a high-speed homogenizer to emulsify at a speed of 10,000 rpm for 10 minutes to form water-in-oil (O / W) colostrum;

[0154] Preparation of S4 Anti-Aging Composition:

[0155] The water-in-oil (O / W) colostrum was homogenized at a speed of 4000 r / min for 15 min to obtain intermediate I;

[0156] Add auxiliary materials to the intermediate I, and homogenize at a speed of 2000 r / min for 8 min to obtain an anti-aging composition;

[0157] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the excipients is 4:0.8:1:7:0.2:0.01:0.01:60;

[0158] Among them, the mass ratio of intermediate I to auxiliary materials is 10:90.

[0159] Comparative Example 6

[0160] This comparative example provides an anti-aging composition, which is composed of the following components based on the total weight of the composition: 10% of the anti-aging efficacy composition and 90% of the auxiliary material matrix;

[0161] Among them, the anti-aging efficacy composition, the main component is hydroxytyrosol;

[0162] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the auxiliary material matrix is ​​4:0.8:1:7:0.2:0.01:0.01:60.

[0163] The preparation method of the anti-aging composition comprises the following steps:

[0164] S1 Preparation of oil phase film: hydrogenated phospholipid and ethanol were mixed in a mass ratio of 3.5:80.34, stirred evenly, and evaporated on a rotary evaporator at 40°C and a pressure of 0.05 MPa for 50 min to remove the solvent, forming a uniform oil phase film;

[0165] S2 Preparation of internal aqueous phase: Mix hydroxytyrosol and water in a mass ratio of 0.5:8.02, stir evenly, and prepare an internal aqueous phase;

[0166] S3 Preparation of water-in-oil (O / W) colostrum: slowly add the inner water phase into the container containing the oil phase film, hydrate at 40°C for 40 minutes, and then use a high-speed homogenizer to emulsify at a speed of 10,000 rpm for 10 minutes to form water-in-oil (O / W) colostrum;

[0167] Preparation of S4 Anti-Aging Composition:

[0168] The water-in-oil (O / W) colostrum was homogenized at a speed of 4000 r / min for 15 min to obtain intermediate I;

[0169] Add auxiliary materials to the intermediate I, and homogenize at a speed of 2000 r / min for 8 min to obtain an anti-aging composition;

[0170] Among them, the mass ratio of glycerin, sodium hyaluronate, stearic acid, xanthan gum, pentylene glycol, lactic acid, daily flavor and water in the excipients is 4:0.8:1:7:0.2:0.01:0.01:60;

[0171] Among them, the mass ratio of intermediate I to auxiliary materials is 10:90.

[0172] In the present invention, ascorbyl tetraisopalmitate comes from Shanxi Ruisi Biotechnology Co., Ltd.; epigallocatechin gallate comes from Xi'an Changhong Biotechnology Co., Ltd.; hydroxytyrosol comes from Xi'an Qiuhe Biotechnology Co., Ltd.; curcumin comes from Shaanxi Haoyun Pharmaceutical Technology Co., Ltd.; resveratrol comes from Hubei Sanxin Biotechnology Co., Ltd.; glutathione comes from Xi'an Meihe Biotechnology Co., Ltd.; demulsifier comes from Huainan Kedi Chemical Technology Co., Ltd., model PE2040; others are commercially available products.

[0173] The anti-aging compositions prepared in Example 1 and Comparative Examples 1-6 were selected as samples, respectively named as Samples 1-7, and the properties of Samples 1-7 were tested accordingly. The test methods and test results are as follows;

[0174] (1) In vitro transdermal test: A vertical Franz diffusion cell method was used to conduct an in vitro mouse skin transdermal test, specifically: the abdominal skin of SD male rats was fixed between the receiving chamber and the donor chamber, 1 g of each sample 1-7 was taken into the donor chamber, 15% ethanol, 5% Tween 80 and 80% saline were used as the receiving solution, and the mixture was stirred and diffused at 37°C, and an equal amount of constant temperature fresh receiving solution was immediately added; after 12 hours, the skin was removed, washed, cut into pieces, ground into a homogenate, an appropriate amount of receiving solution was added, centrifuged, and the supernatant was taken for HPLC analysis to calculate the specific hydroxytyrosol retention per unit area of ​​the skin; the specific test results are shown in Table 1;

[0175] Table 1

[0176]

[0177] As shown in the test results of Table 1, the cumulative skin permeation of sample 1 after 12 hours is higher than that of samples 2-7. This is mainly because the anti-aging composition prepared in comparative example 1 is a water-in-oil-in-water substance, and hydroxytyrosol is in the inner water phase, which is more stable and has a small particle size, which is conducive to having stronger adhesion and biocompatibility with cells, so that the active substance can more effectively penetrate the skin surface to reach the dermis. For oil-soluble ingredients such as ascorbyl tetraisopalmitate, epigallocatechin gallate, curcumin and resveratrol, the preparation method of the oil phase film can evenly disperse and wrap these oil-soluble anti-aging ingredients in the oil phase, improve their stability, and can be made into an oil-in-water system in the subsequent emulsification process, increasing the transdermal absorption of these ingredients. Calendula officinalis extract has the ability to regulate the connection and lipid structure between skin cells, thereby temporarily expanding the intercellular space, facilitating the penetration and retention of other ingredients, and producing a synergistic effect with other ingredients in the formula, thereby improving the penetration and retention effect of the skin. Once lacking, the transdermal absorption performance is naturally reduced.

[0178] (2) Stability test: Samples 1-7 were placed at 50°C, room temperature, and -10°C for 12 months, respectively, and the properties of the samples were observed. The content of hydroxytyrosol in each sample was detected by high performance liquid chromatography (HPLC), and the residual mass content percentage of hydroxytyrosol after 12 months was calculated to comprehensively evaluate the stability. The specific test results are shown in Table 2.

[0179] Table 2

[0180]

[0181] From the test results in Table 2, it can be seen that Example 1 has good stability.

[0182] (3) Antioxidant performance test: 3 mL of 0.0178 mmol / L DPPH-anhydrous methanol solution, 1 L of demulsifier and 3 mL of sample 1-7 were mixed evenly, centrifuged at 5000 r / min for 15 s, and the supernatant was taken to measure its absorbance at a wavelength of 517 nm, and the absorbance value was recorded as A1. After stirring at 150 r / min for 30 min at 37°C in the dark, the mixture was centrifuged at 5000 r / min for 15 s, and the supernatant was taken to measure its absorbance at a wavelength of 517 nm, and the absorbance value was recorded as A2. The blank control was only added with DPPH-anhydrous methanol solution, and its absorbance value was recorded as A3. DPPH free radical scavenging rate (%) = [1-(A1-A2) / A3] x 100%. Each group of samples was tested 3 times and the average value was taken. The specific results are shown in Table 3.

[0183] Table 3

[0184]

[0185] It can be seen from the test results in Table 3 that the anti-aging composition prepared by the present invention has the best antioxidant capacity. Among them, ascorbyl tetraisopalmitate in sample 1 can effectively capture free radicals, and as its concentration increases, the free radical scavenging rate usually gradually increases; epigallocatechin gallate has a strong ability to directly scavenge free radicals; hydroxytyrosol shows a high affinity for free radicals, and an increase in its content usually leads to an increase in the free radical scavenging rate; the active ingredients in the calendula extract can participate in the free radical scavenging process directly or indirectly, and an increase in the amount of the extract will increase the free radical scavenging rate; resveratrol can activate the antioxidant mechanism inside the cell, thereby enhancing the free radical scavenging ability, and as the concentration of resveratrol increases, the free radical scavenging rate will increase; glutathione, as an antioxidant, has a certain free radical scavenging ability; curcumin contains multiple phenolic hydroxyl groups in its chemical structure, which enables it to effectively capture and stabilize free radicals and reduce their damage to cells and tissues. Once the above substances are missing, the antioxidant properties will naturally decrease.

[0186] (4) Skin anti-wrinkle effect test: A total of 70 subjects with normal healthy skin and no history of cosmetic allergies were selected. The subjects were aged 20-35 years old and were divided into 7 groups, with 10 subjects in each group. The cheeks of the subjects were selected as the test area. The six groups of subjects used samples 1-7 respectively. The subjects were required to clean their faces before applying the samples, and apply the corresponding samples after the skin was dried. Each person applied the corresponding samples twice a day, 1g each time, for 6 consecutive weeks. During the experiment, the subjects were not allowed to apply any other products except the tested samples on the experimental sites. The Micro SkinⅡ multifunctional dermatoscope image analysis system was used to measure the changes in skin roughness of the applied sites after 0, 2, 4, and 6 weeks after the subjects used the samples. The average value of skin roughness was taken for each group. The test results are shown in Table 4:

[0187] Table 4

[0188]

[0189] According to the test results in Table 4, it can be seen that within the 6-week test period, the skin roughness of the test site after using samples 1-7 showed a decreasing trend, and the anti-wrinkle effect of Example 1 was better than that of Comparative Examples 1-6. Ascorbyl tetraisopalmitate in Sample 1 can inhibit the activity of collagenase and reduce the decomposition of collagen; at the same time, it promotes the proliferation and differentiation of fibroblasts and increases the production of collagen; epigallocatechin gallate can inhibit the activity of matrix metalloproteinases (MMPs), reduce the destruction of collagen and elastic fibers, and prevent and reduce wrinkles; hydroxytyrosol with antioxidant effect can reduce the damage of oxidative stress to skin collagen and elastic fibers, help maintain the structure and elasticity of the skin, and reduce wrinkles; calendula extract has anti-inflammatory and soothing effects, reduces skin damage caused by inflammation, promotes skin repair and regeneration, and improves wrinkle conditions; curcumin can help repair wrinkles by reducing inflammatory reactions and reducing oxidative damage; resveratrol can enhance the self-repair ability of cells, thereby reducing wrinkles and facilitating anti-aging performance; once it is lacking, the anti-wrinkle effect is naturally reduced. At the same time, ascorbyl tetraisopalmitate, epigallocatechin gallate, curcumin, resveratrol, hydrogenated phospholipids and ethanol in sample 1 are mixed, and then the solvent is removed to obtain an oil phase. The hydrogenated phospholipids in it have the effects of promoting penetration, moisturizing and anti-aging; once it is lacking, the anti-wrinkle effect will naturally decrease.

[0190] (5) Human skin patch test: 70 subjects with normal healthy skin and no history of cosmetic allergy were selected, aged 20-35 years old, and divided into 7 groups, with 10 subjects in each group. The mildness test was carried out according to the "Cosmetic Safety Technical Specifications 2015 Edition" - Human Skin Patch Test. According to GB 171492-1997 "Diagnostic Criteria and Treatment Principles for Cosmetic Contact Dermatitis", it was confirmed that the subjects had no serious systemic diseases, no immunodeficiency or autoimmune diseases, no active allergic diseases, no history of allergy to skin care cosmetics, no systemic use of hormone drugs and immunosuppressants within 1 month, no pregnancy or lactation, and no ethical contraindications. Samples 1-7 were patch tested respectively, and the patch tester was blank. Using the closed patch test method, about 0.020m of the test substance is placed in the patch tester, and a low-allergenic tape is applied to the back of the subject. The test substance is removed after 24 hours, and the skin reaction is observed once at 0h, 0.5h, 12h, 24h and 48h respectively; the reaction results are recorded according to the grading standard: Grade 0 is a negative reaction, Grade 1 is a suspicious reaction, with only weak erythema; Grade 2 is a weak positive reaction, with erythema, infiltration, edema, and papules; Grade 3 is a strong positive reaction, with erythema, infiltration, edema, papules, and herpes reactions that may exceed the test area; Grade 4 is an extremely strong positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area; the test results are shown in Table 5;

[0191] Table 5

[0192]

[0193]

[0194] It can be seen from the test results in Table 5 that the anti-aging compositions in Example 1 and Comparative Examples 1-6 have high safety performance.

[0195] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that all equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the patent coverage of the present invention.

Claims

1. An anti-aging composition, characterized in that The anti-aging composition is composed of the following components based on the total weight of the composition: 10-20% of the anti-aging efficacy composition and 80-90% of the auxiliary material matrix; The anti-aging composition mainly comprises ascorbyl tetraisopalmitate, epigallocatechin gallate, hydroxytyrosol, calendula extract, curcumin, resveratrol and glutathione.

2. An anti-aging composition according to claim 1, characterized in that: The excipient matrix includes moisturizers, emollients, emulsifiers, thickeners, preservatives, pH adjusters, daily fragrances and water; Among them, the mass ratio of moisturizer, emollient, emulsifier, thickener, preservative, pH adjuster, daily fragrance and water is (4-16):(0.8-1.2):(1-9):(7-9):(0.2-0.8):(0.01-0.6):(0.01-0.6):(60-80).

3. An anti-aging composition according to claim 1, characterized in that: The mass ratios of ascorbyl tetraisopalmitate, epigallocatechin gallate, hydroxytyrosol, calendula officinalis extract, curcumin, resveratrol and glutathione are (0.02-0.1):(0.15-0.5):(0.5-2):(0.15-0.5):(0.02-0.1):(0.15-0.5):(0.02-0.1).

4. The method for preparing the anti-aging composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1 Preparation of oil phase film: Add ascorbyl tetraisopalmitate, epigallocatechin gallate, curcumin, resveratrol and phospholipids to an organic solvent, stir evenly, and remove the solvent by reduced pressure evaporation on a rotary evaporator to form a uniform oil phase film; S2 preparation of internal aqueous phase: dissolving hydroxytyrosol and glutathione in water to prepare the internal aqueous phase; S3 Preparation of water-in-oil (O / W) colostrum: slowly add the inner water phase into a container containing an oil phase film, perform hydration treatment at a certain temperature, and then use a high-speed homogenizer to emulsify to form water-in-oil (O / W) colostrum; S4 external aqueous phase preparation: dissolving the calendula extract in a weakly acidic buffer to obtain an external aqueous phase; Preparation of S5 Anti-Aging Composition: The water-in-oil (O / W) colostrum is added to the external water phase, homogenized, and a moisturizer, an emollient, an emulsifier, a thickener, a preservative, a pH adjuster, a daily fragrance and water are added, and homogenized to obtain an anti-aging composition.

5. The preparation method according to claim 4, characterized in that: The phospholipids are one or more of lecithin, cephalin, dipalmitole lecithin, hydrogenated soybean lecithin, and hydrogenated phospholipids.

6. The preparation method according to claim 4, characterized in that: The mass ratios of ascorbyl tetraisopalmitate, epigallocatechin gallate, curcumin, resveratrol, phospholipids and organic solvent are: (0.02-0.1):(0.15-0.5):(0.02-0.1):(0.15-0.5):(3.5-10):(80-100).

7. The preparation method according to claim 4, characterized in that: The mass ratio of hydroxytyrosol, glutathione and water is (0.5-2):(0.02-0.1):(8-12).

8. The preparation method according to claim 4, characterized in that: The mass ratio of the marigold extract to the weakly acidic buffer solution is (0.02-0.1):(4-6).

9. Use of the anti-aging composition according to any one of claims 1 to 3 or the anti-aging composition prepared by the preparation method according to any one of claims 4 to 8 in the preparation of cosmetics.

10. Use of the anti-aging composition according to any one of claims 1 to 3 or the anti-aging composition prepared by the preparation method according to any one of claims 4 to 8 in anti-skin aging.

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