Guaiai extract-containing composition for improving female acne in menstrual period and application of guaiai extract-containing composition

Abstract

The invention relates to a guaiacol extract-containing composition for improving menstrual female acnes and application thereof, the composition is formed by combining five components including a mango leaf extract, a myrtle fruit extract, 10-hydroxydecanoic acid, a prepared rehmannia root extract and a guaiacol extract, and the five components have a synergistic effect; lipid oxidation can be effectively inhibited, migration of skin keratinocyte is promoted, and the effects of dredging sebaceous glands and preventing acne are achieved; in addition, 5-alpha reductase can be inhibited, so that stimulation of male hormone is reduced, and the situation that sebum secretion is increased due to menstrual hormone fluctuation is reduced; in addition, propionibacterium acnes can be inhibited, and the effects of preventing acne and improving existing acnes are achieved; in addition, the composition disclosed by the invention is fragrant in smell, and can relieve the dysphoria of women in the menstrual period and indirectly improve the skin condition. Therefore, when the composition is applied to cosmetics, facial acnes of menstrual female acne subjects can be improved, and the dysphoria mood of menstrual female can be relieved.

Description

Technical Field

[0001] The invention relates to the technical field of skin care products, and in particular to a composition containing a guaiac extract for improving acne in women during menstruation and an application thereof. Background Art

[0002] Menstrual acne is a common problem for many women during their menstrual cycle. Its main characteristics are that it is usually accompanied by a significant increase in the number of acne from the week before menstruation to the beginning of menstruation. There are various types of menstrual acne, mainly including inflammatory acne and comedonal lesions, and it usually gradually eases within a week after the end of menstruation. During the luteal phase of the menstrual cycle, the secretion of estrogen and androgen increases, leading to increased sebum secretion. It has been reported that during this period, the sebaceous gland ducts of the skin will become narrow, making it easy for sebum to accumulate, thus forming and aggravating acne. In addition, the decline in skin barrier function before menstruation may also aggravate acne problems due to increased bacterial growth. Mood swings during menstruation are also an important factor in aggravating acne. Women often feel anxious, nervous or depressed due to hormonal changes during menstruation, which not only affects their psychological state, but may also lead to increased sebum secretion and worsening skin acne conditions. Therefore, the difficulty in treating menstrual acne lies in its hormone dependence and the interaction of emotional factors, making targeted treatment plans more complicated. Unstable hormone levels, structural changes in sebaceous glands, and mood swings can affect the function of sebaceous glands, leading to the continued appearance of acne. In addition, when women face premenstrual discomfort and emotional challenges, they may neglect skin care, further aggravating skin problems.

[0003] The treatment of menstrual acne is of great significance. First of all, it is not only related to the health of the skin, but also has a direct impact on mental health and self-confidence. The anxiety and inferiority complex caused by acne in women often affect their daily life and social activities. Therefore, timely and effective treatment can improve skin condition and reduce psychological burden. The products currently on the market do not pay attention to the demand for menstrual acne. Most acne-removing products only use powerful ingredients such as salicylic acid, glycolic acid or zinc oxide to remove acne, which will aggravate the unstable state of the skin during menstruation. The stinging pain will also increase the anxiety of women during menstruation. Therefore, it is necessary to develop acne care compositions specifically for women during menstruation. Summary of the invention

[0004] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a composition containing guaiac extract for improving acne in women during menstruation and its application.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] In a first aspect, the present invention provides a composition for improving acne in women during menstruation containing a guaiac extract, the composition being composed of the following components: mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract, wherein the weight ratio of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is (0.1-5): (0.1-5): (0.01-1): (0.1-3): (0.1-3).

[0007] For the treatment of women with menstrual acne, mango leaf extract has shown a significant effect in inhibiting sebum oxidation. During menstruation, the fluctuation of hormone levels in women's bodies may lead to increased sebum secretion, which in turn forms oil that accumulates in the pores and causes acne. Mango leaf extract, through its powerful antioxidant properties, effectively reduces sebum oxidation, reduces the activity of sebaceous glands, and thus inhibits the formation of acne, thus providing a natural skin care treatment option for women with menstrual acne.

[0008] Myrtle fruit extract plays an important role in anti-inflammatory, soothing and maintaining the balance of skin microecology. The occurrence of menstrual acne is often accompanied by skin inflammation and the proliferation of Propionibacterium acnes, a bacterium that is harmful to the sebaceous glands. Myrtle fruit extract reduces skin inflammation through anti-inflammatory mechanisms and has the effect of inhibiting harmful types IA1 and IA2 of Propionibacterium acnes, reducing its damage to the sebaceous glands. In addition, it also helps to balance the skin microbiome and prevent the overgrowth of bad bacteria, thereby further improving the skin condition.

[0009] 10-Hydroxydecanoic acid, also known as royal jelly acid, is a unique fatty acid extracted from royal jelly and has antibacterial, anti-inflammatory and anti-aging effects. Its ability is not only to refine pores and smooth skin, but also to reduce oil secretion and inhibit the overactivity of sebaceous gland cells, achieving a dual effect of improving skin texture and reducing the formation of acne.

[0010] Rehmannia glutinosa extract inhibits the activity of 5-α reductase, reduces the effects of androgens, and thus inhibits acne caused by hormone fluctuations. At the same time, its anti-inflammatory properties also help relieve the inflammatory response caused by hormone fluctuations, further promoting skin health.

[0011] Guaiac wood extract is also very effective in soothing and anti-inflammatory aspects. Its unique aromatic plant ingredients can not only soothe skin conditions, but also help women achieve a balance between body and mind during menstruation by regulating emotions. This mood-soothing effect can indirectly improve skin conditions by reducing the emotional stress caused by menstrual fluctuations, and has a positive effect on promoting skin conditions.

[0012] In summary, the composition of the present invention is composed of five components, namely, mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and guaiac wood extract. These five components work synergistically to significantly inhibit the production of sebum oxidation markers (lipid peroxides LPO), because sebum is secreted more during menstruation, and oxidized sebum is more likely to clog sebaceous glands, which is a challenge to the narrow sebaceous glands during menstruation. Therefore, anti-sebum oxidation can help dredge sebaceous glands and prevent acne; promote the migration of skin keratinocytes; and have a significant inhibitory effect on 5-α reductase, which can greatly reduce the stimulation of androgen. In addition, the composition can also inhibit Propionibacterium acnes and regulate emotions. Therefore, applying the composition to cosmetics can improve facial acne in female acne subjects during menstruation, and relieve the irritable emotions of women during menstruation.

[0013] Preferably, the weight ratio of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is (1-3): (1-2): (0.01-0.5): (0.5-3): (1-3).

[0014] Preferably, the weight ratio of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is (1.5-2.5): (1.5-2): (0.05-0.1): (0.5-1): (2.5-3).

[0015] In a second aspect, the present invention provides the use of the composition for improving acne in menstruating women containing the guaiac extract in the preparation of skin care products.

[0016] Preferably, the skin care product is at least one of lotion, emulsion, cream, mask, essence or spray, and the added amount of the composition is 0.5%-5% of the total weight of the skin care product.

[0017] In a third aspect, the present invention provides an essence comprising the following raw materials in weight percentage: 0.5%-5% of the composition for improving acne in menstruating women containing guaiac extract in the first aspect, 0.05%-0.3% of a thickener, 0.5%-5% of a moisturizer, 0.5%-3% of a preservative, 0.01%-0.3% of a pH adjuster and 0.01%-1% of a fragrance, and the balance being deionized water.

[0018] Preferably, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol.

[0019] Preferably, the moisturizing agent includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, hydrolyzed sodium hyaluronate, 1,2-butylene glycol, glycerol, budding pullulan, and ceramide. More preferably, glycerol, 1,2-butylene glycol, and hydrolyzed sodium hyaluronate.

[0020] Preferably, the pH regulator comprises at least one of arginine, tromethamine, and EDTA-disodium, more preferably arginine.

[0021] Preferably, the preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone, more preferably 1,2-hexanediol and p-hydroxyacetophenone.

[0022] In a fourth aspect, the present invention provides a method for preparing the essence of the third invention, comprising the following steps:

[0023] S1. Mix the moisturizer, thickener and part of deionized water, homogenize at 75-85°C, cool to 55-65°C, add the preservative, stir evenly, and obtain a mixture;

[0024] S2. When the temperature drops to 35-45° C., add the components in the composition and the remaining deionized water to the mixture obtained in step S1, stir evenly, and finally add a pH adjuster to adjust the pH to obtain the essence.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The composition of the present invention is composed of five ingredients, namely, mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract. The five ingredients have a synergistic effect, can effectively inhibit lipid oxidation and promote the migration of skin keratinocytes, play the role of unblocking sebaceous glands and preventing acne; and can also inhibit 5-α reductase to reduce the stimulation of male hormones, thereby reducing the increase in sebum secretion caused by menstrual hormone fluctuations; and can inhibit Propionibacterium acnes, play the role of preventing acne and improving existing acne; in addition, the composition of the present invention has a fresh smell, can soothe the irritability of women during menstruation, and indirectly improve skin conditions. Therefore, the application of the composition to cosmetics can improve facial acne in female acne subjects during menstruation, and soothe the irritability of women during menstruation. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The VISA graphs of the skin of the subjects who used the essences of Application Example 1 and Comparative Application Example 6 at 0 day and 28 day were tested. DETAILED DESCRIPTION

[0028] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0029] The sources of the components used in the following examples and comparative examples are as follows:

[0030] Mango leaf extract: Manufacturer: Givaudan, brand: Mangixyl TM ;

[0031] Myrtle fruit extract: The manufacturer is France Green Natural Personal Care Products Co., Ltd., the brand is

[0032] 10-Hydroxydecanoic acid: The manufacturer is Beijing Dongfang Miaosen Biotechnology Co., Ltd.;

[0033] Rehmannia glutinosa extract: the manufacturer is Youran Biotechnology Co., Ltd.;

[0034] Guaiac wood extract: The manufacturer is Zhenghe (Guangzhou) Biotechnology Co., Ltd.

[0035] Unless otherwise specified, other materials, reagents, etc. used in the examples and comparative examples can be obtained from commercial sources.

[0036] Example 1

[0037] A composition for improving acne in women during menstruation containing guaiac extract, comprising mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract in a weight ratio of 2:1.8:0.07:0.6:2.6, wherein the total weight of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is 100 parts.

[0038] Example 2

[0039] A composition for improving acne in women during menstruation containing guaiac extract, comprising mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract in a weight ratio of 1.5:2:0.1:0.5:3, wherein the total weight proportion of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is 100 parts.

[0040] Example 3

[0041] A composition for improving acne in women during menstruation containing guaiac extract, comprising mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract in a weight ratio of 2.5:1.5:0.05:1:2.5, wherein the total weight proportion of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is 100 parts.

[0042] Example 4

[0043] A composition for improving acne in women during menstruation containing guaiac extract, comprising mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract in a weight ratio of 1:2; 0.01:3:1, wherein the total weight proportion of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is 100 parts.

[0044] Example 5

[0045] A composition for improving acne in women during menstruation containing guaiac extract, comprising mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract in a weight ratio of 3:1:0.5:0.5:3, wherein the total weight proportion of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is 100 parts.

[0046] Example 6

[0047] A composition for improving acne in women during menstruation containing guaiac extract, comprising mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract in a weight ratio of 0.1:5:0.01:0.1:3, wherein the total weight proportion of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is 100 parts.

[0048] Example 7

[0049] A composition for improving acne in women during menstruation containing guaiac extract, comprising mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract in a weight ratio of 5:0.1:1:3:0.1, wherein the total weight of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is 100 parts.

[0050] Comparative Example 1

[0051] The difference between Comparative Example 1 and Example 1 is that the total weight of the composition remains unchanged, mango leaf extract is not added, and a composition of myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract in a weight ratio of 1.8:0.07:0.6:2.6 is used to make up for the missing amount.

[0052] Comparative Example 2

[0053] The difference between Comparative Example 2 and Example 1 is that the total weight of the composition remains unchanged, myrtle fruit extract is not added, and a composition of mango leaf extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract in a weight ratio of 2:0.07:0.6:2.6 is used to make up for the missing amount.

[0054] Comparative Example 3

[0055] The difference between Comparative Example 3 and Example 1 is that the total weight of the composition remains unchanged, 10-hydroxydecanoic acid is not added, and a composition of mango leaf extract, myrtle fruit extract, rehmannia root extract and guaiac extract in a weight ratio of 2:1.8:0.6:2.6 is used to make up for the missing amount.

[0056] Comparative Example 4

[0057] The difference between Comparative Example 4 and Example 1 is that the total weight of the composition remains unchanged, no Rehmannia root extract is added, and a composition of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid and guaiac extract in a weight ratio of 2:1.8:0.07:2.6 is used to make up for the missing amount.

[0058] Comparative Example 5

[0059] The difference between Comparative Example 5 and Example 1 is that the total weight of the composition remains unchanged, no guaiac extract is added, and a composition of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid and Rehmannia glutinosa extract in a weight ratio of 2:1.8:0.07:0.6 is used to make up for the missing amount.

[0060] Test Example 1: Lipid oxidation inhibition rate test

[0061] Lipid peroxides (LPO) are oxidation products produced by the reaction of lipids with reactive oxygen species, and are often considered a sign of cell membrane damage and oxidative stress. In the pathogenesis of acne, the production of LPO is closely related to inflammatory responses, sebaceous gland dysfunction, and bacterial infection. High levels of LPO usually reflect increased oxidative stress in the skin, which in turn triggers an increase in inflammatory responses, especially in the skin of women during menstruation, where not only are oxidized lipids more likely to appear, but the sebaceous glands become narrower, making it more likely for sebaceous glands to become blocked, leading to the occurrence and deterioration of acne. Therefore, the level of LPO can be used as an important indicator to assess the severity of acne.

[0062] The compositions for improving acne in women during menstruation containing the guaiac extract of Examples 1-7 and the compositions of Comparative Examples 1-5 were subjected to experiments according to the following steps. The results are shown in Table 1.

[0063] The human sebaceous gland cell SZ95 was revived under the following test conditions: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%; the cells were cultured and treated according to the grouping, and then tested. The specific test was the detection of lipid peroxide LPO content. The test method was as follows:

[0064] (1) Recovery: The cell suspension was inoculated into a 96-well cell culture plate at a density of 2000 cells per well. 100 μL of DMEM high-glucose medium containing 10% fetal bovine serum + 1% double antibody solution (Gibco) was added to each well and cultured for 24 h.

[0065] (2) After the cells were revived, the original culture medium was discarded, 100 μL of culture medium containing 10 mM hydrocortisone was added to the control group, and 100 μL of culture medium containing 0.5 wt % composition + 10 mM hydrocortisone was added to each of the 12 sample groups, and the culture was continued for 24 h;

[0066] (3) Lipid peroxide (LPO) detection: The lipid peroxide (LPO) ELISA detection kit was used to detect the LPO content of the control group and the LPO content of each sample group. The inhibitory effect of the sample on LPO is expressed as the LPO inhibition rate, and the calculation formula is as follows:

[0067] LPO inhibition rate (%) = (LPO content in control group - LPO content in sample group) / LPO content in control group × 100%.

[0068] Table 1 LPO inhibition rate data of each group

[0069]

[0070]

[0071] From the data of Example 1 and Comparative Examples 1-5 in Table 1, it can be seen that the composition of Example 1, which is a compound of five ingredients including mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract, has a significantly improved LPO inhibition rate compared with the compositions of Comparative Examples 1-5, indicating that the five ingredients of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract can synergistically improve the effect of dredging sebaceous glands and preventing acne.

[0072] Combined with the data of Examples 1-7, it can be seen that when the weight ratio of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract is (1.5-2.5): (1.5-2): (0.05-0.1): (0.5-1): (2.5-3), the composition is more effective in reducing sebum secretion and unblocking sebaceous glands.

[0073] Test Example 2: Antibacterial Rate Test

[0074] Acne in menstruating women is mainly related to fluctuations in hormone levels, especially the decrease in estrogen. This hormonal change leads to increased secretion of sebaceous glands, and excess sebum provides good conditions for the growth of Propionibacterium acnes. In addition, these bacteria can use the oil in the skin to grow and reproduce, and produce a variety of inflammatory factors, triggering local inflammatory reactions, leading to aggravated acne symptoms.

[0075] The compositions of Examples 1-7 and the compositions of Comparative Examples 1-5 were tested according to the following steps. The results are shown in Table 2.

[0076] Experimental method: Prepare liquid thioglycollate medium (FT medium), inoculate Propionibacterium acnes into the medium, and culture at 37°C for 48 hours to form a bacterial solution. Each composition is prepared with DMSO to form a sample solution with a volume ratio of 1% for standby use, then 180 μL of the bacterial solution is added to a 96-well plate, and then 40 μL of each sample solution is added to make a sample group, and a control group (bacterial solution without sample solution) is set up respectively. After culturing for 24 hours, the OD value of each well is measured with an ELISA instrument. The smaller the OD value, the clearer the liquid, the worse the activity of the bacteria, and the better the antibacterial effect of the composition. Then the antibacterial rate is calculated according to the following formula: Antibacterial rate (%) = (control group OD value - sample group OD value) / control group OD value × 100%; the antibacterial rate data is shown in Table 2.

[0077] Table 2 Inhibition rate of each sample solution on Propionibacterium acnes

[0078] Group Inhibition rate of Propionibacterium acnes / % Example 1 99.5 Example 2 98.0 Example 3 97.8 Example 4 97.2 Example 5 96.6 Example 6 90.6 Example 7 89.4 Comparative Example 1 31.0 Comparative Example 2 31.4 Comparative Example 3 32.6 Comparative Example 4 32.9 Comparative Example 5 31.8

[0079] From the data of Example 1 and Comparative Examples 1-5 in Table 2, it can be seen that the inhibition rate of the composition of Example 1, which is prepared by using five ingredients including mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract, on Propionibacterium acnes is significantly improved compared with the compositions of Comparative Examples 1-5, indicating that the five ingredients including mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract can synergistically inhibit Propionibacterium acnes, thereby preventing the occurrence of acne and improving existing acne.

[0080] Combined with the data of Examples 1-7, it can be seen that when the weight ratio of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract is (1.5-2.5): (1.5-2): (0.05-0.1): (0.5-1): (2.5-3), the composition has a better inhibitory effect on Propionibacterium acnes.

[0081] Test Example 3: Test of 5-α-reductase inhibition rate

[0082] 5-α reductase is an enzyme that promotes the conversion of testosterone into dihydrotestosterone (DHT), and DHT is closely related to the overactivity of sebaceous glands. During menstruation, the estrogen level in women decreases, and androgens increase relatively, leading to increased secretion of sebaceous glands, thereby causing the occurrence of acne. This change in hormone levels makes the skin more susceptible to bacteria such as Propionibacterium acnes, causing hair follicle blockage and inflammatory response. Therefore, inhibiting the activity of 5-α reductase can effectively reduce the generation of DHT, thereby reducing sebum secretion and inhibiting inflammatory response. The inhibitory activity of the detection composition on 5-α reductase can not only regulate the secretion state of sebaceous glands, but also reduce the greasy feeling of the skin and the generation of acne. In addition, reducing the production of DHT can also reduce acne-related inflammation, promote skin healing and improve overall skin quality. Therefore, for menstrual women, inhibiting 5-α reductase to improve the treatment of acne is of great significance.

[0083] The compositions of Examples 1-7 and the compositions of Comparative Examples 1-5 were tested according to the following steps. The results are shown in Table 3.

[0084] Experimental method: Each composition was prepared with DMSO into a sample solution with a volume ratio of 10%.

[0085] (1) Sample tube: Add 1 mL of each group of sample solution of the examples and comparative examples, 1 mL of enzyme solution, 1 mL of NADPH solution and 1 mL of testosterone solution into a test tube, shake gently to obtain a mixed liquid, take 200 μL of the mixed liquid into a 96-well ELISA plate with a pipette, make three replicates for each sample, put it into an ELISA reader for detection, and measure the absorbance at 340 nm, which is the first measurement value A. 样品0 ; After the sample is incubated at 37°C for 20 minutes, it is placed in an ELISA instrument for detection and the absorbance value is measured at 340nm, which is the second measurement value A 样品1 The enzyme solution is 50U of 5-α reductase diluted with pH 6.8 PBS buffer; the NADPH solution is a 2mM solution of NADPH prepared with pH 6.8 PBS; the testosterone solution is a 0.83nM solution of testosterone prepared with a 75% mass concentration ethanol solution.

[0086] (2) Enzyme tube: Add 1 mL PBS solution, 1 mL enzyme solution, 1 mL NADPH solution and 1 mL testosterone solution into a test tube, shake gently to obtain a mixed liquid, use a pipette to take 200 uL of the mixed liquid into a 96-well ELISA plate, make three replicates for each sample, place it into an ELISA reader for detection, and measure the absorbance at 340 nm, which is the first measured value A. 酶0 ; After the sample is incubated at 37°C for 20 minutes, it is placed in an ELISA instrument for detection and the absorbance value is measured at 340nm, which is the second measurement value A 酶1 ; Blank and positive control tests were performed simultaneously, wherein the blank control group was prepared by replacing the sample solution in the sample tube with an equal amount of deionized water, and the positive control group was prepared by replacing 1 mL of a 25 g / L dutasteride solution with DMSO as the solvent, and the steps were the same as above.

[0087] 5-α reductase inhibition rate (%) = [1-(A 样品0 -A 样品1 ) / (A 酶0 -A 酶1 )]×100%

[0088] Table 3 Data on the inhibition rate of 5-α reductase in each group of samples

[0089]

[0090]

[0091] From the data of Example 1 and Comparative Examples 1-5 in Table 3, it can be seen that the inhibition rate of 5-α reductase in the composition of Example 1, which is prepared by selecting five ingredients including mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract, is significantly improved compared with the compositions of Comparative Examples 1-5, indicating that the five ingredients including mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract can synergistically inhibit 5-α reductase to reduce the stimulation of androgen, thereby reducing the increase in sebum secretion due to menstrual hormone fluctuations.

[0092] Combined with the data of Examples 1-7, it can be seen that when the weight ratio of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract is (1.5-2.5): (1.5-2): (0.05-0.1): (0.5-1): (2.5-3), the composition has a better inhibitory effect on 5-α reductase.

[0093] Application Example 1-7 and Comparative Application Example 1-5

[0094] The compositions of Examples 1-7 and Comparative Examples 1-5 were added to the essence at a concentration of 5 wt % to obtain the essences of Application Examples 1-7 and Comparative Application Examples 1-5. The formulas are shown in Table 4.

[0095] The preparation method of the essence of Application Examples 1-7 and Comparative Application Examples 1-5 comprises the following steps:

[0096] The preparation method of the essence comprises the following steps:

[0097] S1. Mix the moisturizer, thickener and 1 / 2 amount of deionized water, homogenize at 80°C, cool to 60°C, add the preservative, stir evenly, and obtain a mixture;

[0098] S2. When the temperature drops to 40° C., add the components of the composition and the remaining deionized water to the mixture obtained in step S1 and stir evenly, and finally add a pH adjuster to adjust the pH to 6 to obtain the essence.

[0099] Table 4 Cream formulas of application examples 1-7 and comparative application examples 1-5

[0100]

[0101]

[0102] Comparative Application Example 6

[0103] In comparative application example 6, the essence liquid does not contain the composition, and an equal amount of deionized water is used to replace the composition. The preparation method is the same as that of application example 1.

[0104] Test Example 4

[0105] Essence efficacy test

[0106] The specific test method is as follows: 65 female subjects who are prone to acne during menstruation were screened by filling out questionnaires and randomly divided into 13 groups, 5 people in each group. The subjects had obvious acne, blackheads, and papules. The subjects continued to use the product on their entire face for 28 days, including their menstrual period; the subjects applied 1g of the essence of application examples 1-7 and comparative application examples 1-6 to their faces every morning and evening after cleansing and moisturizing, and massaged to absorb. The acne area can be overlapped and applied to the entire face once in the morning and evening, and a follow-up visit was conducted after 28 days.

[0107] (1) Objective numerical test: The subjects were tested on day 0 and 28. On the day of the visit, the subjects washed their faces with water without applying any products and sat in an air-conditioned room at a temperature of 21±1°C and a humidity of 50±10% for 20 minutes. The improvement of facial acne indicators was quantified and photographed using instruments, including acne counting (calculating the total number of comedones and papules, using VISIA and doctor evaluation), and the MX 18 probe to measure the color of facial erythema.

[0108] The test evaluates the acne removal and soothing effects of the essence on the subjects through the improvement rate of acne number and facial erythema. The calculation method is: the improvement rate of objective values ​​before and after use = |mean value before use - mean value after use| / mean value before use × 100%; see Table 5 for data.

[0109] (2) Subjective numerical test: The subjects' improvement in facial acne and anxiety was tested by a self-assessment questionnaire, which was filled out on day 28. The questionnaire was as follows:

[0110] Questionnaire 1: Improvement of facial acne during menstruation by using serum;

[0111] No improvement: 0 points, slight improvement: 1 point, general improvement: 2 points, effective improvement: 3 points, great improvement: 4 points

[0112] Questionnaire 2: Relief of anxiety after using the serum;

[0113] No relief: 0 points, slight relief: 1 point, general relief: 2 points, effective relief: 3 points, great relief: 4 points The improvement scores of menstrual acne and anxiety of each subject after 28 days were recorded, as shown in Table 4. The average score of each group represents the improvement effect of the essence on self-perceived acne and anxiety.

[0114] Table 5 Efficacy test data of each group of samples

[0115] Group Acne improvement rate / % Erythema improvement rate / % Acne improvement score Anxiety improvement score Application Example 1 99.6 98.9 3.4 3.6 Application Example 2 89.8 87.3 2.6 2.4 Application Example 3 93.3 94.4 2.4 2.8 Application Example 4 94.2 95.8 2.4 2.4 Application Example 5 97.2 96.1 2.6 2.4 Application Example 6 97.7 96.8 1.8 1.6 Application Example 7 98.3 97.6 1.8 1.4 Comparative application example 1 31.3 30.9 1.0 0.8 Comparative Application Example 2 31.3 32.0 0.8 1.0 Comparative Application Example 3 30.2 30.2 0.6 0.8 Comparative Application Example 4 30.9 31.3 0.8 1.0 Comparative Application Example 5 28.8 31.4 1.0 0.6 Comparative Application Example 6 2.3 1.2 0.4 0.2

[0116] As can be seen from Table 5, the essence of the present invention contains a composition composed of five ingredients: mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract. These five ingredients have a synergistic effect, so that the essence has better acne removal and soothing effects.

[0117] Depend on Figure 1 It can be seen that after the subject used the essence of Application Example 1 for 28 days, the red area of ​​the VISA graph of the skin test was significantly reduced, indicating that the essence can significantly reduce skin inflammation and significantly improve the acne problem. In contrast, the essence of Application Example 6 does not add the composition, so its anti-inflammatory effect is not obvious, indicating that the application of the composition of the present invention to cosmetics can improve facial acne in female acne subjects during menstruation.

[0118] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A composition containing guaiac extract for improving acne in women during menstruation, characterized in that: The invention is composed of the following components: mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract, wherein the weight ratio of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract is (0.1-5): (0.1-5): (0.01-1): (0.1-3): (0.1-3).

2. The composition for improving acne in women during menstruation containing the guaiac extract according to claim 1, characterized in that: The weight ratio of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is (1-3): (1-2): (0.01-0.5): (0.5-3): (1-3).

3. The composition for improving acne in women during menstruation containing the guaiac extract according to claim 1, characterized in that: The weight ratio of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is (1.5-2.5): (1.5-2): (0.05-0.1): (0.5-1): (2.5-3).

4. Use of the composition for improving acne in women during menstruation containing the guaiac extract according to any one of claims 1 to 3 in the preparation of skin care products.

5. Use of a composition for improving acne in women during menstruation containing a guaiac extract as claimed in claim 4 in the preparation of skin care products, characterized in that: The skin care product is at least one of lotion, emulsion, cream, mask, essence or spray, and the added amount of the composition is 0.5%-5% of the total weight of the skin care product.

6. An essence, characterized in that: The invention comprises the following raw materials in weight percentage: 0.5%-5% of the composition for improving acne in women during menstruation containing the guaiac extract according to any one of claims 1 to 3, 0.05%-0.3% of a thickener, 0.5%-5% of a moisturizer, 0.5%-3% of a preservative, 0.01%-0.3% of a pH regulator and 0.01%-1% of an essence, and the balance is deionized water.

7. The essence according to claim 6, characterized in that The raw material is selected from at least one of (a) to (d): (a) the thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol; (b) the moisturizing agent comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, hydrolyzed sodium hyaluronate, 1,2-butylene glycol, glycerol, budding pullulan polysaccharide, and ceramide; (c) the pH adjusting agent comprises at least one of arginine, tromethamine, and EDTA-disodium; (d) The preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone.

8. The method for preparing the essence according to claim 7, characterized in that: The following steps are involved: S1. Mix the moisturizer, thickener and part of deionized water, homogenize at 75-85°C, cool to 55-65°C, add the preservative, stir evenly, and obtain a mixture; S2. When the temperature drops to 35-45° C., add the components in the composition and the remaining deionized water to the mixture obtained in step S1, stir evenly, and finally add a pH adjuster to adjust the pH to obtain the essence.

Images