Essential oil-containing traditional Chinese medicine whitening and freckle-removing cream and preparation method thereof

Abstract

According to the innovative essential oil-containing traditional Chinese medicine whitening and freckle-removing cream provided by the invention, various traditional Chinese medicine extracts with whitening and freckle-removing effects are carefully selected as main active ingredients, and are extracted and concentrated by a scientific water distillation method, so that effective utilization of traditional Chinese medicine ingredients is ensured. Meanwhile, essential oil, base oil, an emulsifier, a humectant, a natural substance additive (such as pearl powder, oat beta-glucan and the like) and an antibacterial additive (such as chitosan) in a specific proportion are combined to jointly form a unique formula of the freckle-removing cream. The freckle-removing cream disclosed by the invention not only has remarkable whitening and freckle-removing effects, but also has good skin-care efficacy and comfort. The preparation method is simple and efficient, and a uniform and stable product can be prepared through conventional heating, stirring and homogenizing treatment. The freckle-removing cream is suitable for preparing various freckle-removing medicines or cosmetics, and provides a safe, effective and comfortable choice for consumers pursuing skin whitening and freckle removal.

Description

Technical Field

[0001] The invention relates to the field of Chinese medicine extracts, and specifically discloses a Chinese medicine whitening and anti-freckle cream containing essential oil and a preparation method thereof. Background Art

[0002] In the field of beauty and skin care, whitening and freckle removal has always been an eternal theme pursued by consumers. With the continuous improvement of people's requirements for skin health and beauty effects, traditional whitening and freckle removal products have been unable to meet the diversified and personalized needs of the market. Whitening and freckle removal products in the existing technology have many shortcomings and are in urgent need of improvement and innovation.

[0003] First, from the perspective of chemical composition, traditional whitening and freckle removal products mostly rely on chemical synthetic ingredients, such as hydroquinone, arbutin, etc. Although these ingredients can achieve the effect of whitening and freckle removal to a certain extent, long-term use may have serious side effects on human skin. For example, hydroquinone may cause skin pigmentation, allergies and even cancer, while arbutin may cause adverse reactions such as skin redness, swelling, and stinging. The use of these chemical ingredients not only affects the safety of the product, but also limits its market application prospects.

[0004] Secondly, in terms of the application of Chinese herbal ingredients, traditional whitening and freckle removal products also have many problems. On the one hand, the extraction methods of Chinese herbal ingredients are often relatively simple and rough, and it is difficult to ensure the effectiveness and stability of Chinese herbal ingredients. Although many products claim to contain Chinese herbal ingredients, in fact, the content of Chinese herbal ingredients is low, and there may be problems with the loss or destruction of effective ingredients during the extraction process. On the other hand, there is also a lack of scientific and rigorous research on the compatibility and proportion of Chinese herbal ingredients. The interaction mechanisms between different Chinese herbal ingredients are complex and diverse, and unreasonable compatibility and proportion may lead to the inability to fully exert the synergistic effect of Chinese herbal ingredients, thereby affecting the overall effect of the product.

[0005] In addition, traditional whitening and anti-freckle products also have problems such as single texture and poor feeling of use. Many products are too greasy or dry, difficult to be fully absorbed by the skin, resulting in poor effect. At the same time, some products may also contain irritating ingredients such as alcohol and fragrances, which put an extra burden on the skin. Summary of the invention

[0006] In view of the above problems, the present invention provides a Chinese medicine whitening and freckle-removing cream containing essential oils and a preparation method thereof. The present invention, through in-depth research on the whitening and freckle-removing mechanism of Chinese medicine ingredients, carefully screens a variety of Chinese medicines with significant whitening and freckle-removing effects, and uses a scientific water distillation method to extract Chinese medicine ingredients, thereby ensuring the effectiveness and stability of the Chinese medicine ingredients. At the same time, the present invention also combines essential oils, base oils, emulsifiers, moisturizers, natural material additives and antibacterial additives in a specific proportion to form a unique formula of this freckle-removing cream. This formula not only has a significant whitening and freckle-removing effect, but also can nourish the skin, improve skin quality, and has a refreshing texture and is easy to absorb.

[0007] The technical solution of the present invention is as follows: A traditional Chinese medicine whitening and anti-freckle cream containing essential oil, the whitening and anti-freckle cream is made of the following raw materials in parts by weight: Chinese herbal medicine extract: extracted from one or more of astragalus, codonopsis, rhodiola, kudzu root, coix seed, and angelica, with a total weight of 5-30 parts; Essential oil: at least one selected from lavender essential oil, rose essential oil, lemon essential oil, and tea tree essential oil, in 4-8 parts by weight; Base oil: one or more selected from olive oil, sweet almond oil, grape seed oil, 20-50 parts by weight; Emulsifier: 4-8 parts by weight; Moisturizer: 2-10 parts by weight; Natural material additives: one or more selected from pearl powder, honey, oat β-glucan, seaweed extract, with a total weight of 5-10 parts; Antibacterial additive: 3-6 parts by weight of chitosan; Deionized water: 5-10 parts by weight.

[0008] Furthermore, in the above-mentioned traditional Chinese medicine whitening and freckle-removing cream containing essential oils, the traditional Chinese medicine extract is extracted from astragalus, codonopsis, rhodiola, kudzu root, coix seed and angelica by water distillation, and the weight ratio of the raw materials of each traditional Chinese medicine is astragalus: codonopsis: rhodiola: kudzu root: coix seed: angelica = 2:1:1:1:2:1.

[0009] Furthermore, in the above-mentioned traditional Chinese medicine whitening and anti-freckle cream containing essential oil, the traditional Chinese medicine extract is prepared by the following steps: (1) Raw material preparation: Select fresh medicinal parts of astragalus, codonopsis, rhodiola, kudzu root, coix seed and angelica as raw materials, and weigh them according to the weight ratio of astragalus: codonopsis: rhodiola: kudzu root: coix seed: angelica = 2:1:1:1:2:1; (2) Water distillation extraction: Place the Chinese medicinal materials in step 1) into a distillation pot, add an appropriate amount of clean water, and ensure that the water surface covers the Chinese medicinal materials. Then heat the distillation pot to boil the water and generate steam. The volatile components in the Chinese medicinal materials are evaporated along with the steam. The evaporated steam is condensed into liquid through a condenser and collected to obtain the primary Chinese medicinal extract. (3) Filtration and concentration: Filter the Chinese herbal medicine extract obtained in step (2) through filter paper or a filter to remove impurities and suspended matter therein, and then concentrate the filtered extract by heating to evaporate part of the water so that the concentration of the extract reaches 1.05-1.1 g / mL; (4) Vacuum drying: The extract obtained in step (3) is dried in a vacuum at a temperature not exceeding 80°C to obtain a powdered extract.

[0010] Furthermore, in the above-mentioned traditional Chinese medicine whitening and anti-freckle cream containing essential oil, the essential oil is a mixture of lavender essential oil, rose essential oil and tea tree essential oil, and the weight ratio of lavender essential oil to rose essential oil and tea tree essential oil is 3:1:1.

[0011] Furthermore, in the above-mentioned traditional Chinese medicine whitening and anti-freckle cream containing essential oils, the base oil is a mixture of olive oil and sweet almond oil, and the weight ratio of olive oil to sweet almond oil is 2:1.

[0012] Furthermore, in the above-mentioned traditional Chinese medicine whitening and anti-freckle cream containing essential oil, the emulsifier is Tween 80, the moisturizer is a mixture of glycerol and propylene glycol, and the weight ratio of glycerol to propylene glycol is 1:1.

[0013] Furthermore, in the above-mentioned traditional Chinese medicine whitening and anti-freckle cream containing essential oil, the natural substance additive is a mixture of pearl powder and oat β-glucan, and the weight ratio of pearl powder to oat β-glucan is 2:1.

[0014] Furthermore, in the above-mentioned traditional Chinese medicine whitening and anti-freckle cream containing essential oil, the antibacterial additive is chitosan with a deacetylation degree of 85%.

[0015] The present invention also discloses a preparation method of the above-mentioned traditional Chinese medicine whitening and anti-freckle cream containing essential oil, comprising the following steps: adding traditional Chinese medicine extract, essential oil, base oil, emulsifier, moisturizer, natural material additive, antibacterial additive and deionized water into a mixing container in sequence, heating to 45-55°C, stirring for 20 minutes, and homogenizing at a pressure of 20-30MPa to obtain a uniform and stable traditional Chinese medicine whitening and anti-freckle cream.

[0016] The invention also discloses application of the traditional Chinese medicine whitening and freckle-removing cream containing essential oil in preparing freckle-removing medicines or cosmetics.

[0017] Compared with the prior art, the present invention has the following beneficial effects: 1. Safe and effective: The present invention uses natural Chinese herbal extracts as the main active ingredients, avoiding the side effects that may be caused by chemically synthesized ingredients in traditional whitening and freckle removal products. The Chinese herbal extracts are extracted and concentrated by scientific water distillation to ensure their effectiveness and stability, thereby achieving a safe and effective whitening and freckle removal effect.

[0018] 2 Unique formula: The invention combines essential oils, base oils, emulsifiers, moisturizers, natural additives and antibacterial additives in a specific proportion to form a unique formula for this anti-freckle cream. This formula not only has a significant whitening and freckle removal effect, but also nourishes the skin and improves skin quality, providing consumers with a full range of skin beauty experiences.

[0019] 3. Non-irritating: The present invention does not contain irritating ingredients such as alcohol and irritating fragrances, is mild and non-irritating to the skin, and is suitable for people of all skin types. Especially for consumers with sensitive skin, the freckle cream of the present invention is undoubtedly a safer choice.

[0020] 4. Simple preparation process: The preparation method of the present invention is simple and efficient, and a uniform and stable product can be obtained by conventional heating, stirring and homogenization. This not only reduces the production cost, but also improves the production efficiency, making the freckle cream of the present invention easier to promote and apply. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Comparison of free radical scavenging rate results (%) of Examples 1-3 and Comparative Examples 1-4; Figure 2 Comparison of tyrosinase inhibition rate (%) between Example 2 and Comparative Examples 1-4. DETAILED DESCRIPTION

[0022] A traditional Chinese medicine whitening and anti-freckle cream containing essential oil, the whitening and anti-freckle cream is made of the following raw materials in parts by weight: Chinese herbal medicine extract: 5-30 parts by weight; Essential oil: 4-8 parts by weight; Base oil: 20-50 parts by weight; Emulsifier: 4-8 parts by weight; Moisturizer: 2-10 parts by weight; Natural material additives: 5-10 parts by weight; Antibacterial additive: 3-6 parts by weight of chitosan; Deionized water: 5-10 parts by weight.

[0023] Preferably, the Chinese medicine extract is extracted from astragalus, codonopsis, rhodiola, kudzu root, coix seed and angelica by water distillation, and the weight ratio of the raw materials of each Chinese medicinal material is astragalus: codonopsis: rhodiola: kudzu root: coix seed: angelica = 2:1:1:1:2:1.

[0024] Preferably, the Chinese medicine extract is prepared by the following steps: (1) Raw material preparation: Select fresh medicinal parts of astragalus, codonopsis, rhodiola, kudzu root, coix seed and angelica as raw materials, and weigh them according to the weight ratio of astragalus: codonopsis: rhodiola: kudzu root: coix seed: angelica = 2:1:1:1:2:1; (2) Water distillation extraction: Place the Chinese medicinal materials in step 1) into a distillation pot, add an appropriate amount of clean water, and ensure that the water surface covers the Chinese medicinal materials. Then heat the distillation pot to boil the water and generate steam. The volatile components in the Chinese medicinal materials are evaporated along with the steam. The evaporated steam is condensed into liquid through a condenser and collected to obtain the primary Chinese medicinal extract. (3) Filtration and concentration: Filter the Chinese herbal medicine extract obtained in step (2) through filter paper or a filter to remove impurities and suspended matter therein, and then concentrate the filtered extract by heating to evaporate part of the water so that the concentration of the extract reaches 1.05-1.1 g / mL; (4) Vacuum drying: The extract obtained in step (3) is dried in a vacuum at a temperature not exceeding 80°C to obtain a powdered extract.

[0025] Preferably, the essential oil is a mixture of lavender essential oil, rose essential oil and tea tree essential oil, and the weight ratio of lavender essential oil to rose essential oil and tea tree essential oil is 3:1:1.

[0026] Preferably, the base oil is a mixture of olive oil and sweet almond oil, and the weight ratio of olive oil to sweet almond oil is 2:1.

[0027] Preferably, the emulsifier is Tween 80, and the humectant is a mixture of glycerol and propylene glycol, and the weight ratio of glycerol to propylene glycol is 1:1.

[0028] Preferably, the natural substance additive is a mixture of pearl powder and oat β-glucan, and the weight ratio of pearl powder to oat β-glucan is 2:1.

[0029] Preferably, the antibacterial additive is chitosan with a degree of deacetylation of 85%.

[0030] The preparation method of the above-mentioned traditional Chinese medicine whitening and anti-freckle cream containing essential oils comprises the following steps: adding traditional Chinese medicine extracts, essential oils, base oils, emulsifiers, moisturizers, natural material additives, antibacterial additives and deionized water into a mixing container in sequence, heating to 45-55°C, stirring for 20 minutes, and homogenizing at a pressure of 20-30MPa to obtain a uniform and stable traditional Chinese medicine whitening and anti-freckle cream.

[0031] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Example 1 A traditional Chinese medicine whitening and anti-freckle cream containing essential oil, the whitening and anti-freckle cream is made of the following raw materials in parts by weight: Chinese herbal medicine extract: total weight parts: 5 parts; Essential oil: 4 parts by weight; Base oil: 20 parts by weight; Emulsifier: 4 parts by weight; Moisturizer: 2 parts by weight; Natural material additives: total weight parts: 5 parts; Antibacterial additive: 3-6 parts by weight of chitosan; Deionized water: 5-10 parts by weight.

[0033] The Chinese medicine extract is extracted from astragalus, codonopsis, rhodiola, kudzu root, coix seed and angelica by water distillation, and the weight ratio of the raw materials of each Chinese medicinal material is astragalus: codonopsis: rhodiola: kudzu root: coix seed: angelica = 2:1:1:1:2:1.

[0034] The Chinese medicine extract is prepared by the following steps: (1) Raw material preparation: Select fresh medicinal parts of astragalus, codonopsis, rhodiola, kudzu root, coix seed and angelica as raw materials, and weigh them according to the weight ratio of astragalus: codonopsis: rhodiola: kudzu root: coix seed: angelica = 2:1:1:1:2:1; (2) Water distillation extraction: Place the Chinese medicinal materials in step 1) into a distillation pot, add an appropriate amount of clean water, and ensure that the water surface covers the Chinese medicinal materials. Then heat the distillation pot to boil the water and generate steam. The volatile components in the Chinese medicinal materials are evaporated along with the steam. The evaporated steam is condensed into liquid through a condenser and collected to obtain the primary Chinese medicinal extract. (3) Filtration and concentration: Filter the Chinese herbal medicine extract obtained in step (2) through filter paper or a filter to remove impurities and suspended matter therein, and then concentrate the filtered extract by heating to evaporate part of the water so that the concentration of the extract reaches 1.05-1.1 g / mL; (4) Vacuum drying: The extract obtained in step (3) is dried in a vacuum at a temperature not exceeding 80°C to obtain a powdered extract.

[0035] The essential oil is a mixture of lavender essential oil, rose essential oil and tea tree essential oil, and the weight ratio of lavender essential oil to rose essential oil and tea tree essential oil is 3:1:1.

[0036] The base oil is a mixture of olive oil and sweet almond oil, and the weight ratio of olive oil to sweet almond oil is 2:1.

[0037] The emulsifier is Tween 80, and the moisturizer is a mixture of glycerol and propylene glycol, and the weight ratio of glycerol to propylene glycol is 1:1.

[0038] The natural material additive is a mixture of pearl powder and oat beta-glucan, and the weight ratio of the pearl powder to the oat beta-glucan is 2:1.

[0039] The antibacterial additive is chitosan with a deacetylation degree of 85%.

[0040] The preparation method of the above-mentioned traditional Chinese medicine whitening and anti-freckle cream containing essential oils comprises the following steps: adding traditional Chinese medicine extracts, essential oils, base oils, emulsifiers, moisturizers, natural material additives, antibacterial additives and deionized water into a mixing container in sequence, heating to 45-55°C, stirring for 20 minutes, and homogenizing at a pressure of 20-30MPa to obtain a uniform and stable traditional Chinese medicine whitening and anti-freckle cream.

[0041] Example 2 A traditional Chinese medicine whitening and anti-freckle cream containing essential oil, the whitening and anti-freckle cream is made of the following raw materials in parts by weight: Chinese herbal medicine extract: total weight parts is 15 parts; Essential oil: 6 parts by weight; Base oil: 35 parts by weight; Emulsifier: 6 parts by weight; Moisturizer: 5 parts by weight; Natural material additives: total weight parts: 8 parts; Antibacterial additive: 5 parts by weight of chitosan; Deionized water: 8 parts by weight.

[0042] The Chinese medicine extract is extracted from astragalus, codonopsis, rhodiola, kudzu root, coix seed and angelica by water distillation, and the weight ratio of the raw materials of each Chinese medicinal material is astragalus: codonopsis: rhodiola: kudzu root: coix seed: angelica = 2:1:1:1:2:1.

[0043] The Chinese medicine extract is prepared by the following steps: (1) Raw material preparation: Select fresh medicinal parts of astragalus, codonopsis, rhodiola, kudzu root, coix seed and angelica as raw materials, and weigh them according to the weight ratio of astragalus: codonopsis: rhodiola: kudzu root: coix seed: angelica = 2:1:1:1:2:1; (2) Water distillation extraction: Place the Chinese medicinal materials in step 1) into a distillation pot, add an appropriate amount of clean water, and ensure that the water surface covers the Chinese medicinal materials. Then heat the distillation pot to boil the water and generate steam. The volatile components in the Chinese medicinal materials are evaporated along with the steam. The evaporated steam is condensed into liquid through a condenser and collected to obtain the primary Chinese medicinal extract. (3) Filtration and concentration: Filter the Chinese herbal medicine extract obtained in step (2) through filter paper or a filter to remove impurities and suspended matter therein, and then concentrate the filtered extract by heating to evaporate part of the water so that the concentration of the extract reaches 1.05-1.1 g / mL; (4) Vacuum drying: The extract obtained in step (3) is dried in a vacuum at a temperature not exceeding 80°C to obtain a powdered extract.

[0044] The essential oil is a mixture of lavender essential oil, rose essential oil and tea tree essential oil, and the weight ratio of lavender essential oil to rose essential oil and tea tree essential oil is 3:1:1.

[0045] The base oil is a mixture of olive oil and sweet almond oil, and the weight ratio of olive oil to sweet almond oil is 2:1.

[0046] The emulsifier is Tween 80, and the moisturizer is a mixture of glycerol and propylene glycol, and the weight ratio of glycerol to propylene glycol is 1:1.

[0047] The natural material additive is a mixture of pearl powder and oat beta-glucan, and the weight ratio of the pearl powder to the oat beta-glucan is 2:1.

[0048] The antibacterial additive is chitosan with a deacetylation degree of 85%.

[0049] The preparation method of the above-mentioned traditional Chinese medicine whitening and anti-freckle cream containing essential oils comprises the following steps: adding traditional Chinese medicine extracts, essential oils, base oils, emulsifiers, moisturizers, natural material additives, antibacterial additives and deionized water into a mixing container in sequence, heating to 45-55°C, stirring for 20 minutes, and homogenizing at a pressure of 20-30MPa to obtain a uniform and stable traditional Chinese medicine whitening and anti-freckle cream.

[0050] Example 3 A traditional Chinese medicine whitening and anti-freckle cream containing essential oil, the whitening and anti-freckle cream is made of the following raw materials in parts by weight: Chinese herbal medicine extract: total weight parts: 30 parts; Essential oil: 8 parts by weight; Base oil: 50 parts by weight; Emulsifier: 8 parts by weight; Moisturizing agent: 10 parts by weight; Natural material additives: total weight parts: 10 parts; Antibacterial additive: 6 parts by weight of chitosan; Deionized water: 10 parts by weight.

[0051] The Chinese medicine extract is extracted from astragalus, codonopsis, rhodiola, kudzu root, coix seed and angelica by water distillation, and the weight ratio of the raw materials of each Chinese medicinal material is astragalus: codonopsis: rhodiola: kudzu root: coix seed: angelica = 2:1:1:1:2:1.

[0052] The Chinese medicine extract is prepared by the following steps: (1) Raw material preparation: Select fresh medicinal parts of astragalus, codonopsis, rhodiola, kudzu root, coix seed and angelica as raw materials, and weigh them according to the weight ratio of astragalus: codonopsis: rhodiola: kudzu root: coix seed: angelica = 2:1:1:1:2:1; (2) Water distillation extraction: Place the Chinese medicinal materials in step 1) into a distillation pot, add an appropriate amount of clean water, and ensure that the water surface covers the Chinese medicinal materials. Then heat the distillation pot to boil the water and generate steam. The volatile components in the Chinese medicinal materials are evaporated along with the steam. The evaporated steam is condensed into liquid through a condenser and collected to obtain the primary Chinese medicinal extract. (3) Filtration and concentration: Filter the Chinese herbal medicine extract obtained in step (2) through filter paper or a filter to remove impurities and suspended matter therein, and then concentrate the filtered extract by heating to evaporate part of the water so that the concentration of the extract reaches 1.05-1.1 g / mL; (4) Vacuum drying: The extract obtained in step (3) is dried in a vacuum at a temperature not exceeding 80°C to obtain a powdered extract.

[0053] The essential oil is a mixture of lavender essential oil, rose essential oil and tea tree essential oil, and the weight ratio of lavender essential oil to rose essential oil and tea tree essential oil is 3:1:1.

[0054] The base oil is a mixture of olive oil and sweet almond oil, and the weight ratio of olive oil to sweet almond oil is 2:1.

[0055] The emulsifier is Tween 80, and the moisturizer is a mixture of glycerol and propylene glycol, and the weight ratio of glycerol to propylene glycol is 1:1.

[0056] The natural material additive is a mixture of pearl powder and oat beta-glucan, and the weight ratio of the pearl powder to the oat beta-glucan is 2:1.

[0057] The antibacterial additive is chitosan with a deacetylation degree of 85%.

[0058] The preparation method of the above-mentioned traditional Chinese medicine whitening and anti-freckle cream containing essential oils comprises the following steps: adding traditional Chinese medicine extracts, essential oils, base oils, emulsifiers, moisturizers, natural material additives, antibacterial additives and deionized water into a mixing container in sequence, heating to 45-55°C, stirring for 20 minutes, and homogenizing at a pressure of 20-30MPa to obtain a uniform and stable traditional Chinese medicine whitening and anti-freckle cream.

[0059] Comparative Example 1 Changing the composition of Chinese herbal medicine extracts Changes: Reduce the raw materials of Chinese herbal medicine extract from 6 to 4, namely, Astragalus, Codonopsis: Rhodiola: Pueraria = 2:1:1:1 Comparative Example 2 Changing the proportion of ingredients in Chinese herbal medicine extracts Changes: The raw material ratio of traditional Chinese medicine extracts is changed from Astragalus: Codonopsis: Rhodiola: Pueraria: Coix seed: Angelica = 2:1:1:1:2:1 to Astragalus: Codonopsis: Rhodiola: Pueraria: Coix seed: Angelica = 1:1:1:1:1:1:1.

[0060] Comparative Example 3 Changing the type of essential oil Changes: The mixture of lavender essential oil, rose essential oil and tea tree essential oil (weight ratio of 3:1:1) was replaced with single lavender essential oil.

[0061] Comparative Example 4 Changes in natural substance additives Changes: Only pearl powder is used as a natural additive, and oat beta-glucan is not used.

[0062] Test Example 1 Antioxidant stability test Objective: To determine the scavenging rate of each solution on active free radicals by using the Fe²⁺ oxidation method, so as to evaluate its antioxidant stability.

[0063] Experimental groups: Experimental group: Examples 1-3, Comparative Examples 1-4.

[0064] Control group: normal saline (negative control), butylated hydroxytoluene BHT (positive control).

[0065] Sample preparation: To facilitate the experiment, each experimental group of samples was prepared into a 5% aqueous solution.

[0066] Experimental steps: 1. Place the solution to be tested into a sealed bottle and place it in a constant temperature box at (60±2)℃ for antioxidant stability test.

[0067] 2 Samples were taken every five days, and the scavenging rate of active free radicals was determined by the Fe²⁺ oxidation method of o-phenanthroline.

[0068] Determination method: 1. Take 0.2 ml of 5×10⁻³mol / L o-phenanthroline aqueous solution and 1 ml of 50 mmol / L Tris-HCl buffer solution (pH=7.4, prepared with deionized water) in a 5 ml volumetric flask.

[0069] 2. Add 0.2 ml of 7.5×10⁻³mol / L FeSO4 aqueous solution and shake well immediately.

[0070] 3. Add 200 μL of sample solution, then add 0.2 ml of 1% H2O2, and make up to the mark with ethanol.

[0071] 4. After shaking thoroughly, place in a 37°C water bath and keep warm for 60 minutes.

[0072] 5. Use a spectrophotometer to measure its absorbance at 536 nm.

[0073] Clearance calculation: The clearance rate was calculated according to the following formula: N = [(A2 - A3) / (A1 - A3)] × 100%, where N is the clearance rate (%); A1 is the absorbance without adding H2O2 and sample; A2 is the absorbance with adding H2O2 and sample; and A3 is the absorbance with adding H2O2 but no sample.

[0074] Experimental results analysis: The scavenging rate of the solution on active free radicals was tested at 0 days, 15 days, 30 days, 60 days and 120 days to evaluate the antioxidant stability of each sample.

[0075] The results are shown in Table 1 and Figure 1 shown.

[0076] Table 1 Free radical scavenging rate results (%) From the data in Table 1, we can see that: Examples 1-3: These three examples showed high free radical scavenging rates and maintained relatively stable antioxidant properties throughout the experimental period (0d to 120d). Example 3 had the highest scavenging rate at all time points, followed by Example 2 and Example 1.

[0077] Comparative Examples 1-4: Compared with the examples, the removal rates of the comparative examples are generally lower, and the antioxidant performance decreases faster over time.

[0078] Negative control: Normal saline was used as a negative control, and its free radical scavenging rate was very low and continued to decrease over time.

[0079] Positive control: Butylated hydroxytoluene (BHT) was used as a positive control and showed a higher initial clearance rate, but its antioxidant properties also decreased over time.

[0080] Comparative Example 1 (changing the ingredients of Chinese medicine extract): The clearance rate was significantly lower than that of the embodiment, indicating that reducing the components of the Chinese herbal extract had a negative impact on the antioxidant performance.

[0081] This indicates that the lack of coix seed and angelica in the combination of astragalus, codonopsis, rhodiola rosea and kudzu root affects the overall antioxidant effect.

[0082] Comparative Example 2 (changing the proportion of Chinese medicine extract ingredients): The clearance rate is lower than that of the embodiment, indicating that the component ratio of the Chinese herbal extract has an important influence on the antioxidant performance.

[0083] The original ratio (2:1:1:1:2:1) provides the best synergy.

[0084] Comparative Example 3 (changing the type of essential oil): The removal rate is lower than that of the embodiment, but still higher than that of comparative example 1 and comparative example 4.

[0085] The results showed that a mixture of lavender, rose, and tea tree oils provided the best antioxidant effect, while lavender oil alone was second.

[0086] Comparative Example 4 (Changing natural substance additives): The decrease in clearance rate was the most obvious, indicating that oat β-glucan plays an important role in antioxidant stability.

[0087] Although pearl powder has a certain antioxidant effect, it lacks the synergistic effect of oat β-glucan, resulting in a decrease in overall antioxidant performance.

[0088] in conclusion The free radical scavenging rate of Examples 1-3 is the highest, indicating that the formula thereof is excellent in terms of antioxidant stability. Antioxidation can help reduce oxidative stress caused by ultraviolet rays, thereby indirectly promoting whitening.

[0089] The lower clearance rates of Comparative Examples 1-4 indicate that changes in the composition, ratio of components, type of essential oils or natural additives of Chinese herbal extracts will have a negative impact on the antioxidant properties. The performance of the positive control BHT verifies the reliability of the experimental method, while the low clearance rate of the negative control saline emphasizes the importance of the antioxidant components in the formula.

[0090] Test Example 2 Whitening and freckle removal effect test.

[0091] This whitening test uses the commonly used tyrosinase inhibition rate assessment method in the industry. Melanin is the main pigment produced by melanocytes in the skin, and tyrosinase is the key enzyme in the melanin production process. It catalyzes the conversion of tyrosine into DOPA, which then produces melanin through a series of complex reactions. The activity of tyrosinase directly affects the rate of melanin production. If the activity increases, melanin production increases, and if the activity is inhibited, melanin production decreases.

[0092] The experimental design is as follows: Sample group: 0.1 g of anti-freckle cream (Example 2 and Comparative Examples 1-4) was taken in each group, diluted to 1 mL with water, and then 1 mL of phosphate buffer and 1 mL of tyrosinase solution were added.

[0093] First control group: Mix 2 mL of phosphate buffer and 1 mL of tyrosinase solution.

[0094] The second control group: only 3 mL of phosphate buffer solution was taken.

[0095] The third control group: take 1 mL of each test material (i.e., a solution of 0.1 g of anti-freckle cream added with water to 1 mL) and mix with 2 mL of phosphate buffer.

[0096] The tyrosinase inhibition rate was calculated by the following formula: inhibition rate % = [(A - B) - (C - D)] / (A- B) * 100%, where A is the absorbance value of the first control group, B is the absorbance value of the second control group, C is the absorbance value of the sample group, and D is the absorbance value of the third control group.

[0097] The experimental results are recorded in Table 2 and Figure 2 middle.

[0098] Table 2 Tyrosinase inhibition rate (%) Comparative test Discussion of experimental results: Example 2: The tyrosinase inhibition rate is as high as 97.2%, showing a strong ability to inhibit tyrosinase activity.

[0099] Comparative Example 1: The tyrosinase inhibition rate was 82.3%, which was significantly lower than that of Example 2.

[0100] Comparative Example 2: The tyrosinase inhibition rate was 89.5%, which was higher than that of Comparative Example 1, but still lower than that of Example 2.

[0101] Comparative Example 3: The tyrosinase inhibition rate was 90.4%, which was closer to the level of Example 2.

[0102] Comparative Example 4: The tyrosinase inhibition rate was 87.5%, which was lower than that of Example 2 and Comparative Example 3.

[0103] Specific analysis: Comparative Example 1 (changing the ingredients of Chinese medicine extract): After reducing the raw materials of traditional Chinese medicine extract from 6 to 4, the tyrosinase inhibition rate decreased significantly.

[0104] This suggests that the reduced Chinese herbal ingredients may contain substances that have important inhibitory effects on tyrosinase, and the lack of coix seed and angelica has affected the overall tyrosinase inhibitory effect.

[0105] Comparative Example 2 (changing the proportion of Chinese medicine extract ingredients): After changing the raw material ratio of the Chinese herbal extract, the tyrosinase inhibition rate was improved, but still lower than that of Example 2.

[0106] This suggests that the original ratio (2:1:1:1:2:1) may provide the best synergistic effect, or that certain ingredients can only exert the greatest tyrosinase inhibition effect at a specific ratio.

[0107] Comparative Example 3 (changing the type of essential oil): After the mixture of lavender essential oil, rose essential oil and tea tree essential oil was replaced by single lavender essential oil, the tyrosinase inhibition rate decreased, but was still close to the level of Example 2.

[0108] This suggests that the essential oil blend may provide a better tyrosinase inhibitory effect, while the individual essential oils are less effective.

[0109] Comparative Example 4 (Changing natural substance additives): When only pearl powder was used as a natural additive without oat β-glucan, the tyrosinase inhibition rate decreased significantly. This indicates that oat β-glucan plays an important role in tyrosinase inhibition, which may be related to the synergistic effect it achieves.

[0110] in conclusion: Example 2 has excellent performance in tyrosinase inhibition rate, indicating that its formula has significant effects in inhibiting tyrosinase activity and reducing melanin production. The tyrosinase inhibition rates of Comparative Examples 1-4 are relatively low, indicating that changing the composition, composition ratio, essential oil type or natural substance additives of Chinese herbal extracts will have a negative impact on tyrosinase inhibition performance. These results demonstrate the key role of each component and its ratio in the present formula in tyrosinase inhibition, that is, the present formula has a good whitening and freckle removal effect.

[0111] The above are only a few preferred embodiments of the present invention, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A traditional Chinese medicine whitening and anti-freckle cream containing essential oils, characterized in that: The whitening and anti-freckle cream is made from the following raw materials in parts by weight: Chinese herbal medicine extract: extracted from one or more of astragalus, codonopsis, rhodiola, kudzu root, coix seed, and angelica, with a total weight of 5-30 parts; Essential oil: at least one selected from lavender essential oil, rose essential oil, lemon essential oil, and tea tree essential oil, in 4-8 parts by weight; Base oil: one or more selected from olive oil, sweet almond oil, grape seed oil, 20-50 parts by weight; Emulsifier: 4-8 parts by weight; Moisturizer: 2-10 parts by weight; Natural material additives: one or more selected from pearl powder, honey, oat β-glucan, seaweed extract, with a total weight of 5-10 parts; Antibacterial additive: 3-6 parts by weight of chitosan; Deionized water: 5-10 parts by weight.

2. The Chinese medicine whitening and anti-freckle cream containing essential oil according to claim 1, characterized in that: The Chinese medicine extract is extracted from astragalus, codonopsis, rhodiola, kudzu root, coix seed and angelica by water distillation, and the weight ratio of the raw materials of each Chinese medicinal material is astragalus: codonopsis: rhodiola: kudzu root: coix seed: angelica = 2:1:1:1:2:

1.

3. The Chinese medicine whitening and anti-freckle cream containing essential oil according to claim 1, characterized in that: The Chinese medicine extract is prepared by the following steps: (1) Raw material preparation: Select fresh medicinal parts of astragalus, codonopsis, rhodiola, kudzu root, coix seed and angelica as raw materials, and weigh them according to the weight ratio of astragalus: codonopsis: rhodiola: kudzu root: coix seed: angelica = 2:1:1:1:2:1; (2) Water distillation extraction: Place the Chinese medicinal materials in step 1) into a distillation pot, add an appropriate amount of clean water, and ensure that the water surface covers the Chinese medicinal materials. Then heat the distillation pot to boil the water and generate steam. The volatile components in the Chinese medicinal materials are evaporated along with the steam. The evaporated steam is condensed into liquid through a condenser and collected to obtain the primary Chinese medicinal extract. (3) Filtration and concentration: Filter the Chinese herbal medicine extract obtained in step (2) through filter paper or a filter to remove impurities and suspended matter therein, and then concentrate the filtered extract by heating to evaporate part of the water so that the concentration of the extract reaches 1.05-1.1 g / mL; (4) Vacuum drying: The extract obtained in step (3) is dried in a vacuum at a temperature not exceeding 80°C to obtain a powdered extract.

4. The Chinese medicine whitening and anti-freckle cream containing essential oil according to claim 1, characterized in that: The essential oil is a mixture of lavender essential oil, rose essential oil and tea tree essential oil, and the weight ratio of lavender essential oil to rose essential oil and tea tree essential oil is 3:1:

1.

5. The Chinese medicine whitening and anti-freckle cream containing essential oil according to claim 1, characterized in that: The base oil is a mixture of olive oil and sweet almond oil, and the weight ratio of olive oil to sweet almond oil is 2:

1.

6. The traditional Chinese medicine whitening and anti-freckle cream containing essential oil according to claim 1, characterized in that: The emulsifier is Tween 80, and the moisturizer is a mixture of glycerol and propylene glycol, and the weight ratio of glycerol to propylene glycol is 1:

1.

7. The traditional Chinese medicine whitening and anti-freckle cream containing essential oil according to claim 1, characterized in that: The natural material additive is a mixture of pearl powder and oat beta-glucan, and the weight ratio of the pearl powder to the oat beta-glucan is 2:

1.

8. The traditional Chinese medicine whitening and anti-freckle cream containing essential oil according to claim 1, characterized in that: The antibacterial additive is chitosan with a deacetylation degree of 85%.

9. The method for preparing the traditional Chinese medicine whitening and anti-freckle cream containing essential oil according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: adding Chinese herbal medicine extract, essential oil, base oil, emulsifier, moisturizer, natural substance additive, antibacterial additive and deionized water into a mixing container in sequence, heating to 45-55°C, stirring for 20 minutes, and homogenizing at a pressure of 20-30MPa to obtain a uniform and stable Chinese herbal medicine whitening and anti-freckle cream.

10. Use of the traditional Chinese medicine whitening and anti-freckle cream containing essential oils as claimed in any one of claims 1 to 8 in the preparation of anti-freckle medicines or cosmetics.

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