Whitening toothpaste for preventing caries and inhibiting dental plaque and preparation method thereof

Abstract

The invention belongs to the technical field of oral care products, and particularly relates to toothpaste, in particular to whitening toothpaste capable of preventing caries and inhibiting dental plaque and a preparation method of the whitening toothpaste. The toothpaste comprises the following components in parts by weight: 25-30 parts of zeolite, 3-5 parts of silicon dioxide, 1-2 parts of L-calcium aspartate, 20-30 parts of a traditional Chinese medicine extracting solution, 0.1-0.12 part of sodium benzoate, 5-8 parts of sorbitol, 5-10 parts of peach kernel enzymatic hydrolysate and 15-20 parts of water. The toothpaste prepared according to the formula has the effects of preventing caries, inhibiting dental plaque and improving periodontitis.

Description

Technical Field

[0001] The invention belongs to the technical field of oral care products, and specifically relates to a toothpaste, and in particular to a whitening toothpaste capable of preventing caries and inhibiting dental plaque, and a preparation method thereof. Background Art

[0002] Toothpaste is a daily chemical product used for oral hygiene. It is used in conjunction with a toothbrush to effectively remove dental plaque and food debris from the surface of teeth through physical friction to maintain oral health. In the current market, most toothpaste products are based on basic cleaning functions, and their purpose is to remove food debris and soft plaque in the mouth and maintain oral hygiene. However, with the improvement of people's quality of life and the increasing attention to oral health, the simple basic cleaning function can no longer meet the needs of consumers. More comprehensive oral health care effects, such as preventing caries and inhibiting the formation of dental plaque, have become the goals pursued by consumers. Therefore, it is particularly important to develop a new type of safe toothpaste that has the effects of preventing caries and inhibiting dental plaque. Summary of the invention

[0003] In order to overcome the deficiencies of the prior art, the purpose of the present invention is to provide a toothpaste, specifically a method for preparing a whitening toothpaste that prevents caries and inhibits dental plaque. This is achieved through the following technical solutions:

[0004] In a first aspect, the present invention provides a method for preparing toothpaste, the specific method being:

[0005] S1. Prepare Chinese medicine extract: weigh 20-25g licorice, 15-20g honeysuckle, 15-20g dandelion, 10-12g gallnut, 8-12g lily, 5-7g Sterculia lychnophora, clean, dry and crush; mix the above Chinese medicine powders evenly, add 8-10 times ethanol, put them into an ultrasonic machine for ultrasonic extraction for 20 minutes, repeat the extraction 3 times, and concentrate under reduced pressure until the ethanol content is less than 0.1% to obtain a Chinese medicine extract;

[0006] S2, preparation of peach kernel hydrolysate: 100g peach kernel was taken, crushed, added with 300mL water, stirred evenly, the temperature was adjusted to 45°C, the pH was 5.2, 2.0-2.5g complex enzyme was added, and the enzymolysis was performed for 5h. After the enzyme was inactivated at high temperature, the mixture was centrifuged in a centrifuge for 15min, and the upper layer was taken to obtain peach kernel hydrolysate;

[0007] S3, preparation of basic paste: 25-30g of zeolite and 3-5g of silicon dioxide are mixed evenly in a mixer to obtain a basic paste;

[0008] S4, preparation of active ingredient solution: 1-2g of calcium L-aspartate is added to 15-20g of water, stirred at 50°C until the calcium L-aspartate is completely dissolved, 20-30g of Chinese medicine extract and 5-10g of peach kernel enzymatic hydrolysis are mixed evenly, poured into the prepared calcium L-aspartate solution, mixed evenly, and then 0.1-0.12g of sodium benzoate and 5-8g of sorbitol are added, stirred evenly, to obtain an active ingredient solution;

[0009] S5. Preparation of toothpaste: at 60° C., slowly add the active ingredient solution into the base paste, continue stirring for 15-20 min, pour the stirred paste into a grinding dish, grind for 15 min, let stand for 12 h, centrifuge and degas to obtain the toothpaste of the present invention.

[0010] Preferably, the volume fraction of ethanol in step S1 is 65%.

[0011] Preferably, the temperature of ultrasonic extraction in step S1 is 35°C and the power is 240W.

[0012] Preferably: the Chinese medicine in step S1 and the peach kernel in step S2 are crushed into 100 mesh particles.

[0013] Preferably, the complex enzyme in step S2 comprises neutral protease and pectinase, wherein the enzyme activity of the neutral protease is 100,000 U / g, and the enzyme activity of the pectinase is 50,000 U / g.

[0014] Preferably, in step S2, the mass ratio of neutral protease to pectinase is 3:2.

[0015] Preferably, the centrifugal speed in step S2 is 4000 r / min and the time is 15 min.

[0016] Preferably, the centrifugal speed in step S5 is 4000 r / min and the time is 15 min.

[0017] In a second aspect, the toothpaste prepared by the present invention has the effects of preventing caries, inhibiting dental plaque and improving periodontitis.

[0018] The present invention has the following beneficial effects:

[0019] 1. The present invention uses liquorice extract, gallnut extract and honeysuckle extract to work synergistically and has the effect of preventing dental caries.

[0020] 2. The present invention uses the sterculia lychnophora extract and the peach kernel enzymatic hydrolysate to work synergistically, which has the effect of improving periodontitis.

[0021] 3. The present invention uses honeysuckle extract, gallnut extract and dandelion extract to work synergistically, which has the effect of inhibiting dental plaque, thereby achieving the purpose of whitening teeth.

[0022] 4. The present invention adopts calcium L-aspartate as an active ingredient, which significantly enhances the cleaning efficiency of toothpaste and effectively reduces the risk of tartar formation.

[0023] 5. The toothpaste formula prepared by the present invention strictly controls the dosage of each ingredient, ensures the safety of the product, and provides consumers with a healthy and safe oral care option. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a comparison chart of the GI and GBI indexes of mice in Example 2 group and Comparative Example 4-5 groups.

[0025] Figure 2 These are the dental plaque scoring results of beagles in Example 3 and Comparative Examples 6-8.

[0026] Figure 3 It is a physical diagram of the toothpaste of the present invention. DETAILED DESCRIPTION

[0027] The following describes the embodiments of the present invention by specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0028] Licorice, honeysuckle, dandelion, gallnut, lily, Sterculia lychnophora, and peach kernel were purchased from Anhui Kangweifu Pharmaceutical Co., Ltd.; L-calcium aspartate was purchased from Nanjing Ximenuo Biotechnology Co., Ltd.; neutral protease and pectinase were purchased from Zhengzhou Mingxin Food Ingredients Co., Ltd.

[0029] Example 1

[0030] This example proves that the liquorice extract, the gallnut extract, and the honeysuckle extract work synergistically and have the effect of preventing dental caries.

[0031] This embodiment provides a toothpaste for preventing caries and inhibiting dental plaque, and the specific steps are as follows:

[0032] S1. Preparation of Chinese medicine extract: weigh 20g licorice, 15g honeysuckle, 15g dandelion, 10g gallnut, 8g lily, and 5g Sterculia lychnophora, clean and dry, and crush to 100 mesh; mix the above Chinese medicine powders evenly, add 8 times of 65% v / v ethanol, place in an ultrasonic machine with a power of 240W at 35°C for 20min, repeat the extraction 3 times, and concentrate under reduced pressure until the ethanol content is less than 0.1%, to obtain a Chinese medicine extract;

[0033] S2, preparation of peach kernel hydrolysate: 100g peach kernel was crushed into 100 mesh particles, 300mL water was added, and after stirring, 1.2g neutral protease and 0.8g pectinase were added for enzymolysis for 5h at 45°C and pH 5.2. After the enzyme was inactivated at high temperature, the mixture was centrifuged at 4000r / min for 15min, and the upper liquid was separated to obtain peach kernel hydrolysate;

[0034] S3, preparation of basic paste: weigh 25g of zeolite and 3g of silicon dioxide and mix them evenly in a mixer to obtain a basic paste;

[0035] S4, preparation of active ingredient solution: weigh 1g of calcium L-aspartate and mix it with 15g of water, stir at 50°C until completely dissolved; take 20g of traditional Chinese medicine extract and 5g of peach kernel enzymatic hydrolysis, mix them evenly, pour them into the dissolved calcium L-aspartate solution and mix them evenly; add 0.1g of sodium benzoate and 5g of sorbitol to the above solution, stir evenly, and obtain an active ingredient solution;

[0036] S5. Preparation of toothpaste: At 60° C., slowly pour the active ingredient solution into the base paste and continue stirring for 15 minutes to ensure that all ingredients are evenly distributed; transfer the obtained paste to a grinding dish and grind it for 15 minutes to achieve further refinement and uniform distribution of the paste. After grinding, age the paste for 12 hours, centrifuge it in a centrifuge at 4000 r / min for 15 minutes, and degas to obtain the toothpaste of the present invention.

[0037] Comparative Example 1: Except that licorice was not added in step S1, other parameters were the same as those in Example 1.

[0038] Comparative Example 2: Except that no gallnut was added in step S1, other parameters were the same as those in Example 1.

[0039] Comparative Example 3: Except that honeysuckle was not added in step S1, other parameters were the same as those in Example 1.

[0040] To evaluate the inhibitory effect of toothpaste on caries-causing bacteria:

[0041] The test strains of the present invention select three kinds of cariogenic bacteria, namely Streptococcus mutans, CICC-10438, purchased from China Industrial Microbiological Culture Collection Management Center, Streptococcus sanguinis, ATCC-10556, purchased from China Industrial Microbiological Culture Collection Management Center, and Streptococcus sobrinus, ATCC-33478, purchased from Beijing Biobo Biotechnology Co., Ltd. Under a sterile environment, the bacteria are inoculated into BHI liquid culture medium at a volume ratio of 5%, and cultured under anaerobic conditions at 37°C for 24 hours. After completion, the bacteria are streaked from the liquid culture medium onto a BHI agar plate, and continue to be cultured under anaerobic conditions at 37°C for 24 hours. After the culture is completed, a typical colony is selected and transferred to the BHI liquid culture medium for culture, and then the bacterial suspension is adjusted to 1×10 7 CFU / mL for experimental use. In a sterile and anaerobic environment, the bacteria were inoculated into BHI liquid culture medium at 5% v / v and cultured anaerobically at 37°C for 24 hours. After the culture, the bacteria were transferred from the liquid culture medium to the BHI agar plate and continued to be cultured at 37°C for 24 hours. After completion, typical colonies were selected and inoculated into BHI liquid culture medium, shaken to mix and detect the absorbance at 630nm, and the concentration of the bacterial suspension was adjusted to 1×10 7 CFU / mL for subsequent experiments.

[0042] Specific experimental method: Prepare a toothpaste sample stock solution, mix the toothpaste with 98% ethanol and dilute it to a mass concentration of 40 mg / mL. In a sterile environment, preheat 20 mL of BHI agar medium to 42 ° C, add 200 μL of bacterial suspension, and ensure that it is quickly poured into a sterile culture dish with 6 Oxford cups after mixing. After the culture medium solidifies, remove the Oxford cup, add each toothpaste solution to be tested to the corresponding well, add 100 μL of the toothpaste solution to be tested to each well, and only place one toothpaste solution to be tested in each well. At the same time, take 100 μL of 98% ethanol solution as a blank group and 100 μL of diclofenac sodium solution with a concentration of 0.5 mg / mL as a positive control group. Next, the culture dish was cultured in an anaerobic environment for 24 hours at 37 ° C. After the experiment was completed, the diameter of the inhibition zone around each sample was observed and recorded. The experiment was repeated three times, and the experimental results are shown in Table 1.

[0043] Table 1

[0044] Antibacterial diameter / mm Blank Group Example 1 Group Comparative Example 1 Comparative Example 2 Comparative Example 3 Positive control group Streptococcus mutans 2.31±32 20.97±0.19 15.78±0.16 13.56±0.39 16.37±21 17.21±0.32 Streptococcus sanguinis 2.37±0.45 22.56±0.15 17.21±0.22 16.23±0.18 18.23±0.17 17.49±0.38 Streptococcus sobrinus 2.48±0.24 17.65±0.23 14.36±0.17 12.78±0.24 13.67±32 16.94±0.26

[0045] Conclusion: Dental caries is one of the most common infectious diseases in humans. It is a disease caused by the combined action of multiple microorganisms caused by specific bacteria. Streptococcus mutans, Streptococcus sanguinis and Streptococcus sobrinus are the main cariogenic bacteria that cause dental caries. They metabolize sugar to produce acid, causing tooth demineralization and thus causing dental caries. Figure 1 It is the diameter of the inhibition zone of the three cariogenic bacteria in the Example 1 group and the Comparative Examples 1-3 groups. It can be clearly seen from the figure that the diameter of the inhibition zone of the three cariogenic bacteria in the Example 1 group is significantly higher than that in the Comparative Examples 1-3 groups, and the inhibition diameter of the three cariogenic bacteria is the highest, and the prevention and treatment effect on dental caries is the best, which confirms that the licorice extract, the gallnut extract, and the honeysuckle extract have a synergistic effect and have an anti-caries effect.

[0046] Example 2

[0047] This example demonstrates that the Sterculia lychnophora extract and the peach kernel enzymatic hydrolysate act synergistically and have the efficacy of improving periodontitis.

[0048] S1. Preparation of Chinese medicine extract: weigh 23g of licorice, 18g of honeysuckle, 18g of dandelion, 11g of gallnut, 10g of lily, and 6g of Sterculia lychnophora, clean them, dry them, and crush them into 100 mesh; mix the above Chinese medicine powders evenly, add 9 times of 65% v / v ethanol, place them in an ultrasonic machine with a power of 240W for ultrasonic extraction at 35°C for 20min, repeat the extraction 3 times, and concentrate under reduced pressure until the ethanol content is less than 0.1%, to obtain a Chinese medicine extract;

[0049] S2, preparation of peach kernel hydrolysate: 100g peach kernel was crushed into 100 mesh particles, 300mL water was added, and after stirring, 1.5g neutral protease and 1.0g pectinase were added for enzymolysis at 45°C and pH 5.2 for 5h, and the enzyme was inactivated at high temperature and centrifuged at 4000r / min for 15min in a centrifuge to separate the upper liquid to obtain peach kernel hydrolysate;

[0050] S3, preparation of basic paste: weigh 28g of zeolite and 4g of silicon dioxide and mix them evenly in a mixer to obtain a basic paste;

[0051] S4, preparation of active ingredient solution: weigh 1.5g of calcium L-aspartate and mix it with 18g of water, stir at 50°C until completely dissolved; take 25g of traditional Chinese medicine extract and 8g of peach kernel enzymatic hydrolysis, mix them evenly, pour them into the dissolved calcium L-aspartate solution and mix them evenly; add 0.1g of sodium benzoate and 7g of sorbitol to the above solution, stir evenly, and obtain an active ingredient solution;

[0052] S5. Preparation of toothpaste: At 60° C., slowly pour the active ingredient solution into the base paste and continue stirring for 15 minutes to ensure that all ingredients are evenly distributed; transfer the obtained paste to a grinding dish and grind it for 15 minutes to achieve further refinement and uniform distribution of the paste. After grinding, age the paste for 12 hours, centrifuge it in a centrifuge at 4000 r / min for 15 minutes, and degas to obtain the toothpaste of the present invention.

[0053] Comparative Example 4: Except that fat dahlia was not added in step S1, other parameters were the same as those in Example 2.

[0054] Comparative Example 5: Except that peach kernel enzymatic hydrolysate was not added in step S4, other parameters were the same as those in Example 2.

[0055] Evaluation of the effect of toothpaste on improving periodontitis symptoms in mice:

[0056] Animals: BALB / c mice, all male, weighing 20±2 g, were purchased from Beijing Sibeifu Biotechnology Co., Ltd. The mice were adaptively fed for one week before the experiment.

[0057] Grouping: In the experiment, mice were randomly divided into 5 groups, with 8 mice in each group, namely, blank group, model group, Example 2 group and Comparative Example 4-5 groups.

[0058] Modeling: Except for the blank group, the rest of the mice were anesthetized by intraperitoneal injection of 1.0% sodium pentobarbital (dose of 0.15mL / 20g), the head was fixed in supine position, the maxillary area was exposed, and the orthodontic wire was passed through the gap between the second and third teeth on the right side of the mouse. Use tweezers to shape the wire so that it bends in the right direction, place the shaped wire vertically on the upper palate, and continue to adjust the shape so that the two ends of the teeth fit tightly to prevent falling off. After the excess part is cut off, observe every 2 days. Once a periodontal abscess or gingival abscess appears, it means that the periodontitis model has been successfully constructed.

[0059] Administration: The Example 2 group and the Comparative Example 4-5 groups were given 40 mg / 20 g toothpaste daily, which was evenly applied on the oral tooth surface of the mice with a cotton swab. After waiting for 30 seconds, they were allowed to drink water freely; the model group and the blank group were given an equal amount of 0.9% sodium chloride for 28 consecutive days.

[0060] After 28 days, the periodontitis-related indicators of mice in each group were evaluated, including the gingival index (GI) and gingival bleeding index (GBI). The specific scoring criteria are as follows:

[0061] Gingival Index (GI): The score ranges from 0 to 3, where 0 indicates that the gums are healthy and normal; 1 indicates that the gums are slightly swollen and the color changes, but there is no bleeding induced by the probe; 2 indicates that the gums are obviously swollen, the color is red, and there is bleeding induced by the probe; 3 indicates that the gums bleed spontaneously or ulcerate.

[0062] Gingival Bleeding Index (GBI): The score is 0 or 1, with 0 indicating no bleeding during probing and 1 indicating bleeding during probing.

[0063] Conclusion: The periodontal tissues of the mice in the blank group remained healthy without any abnormality; the mice in the model group showed obvious gingival swelling and dark red color, and easily bled when probing. Over time, the periodontal pocket depth of these mice increased, and the symptoms of periodontal inflammation further worsened; after 28 days of administration, the symptoms of periodontal inflammation in the mice in Example 2 group and Comparative Example 4-5 groups showed significant relief, among which the improvement in Example 2 group was the most significant. Figure 1 It is a comparison chart of the GI and GBI indexes of mice in Example 2 group and Comparative Example 4-5 group. Compared with the blank group, the GI and GBI indexes of mice in the model group were significantly increased. Compared with the model group, the GI and GBI indexes of mice in Example 2 group and Comparative Example 4-5 group were significantly reduced, among which the GI and GBI indexes of mice in Example 2 group were the lowest. In the study of periodontitis, GI and GBI indexes are two important indicators for evaluating the status of periodontal inflammation. These indicators are closely related to the symptoms of periodontitis and can reflect the health status of periodontal tissues. It can be confirmed that the sterculia lychnophora extract and the peach kernel enzymatic hydrolyzate act synergistically and have the effect of improving periodontitis.

[0064] Example 3

[0065] This example demonstrates that the honeysuckle extract, the Chinese nut extract and the dandelion extract work synergistically and have the effect of inhibiting dental plaque.

[0066] S1. Preparation of Chinese medicine extract: weigh 25g of licorice, 20g of honeysuckle, 20g of dandelion, 12g of gallnut, 12g of lily, and 7g of Sterculia lychnophora, clean them, dry them, and crush them into 100 mesh; mix the above Chinese medicine powders evenly, add 10 times of 65% v / v ethanol, place them in an ultrasonic machine with a power of 240W at 35°C for ultrasonic extraction for 20min, repeat the extraction 3 times, and concentrate under reduced pressure until the ethanol content is less than 0.1%, to obtain a Chinese medicine extract;

[0067] S2, preparation of peach kernel hydrolysate: 100g peach kernel was crushed into 100 mesh particles, 300mL water was added, and after stirring, 1.5g neutral protease and 1.0g pectinase were added for enzymolysis at 45°C and pH 5.2 for 5h, and the enzyme was inactivated at high temperature and centrifuged at 4000r / min for 15min in a centrifuge to separate the upper liquid to obtain peach kernel hydrolysate;

[0068] S3, preparation of basic paste: weigh 30g of zeolite and 5g of silicon dioxide and mix them evenly in a mixer to obtain a basic paste;

[0069] S4, preparation of active ingredient solution: weigh 2g of calcium L-aspartate and mix it with 20g of water, and stir at 50°C until completely dissolved; take 30g of traditional Chinese medicine extract and 10g of peach kernel enzymatic hydrolysis, mix them evenly, pour them into the dissolved calcium L-aspartate solution and mix them evenly; add 0.12g of sodium benzoate and 8g of sorbitol to the above solution, stir evenly, and obtain an active ingredient solution;

[0070] S5. Preparation of toothpaste: At 60° C., slowly pour the active ingredient solution into the base paste and continue stirring for 15 minutes to ensure that all ingredients are evenly distributed; transfer the obtained paste to a grinding dish and grind it for 15 minutes to achieve further refinement and uniform distribution of the paste. After grinding, age the paste for 12 hours, centrifuge it in a centrifuge at 4000 r / min for 15 minutes, and degas to obtain the toothpaste of the present invention.

[0071] Comparative Example 6: Except that honeysuckle was not added in step S1, other parameters were the same as those in Example 3.

[0072] Comparative Example 7: Except that the gallnut was not added in step S1, the other parameters were the same as those in Example 3.

[0073] Comparative Example 8: Except that dandelion is not added in step S1, other parameters are the same as those of Example 3.

[0074] Evaluation of the effect of toothpaste on the inhibition of dental plaque in beagle dogs:

[0075] Animals: 25 beagles weighing 12±2kg were selected and provided by Jinan Jinfeng Experimental Animal Co., Ltd. Before the experiment, each dog underwent an oral health examination to ensure that there were no periodontal problems and that the bite was correct, and a comprehensive physical examination and blood biochemical tests were completed. At the same time, all dogs had not received antibiotics or anti-inflammatory drugs 7 days before the experiment.

[0076] Grouping: 25 beagle dogs were randomly divided into a blank group, Example 3 group, and Comparative Example 6-8 groups, with 5 dogs in each group.

[0077] Drug administration: After the experiment started, all beagle dogs were cleaned and polished. Beagle dogs in the blank group were fed with normal dog food; beagle dogs were given 5g / 12kg toothpaste 30 minutes after each meal, which was evenly applied on the surface of the dog's oral teeth with a cotton swab. After waiting for half a minute, they were allowed to drink water freely. This was done once in the morning and evening every day for 28 days.

[0078] The experimental dogs were subjected to tooth cleaning and polishing treatment, and the blank group was fed as usual. 30 minutes after the meal, 5g / 12kg toothpaste was taken for the Example 3 group and the Comparative Example 6-8 groups, and evenly applied on the teeth with a cotton swab, and allowed to drink water freely after standing for half a minute. Twice a day, for 28 days.

[0079] On the 29th day of the experiment, the dental plaque condition of each beagle's teeth was evaluated in detail. The teeth were stained with a plaque disclosing agent and then rinsed with pure water for easy observation. The dental plaque score was based on the following two dimensions:

[0080] Coverage area:

[0081] 0 points: no dental plaque on the tooth surface; 1 point: dotted plaque at the gingival margin of the tooth neck; 2 points: the width of the plaque at the tooth neck does not exceed 1 mm; 3 points: the plaque width exceeds 1 mm, but does not exceed 1 / 3 of the tooth surface; 4 points: the plaque coverage area occupies 1 / 3 to 2 / 3 of the tooth surface; 5 points: the plaque coverage area exceeds 2 / 3 of the tooth surface.

[0082] b.Thickness:

[0083] 0 points - no color change; 1 point - mild, dotted or light red; 2 points - moderate, red; 3 points - severe, dark red.

[0084] The formula for calculating dental plaque score is: score = coverage area × thickness; the experimental results are as follows Figure 2 shown.

[0085] in conclusion: Figure 2 This is the dental plaque score result of beagles on the 29th day of the experiment. At the beginning of the experiment, all beagles were subjected to teeth cleaning and polishing, so the dental plaque scores of beagles were all 0 at the beginning of the experiment; on the 29th day of the experiment, the dental plaque scores of the blank group, Example 3 group 5.8 points, Comparative Example 7 group 7.2 points, Comparative Example 8 group 7.6 points, Comparative Example 9 group 7.8 points were respectively, compared with the blank group, the dental plaque scores of beagles in Example 3 group and Comparative Example 6-8 groups were significantly reduced, compared with Comparative Example 6-8 groups, the dental plaque score of beagles in Example 3 group was the lowest, confirming that the honeysuckle extract, the gallnut extract and the dandelion extract act synergistically and have the effect of inhibiting dental plaque.

[0086] The above-mentioned embodiments only express the specific implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the protection scope of the present invention. It should be pointed out that, for those skilled in the art, without departing from the concept of the present invention, any other changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principle of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A whitening toothpaste for preventing caries and inhibiting dental plaque, characterized in that: The toothpaste comprises, by weight, 25-30 parts of zeolite, 3-5 parts of silicon dioxide, 1-2 parts of calcium L-aspartate, 20-30 parts of traditional Chinese medicine extract, 0.1-0.12 parts of sodium benzoate, 5-8 parts of sorbitol, 5-10 parts of peach kernel enzymatic hydrolyzate and 15-20 parts of water.

2. A whitening toothpaste for preventing caries and inhibiting dental plaque according to claim 1, characterized in that: The toothpaste Chinese medicine extract comprises 20-25 parts of liquorice, 15-20 parts of honeysuckle, 15-20 parts of dandelion, 10-12 parts of gallnut, 8-12 parts of lily and 5-7 parts of Sterculia lychnophora by weight.

3. The anti-caries and anti-plaque whitening toothpaste according to claim 1, characterized in that: The specific preparation method of the peach kernel enzymatic hydrolysate in the toothpaste is as follows: 100g of peach kernels are crushed, 300mL of water is added, and the mixture is stirred evenly. At 45°C, the pH is adjusted to 5.2, 2.0-2.5g of complex enzyme is added, enzymolysis is performed for 5h, centrifuged for 15min, and the upper clear liquid is taken to obtain the peach kernel enzymatic hydrolysate.

4. The anti-caries and anti-plaque whitening toothpaste according to claim 1, characterized in that: The toothpaste has the functions of preventing caries, inhibiting dental plaque and improving periodontitis.

5. A method for preparing a caries-preventing and plaque-inhibiting whitening toothpaste according to any one of claims 1 to 4, characterized in that: The specific preparation method of the toothpaste is as follows: S1. Preparation of Chinese medicine extract: weigh 20-25g of licorice, 15-20g of honeysuckle, 15-20g of dandelion, 10-12g of gallnut, 8-12g of lily, and 5-7g of Sterculia lychnophora, clean them, dry them, and crush them; mix the above Chinese medicine powders evenly, add 8-10 times of ethanol, and perform ultrasonic extraction for 20min, repeat the extraction 3 times, combine the filtrate, and concentrate it under reduced pressure until the ethanol content is less than 0.1%, so as to obtain the Chinese medicine extract; S2, preparation of peach kernel hydrolysate: 100g peach kernel was crushed, 300mL water was added, stirred evenly, pH was adjusted to 5.2 at 45°C, 2.0-2.5g complex enzyme was added, enzymolysis was performed for 5h, centrifuged for 15min, and the supernatant was taken to obtain peach kernel hydrolysate; S3, preparation of basic paste: take 25-30g of zeolite and 3-5g of silicon dioxide and mix them evenly in a mixer to obtain a basic paste; S4, preparation of active ingredient solution: 1-2g L-aspartate calcium is mixed with 15-20g water, stirred at 50°C until dissolved, 20-30g of Chinese medicine extract and 5-10g of peach kernel enzymatic hydrolyzate are mixed evenly, added to the L-aspartate calcium solution, stirred evenly, and then 0.1-0.12g of sodium benzoate and 5-8g of sorbitol are added, stirred evenly, to obtain an active ingredient solution; S5. Preparation of toothpaste: At 60° C., slowly add the active ingredient solution into the base paste, continue stirring for 15-20 min, pour the evenly stirred paste into a grinding dish, grind for 15 min, age for 12 h, centrifuge and degas to obtain the toothpaste.

6. The method for preparing a caries-preventing and plaque-inhibiting whitening toothpaste according to claim 5, characterized in that: The volume fraction of ethanol in step S1 is 65%.

7. The method for preparing a caries-preventing and plaque-inhibiting whitening toothpaste according to claim 6, characterized in that: The temperature of the ultrasonic extraction in step S1 is 35° C. and the power is 240W.

8. The method for preparing a caries-preventing and plaque-inhibiting whitening toothpaste according to claim 7, characterized in that: The composite enzyme in step S2 includes neutral protease and pectinase, wherein the enzyme activity of the neutral protease is 100,000 U / g, and the enzyme activity of the pectinase is 50,000 U / g.

9. The method for preparing a caries-preventing and plaque-inhibiting whitening toothpaste according to claim 8, characterized in that: The mass ratio of neutral protease to pectinase in step S2 is 3:

2.

10. The method for preparing a caries-preventing and plaque-inhibiting whitening toothpaste according to claim 5, characterized in that: The centrifugal speed in step S2 and step S5 is 4000 r / min and the time is 15 min.

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