Formula of anti-gout composition for assisting in reducing uric acid
By assisting the excretion of uric acid by compositions such as celery seed extract, the problem of gout caused by hyperuricemia is solved, and the effect of effectively reducing uric acid levels and preventing gout is achieved.
Patent Information
- Application Number
- CN202510102099.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-22
- Publication Date
- 2025-05-13
AI Technical Summary
Hyperuricemia leads to the onset of gout, and the prior art is difficult to effectively assist in the excretion of uric acid and reduce uric acid levels.
Through the combination of celery seed extract, luteolin, vine tea, goose carnosine, dandelion extract, and needle cherry extract, the secondary absorption and excretion of uric acid by the renal tubular epithelium is assisted to reduce the uric acid level.
It effectively assists the body in excretion of uric acid, reduces uric acid levels, and prevents the occurrence of gout.
Smart Images
Figure FT_1 
Figure FT_2 
Figure FT_3
Abstract
Description
Technical Field
[0001] The invention relates to a formula of a composition for assisting in lowering uric acid and preventing gout, and in particular to the field of health food for lowering uric acid and preventing gout. Background Art
[0002] With the development of society, people's quality of life is constantly improving, and the food is becoming more and more abundant. It is inevitable to eat high-salt, high-sugar, high-fat, high-protein and other foods. At the same time, young people nowadays exercise less, which can easily cause the body's secretion disorder. Over time, it is easy to cause symptoms such as high uric acid. High uric acid is a metabolic disease caused by the increase of uric acid in the blood due to metabolic disorders of substances called purines in the human body. The basis of gout is hyperuricemia. When the concentration of uric acid in the blood is higher than normal, it will precipitate in the form of urate crystals and deposit in the joints, cardiovascular, kidneys and pancreas. It usually initially manifests as sudden joint redness, swelling, heat and pain. Uric acid itself is a human purine metabolite generated by hypoxanthine and xanthine under the action of xanthine oxidase. When the body lacks urate oxidase, it cannot be decomposed into soluble allantoin, which leads to the formation of hyperuricemia. The celery seed extract composition is an alkaline substance, which can reduce the secondary absorption of uric acid by renal tubular epithelial cells, assist in accelerating the excretion of uric acid, and play a certain role in reducing uric acid.
[0003] Therefore, the composition of the present invention has been developed, which can effectively assist the secondary absorption of uric acid by the renal tubular epithelium and can well assist the excretion of uric acid. The composition of the present invention can be made into corresponding tablets, capsules, granules, pills or oral liquids according to the applicable population and ease of carrying. Summary of the invention
[0004] The purpose of the present invention is to provide a formula of a composition for assisting in lowering uric acid and preventing gout. The composition for assisting in lowering uric acid and preventing gout is mainly prepared by fully mixing and stirring celery seed extract, luteolin, rattan tea, anserine, dandelion extract, and acerola cherry extract in a stirring pot. The multiple ingredients complement each other and effectively assist the human body in excreting uric acid, thereby preventing the occurrence of gout symptoms.
[0005] Through long-term experiments, it was found that the uric acid lowering and anti-gout composition is mainly composed of celery seed extract (0.58-0.65), luteolin (0.015-0.024), rattan tea (0.076-0.085), anserine (0.094-0.11), dandelion extract (0.092-0.115), and acerola cherry extract (0.09-0.118). These compositions are in these ranges and proportions, which can assist the human body in clearing uric acid produced by daily metabolism. At the same time, the celery seed extract in the uric acid lowering composition helps the human renal tubular epithelial cells to absorb uric acid for the second time, which can minimize the induction of gout by uric acid in the human body.
[0006] Unless otherwise specified, the composition described in the present invention and the raw materials used can all be obtained through conventional market purchasing channels: celery seed extract was purchased from Xi'an Shangcheng Biotechnology Co., Ltd., corresponding to the brand name Chongde Shangcheng; luteolin was purchased from Xi'an Yuze Biotechnology Co., Ltd., corresponding to the brand Yuze Biology; rattan tea was purchased from Hubei Youfenglai Selenium Ecological Agriculture Technology Co., Ltd., corresponding to the brand Shanhuo Catcher; anserine was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., corresponding to the brand Aladdin; dandelion extract was purchased from Sanyuan Tianyu Biological Products Co., Ltd., corresponding to the brand Tianyu TY; acerola cherry extract was purchased from Shaanxi Snot Biotechnology Co., Ltd., corresponding to the brand Silk Road Muxia. BRIEF DESCRIPTION OF THE DRAWINGS
[0007] Figure 1: Corresponding implementation data table of functional formula ingredients of different liver-protecting and alcohol-relieving compositions, P<0.05.
[0008] Figure 2: In the experiments of different embodiments, the samples with the maximum safety value of 1000 μg / mL were used as the positive control group. The experimental group was more effective than the normal group, P<0.05.
[0009] Figure 3: The mean ± standard deviation of zebrafish uric acid values in different experiments, and the uric acid values of the sample control group lower than the positive control group.
[0010] To achieve the above-mentioned purpose, the present invention provides a formula of an auxiliary uric acid lowering composition, comprising the following ingredients: celery seed extract, luteolin, rattan tea, anserine, dandelion extract, and acerola cherry extract, and the corresponding implementation proportions are (0.42-0.72), (0.01-0.029), (0.068-0.095), (0.084-0.15), (0.082-0.135), and (0.08-0.142).
[0011] The celery seed extract and other compositions are described in the following examples:
[0012] Furthermore, the proportions of the embodiment are: celery seed extract (0.42), luteolin (0.01), rattan tea (0.068), anserine (0.084), dandelion extract (0.082), acerola cherry extract (0.08), wherein the luteolin composition has a molecular weight content of <6000 Daltons, and the test results show that the P value is <0.05.
[0013] Furthermore, the example is divided into groups: celery seed extract (0.45), luteolin (0.012), rattan tea (0.06), anserine (0.086), dandelion extract (0.088), and acerola cherry extract (0.082). The luteolin composition has a molecular weight content of <6000 Daltons, and the test result shows that the P value is <0.05.
[0014] Furthermore, the example is divided into groups: celery seed extract (0.5), luteolin (0.015), rattan tea (0.072), anserine (0.088), dandelion extract (0.092), and acerola cherry extract (0.09), wherein the luteolin composition has a molecular weight content of <6000 Daltons, and the test result shows that the P value is <0.04.
[0015] Furthermore, the example is divided into groups: celery seed extract (0.58), luteolin (0.015), rattan tea (0.076), anserine (0.094), dandelion extract (0.092), and acerola cherry extract (0.09), wherein the luteolin composition has a molecular weight content of <6000 Daltons, and the test result shows that the P value is <0.02.
[0016] Furthermore, the example is divided into groups: celery seed extract (0.62), luteolin (0.022), rattan tea (0.082), anserine (0.01), dandelion extract (0.11), and acerola cherry extract (0.11), wherein the luteolin composition has a molecular weight content of <6000 Daltons, and the test result shows that the P value is <0.01.
[0017] Furthermore, the example is divided into groups: celery seed extract (0.65), luteolin (0.024), rattan tea (0.085), anserine (0.11), dandelion extract (0.115), and acerola cherry extract (0.118), wherein the luteolin composition has a molecular weight content of <6000 Daltons, and the test result shows that the P value is <0.02.
[0018] Furthermore, the embodiment is divided into groups according to the proportions: celery seed extract (0.72), luteolin (0.029), rattan tea (0.095), anserine (0.15), dandelion extract (0.135), and acerola cherry extract (0.142). The luteolin composition has a molecular weight content of <6000 Daltons, and the detection result P value is <0.04.
[0019] The functional formula of the liver-protecting and alcohol-detoxifying composition can achieve the best liver-protecting and alcohol-detoxifying effect by having the following proportions of celery seed extract, luteolin, rattan tea, anserine, dandelion extract and acerola cherry extract: (0.58-0.65): (0.015-0.024): (0.076-0.085): (0.094-0.11): (0.092-0.115): (0.09-0.118). Specific implementation plan
[0020] The specific implementation scheme selected zebrafish as the experimental subject. In the biochemical chain of uric acid formation, xanthine oxidoreductase catalyzes the last two steps of the reaction, metabolizing hypoxanthine into xanthine, and further decomposing xanthine into the final product uric acid. Uric acid oxidase exists in zebrafish and most mammals. It is an enzyme that efficiently reduces uric acid levels. It can decompose uric acid into highly water-soluble allantoin and excrete it with urine. However, humans do not have uric acid oxidase, so the establishment of a zebrafish hyperuricemia model requires the addition of potassium oxonate to inhibit the activity of uric acid oxidase, while increasing xanthine, a precursor substance for uric acid formation, to increase the synthesis of uric acid. After testing with a uric acid detector, the uric acid value of zebrafish with hyperuricemia will be significantly higher than that of normal zebrafish, and the positive drug is allopurinol.
[0021] The experimental detection instruments and reagents include: centrifuge, ultrasonic cell disruptor, xanthine (CAS: 69-89-6), potassium oxonate (CAS: 2207-75-2), allopurinol, and Kefu uric acid detector (UA03-C, Kefu Medical Technology Co., Ltd.).
[0022] The zebrafish are adult fish that the company has specially selected according to experimental standards for breeding and reproduction methods, and are raised in an environment that simulates the wild. In order to make the experiment more significant, the experimental zebrafish are specially selected from adult fish that are five days old after fertilization for the experiment.
[0023] The experimental zebrafish were divided into seven groups during the experiment, with 32 zebrafish in each group. The experimental principle was mainly based on the urate oxidase in zebrafish, which is an enzyme that efficiently reduces uric acid levels and can decompose uric acid into highly water-soluble allantoin and excrete it with urine. Therefore, to establish a zebrafish hyperuricemia model, potassium oxonate needs to be added to inhibit the activity of urate oxidase, and at the same time, xanthine, a precursor substance for the formation of uric acid, is increased to increase the synthesis of uric acid. After testing with a uric acid detector, the uric acid value of zebrafish with hyperuricemia will be significantly higher than that of normal zebrafish, and the positive drug is allopurinol.
[0024] The experimental zebrafish were divided into seven groups, each of which was further divided into four experimental groups. The normal control group was not treated, the model control group was added with xanthine and potassium oxonate, and the positive control group and the sample treatment group were added with allopurinol and the sample solution to be tested at the same time as xanthine and potassium oxonate. After culturing for a period of time, the zebrafish were crushed and the tissue fluid was taken to detect the uric acid value.
[0025] In Example 1, through the above experimental method, a composition containing celery seed extract (0.42), luteolin (0.01), rattan tea (0.068), anserine (0.084), dandelion extract (0.082), and acerola cherry extract (0.08) was dissolved in water and a sample with a maximum safe concentration of 1000 μg / mL was taken. The corresponding P value <0.05 was obtained through experimental data analysis. Compared with the uric acid value of the model control group, the comparison results showed effectiveness.
[0026] In Example 2, through the above experimental method, the corresponding composition of celery seed extract (0.45), luteolin (0.012), rattan tea (0.06), anserine (0.086), dandelion extract (0.088), and acerola cherry extract (0.082) was dissolved in water and a sample with the maximum safe value of 1000 μg / mL concentration was taken. The corresponding P value <0.05 was obtained through experimental data analysis. Compared with the uric acid value of the model control group, the comparison results showed effectiveness.
[0027] In Example 3, through the above experimental method, the corresponding composition of celery seed extract (0.5), luteolin (0.015), rattan tea (0.072), anserine (0.088), dandelion extract (0.092), and acerola cherry extract (0.09) was dissolved in water and a sample with a maximum safe concentration of 1000 μg / mL was taken. The corresponding P value <0.04 was obtained through experimental data analysis. Compared with the uric acid value of the model control group, the comparison results showed effectiveness.
[0028] In Example 4, through the above experimental method, the corresponding celery seed extract (0.58), luteolin (0.015), rattan tea (0.076), anserine (0.094), dandelion extract (0.092), and acerola cherry extract (0.09) were dissolved in water and samples with the maximum safe value of 1000 μg / mL concentration were taken. The corresponding P value <0.02 was obtained through experimental data analysis. Compared with the uric acid value of the model control group, the comparison results showed a significant effect.
[0029] In Example 5, by using the above experimental method, a composition of celery seed extract (0.62), luteolin (0.022), rattan tea (0.082), anserine (0.01), dandelion extract (0.11), and acerola cherry extract (0.11) was dissolved in water and a sample with a maximum safe concentration of 1000 μg / mL was taken. The corresponding P value <0.01 was obtained through experimental data analysis. Compared with the uric acid value of the model control group, the comparison results showed a significant effect.
[0030] In Example 6, through the above experimental method, the corresponding composition of celery seed extract (0.65), luteolin (0.024), rattan tea (0.085), anserine (0.11), dandelion extract (0.115), and acerola cherry extract (0.118) was dissolved in water and a sample with a maximum safe concentration of 1000 μg / mL was taken. The corresponding P value <0.02 was obtained through experimental data analysis. Compared with the uric acid value of the model control group, the comparison results showed a significant effect.
[0031] In Example 7, through the above experimental method, the corresponding composition of celery seed extract (0.72), luteolin (0.029), rattan tea (0.095), anserine (0.15), dandelion extract (0.135), and acerola cherry extract (0.142) was dissolved in water and a sample with a maximum safe value of 1000 μg / mL concentration was taken. The corresponding P value <0.04 was obtained through experimental data analysis. Compared with the uric acid value of the model control group, the comparison results showed effectiveness.
[0032] The data analysis was mainly performed using SPSS26 software, and GraphPad Prism8 was used for drawing. The data statistics were expressed as mean ± standard deviation. The experimental results were tested for normal distribution using SPSS software. If the data conformed to the normal distribution, the T parameter test or analysis of variance (ANOVA) was used for significance. If it did not conform to the normal distribution, a non-parametric test was used.
[0033] The corresponding numerical comparison results produced by the experiment show that the yolk nest area of the sample treatment group is significantly reduced or absent when compared with the positive control group, indicating that the test samples in the sample treatment group have obvious liver protection function.
[0034] The test results show that the uric acid-lowering and gout-preventing functional composition disclosed in the present invention has good results in experimental numbers 1-7 and test results 4-6. It can be seen from the experimental cases implemented above that the uric acid-lowering and gout-preventing functional composition disclosed in the present invention corresponding to the celery seed extract (0.58-0.65), luteolin (0.015-0.024), rattan tea (0.076-0.085), anserine (0.094-0.11), dandelion extract (0.092-0.115), and acerola cherry extract (0.09-0.118) composition range ratio embodiment has the best effect.
[0035] Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified in proportion or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.
Claims
1. A formula for a composition for assisting in lowering uric acid and preventing gout, wherein the main ingredients of the composition for lowering uric acid and preventing gout are celery seed extract, luteolin, rattan tea, anserine, dandelion extract, and acerola cherry extract, which work together to form a composition in relative proportions through physical stirring and mixing.
2. In the formula of the auxiliary uric acid lowering and gout prevention composition: the corresponding proportions of celery seed extract, luteolin, rattan tea, anserine, dandelion extract, and acerola cherry extract are (0.58-0.65): (0.015-0.024): (0.076-0.085): (0.094-0.11): (0.092-0.115): (0.09-0.118); the combination ratio is evenly mixed by sufficient physical stirring to obtain a composition with an optimal ratio.
3. According to the formula of the auxiliary uric acid lowering and gout prevention composition of claim, wherein Luteolin, the molecular weight of this component is <6000Da.
4. The composition for assisting in lowering uric acid and preventing gout according to claim 1, characterized in that Any one or more of 1-6 in synergistic combination can help lower uric acid and have a good preventive effect on gout.
5. The formula of the auxiliary uric acid lowering and gout prevention composition according to claim, characterized in that: The uric acid lowering composition accounts for 85%-95% of the total ingredients.
6. The auxiliary uric acid lowering and gout prevention composition according to claim 1, characterized in that: The formula composition is easily soluble in water and easily absorbed and transformed by the human body.
7. According to the formula of the auxiliary uric acid lowering and gout preventing composition of claim, a certain proportion of the composition is evenly mixed and stirred to form a granular composition containing a corresponding amount, and the composition can also be made into corresponding tablets, capsules, granules, pills or oral liquids.