Preparation method of green liquid fertilizer and application of green liquid fertilizer in resistance to low-temperature and low-illumination stress

Abstract

The invention relates to the technical field of fertilizer production, in particular to a preparation method of a green liquid fertilizer and application of the green liquid fertilizer in resistance to low-temperature and low-illumination stress. The preparation method of the green liquid fertilizer comprises the following steps: (1) mixing a pig manure organic fertilizer with a solvent, and adding sand to obtain a fertilizer liquid; (2) mixing the fertilizer liquid with a compound bio-enzyme preparation, and performing enzymolysis to obtain mixed fertilizer liquid; and (3) carrying out nutrition enhancement on the mixed fertilizer liquid, and standing to obtain supernate, namely the green liquid fertilizer. The green liquid fertilizer prepared by the preparation method provided by the invention is comprehensive and balanced in nutrition, can effectively improve the capability of crops to deal with low-temperature and low-illumination weather, and is suitable for various application modes such as seed soaking, root irrigation, foliage spraying and the like.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizer production, and in particular to a preparation method of a green liquid fertilizer and an application thereof in resistance to low temperature and low light stress. Background Art

[0004] In recent years, some provinces and regions have experienced continuous rainy weather, low temperature and low light weather, which has had a huge impact on the normal growth and development of crops. The production of grain crops such as wheat and corn has dropped significantly, and the root rot, stem base rot, growth point necrosis, flower drop, fruit drop, and fruit cracking of vegetables, fruits and other plants are very common; low temperature and low light have caused crops to grow weakly, disease resistance has dropped sharply, and pests and diseases have occurred frequently. Crop growth and development mainly rely on three major systems: photosynthesis, respiration, and endogenous hormone growth regulation. Low temperature and low light weaken photosynthesis and respiration, and hormone levels have dropped, causing poor crop growth, weakened resistance, and reduced yield and quality. At present, agricultural measures to deal with low temperature and low light weather have not yet been established. In addition, the neglect of organic fertilizer input and the improper use of highly active chemical fertilizers, biostimulants, and plant growth regulators have aggravated agricultural production risks and economic losses.

[0005] The main raw materials of the current mainstream organic fertilizers in the market are cow dung, sheep dung, chicken dung, etc. Due to the characteristics of the raw materials themselves, such as cool or hot, high cellulose, nutrient deficiency, high saline-alkali content, etc., improper use will cause great losses to agricultural production. In addition, the ingredients of the current mainstream organic water-soluble fertilizers in the market are humic acid, amino acids, alginic acid and other biostimulants, which can stimulate crop growth in a short period of time. In low-temperature and low-light weather, crops themselves have few photosynthetic products. Since crop growth is stimulated and a large amount of sugar in the crop body is consumed, the use of mainstream organic water-soluble fertilizers in the market will reduce crop resistance. At present, there is no product that uses commercial organic fertilizers to prepare water-soluble fertilizers resistant to low-temperature and low-light weather.

[0006] In order to effectively deal with low temperature and low light weather and minimize disaster losses, it is necessary to fully understand the growth and development laws of crops under low temperature and low light weather, which involves knowledge of disciplines such as plant physiology, plant nutrition, and plant biochemistry, and at the same time take effective measures to deal with it. Summary of the invention

[0007] Purpose of the invention: The technical problem to be solved by the present invention is to provide a preparation method of a green liquid fertilizer and its application in resistance to low temperature and low light stress in view of the deficiencies in the prior art.

[0008] In order to solve the above-mentioned technical problems, the present invention discloses a preparation method of green liquid fertilizer and its application in anti-low temperature and oligo-light stress, so as to solve the problem of normal growth of crops under low temperature and oligo-light weather. The preparation method described in the present invention uses high-quality commercial organic fertilizer as raw material, and prepares rich soluble sugars, multiple free amino acids and mineral nutrients through pure water (or deionized water or rainwater) soaking, stirring, composite biological enzymolysis, nutrient element strengthening and other processes, which can significantly enhance root respiration and mineral nutrient absorption, promote functional leaf photosynthesis, coordinate crop C / N ratio, enhance crop carbon and nitrogen metabolism ability, and is a new type of liquid organic fertilizer that can effectively improve the ability of crops to cope with low temperature and oligo-light weather. The liquid organic fertilizer has comprehensive and balanced nutrition, will not cause phenomena such as crop leggy growth, vigorous growth, premature aging, etc., is suitable for soaking seeds, root irrigation, foliar spraying, etc., and at the same time, the land is sprayed after rotary tillage before sowing, with the characteristics of increasing soil organic nutrition, effectively activating soil microorganisms, and improving soil looseness and air permeability. This method has a simple process flow, strong operability, good effect, clean and pollution-free, and is easy to promote and apply. It is suitable for various crops such as grain crops, vegetables, melons, and fruit trees that are affected by low temperature and low light climates, and can solve the shortcomings of soil compaction, poor crop resistance, and poor yield quality traits. The specific technical solution is as follows:

[0009] A method for preparing green liquid fertilizer comprises the following steps:

[0010] (1) mixing pig manure organic fertilizer and solvent, adding sand, and obtaining fertilizer liquid;

[0011] (2) mixing the fertilizer liquid with a composite biological enzyme preparation and obtaining a mixed fertilizer liquid by enzymolysis;

[0012] (3) Add FeSO4, MnSO4, ZnSO4, H3BO3, citric acid, glutamic acid, arginine, aspartic acid and choline chloride to the mixed fertilizer liquid for nutrient fortification, mix well and let stand, the supernatant is the green liquid fertilizer. The citric acid is used to chelate trace elements and play a role in corrosion prevention.

[0013] Wherein, in step (1), the pig manure organic fertilizer is commercially available pig manure organic fertilizer, with a pH of 5.5-7.5 and an electrical conductivity EC ≤ 2.0 dS / m; the pig manure organic fertilizer must comply with the national organic fertilizer standard (NY / T525-2021) to prevent counterfeit and inferior products. Preferably, the pig manure organic fertilizer is purchased from Hebei Devodo Fertilizer Co., Ltd.

[0014] Wherein, in step (1), the solvent is a solvent with a conductivity of <0.1dS / m, including any one of deionized water, pure water or rainwater. Deionized water or pure water is preferred. The solvent of the present invention contains no or few ions, which reduces the ion antagonism during the soaking process of the pig manure organic fertilizer and avoids the reduction of fertilizer efficiency. The deionized water, pure water or rainwater fully infiltrates the pig manure organic fertilizer, thereby improving the efficiency of the next enzymatic hydrolysis.

[0015] Wherein, in step (1), the mixing volume ratio of the pig manure organic fertilizer and the solvent is 1 to 5:1, preferably 3:1; the sand is sand powder that has passed through a 200-mesh sieve, and the added concentration of the sand in the fertilizer liquid is 0.05wt% to 0.5wt%, preferably 0.1wt%. Further preferably, the sand is used to activate silicate bacteria, and silicon and silicate bacteria can improve crop photosynthesis under low temperature and low light weather.

[0016] Preferably, step (1) is to soak the pig manure organic fertilizer with a solvent, then add sand, and continue to stir and soak for 30 minutes to obtain a fertilizer liquid.

[0017] Wherein, in step (2), the composite bio-enzyme preparation is a commercially available composite bio-enzyme preparation, which includes Bacillus subtilis viable bacteria count ≥ 2.5 billion CFU / g, Bacillus licheniformis viable bacteria count ≥ 2.5 billion CFU / g, protease ≥ 2500U / g, cellulase ≥ 1000U / g and phytase ≥ 500U / g. Preferably, the carrier of the composite bio-enzyme preparation is yeast culture and stone powder. Preferably, the composite bio-enzyme preparation is purchased from Cangzhou Xindadi Biotechnology Co., Ltd.

[0018] Wherein, in step (2), the added mass of the composite bio-enzyme preparation is 0.5% to 10% of the mass of the pig manure organic fertilizer in step (1). Preferably, it is 1.0%. The enzymolysis reaction time is 24 hours, and the enzymolysis is stirred once every 4 to 6 hours, and each stirring is 10 to 20 minutes. Preferably, the enzymolysis reaction time is 24 hours, and the stirring is once every 6 hours, and each stirring is 15 minutes.

[0019] Wherein, in step (3), the added concentration of FeSO4 is 40-60 mg / L, the added concentration of MnSO4 is 20-30 mg / L, the added concentration of ZnSO4 is 5-15 mg / L, the added concentration of H3BO3 is 1-3 mg / L, the added concentration of citric acid is 2-4 g / L, the added concentration of glutamic acid is 80-110 mg / L, the added concentration of arginine is 80-110 mg / L, the added concentration of aspartic acid is 80-110 mg / L, and the added concentration of choline chloride is 0.5-2 g / L. The preferred added concentration is the added concentration in the mixed liquid fertilizer, and more preferably 50 mg / L FeSO4, 25 mg / L MnSO4, 10 mg / L ZnSO4, 2 mg / L H3BO3, 3 g / L citric acid, 100 mg / L glutamic acid, 100 mg / L arginine, 100 mg / L aspartic acid, 1 g / L choline chloride. Further preferably, the mixing in step (3) is stirred for 30 min.

[0020] In a second aspect, the present invention provides a green liquid fertilizer prepared by the preparation method described in the first aspect. Preferably, the green liquid fertilizer comprises: soluble sugars, a variety of free amino acids, mineral nutrients, and the like.

[0021] In a third aspect, the present invention provides the use of the green liquid fertilizer described in the second aspect in enhancing the ability of crops to resist low temperature and low light stress, wherein the low temperature and low light have a temperature of 5 to 15°C and an illumination of 3000 to 3450 LX. Preferably, the crops are fruits and vegetables, more preferably tomatoes. The green liquid fertilizer has the functions of strengthening root development, promoting growth, improving photosynthetic efficiency of functional leaves, and significantly improving the ability of crops to cope with low temperature and low light weather.

[0022] In a fourth aspect, the present invention provides a method for applying the green liquid fertilizer described in the second aspect, wherein the green liquid fertilizer is applied by soil sprinkling, root irrigation or foliar spraying, wherein the dilution factor of the green liquid fertilizer during application is 200 to 500 times, preferably 200 times.

[0023] Beneficial effects: Compared with the prior art, the present invention has the following advantages:

[0024] 1. The present invention is green, environmentally friendly, and has no secondary pollution: fermented and decomposed commercial organic fertilizer is used as raw material, and after composite biological enzymolysis and addition of green nutrient elements, the green liquid fertilizer preparation process is clean and has no secondary pollution; the hydrolysis product of pig manure organic fertilizer is a group of mixed organic components, which is mild in nature and will not stimulate crop growth. After being absorbed by crops, it can significantly improve the ability of crops to withstand low temperature and low light weather.

[0025] 2. The invention has low cost: the raw materials are cheap, and growers can prepare liquid fertilizer by themselves according to this method, which can greatly save the cost of agricultural materials. Compared with the purchased liquid fertilizer, the cost can be saved by more than 90%. The current finished liquid fertilizer is expensive, which can reach 10,000 to 2 million. The preparation process of this method is simple, and the cost is only 300 yuan / ton.

[0026] 3. The process of the present invention is simple: the preparation process involves conventional raw material soaking, composite biological enzymolysis agent, nutritional fortification, etc. The method is simple and practical, and the technical essentials can be mastered by ordinary growers, which is conducive to promotion and application;

[0027] 4. The invention has definite efficacy: it promotes crop photosynthesis and respiration, can effectively cope with low temperature and low light weather, improves crop growth phenotype and promotes crop root development and photosynthesis;

[0028] 5. The invention has significant benefits: preventing crop physiological disorders, fertilizer damage, pesticide damage, etc., improving crop resistance, reducing fertilizer and pesticide use, promoting green development in rural areas, and achieving economic, social, and ecological benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments, and the above and / or other advantages of the present invention will become more clear.

[0030] Figure 1 It is a liquid fertilizer obtained after being soaked in deionized water and a green liquid fertilizer obtained after being treated with a composite bio-enzyme preparation.

[0031] Figure 2 The growth phenotypes of tomatoes under different fertilizer treatments.

[0032] Figure 3 These are the proline and soluble sugar indicators in tomatoes under different fertilizer treatments, where a is the proline content and b is the soluble sugar content.

[0033] Figure 4 Soluble protein and root activity indicators in tomatoes under different fertilizer treatments, where a is the soluble protein content and b is the root activity.

[0034] Figure 5 This is a diagram of tomato root development under different fertilizer treatments. DETAILED DESCRIPTION

[0035] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Without departing from the spirit and essence of the present invention, modifications or substitutions made to the methods, steps or conditions of the present invention all belong to the scope of protection of the present invention. Unless otherwise specified, the experimental materials, reagents, instruments, etc. used in the present examples are all commercially available; unless otherwise specified, all technical means in the examples of the present invention are conventional means well known to those skilled in the art.

[0036] The following are the sources of raw materials for the embodiments of the present invention and the comparative examples:

[0037] Pig manure fertilizer fermented organic fertilizer: Hebei Devoduo Fertilizer Co., Ltd. (DWD / Devoduo Fertilizer, trade name is horticultural fermented pig manure fertilizer, organic fertilizer), organic matter content ≥ 25%, 5 yuan / kg;

[0038] Plant material commercial organic fertilizer: Hebei Devoduo Fertilizer Co., Ltd., processed from biological materials and plant residues, rich in organic matter;

[0039] Composite bio-enzyme preparation (Xinmulu, production license number: Jishitian (2022) H07019, implementation standard number: Q / CXDD09): Cangzhou Xindadi Biotechnology Co., Ltd., including Bacillus subtilis (viable count ≥ 2.5 billion CFU / g), Bacillus licheniformis (viable count ≥ 2.5 billion CFU / g), protease (enzyme activity ≥ 2500U / g), cellulase (enzyme activity ≥ 1000U / g) and phytase (enzyme activity ≥ 500U / g), carrier: yeast culture, stone powder;

[0040] Cellulase preparation: Shandong Longkote Enzyme Preparation Co., Ltd., enzyme activity ≥ 100,000 U / g;

[0041] Quartz sand: Tianjin Huasheng Chemical Reagent Co., Ltd., SiO2 ≥ 99.0%;

[0042] FeSO4: Tianjin Juhengda Chemical Co., Ltd., FeSO4 ≥ 99.0%;

[0043] MnSO4: Tianjin Juhengda Chemical Co., Ltd., MnSO4 ≥ 99.0%;

[0044] ZnSO4: Tianjin Juhengda Chemical Co., Ltd., ZnSO4 ≥ 99.0%;

[0045] H3BO3: Tianjin Juhengda Chemical Co., Ltd., H3BO3 ≥ 99.0%;

[0046] Citric acid: Tianmao Baoding laboratory reagent, C6H8O7·H2O;

[0047] Glutamic acid: Tianjin Huasheng Chemical Reagent Co., Ltd., content not less than 99.0%;

[0048] Arginine: Tianjin Huasheng Chemical Reagent Co., Ltd., content not less than 99.0%;

[0049] Aspartic acid: Tianjin Huasheng Chemical Reagent Co., Ltd., content not less than 99.0%;

[0050] Choline chloride: Tianjin Huasheng Chemical Reagent Co., Ltd., content is 98.0-101.0% based on chlorine.

[0051] The present invention is further explained below by specific examples:

[0052] Example 1

[0053] A method for preparing liquid fertilizer for low temperature and low light weather, the specific implementation steps are as follows:

[0054] (1) Purchase of commercial organic fertilizers and compound bio-enzyme preparations:

[0055] Purchase commercial organic fertilizers fermented with pig manure as the main raw material (pig manure fertilizer fermented organic fertilizer), which meet the national organic fertilizer standards (NY / T525-2021). Organic fertilizers have a uniform appearance, are powdery or granular, have no odor, and are measured visually and by nose. The pH of organic fertilizers is between 5.5 and 7.5, and EC ≤ 2.0dS / m;

[0056] Purchase commercial agricultural compound bio-enzyme preparations containing Bacillus subtilis, Bacillus licheniformis, protease, phytase, cellulase and other ingredients;

[0057] (2) Fertilizer soaking:

[0058] The organic fertilizer meeting the requirements in step (1) is soaked in deionized water (or pure water), the volume ratio (v:v) of deionized water (or pure water) to organic fertilizer is 3:1, 0.1wt% of quartz sand (or ordinary sand) powder (passed through a 200-mesh sieve) is added, and the mixture is continuously stirred and soaked for 30 minutes to obtain a fertilizer liquid;

[0059] (3) Composite bio-enzymatic hydrolysis:

[0060] Add a composite biological enzyme preparation to the fertilizer liquid prepared in step (2) for enzymolysis, wherein the composite biological enzyme is 1.0% (m / m) of the mass of the commercial organic fertilizer, and react for 24 hours after sufficient stirring to obtain a mixed fertilizer liquid. During the enzymolysis process, stir once every 6 hours, and stir for 15 minutes each time;

[0061] (4) Nutritional fortification:

[0062] Add a nutrient enhancer to the mixed fertilizer liquid prepared in step (3), the components of the nutrient enhancer and the concentration of each component in the mixed liquid fertilizer are: FeSO4 (50 mg / L), MnSO4 (25 mg / L), ZnSO4 (10 mg / L), H3BO3 (2 mg / L), citric acid solution (3 g / L), glutamic acid (100 mg / L), arginine (100 mg / L), aspartic acid (100 mg / L), choline chloride (1 g / L). Stir continuously for 30 minutes to obtain a liquid fertilizer stock solution, and let it stand for use;

[0063] (5) Organic green liquid fertilizer acquisition:

[0064] The liquid fertilizer stock solution prepared in step (4) is allowed to stand, and the supernatant is diluted 200-500 times and then used for soil spraying, crop drip irrigation, foliar spraying, etc. Figure 1 Shown

[0065] Example 2

[0066] This embodiment provides a method for preparing liquid fertilizer for low temperature and low light weather. The specific implementation steps are similar to those of Example 1, with the only difference being that the amount of the composite bio-enzyme preparation added in step (3) composite bio-enzyme hydrolysis is 0.1% (m / m) of the mass of the commercial organic fertilizer.

[0067] Example 3

[0068] This embodiment provides a method for preparing liquid fertilizer for low temperature and low light weather. The specific implementation steps are similar to those of Example 1, with the only difference being that the amount of the composite bio-enzyme preparation added in step (3) composite bio-enzyme hydrolysis is 0.01% (m / m) of the mass of the commercial organic fertilizer.

[0069] Example 4

[0070] This embodiment provides a method for preparing liquid fertilizer for low temperature and low light weather. The specific implementation steps are similar to those of Example 1, with the only difference being that the amount of the composite bio-enzyme preparation added in step (3) composite bio-enzyme hydrolysis is 10.0% (m / m) of the mass of the commercial organic fertilizer.

[0071] Comparative Example 1

[0072] This comparative example provides a method for preparing liquid fertilizer for low temperature and low light weather. The specific implementation steps are similar to those of Example 1, with the only difference being that the composite biological enzyme preparation added in the composite biological enzymatic hydrolysis in step (3) is replaced with deionized water of equal mass, i.e., the enzymatic hydrolysis step is not performed.

[0073] Comparative Example 2

[0074] This comparative example provides a method for preparing liquid fertilizer for coping with low temperature and low light weather. The specific implementation steps are similar to those of Example 1, the only difference being that the pig manure organic fertilizer used in step (1) is replaced with chicken manure raw material organic fertilizer (i.e., plant material commercial organic fertilizer).

[0075] Comparative Example 3

[0076] This comparative example provides a method for preparing liquid fertilizer for coping with low temperature and low light weather. The specific implementation steps are similar to those of Example 1, with the only difference being that the composite biological enzyme preparation added in the composite biological enzymatic hydrolysis in step (3) is replaced with a cellulase preparation of equal quality.

[0077] Comparative Example 4

[0078] This comparative example provides a method for preparing liquid fertilizer for coping with low temperature and low light weather. The specific implementation steps are similar to those of Example 1, with the only difference being that the composite biological enzyme preparation added in the composite biological enzymatic hydrolysis in step (3) is replaced with a protease preparation of equal quality.

[0079] Comparative Example 5

[0080] This comparative example provides a method for preparing liquid fertilizer for coping with low temperature and low light weather. The specific implementation steps are similar to those of Example 1, with the only difference being that the composite biological enzyme preparation added in the composite biological enzymatic hydrolysis in step (3) is replaced with an α-amylase preparation of equal quality.

[0081] Comparative Example 6

[0082] This comparative example provides a method for preparing a liquid fertilizer for coping with low temperature and low light weather. The specific implementation steps are similar to those of Example 1, except that the nutrient enhancer added in the nutrient fortification in step (4) is replaced by Hoagland nutrient solution, which is the most commonly used formula in plant nutrient solution. The components and their added concentrations in the mixed liquid fertilizer are 945 mg / L calcium nitrate, 607 mg / L potassium nitrate, 115 mg / L ammonium phosphate, 493 mg / L magnesium sulfate, 2.5 ml / L iron salt solution, 5 ml / L trace elements, and pH = 6.0.

[0083] The liquid fertilizer prepared in the above-mentioned Examples 1 to 4 and Comparative Examples 1 to 6 was used in a tomato pot experiment, and a blank group (no watering) was set as follows:

[0084] 1. Type of plant grown: Dwarf tomato

[0085] 2. Experimental materials and methods: In the present invention, the experiment was carried out in a greenhouse, the temperature was controlled at 10°C ± 2°C, and the light intensity was 3000-3450 LX. The liquid fertilizer used was prepared by Examples 1-4 and Comparative Examples 1-6.

[0086] 3. Experimental design: The experiment was conducted in a temperature-light controlled greenhouse of the College of Resources and Environmental Sciences of Nanjing Agricultural University. The temperature was controlled at 10℃±5℃ and the light intensity was 3000~3450LX. The experiment was conducted from November 2023 to December 2023. A universal matrix was selected to arrange the tomato pot experiment.

[0087] The experiment set up multiple treatments, including a blank group (no fertilization measures), and liquid fertilizer treatments with 200-fold dilution of liquid fertilizer in Examples 1 to 4 and Comparative Examples 1 to 6, and each treatment was repeated three times. During the test period, liquid fertilizer was applied once for root irrigation at the three-leaf stage of tomato seedlings, and leaves were sprayed once at the flower bud differentiation stage (5-6 leaf stage). On the 7th day after leaf spraying, the functional plant height, biomass, chlorophyll content, leaf N content, leaf proline content, leaf soluble protein, soluble sugar content and root activity of the plants were measured. The growth phenotypes of tomatoes obtained by different liquid fertilizer treatments are as follows: Figure 2 After the experiment, the physiological and biochemical indexes of tomatoes were measured, and the experimental data were analyzed by one-way variance analysis, and the differences among the treatments were extremely significant.

[0088] 4. Analytical methods

[0089] (1) Leaf chlorophyll SPAD value: Use a chlorophyll meter to measure the leaf chlorophyll SPAD value;

[0090] (2) Leaf N content: The leaf N content was measured using a chlorophyll meter;

[0091] (3) Biomass: Cut the aboveground and underground parts of the plants, rinse them with deionized water, wipe them dry and measure the fresh weight;

[0092] (4) Root morphology analysis: After the roots were rinsed with deionized water, the images were analyzed using WinRHIZO root analysis software to determine the plant root index parameters;

[0093] (5) Proline: determined by sulfosalicylic acid method;

[0094] (6) Soluble sugar: determined by anthrone colorimetry;

[0095] (7) Soluble protein: determined by BCA protein method;

[0096] (8) Root activity: measured by TTC (triphenyltetrazolium chloride) method.

[0097] 5. The application experiment results are as follows:

[0098] (1) Optimization experiment of the dosage of composite biological enzymatic hydrolyzer

[0099] In the preliminary experiment, the effect of different addition amounts of composite biological enzyme preparations (0.01%, 0.1%, 1.0%, 10.0%, Examples 1 to 4) on the hydrolysis efficiency of organic fertilizer was tested. By measuring the soluble sugar, free amino acid content and molecular weight distribution of enzymatic hydrolysis products, it was found that: when the amount of enzymatic hydrolyzer was ≤0.1%, the hydrolysis was insufficient, the product was mainly small molecule oligosaccharides, and the amino acid release was insufficient; when the amount of enzymatic hydrolyzer was ≥5.0%, the reaction was too violent, a large amount of insoluble precipitates were generated, and the fertilizer efficiency was reduced; the addition amount of 1.0% was the most easily absorbed by crops, which was significantly better than other proportions. Therefore, the present invention selects 1.0% as the optimal amount of enzymatic hydrolyzer, balancing cost and effect.

[0100] (2) Verification of the irreplaceability of key components

[0101] 1) Effects on tomato growth phenotype

[0102] Table 1 Tomato growth phenotypic indicators

[0103]

[0104] Compared with the blank group, the liquid fertilizer prepared by the preparation method described in Example 1 significantly increased the plant height, aboveground fresh weight and root fresh weight of tomatoes, reaching 10.60 cm, 3.50 g·plant-1 and 0.57 g·plant-1 respectively (P<0.05). Comparative Examples 1-6 affected plant growth to varying degrees, among which the plant height (7.92 cm) of Comparative Example 6 was higher, but the aboveground fresh weight (0.66 g·plant-1) was the lowest, while the aboveground fresh weight (1.16 g·plant-1) of Comparative Example 3 was higher than that of the other comparative examples, indicating that Example 1 had a positive and significant effect on the growth phenotype of tomatoes.

[0105] 2) Effects on tomato stress resistance and photosynthesis indicators

[0106] Table 2 Physiological and biochemical indicators of tomato

[0107]

[0108] As shown in Table 2, Figure 3 and Figure 4As shown, the chlorophyll content, N content, proline content, soluble sugar, soluble protein and root activity of tomato plants using the liquid fertilizer prepared in Example 1 are all higher than those in Comparative Examples 1-6 and the blank group (P<0.05), indicating that the green liquid fertilizer prepared in Example 1 can enhance the ability of tomatoes to cope with low temperature and low light. Tomatoes applied with the liquid fertilizer of Example 1 can generate more osmotic regulators such as proline and soluble sugar to resist damage from adverse conditions, and soluble proteins can use their hydrophilicity to reduce cell osmotic potential and participate in metabolism in the plant. Root activity is an important factor reflecting the vitality of the root system. Strong root activity can accelerate plant metabolism, enhance root absorption performance, ensure that plants absorb water and transport substances, and improve plant stress resistance.

[0109] 3) Effects on tomato root development

[0110] Table 3 Tomato root development morphology

[0111]

[0112]

[0113] From Table 3 and Figure 5 It can be seen that the application of the liquid fertilizer prepared in Example 1 is beneficial to the root development of tomato plants. The total root length, surface area, total root volume, average root diameter, number of root tips and number of branches of the tomato plants are significantly increased compared with the comparative examples 1-6 and the blank control (P<0.05), which shows that the application of the organic water-soluble fertilizer in Example 1 can promote the root development of tomato plants and is beneficial for plants to absorb nutrients from the soil.

[0114] It can be seen from the above experimental data that the present invention explores the irreplaceability of key components in the liquid organic fertilizer prepared by the present invention by replacing pig manure organic fertilizer (replaced with chicken manure), replacing composite biological enzyme preparations (replaced with only cellulase, protease or alpha amylase), and replacing trace elements (Fe / Mn / Zn / B) and amino acids. The pig manure organic fertilizer, composite biological enzyme preparations, trace elements and amino acids in the liquid organic fertilizer prepared by the present invention are key components for synergistic enhancement, and none of them can be missing.

[0115] The present invention provides a method for preparing a green liquid fertilizer and a method for using the fertilizer in resisting low temperature and low light stress. There are many methods and ways to implement the technical solution. The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention. All components not specified in this embodiment can be implemented by existing technologies.

Claims

1. A method for preparing green liquid fertilizer, characterized in that: The steps include: (1) mixing pig manure organic fertilizer and solvent, adding sand, and obtaining fertilizer liquid; (2) mixing the fertilizer liquid with a composite biological enzyme preparation and obtaining a mixed fertilizer liquid by enzymolysis; (3) Add FeSO4, MnSO4, ZnSO4, H3BO3, citric acid, glutamic acid, arginine, aspartic acid and choline chloride to the mixed fertilizer liquid for nutrient enhancement, mix well and let stand, and the supernatant is the green liquid fertilizer.

2. The preparation method according to claim 1, characterized in that: In step (1), the pig manure organic fertilizer is commercially available pig manure organic fertilizer, has a pH of 5.5 to 7.5, and an electrical conductivity of ≤2.0dS / m.

3. The preparation method according to claim 1, characterized in that: In step (1), the solvent is a solvent with a conductivity of less than 0.1 dS / m, including any one of deionized water, pure water or rainwater.

4. The preparation method according to claim 1, characterized in that: In step (1), the mixing volume ratio of the pig manure organic fertilizer and the solvent is 1 to 5:1; the sand is sand powder passed through a 200-mesh sieve, and the added concentration of the sand in the fertilizer liquid is 0.05wt% to 0.5wt%.

5. The preparation method according to claim 1, characterized in that: In step (2), the composite bio-enzyme preparation is a commercially available composite bio-enzyme preparation, which includes Bacillus subtilis ≥ 2.5 billion CFU / g, Bacillus licheniformis ≥ 2.5 billion CFU / g, protease ≥ 2500U / g, cellulase ≥ 1000U / g and phytase ≥ 500U / g.

6. The preparation method according to claim 1, characterized in that: In step (2), the added mass of the composite bio-enzyme preparation is 0.5% to 10% of the mass of the pig manure organic fertilizer in step (1); the enzymolysis reaction time is 24 hours, and the enzymolysis process is stirred once every 4 to 6 hours, and each stirring time is 10 to 20 minutes.

7. The preparation method according to claim 1, characterized in that: In step (3), the added concentration of FeSO4 is 40-60 mg / L, the added concentration of MnSO4 is 20-30 mg / L, the added concentration of ZnSO4 is 5-15 mg / L, the added concentration of H3BO3 is 1-3 mg / L, the added concentration of citric acid is 2-4 g / L, the added concentration of glutamic acid is 80-110 mg / L, the added concentration of arginine is 80-110 mg / L, the added concentration of aspartic acid is 80-110 mg / L, and the added concentration of choline chloride is 0.5-2 g / L.

8. The green liquid fertilizer prepared by the preparation method according to any one of claims 1 to 7.

9. Use of the green liquid fertilizer according to claim 8 in enhancing the ability of crops to resist low temperature and low light stress, wherein the low temperature and low light have a temperature of 5 to 15°C and an illumination of 3000 to 3450 LX.

10. The method for applying green liquid fertilizer according to claim 8, characterized in that: The green liquid fertilizer is sprinkled on the soil, irrigated on the roots or sprayed on the leaves, wherein the dilution multiple of the green liquid fertilizer during application is 200 to 500 times.

Images