Application of potato residual liquid bacterial fertilizer in apple planting

Abstract

The invention discloses application of a potato residual liquid bacterial fertilizer in apple planting, and relates to the technical field of agricultural planting. The invention relates to application of a potato residual liquid bacterial fertilizer in apple planting. The potato residual liquid bacterial fertilizer is prepared by the following steps: 1, separating archaea for more than one hundred years in an extreme environment; 2, purifying the archaea; 3, rejuvenating the archaea; 4, extracting the slave fungus from the roots of robust fruit trees such as apples and strawberries, then separating various slave fungus strains, determining through strain gene detection, and then classifying, purifying and rejuvenating; 5, archaea is cultivated on a PDA nutrient medium to form mother bacteria, then the mother bacteria are implanted into a proper culture medium for secondary propagation, and third propagation is conducted in the same step to obtain strains for standby application. Bacterial manure generated by fermenting potato residual liquid through the flora can effectively improve the quality of fruits such as apples and strawberries, balance the sweet-to-acid ratio and improve the taste, and the yield of the fruits is improved. The yield and the soil are improved. Meanwhile, the fertilizer utilization rate can be increased, the chemical fertilizer usage amount is reduced, and weight-losing and efficiency-increasing are

Description

Technical Field

[0001] The invention relates to the technical field of agricultural planting, and in particular to application of potato residual liquid bacterial fertilizer in apple planting. Background Art

[0002] Bacterial fertilizer, also known as biological fertilizer, biofertilizer, bacterial fertilizer or inoculant, etc. Bacterial fertilizer, figuratively speaking, is "bacteria + fertilizer", because bacterial fertilizer contains "organic matter, nitrogen, phosphorus, potassium" and other nutrients necessary for crop growth, and also contains a large number of beneficial microorganisms, which reproduce in the soil and play a role in improving soil and preventing diseases. Potato residue is the waste liquid produced by the production of potato starch. At present, most of it is directly discharged, and a small part is used as fertilizer after simple fermentation.

[0003] In the process of realizing the present invention, the inventors found that the prior art has the following problems: most of the current potato residual liquid is directly discharged, and a small part is simply fermented and used as fertilizer, but this method cannot effectively improve the quality of the planted products and has poor effectiveness. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides an application of potato residual liquid bacterial fertilizer in apple planting, which solves the problem that most of the existing potato residual liquid is directly discharged, and a small part is used as fertilizer after simple fermentation. However, this method cannot effectively improve the quality of the planted products and has poor effectiveness.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: an application of potato residual liquid bacterial fertilizer in apple planting requires the following steps to complete the preparation: 1. Separating archaea over 100 years old in extreme environments; 2. Purifying archaea; 3. Rejuvenating archaea; 4. Extracting branch fungi from the roots of healthy fruit trees, such as apples and strawberries, and then separating various branch fungi strains, determining them through strain gene detection, and then classifying, purifying, and rejuvenating; 5. Cultivating archaea into mother bacteria on PDA nutrient medium, and then implanting the mother bacteria on a suitable culture medium for secondary cultivation. 1. Expand and propagate, and the strains obtained by carrying out the same steps three times are set aside; 2. Cultivate the slave fungus into mother bacteria on the PDA nutrient medium, and then implant the mother bacteria on a suitable culture medium for a second expansion, and the strains obtained by carrying out the same steps three times are set aside; 3. Implant Bacillus subtilis, Bacillus licheniformis and other strains on a suitable culture medium for a second expansion, and the strains obtained by carrying out the same steps three times are set aside; 4. Calculate the metabolite ratio of various prepared strains, infer the strain preparation ratio, and then prepare the composite bacterial community according to the ratio, and then ferment until it is stable.

[0006] Preferably, the content of each bacterial species is tested to see if it meets the required ratio. If not, adjustments are made until it meets the requirements.

[0007] Preferably, the potato residue is added to the starter and fermented until mature.

[0008] Preferably, the prepared composite bacterial flora is implanted into the potato residual liquid and can be used after being tested and found to be qualified.

[0009] Beneficial Effects

[0010] The present invention provides an application of potato residual liquid bacterial fertilizer in apple planting. The bacterial fertilizer generated by fermenting potato residual liquid with the bacterial community can effectively improve the quality of fruits such as apples and strawberries, balance the sweet-acid ratio, improve the taste, increase the yield, and improve the soil. At the same time, it can improve the fertilizer utilization rate, reduce the amount of chemical fertilizer used, and achieve weight loss and efficiency improvement. DETAILED DESCRIPTION

[0011] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0012] The present invention provides a technical solution: an application of potato residual liquid bacterial fertilizer in apple planting, which needs to be prepared by the following steps: 1. isolating archaea over 100 years old in an extreme environment; 2. purifying the archaea; 3. rejuvenating the archaea; 4. extracting branch fungi from the roots of healthy fruit trees, such as apples, strawberries, etc., and then isolating various branch fungi strains, determining them through strain gene detection, and then classifying, purifying, and rejuvenating; 5. cultivating the archaea into mother bacteria on a PDA nutrient medium, and then implanting the mother bacteria on a suitable culture medium for secondary propagation, and the same steps are repeated. 6. Cultivate the strain obtained by three expansions on the PDA nutrient medium from the slave fungus into mother bacteria, then implant the mother bacteria on a suitable culture medium for a second expansion, and perform the same steps for three expansions to obtain the strains to be used; 7. Implant Bacillus subtilis, Bacillus licheniformis and other strains on a suitable culture medium for a second expansion, and perform the same steps for three expansions to obtain the strains to be used; 8. Calculate the metabolite ratio of various prepared strains, infer the strain preparation ratio, and then prepare a composite bacterial community according to the ratio, and then ferment until it is stable.

[0013] Preferably, the content of each bacterial species is tested to see if it meets the required ratio. If not, adjustments are made until it meets the requirements.

[0014] Preferably, the potato residue is added to the starter and fermented until mature.

[0015] This embodiment is further configured to implant the prepared composite bacterial flora into the potato residual liquid, and use the potato liquid after passing the test.

[0016] The detailed connection means are well-known in the art. The following mainly introduces the working principle and process. The specific work is as follows.

[0017] Embodiment: Archaea over 100 years old are isolated from extreme environments, the archaea are purified, the archaea are rejuvenated, the fungi are extracted from the roots of healthy fruit trees, such as apples and strawberries, and then various fungi strains are isolated and confirmed by strain gene detection. Then the archaea are classified, purified, and rejuvenated. The archaea are cultivated into mother bacteria on a PDA nutrient medium, and the mother bacteria are then implanted on a suitable culture medium for secondary propagation. The strains obtained by the same steps are repeated three times for future use. The fungi are cultivated into mother bacteria on a PDA nutrient medium, and the mother bacteria are then implanted on a suitable culture medium for secondary propagation. The strains obtained by the same steps are repeated three times for future use. Bacillus subtilis, Bacillus licheniformis and other strains are implanted on a suitable culture medium for secondary propagation. The strains obtained by the same steps are repeated three times for future use. Species are prepared for use, the metabolite ratio of various strains is calculated, the strain preparation ratio is deduced, and then a composite bacterial community is prepared according to the ratio, and then fermented until it is stable, and the content of each strain is tested to see if it meets the required ratio. If it does not meet the requirements, it is adjusted until it meets the requirements, and the potato residue is added to the fermentation agent for fermentation until it matures, and the prepared composite bacterial community is implanted into the potato residue. It can be used after the test is qualified. The bacterial fertilizer can be used as a base fertilizer root irrigation, and can be used as a foliar fertilizer spray to improve the taste, increase yield, and improve the soil. At the same time, it can improve the fertilizer utilization rate, reduce the use of chemical fertilizers, and achieve the effects of weight loss and efficiency.

[0018] It should be noted that, in this document, relational terms such as first and second, etc. are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations.

Claims

1. An application of potato residual liquid bacterial fertilizer in apple planting, characterized in that: The following steps are required to complete the preparation:

1. Isolate archaea that are more than 100 years old in extreme environments; 2. Purify the archaea; 3. Rejuvenate the archaea; 4. Extract the fungus from the roots of healthy fruit trees, such as apples and strawberries, and then separate various fungus strains, identify them through strain gene testing, and then classify, purify and rejuvenate them; 5. Cultivate Archaea into mother bacteria on PDA nutrient medium, then implant the mother bacteria into a suitable culture medium for secondary propagation, and repeat the same steps for three times to obtain the strains for future use; 6. Cultivate Mycobacteria into mother bacteria on PDA nutrient medium, then implant the mother bacteria into a suitable culture medium for secondary propagation, and repeat the same steps for three times to obtain the strains for future use; 7. Implant Bacillus subtilis, Bacillus licheniformis and other strains into a suitable culture medium for secondary propagation, and repeat the same steps for three times to obtain the strains for future use; 8. Calculate the metabolite ratio of various prepared strains, infer the strain preparation ratio, and then prepare the composite bacterial community according to the ratio, and then ferment until it is stable.

2. The application of potato residual liquid fertilizer in apple planting according to claim 1 is characterized in that: Check whether the content of each bacterial strain meets the required ratio. If not, make adjustments until it meets the requirements.

3. The application of potato residual liquid fertilizer in apple planting according to claim 1 is characterized in that: The potato residue is added to the starter and fermented until mature.

4. The application of potato residual liquid fertilizer in apple planting according to claim 1 is characterized in that: Implant the prepared composite bacterial flora into the potato residual liquid and use it after passing the test.