Composite preparation based on rhamnolipid and PHBV bioactive preparation and method
By using a composite preparation of rhamnolipid and PHBV bioactive preparation in saline soil, the problem of improving saline soil and increasing oil sunflower yield in the prior art has been solved, and the soil improvement and crop growth have been significantly improved, while reducing costs and environmental impacts.
Patent Information
- Application Number
- CN202510150757.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-11
- Publication Date
- 2025-05-13
AI Technical Summary
The prior art is difficult to effectively improve saline-alkali soil, especially to increase the yield of sunflower crops in alkaline soil, and the traditional methods are costly and have a great environmental impact.
A composite preparation based on rhamnolipid and PHBV bioactive preparation is used to form a composite preparation by dissolving it in water and mixing it evenly, and watering it in saline-alkali soil to improve soil physicochemical properties and promote plant growth.
It significantly improves the growth status and yield of the oil sunflower crop, improves the stress resistance of the crop, enhances the soil's water and fertilizer retention ability, reduces salt concentration, and reduces environmental pollution.
Smart Images
Figure CN119979173A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the field of salinized soil or alkaline soil improvement, and specifically relates to a composite preparation based on rhamnolipid and PHBV bioactive preparation, a preparation method and a method for improving the growth of oil sunflower crops in saline-alkali soil. Background Art
[0002] Salt-alkali land is a type of obstructive land where excessive salt enrichment leads to soil degradation. It is a general term for soils with a salt content of more than 0.1% and an alkalinity of more than 15% and less than 20%, including saline soil and alkaline soil. According to a 2021 report by the Food and Agriculture Organization of the United Nations, due to drought, seawater erosion and other factors, soils in more than 100 countries are plagued by salinization problems, with an estimated area of about 1 billion hectares, accounting for about 8.7% of the world's land area. About 1 / 5 of the world's cultivated land and 1 / 3 of the irrigated land are affected by soil salinization, mainly distributed in arid and semi-arid coastal areas of various climatic zones in the world. It is estimated that the area of saline-alkali soil is growing at a rate of 1%-2% per year. Soil salinization seriously restricts the sustainable use of water and soil resources and the increase in crop yields (such as sunflower and rapeseed).
[0003] Improving and utilizing salinized soil can not only alleviate the arable land crisis, increase crop yields and promote food security, but also improve the agricultural ecological environment and improve people's quality of life. Researchers have conducted a lot of research on the physical and chemical properties and structure of saline-alkali soil, the structure and function of microorganisms, and the growth effects of plants, and have put forward targeted soil improvement measures according to different saline-alkali soils. At present, saline-alkali land improvement technologies can be divided into physical improvement, chemical improvement, biological improvement and engineering improvement. However, physical methods require a lot of manpower and high implementation costs, and biological methods are very time-consuming and often limited by environmental factors. In addition, some modern technologies and reclamation methods for saline-alkali soils, such as genetic and electrical improvement, are limited by their high cost and low efficiency. In contrast, chemical methods are considered to be the most effective and widely used methods to improve saline-alkali soils, but they can often only change a certain property of the soil. Therefore, the combination of amendments has become a potential strategy to effectively alleviate salt stress in saline-alkali soils.
[0004] At the same time, traditional soil improvement methods mostly rely on chemical improvers or mechanical operations, which are often costly and may affect the soil microenvironment. In the prior art, rhamnolipids have been used to improve soil for cotton planting, but there have been no studies on their application in alkaline soil for sunflower planting. Therefore, a more cost-effective and environmentally friendly soil improvement method is urgently needed, especially to increase the yield of sunflower in alkaline soil.
[0005] Rhamnolipid is a biosurfactant produced by microbial metabolism. It has the properties of reducing surface tension, stabilizing emulsions and foaming. Compared with chemically synthesized surfactants, it is also non-toxic and biodegradable. It is an environmentally friendly green product with good application prospects in petrochemicals, environmental remediation, biomedicine, agricultural fertilizers and food hygiene.
[0006] PHBV bioactive preparation is a fermentation liquid made by neutralizing the remaining liquid to neutral or acidic after using phosphoric acid to produce PHBV biodegradable materials (i.e. PHBV bioactive preparations) using starch or glucose as raw materials and fermentation engineering technology. PHBV bioactive preparations are fermentation liquids produced during the production process of PHBV. The PHBV content is about 4.66g / L. It is estimated that about 30 tons of fermentation liquid are produced for every ton of PHBV produced. In addition, PHBV can be produced by microorganisms under conditions rich in N and P. Therefore, the fermentation liquid contains a large amount of nutrients such as C, N, and P, which can be used to improve poor soils and provide nutrients for the growth and development of crops and soil microorganisms. Summary of the invention
[0007] The purpose of the present invention is to overcome the defects in the prior art and provide a composite preparation and method based on rhamnolipid and PHBV bioactive preparation.
[0008] The specific technical solutions adopted by the present invention are as follows:
[0009] In a first aspect, the present invention provides a method for preparing a composite preparation based on rhamnolipid and PHBV bioactive agent, as follows:
[0010] The rhamnolipid and the PHBV bioactive preparation are dissolved in water and mixed evenly to obtain a composite preparation.
[0011] Preferably, the mass fraction of the rhamnolipid is 6%.
[0012] Preferably, the PHBV bioactive preparation is neutralized with phosphoric acid in advance to a pH between 6.5 and 7.3.
[0013] Preferably, in the composite preparation, 8.3 ml of rhamnolipid and 11.5 ml of PHBV bioactive preparation are added to every 1 L of water.
[0014] In a second aspect, the present invention provides a composite preparation based on rhamnolipid and PHBV biologically active agent obtained by any preparation method described in the first aspect.
[0015] In a third aspect, the present invention provides a method for improving the growth of oil sunflower crops in saline-alkali soil, as follows:
[0016] Before planting crops, the composite preparation based on rhamnolipid and PHBV bioactive agent as described in the second aspect is irrigated into the saline-alkali soil where oil sunflower crops are to be planted; under the synergistic effect of rhamnolipid and PHBV bioactive agent, the physical and chemical properties of saline-alkali soil can be improved, plant growth and development can be promoted, and the stress resistance of oil sunflower crops can be improved.
[0017] Preferably, the application amount of the composite preparation is 60 to 80 L / mu.
[0018] Compared with the prior art, the present invention has the following beneficial effects:
[0019] The present invention significantly improves the growth and yield of oil sunflower by applying a composite preparation of rhamnolipid and PHBV bioactive agent in alkaline soil through synergistic effect. Rhamnolipid improves the structure and physical properties of the soil by combining with harmful sodium ions in the soil, thereby improving the water and nutrient supply capacity of the crop root zone. The present invention can not only be applied through traditional irrigation methods, but also can be integrated into a drip irrigation system to achieve timed and quantitative application, thereby achieving the purpose of accurately improving the soil and saving costs. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 The height changes of oil sunflower plants at different stages;
[0021] Figure 2 The diameter changes of oil sunflower plants at different stages;
[0022] Figure 3 The changes of chlorophyll content in different stages of oil sunflower plants;
[0023] Figure 4 The fresh weight (a), dry weight (b), number of leaves (c), and disk diameter (d) of oil sunflower;
[0024] Figure 5 Total seed weight (a) and 100-seed weight (b) of oil sunflower seeds. DETAILED DESCRIPTION
[0025] The present invention is further described and illustrated below in conjunction with the accompanying drawings and specific embodiments. The technical features of each embodiment of the present invention can be combined accordingly without conflicting with each other.
[0026] The present invention provides a method for preparing a composite preparation based on rhamnolipid and PHBV biologically active preparation, and the preparation method is specifically as follows:
[0027] The rhamnolipid and the PHBV bioactive preparation are dissolved in water and the two are mixed evenly to obtain a composite preparation.
[0028] As a preferred embodiment of the present invention, the mass fraction of rhamnolipid is 6%; the PHBV bioactive preparation is neutralized with phosphoric acid in advance to make the pH value between 6.5 and 7.3.
[0029] Specifically, the PHBV bioactive preparation was purchased from Ningbo Tianan Biomaterial Co., Ltd. It is a PHBV fermentation broth produced by RALSTONIA ENTROPHA under appropriate fermentation conditions using glucose as a raw material. Rhamnolipids were prepared by referring to the commonly used fermentation method in the prior art.
[0030] As a preferred embodiment of the present invention, in the composite preparation, 8.3 ml of rhamnolipid and 11.5 ml of PHBV bioactive preparation are added to every 1 L of water.
[0031] The composite preparation prepared by the present invention can be irrigated in saline-alkali soil where oil sunflower crops need to be planted; under the synergistic effect of rhamnolipid and PHBV biologically active preparation, the physical and chemical properties of saline-alkali soil can be improved, plant growth and development can be promoted, and the stress resistance of oil sunflower crops can be improved.
[0032] Specifically, the compound preparation can improve the soil environment and promote plant growth in the following ways:
[0033] 1) Improve soil physical and chemical properties: Rhamnolipids and PHBV bioactive agents can significantly improve the physical structure of saline-alkali soil, enhance the soil's ability to retain water and fertilizer, reduce salt concentration, and improve the root environment, thereby providing better conditions for plant growth.
[0034] 2) Promote plant growth and development: Through the combination of rhamnolipid and PHBV bioactive agents, the compound preparation can significantly increase the plant height, fresh weight, dry weight, disk diameter and grain yield of oil sunflower crops. Rhamnolipid, as a surfactant, can enhance the absorption of nutrients by oil sunflower crops and stimulate the development of the root system of oil sunflower crops; while PHBV bioactive agents can slowly release nutrients and provide long-term and effective support for the growth of oil sunflower crops.
[0035] 3) Improve plant stress resistance: The compound preparation can enhance the stress resistance of oil sunflower crops in saline-alkali environments, so that oil sunflower crops can still maintain a good growth trend under high saline-alkali conditions and reduce growth stagnation or yield reduction caused by saline-alkali stress.
[0036] As a preferred embodiment of the present invention, the application amount of the composite preparation is 60 to 80 L / mu.
[0037] The composite preparation provided by the present invention not only exhibits excellent performance in soil improvement, but also significantly improves the growth rate and seed yield of oil sunflower by improving the alkaline soil environment and crop root zone conditions. In particular, the combined use of rhamnolipid and PHBV bioactive preparation further optimizes the soil improvement effect. Therefore, the present invention provides an efficient and environmentally friendly solution for saline-alkali land improvement and oil sunflower crop planting.
[0038] This technical solution not only has significant application value in improving saline-alkali soil, but can also be applied to other farming systems that require improved soil environment, and has broad promotion prospects.
[0039] The present invention will be further described below in conjunction with the embodiments.
[0040] Example 1
[0041] In this embodiment, a composite preparation based on rhamnolipid and PHBV biologically active agent is prepared. The preparation method of the composite preparation is as follows:
[0042] 8.3 ml of rhamnolipid (mass fraction 6%) and 411.5 ml of PHBV bioactive preparation neutralized with phosphoric acid were dissolved in 1 L of water, and stirred and mixed evenly with a glass rod to obtain a composite preparation.
[0043] Example 2
[0044] The soil collected from the southern rural area of Dingji Town, Huaiyin District, Huai'an City, Jiangsu Province (33.67695°N, 118.97245°E) was air-dried, and the impurities such as roots and stones visible to the naked eye were removed, and the soil was homogenized after grinding through a 2mm sieve. The experiment was 3 levels and 4 replicates. After adding 2kg of soil to each flower pot, the corresponding amount of PHBV bioactive preparation and rhamnolipid were added to the container, respectively, and recorded as WCK, HSF, and 40HSF (as shown in Table 1). The water was kept at 60% of the maximum water holding capacity, and the soil was pre-cultured for 1 week to activate the original microorganisms in the soil. The oil sunflower seeds were disinfected with hydrogen peroxide for 30 minutes, sown after germination, the light intensity was 12000lux, the light duration was 10h / day, and watering was weighed every day. The culture period was 80 days. During the seedling stage, 100ml of Hogland nutrient solution was poured every week. After the buds appeared, 200ml of Hogland nutrient solution was changed to meet the nutrients required for plant growth.
[0045] Table 1 Specific experimental plan
[0046]
[0047] 1. During the experiment, the plant height was measured with a tape measure, the plant diameter with a vernier caliper, and the chlorophyll content was measured with a chlorophyll meter. The measurements were carried out at the seedling stage (20 days), bud stage (40 days), flowering stage (60 days), and fruiting stage (80 days) of the oil sunflower.
[0048] The results of the determination of plant height, plant diameter, and chlorophyll content of oil sunflower are as follows:
[0049] like Figure 1 and 2 As shown in the figure, during the 20, 40, 60, and 80-day growth processes, the plant height and stem diameter of the experimental groups SZF and 40SZF were increased to varying degrees compared with the control group, especially in the 40SZF group.
[0050] like Figure 3 As shown in the figure, in the first 40 days, the chlorophyll content of the treatment group 40SZF was significantly higher than that of other groups, indicating that the combined use of rhamnolipids and PHBV bioactive agents helps to improve the photosynthesis efficiency of plants. The small impact on the later stage may be due to other environmental influences that reduce the difference. In short, rhamnolipids not only show excellent performance in soil improvement, but also significantly increase the growth rate and seed yield of sunflower by improving the alkaline soil environment and crop root zone conditions. In particular, the combined use of rhamnolipids and PHBV bioactive agents further optimizes the soil improvement effect.
[0051] 2. During the experiment, 80 days after the plants were treated, the above-ground parts were weighed; the diameter of the sunflower disk was measured with a vernier caliper; the length and width of the leaves were measured with a ruler, and the number of sunflower leaves was recorded.
[0052] The results of the determination of the fresh weight, dry weight, disk diameter, number of leaves, and leaf width-to-length ratio of oil sunflower are as follows:
[0053] like Figure 4 As shown, compared with the control group, the fresh weight, dry weight and disk diameter of the treatment group were significantly increased, the number of leaves increased, and the disk diameter in the 40SZF group was significantly larger than that in the control group, indicating that the combined use of rhamnolipid and PHBV bioactive agents had a positive effect on the development of flowering organs of oil sunflower.
[0054] 3. Cut off the flower disk of the oil sunflower, dry it to constant weight, sow the seeds in the flower disk and weigh the total weight of the seeds with a balance, and record the weight of 100 seeds.
[0055] The results of the determination of 100-grain weight and total weight of oil sunflower seeds are as follows:
[0056] like Figure 5As shown, the total seed weight of the 40SZF and SZF groups was significantly higher than that of the WCK group, with the highest yield increase of 58%. However, in terms of 100-grain weight, the 40SZF group was slightly higher than the other treatment groups, indicating that rhamnolipids have a certain promoting effect on oil sunflower yield.
[0057] The above-described embodiment is only a preferred solution of the present invention, but it is not intended to limit the present invention. A person skilled in the relevant technical field may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, any technical solution obtained by equivalent replacement or equivalent transformation falls within the protection scope of the present invention.
Claims
1. A method for preparing a composite preparation based on rhamnolipid and PHBV biologically active agent, characterized in that: The details are as follows: The rhamnolipid and the PHBV bioactive preparation are dissolved in water and mixed evenly to obtain a composite preparation.
2. The method for preparing the composite preparation based on rhamnolipid and PHBV biologically active agent according to claim 1, characterized in that: The mass fraction of the rhamnolipid is 6%.
3. The method for preparing the composite preparation based on rhamnolipid and PHBV biologically active agent according to claim 1, characterized in that: The PHBV biologically active preparation is neutralized with phosphoric acid in advance to make the pH value between 6.5 and 7.
3.
4. The method for preparing the composite preparation based on rhamnolipid and PHBV biologically active agent according to claim 1, characterized in that: In the composite preparation, 8.3 ml of rhamnolipid and 11.5 ml of PHBV bioactive preparation were added to every 1 L of water.
5. A composite preparation based on rhamnolipid and PHBV biologically active agent obtained by the preparation method according to any one of claims 1 to 4.
6. A method for improving the growth of oil sunflower crops in saline-alkali soil, characterized in that: The details are as follows: The composite preparation based on rhamnolipid and PHBV bioactive agent as claimed in claim 5 is irrigated into saline-alkali soil where oil sunflower crops need to be planted; under the synergistic effect of rhamnolipid and PHBV bioactive agent, the physical and chemical properties of saline-alkali soil can be improved, plant growth and development can be promoted, and the stress resistance of oil sunflower crops can be improved.
7. The method according to claim 6, characterized in that The application amount of the composite preparation is 60 to 80 L / mu.