Preparation method of rice wine with sleep-aiding and anti-fatigue effects

Abstract

The invention discloses a preparation method of rice wine with sleep-aiding and anti-fatigue effects, which comprises the following steps: mixing a jasmine flower root extract, a rosemary extract, anredera cordifolia and a pericarpium citri reticulatae extract to obtain a mixed extract; and uniformly mixing the mixed extract with the base wine, filling carbon dioxide, filling and sterilizing to obtain the rice wine. By utilizing the synergistic effect of the four natural plant extracts, namely the jasmine flower root extract, the rosemary extract, the anredera cordifolia extract and the pericarpium citri reticulatae extract, the feasibility of efficient and safe use is ensured, so that the blank of the existing market is filled, and a better choice is provided for people who need to relax and recover in the modern fast-paced life.

Description

Technical Field

[0001] The invention relates to the field of rice wine production, and more specifically, to a method for preparing rice wine having sleep-aiding and anti-fatigue effects. Background Art

[0002] As the pace of life in modern society accelerates, more and more people are facing sleep problems and fatigue. Long-term lack of sleep and excessive fatigue not only affect the quality of daily life, but may also lead to a variety of health problems, such as decreased immunity and cardiovascular diseases. Therefore, the market demand for products with sleep-aiding and anti-fatigue effects is increasing.

[0003] The common sleep aid and anti-fatigue products on the market mainly include:

[0004] Drugs: such as benzodiazepines Drugs such as diazepam and non-benzodiazepines Although these drugs can significantly improve sleep quality in the short term, long-term use may lead to dependence and side effects.

[0005] Natural health products: such as melatonin, L-theanine, valerian root extract, etc. These products are generally considered to be a safer choice, but their effects vary greatly from person to person, and the efficacy of some products has not been fully verified.

[0006] Although there are many sleep-aiding and anti-fatigue products on the market, the existing technologies still have the following problems:

[0007] Drug dependence: Drug-based sleep aids (such as diazepam) are effective in the short term, but long-term use may lead to dependence and addiction, and withdrawal symptoms may occur after stopping the drug. In addition, drugs may produce a series of side effects, such as daytime sleepiness and memory loss.

[0008] Large individual differences: The effects of natural health products vary greatly from person to person, and some ingredients may not be effective or have no obvious effects on some people. At the same time, due to the lack of strict clinical verification, its safety and effectiveness are difficult to guarantee.

[0009] Existing sleep-aid and anti-fatigue products have many deficiencies in terms of safety, effect stability, etc. In particular, long-term use may lead to dependence and side effects, and may also be ineffective or ineffective for some people. Therefore, the development of a safe and effective sleep-aid and anti-fatigue functional drink has become an urgent problem to be solved. Summary of the invention

[0010] The purpose of the present invention is to provide a method for preparing rice wine with sleep-inducing and anti-fatigue effects, which utilizes the synergistic effect of four natural plant extracts - jasmine root extract, rosemary extract, notoginseng extract and tangerine peel extract to ensure the feasibility of efficient and safe use, thereby filling the gap in the existing market and providing a better choice for people who need relaxation and recovery in modern fast-paced life.

[0011] In order to achieve these purposes and other advantages of the present invention, a method for preparing rice wine having sleep-aiding and anti-fatigue effects is provided, comprising:

[0012] The jasmine root extract, rosemary extract, notoginseng and tangerine peel extract are mixed to obtain a mixed extract;

[0013] The mixed extract is mixed evenly with the base wine, and then carbon dioxide is injected, filled, and sterilized to obtain rice wine.

[0014] Preferably, in the method for preparing the rice wine having sleep-aiding and anti-fatigue effects, the method for preparing the jasmine root extract comprises:

[0015] Raw material preparation

[0016] Collect fresh jasmine roots, clean them, and cut them into small pieces of 1-2 cm;

[0017] Micro-expansion treatment

[0018] Microwave power 800-1000 W, treatment at 60-70 ° C for 30 seconds to 1 minute;

[0019] Freeze drying

[0020] The pre-freezing temperature is -50℃, the time is 4 hours; the primary drying temperature is gradually increased from -35℃ to -20℃, the vacuum degree is 20Pa, and the time is 36 hours; the secondary drying temperature is gradually increased to 25℃, the vacuum degree is 20Pa, and the time is 18h;

[0021] Low temperature cold soak

[0022] Use 30-50% ethanol as solvent and soak at 4°C for 24-48 hours;

[0023] Ultrasound-assisted extraction

[0024] Ultrasonic frequency 20-40kHz, power density 0.5-1.0W / cm 2 , each treatment lasts 15 minutes, with an interval of 2 hours, for a total of 6-12 times;

[0025] Vacuum distillation and filtration

[0026] Vacuum distillation:

[0027] Use a rotary evaporator to perform vacuum distillation for 1-3 hours, the temperature does not exceed 40°C, and the pressure is 10-30mbar;

[0028] Filter method:

[0029] Primary filtration: using membrane filtration system, membrane pore size 0.45μm, pressure 0.1-0.3MPa, room temperature operation;

[0030] Fine filtration: Use microporous membrane filter with a pore size of 0.22μm, a pressure of 0.1-0.3MPa, and operate at room temperature.

[0031] Preferably, in the method for preparing the rice wine having sleep-aiding and anti-fatigue effects, the method for preparing the rosemary extract comprises:

[0032] Raw material preparation and drying

[0033] Choose fresh rosemary leaves, wash them clean, and dry them in a low-temperature dryer at a temperature not exceeding 40°C;

[0034] Crushing and sieving

[0035] Grind into fine powder and sieve to 60 mesh;

[0036] Low temperature cold soak

[0037] Use 50-70% ethanol as solvent and soak at 4°C for 24-48 hours;

[0038] Ultrasound-assisted extraction

[0039] Ultrasonic frequency 20-40kHz, power density 0.5-1.0W / cm 2 , each treatment lasts 15 minutes, with an interval of 2 hours, for a total of 6-12 times;

[0040] Vacuum distillation and filtration

[0041] Vacuum distillation:

[0042] Use a rotary evaporator to perform vacuum distillation for 1-3 hours, the temperature does not exceed 40°C, and the pressure is 10-30mbar;

[0043] Filter method:

[0044] Primary filtration: using membrane filtration system, membrane pore size 0.45μm, pressure 0.1-0.3MPa, room temperature operation;

[0045] Fine filtration: Use microporous membrane filter, the pore size of the microporous membrane is 0.22μm, the pressure is 0.1-0.3MPa, and the operation is at room temperature;

[0046] Antioxidant Addition

[0047] Natural antioxidants are added in an amount of 0.1-1% by weight of the rosemary extract.

[0048] Preferably, in the method for preparing the rice wine having sleep-aiding and anti-fatigue effects, the method for preparing the notoginseng and tangerine peel extracts comprises:

[0049] Raw material preparation and drying

[0050] Drying the rattan notoginseng and tangerine peel at a temperature not exceeding 40°C;

[0051] Grinding and mixing

[0052] Grind into fine powder, mix in a mass ratio of 1:3 to obtain mixed powder;

[0053] Ultrasonic extraction

[0054] The mixed powder was mixed with 5% rice wine at a mass ratio of 3-5:100, and then ultrasonic extraction was performed with an ultrasonic frequency of 20-40 kHz and a power density of 0.5-1.0 W / cm 2 , extraction time is 30-60min;

[0055] filter

[0056] Primary filtration: using membrane filtration system, membrane pore size 0.45μm, pressure 0.1-0.3MPa, room temperature operation;

[0057] Fine filtration: Use microporous membrane filter with a pore size of 0.22μm, a pressure of 0.1-0.3MPa, and operate at room temperature.

[0058] Preferably, in the method for preparing the rice wine with sleep-aiding and anti-fatigue effects, jasmine root extract, rosemary extract, notoginseng and tangerine peel extract are mixed in a mass ratio of 3-5:1-2:1-2 to obtain a mixed extract.

[0059] Preferably, in the method for preparing the rice wine with sleep-aiding and anti-fatigue effects, the mixed extract and the base wine are mixed in a mass ratio of 0.5-0.8:10-14.

[0060] Preferably, in the method for preparing the rice wine having sleep-aiding and anti-fatigue effects, food-grade carbon dioxide filling equipment is used to fill carbon dioxide, the pressure of the filled carbon dioxide is controlled at 2-4 bar, and the mixture is left to stand for 1 hour.

[0061] Preferably, in the method for preparing the rice wine with sleep-aiding and anti-fatigue effects, the natural antioxidant is vitamin C.

[0062] Preferably, in the method for preparing the rice wine with sleep-aiding and anti-fatigue effects, jasmine root extract, rosemary extract, notoginseng and tangerine peel extract are mixed in a mass ratio of 4.5:2:1.5.

[0063] Preferably, in the method for preparing the rice wine with sleep-aiding and anti-fatigue effects, the mixed extract and the base wine are mixed in a mass ratio of 0.8:13.

[0064] The present invention has at least the following beneficial effects:

[0065] The present application relates to a method for preparing rice wine with sleep-inducing and anti-fatigue effects, which makes full use of the synergistic effects of jasmine root extract, rosemary extract, notoginseng extract and tangerine peel extract through scientific proportioning and advanced preparation technology. The following are the main beneficial effects of the present application:

[0066] 1. Outstanding safety

[0067] No drug dependence: Unlike traditional drugs (such as diazepam), all ingredients in sample H are natural plant extracts, which will not cause drug dependence or addiction and are suitable for long-term use.

[0068] No serious side effects: Strict experimental verification showed that sample H had no obvious side effects, ensuring its safety and tolerability. This makes the product suitable not only for short-term relief of sleep problems and fatigue, but also for long-term maintenance of a good living condition.

[0069] Comply with food safety standards: Strictly control the sterilization steps to ensure that the product is contaminant-free during the production process, comply with food safety standards, and consumers can use it with confidence.

[0070] 2. Synergistic Effect of Multiple Ingredients

[0071] Jasmine Root Extract + Rosemary Extract + Notoginseng Extract + Tangerine Peel Extract

[0072] Multiple effects superposition:

[0073] Jasmine root extract: Contains a variety of volatile oils and alkaloids, such as indoles and terpenes, which have significant sedative and relaxing effects, helping to reduce anxiety and promote sleep.

[0074] Rosemary Extract: Rich in antioxidants such as carnosic acid, it can reduce stress and fatigue while providing neuroprotection and improving cognitive function.

[0075] Notoginseng Extract: Contains flavonoids and other bioactive ingredients, which can regulate the nervous system, help relieve tension and improve overall comfort.

[0076] Tangerine peel extract: As a traditional Chinese medicine, it can regulate qi and strengthen the spleen, aid digestion, indirectly relieve the discomfort caused by indigestion, and further enhance overall comfort and recovery ability.

[0077] Balance and complementarity:

[0078] Jasmine Root & Rosemary: Working together, these two not only enhance calming effects, but also provide powerful antioxidant support to reduce stress and fatigue.

[0079] Notoginseng and dried tangerine peel: The two complement each other, regulating the nervous system while improving gastrointestinal function, ensuring the harmony and stability of the body's internal and external environment.

[0080] Maximize the overall efficacy: The carefully designed ingredient ratio ensures the balance between the ingredients, avoiding the side effects that may be caused by excessive use of a single ingredient, while also maximizing the overall efficacy. The four ingredients work together to form a complete sleep-aiding and anti-fatigue system, significantly improving the overall effect of the product.

[0081] 3. Advanced extraction technology and its effects

[0082] Micro-expansion treatment

[0083] Improve extraction efficiency: Micro-expansion treatment uses short-term microwave treatment to quickly evaporate a small amount of water in the jasmine root, forming water molecule channels, causing its structure to become loose and porous. This structural change makes it easier for the soaking liquid to enter the jasmine root during the subsequent cold soaking extraction process, thereby significantly reducing the soaking time and improving the extraction efficiency.

[0084] Retain more active ingredients: This technology can retain the bioactive ingredients to the greatest extent possible without destroying the plant cell structure, ensuring the high purity and high efficacy of the final product.

[0085] Specific effect: After micro-expansion treatment, the extraction efficiency of jasmine roots increased by about 10%, and the release rate of active ingredients also increased significantly, which greatly improved the quality and effect of the final product.

[0086] Freeze drying

[0087] Maintain ingredient stability: Freeze-drying technology effectively avoids the damage of sensitive ingredients to high temperatures through low-temperature vacuum drying, maintaining the stability and integrity of active ingredients.

[0088] Extended Shelf Life: Freeze-dried extracts have extremely low moisture content, reducing the possibility of microbial growth, thereby extending the shelf life of the product and ensuring effectiveness during long-term storage.

[0089] Cold soak extraction

[0090] Mild extraction conditions: Cold soaking extraction uses a low-temperature soaking method to avoid the damage to sensitive components caused by high-temperature treatment and to retain the active substances in the plant to the maximum extent.

[0091] Enhanced flavor and aroma: Cold soaking extraction not only retains the active ingredients, but also better preserves the natural flavor and aroma of the plant, improving the taste and experience of the product.

[0092] 4. Market Potential

[0093] Market demand: With the accelerated pace of modern life, more and more people are facing sleep problems and fatigue, and this product has a broad market demand.

[0094] Summary of the beneficial effects

[0095] In summary, the rice wine of the present application not only performs well in the short term, but is also safer and more sustainable in long-term use. It has significant sleep-aiding and anti-fatigue effects, and the synergistic effects of jasmine root extract, rosemary extract, notoginseng extract and tangerine peel extract ensure the feasibility of efficient, safe and long-term use.

[0096] Therefore, the rice wine of the present application is a recommended functional beverage, suitable for people who need to relax and recover in modern fast-paced life, and can meet market demand and improve consumer satisfaction.

[0097] Other advantages, objectives and features of the present invention will be embodied in part through the following description, and in part will be understood by those skilled in the art through study and practice of the present invention. DETAILED DESCRIPTION

[0098] The present invention is further described in detail below in conjunction with embodiments so that those skilled in the art can implement the invention with reference to the description.

[0099] Experimental plan: Evaluation of the effect of rice wine in helping sleep and fighting fatigue

[0100] 1. Experimental Design and Implementation

[0101] 1. Overview of the Embodiments

[0102] In order to systematically evaluate the sleep-inducing and anti-fatigue effects of rice wine and determine the best formula, a detailed experiment was conducted. The following is the specific experimental design, implementation steps and experimental data.

[0103] 2. Sample preparation method (optimal parameters)

[0104] (1) Preparation of Jasmine Root Extract

[0105] Raw material preparation: Wash freshly collected or dried jasmine roots and cut them into 1 cm pieces.

[0106] Micro-puffing treatment: microwave power 900W, using an industrial microwave dryer, at 65°C for 45 seconds.

[0107] Freeze drying: pre-freezing temperature is -50℃, time is 4 hours; primary drying temperature gradually increases from -35℃ to -25℃, vacuum degree is 20Pa, time is 36 hours; secondary drying temperature gradually increases to 25℃, vacuum degree is 20Pa, time is 18 hours.

[0108] Low temperature cold soaking: Use 40% ethanol by volume as the solvent and soak at 4°C for 36 hours.

[0109] Ultrasonic assisted extraction: Use a dedicated ultrasonic extractor with a frequency of 30kHz and a power density of 0.75W / cm 2 Each treatment lasts 15 minutes, with an interval of 2 hours, and a total of 9 times.

[0110] Vacuum distillation and filtration:

[0111] Vacuum distillation: Use a rotary evaporator, control the temperature at 35°C, the pressure at 20 mbar, and the processing time for 2 hours.

[0112] Filtration: Use a membrane filtration system, select a filter membrane with a pore size of 0.45μm for primary filtration, a pressure of 0.2MPa, and operate at room temperature, and then use a microporous filter membrane with a pore size of 0.22μm for fine filtration, a pressure of 0.2MPa, and operate at room temperature.

[0113] (2) Preparation of Rosemary Extract

[0114] Raw material preparation and drying: Select fresh rosemary leaves, wash them clean, and dry them at 35°C for 4 hours.

[0115] Crush and sift: Crush the dried rosemary leaves into fine powder and sieve to 60 mesh.

[0116] Low temperature cold soaking and ultrasound-assisted extraction: 60% ethanol was used as the solvent, soaked at 4°C for 36 hours, and then ultrasound-assisted extraction was performed at a frequency of 30 kHz and a power density of 0.75 W / cm 2 Each treatment lasts 15 minutes, with an interval of 2 hours, and a total of 9 times.

[0117] Vacuum distillation and filtration:

[0118] Vacuum distillation: Use a rotary evaporator, control the temperature at 35°C, the pressure at 20 mbar, and the processing time for 2 hours.

[0119] Filtration: Use a membrane filtration system, select a filter membrane with a pore size of 0.45μm for primary filtration, a pressure of 0.2MPa, and operate at room temperature, and then use a microporous filter membrane with a pore size of 0.22μm for fine filtration, a pressure of 0.2MPa, and operate at room temperature.

[0120] Antioxidant addition: natural antioxidant (vitamin C) added in an amount of 0.5% of the mass of rosemary extract, and stored in a sealed container at 4°C in a refrigerator.

[0121] (3) Preparation of Radix Notoginseng and Citrus Reticulatae Extract

[0122] Raw material preparation and pretreatment

[0123] Cleaning:

[0124] Wash the freshly collected Notoginseng and dried tangerine peel separately with clean water to remove dirt, dust and other impurities on the surface.

[0125] For Panax notoginseng, you can brush it gently to avoid damaging the epidermis; for tangerine peel, you can wipe it gently with a soft cloth.

[0126] Initial drying:

[0127] The cleaned Panax notoginseng and tangerine peel are placed in a well-ventilated environment to dry to remove excess moisture and reduce the time and energy consumption of subsequent drying.

[0128] drying

[0129] Objective: To control the moisture content of Radix Notoginseng and Pericarpium Citri Reticulatae within an appropriate range to prevent mildew and retain the active ingredients to the maximum extent.

[0130] Rhizoma Panax Notoginseng:

[0131] Temperature: 40℃

[0132] Time: 4 hours (can be adjusted according to actual situation)

[0133] Target moisture content: 8-10%

[0134] Tangerine peel:

[0135] Temperature: 35℃

[0136] Time: 4 hours (can be adjusted according to actual situation)

[0137] Target moisture content: 8-10%

[0138] Equipment selection:

[0139] Using a low-temperature dryer can efficiently remove moisture at a lower temperature and reduce the loss of heat-sensitive components.

[0140] monitor:

[0141] Monitor moisture content regularly using a moisture meter or gravimetric method to ensure that target values ​​are being achieved.

[0142] Observe the material for changes in color, texture, and smell, and avoid over-drying that can cause discoloration or odor.

[0143] Grinding

[0144] Objective: To grind dried Notoginseng and dried orange peel into fine powder to increase the surface area and improve the extraction rate of effective ingredients.

[0145] Particle size requirement: Pass through a 100 mesh sieve (particle size is about 149 microns) to ensure that the powder is fine and uniform.

[0146] Equipment selection: Choose ball mill for grinding.

[0147] Grinding time: 5-15 minutes. The particle size of the powder needs to be checked regularly to ensure that it meets the required standards.

[0148] Temperature control: Pay attention to temperature control during grinding to avoid the loss of active ingredients due to frictional heat. Therefore, grind in a low temperature environment or use intermittent grinding to reduce heat accumulation.

[0149] mix

[0150] Purpose: To mix the ground Notoginseng and Tangerine Peel powder in proportion to ensure that the two ingredients are evenly distributed.

[0151] Mixing ratio: Mix notoginseng and tangerine peel powder in a mass ratio of 1:3.

[0152] Mixing Method: Use a low speed mixer to mix thoroughly to ensure both ingredients are evenly distributed.

[0153] Mixing time: The mixing time is generally 10-20 minutes, depending on the type of equipment and the amount of mixing. Sampling can be used to confirm the uniformity of the mix.

[0154] Ultrasonic assisted extraction

[0155] Objective: To improve the extraction efficiency and purity of active ingredients through ultrasonic-assisted extraction.

[0156] Solvent selection: Use rice wine with a volume fraction of 5% as the solvent.

[0157] Mixing ratio: The mixed powder of Radix Notoginseng and Pericarpium Citri Reticulatae is mixed with 5% rice wine at a mass ratio of 4:100.

[0158] Extraction conditions:

[0159] Frequency: 30kHz

[0160] Power density: 0.75W / cm 2

[0161] Extraction time: 45 minutes

[0162] Temperature: Room temperature (about 25°C)

[0163] Equipment selection: Use a dedicated ultrasonic extractor for extraction.

[0164] filter

[0165] Purpose: To remove solid residues from the extract and ensure the purity of the extract.

[0166] Filtering steps:

[0167] Primary filtration: Use a filter membrane with a pore size of 0.45μm for primary filtration, a pressure of 0.1-0.3MPa, and operate at room temperature to remove larger particles.

[0168] Fine filtration: Use a microporous filter membrane with a pore size of 0.22μm for fine filtration, with a pressure of 0.1-0.3MPa and room temperature to ensure the clarity of the extract.

[0169] store

[0170] Purpose: To ensure the stability and long-term storage of the extract.

[0171] Container selection: Use airtight containers to store the extract to avoid reabsorption of moisture and protect it from the effects of light and air oxidation.

[0172] Storage Conditions: Store in a cool, dry place at around 4°C to extend the shelf life.

[0173] (4) Mixing

[0174] The jasmine root extract, rosemary extract, notoginseng extract and tangerine peel extract are mixed in a certain proportion to obtain a mixed extract.

[0175] (4) Product preparation

[0176] The mixed extract is mixed evenly with the base wine, and then carbon dioxide is injected, filled and sterilized.

[0177] 2. Specific implementation methods of eight mixed sample preparations

[0178] When preparing the eight mixed samples, the amounts of jasmine root extract, rosemary extract, notoginseng and tangerine peel extract, and base wine are shown in Table 1.

[0179] Table 1 Amounts of extract and base wine used in the preparation of eight mixed samples

[0180]

[0181]

[0182] 3. Single comparative ratio and drug comparative ratio

[0183] Single comparison

[0184] Sample I: Contains only jasmine root extract, with the same formulation as sample A, but without the other two extracts.

[0185] Sample J: Contains only rosemary extract, with the same formulation as sample B but without the other two extracts.

[0186] Sample K: Contains only Radix Notoginseng and Citrus Reticulatae Extracts, with the same formula as sample C, but does not contain the other two extracts.

[0187] Drug comparison ratio

[0188] Sample L: Commercially available diazepam was used as a drug control sample and added to the base wine according to the recommended dosage to compare its sleep-inducing effect.

[0189] 4. Test methods

[0190] Subject selection

[0191] A total of 240 healthy volunteers were selected, ranging in age from 20-60 years old, with no known history of drug allergies or other health problems.

[0192] Randomization

[0193] The volunteers were randomly divided into 12 groups, 20 people in each group, and each group took a specific sample, including a placebo and a drug control ratio.

[0194] Taking cycle

[0195] Set a fixed taking cycle of 7 days and take it once every night at a fixed time before going to bed.

[0196] Data Collection

[0197] The volunteers' sleep quality and fatigue level were recorded before and after taking the drug, and data were collected using questionnaires, sleep monitors, etc.

[0198] The main evaluation indicators include:

[0199] Sleep latency (time to fall asleep)

[0200] Sleep duration

[0201] Sleep efficiency (percentage of time in bed spent actually asleep)

[0202] Daytime sleepiness

[0203] Fatigue score

[0204] 5. Experimental results and data

[0205] The experimental results are shown in Table 2.

[0206] Table 2 Experimental results

[0207]

[0208]

[0209] 6. Data Analysis and Conclusion

[0210] From the above specific experimental data, we can see that:

[0211] The mixed samples (samples AH) significantly improved sleep quality and reduced daytime sleepiness and fatigue, especially samples C, D, G and H, and sample H showed the best effect, which was close to the effect of diazepam in some indicators, and there was no problem of drug dependence.

[0212] The single-ingredient sample (sample IK) also showed certain sleep-aiding and anti-fatigue effects, but not as significant as the mixed sample.

[0213] The placebo group had the weakest effect, suggesting that the functional ingredients do have a positive effect on aiding sleep and fighting fatigue.

[0214] Although diazepam performs better in some indicators as a drug comparison, it has potential drug dependence and side effects and is not suitable for long-term use.

[0215] Taking into account the effect and safety, sample H (4.5 parts of jasmine root extract, 2 parts of rosemary extract, 1.5 parts of rattan notoginseng and tangerine peel extract) is one of the best formulas for rice wine with sleep-inducing and anti-fatigue effects. It performs well in all evaluation indicators and has no drug dependence problem, making it suitable for long-term use.

[0216] Therefore, sample H can be used as the best formula to be finally launched on the market, and after further optimization, it is expected to become a popular functional beverage.

[0217] VII. Safety test of sample H

[0218] In order to comprehensively evaluate the safety of sample H and ensure its reliability under different conditions and safety in long-term use, the following final safety experiment was conducted. The following is a detailed experimental design, implementation steps and specific experimental results data.

[0219] 1. Acute toxicity test (repeated verification)

[0220] Purpose: To verify the conclusion that sample H has no acute toxicity and to ensure the repeatability of the results.

[0221] Experimental design

[0222] Test animals: 60 healthy mice (ICR strain), weighing 20-25 grams.

[0223] Grouping:

[0224] Control group: given an equal amount of distilled water.

[0225] Experimental group: The maximum possible dose of sample H (5 g / kg body weight) was orally administered.

[0226] Positive control group: paraquat (20 mg / kg body weight) was orally administered.

[0227] Observation indicators

[0228] Behavioral changes (activity level, appetite, coat condition, etc.)

[0229] Weight changes

[0230] mortality rate

[0231] Experimental period: 7 days

[0232] The experimental results are shown in Table 3.

[0233] Table 3 Experimental results

[0234]

[0235] Conclusion: Sample H did not show any obvious toxic reactions or death in the acute toxicity experiment, proving that it has no acute toxicity and the results are repeatable.

[0236] 2. Long-term toxicity experiments

[0237] Objective: To evaluate the safety of long-term use of sample H.

[0238] Experimental design

[0239] Test animals: 60 healthy rats (Sprague-Dawley strain), weighing 200-250 grams.

[0240] Grouping:

[0241] Control group: given an equal amount of distilled water.

[0242] Experimental group: A conventional dose of sample H (0.5 g / kg body weight) was orally administered daily.

[0243] Positive control group: diazepam (1 mg / kg body weight) was administered daily.

[0244] Detection indicators

[0245] Blood biochemical indicators (liver function, kidney function)

[0246] Pathological biopsy (liver, kidney, heart)

[0247] Experimental period: 180 days

[0248] The experimental results are shown in Table 4.

[0249] Table 4 Experimental results

[0250]

[0251] Conclusion: Long-term use of sample H will not cause obvious organ damage or functional abnormality, proving its safety for long-term use.

[0252] 3. Dependence and addiction experiments

[0253] Objective: To evaluate whether sample H would cause dependence and addiction.

[0254] Experimental design

[0255] Test animals: 60 healthy mice (ICR strain), weighing 20-25 grams.

[0256] Grouping:

[0257] Control group: given an equal amount of distilled water.

[0258] Experimental group: A conventional dose of sample H (0.5 g / kg body weight) was orally administered daily.

[0259] Positive control group: diazepam (according to the clinically recommended dose) was administered daily.

[0260] Dosage cycle: Stop the drug after 30 days of continuous administration and observe withdrawal symptoms.

[0261] Observation indicators

[0262] Withdrawal symptoms (anxiety, irritability, loss of appetite, etc.)

[0263] The experimental results are shown in Table 5.

[0264] Table 5 Experimental results

[0265] Group Withdrawal symptom score (0-10) Main withdrawal symptoms Control group 0 No obvious withdrawal symptoms Experimental group (sample H) 0 No obvious withdrawal symptoms Positive control group 7±2 Obvious anxiety, irritability, and loss of appetite

[0266] Conclusion: Sample H will not cause dependence and addiction, and is superior to traditional drugs (such as diazepam).

[0267] 4. Allergic reaction test

[0268] Objective: To evaluate the tolerability and potential allergic reactions of sample H.

[0269] Experimental Design Subjects: 20 volunteers known to be sensitive to plant extracts were selected.

[0270] Administration: Oral administration, the dosage is the conventional dosage of sample H.

[0271] Observation time: 7 consecutive days, recording skin reactions, respiratory system symptoms, etc.

[0272] Observation indicators

[0273] Skin reactions (redness, swelling, itching, etc.)

[0274] Respiratory symptoms (coughing, wheezing, etc.)

[0275] The experimental results are shown in Table 6.

[0276] Table 6 Experimental results

[0277]

[0278] Conclusion: All subjects did not show obvious allergic reactions, which proved that sample H had good tolerance.

[0279] 5. Metabolite Analysis

[0280] Purpose: To evaluate the metabolism of sample H in vivo and ensure that no harmful metabolites accumulate.

[0281] Experimental design

[0282] Test animals: 30 healthy rats (Sprague-Dawley strain), weighing 200-250 grams.

[0283] Grouping:

[0284] Control group: given an equal amount of distilled water.

[0285] Experimental group: A conventional dose of sample H (0.5 g / kg body weight) was orally administered daily.

[0286] Experimental period: 90 days

[0287] Detection indicators

[0288] Metabolites in urine and blood were analyzed by liquid chromatography-mass spectrometry (LC-MS / MS).

[0289] Experimental Results

[0290] Major metabolites: The major metabolites detected included indole compounds from jasmine root extract, carnosic acid from rosemary extract, flavonoids from notoginseng extract, and citrus alkaloids from tangerine peel extract.

[0291] Deleterious metabolites: No deleterious metabolites or abnormal metabolic pathways were detected.

[0292] Conclusion: Sample H is metabolically stable in vivo, with no accumulation of harmful metabolites, further demonstrating its safety.

[0293] 8. Conclusion

[0294] Through the above series of final safety experiments, the safety of sample H can be fully evaluated:

[0295] Acute toxicity test (repeated verification): Sample H has no acute toxicity and the results are reproducible.

[0296] Long-term toxicity experiment: Long-term use of sample H did not cause obvious organ damage or functional abnormality, proving its safety for long-term use.

[0297] Dependence and addiction experiments: Sample H does not cause dependence and addiction, which is superior to traditional drugs.

[0298] Allergic reaction experiment: Sample H has good tolerance, and even people who are sensitive to plant extracts do not show obvious allergic reactions.

[0299] Metabolite analysis: Sample H is metabolically stable in vivo, with no accumulation of harmful metabolites.

[0300] This experimental protocol comprehensively and in detail describes the preparation method of rice wine, experimental design, implementation steps, data collection and analysis conclusions. The results show that sample H, as a sleep-aiding and anti-fatigue formula, exhibits significant efficacy, can effectively improve sleep quality, and significantly reduce daytime sleepiness and fatigue.

[0301] Through systematic experimental evaluation, sample H demonstrated superior performance in multiple key indicators, including shortening the time to fall asleep, prolonging sleep duration, improving sleep efficiency, and reducing daytime sleepiness and fatigue scores. Compared with placebo and other single-ingredient samples, sample H has a particularly outstanding effect, and has shown good tolerability and non-dependence in safety tests, making it suitable for long-term use.

[0302] Therefore, sample H was proved to be a very effective sleep-aiding and anti-fatigue formula, which has the effect of significantly improving sleep quality and reducing daytime sleepiness and fatigue, and provides a solid foundation for the development of functional beverages.

[0303] Although the embodiments of the present invention have been disclosed as above, they are not limited to the applications listed in the specification and the implementation modes. They can be fully applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the present invention is not limited to the specific details and the embodiments shown and described herein.

Claims

1. A method for preparing rice wine having sleep-aiding and anti-fatigue effects, characterized in that: include: The jasmine root extract, rosemary extract, notoginseng and tangerine peel extract are mixed to obtain a mixed extract; The mixed extract is mixed evenly with the base wine, and then carbon dioxide is injected, filled, and sterilized to obtain rice wine.

2. The method for preparing the rice wine having sleep-aiding and anti-fatigue effects as claimed in claim 1, characterized in that: The preparation method of jasmine root extract comprises: Raw material preparation Collect fresh jasmine roots, clean them, and cut them into small pieces of 1-2 cm; Micro-expansion treatment Microwave power 800-1000 W, treatment at 60-70 ° C for 30 seconds to 1 minute; Freeze drying The pre-freezing temperature is -50℃, the time is 4 hours; the primary drying temperature is gradually increased from -35℃ to -20℃, the vacuum degree is 20Pa, and the time is 36 hours; the secondary drying temperature is gradually increased to 25℃, the vacuum degree is 20Pa, and the time is 18h; Low temperature cold soak Use 30-50% ethanol as solvent and soak at 4°C for 24-48 hours; Ultrasound-assisted extraction Ultrasonic frequency 20-40kHz, power density 0.5-1.0W / cm 2 , each treatment lasts 15 minutes, with an interval of 2 hours, for a total of 6-12 times; Vacuum distillation and filtration Vacuum distillation: Use a rotary evaporator to perform vacuum distillation for 1-3 hours, the temperature does not exceed 40°C, and the pressure is 10-30mbar; Filter method: Primary filtration: using membrane filtration system, membrane pore size 0.45μm, pressure 0.1-0.3MPa, room temperature operation; Fine filtration: Use microporous membrane filter with a pore size of 0.22μm, a pressure of 0.1-0.3MPa, and operate at room temperature.

3. The method for preparing the rice wine having sleep-aiding and anti-fatigue effects as claimed in claim 2, characterized in that: The preparation method of the rosemary extract comprises: Raw material preparation and drying Choose fresh rosemary leaves, wash them clean, and dry them in a low-temperature dryer at a temperature not exceeding 40°C; Crushing and sieving Grind into fine powder and sieve to 60 mesh; Low temperature cold soak Use 50-70% ethanol as solvent and soak at 4°C for 24-48 hours; Ultrasound-assisted extraction Ultrasonic frequency 20-40kHz, power density 0.5-1.0W / cm 2 , each treatment lasts 15 minutes, with an interval of 2 hours, for a total of 6-12 times; Vacuum distillation and filtration Vacuum distillation: Use a rotary evaporator to perform vacuum distillation for 1-3 hours, the temperature does not exceed 40°C, and the pressure is 10-30mbar; Filter method: Primary filtration: using membrane filtration system, membrane pore size 0.45μm, pressure 0.1-0.3MPa, room temperature operation; Fine filtration: Use microporous membrane filter, the pore size of the microporous membrane is 0.22μm, the pressure is 0.1-0.3MPa, and the operation is at room temperature; Antioxidant Addition Natural antioxidants are added in an amount of 0.1-1% by weight of the rosemary extract.

4. The method for preparing the rice wine having sleep-aiding and anti-fatigue effects as claimed in claim 3, characterized in that: The preparation method of the notoginseng and tangerine peel extract comprises: Raw material preparation and drying Drying the rattan notoginseng and tangerine peel at a temperature not exceeding 40°C; Grinding and mixing Grind into fine powder, mix in a mass ratio of 1:3 to obtain mixed powder; Ultrasonic extraction The mixed powder was mixed with 5% rice wine at a mass ratio of 3-5:100, and then ultrasonic extraction was performed with an ultrasonic frequency of 20-40 kHz and a power density of 0.5-1.0 W / cm 2 , extraction time is 30-60min; filter Primary filtration: using membrane filtration system, membrane pore size 0.45μm, pressure 0.1-0.3MPa, room temperature operation; Fine filtration: Use microporous membrane filter with a pore size of 0.22μm, a pressure of 0.1-0.3MPa, and operate at room temperature.

5. The method for preparing the rice wine having sleep-aiding and anti-fatigue effects as claimed in claim 4, characterized in that: The jasmine root extract, rosemary extract, notoginseng and tangerine peel extract are mixed in a mass ratio of 3-5:1-2:1-2 to obtain a mixed extract.

6. The method for preparing the rice wine having sleep-aiding and anti-fatigue effects as claimed in claim 5, characterized in that: The mixed extract and the base wine are mixed in a mass ratio of 0.5-0.8:10-14.

7. The method for preparing rice wine having sleep-aiding and anti-fatigue effects as claimed in claim 1, characterized in that: Use food-grade carbon dioxide filling equipment to fill carbon dioxide, control the pressure of the filled carbon dioxide at 2-4bar, and let it stand for 1 hour.

8. The method for preparing the rice wine having sleep-aiding and anti-fatigue effects as claimed in claim 3, characterized in that: The natural antioxidant is vitamin C.

9. The method for preparing rice wine having sleep-aiding and anti-fatigue effects as claimed in claim 5, characterized in that: The jasmine root extract, rosemary extract, notoginseng and tangerine peel extract were mixed in a mass ratio of 4.5:2:1.

5.

10. The method for preparing rice wine having sleep-aiding and anti-fatigue effects as claimed in claim 6, characterized in that: The mixed extract and the base wine are mixed in a mass ratio of 0.8:13.

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