Amplification analyzer

Abstract

The amplification analyzer comprises a shell, a processor, a dispatching mechanism, an amplification mechanism, a signal analysis mechanism and a forward transfer mechanism, the shell comprises a mounting cavity and a forward transfer window, and a forward transfer position is arranged in the mounting cavity; the forward transfer mechanism is used for conveying consumables to be amplified to a forward transfer position through a forward transfer window; according to the technical scheme, the amplification analyzer can be connected with the pre-cascade equipment in a mode that the forward transfer mechanism extends out of the forward transfer window, so that consumables in the pre-cascade equipment are transferred to the forward transfer position of the mounting cavity through the transfer window. Afterwards, the processor of the amplification analyzer can control the scheduling mechanism to extract the consumables to be amplified from the forward transfer position to reach the amplification mechanism for amplification, after amplification is finished, the processor transfers the amplified consumables to the signal analysis mechanism for signal analysis, and cascade connection with other devices is achieved. And the use scene of the amplification analyzer is expanded.

Description

Technical Field

[0001] The present application relates to the technical field of PCR detection, and in particular to an amplification analyzer. Background Art

[0002] Digital polymerase chain reaction (dPCR) is a new method for nucleic acid detection and quantitative analysis. Its core principle is to divide the consumables to be tested into numerous tiny reaction partitions, perform independent PCR amplification in each partition, and finally calculate the concentration of the target by counting the number of positive and negative partitions, thereby realizing nucleic acid detection and analysis.

[0003] Amplification analyzers are an important part of digital PCR testing. Their main function is to amplify and analyze the segmented tiny reaction partitions, thereby achieving high sensitivity and accurate quantitative detection of nucleic acids. However, with the increase in detection scenarios, traditional amplification analyzers have long been unable to meet the growing detection needs. Summary of the invention

[0004] The purpose of this application is to provide an amplification analyzer, aiming to solve the problem in the related art that the amplification analyzer has poor scalability and is difficult to meet the detection needs of multiple scenarios.

[0005] In a first aspect, the present application provides an amplification analyzer, the amplification analyzer comprising a housing, a processor, a scheduling mechanism, an amplification mechanism, a signal analysis mechanism and a forward transport mechanism, the housing comprising an installation cavity and a forward transport window, the forward transport window being connected to the installation cavity, the scheduling mechanism, the amplification mechanism and the signal analysis mechanism being arranged in the installation cavity and being controlled by the processor; a forward transport position is arranged in the installation cavity;

[0006] The forward transport mechanism is used to transport the consumables to be amplified to the forward transport position through the forward transport window;

[0007] The processor is configured to control the scheduling mechanism to extract the consumables to be amplified from the forward transport position, transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism for signal analysis to determine and output corresponding analysis results.

[0008] In a possible implementation, the amplification analyzer is used to connect to a pre-cascade device;

[0009] One end of the forward transfer mechanism is disposed or extended inside the front cascade device, and the other end of the forward transfer mechanism is disposed or extended inside the installation cavity.

[0010] In a possible implementation, the amplification analyzer is also used to connect to a subsequent amplification analyzer; the housing also includes a rearward transport window for allowing a transport mechanism in the subsequent amplification analyzer to pass through and extend into the installation cavity.

[0011] In a possible implementation, the amplification analyzer further includes a consumables cache;

[0012] The processor is configured to control the scheduling mechanism to extract the consumables to be amplified from the forward transfer position to the consumable cache position for caching when the amplification mechanism is in a non-idle state; and to control the scheduling mechanism to extract the consumables to be amplified to the amplification mechanism for amplification when the amplification mechanism is in an idle state.

[0013] In a possible implementation, the forward transport mechanism extends in the installation cavity along a first direction, and the extension portion is at least partially located between the consumables buffer position and the amplification mechanism;

[0014] The consumable material buffer position and the amplification mechanism are arranged side by side along a second direction, wherein the first direction and the second direction are perpendicular to each other.

[0015] In a possible implementation, at least a portion of the forward transport mechanism extends in the installation cavity along a first direction, and the amplification mechanism and the signal analysis mechanism are arranged side by side along the first direction.

[0016] In a possible implementation, the amplification analyzer further includes a liquid path mechanism, which is used to provide an injection channel and a sample oil carrier channel; the liquid path mechanism and the signal analysis mechanism are arranged side by side along a second direction, wherein the first direction and the second direction are perpendicular to each other.

[0017] In a possible implementation, a first loading space extending along a first direction is provided between the signal analysis mechanism and the liquid path mechanism; the first loading space is used for at least partial installation of a transport mechanism in a post-amplification analyzer.

[0018] In a possible implementation, the forward transport mechanism includes:

[0019] A guide rail, the guide rail is arranged in the installation cavity and extends out of the installation cavity through the forward transfer window;

[0020] A loading seat, the loading seat being slidably disposed on the guide rail;

[0021] A rotating seat, the rotating seat is rotatably disposed on the loading seat, and has a first position extending along the first direction and a second position extending along the second direction relative to the loading seat, the first direction and the second direction are two perpendicular directions, and the consumables to be amplified are placed on the rotating seat; and

[0022] A reversing assembly is used to drive the rotating seat to rotate between the first position and the second position.

[0023] In a possible implementation, the reversing assembly includes a push member and an elastic member, wherein the push member is disposed on the movement path of the loading seat; the push member is configured to contact the rotating seat to push the rotating seat to rotate from the second position to the first position;

[0024] The elastic member is disposed between the rotating seat and the loading seat, and the elastic member is configured to drive the rotating seat to return from the first position to the second position when the push member is separated from the rotating seat.

[0025] In the embodiment of the present application, the amplification analyzer can be connected to the front cascade device by extending the forward transfer mechanism out of the forward transfer window, so that the consumables in the front cascade device can be transferred to the forward transfer position of the installation cavity through the forward transfer window. Afterwards, the processor of the amplification analyzer can control the scheduling mechanism to extract the consumables to be amplified at the forward transfer position to the amplification mechanism for amplification. After the amplification is completed, the processor transfers the consumables that have completed amplification to the signal analysis mechanism for signal analysis, thereby cascading with other devices to expand the use scenario of the amplification analyzer.

[0026] In a second aspect, the present application further proposes an amplification analyzer, the amplification analyzer comprising a housing, a processor, a scheduling mechanism, an amplification mechanism and a signal analysis mechanism, the housing comprising an installation cavity and a rearward transport window, the rearward transport window being in communication with the installation cavity, the installation cavity comprising a consumable loading position for loading consumables to be amplified, the scheduling mechanism, the amplification mechanism and the signal analysis mechanism being arranged in the installation cavity and all being controlled by the processor;

[0027] The backward transfer window is used for allowing the backward transfer mechanism to pass through;

[0028] The processor is configured to control the scheduling mechanism to extract the consumables to be amplified from the consumable loading position, transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism for signal analysis to determine and output the corresponding analysis results; and the processor also controls the scheduling mechanism to extract the consumables to be amplified from the consumable loading position, transfer them to the backward transfer position of the backward transfer mechanism, so that the backward transfer mechanism drives the consumables to be amplified through the backward transfer window and transports them to the post-amplification analyzer.

[0029] In one possible implementation, the processor is configured to, when the amplification mechanism is in a non-idle state, control the scheduling mechanism to extract the consumables to be amplified from the consumable loading position and transfer them to the backward transfer position of the backward transfer mechanism, either according to preset scheduling conditions or according to user instructions.

[0030] In a possible implementation, the housing further includes a loading window, and the consumable to be amplified is placed into the consumable loading position through the loading window;

[0031] The backward transfer window and the loading window are arranged on adjacent sides of the shell.

[0032] In a possible implementation, the amplification mechanism and the signal analysis mechanism are arranged side by side along a first direction, the consumable loading position and the amplification mechanism are arranged side by side along a second direction, and a second loading space extending along the first direction is provided in the installation cavity, and the second loading space is used for the installation of the backward transfer mechanism, wherein the first direction and the second direction are perpendicular to each other.

[0033] In one possible implementation, the amplification analyzer further includes a liquid path mechanism, which is used to provide an injection channel and a sample oil carrier channel; the liquid path mechanism and the signal analysis mechanism are arranged side by side along the second direction, and / or the second loading space is located between the liquid path mechanism and the signal analysis mechanism.

[0034] In the technical solution of the present application, the amplification analyzer has a backward transfer window for the backward transfer mechanism to extend into, and the amplification analyzer can be connected to the rear amplification analyzer through the backward transfer mechanism. On the one hand, the processor of the amplification analyzer can control the scheduling mechanism to extract the consumables to be amplified from the consumable loading position, and transfer them to the amplification mechanism for amplification. After the amplification is completed, the processor transfers the amplified consumables to the signal analysis mechanism for signal analysis, thereby completing the amplification analysis of part of the consumables; on the other hand, the processor of the amplification analyzer can also control the scheduling mechanism to extract the consumables to be amplified from the consumable loading position, and transfer them to the backward transfer position of the backward transfer mechanism, so that the backward transfer mechanism drives the consumables to be amplified through the backward transfer window and transports them to the rear amplification analyzer, so as to perform amplification analysis in the rear amplification analyzer, realize cascading with other devices, and expand the use scenario of the amplification analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the technical solutions of the implementation methods of the present application, the drawings required for the implementation methods will be briefly introduced below. Obviously, the drawings described below are only some implementation methods of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0036] Figure 1 A schematic diagram of the three-dimensional structure of an embodiment of the amplification analyzer provided in the present application;

[0037] Figure 2 for Figure 1 Schematic diagram of the three-dimensional structure when the amplification analyzer and the front cascade device are spliced;

[0038] Figure 3 for Figure 2 Schematic diagram of the internal structure;

[0039] Figure 4 for Figure 3 A schematic diagram of the structure of an embodiment of a scheduling mechanism grabbing a PCR tube plate;

[0040] Figure 5 for Figure 1 Schematic diagram of the operating environment of the amplification analyzer;

[0041] Figure 6 for Figure 1 Schematic diagram of the structure of the connection between the amplification analyzer and the front cascade device;

[0042] Figure 7 for Figure 1 Schematic diagram of the structure of the splicing of the mid-amplification analyzer and the post-amplification analyzer;

[0043] Figure 8 for Figure 1 A schematic diagram of the structure in which the middle amplification analyzer is simultaneously connected with the front cascade device and the rear amplification analyzer;

[0044] Fig. 9 for Figure 1 A schematic diagram of the structure of the amplification analyzer when operating alone;

[0045] Fig.10 for Figure 1 Schematic diagram of the structure of the intermediate transfer mechanism;

[0046] Fig.11 for Fig.10 A schematic structural diagram of an embodiment of a mobile platform;

[0047] Fig.12 for Fig.10 A cross-sectional view of

[0048] Fig.13 for Fig.10 Schematic diagram of the structure of the middle rotating seat at the beginning of reversing;

[0049] Fig.14 for Fig.10 Schematic diagram of the structure of the middle rotating seat during the reversing process;

[0050] Fig.15 for Fig.10 Schematic diagram of the structure after the middle rotating seat has been commutated;

[0051] Fig.16 A schematic diagram of the control flow of the first embodiment of the amplification analyzer provided in the embodiment of the present application;

[0052] Fig.17 The present invention is a flowchart of a processor determining a first placement position and a second placement position according to an operation mode.

[0053] Fig.18 This is a schematic diagram of the control flow of the amplification analyzer provided in an embodiment of the present application in the third online mode.

[0054] Description of reference numerals:

[0055] 1000-amplification analyzer;

[0056] 1-housing, 11-installation cavity, 111a-consumable material loading position, 111b-waste material placement position, 111c-consumable material buffer position, 12-transfer window, 12a-forward transfer window, 12b-rearward transfer window, 13-loading window;

[0057] 2-dispatching mechanism, 2a-mechanical gripper;

[0058] 3- Amplification mechanism;

[0059] 4-transfer mechanism, 41a-forward transfer position, 41b-backward transfer position, 4a-forward transfer mechanism, 4b-backward transfer mechanism, 42-guide rail, 43-moving platform, 44-driving assembly, 431-loading seat, 432-rotating seat, 433-reversing assembly, 4331-pushing member, 4332-elastic member, 4333-limiting member;

[0060] 5-Signal analysis agency;

[0061] 6-Liquid circuit mechanism;

[0062] 2000- cascade device, 2000a- pre-cascade device, 2000b- post-amplification analyzer;

[0063] 3000-consumables, 3100-PCR tubes and plates, 3200-gaps;

[0064] 1001 - processor, 1002 - communication bus, 1003 - user interface, 1004 - network interface, 1005 - memory. DETAILED DESCRIPTION

[0065] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0066] It should be noted that when a component is said to be "fixed to" another component, it can be directly on the other component or there can be a central component. When a component is said to be "connected to" another component, it can be directly connected to the other component or there can be a central component at the same time.

[0067] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used in this application and in the specification are only for the purpose of describing specific embodiments and are not intended to limit this application. The term "and / or" used in this application includes any and all combinations of one or more of the related listed items.

[0068] In conjunction with the accompanying drawings, some embodiments of the present application are described in detail below. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0069] For ease of understanding, the technical terms involved in this application are explained and described below.

[0070] The droplet preparation involved in this article refers to the generation of droplets of nucleic acid solution, that is, the generation of droplets through the microchannels of the chip well plate, so that the nucleic acid solution changes from a water state to an emulsified state (which can be an oil-in-water form, or a water-in-oil form). In some embodiments, droplet preparation can also be to collect droplets that have completed the reaction, and directly store the emulsified droplets in the PCR tube well plate, and this application does not limit this.

[0071] The system construction instrument involved in this article is used to perform sample pre-processing steps. In some embodiments, three processes of nucleic acid extraction, detection reagent preparation and detection reagent addition may be included. For example, when the nucleic acid to be detected is sent to the system construction instrument, the system construction instrument will extract the nucleic acid to be detected and encapsulate it in a first container. Subsequently, the system construction instrument will configure different detection reagents according to the different types of nucleic acids to be detected, and encapsulate the configured detection reagents in a second container. Finally, the system construction instrument will extract part of the nucleic acid solution in the first container, extract part of the detection reagent in the second container, mix it into a third container, and encapsulate it. Of course, nucleic acid extraction can also be performed in an independent nucleic acid extraction device, or it can be done manually, so the system construction instrument can only perform the configuration of the detection solution and the mixed packaging of the detection solution and nucleic acid, and this application does not limit this.

[0072] It should be noted that the consumables involved in this article have different definitions and functions at different stages. For example, before the sample pre-processing is performed, the consumables may refer to the original nucleic acid sample to be detected, and the sample includes DNA or RNA in the cell or tissue sample extracted from the organism. After the pre-sample processing is completed, the consumables may refer to the nucleic acid solution that has been extracted and mixed with the detection reagent. Before the droplet preparation is performed, the consumables may refer to the chip well plate and the PCR tube well plate storing the nucleic acid solution, wherein the chip in the chip well plate is used to promote the generation of droplets, and the PCR tube well plate is used to collect the droplets generated by the chip well plate. After the droplet preparation is completed and before the amplification analysis is performed, if the chip well plate and the PCR tube well plate are separately set, the chip well plate is discarded or recycled, and the consumables may refer to the PCR tube well plate storing nucleic acid droplets. If the chip well plate and the PCR tube well plate are integrally formed, the consumables may refer to an integrated chip well plate and a PCR tube well plate, and nucleic acid droplets are stored in the PCR tube well plate. After the amplification analysis is completed and before the signal analysis is performed, the consumables may refer to the PCR tube plate storing the nucleic acid solution after the polymerase chain reaction or the integrated chip plate and PCR tube plate. After the signal analysis is completed, the consumables may refer to the PCR tube plate storing the nucleic acid solution after the fluorescence reaction or the integrated chip plate and PCR tube plate. For ease of explanation, the materials in the above states are now referred to as consumables.

[0073] The user referred to in this article can be the person who purchases and uses the product, the product manufacturer, or the technical personnel in product research and development. This application does not impose any restrictions on this.

[0074] In conjunction with the accompanying drawings, some embodiments of the present application are described in detail below. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0075] Please refer to Figures 1 to 3 The present application proposes an amplification analyzer 1000, which is used to be spliced ​​with a cascade device 2000 to meet the needs of various detection scenarios. The amplification analyzer 1000 includes a housing 1, an amplification mechanism 3, a signal analysis mechanism 5, a processor, a memory, and a scheduling mechanism 2. The housing 1 serves as a supporting skeleton of the amplification analyzer 1000 and is used to support and connect the various parts of the amplification analyzer 1000.

[0076] The shell 1 is formed with an installation cavity 11, and the amplification mechanism 3, the signal analysis mechanism 5, the processor, the memory and the scheduling mechanism 2 are installed in the installation cavity 11. The shell 1 can be an open frame formed by overlapping only the structural skeleton, or it can be a sealed structure formed by the structural skeleton and the skin, and the present application does not limit this. In one embodiment of the present application, the installation cavity 11 is a closed chamber, and the air pressure in the installation cavity 11 is lower than the air pressure outside the installation cavity 11; in this way, it is ensured that the air in the installation cavity 11 can only flow from the installation cavity 11 to the outside of the installation cavity 11, thereby reducing the possibility of external contaminants of the amplification analyzer 1000 entering the installation cavity 11 and contaminating the consumables 3000.

[0077] In order to further reduce the possibility of contamination of the consumables 3000 to be amplified in the installation cavity 11, in another possible implementation mode of the present application, a high-efficiency air particulate filter (not shown in the figure) is also installed in the installation cavity 11, and the installation cavity 11 and the atmosphere outside the equipment exchange gases through the high-efficiency air particulate filter, thereby reducing the amount of pollutants entering the installation cavity 11 from the external atmosphere and reducing the risk of cross-contamination.

[0078] The dispatching mechanism 2 may be a mechanical gripper 2a, a conveyor belt, or a vacuum suction cup, and this application does not limit this. Figure 4 The dispatching mechanism 2 uses a mechanical gripper 2a. Correspondingly, a notch 3200 is provided on the side of the PCR tube plate 3100 to facilitate the dispatching mechanism 2 to grab it.

[0079] The amplification mechanism 3 is used to perform polymerase chain reaction amplification on the consumable 3000. Specifically, when the consumable 3000 is placed in the amplification mechanism 3, the heating module in the amplification mechanism 3 will heat the consumable 3000 and keep the temperature of the consumable 3000 within a preset denaturation temperature range, such as between 94°C and 98°C, the purpose of which is to separate the double-stranded DNA in the consumable 3000 into a single strand, and this process is called denaturation of the consumable 3000. Afterwards, the cooling module of the amplification mechanism 3 will quickly cool down the temperature of the consumable 3000 and keep the temperature of the consumable 3000 within a preset annealing temperature range, such as between 40°C and 65°C, the purpose of which is to allow the primer of the DNA polymerase to bind to the specific sequence of the target DNA, and this process is called annealing. Then, the amplification mechanism 3 will turn on the heating module again and heat the consumable 3000 to a preset extension temperature, such as 72°C, so that the nucleic acid in the consumable 3000 can synthesize a new DNA chain under the action of the polymerase and the primer. This process is called the extension of the consumable 3000. Finally, the amplification mechanism 3 repeats the above steps in the order of denaturation-annealing-extension until the nucleic acid in the consumable 3000 is fully reacted, thereby completing the amplification of the consumable 3000.

[0080] There may be one or more amplification mechanisms 3, and the present application does not impose any limitation on this. For example, in one embodiment of the present application, three amplification mechanisms 3 are arranged in the installation cavity 11 of the amplification analyzer 1000, and the three amplification mechanisms 3 are arranged at intervals and operate independently, so as to improve the amplification efficiency of the amplification analyzer 1000 for the consumables 3000.

[0081] The signal analysis mechanism 5 is used to detect the consumables after the amplification is completed. Specifically, when the consumables 3000 are transported from the amplification mechanism 3 to the signal analysis mechanism 5 under the transportation of the scheduling mechanism 2, the signal analysis mechanism 5 will use specific fluorescent dyes (such as SYBR Green or TaqMan probes) and other methods to monitor the fluorescent signal in the consumables 3000 in real time and record the changes in signal intensity. Afterwards, the signal analysis mechanism 5 will process and analyze the monitored fluorescent signal, collect the fluorescence data of each cycle and perform background correction, generate an amplification curve, and calculate the Ct value (threshold cycle number) of the target DNA according to the set threshold. By comparing with the standard curve, the signal analysis mechanism 5 can quantitatively analyze the initial concentration of the target DNA in the sample and ultimately generate the detection result of the nucleic acid.

[0082] Please refer to Figure 5 The processor 1001 is used to call computer instructions to control the operation of the scheduling mechanism 2 and the amplification mechanism 3. The memory is connected to the processor 1001, and the memory is used to store computer instructions for the processor 1001 to call.

[0083] In some embodiments, the amplification analyzer 1000 also includes a communication bus 1002, a user interface 1003, and a network interface 1004, and the communication bus 1002 is used to realize the connection communication between these components. The user interface 1003 is mainly used for data interaction by the user, and the user interface 1003 may include a display screen (Disp l ay), an input unit such as a keyboard (Keyboard), and the optional user interface 1003 may also include a standard wired interface and a wireless interface. The network interface 1004 is mainly used for data communication with a network server, and the network interface 1004 may optionally include a standard wired interface and a wireless interface (such as a wireless fidelity (Wi re less-Fidelity, Wi-Fi) interface).

[0084] Please refer to Figure 3 In some implementations, the housing 1 is provided with a transfer window 12 communicating with the installation cavity 11, a transfer mechanism 4 is provided in the installation cavity 11, and the memory stores at least two working modes. The processor 1001 can determine the working mode corresponding to the instruction according to the user's instruction, determine the first placement position of the consumables to be amplified according to the corresponding working mode and the specific working mode, and / or determine the second placement position of the consumables that have completed amplification, and also control the scheduling mechanism 2 to extract the consumables to be amplified in the first placement position, transfer it to the amplification mechanism 3 for amplification, and then transfer it to the signal analysis mechanism 5 for signal analysis to determine and output the corresponding analysis results, and finally transfer the consumables that have completed amplification to the second placement position.

[0085] The amplification analyzer of the embodiment of the present application can select different working modes according to different scenario requirements, and determine the corresponding first placement position and second placement position according to different working modes. After the scheduling mechanism extracts the consumables to be amplified in the first placement position and transfers them to the amplification mechanism for amplification, it is transferred to the signal analysis mechanism for signal analysis to determine and output the corresponding analysis results, and finally the consumables that have completed amplification are transferred to the second placement position, thereby completing the amplification and analysis of the consumables. By selecting different first placement positions and second placement positions, the amplification analyzer can not only be used as an independent device to amplify and analyze consumables, but can also be cascaded with other devices with the cooperation of the transfer window and the transfer mechanism, so as to expand the use scenarios of the amplification analyzer and improve the flexibility of the use of the amplification analyzer.

[0086] In some embodiments, there are many ways to set the transfer mechanism 4 and the transfer window 12. Figure 6The transfer window 12 is a forward transfer window 12a, and the transfer mechanism 4 is a forward transfer mechanism 4a. The amplification analyzer 1000 can be connected to the front cascade device 2000a through the forward transfer window 12a. One end of the forward transfer mechanism 4a is set or extended in the installation cavity 11, and the other end passes through the forward transfer window 12a and is set or extended in the front cascade device 2000a. The forward transfer mechanism 4a is used to transfer the consumables 3000 in the front cascade device 2000a to the amplification analyzer 1000.

[0087] Accordingly, a forward transfer position 41a for receiving consumables to be amplified is provided in the installation chamber 11. The forward transfer position 41a and the forward transfer mechanism 4a overlap at least part of the space in the installation chamber 11, and the forward transfer mechanism 4a transfers the consumables 3000 in the front cascade device 2000a to the forward transfer position 41a. In some embodiments, a waste placement position 111b for discarding consumables is provided in the installation chamber 11.

[0088] In some embodiments, the processor 1001 of the amplification analyzer 1000 calls the first online mode stored in the memory 1005. In the first online mode, the processor 1001 sets the forward transfer position 41a to the first placement position and the waste placement position 111b to the second placement position; after the consumable 3000 enters the installation cavity 11, the processor controls the scheduling mechanism 2 to extract the consumable 3000 to be amplified in the forward transfer position 41a, and transfers it to the amplification mechanism 3 for amplification, and then transfers it to the signal analysis mechanism 5 to determine and output the corresponding analysis results, and finally transfers the consumable 3000 that has completed the amplification to the waste placement position 111b, thereby completing the amplification analysis of the consumable 3000.

[0089] It should be noted that the forward transfer mechanism 4a can be pre-set in the amplification analyzer 1000; it can also be pre-set in the pre-cascade device 2000a; it can also be an independent component independent of the amplification analyzer 1000 and the pre-cascade device 2000a, and it is assembled and installed only when the amplification analyzer 1000 and the pre-cascade device 2000a are spliced, and the present application does not impose any restrictions on this.

[0090] In some embodiments, the forward transfer mechanism 4a is controlled by a processor of the amplification analyzer 1000. Specifically, the processor controls the operation of the forward transfer mechanism 4a and obtains the state of the forward transfer mechanism 4a. For example, under the control of the processor of the amplification analyzer 1000, the forward transfer mechanism 4a first transfers the consumables 3000 in the front cascade device 2000a to the forward transfer position 41a in the installation cavity 11 through the forward transfer window 12a, and then controls the scheduling mechanism 2 to extract the consumables 3000 in the forward transfer position 41a. In some embodiments, the forward transfer mechanism 4a is controlled by a processor of the front cascade device 2000a. Specifically, the processor controls the operation of the forward transfer mechanism 4a and obtains the state of the forward transfer mechanism 4a. For example, under the control of the processor of the pre-cascade device 2000a, the forward transport mechanism 4a first transports the consumables 3000 in the pre-cascade device 2000a to the forward transport position 41a of the installation chamber 11 through the forward transport window 12a, and then sends the state information of the forward transport mechanism 4a to the processor of the amplification analyzer 1000 through the direct or indirect (through the host computers of both) information interaction mechanism between the processor of the amplification analyzer 1000 and the processor of the pre-cascade device 2000a, so that the processor of the amplification analyzer 1000 controls the scheduling mechanism 2 to extract the consumables 3000 from the forward transport position 41a after determining that the consumables 3000 have been transported to the forward transport position 41a. In some embodiments, the forward transport mechanism 4a is controlled by the processors of the host computers of the amplification analyzer 1000 and the pre-cascade device 2000a. Specifically, the processor of the host computer directly or indirectly controls the operation of the forward transfer mechanism 4a, obtains the state of the forward transfer mechanism 4a, and based on the state, directly or indirectly controls the processor of the amplification analyzer 1000 to retrieve the consumables 3000 on the forward transfer position 41a.

[0091] In certain embodiments, the pre-cascade device 2000a is a droplet preparation instrument. The droplet preparation instrument can prepare droplets and / or collect droplets to the consumables 3000. That is, in the droplet preparation instrument, the droplet preparation mechanism of the droplet preparation instrument will place the nucleic acid solution to be detected in the droplet chip, and drive the nucleic acid solution to be detected to flow in the microchannel of the droplet chip through the pressure assembly, and form droplets. After the droplets are formed, the droplet preparation mechanism will collect the droplets. In certain embodiments, the consumables 3000 that have completed the collection of droplets will be transported to the forward transport position 41a of the installation chamber 11 through the forward transport window 12a under the transport of the forward transport mechanism 4a, so that the amplification analyzer 1000 completes subsequent amplification and analysis.

[0092] In some embodiments, the pre-cascade device 2000a is a pre-amplification analyzer. The splicing of the pre-amplification analyzer and the amplification analyzer 1000 can improve the throughput of the amplification analysis and improve the efficiency of the amplification analysis. Specifically, the pre-amplification analyzer can transport the consumables 3000 to be amplified to the forward transport position 41a of the installation cavity 11 through the forward transport mechanism 4a and the forward transport window 12a, so that the amplification analyzer 1000 can perform the amplification analysis of the consumables 3000 to be amplified together with the pre-amplification analyzer, increase the number of consumables that the detection system can complete the amplification analysis per unit time, and improve the amplification efficiency of the detection system. In some embodiments, the pre-amplification analyzer can also only complete the loading of the consumables 3000 to be amplified without performing the amplification analysis, and the current amplification analyzer 1000 completes the amplification analysis, which is also conducive to improving the loading efficiency of the consumables 3000.

[0093] In some embodiments, the amplification analyzer 1000 includes a consumables cache position 111c. When the amplification mechanism 3 is in a non-idle state, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified from the forward transport position 41a to the consumables cache position 111c for caching. When the amplification mechanism 3 is in an idle state, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified to the amplification mechanism 3 for amplification.

[0094] In some embodiments, the installation cavity includes a consumable cache position 111c and a backward transfer position 41b. The processor 1001 of the amplification analyzer 1000 calls the third online mode stored in the memory 1005. In the third online mode, the processor controls the scheduling mechanism 2 to extract the consumable 3000 to be amplified from the consumable cache position 111c and transfer it to the backward transfer position 41b, or the processor controls the scheduling mechanism 2 to directly extract the consumable 3000 to be amplified from the forward transfer position and transfer it to the backward transfer position 41b.

[0095] Specifically, when the processor 1001 controls the scheduling mechanism 2 to transfer the consumables 3000, it will confirm the operating state of the amplification mechanism to determine whether the amplification mechanism is in an idle state or a non-idle state. In some embodiments, the processor 1001 can determine whether the number of consumables 3000 on the amplification mechanism 3 is less than the total number that can be accommodated. If so, it is determined that the amplification mechanism 3 is in an idle state, otherwise it is determined that the amplification mechanism 3 is in a non-idle state. In some embodiments, the processor 1001 may also only be in an empty state when the amplification mechanism 3 is in an empty state, that is, whether the number of consumables 3000 on the amplification mechanism 3 is zero. If so, it is determined that the amplification mechanism 3 is in an idle state, otherwise it is determined that the amplification mechanism 3 is in a non-idle state.

[0096] Therefore, by setting the consumable cache position 111c, more consumables can be loaded or produced through the front cascade device 2000a. When the amplification mechanism 3 is in a non-idle state, the forward transport mechanism 4a can still transport the consumables and cache them in the consumable cache position 111c, which is beneficial to improving the consumable loading efficiency.

[0097] In some embodiments, at least a portion of the forward transport mechanism 4a extends in the first direction in the installation cavity 11, and the amplification mechanism 3 and the signal analysis mechanism 5 are arranged side by side in the first direction. The extension direction of the forward transport mechanism 4a in the installation cavity 11 is consistent with the arrangement direction of the amplification mechanism 3 and the signal analysis mechanism 5, which can reduce the space occupied by the forward transport mechanism 4a in the first direction, reduce the length of the installation cavity 11 in the first direction, and also shorten the movement stroke of the scheduling mechanism 2 in the first direction, thereby improving the scheduling efficiency of the scheduling mechanism 2 for the consumables 3000. The arrangement of the amplification mechanism 3 and the signal analysis mechanism 5 along the extension direction of the forward transport mechanism 4a can reduce the distance of the consumables 3000 from the amplification mechanism 3 to the signal analysis mechanism 5, shorten the time of the consumables 3000 from the amplification mechanism 3 to the signal analysis mechanism 5, and improve the scheduling efficiency of the consumables 3000.

[0098] Please refer to Figure 7 In some embodiments, the transfer window 12 is a backward transfer window 12b, and the transfer mechanism 4 is a backward transfer mechanism 4b. The amplification analyzer 1000 is connected to the post-amplification analyzer 2000b through the backward transfer window 12b. One end of the backward transfer mechanism 4b is set or extended in the installation cavity 11, and the other end passes through the backward transfer window 12b and is set or extended in the post-amplification analyzer 2000b. The backward transfer mechanism 4b is used to transfer the consumables in the amplification analyzer 1000 to the post-amplification analyzer 2000b, so that the post-amplification analyzer can perform amplification analysis on the consumables 3000 to be amplified together with the current amplification analyzer 1000, thereby increasing the number of consumables that can be amplified and analyzed per unit time of the detection system, and improving the amplification efficiency of the detection system.

[0099] Correspondingly, a rearward transfer position 41b for transferring the consumables 3000 to the rearward amplification analyzer 2000b is provided in the installation cavity 11. The rearward transfer position 41b and the rearward transfer mechanism 4b overlap at least partially in the installation cavity 11, and the rearward transfer mechanism 4b can transfer the consumables on the rearward transfer position 41b to the rearward amplification analyzer 2000b. In some embodiments, a consumable loading position 111a for loading consumables is provided in the installation cavity 11.

[0100] In some embodiments, the processor 1001 of the amplification analyzer 1000 calls the second online mode stored in the memory 1005. In the second online mode, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified in the consumable loading position 111a, and transfer it to the backward transport position 41b, so as to transport it to the post-amplification analyzer 2000b through the backward transport mechanism 4b, so that the post-amplification analyzer performs amplification analysis.

[0101] Specifically, the backward transport mechanism 4b can be pre-set in the amplification analyzer 1000; it can also be pre-set in the post-amplification analyzer 2000b; it can also be an independent component independent of the amplification analyzer 1000 and the post-amplification analyzer 2000b, and it is only assembled and installed when the amplification analyzer 1000 and the post-amplification analyzer 2000b are spliced. The present application does not impose any restrictions on this.

[0102] Please refer to Figure 1 In some embodiments, the shell 1 also includes a loading window 13, and the consumables 3000 to be amplified are placed into the consumable loading position 111a through the loading window 13, and the backward transfer window 12b and the loading window 13 are arranged on adjacent sides of the shell 1, which is conducive to reducing the volume of the amplification analyzer 1000 and improving the user experience.

[0103] In some embodiments, the backward transport mechanism 4b is controlled by the processor of the amplification analyzer 1000. Specifically, the processor controls the operation of the backward transport mechanism 4b and obtains the state of the backward transport mechanism 4b. For example, under the control of the processor of the amplification analyzer 1000, the scheduling mechanism 2 first transfers the consumables to the backward transport position 41b, and then controls the backward transport mechanism 4b to transfer the consumables to the rear amplification analyzer 2000b through the backward transport window 12b. In some embodiments, the backward transport mechanism 4b is controlled by the processor of the rear amplification analyzer 2000b. Specifically, the processor controls the operation of the backward transport mechanism 4b and obtains the state of the backward transport mechanism 4b. For example, under the control of the processor of the amplification analyzer 1000, the scheduling mechanism 2 will transfer the consumables to the rear transfer position 41b. After that, the processor of the amplification analyzer 1000 will send the status information of the scheduling mechanism 2 to the processor of the rear amplification analyzer 2000b through the information interaction mechanism between the processor of the rear amplification analyzer 2000b directly or indirectly (through the host computers of both), and finally, the processor of the rear amplification analyzer 2000b will control the rear transfer mechanism 4b to transfer the consumables to the rear amplification analyzer 2000b through the rear transfer window 12b. In some embodiments, the rear transfer mechanism 4b is controlled by the processor of the host computer of the amplification analyzer 1000 and the rear amplification analyzer 2000b. Specifically, the processor of the host computer directly or indirectly controls the work of the rear transfer mechanism 4b, obtains the state of the rear transfer mechanism 4b, and directly or indirectly controls the rear transfer mechanism 4b to transfer the consumables 3000 on the rear transfer position 41b according to the state.

[0104] In some embodiments, the installation cavity 11 further includes a consumables cache position 111c and / or a forward transfer position; the processor 1001 of the amplification analyzer 1000 calls the third online mode stored in the memory 1005. In the third online mode, the processor controls the scheduling mechanism 2 to extract the consumables to be amplified from one of the forward transfer position 41a and the consumables cache position 111c to the amplification mechanism 3 for amplification, and then transfers it to the signal analysis mechanism 5 to determine and output the corresponding analysis results. Alternatively, the processor controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified from the consumables cache position 111c to the backward transfer position 41b.

[0105] In some embodiments, please refer to Figure 8The transfer window 12 includes a forward transfer window 12a and a backward transfer window 12b. The amplification analyzer 1000 is connected to the front cascade device 2000a through the forward transfer window 12a. One end of the forward transfer mechanism 4a is set or extended in the installation cavity 11, and the other end passes through the forward transfer window 12a and is set or extended in the front cascade device 2000a. The amplification analyzer 1000 is connected to the backward amplification analyzer 2000b through the backward transfer window 12b. One end of the backward transfer mechanism 4b is set or extended in the installation cavity 11, and the other end passes through the backward transfer window 12b and is set or extended in the backward amplification analyzer 2000b.

[0106] Correspondingly, the installation cavity 11 is provided with a forward transfer position 41a and a backward transfer position 41b. At this time, the processor of the amplification analyzer 1000 calls the third online mode stored in the memory. In the third online mode, when the amplification analyzer 1000 meets the preset transportation condition, the processor controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified in the forward transfer position 41a, and directly transfers it to the backward transfer position 41b, so as to transport it to the rear amplification analyzer 2000b through the backward transfer mechanism 4b. Through the direct transportation of the amplification analyzer 1000, the rear amplification analyzer 2000b can directly perform amplification analysis on the consumables 3000 that have been completed by the pre-cascade device and have been prepared by droplets, which can meet the needs of specific scenarios, especially the situation where the droplet preparation efficiency is higher than the amplification analysis efficiency. Specifically, the preset transportation condition can be that the amplification mechanism 3 is in a non-idle state, or that the processor 1001 receives a user instruction, or that other preset scheduling conditions are met, and this application does not limit this.

[0107] In some embodiments, when the amplification mechanism 3 is in a non-idle state, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified from the forward transfer position 41a to the consumables cache position 111c for caching; when the amplification mechanism 3 is in an idle state, the scheduling mechanism 2 is controlled to extract the consumables 3000 to be amplified to the amplification mechanism 3 for amplification. In some embodiments, when the amplification mechanism 3 is in a non-idle state, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified from the forward transfer position 41a to the consumables cache position 111c for caching, and then the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified on the consumables cache position 111c to the backward transfer position 41b, so that the consumables to be amplified are transported to the post-amplification analyzer 2000b through the backward transfer position 41b.

[0108] In some embodiments, when the signal analysis mechanism 5 is in a non-idle state, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be analyzed for signal from the amplification mechanism 3 to the consumables cache position 111c for caching; when the signal analysis mechanism 5 is in an idle state, the scheduling mechanism 2 is controlled to extract the consumables 3000 to be analyzed for signal to the signal analysis mechanism 5 for analysis. In some embodiments, when the signal analysis mechanism 5 is in a non-idle state, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be analyzed for signal from the amplification mechanism 3 to the consumables cache position 111c for caching; thereafter, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be analyzed for signal on the consumables cache position 111c to the backward transfer position 41b, thereby transferring the consumables to be analyzed for signal to the post-amplification analyzer 2000b through the backward transfer position 41b for signal analysis.

[0109] In some embodiments, the processor 1001 can determine whether to perform the cache action by judging whether the number of consumables 3000 on the backward transfer position 41b is greater than the total number that can be accommodated or whether it is cleared. If so, the processor 1001 will control the scheduling mechanism 2 to extract the consumables 3000 to be amplified from the forward transfer position 41a to the consumable cache position 111c for cache, and then, when the backward transfer position 41b is idle, the scheduling mechanism 2 will be controlled to extract the consumables 3000 to be amplified to the backward transfer position 41b.

[0110] It should be noted that the forward transfer mechanism 4a can be fixed in the installation cavity 11 of the amplification analyzer 1000, and extend out of the installation cavity 11 and into the front cascade device 2000a through the forward transfer window 12a, and be arranged or extended in the front cascade device; of course, the forward transfer mechanism 4a can also be fixed in the front cascade device 2000a, and extend into the installation cavity 11 through the forward transfer window 12a, and be arranged or extended in the amplification analyzer 1000. Similarly, the backward transport mechanism 4b can be fixedly disposed in the installation cavity 11 of the amplification analyzer 1000, and extend out of the installation cavity 11 through the backward transport window 12b and extend into the rear amplification analyzer 2000b, and be disposed or extended in the rear amplification analyzer 2000b; the backward transport mechanism 4b can also be fixed in the rear amplification analyzer 2000b, and extend into the installation cavity 11 through the backward transport window 12b, and be disposed or extended in the amplification analyzer 1000, and the present application does not impose any restrictions on this.

[0111] Specifically, taking the case where a part of the backward transport mechanism 4b is fixed in the rear-end amplification analyzer 2000b as an example, when the amplification analyzer 1000 and the rear-end amplification analyzer 2000b are spliced, the other part of the backward transport mechanism 4b can be fixed in the installation cavity 11 through the backward transport window 12b, or it can only extend in the space of the installation cavity 11. In one example, the two parts of the backward transport mechanism 4b are detachable, and when the amplification analyzer 1000 and the rear-end amplification analyzer 2000b are spliced, the part of the backward transport mechanism 4b extending into the amplification analyzer 1000 is installed, which does not affect the use of both the amplification analyzer 1000 and the rear-end amplification analyzer 2000b in the stand-alone mode. Similarly, when the amplification analyzer 1000 and the front-end cascade device 2000a are connected, the forward transport mechanism 4a can refer to the design of the backward transport mechanism 4b.

[0112] It should be noted that the consumables buffer position 111c, the waste placement position 111b, and the consumables loading position 111a referred to above may be independent spaces spaced apart in the installation cavity 11, or may at least partially overlap in the space in the installation cavity. In one possible implementation of the present application, at least two of the consumables buffer position 111c, the waste placement position 111b, and the consumables loading position 111a are reused positions, that is, at least two of the consumables buffer position 111c, the waste placement position 111b, and the consumables loading position 111a overlap in space in the installation cavity 11. For example, the reuse of the consumables loading position 111a and the consumables buffer position 111c can improve the space utilization rate of the installation cavity 11.

[0113] In some embodiments, the amplification mechanism 3 and the signal analysis mechanism 5 are arranged side by side along the first direction, the consumable sample loading position 111a and the amplification mechanism 3 are arranged side by side along the second direction, and a first loading space extending along the first direction is provided in the installation cavity 11, and the first loading space is used for the installation of the backward transport mechanism 4b, wherein the first direction and the second direction are perpendicular to each other. By providing the first loading space extending along the first direction, the space occupied by the backward transport mechanism 4b in the installation cavity 11 in the first direction can be effectively reduced, the length of the installation cavity 11 in the first direction can be shortened, and the dispatching efficiency of the dispatching mechanism 2 can be improved.

[0114] In some embodiments, the forward transfer mechanism 4a extends in the installation cavity 11 along the first direction, and the extension portion is at least partially located between the consumables cache position 111c and the amplification mechanism 3; the consumables cache position 111c and the amplification mechanism 3 are arranged side by side along the second direction, wherein the first direction and the second direction are perpendicular to each other. By arranging the forward transfer mechanism 4a at the consumables cache position 111c and the amplification mechanism 3, the distance of the scheduling mechanism 2 from the forward transfer position 41a to the consumables cache position 111c, or from the forward transfer position 41a to the amplification mechanism 3 to schedule the consumables 3000 can be reduced, and the scheduling time of the scheduling mechanism 2 between the forward transfer position 41a and the consumables cache position 111c, or the forward transfer position 41a or the amplification mechanism 3 can be reduced, thereby improving the scheduling efficiency of the scheduling mechanism 2. Similarly, the relative arrangement of the consumable cache position 111c and the amplification mechanism 3 in the second direction can also reduce the distance of the consumable cache position 111c to dispatch the consumable 3000 to the amplification mechanism 3, reduce the scheduling time of the scheduling mechanism 2 between the consumable cache position 111c and the amplification mechanism 3, and improve the scheduling efficiency of the scheduling mechanism 2.

[0115] In some embodiments, in order to improve the reliability of the detection system, before starting / activating the transport mechanism 4, the processor 1001 will also detect the online status of the amplification analyzer 1000 and the front cascade device 2000a, or the amplification analyzer 1000 and the rear amplification analyzer 2000b, or the amplification analyzer 1000 and the front cascade device 2000a and the rear amplification analyzer 2000b. The online status includes: whether the amplification analyzer 1000, the front cascade device 2000a, and the rear amplification analyzer 2000b are mechanically spliced ​​with each other; whether the amplification analyzer 1000, the front cascade device 2000a, and the rear amplification analyzer 2000b have established an effective connection in communication; whether the forward transport mechanism 4a and the rear transport mechanism 4b can travel smoothly between the amplification analyzer 1000, the front cascade device 2000a, and the rear amplification analyzer 2000b, etc. When the above connection states are all kept normal, the transfer mechanism 4 is controlled to exchange information, otherwise, the transfer mechanism is prohibited from working, and the user is reminded that the connection between the amplification analyzer 1000 and the front cascade device 2000a, or the amplification analyzer 1000 and the rear amplification analyzer 2000b is abnormal. In this way, the amplification analyzer 1000 is prevented from idling or being blocked due to the amplification analyzer 1000 not being connected to the front cascade device 2000a or the connection being abnormal, thereby improving the reliability of the detection system operation.

[0116] In some embodiments, please refer to Fig. 9, the working modes stored in the memory include stand-alone mode. In stand-alone mode, the user will first place the consumable 3000 to be amplified on the consumable loading position 111a. The processor 1001 will set the consumable loading position 111a as the first placement position and the waste placement position 111b as the second placement position. Afterwards, the processor 1001 controls the scheduling mechanism 2 to extract the consumable 3000 to be amplified from the consumable loading position 111a, and transfer it to the amplification mechanism 3 for amplification, and then transfer it to the signal analysis mechanism 5 for signal analysis to determine and output the corresponding analysis results, and finally transfer the consumable 3000 that has completed amplification to the waste placement position 111b, thereby completing the amplification and analysis of the consumable 3000.

[0117] The transfer mechanism 4 can be a manipulator, a conveyor belt, or a mechanical mobile platform, and this application does not limit this. Figure 2 and Fig.10 In some embodiments, the transfer mechanism 4 includes a guide rail 42, a mobile platform 43, and a drive assembly 44. The guide rail 42 is disposed in the installation cavity 11, and the mobile platform 43 is slidably disposed on the guide rail 42. The drive assembly 44 is used to drive the mobile platform 43 to slide along the guide rail 42. The drive assembly can be a motor or a cylinder, and the present application does not limit this.

[0118] In actual application, when the amplification analyzer 1000 and the cascade device 2000 are spliced, the guide rail 42 is provided between the installation cavity 11 of the amplification analyzer 1000 and the cascade device 2000 through the transfer window. The mobile platform 43 can slide along the guide rail 42 under the drive of the driving component 44, so as to transfer the consumables in the installation cavity 11 to the cascade device 2000, or transfer the consumables in the cascade device 2000 to the installation cavity 11. In some embodiments, the guide rail 42 can be provided only in one of the amplification analyzer 1000 and the cascade device 2000, but the mobile platform 43 is in a part of the guide rail 42 (for example, the farthest end), and the mobile platform 43 extends inside the other one of the amplification analyzer 1000 and the cascade device 2000.

[0119] In some application scenarios, due to the layout of the device itself, the scheduling mechanism in the amplification analyzer 1000 and the scheduling mechanism in the cascade device 2000 may schedule consumables in different directions. Figure 3 For example, Figure 3 The scheduling mechanism 2 of the amplification analyzer 1000 schedules the consumables along the second direction Y, while the scheduling mechanism in the cascade device 2000 schedules the consumables along the first direction Y. In order to facilitate the scheduling mechanisms of different devices to schedule the consumables on the mobile platform 43, please refer to Fig.11In one embodiment, the mobile platform 43 includes a loading seat 431 and a rotating seat 432. The loading seat is slidably disposed on the guide rail 42, and the rotating seat is rotatably disposed on the loading seat. The rotating seat has a first position extending along a first direction and a second position extending along a second direction relative to the device seat. When the mobile platform 43 is located in the amplification analyzer 1000, the rotating seat 432 is located in the second position, and when the mobile platform 43 is located in the cascade device 2000, the rotating seat 432 is located in the first position. In this way, the scheduling direction between the various devices is coordinated to realize the flexible turning of the consumables, and the scheduling fluency of the scheduling mechanism for the consumables is improved.

[0120] Specifically, the transfer mechanism includes a reversing component, which is used to drive the rotating seat to switch between the first position and the second position. The reversing component can be a motor, a rotary cylinder, or other rotary drive components, which is not limited in this application. Fig.12 and Fig.13 In one embodiment, the reversing assembly 433 includes a push member 4331 and an elastic member 4332. The push member 4331 is disposed on the motion path of the mobile platform 43. The push member 4331 is configured to contact the rotating seat 432 when the rotating seat 432 reaches a set position (for example, reaches the farthest position of the guide rail), and push the rotating seat 432 to rotate from the second position to the first position. The elastic member 4332 is disposed between the rotating seat 432 and the loading seat 431. The elastic member 4332 is configured to drive the rotating seat 432 to reset from the first position to the second position when the push member 4331 is separated from the rotating seat 432, thereby realizing unpowered reversing.

[0121] In specific applications, such as Fig.13 As shown, in the initial state of the amplification analyzer 1000, the rotating seat 432 is in the second position. Fig.14 When the mobile platform 43 moves along the guide rail 42, the rotating seat 432 will contact the push member 4331 and rotate from the second position to the first position under the push of the push member 4331. Fig.15 As shown, when the mobile platform 43 reaches the preset position, the rotating seat 432 contacts the limiting member 4333 and is fixed at the first position under the limiting of the limiting member 4333 and the pushing member 4331. When the mobile platform 43 leaves the amplification analyzer 1000, the rotating seat 432 separates from the pushing member 4331, and then the rotating seat 432 returns from the first position to the second position under the action of the elastic member 4332, thereby achieving reversal.

[0122] It should be noted that in some embodiments, the preset position can be set before the forward transfer position or the backward transfer position, that is, the rotating seat 432 has been rotated to the first position before reaching the forward transfer position 41a or the backward transfer position 41b. In some embodiments, the preset position can also be set just at the forward transfer position or the backward transfer position, that is, when the rotating seat 432 moves to the forward transfer position 41a or the backward transfer position 41b, the rotating seat 432 is fixed to the first position by the push member 4331.

[0123] Please refer to Figure 3 The amplification analyzer 1000 also includes a liquid path mechanism 6, which is used to provide an injection channel and a sample oil carrier channel. The injection channel is responsible for introducing the sample to be analyzed into the amplification reaction system to ensure that the sample can smoothly enter the amplification reactor for polymerase chain reaction. The sample oil carrier channel is used to form a suitable environment to prevent the sample from evaporating or contaminating during the reaction process and to ensure the stability and accuracy of the reaction.

[0124] In some embodiments, the fluid path mechanism 6 and the signal analysis mechanism 5 are arranged side by side along the second direction, wherein the first direction and the second direction are perpendicular to each other. In this way, the space occupied by the fluid path mechanism 6 in the first direction of the installation chamber 11 is reduced, the distance between the forward transfer position 41a and the backward transfer position 41b is reduced, the movement time of the dispatching mechanism 2 between the forward transfer position 41a and the backward transfer position 41b is reduced, and the dispatching efficiency of the dispatching mechanism 2 is improved.

[0125] In some embodiments, a first loading space extending along the first direction is provided between the signal analysis mechanism 5 and the liquid circuit mechanism 6; the first loading space is used for at least partial installation of the transport mechanism (i.e., the backward transport mechanism 4b) in the rear amplification analyzer 2000b. By arranging the backward transport mechanism 4b along the first direction between the signal analysis mechanism 5 and the liquid circuit mechanism 6, the space occupied by the backward transport mechanism 4b in the installation chamber 11 in the first direction is reduced, the distance between the forward transport position 41a and the backward transport position 41b is reduced, the movement time of the dispatch mechanism 2 between the forward transport position 41a and the backward transport position 41b is reduced, and the dispatch efficiency of the dispatch mechanism 2 is improved.

[0126] Please refer to Fig.16 The present application also proposes a control method for an amplification analyzer, which is stored in a memory and executed by a processor to control the amplification analyzer to work. The control method includes:

[0127] S101, receiving user instructions.

[0128] S102, determining a working mode corresponding to the instruction according to the user instruction, and determining a first placement position for consumables to be amplified according to the corresponding working mode; and / or determining a second placement position for consumables that have completed amplification.

[0129] S103, the scheduling mechanism extracts the consumables to be amplified in the first placement position, transfers them to the amplification mechanism for amplification, and then transfers them to the signal analysis mechanism for signal analysis to determine and output the corresponding analysis results, and finally transfers the consumables that have completed amplification to the second placement position.

[0130] The instructions that the user can send to the processor include: at least two of the stand-alone mode instructions, the first online mode instructions, the second online mode instructions, and the third online mode instructions. The working mode includes: at least two of the stand-alone mode, the first online mode, the second online mode, and the third online mode. The first placement position includes at least one of the consumable loading position, the forward transfer position, and the consumable buffer position; the second placement position includes at least one of the waste placement position, the backward transfer position, or the consumable buffer position.

[0131] Please refer to Fig.17 , determining a working mode corresponding to the instruction according to the user instruction, and determining a first placement position of the consumable to be amplified according to the corresponding working mode; and / or determining a second placement position of the consumable that has completed amplification includes:

[0132] S1021. When receiving a stand-alone mode instruction, switch the working mode to the stand-alone mode, and set the consumable loading position to the first placement position, and set the waste placement position to the second placement position; and / or

[0133] S1022, when receiving the first online mode instruction, switching the working mode to the first online mode, and setting the forward transfer position as the first placement position, and setting the waste placement position or the consumables buffer position as the second placement position; and / or

[0134] S1023, when receiving the second online mode instruction, switching the working mode to the second online mode, and setting the consumable loading position or the consumable buffer position as the first placement position, and setting the backward transfer position as the second placement position; and / or

[0135] S1024. When receiving the third online mode instruction, switch the working mode to the third online mode, set the forward transfer position as the first placement position, set the backward transfer position as the second placement position, and the processor controls the scheduling mechanism to extract the consumables to be amplified from the first placement position to the second placement position.

[0136] Please refer to Fig.18Specifically, in the third online mode, the processor controls the scheduling mechanism to extract the consumables to be amplified from the first placement position to the second placement position, which may include the following:

[0137] S10241. When the amplification analyzer meets the preset transfer conditions, the processor controls the scheduling mechanism to extract the consumables to be amplified in the forward transfer position and directly transfer them to the backward transfer position.

[0138] S10242. When the amplification mechanism is in a non-idle state, the processor controls the scheduling mechanism to extract the consumables to be amplified from the forward transfer position and cache them in the consumable cache position; when the amplification analyzer meets the preset transfer conditions, the processor controls the scheduling mechanism to extract the consumables to be amplified from the consumable cache position and directly transfer them to the backward transfer position.

[0139] S10243. When the signal analysis mechanism is in a non-idle state, the processor controls the scheduling mechanism to extract the consumables to be analyzed for signal from the amplification mechanism to the consumable cache for caching; when the signal analysis mechanism is in an idle state, the processor controls the scheduling mechanism to extract the consumables to be analyzed for signal to the signal analysis mechanism for analysis.

[0140] Therefore, the amplification analyzer of the embodiment of the present application can select different working modes according to different scene requirements, and determine the corresponding first placement position and second placement position according to different working modes. The processor can control the scheduling mechanism to extract the consumables at the first placement position and place them on the second placement position. By selecting different first placement positions and second placement positions, the amplification analyzer can not only complete the single-machine analysis of consumables, but also can be cascaded with other devices with the cooperation of the transfer window and the transfer mechanism, so as to expand the use scenarios of the amplification analyzer and improve the flexibility of the use of the amplification analyzer.

[0141] It should be noted that in this application Figure 6-Figure 9 The dispatching mechanism is for illustration only and does not represent the specific location of the dispatching mechanism of this equipment.

[0142] It should be noted that in the present application, the processor can be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc., and the present application does not impose any restrictions on this.

[0143] The memory stores an operating system, a network communication module, a user interface module, and an amplification analyzer control program. The memory may be a volatile memory or a nonvolatile memory, or may include both volatile and nonvolatile memories. Among them, the nonvolatile memory may be a read-only memory (ROM), a programmable read-only memory (PROM), an erasable programmable read-only memory (EPROM), an electrically erasable programmable read-only memory (EEPROM), or a flash memory. The volatile memory may be a random access memory (RAM), which is used as an external cache. By way of example but not limitation, many forms of RAM are available, such as static random access memory (SRAM), dynamic random access memory (DRAM), synchronous dynamic random access memory (SDRAM), double data rate synchronous dynamic random access memory (DDR SDRAM), enhanced synchronous dynamic random access memory (ESDRAM), synchronous link DRAM (SLDRAM) and direct RAM bus random access memory (DR RAM).

[0144] In the description of the embodiments of the present application, it should be noted that the orientation or positional relationship of terms such as "center", "up", "down", "left", "right", "vertical", "horizontal", "inside" and "outside" are based on the orientation or positional relationship described in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0145] What is disclosed above is only a preferred embodiment of the present application, and it certainly cannot be used to limit the scope of rights of the present application. Ordinary technicians in this field can understand that all or part of the processes of implementing the above embodiment and equivalent changes made according to the claims of the present application are still within the scope covered by the present application.

Claims

1. An amplification analyzer, characterized in that: The amplification analyzer comprises a housing, a processor, a scheduling mechanism, an amplification mechanism, a signal analysis mechanism and a forward transport mechanism. The housing comprises an installation cavity and a forward transport window. The forward transport window is connected to the installation cavity. The scheduling mechanism, the amplification mechanism and the signal analysis mechanism are arranged in the installation cavity and are all controlled by the processor. A forward transport position is arranged in the installation cavity. The forward transport mechanism is used to transport the consumables to be amplified to the forward transport position through the forward transport window; The processor is configured to control the scheduling mechanism to extract the consumables to be amplified from the forward transport position, transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism for signal analysis to determine and output corresponding analysis results.

2. The amplification analyzer according to claim 1, characterized in that: The amplification analyzer is used to connect with the front cascade device; One end of the forward transfer mechanism is disposed or extended inside the front cascade device, and the other end of the forward transfer mechanism is disposed or extended inside the installation cavity.

3. The amplification analyzer according to claim 1 or 2, characterized in that: The amplification analyzer is also used to connect with a subsequent amplification analyzer; the shell also includes a rearward transport window for allowing the transport mechanism in the subsequent amplification analyzer to pass through and extend into the installation cavity.

4. The amplification analyzer according to claim 1, characterized in that: The amplification analyzer also includes a consumables cache location; The processor is configured to control the scheduling mechanism to extract the consumables to be amplified from the forward transfer position to the consumables cache position for caching when the amplification mechanism is in a non-idle state; When the amplification mechanism is in an idle state, the scheduling mechanism is controlled to extract the consumables to be amplified to the amplification mechanism for amplification.

5. The amplification analyzer according to claim 4, characterized in that: The forward transport mechanism extends in the installation cavity along a first direction, and the extension portion is at least partially located between the consumables buffer position and the amplification mechanism; The consumable material buffer position and the amplification mechanism are arranged side by side along a second direction, wherein the first direction and the second direction are perpendicular to each other.

6. The amplification analyzer according to claim 1, characterized in that: At least a portion of the forward transport mechanism extends in the installation cavity along a first direction, and the amplification mechanism and the signal analysis mechanism are arranged side by side along the first direction.

7. The amplification analyzer according to claim 6, characterized in that: The amplification analyzer further comprises a liquid path mechanism, which is used to provide an injection channel and a sample oil-carrying channel; the liquid path mechanism and the signal analysis mechanism are arranged side by side along a second direction, wherein the first direction and the second direction are perpendicular to each other.

8. The amplification analyzer according to claim 7, characterized in that: A first loading space extending along a first direction is provided between the signal analysis mechanism and the liquid path mechanism; the first loading space is used for at least partial installation of a transport mechanism in a post-amplification analyzer.

9. The amplification analyzer according to claim 1, characterized in that: The forward transport mechanism comprises: A guide rail, the guide rail is arranged in the installation cavity and extends out of the installation cavity through the forward transfer window; A loading seat, the loading seat being slidably disposed on the guide rail; A rotating seat, the rotating seat is rotatably disposed on the loading seat, and has a first position extending along the first direction and a second position extending along the second direction relative to the loading seat, the first direction and the second direction are two perpendicular directions, and the consumables to be amplified are placed on the rotating seat; and A reversing assembly is used to drive the rotating seat to rotate between the first position and the second position.

10. The amplification analyzer according to claim 9, characterized in that: The reversing assembly includes a push member and an elastic member, wherein the push member is arranged on the movement path of the loading seat; the push member is configured to contact the rotating seat to push the rotating seat to rotate from the second position to the first position; The elastic member is disposed between the rotating seat and the loading seat, and the elastic member is configured to drive the rotating seat to return from the first position to the second position when the push member is separated from the rotating seat.

11. An amplification analyzer, characterized in that: The amplification analyzer comprises a housing, a processor, a scheduling mechanism, an amplification mechanism and a signal analysis mechanism, the housing comprises an installation cavity and a rearward transport window, the rearward transport window is in communication with the installation cavity, the installation cavity comprises a consumable loading position for loading consumables to be amplified, the scheduling mechanism, the amplification mechanism and the signal analysis mechanism are arranged in the installation cavity and are all controlled by the processor; The backward transfer window is used for allowing the backward transfer mechanism to pass through; The processor is configured to control the scheduling mechanism to extract the consumables to be amplified from the consumable loading position, transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism for signal analysis to determine and output the corresponding analysis results; and the processor also controls the scheduling mechanism to extract the consumables to be amplified from the consumable loading position, transfer them to the backward transfer position of the backward transfer mechanism, so that the backward transfer mechanism drives the consumables to be amplified through the backward transfer window and transports them to the post-amplification analyzer.

12. The amplification analyzer according to claim 11, characterized in that: The processor is configured to, when the amplification mechanism is in a non-idle state, control the scheduling mechanism to extract the consumables to be amplified from the consumable loading position and transfer them to the backward transfer position of the backward transfer mechanism, either according to preset scheduling conditions or according to user instructions.

13. The amplification analyzer according to claim 11, characterized in that: The housing further comprises a loading window, and the consumables to be amplified are placed into the consumable loading position through the loading window; The backward transfer window and the loading window are arranged on adjacent sides of the shell.

14. The amplification analyzer according to claim 11, characterized in that: The amplification mechanism and the signal analysis mechanism are arranged side by side along a first direction, the consumables loading position and the amplification mechanism are arranged side by side along a second direction, and a first loading space extending along the first direction is arranged in the installation cavity, and the first loading space is used for the installation of the backward transfer mechanism, wherein the first direction and the second direction are perpendicular to each other.

15. The amplification analyzer according to claim 14, characterized in that: The amplification analyzer also includes a liquid circuit mechanism, which is used to provide an injection channel and a sample oil carrier channel; the liquid circuit mechanism and the signal analysis mechanism are arranged side by side along the second direction, and / or the first loading space is located between the liquid circuit mechanism and the signal analysis mechanism.

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