Application of Roman chamomile essential oil in removal of peculiar smell of shade-dried fabric

Abstract

According to the application of the Roman chamomile essential oil to removal of the peculiar smell of the fabric dried in the shade, the Roman chamomile essential oil is obtained through a steam distillation method and can effectively inhibit breeding of moraxella osladensis, and then the peculiar smell of the fabric dried in the shade is removed. The concentration of the Roman chamomile essential oil dissolved in the TSB culture medium is 80-160mL / mL, the Roman chamomile essential oil is cultured in a constant-temperature incubator at 37 DEG C for 24 hours, and the number of florae can be controlled within 1-10; along with the increase of the dosage of the Roman chamomile essential oil, the colony count and peculiar smell of the shade-dried fabric can be completely inhibited. The Roman chamomile essential oil effectively solves the problem of peculiar smell of wet or shade-dried fabrics, and has the advantages of being natural, environmentally friendly, small in damage to the fabrics and the like.

Description

Technical Field

[0001] The invention relates to the technical field of fabric odor treatment, and in particular to application of Roman chamomile essential oil in removing odor from shade-dried fabrics. Background Art

[0002] When the rainy season comes in southern my country, people often cannot see the sun for several days in a row, which makes the fabrics just washed become a "paradise" for the reproduction of various bacteria, fungi and other microorganisms. These microorganisms will produce metabolites during their growth, which will emit unpleasant odors, which not only affect the wearing experience of the fabrics, but also pose a potential threat to human health. In particular, a bacterium called "Oslo Moraxella" is different from other common bacteria. It is an aerobic bacterium and has good resistance to drying and UV. In the rainy season, even if you are very lucky to see the sun from time to time, bacteria such as Escherichia coli and Staphylococcus aureus can be killed, but Oslo Moraxella can still survive. Even if the clothes are finally dried in the shade, the humidity of the fabric is reduced, and most bacteria and molds have stopped growing, but Oslo Moraxella can still survive. The substances it metabolizes, such as "intermediate alcohols, aldehydes, acids", etc., especially some saturated acids, are the main source of the odor of shade-dried fabrics. Traditional methods of removing odors have limitations. For example, chemical deodorants may leave harmful substances, and physical adsorbents have limited deodorizing effects and need to be replaced frequently. Roman chamomile essential oil can inhibit bacterial growth by destroying the integrity of cell structure, affecting cell nucleic acid metabolism and protein synthesis, and inhibiting microbial energy and respiratory metabolism. It has good antibacterial properties, and its natural ingredients are expected to provide an effective way to solve this problem. Summary of the invention

[0003] The present invention aims to provide an application of Roman chamomile essential oil in removing the odor of shade-dried fabrics, wherein the odor of the shade-dried fabrics is mainly produced by microorganisms such as Moraxella osloensis; the Roman chamomile essential oil can inhibit the growth of Moraxella osloensis, thereby achieving the removal of the odor of the fabrics after the fabrics are shade-dried.

[0004] An application of Roman chamomile essential oil in removing odor from shade-dried fabrics, wherein the preparation method of the Roman chamomile essential oil comprises the following steps: S1. The Roman chamomile flower sample was crushed and passed through a 60-mesh sieve to obtain Roman chamomile powder; S2. Weigh 50.0 g of Roman chamomile flower powder, place it in a 500 mL round-bottom flask, add 300 mL of distilled water, soak for a certain period of time, and then connect the essential oil extractor and reflux condenser; S3. Add distilled water to the essential oil extractor until it reaches the mark. When a small amount of distilled water overflows into the flask, add 1 mL of n-hexane. S4. Then, the round-bottom flask was placed in an electric heating mantle and heated to 100°C for 5 minutes. The temperature of the electric heating mantle was adjusted from 100°C to 90°C. After distillation at 90°C for 4 hours, the heating was stopped. After the device was cooled for a while, the piston at the lower end of the essential oil extractor was opened to slowly release the water and collect the upper essential oil. S5. Finally, the collected distillation product was dehydrated with anhydrous sodium sulfate, and the n-hexane was evaporated in a 60°C water bath for 6 hours to obtain Roman chamomile essential oil.

[0005] Furthermore, the prepared Roman chamomile essential oil is collected in a container, and several volatilization sticks are inserted to facilitate the slow and uniform volatilization of the volatile oil. The container is placed in the space environment where the shade-dried fabric is located, such as in a closet, near the indoor drying fabric, etc. The amount of chrysanthemum volatile oil used is determined according to the size of the space and the degree of odor, and 3-5 mL of chrysanthemum volatile oil is used per cubic meter of space. The chrysanthemum volatile oil evaporates and contacts the microorganisms on the surface of the shade-dried fabric, inhibiting the growth and reproduction of Moraxella osloensis microorganisms, thereby reducing the production of its metabolites, thereby achieving the effect of inhibiting odor. At the same time, the chrysanthemum fragrance of the chrysanthemum volatile oil itself can cover up the odor of the fabric and improve the odor environment around the fabric.

[0006] Beneficial effects of the present invention: The Roman chamomile essential oil of the invention is mainly used for removing odor from shade-dried fabrics. The Roman chamomile essential oil has significant innovation and practicality. The concentration of the Roman chamomile essential oil dissolved in TSB culture medium is 80-160 mL / mL, and the bacteria are cultured in a 37°C constant temperature incubator for 24 h, and the bacterial population can be controlled within 1-10. With the increase in the dosage of the Roman chamomile essential oil, the colony count and odor of the shade-dried fabric are gradually suppressed, and the concentration of 8 mL / m 3 The Roman chamomile essential oil can completely inhibit the colony count and odor of shade-dried fabrics. The Roman chamomile essential oil of the invention effectively solves the odor problem of damp or shade-dried fabrics, and has the advantages of being natural, environmentally friendly, and having little damage to fabrics. DETAILED DESCRIPTION Example 1

[0007] S1. The Roman chamomile flower sample was crushed and passed through a 60-mesh sieve to obtain Roman chamomile powder; S2. Weigh 50.0 g of Roman chamomile flower powder, place it in a 500 mL round-bottom flask, add 300 mL of distilled water, soak for a certain period of time, and then connect the essential oil extractor and reflux condenser; S3. Add distilled water to the essential oil extractor until it reaches the mark. When a small amount of distilled water overflows into the flask, add 1 mL of n-hexane. S4. Then, the round-bottom flask was placed in an electric heating mantle and heated to 100°C for 5 minutes. The temperature of the electric heating mantle was adjusted from 100°C to 90°C. After distillation at 90°C for 4 hours, the heating was stopped. After the device was cooled for a while, the piston at the lower end of the essential oil extractor was opened to slowly release the water and collect the upper essential oil. S5. Finally, the collected distillation product was dehydrated with anhydrous sodium sulfate, and the n-hexane was evaporated in a 60°C water bath for 6 hours to obtain Roman chamomile essential oil. Example 2

[0008] Moraxella osloensis antibacterial experiment: Use sterile TSB medium to culture Oslo Moraxella to the middle of logarithmic growth, and determine the growth of typical Oslo Moraxella colonies in the field of vision through microscopic observation, and count them. Data record: 1 to 10 bacterial colonies in the field of vision are recorded as +, 10 to 100 bacterial colonies are ++, 100 to 1000 bacterial colonies are +++, and ++++ cannot be counted. Take the above cultured bacterial colonies, set up an experimental group (TSB medium dissolved and added with Roman chamomile essential oil extracted in Example 1, with concentrations of 20, 40, 80, and 160 mL / mL respectively) and a negative control group (TSB medium without Roman chamomile essential oil), dilute the bacterial culture liquid with the culture medium of the experimental group and the control group at different ratios (dilution ratio), place it in a 37°C constant temperature incubator for 24 h, and observe the growth of Staphylococcus epidermidis colonies in the culture medium through a microscope. The specific results are shown in Table 1 below (Note: positive +; negative -): Table 1 Effects of different concentrations of essential oils on Moraxella osloensis Example 3

[0009] Fabric Experiment: 1. Experimental Grouping Pre-experimental treatment: prepare a number of identical fabric samples that have undergone the same shade-drying treatment and have produced shade-dried odor, a number of identical fabric samples that have not undergone the shade-drying treatment and have not produced shade-dried odor, and a number of sealed glass boxes with the same volume of 1 cubic meter.

[0010] Control group: Take the same number of pieces of fabrics that produce odor when dried in the shade and place them in one of the sealed glass boxes without adding volatile oil.

[0011] Experimental group: Take the same number of pieces of fabrics that produce odor and place them in the remaining 5 1 m 3 In a sealed glass box, volatilization containers containing 0.5 mL, 1 mL, 2 mL, 4 mL and 8 mL of Roman chamomile essential oil were placed respectively.

[0012] Blank group: Take the same number of fabrics that have not been shade-dried and do not produce shade-dried odor and place them in one of the sealed glass boxes without adding volatile oil.

[0013] The fabrics in the control group, experimental group and blank group were placed in a sealed glass box for 12 hours before being taken out.

[0014] 2. Antibacterial test: All fabric samples of the control group, experimental group and blank group were cut into 5mm×5mm pieces, and 0.55±0.05g was accurately weighed as the test sample. Then, the samples were wrapped and numbered with small pieces of paper that had been sterilized in advance (the small pieces of paper and related contact materials were sterilized and sampled according to the national standard method of GB / T 20944.3). For each sample, 3 parallel samples were prepared for subsequent testing. The details are shown in Table 2.

[0015] Then add 70mL of 0.03mol / L PBS buffer solution to each of the prepared samples, and then place them at 25℃ for constant temperature shaking for 1 minute. After the shaking is over, take 1mL of sample for testing. Transfer 1mL of sample solution into the culture dish, then pour the nutrient agar medium, wait for it to solidify at room temperature, turn the culture dish upside down, and place it in a 37℃ incubator for 24 to 48 hours. During this period, record the number of colonies on the plate, and finally take the average of three plates for the number of colonies for each sample. If there are too many colonies to record, dilute the sample in the early stage. The specific method is to add 1mL of sample solution to 9mL of PBS buffer for dilution, and dilute it step by step in this way until the number of colonies can be clearly read.

[0016] 3. Shade-drying odor test: Refer to the technical requirements for ecological textiles GB / T18885 for testing. Sampling is the same as above, with a size ≥20cm×20cm; requirements for olfactory personnel: no smoking or eating spicy food beforehand, no makeup: frequent rest is required during the measurement. ① The test should be completed within 24 hours after obtaining the sample; ② Place the sample on the test bench, the operator should wash his hands in advance, wear gloves, hold the sample close to the nose with both hands, and smell the odor carefully. Sweat odor is recorded as having an odor, and the evaluation level is evaluated by the number of + to evaluate the intensity. No special odor is recorded as no odor, and recorded as -. The final odor level of each sample is the average of the odor levels given by the test participants. See Table 2 for details: Table 2 Effects of different amounts of Roman chamomile essential oil on bacterial colonies and odor on fabrics

[0017] The results show that the Roman chamomile essential oil has a good effect in inhibiting the growth of Moraxella osloensis after exercise, thereby reducing or removing the shade-dried odor of fabrics.

Claims

1. An application of Roman chamomile essential oil in removing odor from shade-dried fabrics, characterized in that: The odor of the wet fabric is mainly produced by microorganisms such as Moraxella osloensis; the Roman chamomile essential oil can inhibit the growth of Moraxella osloensis, thereby removing the odor of the fabric after it is dried in the shade.

2. The use of Roman chamomile essential oil in removing odor from shade-dried fabrics according to claim 1, characterized in that: The preparation method of the Roman chamomile essential oil comprises the following steps: S1. The Roman chamomile flower sample was crushed and passed through a 60-mesh sieve to obtain Roman chamomile powder; S2. Weigh 50.0 g of Roman chamomile flower powder, place it in a 500 mL round-bottom flask, add 300 mL of distilled water, soak for a certain period of time, and then connect the essential oil extractor and reflux condenser; S3. Add distilled water to the essential oil extractor until it reaches the mark. When a small amount of distilled water overflows into the flask, add 1 mL of n-hexane. S4. Then, the round-bottom flask was placed in an electric heating mantle and heated to 100°C for 5 minutes. The temperature of the electric heating mantle was adjusted from 100°C to 90°C. After distillation at 90°C for 4 hours, the heating was stopped. After the device was cooled for a while, the piston at the lower end of the essential oil extractor was opened to slowly release the water and collect the upper essential oil. S5. Finally, the collected distillation product was dehydrated with anhydrous sodium sulfate, and the n-hexane was evaporated in a 60°C water bath for 6 hours to obtain Roman chamomile essential oil.

3. The use of Roman chamomile essential oil in removing odor from shade-dried fabrics according to claim 1 or 2, characterized in that: Collect the prepared Roman chamomile essential oil in a container and insert several volatilization sticks to allow the volatile oil to evaporate slowly and evenly. Place the container in the space where the fabric is dried in the shade, such as in a closet or near the fabric drying indoors. Determine the amount of chrysanthemum volatile oil used according to the size of the space and the degree of odor, and use 3-5 mL of chrysanthemum volatile oil per cubic meter of space. The chrysanthemum volatile oil evaporates and contacts the microorganisms on the surface of the shade-dried fabric, inhibiting the growth and reproduction of Moraxella osloensis microorganisms, thereby reducing the production of its metabolites and achieving the effect of inhibiting odor. At the same time, the chrysanthemum fragrance of the chrysanthemum volatile oil itself can cover up the odor of the fabric and improve the odor environment around the fabric.

Images