Liquid sampling device for itaconic acid fermentation processes

Abstract

The application discloses a liquid sampling device for itaconic acid fermentation process, which comprises a sampling tube, an operating rod movably inserted into the sampling tube, a suspension sampling unit arranged on the sampling tube in sliding mode, and a bottom liquid sampling unit detachably installed at the lower end of the operating rod.

Description

Technical Field

[0001] This invention relates to a liquid sampling device for itaconic acid fermentation process. Background Technology

[0002] Itaconic acid, also known as methylene succinic acid or methylene succinic acid, is an unsaturated dicarboxylic organic acid. It contains unsaturated double bonds, exhibits highly reactive chemical properties, and can undergo polymerization between itself and with other monomers such as acrylonitrile. It is soluble in water, ethanol, and other solvents. It can undergo various addition, esterification, and polymerization reactions, making it an important raw material in the chemical synthesis industry and in chemical production.

[0003] Fermentation is a crucial step in the preparation of itaconic acid. During the fermentation process, regular sampling and testing are required according to the fermentation cycle to ensure the effectiveness of the fermentation.

[0004] The conventional sampling method involves inserting a sampling tube directly into the fermenter. However, during this process, a large amount of the upper suspension enters the sampling tube first, resulting in a sample that is not sufficiently representative.

[0005] To obtain the most accurate fermentation data, it is necessary to take separate samples of the upper suspension and the bottom liquid of the fermentation broth and then perform the tests.

[0006] However, existing sampling equipment is clearly unable to meet the requirements of simultaneous sampling, and samples are easily confused when there are many fermenters.

[0007] Based on the above problems, we designed a liquid sampling device for itaconic acid fermentation that can simultaneously extract both suspension and bottom liquid. Summary of the Invention

[0008] The technical problem to be solved by the present invention is to provide a liquid sampling device for itaconic acid fermentation process that can simultaneously extract suspension and bottom liquid.

[0009] To solve the above problems, the present invention adopts the following technical solution:

[0010] A liquid sampling device for itaconic acid fermentation includes a sampling tube and an operating rod that is movably inserted into the sampling tube. A suspension sampling unit is slidably disposed on the sampling tube, and a bottom liquid sampling unit is detachably installed at the lower end of the operating rod.

[0011] Preferably, a tube cap is detachably installed at the upper end of the sampling tube, the upper end of the operating rod passes upward through the tube cap, a limit ring is installed on the rod body of the operating rod, the limit ring limits the upward movement of the operating rod, a threaded portion is machined on the upper part of the operating rod, and a rod cap is fitted through the threaded portion, the rod cap limits the downward movement of the operating rod.

[0012] Preferably, a groove is machined on the upper part of the operating lever, and a scale is provided in the groove.

[0013] Preferably, the suspension sampling unit includes a first limiting ring and a sampling box. The first limiting ring limits the upward movement of the sampling box. An elastic retaining ring is embedded in the inner wall of the first limiting ring, and the elastic retaining ring is interference-fitted with the sampling tube. A hollow buoyancy chamber is injection-molded inside the sampling box. A first convex ring is injection-molded on the top of the sampling box, and a nut is secured through the first convex ring. A sampling chamber is injection-molded inside the sampling box, below the buoyancy chamber. A sampling hole is opened on the inner wall of the sampling box, corresponding to the upper part of the sampling chamber, and the sampling hole communicates with the sampling chamber. An inner tube is provided at the axis of the sampling box, and a first sealing ring is secured on the outer wall of the inner tube. A sealing ring is formed when the inner tube slides down the sampling box to seal the sampling hole. The upper part of the inner tube has an external thread, the diameter of which is larger than the inner diameter of the sampling box. After the inner tube slides down, the nut engages with the external thread, locking the height of the inner tube. A first sealing ring seals the sampling hole. A sliding cap engages with the external thread and transitions with the sampling tube. A second convex ring is located at the bottom of the sampling tube, limiting the downward movement of the sliding cap. The inner diameter of the inner tube is larger than the diameter of the second convex ring. After the connection between the external thread and the sliding cap is released, the sampling box is removed downward along the sampling tube.

[0014] Preferably, the inner wall of the first convex ring is injection molded to form an annular groove, and the outer wall of the nut is injection molded to form a retaining ring that fits into the groove, the retaining ring rotating along the groove.

[0015] Preferably, three or more sliding grooves are arranged in a ring on the inner wall of the sampling box, and an arc-shaped elastic sheet is provided at the lower part of the inner tube. The lower end of the elastic sheet is stuck in the sliding groove and can slide vertically along the sliding groove. When the external thread part is engaged with the nut, the elastic sheet is compressed and bulges up. The bulging elastic sheet supports the bottom liquid sampling unit. A convex tube part is injection molded at the bottom of the sampling box. Under the support of the convex tube part, the lower end of the bottom liquid sampling unit is lifted off the ground.

[0016] Preferably, the bottom liquid sampling unit includes a storage cup and a connecting cap. The upper end of the storage cup is machined with an insert end, which is inserted into the sampling tube. The storage cup has a storage chamber inside, and the upper end of the storage chamber is machined with an internal thread. The bottom of the connecting cap has a connecting portion, and the outside of the connecting portion is machined with an external thread that matches the internal thread. An inlet hole is machined near the top of the outer wall of the connecting portion, and a sealing gasket is fitted on the outer wall of the connecting portion. When the connecting portion moves along the internal thread... When rotated to its limit, the sealing gasket is sandwiched between the liquid storage cup and the connecting cap. At this time, the liquid inlet is closed. The top of the connecting cap is machined with a threaded hole that matches the operating rod. A first sealing gasket is clamped on the outer wall of the embedded end. As the operating rod moves upward, the first sealing gasket seals between the sampling tube and the liquid storage cup. The diameter of the liquid storage cup is smaller than the inner diameter of the inner tube body. The liquid storage cup is conical, and its diameter gradually decreases downward. After the liquid storage cup is removed, it is positioned by an elastic sheet.

[0017] Preferably, a second sealing ring is fitted on the outer wall of the connecting cap, and the second sealing ring is interference-fitted with the inner wall of the sampling tube.

[0018] Preferably, an annular slot is coaxially provided at the bottom of the connecting cap, and a clamping ring made of elastic rubber is inserted into the slot. An annular filter screen is clamped on the inner side of the clamping ring. The outer wall of the clamping ring is formed by interference fit with the inner wall of the sampling tube. The upper end of the embedded end is machined with a small diameter portion, which fits against the inner wall of the filter screen.

[0019] The beneficial effects of this invention are:

[0020] One advantage is that this device can sample the upper suspension of the fermentation broth through the suspension sampling unit and the bottom fermentation broth through the bottom liquid sampling unit. Two types of samples can be extracted in one operation, making sampling more efficient.

[0021] Secondly, after sampling is completed, the bottom liquid sampling unit can be inserted into the suspension sampling unit, so that samples from the same fermenter are placed together to avoid confusion.

[0022] Thirdly, when sampling, the bottom liquid sampling unit is in a closed state before the sampling depth is reached. Only when the sampling depth is reached will the bottom liquid sampling unit extend out of the sampling tube, thus making the sample more accurate.

[0023] Fourthly, the suspension sampling unit is suspended above the fermentation liquid, preventing the device from falling into the fermentation tank.

[0024] Fifthly, this device has a simple structure and low cost, making it suitable for widespread use. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 This is a schematic diagram of the structure of the present invention;

[0027] Figure 2 This is a cross-sectional view of the present invention;

[0028] Figure 3 An exploded view of the control lever and the bottom liquid sampling unit;

[0029] Figure 4 for Figure 3 Enlarged view at point A;

[0030] Figure 5 This is a 3D view of the sampling tube;

[0031] Figure 6 This is a schematic diagram showing the connection between the suspension sampling unit and the sampling tube.

[0032] Figure 7 A three-dimensional view of the suspension sampling unit;

[0033] Figure 8 for Figure 6 Enlarged view at point B;

[0034] Figure 9 for Figure 6 Enlarged view at point C;

[0035] Figure 10 This is a schematic diagram showing the combination of the suspension sampling unit and the bottom liquid sampling unit;

[0036] Figure 11 This is a cross-sectional view of the bottom liquid sampling unit;

[0037] Figure 12 This is an exploded view of the bottom liquid sampling unit.

[0038] Figure 13 for Figure 11 Enlarged view at point D. Detailed Implementation

[0039] All features disclosed in this specification, or all steps in all disclosed methods or processes, may be combined in any way, except for mutually exclusive features and / or steps.

[0040] Any feature disclosed in this specification (including any appended claims, abstract, and drawings) may be replaced by other equivalent or similar features for a similar purpose, unless specifically stated otherwise. That is, unless specifically stated otherwise, each feature is merely one example of a series of equivalent or similar features.

[0041] In the description of this invention, it should be understood that the terms "one end", "the other end", "outer side", "upper", "inner side", "horizontal", "coaxial", "center", "end", "length", "outer end", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing this invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0042] Furthermore, in the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0043] In this invention, unless otherwise explicitly specified and limited, the terms "set," "socket," "connect," "through," and "plug-in" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0044] See Figure 1 The device shown is a liquid sampling device for itaconic acid fermentation process, including a sampling tube 1 and an operating rod 2 that is movably inserted into the sampling tube 1. A suspension sampling unit 3 is slidably arranged on the sampling tube 1, and a bottom liquid sampling unit 4 is detachably installed at the lower end of the operating rod 2.

[0045] In the above technical solution, the sampling work can be completed simultaneously in one sampling process by the suspension sampling unit 3 and the bottom liquid sampling unit 4, thereby improving the sampling efficiency.

[0046] For multiple fermenters, multiple sets of suspension sampling units 3 and bottom liquid sampling units 4 can be prepared.

[0047] The suspension sampling unit 3 and the bottom sampling unit 4 of each group can be combined for easy classification and to avoid mistakes.

[0048] See Figures 2 to 5As shown, a tube cap 101 is detachably installed at the upper end of the sampling tube 1. The upper end of the operating rod 2 passes upward through the tube cap 101. A limit ring 21 is installed on the rod body of the operating rod 2. The limit ring 21 limits the upward movement of the operating rod 2. A threaded portion 22 is machined on the upper part of the operating rod 2. A rod cap 23 is fitted through the threaded portion 22. The rod cap 23 limits the downward movement of the operating rod 2.

[0049] In the above technical solution, when the rod cap 23 is screwed down along the threaded portion 22 to the limit position, the sliding of the operating rod 2 is limited by the limiting ring 21 and the rod cap 23.

[0050] At this time, the operating lever 2 moves upward to its limit position, and the bottom liquid sampling unit 4 and the sampling tube 2 cooperate to form a seal.

[0051] See Figure 4 As shown, a groove 24 is machined on the upper part of the operating lever 2, and a scale 2444 is provided in the groove 24.

[0052] The groove 24 and the scale 2444 facilitate marking the position of the rod cap 23.

[0053] See Figures 5 to 11As shown, the suspension sampling unit 3 includes a first limiting ring 31 and a sampling box 32. The first limiting ring 31 limits the upward movement of the sampling box 32. An elastic retaining ring 3112 is embedded in the inner wall of the first limiting ring 31, and the elastic retaining ring 3112 is press-fitted with the sampling tube 1. A hollow buoyancy chamber 33 is injection-molded inside the sampling box 32. A first convex ring 34 is injection-molded on the top of the sampling box 32, and a nut 35 is secured through the first convex ring 34. A sampling chamber 36 is injection-molded inside the sampling box 32, located below the buoyancy chamber 33. A sampling hole 361 is opened on the inner wall of the sampling box 32 at a position corresponding to the upper part of the sampling chamber 36. The sampling hole 361 communicates with the sampling chamber 36. An inner tube 37 is provided at the axis of the sampling box 32, and a first sealing ring 381 is secured on the outer wall of the inner tube 37. After the inner tube 37 slides down along the sampling box 32, it closes the sampling hole 361. The upper part of the inner tube 37 is provided with an external thread portion 371, the diameter of which is larger than the inner diameter of the sampling box 32. After the inner tube 37 slides down, the nut 35 engages with the external thread portion 371, and the height of the inner tube 37 is locked at this time. The first sealing ring 381 seals the sampling hole 361. A sliding cap 39 is engaged through the external thread portion 371. The sliding cap 39 transitionally engages with the sampling tube 1. A second convex ring 121 is provided at the bottom of the outer side of the sampling tube 1. The second convex ring 121 limits the downward movement of the sliding cap 39. The inner diameter of the inner tube 37 is larger than the diameter of the second convex ring 121. After the connection between the external thread portion 371 and the sliding cap 39 is released, the sampling box 32 is taken out downward along the sampling tube 1.

[0054] In the above technical solution, the upper suspension of the fermentation broth enters through the sampling hole 361. During sampling, the first limiting ring 31 pushes the sampling box 32 down by the downward pressure of the sampling tube 1, so that the upper suspension enters the sampling chamber 36 through the sampling hole 361.

[0055] While sampling the upper layer, the bottom liquid sampling unit 4 is moved downward by operating the handle rod 2, so that it is disengaged from the sampling tube 2, and the bottom fermentation liquid is sampled simultaneously.

[0056] After sampling is completed, the sampling box 32 can be directly removed by unscrewing the sliding cap 39 and moving downwards along the sampling tube 1.

[0057] When it is necessary to remove the bottom liquid sampling unit 4, the upper screw cap 23 needs to be screwed on so that the operating lever 2 can slide down a sufficient distance to remove the bottom liquid sampling unit 4.

[0058] Nut 35 can lock the inner tube 37, so that the first sealing ring 381 can always effectively seal the sampling hole 361.

[0059] See Figure 8 As shown, the inner wall of the first convex ring 34 is injection molded with an annular groove 341, and the outer wall of the nut 35 is injection molded with a retaining ring 351 that fits into the groove 341. The retaining ring 351 rotates along the groove 341.

[0060] The above technical solution mainly achieves the positioning of nut 35.

[0061] See Figure 6 , Figure 9 and Figure 10 As shown, three or more sliding grooves 3221 are arranged in a ring on the inner wall of the sampling box 32. An arc-shaped elastic sheet 3222 is provided at the lower part of the inner tube 37. The lower end of the elastic sheet 3222 is stuck in the sliding groove 3221 and can slide vertically along the sliding groove 3221. When the external thread portion 371 is engaged with the nut 35, the elastic sheet 3222 is compressed and bulges up. The bulging elastic sheet 3222 provides support for the bottom liquid sampling unit 4. A convex tube portion 3223 is injection molded at the bottom of the sampling box 32. Under the support of the convex tube portion 3223, the lower end of the bottom liquid sampling unit 4 is lifted off the ground.

[0062] In the above technical solution, the cooperation between the slide groove 3221 and the elastic plate 3222 can realize the positioning of the inner tube 37 and avoid the rotation of the inner tube 37, so as to better cooperate with the nut and bolt.

[0063] After the nut is tightened to lock the inner tube 37, the elastic sheet 3222 is squeezed and deformed elastically to better position the bottom liquid sampling unit 4.

[0064] See Figures 10 to 13As shown, the bottom liquid sampling unit 4 includes a liquid storage cup 401 and a connecting cap 402. The upper end of the liquid storage cup 401 is machined with an embedded end 4441, which is inserted into the sampling tube 1. A liquid storage chamber 403 is provided inside the liquid storage cup 401. An internal thread 441 is machined at the upper end of the liquid storage chamber 403. A connecting part 404 is provided at the bottom of the connecting cap 402. An external thread 442 that mates with the internal thread 441 is machined on the outside of the connecting part 404. An inlet hole 405 is machined near the top of the outer wall of the connecting part 404. A sealing gasket 406 fits on the outer wall of the connecting part 404. When the connecting part 404 moves along the internal thread 401... When the thread 441 is screwed down to its limit, the sealing washer 406 is sandwiched between the liquid storage cup 401 and the connecting cap 402. At this time, the liquid inlet 405 is closed. The top of the connecting cap 402 is machined with a threaded hole 407 that matches the operating rod 2. The first sealing washer 4442 is clamped on the outer wall of the embedded end 4441. As the operating rod 2 moves upward, the first sealing washer 4442 seals between the sampling tube 1 and the liquid storage cup 401. The diameter of the liquid storage cup 401 is smaller than the inner diameter of the inner tube body 37. The liquid storage cup 401 is conical, and its diameter gradually decreases downward. After the liquid storage cup 401 is removed, it is positioned by the elastic sheet 3222.

[0065] In the above technical solution, sampling can be completed by the cooperation of the liquid storage cup 401 and the connecting cap 402. During sampling, the liquid inlet 405 is exposed to the outside of the liquid storage chamber 403. When the operating rod 2 is pushed down to expose the liquid inlet 405 to the outside of the sampling tube 1, the fermented bottom liquid enters into the liquid storage chamber 403 through the liquid inlet 405 to complete the sampling.

[0066] The sealing gasket 406 is designed to prevent fermentation broth from entering the sampling tube 1 before the sampling tube 1 reaches the required depth.

[0067] See Figure 11 As shown, a second sealing ring 4222 is fitted on the outer wall of the connecting cap 402, and the second sealing ring 4222 is interference-fitted with the inner wall of the sampling tube 1.

[0068] During the sampling process, the sliding position of the operating rod 2 is restricted by the rod cap 23, so that the second sealing ring 4222 is always sealed between the connecting cap 402 and the sampling tube 1 during the sampling process, preventing a large amount of fermentation liquid from entering the inside of the sampling tube 1.

[0069] See Figure 11 , Figure 12 and Figure 13As shown, an annular slot 4223 is coaxially provided at the bottom of the connecting cap 402. An elastic rubber clamping ring 4224 is inserted into the slot 4223. An annular filter screen 4225 is clamped on the inner side of the clamping ring 4224. The outer wall of the clamping ring 4224 is formed by interference fit with the inner wall of the sampling tube 1. The upper end of the embedded end 4441 is machined with a small diameter portion 4443, which fits against the inner wall of the filter screen 4225.

[0070] In the above technical solution, after the connecting cap 402 is removed upwards, the filter screen 4225 can be pulled downwards directly, which facilitates the maintenance of the filter screen 4225. Furthermore, the filter screen 4225 can slide down along the small diameter portion 4443. Therefore, after sampling is completed, there is no need to remove the filter screen 4225. The connection cap 402 can be screwed down to complete the sealing of the liquid storage tank 403.

[0071] The particulate matter in the fermentation tank settles in the lower layer of the fermentation liquid. Therefore, by setting up a filter screen 4225, these particulate matter can be blocked, so that the device can effectively extract the fermentation liquid and avoid extracting a large amount of particulate matter.

[0072] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of this invention is defined by the appended claims and their equivalents.

Claims

1. A liquid sampling device for itaconic acid fermentation process, characterized in that: Includes a sampling tube (1) and an operating rod (2) that is movably inserted into the sampling tube (1). A suspension sampling unit (3) is slidably disposed on the sampling tube (1), and a bottom liquid sampling unit (4) is detachably installed at the lower end of the operating rod (2). The upper end of the sampling tube (1) is detachably fitted with a tube cap (101), and the upper end of the operating rod (2) passes upward through the tube cap (101). The suspension sampling unit (3) includes a first limiting ring (31) and a sampling box (32). The first limiting ring (31) limits the upward movement of the sampling box (32). An elastic retaining ring (3112) is embedded in the inner wall of the first limiting ring (31). The elastic retaining ring (3112) is press-fitted with the sampling tube (1). A hollow buoyancy chamber (33) is formed inside the sampling box (32) by injection molding. A first convex ring (34) is formed on the top of the sampling box (32). The first convex ring (34) is fitted with a nut (35). A sampling chamber (36) is injection molded inside the sampling box (32) and located at the lower part of the buoyancy chamber (33). A sampling hole (361) is provided on the inner wall of the sampling box (32) at a position corresponding to the upper part of the sampling chamber (36). The sampling hole (361) communicates with the sampling chamber (36). An inner tube (37) is provided at the axis of the sampling box (32). A first sealing ring (381) is fitted on the outer wall of the inner tube (37). After the inner tube (37) slides down along the sampling box (32), it closes the sampling hole (361). The upper part of the inner tube (37) is provided with an external threaded part (371), the diameter of which is larger than the inner diameter of the sampling box (32). After the inner tube (37) slides down, the nut (35) engages with the external threaded part (371), and the height of the inner tube (37) is locked at this time. The first sealing ring (381) seals the sampling hole (361). The threaded portion (371) is fitted with a sliding cap (39), which is in transition fit with the sampling tube (1). A second convex ring (121) is provided at the bottom of the outer side of the sampling tube (1). The second convex ring (121) limits the downward movement of the sliding cap (39). The inner diameter of the inner tube (37) is larger than the diameter of the second convex ring (121). After the connection between the external threaded portion (371) and the sliding cap (39) is released, the sampling box (32) is taken out downward along the sampling tube (1). Among them, three or more sliding grooves (3221) are arranged in a ring on the inner wall of the sampling box (32), and an arc-shaped elastic piece (3222) is provided at the lower part of the inner tube (37). The lower end of the elastic piece (3222) is stuck in the sliding groove (3221) and can slide vertically along the sliding groove (3221).

2. The liquid sampling device for itaconic acid fermentation process according to claim 1, characterized in that: A limit ring (21) is installed on the rod body of the operating lever (2). The limit ring (21) limits the upward movement of the operating lever (2). A threaded part (22) is machined on the upper part of the operating lever (2). A rod cap (23) is fitted through the threaded part (22). The rod cap (23) limits the downward movement of the operating lever (2).

3. The liquid sampling device for itaconic acid fermentation process according to claim 2, characterized in that: A groove (24) is machined on the upper part of the operating lever (2), and a scale (2444) is provided in the groove (24).

4. The liquid sampling device for itaconic acid fermentation process according to claim 1, characterized in that: The inner wall of the first convex ring (34) is injection molded to form an annular groove (341), and the outer wall of the nut (35) is injection molded to form a retaining ring (351) that fits into the groove (341). The retaining ring (351) rotates along the groove (341).

5. The liquid sampling device for itaconic acid fermentation process according to claim 1, characterized in that: When the external thread portion (371) engages with the nut (35), the elastic plate (3222) bulges up after being pressed, and the bulging elastic plate (3222) provides support for the bottom liquid sampling unit (4); a convex tube portion (3223) is injection molded at the bottom of the sampling box (32), and under the support of the convex tube portion (3223), the lower end of the bottom liquid sampling unit (4) is lifted off the ground.

6. The liquid sampling device for itaconic acid fermentation process according to claim 5, characterized in that: The bottom liquid sampling unit (4) includes a liquid storage cup (401) and a connecting cap (402). The upper end of the liquid storage cup (401) is machined with an embedded end (4441), which is inserted into the sampling tube (1). The liquid storage cup (401) is provided with a liquid storage chamber (403) inside. The upper end of the liquid storage chamber (403) is machined with an internal thread (441). The bottom of the connecting cap (402) is provided with a connecting part (404). The outside of the connecting part (404) is machined with an external thread (442) that matches the internal thread (441). A liquid inlet hole (405) is machined near the top of the outer wall of the connecting part (404). A sealing gasket (406) is fitted on the outer wall of the connecting part (404). When the connecting part (404) moves along the internal thread (401), the liquid inlet hole (405) is machined near the top of the outer wall of the connecting part (404). When the connecting part (404) moves along the internal thread (401), the liquid inlet hole (405) is machined near the top of the outer wall of the connecting part (404). A sealing gasket (406) is fitted on the outer wall of the connecting part (404). When the thread (441) is screwed down to its limit, the sealing gasket (406) is sandwiched between the liquid storage cup (401) and the connecting cap (402). At this time, the liquid inlet (405) is closed. The top of the connecting cap (402) is machined with a threaded hole (407) that matches the operating rod (2). The first sealing gasket (4442) is clamped on the outer wall of the embedded end (4441). As the operating rod (2) moves upward, the first sealing gasket (4442) seals between the sampling tube (1) and the liquid storage cup (401). The diameter of the liquid storage cup (401) is smaller than the inner diameter of the inner tube body (37). The liquid storage cup (401) is conical, and its diameter gradually decreases downward. After the liquid storage cup (401) is removed, it is positioned by the elastic sheet (3222).

7. The liquid sampling device for itaconic acid fermentation process according to claim 6, characterized in that: A second sealing ring (4222) is fitted on the outer wall of the connecting cap (402), and the second sealing ring (4222) is interference-fitted with the inner wall of the sampling tube (1).

8. The liquid sampling device for itaconic acid fermentation process according to claim 7, characterized in that: An annular slot (4223) is coaxially provided at the bottom of the connecting cap (402). An elastic rubber clamping ring (4224) is inserted into the slot (4223). An annular filter screen (4225) is clamped on the inner side of the clamping ring (4224). The outer wall of the clamping ring (4224) is formed by interference fit with the inner wall of the sampling tube (1). The upper end of the embedded end (4441) is processed with a small diameter portion (4443). The small diameter portion (4443) fits against the inner wall of the filter screen (4225).

Images