Single sperm freezing carrier

Abstract

The invention relates to a single sperm freezing carrier, and relates to the technical field of medical treatment and public health. Comprising a cryopreservation tube, a freezing slide glass and a connecting handle, the freezing slide glass and the connecting handle are integrally formed in a pouring mode, and a plurality of freezing slide glass are stored in one freezing tube. The device has the characteristics of miniaturization, simplification and integration, and increases the number of frozen patients and the number of times of use on the premise of not increasing the freezing charge of the patients.

Description

Technical Field

[0001] The invention relates to the technical field of medical and health care, and in particular to a single sperm freezing carrier. Background Art

[0002] Single sperm cryopreservation technology was first proposed in 1997, and has been explored and improved from the perspectives of cryocarriers, freezing procedures, and cryoprotectant formulations. Among these methods, the advancement of cryocarriers has directly improved single sperm cryopreservation technology.

[0003] There are many non-biological cryocarriers available, such as Cell Sleeper, Cryotop, Cryoleaf, Cryopiece, SpermVD, etc.

[0004] Cell Sleeper is a cryovial-based cell cryopreservation container with an inner tray. Single sperm is added to the droplet (0.5-3.5 μL) on the tray. The tray is placed in a vial and sealed with a screw cap. The sample vial is fumigated 4–5 cm (-115°C–-130°C) above liquid nitrogen vapor for 2 minutes and then stored in liquid nitrogen. The disadvantage is that the tray has a certain height, and the ICSI needle needs to be continuously raised and lowered significantly during single sperm micromanipulation, resulting in a higher risk of needle breakage.

[0005] Cryotop is a mature commercial cryocarrier. It consists of a thin polypropylene strip, a plastic handle and an outer sleeve. It is mainly used for cryopreservation of embryos and oocytes. It was later cited as a method for cryopreservation of micro sperm. A single sperm is transferred to a cryodroplet (1μL) on a thin polypropylene strip of Cryotop, and the Cryotop is immediately placed 4–5cm (-115℃–-130℃) above liquid nitrogen vapor for fumigation for 2 minutes, and then stored in liquid nitrogen. The disadvantage is that Cryotop is long and needs to be micromanipulated in a larger culture dish, which is a serious waste of mineral oil in practice; secondly, the polypropylene strip is extremely thin and located in the center, and cannot fit with the bottom of the dish. During micromanipulation, the polypropylene strip is easy to move, affecting micromanipulation.

[0006] Cryoleaf is mainly composed of transparent polystyrene cryosections, fixed handles and protective sleeves. The cryosections are made by cutting the Falcon culture dish (Falcon 353001, BD Biosciences) into small square slices, and then manually pulling them into small leaves about 22 mm long and 3 mm wide after heating. The cryosections are then fixed on the handle for micromanipulation and cryo-recovery. The disadvantage of this method is that the culture dish is manually pulled, the preparation process is cumbersome, and it is difficult to ensure the degree of crystallization and flatness of the cryosections. The cryosections need to be cut off during recovery. Because the slices are too light, they need to be sunk to the bottom of the dish with a small copper block before micromanipulation.

[0007] Cryopiece is an ultra-thin cryo-slide that can be used to place microdroplets on the cryo-slide and directly placed in a culture dish for micromanipulation. It is more convenient and practical than the previous single sperm cryo-slide. However, the slide is light in weight and is prone to movement when the stage is moved, affecting micromanipulation. In addition, each cryo-tube can only hold one slide, so the storage capacity is small.

[0008] The improved Cryopiece2.0 slide is thicker and more stable, and can be embedded and fixed in the groove of the matching culture dish, and then micromanipulated. Then, the slide is picked up with tweezers, drained of mineral oil, and neatly fumigated and frozen with liquid nitrogen, and then placed in a cryotube for frozen storage. Cryopiece2.0 is improved over 1.0, but a cryotube can only hold one slide, and the storage capacity is small.

[0009] SpermVD is similar to the Cryopiece above. Sperm can be transferred to the 0.8–1μL droplet on SpermVD, and then SpermVD is transferred to a 3.6mL cryotube and immersed in liquid nitrogen for freezing. However, the slide is larger and requires a 3.6mL cryotube. Although 3 droplets can be frozen at a time, they are all on one slide. When thawing, they can only be thawed completely, not in batches, and can only be thawed once for use.

[0010] Therefore, how to provide a miniaturized, simplified, and integrated single sperm cryocarrier has become a technical problem that technicians in this field need to solve urgently. Summary of the invention

[0011] In view of the above situation, the purpose of the present invention is to provide a single sperm cryocarrier, which has the characteristics of miniaturization, simplification and integration, and increases the number of frozen sperm and the number of times of use without increasing the freezing charges for patients.

[0012] To achieve the above-mentioned purpose, the present invention provides a single sperm cryocarrier, comprising: a cryotube, a cryo slide and a connecting handle; the cryo slide and the connecting handle are integrally perfused and formed, and a plurality of the cryo slides are stored in one cryotube.

[0013] Furthermore, the cryopreservation tube is a 2.0 ml cryopreservation tube.

[0014] Furthermore, each cryo-slide is loaded with only one droplet of 0.5-1 μL.

[0015] Furthermore, a clamping portion is provided at one end of the connecting handle.

[0016] Furthermore, the bottom surface of the connecting handle is flat and flush with the cryo-slide.

[0017] Furthermore, the cryopreservation tube is detachably connected to a tube cap.

[0018] The beneficial effects of the present invention are:

[0019] The present invention proposes a single sperm cryocarrier, which has the characteristics of miniaturization, simplicity and integration. Combining the advantages of the above-mentioned cryocarrier, multiple cryocarriers can be stored in one cryopreservation tube, thereby increasing the number of frozen tubes and the number of times of use without increasing the freezing charges for patients. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a state diagram of the pipe cap of the present invention when it is opened;

[0021] Figure 2 is a schematic diagram of the structure of the pipe cap;

[0022] Figure 3 It is a schematic diagram of the structure of the freezing slide of the present invention.

[0023] Among them, 1-cryostorage tube; 2-cryoslide; 3-connecting handle; 4-droplet; 5-clamping part; 6-tube cap. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchanged where appropriate, so that the embodiments of the present application described here. In addition, the terms "including" and "having" and any of their variations are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device comprising a series of steps or units is not necessarily limited to those steps or units clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0026] In the present application, the terms "upper", "lower", "left", "right", "front", "back", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings. These terms are mainly used to better describe the present application and its embodiments, and are not used to limit the indicated devices, elements or components to have a specific orientation, or to be constructed and operated in a specific orientation.

[0027] In addition, some of the above terms may be used to express other meanings in addition to indicating orientation or positional relationship. For example, the term "on" may also be used to express a certain dependency or connection relationship in some cases. For those of ordinary skill in the art, the specific meanings of these terms in this application can be understood according to specific circumstances.

[0028] In addition, the terms "installed", "set", "provided with", "connected", "connected", and "socketed" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral structure; it can be a mechanical connection, or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be an internal connection between two devices, elements, or components. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0029] To achieve the above purpose, Figure 1-3 As shown, the present invention provides a single sperm cryocarrier, comprising: a cryotube 1, a cryomount 2 and a connecting handle 3; the cryomount 2 and the connecting handle 3 are formed by integrated perfusion, and a plurality of the cryomounts 2 are stored in one cryotube 1, preferably 3 to 5 cryomounts 2. The cryomount 2 and the connecting handle 3 are formed by integrated perfusion, and the handle 3 and the cryomount 2 are connected without using an adhesive, thereby reducing the possibility of the connecting handle 3 and the cryomount 2 falling off during freezing. The cryotube 1 is a 2.0ml cryotube 1. The number of frozen tubes and the number of uses are increased without increasing the freezing charges for patients.

[0030] To further optimize the technical solution, the present invention adopts a miniaturized design, each cryo-slide 2 is loaded with only one droplet 4 of 0.5-1 μL, and multiple cryo-slides 2 can be stored in one cryo-tube 1, increasing the number of frozen tubes and the number of times they can be used. The cryo-slide 2 can be made of polystyrene sheets.

[0031] To further optimize the technical solution, a clamping portion 5 is provided at one end of the connecting handle 3 to facilitate subsequent clamping operations.

[0032] To further optimize the technical solution, the bottom surface of the connecting handle 3 is flat and flush with the cryo-slide 2, which can better fit the culture dish and facilitate microscopy. The rear end of the handle is changed from an arc surface to a flat surface, which is convenient for tweezers to grab the cryo-slide 2.

[0033] To further optimize the technical solution, the cryogenic tube 1 is detachably connected to a tube cap 6, thereby facilitating the storage of the cryogenic tube 1.

[0034] The present invention proposes a single sperm cryocarrier, which has the characteristics of miniaturization, simplicity and integration. Combining the advantages of the above-mentioned cryocarrier, multiple cryocarriers can be stored in one cryopreservation tube, thereby increasing the number of frozen tubes and the number of times of use without increasing the freezing charges for patients.

[0035] The above description is only a preferred embodiment of the present invention and does not limit the technical scope of the present invention. Therefore, any slight modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A single sperm cryocarrier, characterized in that: include: Cryostat, cryostat slide and connecting handle; the cryostat slide and connecting handle are integrally formed by perfusion molding, and a plurality of cryostat slides are stored in one cryostat.

2. A single sperm cryocarrier as claimed in claim 1, characterized in that: The cryopreservation tube is a 2.0 ml cryopreservation tube.

3. A single sperm cryocarrier as claimed in claim 1, characterized in that: Each cryo slide was loaded with only one droplet of 0.5-1 μL.

4. A single sperm cryocarrier according to claim 1 or 3, characterized in that: A clamping portion is provided at one end of the connecting handle.

5. A single sperm cryocarrier as claimed in claim 4, characterized in that: The bottom surface of the connecting handle is flat and flush with the cryo-slide.

6. A single sperm cryocarrier as claimed in claim 1, characterized in that: The freezing tube is detachably connected with a tube cap.

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