Preparation and application of composition for regulating intestinal microenvironment

Abstract

The invention discloses preparation and application of a composition for regulating intestinal microenvironment, and belongs to the field of health care products. The composition for regulating the intestinal microenvironment is prepared by compounding raw materials including chickpea extract, peach gum powder, cowpea powder, pomelo peel powder and arrowroot flour. By compounding the raw materials, excellent effects of promoting the growth of beneficial bacteria and improving the intestinal microenvironment are achieved.

Description

Technical Field

[0001] The present invention relates to the field of health care products, and in particular to the preparation and application of a composition for regulating intestinal microenvironment. Background Art

[0002] There is a mutual dependence and mutual influence between the intestinal microenvironment and the intestinal flora, and the two work together to maintain the homeostasis of the intestine. The intestinal flora ensures the integrity of the intestinal barrier function through various mechanisms such as forming biofilm barriers, promoting the proliferation and differentiation of intestinal epithelial cells, and regulating the host's immune system. At the same time, the metabolites of intestinal microorganisms, such as short-chain fatty acids and bile acids, can act on multiple receptors of host cells, thereby regulating the homeostasis of the intestinal microenvironment.

[0003] In addition, the balance of intestinal flora is of vital importance to host health. Dysbiosis is closely related to many diseases, such as obesity, diabetes, inflammatory bowel disease, etc. Therefore, the core purpose of regulating the intestinal microenvironment is to optimize the composition and function of intestinal microorganisms. This regulation not only helps to improve intestinal health, but also has a positive impact on the host's metabolism, immunity and mental state through mechanisms such as the brain-gut axis.

[0004] Based on this, it is necessary to develop a composition that can regulate the intestinal microenvironment and assist in regulating the homeostasis of the intestinal microenvironment by promoting the growth of intestinal probiotics. Summary of the invention

[0005] The purpose of the present invention is to provide a preparation method and application of a composition for regulating the intestinal microenvironment. The composition for regulating the intestinal microenvironment is compounded with chickpea extract, peach gum powder, cowpea powder, grapefruit peel powder and yam powder as raw materials, and has excellent effects of promoting the growth of beneficial bacteria and improving the intestinal microenvironment.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a composition for regulating the intestinal microenvironment, wherein the composition for regulating the intestinal microenvironment comprises the following raw materials in parts by weight:

[0008] Chickpea extract: 10-20 parts;

[0009] Peach gum powder: 10-30 parts;

[0010] Cowpea flour: 10-15 parts;

[0011] Grapefruit peel powder: 20-25 parts;

[0012] Jewthorn powder: 10-30 servings.

[0013] Furthermore, the composition for regulating the intestinal microenvironment comprises the following raw materials in parts by weight:

[0014] Chickpea extract: 15-20 parts;

[0015] Peach gum powder: 20-30 parts;

[0016] Cowpea flour: 10-13 parts;

[0017] Grapefruit peel powder: 23-25 ​​parts;

[0018] Jewthorn powder: 20-30 servings.

[0019] Most preferably, the composition for regulating the intestinal microenvironment comprises the following raw materials in parts by weight:

[0020] Chickpea extract: 15 parts;

[0021] Peach gum powder: 20 parts;

[0022] Cowpea flour: 13 parts;

[0023] Grapefruit peel powder: 23 parts;

[0024] Jewthorn powder: 20 servings.

[0025] In a second aspect, the present invention provides a beverage for regulating the intestinal microenvironment, the beverage comprising the following components in percentage by weight:

[0026] The composition for regulating intestinal microenvironment according to the first aspect: 10%-30%;

[0027] Multivitamins: 0.1%-0.5%;

[0028] Sweetener: 0.01%-0.5%;

[0029] Drinking water to 100%.

[0030] The complex vitamin is one or more of vitamin C, vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, and vitamin B9.

[0031] Most preferably, the vitamin complex consists of vitamin C, vitamin B2, vitamin B3, vitamin B5 and vitamin B9 in a mass ratio of 2:0.1:0.5:1:1.

[0032] The sweetener is one or more of steviol glycoside, xylitol, aspartame, acesulfame potassium, cyclamate, sorbitol and maltitol.

[0033] Most preferably, the sweetener consists of sorbitol and maltitol in a mass ratio of 1:0.3.

[0034] In a third aspect, the present invention provides a method for preparing a beverage for regulating intestinal microenvironment according to the second aspect, the preparation method comprising the following steps:

[0035] The composition for regulating intestinal microenvironment, multivitamins, sweeteners and drinking water are mixed at room temperature, homogenized for 1-2 hours, filled and sterilized.

[0036] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention provides a beverage for regulating intestinal microenvironment, the beverage comprises a composition for regulating intestinal microenvironment, the composition for regulating intestinal microenvironment is compounded by chickpea extract, peach gum powder, cowpea powder, grapefruit peel powder and yam powder, and research has found that there is a synergistic effect among the chickpea extract, peach gum powder, cowpea powder, grapefruit peel powder and yam powder in the present invention, specifically: firstly, the chickpea extract, peach gum powder, cowpea powder, grapefruit peel powder and yam powder are rich in oligosaccharides, dietary fiber and the like, and such substances cannot be digested by human digestive enzymes, but can be utilized by probiotics to promote their growth and metabolism; secondly, the flavonoids and phenolic substances rich in cowpea powder and grapefruit peel powder have significant antioxidant and anti-inflammatory effects, can improve the intestinal oxidative stress state, indirectly promote the growth of probiotics, and enhance the colonization ability of probiotics. DETAILED DESCRIPTION

[0037] In order to better understand the present invention, the present invention is further described below in conjunction with specific application examples, wherein the terms used in the composition are intended to describe specific embodiments and do not constitute limitations on the protection scope of the present invention.

[0038] The test methods without specific conditions in the following tests are usually carried out under conventional conditions or the conditions recommended by the manufacturer. Unless otherwise specified, they are calculated based on mass percentage and mass fraction.

[0039] Some raw materials and their sources are as follows:

[0040] Chickpea extract: purchased from Fufeng Snot Biotechnology Co., Ltd.;

[0041] Peach gum powder: purchased from Henan Jiqian Biotechnology Co., Ltd.;

[0042] Cowpea powder: purchased from Fufeng Snot Biotechnology Co., Ltd.

[0043] Grapefruit peel powder: purchased from Baoji Liupanyun Biotechnology Co., Ltd.

[0044] Jicama powder: purchased from Lanzhou Waterless Biotechnology Co., Ltd.;

[0045] Other raw materials were purchased commercially.

[0046] The raw materials and their weight proportions of the composition for regulating intestinal microenvironment are shown in Table 1.

[0047] Table 1 Raw materials and their mass proportions of the composition for regulating the intestinal microenvironment

[0048]

[0049]

[0050] Probiotic activity test

[0051] Test strains: Bifidobacterium longum (A) was purchased from the Marine Microbiological Culture Collection Administration Center, number: MCCCNO.1K08396, Lactobacillus acidophilus (B) was purchased from the China General Microbiological Culture Collection Administration Center, number: CGMCCNO.1.1878, Lactobacillus rhamnosus (C) was purchased from the China General Microbiological Culture Collection Administration Center, number: CGMCCNO.1.577;

[0052] Experimental culture medium: MRS culture medium (prepared according to the instructions), purchased from Shanghai MacLean Biochemical Technology Co., Ltd., catalog number: M775817;

[0053] Test samples: Compositions 1-3, compositions ①-⑥, each test sample was diluted to 30wt% using MRS medium, sterilized and prepared before use;

[0054] Blank sample: MRS medium;

[0055] The pH of the above test samples and blank samples were adjusted to 6.0 using NaOH and HCl;

[0056] The water used in the test was sterile water.

[0057] Test method:

[0058] 1. Bacteria activation

[0059] The MRS medium prepared according to the MRS medium instructions was sterilized at 121°C for 30 min, and the sterilized MRS medium was dispensed into anaerobic culture tubes in an anaerobic sterile environment for later use; the lyophilized powder of the bacterial strain was dissolved with sterile water in an anaerobic sterile environment, and after being fully mixed, it was allowed to stand until completely precipitated, and the supernatant was inoculated into the anaerobic culture tube medium at a 2% inoculation rate, and cultured at 37°C.

[0060] 2. Effect of the composition on the proliferation of probiotics

[0061] After Bifidobacterium longum, Lactobacillus acidophilus and Lactobacillus rhamnosus were activated and cultured to the stable period, 100 μL of the activated bacterial culture solution in the stable period was added to the anaerobic culture tube containing 9.9 mL of the test sample or blank sample, and incubated at 37°C in an anaerobic environment for 24 hours. After 24 hours, the anaerobic culture tube was taken out and immediately ice-bathed for 5 minutes, and then the pH value of the culture solution in the tube was measured to evaluate the different effects of the test samples on the acid production of the test strains. The pH was measured by a Lei-Magnetic PHSJ-6L pH meter, and the measurement results were retained to two significant digits after the decimal point.

[0062] 3. Effect of the composition on the growth rate of probiotics

[0063] Pour 10mL of test sample or blank sample sterilized at 121℃ for 30min into the anaerobic culture tube. Inoculate the activated strains cultured to the stable period into different anaerobic culture tubes in turn, culture under anaerobic conditions for 48h, and measure the OD600nm of the culture solution in the tube at 1h and 48h to evaluate the growth rate of the three intestinal probiotics.

[0064] Growth rate increment calculation formula: Growth rate increment / % = (A 样2 -A 空2 / A 样1 -A 空1 ) / (A 样1 -A 空1 )*100%

[0065] Where A: OD600 absorbance, A 样2 : OD600 absorbance of the test sample after 48h of culture; A 空2 : OD600 absorbance of blank sample after 48h of culture; A 样1 : OD600 absorbance of the test sample after 1h of culture; A 空1 : OD600 absorbance of blank sample after 1 h of incubation.

[0066] Table 2 Activity test of Bifidobacterium longum, Lactobacillus acidophilus and Lactobacillus rhamnosus

[0067]

[0068]

[0069] Note: In the “pH” column, “#” indicates that compared with composition 1, p < 0.05, “*” indicates that compared with blank, p < 0.05; in the “Growth rate increment” column, “a” indicates that compared with composition 1, p < 0.05.

[0070] Bifidobacterium longum, Lactobacillus acidophilus, and Lactobacillus rhamnosus are all beneficial bacteria in the intestine. By improving their activity and enhancing their growth rate, we can effectively regulate the balance of intestinal flora, improve the intestinal microenvironment, and inhibit the proliferation of harmful bacteria.

[0071] The main secretion products of Bifidobacterium longum, Lactobacillus acidophilus and Lactobacillus rhamnosus in anaerobic environment are lactic acid and acetic acid. The measurement of pH can be used as one of the indicators of the biological activity of these strains.

[0072] According to the results in Table 2, compositions 1-3 of the present invention are significantly different from the blank control, indicating that the chickpea powder, peach gum powder, cowpea powder, grapefruit peel powder and lycopodiella powder described in compositions 1-3 of the present invention have significant effects on promoting the growth of Bifidobacterium longum, Lactobacillus acidophilus and Lactobacillus rhamnosus and promoting the improvement of their biological activity.

[0073] Comparing the results of composition 1 with compositions ①-⑤, it can be seen that the compound use of chickpea extract, peach gum powder, cowpea powder, grapefruit peel powder and yam powder in the present invention has a significant synergistic effect.

[0074] Comparing the results of composition 1 and composition ⑥, it can be seen that the chickpea extract, peach gum powder, cowpea powder, grapefruit peel powder and yam powder in the specific dosage ratio of the present invention have a significant synergistic effect.

[0075] Application Example 1:

[0076] The specific mass percentages of the components in a beverage for regulating intestinal microenvironment are as follows:

[0077] Composition for regulating intestinal microbial environment (composition 1): 20%;

[0078] Multivitamins: 0.3%;

[0079] Sweetener: 0.3%;

[0080] Drinking water replenished to 100%;

[0081] The complex vitamins are composed of vitamin C, vitamin B2, vitamin B3, vitamin B5 and vitamin B9 in a mass ratio of 2:0.1:0.5:1:1; the sweeteners are composed of sorbitol and maltitol in a mass ratio of 1:0.3.

[0082] Application Example 2:

[0083] The specific mass percentages of the components in a beverage for regulating intestinal microenvironment are as follows:

[0084] Composition for regulating intestinal microbial environment (composition 1): 30%;

[0085] Multivitamins: 0.1%;

[0086] Sweetener: 0.5%;

[0087] Drinking water replenished to 100%;

[0088] The complex vitamins are composed of vitamin C, vitamin B1, vitamin B3, vitamin B6 and vitamin B7 in a mass ratio of 2:0.1:0.5:1:1; the sweeteners are composed of sorbitol and xylitol in a mass ratio of 1:0.3.

[0089] Application Example 3:

[0090] The specific mass percentages of the components in a beverage for regulating intestinal microenvironment are as follows:

[0091] Composition for regulating intestinal microbial environment (composition 1): 10%;

[0092] Multivitamins: 0.5%;

[0093] Sweetener: 0.1%;

[0094] Drinking water replenished to 100%;

[0095] The complex vitamins are composed of vitamin C, vitamin B2, vitamin B3, vitamin B5 and vitamin B9 in a mass ratio of 2:0.1:0.5:1:1; the sweeteners are composed of stevioside and aspartame in a mass ratio of 1:0.3.

[0096] The specific steps of the beverage preparation method for regulating the intestinal microenvironment in the above application examples 1-3 are as follows:

[0097] The composition for regulating intestinal microenvironment, multivitamins, sweeteners and drinking water are mixed at room temperature, homogenized for 2 hours, filled and sterilized.

[0098] Sensory evaluation

[0099] According to the detection method and sensory requirements described in GB 7101-2022 "National Food Safety Standard Beverages", 10 professionally trained sensory evaluators were selected to evaluate Application Examples 1-3 of the present invention.

[0100] Evaluation results: The beverages for regulating the intestinal microenvironment prepared in Application Examples 1-3 of the present invention have uniform color, the color that the product should have, a slightly sweet taste, a unique plant fragrance, no peculiar taste, no peculiar smell, a uniform state, and no foreign matter visible to normal vision.

[0101] The embodiments described above are part of the embodiments of the present invention, rather than all of the embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

Claims

1. A composition for regulating intestinal microenvironment, characterized in that: Including the following raw materials by weight: Chickpea extract: 10-20 parts; Peach gum powder: 10-30 parts; Cowpea flour: 10-15 parts; Grapefruit peel powder: 20-25 parts; Jewthorn powder: 10-30 servings.

2. The composition according to claim 1, characterized in that Including the following raw materials by weight: Chickpea extract: 15-20 parts; Peach gum powder: 20-30 parts; Cowpea flour: 10-13 parts; Grapefruit peel powder: 23-25 ​​parts; Jewthorn powder: 20-30 servings.

3. The composition according to claim 1, characterized in that Including the following raw materials by weight: Chickpea extract: 15 parts; Peach gum powder: 20 parts; Cowpea flour: 13 parts; Grapefruit peel powder: 23 parts; Jewthorn powder: 20 servings.

4. A beverage for regulating intestinal microenvironment, characterized in that: A composition for regulating intestinal microenvironment comprising any one of claims 1 to 3.

5. The beverage according to claim 4, characterized in that: Contains the following components by mass percentage: Composition for regulating intestinal microenvironment: 10%-30%; Multivitamins: 0.1%-0.5%; Sweetener: 0.01%-0.5%; Drinking water to 100%.

6. The beverage according to claim 5, characterized in that: The complex vitamin is one or more of vitamin C, vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, and vitamin B9.

7. The beverage according to claim 5, characterized in that: The sweetener is one or more of steviol glycoside, xylitol, aspartame, acesulfame potassium, cyclamate, sorbitol and maltitol.

8. A method for preparing a beverage according to any one of claims 5 to 7, characterized in that: The following steps are involved: The composition for regulating intestinal microenvironment, multivitamins, sweeteners and drinking water are mixed at room temperature, homogenized for 1-2 hours, filled and sterilized.

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