Composition for promoting bone growth and physical development of children and preparation method thereof

Abstract

The invention discloses a composition for promoting skeletal growth and physical development of children and a preparation method, the composition comprises a plurality of nutrient elements and Chinese herbal medicine components, and the components have the effects of promoting skeletal health, enhancing immunity, improving nutrient absorption, regulating metabolism, improving sleep quality, promoting secretion of growth hormone and the like. The healthy growth of bones and the overall development of a body are jointly promoted.

Description

Technical Field

[0001] The invention relates to the technical field of functional foods, in particular to a composition for promoting children's bone growth and body development and a preparation method thereof. Background Art

[0002] Adolescence is a critical stage of human growth and development. Height, as one of the important indicators of adolescent growth and development, is affected by many factors, including genetics, nutrition, and lifestyle. With the development of modern nutrition, people are paying more and more attention to promoting the healthy growth of adolescents through reasonable diet and nutritional supplements. Summary of the invention

[0003] The invention provides a composition, which is used for promoting bone growth and body development of children by rationally matching diet and nutrients.

[0004] In view of this, the scheme of the present invention is:

[0005] The first aspect of the present invention is to provide a composition for promoting bone growth and physical development of children, which is composed of 10-30 parts of oligofructose, 10-30 parts of concentrated whey protein, 20-40 parts of bovine collagen peptide, 5-30 parts of hawthorn, 5-30 parts of malt, 5-30 parts of astragalus, 5-30 parts of red dates, 5-15 parts of oysters, 5-20 parts of spiny jujube kernels, 0.5-10 parts of gamma-aminobutyric acid, 0.25-10 parts of calcium citrate, 0.25-10 parts of calcium gluconate, and 0.5-10 parts of citric acid.

[0006] Furthermore, the composition is composed by weight: 10 parts of oligofructose, 15 parts of concentrated whey protein, 25 parts of bovine collagen peptide, 10 parts of hawthorn, 15 parts of malt, 5 parts of astragalus, 5 parts of red dates, 8 parts of oysters, 10 parts of spiny jujube kernels, 0.5 parts of γ-aminobutyric acid, 0.5 parts of calcium citrate, 0.25 parts of calcium gluconate, and 2 parts of citric acid.

[0007] The second aspect of the present invention is to provide a method for preparing the above-mentioned composition, which comprises the following steps: taking measured amounts of hawthorn, malt, astragalus, red dates, oysters, and spiny jujube seeds for decoction, filtering, concentrating the filtrate, and spray-drying it to obtain fine powder, and uniformly mixing the fine powder with measured amounts of oligofructose, concentrated whey protein, bovine collagen peptide, γ-aminobutyric acid, calcium citrate, calcium gluconate, and citric acid to obtain a product.

[0008] Furthermore, the decoction process comprises the steps of re-decocting the filter residue obtained by filtration and combining the filtrate.

[0009] Furthermore, the material-liquid ratio in the decoction process is 1:(8-15), and the decoction time is 1-3h.

[0010] Furthermore, the mesh size used in the filtering process is 200 meshes.

[0011] Furthermore, before the decoction step, hawthorn, malt, astragalus, red dates, oysters, and spiny jujube seeds are mixed and crushed, soaked in water, and then added with yeast, Lactobacillus plantarum, and Lactobacillus bulgaricus for fermentation.

[0012] Preferably, during the fermentation process, the initial OD of the added yeast is 600 =0.1, and after 12 hours, 3-5% of mixed bacteria of Lactobacillus plantarum and Lactobacillus bulgaricus were inoculated, and the ratio of Lactobacillus plantarum to Lactobacillus bulgaricus was 1:1.

[0013] Preferably, the fermentation process controls the pH value to be 4.0-6.5 and the temperature to be 30-37°C.

[0014] Preferably, the fermentation process is micro-aerobic in the early stage and static anaerobic in the later stage; the fermentation time is 24-48 hours.

[0015] The third aspect of the present invention is to propose the use of the composition described in the first aspect or the composition obtained by the preparation method described in the second aspect in the preparation of products for promoting children's bone growth and body development.

[0016] Furthermore, the product is a food, including at least one of the following non-pharmaceutical uses:

[0017] a) Promote bone growth and development;

[0018] b) Enhance bone strength;

[0019] c) Increase in weight and height;

[0020] d) Improve your diet.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The composition provided by the present invention comprises a variety of nutritional elements and Chinese herbal medicine ingredients. Fructooligosaccharides, as a prebiotic, can promote intestinal health and help improve nutrient absorption. Concentrated whey protein and bovine collagen peptides provide high-quality protein and amino acids to promote the development of bones and muscles. Traditional herbal ingredients such as hawthorn, malt and astragalus can enhance the body's metabolic function and immunity. Red dates and oysters are rich in vitamins and minerals, which help maintain physical health and promote growth and development. Ziziphus jujuba seeds and gamma-aminobutyric acid may help improve sleep quality, and good sleep is a key factor in promoting the secretion of growth hormone. Calcium citrate and calcium gluconate provide easily absorbed calcium to maintain bone health. These ingredients have the effects of promoting bone health, enhancing immunity, improving nutrient absorption and regulating metabolism, improving sleep quality, promoting the secretion of growth hormone, etc., and jointly promote the healthy growth of bones and the overall development of the body. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 These are the results of the effects of the two nutrient solutions on H2O2-induced osteoblast activity in Experimental Example 1.

[0024] Figure 2 These are the results of the effects of the two nutrient solutions on osteoblast differentiation induced by oxidative damage in Experimental Example 1.

[0025] Figure 3 These are the results of the effects of the two nutrient solutions in Experimental Example 1 on H2O2-induced ROS production and oxidase in osteoblasts.

[0026] Figure 4 These are the results of the effects of the two nutrient solutions on the femur length and bone weight of mice in Experimental Example 2.

[0027] Figure 5 These are the results of the effects of the two nutrient solutions on mouse serum IGF-1 and OCN in Experimental Example 2. DETAILED DESCRIPTION

[0028] The technical solution of the present invention will be clearly and completely described below in conjunction with the preferred embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Example 1

[0030] A composition for promoting bone growth and physical development of children, comprising, by weight, 10 parts of oligofructose, 15 parts of concentrated whey protein, 25 parts of bovine collagen peptide, 10 parts of hawthorn, 15 parts of malt, 5 parts of astragalus, 5 parts of red dates, 8 parts of oysters, 10 parts of spiny jujube kernels, 0.5 parts of gamma-aminobutyric acid, 0.5 parts of calcium citrate, 0.25 parts of calcium gluconate and 2 parts of citric acid.

[0031] Example 2

[0032] A composition for promoting bone growth and physical development of children, comprising, by weight, 15 parts of oligofructose, 10 parts of concentrated whey protein, 20 parts of bovine collagen peptide, 8 parts of hawthorn, 8 parts of malt, 10 parts of astragalus, 10 parts of red dates, 5 parts of oysters, 15 parts of spiny jujube kernels, 1 part of gamma-aminobutyric acid, 1 part of calcium citrate, 1 part of calcium gluconate, and 1.5 parts of citric acid.

[0033] Example 3

[0034] A composition for promoting bone growth and physical development of children, comprising, by weight, 25 parts of oligofructose, 30 parts of concentrated whey protein, 40 parts of bovine collagen peptide, 25 parts of hawthorn, 25 parts of malt, 10 parts of astragalus, 15 parts of red dates, 15 parts of oysters, 20 parts of spiny jujube kernels, 1 part of gamma-aminobutyric acid, 1 part of calcium citrate, 0.5 parts of calcium gluconate and 3.5 parts of citric acid.

[0035] Comparative Example 1

[0036] This comparative example is different from Example 1 in that hawthorn, malt and astragalus are not contained, and other components and amounts are the same.

[0037] Comparative Example 2

[0038] This comparative example is different from Example 1 in that red dates and oysters are not contained, and other components and amounts are the same.

[0039] Comparative Example 3

[0040] This comparative example is different from Example 1 in that it does not contain spiny jujube seeds and γ-aminobutyric acid, and other components and amounts are the same.

[0041] Preparation Example 1

[0042] First, take the weighed hawthorn, malt, astragalus, red dates, oysters, and spiny date seeds, add 10 times of purified water, soak for 30 minutes, then heat and boil for 2 hours, filter the decoction with a 200-mesh sieve, and set aside the filtrate, continue to add 8 times of purified water for boiling, keep boiling for 2 hours, filter with a 200-mesh sieve, combine the last filtrate, concentrate and spray-dry to obtain fine powder, and mix the fine powder with the weighed oligofructose, concentrated whey protein, bovine collagen peptide, γ-aminobutyric acid, calcium citrate, calcium gluconate, and citric acid to obtain mixture 1. Add an appropriate amount of purified water to mixture 1 to prepare nutrient solution 1 for use.

[0043] Preparation Example 2

[0044] First, weigh hawthorn, malt, astragalus, red dates, oysters, and spiny jujube kernels, add 10 times purified water, crush into coarse powder, pass through an 8-mesh sieve, add 2 times water to soak, and then add a culture medium containing yeast, plant lactobacillus, and bulgaricus for mixed fermentation. The fermentation conditions are as follows:

[0045] 1) Initial pH: 6.0-6.5. At the same time, calcium carbonate (CaCO3, 2-5 g / L) is added to neutralize lactic acid to prevent pH < 4.0 from inhibiting yeast activity.

[0046] 2) Temperature: 30-37°C, first 12h: micro-aerobic (shaking speed 50-100rpm), later period: static anaerobic.

[0047] 3) Yeast (initial OD 600 =0.1), and 12 hours later, Lactobacillus plantarum + Lactobacillus bulgaricus (1:1 ratio, total inoculation amount 3-5%) were inoculated.

[0048] 4) Sterilization conditions: 121°C, 15-20 min; Fermentation time: 24-48 h; End point determination: pH ≤ 4.0 (lactic acid content ≥ 2.0 g / L), number of live yeast cells ≥ 1 × 10 8 CFU / mL, Lactobacillus ≥1×10 9 CFU / mL -1 .

[0049] After the fermentation is completed, 8 times the amount of water is added, heated and boiled for 2 hours, the decoction is filtered through a 200-mesh sieve, and the filtrate is set aside. 8 times the amount of purified water is continued to be added for boiling for 2 hours, filtered through a 200-mesh sieve, the last filtrate is combined, concentrated and spray-dried to obtain fine powder, and the fine powder is mixed with the weighed oligofructose, concentrated whey protein, bovine collagen peptide, γ-aminobutyric acid, calcium citrate, calcium gluconate, and citric acid to obtain mixture 2. An appropriate amount of purified water is added to mixture 2 to prepare nutrient solution 2 for standby use.

[0050] Preparation Example 1 and Preparation Example 2 were prepared according to the composition formulas of Examples 1-3, respectively. The solid matter (the fine powder after the decoction was concentrated and dried, or the mixture after the fine powder after the fermentation liquid was concentrated and dried was compounded according to the preparation example) was added with an appropriate amount of purified water to prepare nutrient solutions with concentrations of 50 μg / mL and 200 μg / mL, respectively, for standby use.

[0051] Experimental Example 1 Cell Experiment

[0052] 1. Experimental Methods

[0053] 1.1 Cell culture

[0054] The MC3T3-E1 cells used are mouse calvarial osteoblasts purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The culture conditions are α-MEM culture medium containing 0.1 mg / ml streptomycin, 100 U / ml penicillin and 10% fetal bovine serum. The cells were cultured in an incubator at 37°C and 5% CO2 saturated humidity, and passaged with 0.25% trypsin.

[0055] 1.2CCK8 detection of cell viability

[0056] 1000 cells / well were inoculated in a 96-well plate, and a blank control group, a H2O2 group (150μmol / LH2O2 treatment), a low-dose nutrient solution group (H2O2+50μg / mL nutrient solution), and a high-dose nutrient solution group (H2O2+200μg / mL nutrient solution) were set up. After 48h of nutrient solution treatment, H2O2 was added for 6h. Subsequently, 10μL CCK-8 solution was added to each well and incubated for 1h. The absorbance was measured at 450nm.

[0057] 1.2 Alkaline phosphatase (ALP) activity and cellular bone mineralization nodule detection

[0058] MC3T3-E1 cells were cultured in osteogenic induction medium (α-MEM containing 10% FBS, 50 μg / mL ascorbic acid, 10 mmol / L β-phosphoglycerol and 10 nmol / L dexamethasone) for 7 or 21 days for the detection of ALP and calcified nodules. ALP activity was tested according to the ALP kit and quantitative comparative analysis was performed. After fixing the cells with 4% paraformaldehyde, they were stained with 0.2% alizarin red solution and the floating color was removed after 1 hour. Each well was incubated with 100 mM hexadecylpyridinium chloride for 1 hour, and the optical density value of the supernatant containing released soluble alizarin red S was measured at 570 nm.

[0059] 1.3 Changes in reactive oxygen species (ROS) production and antioxidant enzyme activity in osteoblasts

[0060] The fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) assay kit was used to measure the intracellular ROS level. DCFH-DA (final concentration 10 μM) was added to each sample group, incubated in a 37°C cell culture incubator for 20 min, and measured after PBS washing. The microplate reader was used to detect fluorescence using an excitation wavelength of 488 nm and an emission wavelength of 525 nm.

[0061] Cells from each sample group were collected, lysed on ice with 0.1 M HCl and 0.1% Triton X-100, and the cell lysates were collected. The activities of antioxidant enzymes GPx and CAT were measured using biochemical kits (Nanjing Jiancheng Biotechnology Institute).

[0062] 2. Experimental results

[0063] The following are experimental results of preparing two different concentrations of nutrient solution 1 and nutrient solution 2 according to Preparation Examples 1 and 2 in Example 1, respectively, and testing them at concentrations of 200 μg / mL and 50 μg / mL.

[0064] 2.1 Effect of nutrient solution on H2O2-induced osteoblast viability

[0065] The two nutrient solutions of 200 μg / mL and 50 μg / mL were treated for 48 h, and then H2O2 was added for induction for 6 h. Compared with the H2O2 group, the cell viability of the high-dose group of nutrient solution 1, the low-dose group of nutrient solution 2, and the high-dose group increased (P<0.01)( Figure 1 ). This indicates that both the high-dose group of nutrient solution 1 and the low-dose and high-dose groups of nutrient solution 2 can protect osteoblasts from H2O2-induced oxidative damage. At the same concentration, the protective effect of nutrient solution 2 group was stronger than that of nutrient solution 1 group.

[0066] 2.2 Effect of nutrient solution on osteoblast differentiation induced by oxidative damage

[0067] ALP rich in cytoplasm in the early stage of osteoblast differentiation and a large amount of calcium salt crystals released into the extracellular matrix in the late stage of differentiation are specific markers of osteoblast differentiation ability. H2O2 can significantly reduce the expression of ALP positive (blue-purple crystals) and reduce the degree of mineralization. However, pretreatment with nutrient solution 1 high-dose group, nutrient solution 2 low-dose and high-dose groups for 48 hours can restore the downregulation of ALP expression and the reduction of calcium nodules induced by H2O2, with significant differences (P<0.01 or P<0.05) ( Figure 2 ). In short, the high-dose group of nutrient solution 1 and the low-dose and high-dose groups of nutrient solution 2 can inhibit the weakening of osteoblast differentiation ability induced by H2O2.

[0068] 2.3 Effects of nutrient solution on H2O2-induced ROS generation and oxidase in osteoblasts

[0069] The results of the effects of nutrient solution on ROS generation and oxidase induced by oxidative damage are as follows Figure 3 As shown. Compared with the control, H2O2 exposure significantly increased the intracellular ROS level, which was about 3 times that of the blank group. Compared with the model group, the low- and high-dose groups of nutrient solution 1 and nutrient solution 2 could significantly reduce the ROS level (Figure A, P<0.01), promote the activity of GPx and CAT, and have a certain dose-dependency, with the high-dose group of nutrient solution 2 being the most significant (Figure B, C). This shows that the nutrient solution can effectively inhibit the accumulation of intracellular ROS induced by H2O2, increase the activity of the cellular antioxidant enzyme system, and prevent osteoblast dysfunction caused by oxidative damage.

[0070] The above results show that the low-dose group of nutrient solution 1 and the low-dose and high-dose groups of nutrient solution 2 can inhibit oxidative damage, promote osteoblast differentiation, improve osteoblast mineralization function, increase the activity of cellular antioxidant enzyme system, and prevent osteoblast dysfunction caused by oxidative damage. The efficacy of the high-dose group of nutrient solution 2 is relatively more significant.

[0071] Experimental Example 2 Animal Experiment

[0072] 1. Experimental Methods

[0073] 1.1 Animals

[0074] 100 3-4 week old SPF Kunming mice (weight about 12 g, half male and half female, purchased from Hunan Slake Jingda Experimental Animal Co., Ltd.), license number: (SCXK (Xiang) 2019-0004) were used. The animals were kept in a standard animal room and adaptively fed for 1 week.

[0075] 1.2 Animal grouping

[0076] All test subjects were randomly divided into groups, with 10 female mice and 10 male mice in each group. There was no significant difference in the initial weight and height of the mice in each group. The blank control group was given normal saline, and the drug-treated group was given 10ml / kg and 50ml / kg of the low and high doses of nutrient solution 1 and nutrient solution 2 by gavage, respectively (equivalent to 1 and 5 times the clinical dose). The experimental period was 28 days.

[0077] 1.3 Detection indicators

[0078] The male and female mice in each group were housed in separate cages and fed freely. The fur and activity of the mice were observed daily, and the feed consumption of the mice in the control group and each experimental group within 24 hours was weighed regularly. The height, weight and food intake of the mice were measured on the 0th, 7th, 14th, 21st and 28th days of the experiment. The levels of serum insulin-like growth factor-1 (IGF-1) and serum osteocalcin (OCN) were detected by ELISA kit after venous blood sampling. The mice were observed daily, and the vaginal opening time of female mice and the testicular descent time of male mice were recorded. Venous blood was collected on the 0th, 7th, 14th and 28th days of the experiment. After the 28th day of the experiment, all the test mice were killed, the bilateral femurs of the mice were separated, and the thymus, spleen and bilateral adrenal glands were quickly removed and weighed.

[0079] 2. Results

[0080] 2.1 For the aforementioned Examples 1-3 and Comparative Examples 1-3, two different nutrient solutions were prepared and compared at different concentrations. The weight, height and food intake of the mice are shown in Table 1.

[0081] Table 1: Comparison of body weight, height and food intake of mice in the drug-treated groups on day 28 of the experiment

[0082]

[0083] At the beginning of the experiment, there was no significant difference in the weight and length of mice in each group, and the difference gradually increased with the passage of feeding time. From the 14th day of the experiment, there were certain differences in weight and length between the groups; on the 28th day, the low-dose and high-dose nutrient solution groups had significant effects on the weight, length and food intake of male and female mice (P<0.01 or P<0.05), and had a certain dose dependence, and the high-dose nutrient solution 2 group was relatively more significant.

[0084] In addition, from the results of Comparative Examples 1-3, it can be seen that when Comparative Example 1 does not contain hawthorn, malt and astragalus, the drug effect is relatively weakened compared with Example 1. However, compared with the control group, Comparative Example 1 still has a significant effect on the body weight and height of male and female mice (P<0.05).

[0085] Comparative Example 2 does not contain red dates and oysters. Compared with the control group, Comparative Example 2 has a significant effect on the body weight, body length and food intake of male and female mice (P<0.05). However, compared with Implementation 1 and Comparative Example 1, the growth of body length is weakened.

[0086] Comparative Example 3 does not contain spina jujuba seeds and γ-aminobutyric acid. Compared with the control group, Comparative Example 3 increased the food intake and body weight of male and female mice (P<0.05), but had no significant effect on body length (P>0.05).

[0087] The effects of the above ingredients on promoting the weight, height and food intake of mice are shown to be a synergistic effect of several ingredients.

[0088] 2.2 Femoral length and bone weight of mice

[0089] like Figure 4 As shown, compared with the blank control group, the mice intervened by the low-dose and high-dose groups of nutrient solution 1 and nutrient solution 2 (both prepared by Example 1) had increased femur length and bone weight, and had a certain dose dependence (P<0.01 or P<0.05). This shows that the nutrient solution can promote bone growth and development and enhance bone strength, and the high-dose group of nutrient solution 2 is relatively more significant. The comparison results of the high-dose group of nutrient solution 2 prepared by Examples 1-3 and Comparative Examples 1-3 are shown in Table 2:

[0090] Table 2:

[0091]

[0092] From the results of Comparative Examples 1-3, it can be seen that compared with Example 1, when Comparative Example 1 does not contain hawthorn, malt and astragalus, the femur length and bone weight still have a significant increase trend compared with the control group (P<0.01 or P<0.05), but the efficacy is weakened compared with Example 1.

[0093] Comparative Example 2 does not contain red dates and oysters. The femur length and bone weight still have a significant increase trend compared with the control group (P<0.05), but the efficacy is weakened compared with Example 1.

[0094] Comparative Example 3 did not contain spina jujuba seeds and γ-aminobutyric acid. The femur length and bone weight increased slightly compared with the control group, but no significant difference was found (P>0.05).

[0095] This indicates that the above ingredients have a synergistic effect in promoting femur length and bone weight.

[0096] The following are experimental results of preparing two different concentrations of nutrient solution 1 and nutrient solution 2 according to Preparation Examples 1 and 2 in Example 1, respectively, and testing them at concentrations of 200 μg / mL and 50 μg / mL.

[0097] 2.3 Changes in IGF-1 and OCN Content in Mice

[0098] IGF-1 is the main hormone required for growth and an important marker for evaluating bone maturity; OCN is a substance specifically secreted by osteoblasts and is one of the markers of bone formation. Figure 5As shown in the figure, compared with the blank control group, on the 21st day, the high-dose nutrient solution 2 group promoted the secretion of OCN (P<0.05). On the 28th day, the mice intervened by nutrient solution 1 and nutrient solution 2 could promote the secretion of IGF-1 and OCN (P<0.01 or P<0.05), and had a certain dose-dependency, and the high-dose nutrient solution 2 group was relatively more significant.

[0099] 2.4 Sexual maturity of mice

[0100] The testicles of male rats in the blank control and nutrient solution groups were all decreased on the 21st day of the experiment, with no significant difference between the groups; there was also no significant difference between the groups in the vaginal opening time of female rats (P>0.05).

[0101] 2.5 Weights of major glands and organs of mice

[0102] As shown in Table 3, the adrenal organ coefficients of the high-dose group of nutrient solution 1 and the low-dose and high-dose groups of nutrient solution 2 were relatively higher than those of the control group (P<0.05), and the thymus organ coefficient of the high-dose group of nutrient solution 2 was relatively higher than that of the control group, with statistically significant differences (P<0.05).

[0103] Table 3:

[0104]

[0105]

[0106] 3. Summary

[0107] Both nutrient solution 1 and nutrient solution 2 can adjust gastrointestinal motility, promote appetite, increase the weight of normal mouse adrenal glands, enhance the body's immune function, promote the secretion of growth hormones such as IGF-1 and OCN, and promote the growth of mouse weight and height. No side effect of promoting early puberty in mice was found. It is expected to be further developed into functional food for children. The efficacy of nutrient solution 2 is relatively stronger than that of nutrient solution 1.

[0108] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A composition for promoting bone growth and physical development in children, characterized in that: The composition by weight is: 10-30 parts of oligofructose, 10-30 parts of concentrated whey protein, 20-40 parts of bovine collagen peptide, 5-30 parts of hawthorn, 5-30 parts of malt, 5-30 parts of astragalus, 5-30 parts of red dates, 5-15 parts of oysters, 5-20 parts of spiny jujube seeds, 0.5-10 parts of gamma-aminobutyric acid, 0.25-10 parts of calcium citrate, 0.25-10 parts of calcium gluconate, and 0.5-10 parts of citric acid.

2. The composition according to claim 1, characterized in that The composition comprises, by weight, 10 parts of oligofructose, 15 parts of concentrated whey protein, 25 parts of bovine collagen peptide, 10 parts of hawthorn, 15 parts of malt, 5 parts of astragalus, 5 parts of red dates, 8 parts of oysters, 10 parts of spiny jujube kernels, 0.5 parts of gamma-aminobutyric acid, 0.5 parts of calcium citrate, 0.25 parts of calcium gluconate and 2 parts of citric acid.

3. The method for preparing the composition according to claim 1, characterized in that: The steps include: taking measured amounts of hawthorn, malt, astragalus, red dates, oysters and spiny jujube seeds for decoction, filtering, concentrating the filtrate and spray-drying it to obtain fine powder, and evenly mixing the fine powder with measured amounts of oligofructose, concentrated whey protein, bovine collagen peptide, gamma-aminobutyric acid, calcium citrate, calcium gluconate and citric acid to obtain the obtained powder.

4. The preparation method according to claim 3, characterized in that: The decoction process comprises the steps of re-decoction of the filter residue obtained by filtration and combining the filtrate.

5. The preparation method according to claim 3, characterized in that: The material-liquid ratio in the decoction process is 1:(8-15), and the decoction time is 1-3 hours.

6. The preparation method according to claim 3, characterized in that: The sieve aperture used in the filtration process is 200 meshes.

7. The preparation method according to claim 3, characterized in that: Before the decoction step, hawthorn, malt, astragalus, red dates, oysters and spiny jujube seeds are mixed and crushed, soaked in water, and then added with yeast, plant lactobacillus and bulgaricus for fermentation.

8. The preparation method according to claim 7, characterized in that: During the fermentation process, the initial OD of the added yeast was 600 =0.1, and after 12 hours, 3-5% of mixed bacteria of Lactobacillus plantarum and Lactobacillus bulgaricus were inoculated, and the ratio of Lactobacillus plantarum to Lactobacillus bulgaricus was 1:

1.

9. The preparation method according to claim 8, characterized in that: The fermentation process controls the pH value to be 4.0-6.5 and the temperature to be 30-37°C; And / or, the fermentation process is micro-aerobic in the early stage and static anaerobic in the later stage; the fermentation time is 24-48 hours.

10. Use of the composition according to claim 1 or 2 or the composition obtained by the preparation method according to any one of claims 3 to 9 in the preparation of products for promoting bone growth and body development in children.

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