Traditional Chinese medicine formula for simultaneously treating goose paramyxovirus disease and hemorrhagic enteritis as well as preparation method and application of traditional Chinese medicine formula

Abstract

The invention provides a traditional Chinese medicine preparation for simultaneously treating goose paramyxovirus disease and hemorrhagic enteritis as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The invention provides a traditional Chinese medicine formula for simultaneously treating goose paramyxovirus disease and hemorrhagic enteritis. The traditional Chinese medicine formula comprises the following raw materials in parts by weight: 2-3 parts of carbonized sanguisorba root, 1-2 parts of coptis chinensis, 2-3 parts of golden cypress, 2-3 parts of scutellaria baicalensis, 4-5 parts of folium isatidis and 4-5 parts of hairyvein agrimony. The traditional Chinese medicine composition provided by the invention has a very good treatment effect on both goose paramyxosis and hemorrhagic enteritis, disease sources can be comprehensively controlled, the purposes of preventing and controlling diseases are achieved, meanwhile, the probability that geese generate drug resistance is greatly reduced, optimization of nutrition supply of the geese is facilitated, and the disease resistance of the geese is improved.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine formula for treating goose paramyxovirus disease and hemorrhagic enteritis, and a preparation method and application thereof. Background Art

[0002] Goose paramyxovirus disease is caused by infection with goose paramyxovirus of the Paramyxoviridae family and the Paramyxovirus genus. It is an acute infectious disease of geese characterized by pathological changes in the digestive tract. Sick geese and carriers after recovery are the source of infection, and it is often transmitted horizontally through the digestive tract and respiratory tract. There is no seasonal pattern in the onset of the disease, and it can occur all year round, often showing endemic epidemics. Geese of all breeds are susceptible, with the youngest age of onset being 3 days old and the maximum age being 300 days old. The younger the age, the higher the morbidity and mortality rate, which is more harmful to the goose flock, often causing large-scale deaths, and the mortality rate of goslings can reach more than 90%. Goose hemorrhagic enteritis is an infectious disease caused by pathogenic Escherichia coli. Geese often show depression, diarrhea, occasionally yellow and bloody stools, decreased egg production, and bran-like ulcers in the intestinal mucosa can be seen in autopsy. The morbidity rate can reach 5% to 60%, and the mortality rate can reach 10% to 50%. The above two diseases have a great impact on the goose farming industry and are likely to cause serious economic losses to the goose farming industry.

[0003] At present, in order to reduce the occurrence of the above two diseases in actual production, prevention is mostly carried out by timely inoculation of goose paramyxovirus oil emulsion inactivated vaccine. Broad-spectrum antibiotics such as amoxicillin, chloramphenicol, penicillin, cephalosporin have certain therapeutic effects on goose paramyxovirus disease and goose hemorrhagic enteritis, but long-term use will produce high drug resistance, and serious drug residues will pose a huge threat to human health. In addition, goose paramyxovirus disease and goose hemorrhagic enteritis can also be treated with some traditional Chinese medicine preparations, such as Xiao Muxia-Jiang'an Swordsman and Xiao Muxia-Huchuan Swordsman. For example, Wang Lihua made traditional Chinese medicine preparations with Codonopsis pilosula, Atractylodes macrocephala, Poria cocos, Gardenia jasminoides, Scutellaria baicalensis, Coptis chinensis, Isatis indigotica, Chuanmutong, Plantago, and Talc to treat goose paramyxovirus disease. After 5 days of continuous use, the death rate dropped to 2-3 / d, and the geese basically recovered (Wang Lihua. The occurrence and comprehensive diagnosis and treatment of goose paramyxovirus disease [J]. Animal Husbandry and Veterinary Science and Technology Information, 2020, (06): 170.). However, the existing traditional Chinese medicine treatment methods are slow to take effect, and there is still a high incidence and mortality rate after taking them. Therefore, there is an urgent need for a pharmaceutical composition that is fast-acting, has little toxicity and side effects, and can effectively treat goose paramyxovirus disease and goose hemorrhagic enteritis. Summary of the invention

[0004] The object of the present invention is to provide a Chinese medicine preparation for treating goose paramyxovirus disease and hemorrhagic enteritis and a preparation method thereof. The Chinese medicine prescription of the present invention has the effects of antibacterial and anti-inflammatory, drying dampness and stopping dysentery, detoxification and sore astringence, etc., has a good therapeutic effect on goose paramyxovirus disease and hemorrhagic enteritis, can comprehensively control the source of the disease, achieve the purpose of disease prevention and control, and at the same time greatly reduce the probability of geese developing drug resistance and improve the disease resistance of geese.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides a traditional Chinese medicine formula for treating goose paramyxovirus disease and hemorrhagic enteritis. The formula comprises the following raw materials in parts by weight: 2-3 parts of sanguisorba officinalis charcoal, 1-2 parts of coptis chinensis, 2-3 parts of phellodendron amurense, 2-3 parts of scutellaria baicalensis, 4-5 parts of isatis indigotica and 4-5 parts of agrimony.

[0007] The present invention also provides a method for treating goose paramyxovirus disease and hemorrhagic enteritis with a traditional Chinese medicine preparation, comprising the following treatment methods:

[0008] Method 1: Take radix sanguisorbae, coptis root, phellodendron, scutellaria baicalensis, isatis indigotica, and agrimony as raw medicinal materials for later use;

[0009] Method 2: Wash, drain, and sun-dry the charcoal of Sanguisorba officinalis, Coptis chinensis, Phellodendron amurense, Scutellaria baicalensis, Folium Isatidis, and Agrimoniae scabra, then crush and sieve the sieved materials to obtain the Chinese medicine powder;

[0010] Method 3: Mix the charcoal of Sanguisorba officinalis, Coptis chinensis, Phellodendron amurense, Scutellaria baicalensis, Folium Isatidis, and Herba Agrimoniae, boil with water, and concentrate the decoction to obtain a concentrated Chinese medicine solution.

[0011] Preferably, the moisture content of each medicinal material after sun drying in the method 2 is 12% to 14%; further preferably, the mesh number of the sieve in the method 2 is 300 to 350 meshes.

[0012] Preferably, the decocting step in method 3 is:

[0013] (1) adding water to the mixed medicinal materials, with the mass ratio of medicinal materials to water being 1:10-12, decocting at 90° C.-100° C. for 50-60 min, filtering through 300-500 meshes, and obtaining a first Chinese medicine liquid and a first Chinese medicine residue;

[0014] (2) adding water to the first Chinese medicine residue, with the ratio of the residue to water being 1:7-9, decocting at 90°C-100°C for 35-40 minutes, filtering through 300-500 meshes, and obtaining the second Chinese medicine liquid and the second Chinese medicine residue;

[0015] (3) adding water to the second Chinese medicine residue, with the ratio of residue to water being 1:4-6, decocting at 90°C-100°C for 20-30 minutes, filtering through 300-500 meshes, and obtaining the third Chinese medicine solution;

[0016] Take three times of Chinese medicine liquid and mix them evenly to obtain decoction liquid;

[0017] Preferably, the concentration temperature is 50-70° C.; the concentration time is 1-4 hours; and the mass ratio of the concentrated liquid to the decoction liquid is 1:5-10.

[0018] Preferably, in method 3, auxiliary materials are added to the concentrated Chinese medicine to prepare granules; the auxiliary materials include dextrin and / or starch; the mass ratio of dextrin to the concentrated Chinese medicine is 1:1-4, and the mass ratio of starch to the concentrated Chinese medicine is 1:1-4.

[0019] Preferably, the types of Chinese medicine preparations prepared by methods 1-3 are raw medicinal materials, Chinese medicine powders, Chinese medicine concentrates and granules.

[0020] Preferably, the method of taking the raw medicinal materials of method 1 is to add 2-3 parts of Sanguisorba officinalis charcoal, 1-2 parts of Coptis chinensis, 2-3 parts of Phellodendron chinense, 2-3 parts of Scutellaria baicalensis, 4-5 parts of Isatis indigotica, and 4-5 parts of Agrimoniae to every 100 parts by weight of feed, mix well, and then crush them together with the feed; the crushed particle size is 6-10 mesh.

[0021] Preferably, the method of taking the traditional Chinese medicine powder is: adding 2-3 parts of Sanguisorba officinalis charcoal, 1-2 parts of Coptis chinensis, 2-3 parts of Phellodendron chinense, 2-3 parts of Scutellaria baicalensis, 4-5 parts of Isatis indigotica, and 4-5 parts of Agrimoniae to the feed per 100 parts by weight of the feed.

[0022] Preferably, the method for taking the Chinese medicine concentrate is: dilute every 5g of the concentrate with 10-20kg of water and then administer it orally; when administering orally, for adult geese weighing more than 3.5kg, each goose is administered 20-30mL of the diluted Chinese medicine concentrate per day; for growing geese weighing 2.5-3kg, each goose is administered 15-20mL of the diluted Chinese medicine concentrate per day.

[0023] Preferably, the method for taking the granules is: adding 500 g of granules to every 500-1000 catties of feed for feeding, or adding 500 g of granules to every 500-1000 catties of water for drinking as drinking water.

[0024] The present invention also provides an application of the Chinese medicine formula or the Chinese medicine preparation obtained by the above processing method in preparing medicines for treating goose paramyxovirus disease and hemorrhagic enteritis.

[0025] The principles of the functions of the drugs in the Chinese medicine formula provided by the present invention are as follows:

[0026] Sanguisorba officinalis charcoal: It is shaped like Sanguisorba officinalis slices, with a burnt black surface and brown inside. It has a burnt aroma and a slightly bitter taste. It has astringent and hemostatic effects and can be used to treat various bleeding symptoms. It can effectively treat goose hemorrhagic enteritis; it can also enhance the resistance of geese and prevent the occurrence of infectious respiratory diseases.

[0027] Coptis chinensis: It is the dried rhizome of three plants of the Ranunculaceae family, namely, Coptis chinensis, Coptis chinensis triangularis or Coptis chinensis. It tastes bitter and is cold in nature. It can treat symptoms such as heat toxins, typhoid fever, bacillary dysentery, hot diarrhea and abdominal pain.

[0028] Phellodendron chinense: It is the dried bark of the Rutaceae plant Phellodendron chinense, which is in the form of plates or shallow grooves, bitter in taste and cold in nature. It can clear away heat and purge fire, and has the function of treating symptoms of heat dysentery and bloody stool.

[0029] Scutellaria baicalensis: The main active ingredients of Scutellaria baicalensis are flavonoids, which have biological effects such as antioxidant, anti-inflammatory, and anti-tumor. It can treat lung heat cough caused by goose paramyxovirus.

[0030] Isatis indigotica: It is the leaf of the isatis indigotica plant of the cruciferous family. It tastes bitter and is cold in nature. It can treat symptoms such as sore throat, fever, headache, and mouth ulcers caused by goose paramyxovirus.

[0031] Agrimony: It is the dried aerial part of the plant Agrimony of the Rosaceae family. It is bitter, astringent and flat. Agrimony has the effects of astringent hemostasis, antimalarial, antidysentery, detoxification and tonification. It can treat goose hemorrhagic enteritis.

[0032] The invention provides a Chinese medicine preparation for treating goose paramyxovirus disease and hemorrhagic enteritis, and a preparation method and application thereof. Goose paramyxovirus disease is an acute infectious disease characterized by pathological changes in the digestive tract of geese. The intestinal mucosa of sick geese has bleeding to varying degrees, and yellow-white thin feces or watery feces are pulled, sometimes with blood and dark red. After drinking water, sick geese have phenomena such as head shaking, coughing, and breathing difficulties. Hemorrhagic enteritis is diarrhea in geese, and thin feces with black or bright red blood are discharged, and intestinal mucosa is seen to fall off. Poor spirit, loss of appetite or hunger. In treatment, antibacterial and anti-inflammatory, dampness-drying and dysentery-stopping, and hemostasis should be the main treatments. According to the compatibility principle of Chinese medicine prescriptions, the prescription is formulated, and radix sanguisorbae, coptis root, phellodendron, scutellaria baicalensis, isatis indigotica, and agrimony are combined, and goose paramyxovirus disease and hemorrhagic enteritis are treated by direct administration or by mixing the Chinese medicine components with feed. By using the Chinese medicine formula provided by the present invention, the pathogens can be controlled in all aspects to achieve the purpose of disease prevention and control, and at the same time, the probability of geese developing drug resistance is greatly reduced, which is beneficial to the optimization of geese's nutritional supply and the improvement of geese's disease resistance. DETAILED DESCRIPTION

[0033] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0034] Example 1

[0035] The invention provides a traditional Chinese medicine preparation (raw medicinal material) for treating goose paramyxovirus disease and hemorrhagic enteritis. The traditional Chinese medicine preparation is prepared from the following medicinal materials in parts by weight: 2 parts of sanguisorba officinalis charcoal, 1 part of coptis chinensis, 2 parts of phellodendron amurense, 2 parts of scutellaria baicalensis, 4 parts of isatis indigotica and 4 parts of agrimony.

[0036] The specific preparation process is as follows:

[0037] Take the raw materials of Sanguisorba officinalis charcoal, Coptis chinensis, Phellodendron chinense, Scutellaria baicalensis, Folium Isatidis, and Herba Agrimoniae, and mix them directly into the feed: add 0.2 g of Sanguisorba officinalis charcoal, 0.1 g of Coptis chinensis, 0.2 g of Phellodendron chinense, 0.2 g of Scutellaria baicalensis, 0.4 g of Folium Isatidis, and 0.4 g of Herba Agrimoniae to every 100 g of feed; stir well and grind them together with the feed, and pass through an 8-mesh sieve; take the crushed medicinal materials and feed and feed them.

[0038] Example 2

[0039] The invention provides a traditional Chinese medicine preparation (traditional Chinese medicine powder) for treating goose paramyxovirus disease and hemorrhagic enteritis. The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 2 parts of sanguisorba officinalis charcoal, 1 part of coptis root, 2 parts of phellodendron amurense, 2 parts of scutellaria baicalensis, 4 parts of isatis indigotica and 4 parts of agrimony.

[0040] The specific preparation process is as follows:

[0041] Take the raw materials of Sanguisorba officinalis charcoal, Coptis chinensis, Phellodendron amurense, Scutellaria baicalensis, Isatis indigotica folia and Agrimoniae scabra, wash and drain them, dry them in the sun until the water content is 12%, grind them respectively through a 300-mesh sieve and take the sieved materials to obtain Chinese medicine powder; stir the above-obtained Chinese medicine powder evenly in a blender; take the mixed Chinese medicine powder and add it to the feed, add 0.2 grams of Sanguisorba officinalis charcoal, 0.1 grams of Coptis chinensis, 0.2 grams of Phellodendron amurense, 0.2 grams of Scutellaria baicalensis, 0.4 grams of Isatis indigotica folia and 0.4 grams of Agrimoniae scabra per 100 grams of feed, stir evenly and feed.

[0042] Example 3

[0043] The invention provides a traditional Chinese medicine preparation (granule) for treating goose paramyxovirus disease and hemorrhagic enteritis. The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 2 parts of sanguisorba officinalis charcoal, 1 part of coptis root, 2 parts of phellodendron amurense, 2 parts of scutellaria baicalensis, 4 parts of isatis indigotica and 4 parts of agrimony.

[0044] The specific preparation process is as follows:

[0045] Take the raw materials of Radix Sanguisorbae, Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae, Folium Isatidis, and Herba Agrimoniae, add 10 times the weight of water to the raw materials, boil at 95°C for 60 minutes, filter at 300 mesh, and separate the Chinese medicine liquid from the residue; continue to add 8 times the weight of water to the residue, boil at 95°C for 40 minutes, filter at 300 mesh, and separate the Chinese medicine liquid from the residue; then add 6 times the weight of water to the residue, boil at 95°C for 30 minutes, filter at 300 mesh, and separate the residue from the Chinese medicine liquid; the three times of Chinese medicine liquid The mixture was mixed evenly to obtain a decoction; the decoction was then concentrated by heating at 60°C for 3h, and the mass ratio of the obtained Chinese medicine concentrate to the decoction was 1:5; dextrin was added to the concentrate, and the mass ratio of the concentrate to dextrin was 1:3. A granulator was used to form granules, and granules with uniform particles were screened and sealed in bags for storage, with 500g per bag; when used, 500g of Chinese medicine granules were mixed into 500kg of feed, stirred evenly for feeding, or 500g of Chinese medicine granules were mixed into 1000kg of water for free drinking.

[0046] Test example

[0047] (1) A goose farm has 2,000 breeding geese, of which 50 geese show symptoms of goose paramyxovirus disease and hemorrhagic enteritis. After isolating the diseased breeding geese from the healthy breeding geese, feed is prepared according to the Chinese medicine ratio of Example 1, and the breeding geese are allowed to eat freely for 4 consecutive days. Two weeks later, the disease-free breeding geese isolated did not show symptoms of goose paramyxovirus disease and hemorrhagic enteritis; among the diseased breeding geese, except for 8 breeding geese that were seriously ill and could not eat feed and died, the remaining 42 breeding geese were treated according to the above-mentioned method of feeding feed with added Chinese medicine, and all were cured. If the Chinese medicine of the present invention is not taken, the mortality rate of goslings can reach more than 50%. It shows that taking the Chinese medicine composition of the present application has produced a significant therapeutic effect.

[0048] (2) A goose farm has 5,000 gooses. The feed is prepared according to the method of Example 2, and the adult geese are allowed to eat freely for 4 days. Goslings are also transferred to the goose farm, and the Chinese medicine described in Example 1 is added to the feed, and the goslings are allowed to eat freely for 4 days. After two weeks, the goslings and adult geese are all normal, and no symptoms such as goose paramyxovirus disease and hemorrhagic enteritis have ever appeared. If the Chinese medicine of the present invention is not taken, the mortality rate of the goslings can reach more than 50%.

[0049] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A Chinese medicine formula for treating goose paramyxovirus disease and hemorrhagic enteritis, characterized in that: The invention comprises the following raw materials in parts by weight: 2-3 parts of Sanguisorba officinalis charcoal, 1-2 parts of Coptis chinensis, 2-3 parts of Phellodendron amurense, 2-3 parts of Scutellaria baicalensis, 4-5 parts of Isatis indigotica and 4-5 parts of Agrimoniae scabra.

2. A method for treating goose paramyxovirus disease and hemorrhagic enteritis with a Chinese medicine prescription according to claim 1, characterized in that: The following processing methods are included: Method 1: Take radix sanguisorbae, coptis root, phellodendron, scutellaria baicalensis, isatis indigotica, and agrimony as raw medicinal materials for later use; Method 2: Wash, drain, and sun-dry the charcoal of Sanguisorba officinalis, Coptis chinensis, Phellodendron amurense, Scutellaria baicalensis, Folium Isatidis, and Agrimoniae scabra, then crush and sieve the sieved materials to obtain the Chinese medicine powder; Method 3: Mix the charcoal of Sanguisorba officinalis, Coptis chinensis, Phellodendron amurense, Scutellaria baicalensis, Folium Isatidis, and Herba Agrimoniae, add water and boil, filter, and concentrate the decoction to obtain a Chinese medicine concentrate.

3. The processing method according to claim 2, characterized in that: The water content of each medicinal material after sun drying in method 2 is 12% to 14%.

4. The processing method according to claim 2, characterized in that: The concentration temperature in method 3 is 50-70° C.; the concentration time is 1-4 hours; and the mass ratio of the concentrated liquid to the decoction liquid is 1:5-10.

5. The processing method according to claim 4, characterized in that: The Chinese medicine concentrate obtained by method 3 is added with auxiliary materials to prepare granules; the auxiliary materials include dextrin and / or starch; the mass ratio of dextrin to the Chinese medicine concentrate is 1:1-4, and the mass ratio of starch to the Chinese medicine concentrate is 1:1-4.

6. The Chinese medicine preparation obtained by the treatment method according to any one of claims 2 to 5, characterized in that: Including raw medicinal materials, Chinese medicine powder, Chinese medicine concentrate and granules.

7. The Chinese medicine preparation according to claim 6, characterized in that: The raw medicinal materials of method 1 are taken directly, and 2-3 parts of Sanguisorba officinalis carbon, 1-2 parts of Coptis chinensis, 2-3 parts of Phellodendron amurense, 2-3 parts of Scutellaria baicalensis, 4-5 parts of Isatis indigotica, and 4-5 parts of Agrimoniae are added to every 100 parts by weight of feed, and the mixture is evenly mixed and then crushed together with the feed; the crushed particle size is 6-10 meshes; The Chinese medicine powder is mixed with feed for consumption, and is added in an amount of 2-3 parts of Sanguisorba officinalis charcoal, 1-2 parts of Coptis chinensis, 2-3 parts of Phellodendron amurense, 2-3 parts of Scutellaria baicalensis, 4-5 parts of Isatis indigotica, and 4-5 parts of Agrimoniae scabra per 100 parts by weight of feed; The concentrated Chinese medicine solution is administered orally. The concentrated solution is diluted before administration, and 10 to 20 kilograms of water are used to dilute every 5 grams of concentrated solution. When administered orally, adult geese weighing more than 3.5 kilograms are administered orally with 20 to 30 milliliters of the diluted concentrated Chinese medicine solution per day for each goose; and 15 to 20 milliliters of the diluted concentrated Chinese medicine solution per day for each goose weighing 2.5 to 3 kilograms for each goose. The granules are mixed into feed for feeding or dissolved in water and used as drinking water. When mixed into feed for feeding, 500 g of granules are added to every 500 to 1000 jin of feed; when used as drinking water, 500 g of granules are added to every 500 to 1000 jin of water.

8. Use of the Chinese medicine formula according to claim 1 or the Chinese medicine preparation according to claim 6 or 7 in preparing medicine for treating goose paramyxovirus disease and hemorrhagic enteritis.