Anti-stress oral liquid as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of traditional Chinese veterinary medicine preparations, and particularly relates to an anti-stress oral liquid as well as a preparation method and application thereof. The anti-stress oral liquid is prepared from the following raw material medicines in parts by weight: 138 to 142 parts of pericarpium citri reticulatae, 38 to 42 parts of fructus ziziphi jujubae, 18 to 22 parts of medicated leaven, 18 to 22 parts of fructus forsythiae, 18 to 22 parts of bupleurum chinense, 18 to 22 parts of radix isatidis, 18 to 22 parts of folium isatidis, 18 to 22 parts of radix astragali, 18 to 22 parts of radix scutellariae, 18 to 22 parts of radix codonopsis, 38 to 42 parts of fructus lycii, 18 to 22 parts of rhizoma atractylodis macrocephalae, 38 to 42 parts of rhizoma dioscoreae, 18 to 22 parts of radix angelicae sinensis and 18 to 22 parts of poria cocos. The pure natural traditional Chinese medicine anti-stress oral liquid is taken as an entry point, and the anti-stress oral liquid which can be freely orally taken by livestock and poultry and can enhance the anti-stress capability by improving the organism immunity of the livestock and poultry is developed. Besides, the anti-stress oral liquid is suitable for various animals, subsequent diseases caused by stress of livestock and poultry can be practically solved, the labor cost is reduced, and the development of animal husbandry is promoted.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese veterinary medicine preparations, and specifically relates to an anti-stress oral liquid and a preparation method and application thereof. Background Art

[0002] Stress refers to the non-specific systemic reaction of the body when it is stimulated by various internal and external environmental factors, which has a serious impact on the feed intake and production performance of animals. It is mainly divided into cold stress, heat stress, transportation stress and immune stress. Among them, cold stress can lead to slower growth rate, decreased production performance, increased disease infection rate and increased mortality in ruminants. For heat stress, it is mainly manifested as a decrease in feed intake. When the ambient temperature is about 30°C and the relative humidity is less than 80%, the feed intake of beef cattle begins to decrease; the feed intake of broilers aged 4 to 6 weeks at 32°C is 24% less than that at 22°C; when the ambient temperature exceeds 23.9°C, the feed intake and growth rate of pigs will decrease, and the acute heat stress causes a greater reduction in feed intake than the chronic heat stress. The acute heat stress feed intake reduction can reach 64%, and the chronic heat stress feed intake reduction is 36%. Stress has a great impact on the growth and economic value of livestock and poultry. Sports stress refers to stressors such as bumps, crowds, and noise during transportation that increase the secretion of stress hormones such as glucocorticoids in animals, which inhibits the animal's immune system, reduces the activity of the animal's immune cells, reduces antibody production, and weakens resistance to pathogens, making it more susceptible to infection. For example, the activity of pigs' lymphocytes will be significantly reduced after long-distance transportation, and the incidence of respiratory and digestive tract diseases will increase. Immune stress will cause the animal body to transfer more energy and nutrients from growth and production to immune defense. For example, after being infected with pathogens, the animal's body will initiate an immune response and produce immune-active substances such as cytokines. These substances will inhibit the secretion and action of growth hormones, resulting in a significant decrease in the animal's growth rate and slow weight gain.

[0003] In order to enhance the anti-stress ability of livestock and poultry, it is urgently needed to provide an anti-stress product. Summary of the invention

[0004] In order to enhance the anti-stress ability of livestock and poultry, the present invention provides an anti-stress oral liquid.

[0005] The technical solution adopted by the present invention is:

[0006] The present invention provides an anti-stress oral liquid, which is prepared from the following raw materials in parts by weight:

[0007] 138 to 142 parts of dried tangerine peel, 38 to 42 parts of jujube, 18 to 22 parts of Shenqu, 18 to 22 parts of Forsythia, 18 to 22 parts of Bupleurum, 18 to 22 parts of Isatis root, 18 to 22 parts of Isatis indigotica, 18 to 22 parts of Astragalus, 18 to 22 parts of Scutellaria, 18 to 22 parts of Codonopsis, 38 to 42 parts of Lycium barbarum, 18 to 22 parts of Atractylodes macrocephala, 38 to 42 parts of Dioscorea opposita, 18 to 22 parts of Angelica sinensis and 18 to 22 parts of Poria.

[0008] Preferably, the anti-stress oral liquid is made from the following raw materials in parts by weight:

[0009] 140 parts of dried tangerine peel, 40 parts of jujube, 20 parts of Shenqu, 20 parts of forsythia, 20 parts of northern bupleurum, 20 parts of isatis root, 20 parts of isatis indigotica, 20 parts of astragalus, 20 parts of scutellaria, 20 parts of codonopsis, 40 parts of wolfberry, 20 parts of atractylodes, 40 parts of yam, 20 parts of angelica and 20 parts of tuckahoe.

[0010] The invention provides an application of the anti-stress oral liquid, and the anti-stress oral liquid is used for preparing feed for resisting stress of livestock and poultry.

[0011] Preferably, the stress includes any one of cold stress, heat stress, transportation stress and immune stress.

[0012] Preferably, the anti-livestock stress comprises regulating the levels of IgG antibodies, IgA antibodies, IL-1β, TNF-α and IFN-γ in livestock and poultry.

[0013] Preferably, the livestock and poultry include any one of cattle, pigs, sheep, rabbits, chickens, ducks or geese.

[0014] Preferably, the feed further comprises pharmaceutically acceptable excipients.

[0015] Preferably, the pharmaceutically acceptable excipients include at least one of a diluent, a disintegrant, a precipitation inhibitor, a glidant, a binder, a dispersant, a suspending agent, an isotonic agent, a thickener, an emulsifier, a preservative and a stabilizer.

[0016] Preferably, the diluent includes any one of starch, lactose, sucrose and mannitol.

[0017] Preferably, the disintegrant includes at least one of starch, microcrystalline cellulose and low-substituted hydroxypropyl cellulose.

[0018] Preferably, the precipitation inhibitor includes any one of sodium lauryl sulfate, Tween-80, polyvinyl pyrrolidone and hydroxypropyl methylcellulose.

[0019] Preferably, the glidant includes any one of cationic polyacrylamide, polydiallyldimethylammonium chloride and cationic starch.

[0020] Preferably, the binder comprises any one of starch slurry, hydroxypropyl methylcellulose and povidone.

[0021] Preferably, the dispersant includes any one of sodium lauryl sulfate, polyvinyl pyrrolidone and sodium carboxymethyl cellulose.

[0022] Preferably, the suspending agent comprises any one of gum arabic, gum tragacanth, sodium carboxymethylcellulose and hydroxypropyl methylcellulose.

[0023] Preferably, the isotonic agent includes any one of sodium chloride, glucose and mannitol.

[0024] Preferably, the thickener includes any one of gum arabic, xanthan gum and sodium carboxymethyl cellulose.

[0025] Preferably, the emulsifier includes any one of sodium lauryl sulfate, benzalkonium chloride and sorbitan fatty acid.

[0026] Preferably, the preservative includes any one of benzoic acid, sorbic acid, methylparaben and benzalkonium bromide.

[0027] Preferably, the stabilizer comprises any one of sodium sulfite, sodium bisulfite, tocopherol and disodium edetate.

[0028] The present invention also provides a method for preparing the anti-stress oral liquid, comprising the following steps:

[0029] The raw materials of dried tangerine peel, jujube, Shenqu, forsythia, northern bupleurum, isatis root, isatis indigotica, astragalus, scutellaria, codonopsis, wolfberry, atractylodes, yam, angelica, and poria are weighed according to the weight proportions and set aside; the weighed raw materials are decocted with water and the drug decoction is collected; the drug decoction is concentrated, freeze-dried, and crushed to obtain an anti-stress powder; water is added to the anti-stress powder and mixed to obtain the anti-stress oral liquid.

[0030] Preferably, the ratio of the raw material drug to water is 450g-510g:4400mL, and the decoction conditions are high heat for 30 minutes and then low heat for 100 minutes.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The present invention provides an anti-stress oral liquid, which is prepared from the following raw materials in parts by weight:

[0033] 138 to 142 parts of dried orange peel, 38 to 42 parts of jujube, 18 to 22 parts of Shenqu, 18 to 22 parts of forsythia, 18 to 22 parts of northern bupleurum, 18 to 22 parts of isatis root, 18 to 22 parts of isatis indigotica, 18 to 22 parts of astragalus, 18 to 22 parts of scutellaria, 18 to 22 parts of codonopsis, 38 to 42 parts of wolfberry, 18 to 22 parts of atractylodes, 38 to 42 parts of yam, 18 to 22 parts of angelica and 18 to 22 parts of poria. The present invention takes the pure natural Chinese medicine anti-stress oral liquid as the starting point, and develops the anti-stress oral liquid that can be freely taken orally by livestock and poultry and enhances the anti-stress ability by improving the immunity of livestock and poultry.

[0034] In addition, this anti-stress oral liquid is suitable for a variety of animals, can effectively solve the subsequent diseases caused by stress in livestock and poultry, reduce labor costs, and promote the development of animal husbandry. The present invention establishes a mouse model of exercise stress, cold stress and heat stress. After exercise stress, the lungs of mice bleed due to excessive exercise. After taking the anti-stress oral liquid, the bleeding situation is improved, the inflammatory reaction disappears, and the concentrations change in a gradient. After stress, the weight of the mice is reduced, and the immunity of the mice is also reduced. After taking the anti-stress oral liquid, the weight recovery of the mice is better, and it is found that the anti-stress oral liquid has the ability to promote growth, and the serum IgA, lgG, IL-1β, TNF-α and IFN-γ are all restored to normal levels.

[0035] In order to explore the growth-promoting ability of the anti-stress oral liquid of the present invention, the present invention raised 30 chicks, took the anti-stress oral liquid orally every day, and observed the health status and weight indicators of the chicks. It was found that the anti-stress oral liquid has the effect of improving immunity, reducing the morbidity and mortality of chicks. In the heat stress experiment, the mortality rate of chicks with an oral drug concentration of 10 mg / mL was 30%, the mortality rate of chicks with an oral drug concentration of 1.25 mg / mL was 0%, and the mortality rate of chicks that did not take the oral liquid was 100%. And the anti-stress oral liquid has the ability to promote growth and can enhance the appetite of chickens. In a 30-day feeding experiment, the weight of chicks with an oral drug concentration of 10 mg / mL increased by an average of 471.9g, the weight of chicks with an oral drug concentration of 1.25 mg / mL increased by an average of 466.3g, and the weight of chicks that did not take the oral liquid increased by an average of 331.75g. Microscopic examination of the breast muscles of each group of chicks revealed that the muscle tissue structure of the control group was loose, while the high-concentration muscle tissue was arranged neatly and compactly, which shows that the anti-stress oral liquid can not only promote growth but also improve meat quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1These are the staining results of the mouse exercise stress model. A: The drug concentration was 10 mg / mL, and paraffin sections were prepared from the mouse lungs; B: The drug concentration was 5 mg / mL, and paraffin sections were prepared from the mouse lungs; C: The drug concentration was 2.5 mg / mL, and paraffin sections were prepared from the mouse lungs; D: The drug concentration was 1.25 mg / mL, and paraffin sections were prepared from the mouse lungs; E: The drug concentration was 10 mg / mL, and paraffin sections were prepared from the mouse spleen; F: The drug concentration was 5 mg / mL, and paraffin sections were prepared from the mouse spleen; G: The drug concentration was 2.5 mg / mL, and paraffin sections were prepared from the mouse spleen; H: The drug concentration was 1.25 mg / mL, and paraffin sections were prepared from the mouse spleen.

[0037] Figure 2 To study the effect of anti-stress oral liquid on body weight changes in mice induced by exercise stress.

[0038] Figure 3 To study the effect of anti-stress oral liquid on the level of IgG antibodies produced in the blood of mice induced by exercise stress.

[0039] Figure 4 To study the effect of anti-stress oral liquid on the level of IgA antibody production in the blood of mice induced by exercise stress.

[0040] Figure 5 To study the effect of anti-stress oral liquid on the production level of interleukin IL-1β in the blood of mice induced by exercise stress.

[0041] Figure 6 To study the effect of anti-stress oral liquid on the production level of interleukin TNF-α in the blood of mice induced by exercise stress.

[0042] Figure 7 To study the effect of anti-stress oral liquid on the level of interleukin IFN-γ in the blood of mice induced by exercise stress.

[0043] Figure 8 The heat stress model of mice was established.

[0044] Fig. 9 Effect of anti-stress oral liquid on body weight changes in heat stressed mice.

[0045] Fig.10 To study the effect of anti-stress oral liquid on the level of IgG antibody production in the blood of heat stressed mice.

[0046] Fig.11 To study the effect of anti-stress oral liquid on the level of IgA antibody production in the blood of heat stressed mice.

[0047] Fig.12 To study the effect of anti-stress oral liquid on the production level of interleukin IL-1β in the blood of heat stressed mice.

[0048] Fig.13 To study the effect of anti-stress oral liquid on the production level of interleukin TNF-α in the blood of heat stressed mice.

[0049] Fig.14 To study the effect of anti-stress oral liquid on the production level of interleukin TNF-γ in the blood of heat stressed mice.

[0050] Fig.15 The mouse cold stress model was established.

[0051] Fig.16 Effect of anti-stress oral liquid on body weight changes in cold-stressed mice.

[0052] Fig.17 To study the effect of anti-stress oral liquid on the level of IgG antibodies produced in the blood of cold-stressed mice.

[0053] Fig.18 To study the effect of anti-stress oral liquid on the level of IgA antibody production in the blood of cold-stressed mice.

[0054] Fig.19 To study the effect of anti-stress oral liquid on the production level of interleukin IL-1β in the blood of cold-stressed mice.

[0055] Fig. 20 To study the effect of anti-stress oral liquid on the production level of interleukin TNF-α in the blood of cold-stressed mice.

[0056] Fig.21 To study the effect of anti-stress oral liquid on the level of interleukin IFN-γ production in the blood of cold-stressed mice.

[0057] Fig. 22 To study the effect of anti-stress oral liquid on the growth and weight changes of chicks.

[0058] Fig.23 To study the effect of anti-stress oral liquid on changes in organ indexes of chicks.

[0059] Fig.24 To study the effect of anti-stress oral liquid on the resistance of chicks to heat stress.

[0060] Fig.25 To show the effect of anti-stress oral liquid on the muscle quality of chicks, A to C are the results after feeding 0 mg / mL, 1.25 mg / mL and 10 mg / mL of anti-stress oral liquid, respectively. DETAILED DESCRIPTION

[0061] The present invention is further described below by specific examples, but the scope of the present invention is not limited thereto. The details and forms of the technical solution of the present invention may be modified or replaced without departing from the spirit and scope of the present invention, but these modifications or replacements all fall within the protection scope of the present invention.

[0062] The inventive concept of the present invention is as follows:

[0063] When livestock and poultry are subjected to stress such as cold stress, heat stress, transportation stress and immune stress, their bodies will have nonspecific systemic reactions, which will have a serious impact on the animals' feed intake and production performance.

[0064] In order to enhance the anti-stress ability of livestock and poultry, the present invention provides an anti-stress oral liquid, which is prepared from the following raw materials in parts by weight:

[0065] 138 to 142 parts of dried tangerine peel, 38 to 42 parts of jujube, 18 to 22 parts of Shenqu, 18 to 22 parts of Forsythia, 18 to 22 parts of Bupleurum, 18 to 22 parts of Isatis root, 18 to 22 parts of Isatis indigotica, 18 to 22 parts of Astragalus, 18 to 22 parts of Scutellaria, 18 to 22 parts of Codonopsis, 38 to 42 parts of Lycium barbarum, 18 to 22 parts of Atractylodes macrocephala, 38 to 42 parts of Dioscorea opposita, 18 to 22 parts of Angelica sinensis and 18 to 22 parts of Poria.

[0066] The main drugs in the raw materials of the anti-stress oral liquid of the present invention are codonopsis, astragalus, jujube and wolfberry; the assistant drugs are isatis root, isatis indigotica, scutellaria, forsythia and bupleurum; the adjuvant drugs are atractylodes, yam, angelica and tuckahoe; the guiding drugs are tangerine peel and Shenqu. This prescription is mainly used to tonify the middle qi and strengthen the body, supplemented by the effects of clearing away heat and detoxification, relieving exterior symptoms and dispersing cold, strengthening the spleen and removing dampness, nourishing blood and regulating qi, promoting the gastrointestinal transport and transformation function of the body, improving the body's immunity and anti-inflammatory ability, and achieving the effect of disease resistance and growth promotion.

[0067] In order to enable those skilled in the art to better understand the technical solution of the present invention and implement it, the present invention is further described below in conjunction with specific embodiments. In the description of the present invention, unless otherwise specified, the reagents used are all commercially available, and the methods used are all conventional techniques in the art.

[0068] In the following examples, 1 g represents one part by weight.

[0069] Example 1

[0070] An anti-stress oral liquid is prepared from the following raw materials in parts by weight:

[0071] Tangerine peel 140g, jujube 40g, Shenqu 20g, Forsythia suspensa 20g, Bupleurum 20g, Isatis root 20g, Isatis indigotica 20g, Astragalus 20g, Scutellaria baicalensis 20g, Codonopsis pilosula 20g, Lycium barbarum 40g, Atractylodes macrocephala 20g, Chinese yam 40g, Angelica sinensis 20g and Poria 20g.

[0072] Example 2

[0073] An anti-stress oral liquid is prepared from the following raw materials in parts by weight:

[0074] Tangerine peel 138g, jujube 38g, Shenqu 18g, Forsythia suspensa 18g, Bupleurum chinense 18g, Isatis root 18g, Isatis indigotica 18g, Astragalus 18g, Scutellaria baicalensis 18g, Codonopsis pilosula 18g, Lycium barbarum 38g, Atractylodes macrocephala 18g, Chinese yam 38g, Angelica sinensis 18g and Poria cocos 18g.

[0075] Example 3

[0076] An anti-stress oral liquid is prepared from the following raw materials in parts by weight:

[0077] Tangerine peel 142g, jujube 42g, Shenqu 22g, Forsythia suspensa 22g, Bupleurum 22g, Isatis root 22g, Isatis indigotica 22g, Astragalus 22g, Scutellaria baicalensis 22g, Codonopsis pilosula 22g, Lycium barbarum 42g, Atractylodes macrocephala 22g, Chinese yam 42g, Angelica sinensis 22g and Poria 22g.

[0078] Example 4

[0079] A method for preparing an anti-stress oral liquid comprises the following steps:

[0080] The anti-stress oral liquids described in Examples 1 to 3 above are all prepared according to the following steps:

[0081] Weigh tangerine peel, jujube, Shenqu, forsythia, northern bupleurum, isatis root, isatis indigotica, astragalus, scutellaria, codonopsis, wolfberry, atractylodes, yam, angelica and poria, add 4400mL of water, first boil over high heat for 30 minutes and then boil over low heat for 100 minutes to make a dark brown liquid, collect the drug decoction, pour it into a rotary evaporator for concentration, concentrate it 10 times, pour the water extract into a disposable culture dish, place it in a freeze dryer for freeze drying for 40 hours, and finally grind it into powder with a mortar to obtain anti-stress powder, place it in -20°C for freezing and storage, and dissolve the anti-stress powder in water to obtain an anti-stress oral solution.

[0082] Example 5

[0083] An application of an anti-stress oral liquid is as follows:

[0084] The following tests were all conducted using the anti-stress oral liquid prepared using the raw material drug of Example 1.

[0085] 1. The evaluation of the effect of anti-stress oral solution on combating exercise stress is as follows:

[0086] 1.1. Establishment of mouse exercise stress model.

[0087] Twenty-one mice were selected and divided into six groups, including five drug-treated groups and one blank group. During the entire experimental period, all mice had free access to feed. The six groups of mice were placed on a mouse treadmill to establish a three-day exercise stress model, with a duration of 2 hours and a speed of 14 m / min. Body weight was monitored daily. After the exercise stress was completed, anti-stress oral liquid was administered with free drinking water. The concentrations were 10 mg / mL, 5 mg / mL, 2.5 mg / mL, 1.25 mg / mL and 0.625 mg / mL, respectively. They were recorded as T1 group, T2 group, T3 group, T4 group and T5 group, respectively, for a period of 15 days. Body weight was monitored daily. After 15 days, mice in groups T1, T2, T3, T4 and T5 were autopsied and slices were made. Figure 1 As shown, the alveolar walls of mice in each group were thickened, T2 and T3 mice had slight lung hemorrhage, and T4 mice had severe lung hemorrhage. There were serous exudates in the bronchi of mice in the T3 and T4 groups, and the lymphoid follicles were enlarged. The alveolar structure of the T4 group disappeared and necrotized, and inflammatory cells appeared. The size of the lymph nodules in the spleen tissue of T1 was uniform, and the individuals were larger, showing enhanced immunity. The size of the lymph nodules in the spleen tissue of T3 and T4 mice was uneven, showing reduced immunity.

[0088] Figure 1 A. Thickening of alveolar walls; Figure 1 B has thickened alveolar walls and slight hemorrhage; Figure 1 C shows thickening of alveolar walls, hemorrhage, serous exudate, and enlarged lymphoid follicles; Figure 1 D. The alveolar structure disappears and becomes necrotic, and there is serous exudate in the bronchi and the appearance of inflammatory cells; Figure 1 The lymph nodes in spleen tissues of E and F were uniform in size and larger in size; Figure 1 The G and H splenic lymph nodes are unevenly distributed in size.

[0089] 1.2. Effect of anti-stress oral liquid on body weight changes in exercise-stressed mice.

[0090] After the stress, the average weight of the mice decreased. After 4 days of administration, the weight of each group increased. The weight of the mice was counted and a line graph was drawn. The line comparison results are shown in the figure below. Figure 2 It can be clearly concluded that the anti-stress oral liquid has the ability to resist stress.

[0091] 1.3. Effect of anti-stress oral liquid on the level of IgG antibody production in the blood of mice induced by exercise stress.

[0092] like Figure 3 As shown, the IgG antibodies of mice increased significantly after exercise stress, and the IgG content in each group decreased after administration. The anti-stress effect was most obvious when the concentration of the anti-stress oral solution was 5 mg / mL. Figure 3 ns means no significant difference, P>0.05.

[0093] 1.4. Effect of anti-stress oral liquid on the level of IgA antibody production in the blood of mice induced by exercise stress.

[0094] like Figure 4 As shown, there was no significant change in the level of IgA antibody production in the blood of mice before and after exercise stress and before and after drug administration. Figure 4 The * indicates significant difference, P<0.05.

[0095] 1.5. Effect of anti-stress oral liquid on the production level of interleukin IL-1β in the blood of mice with exercise stress.

[0096] like Figure 5 As shown, the interleukin IL-1β in the blood of mice increased significantly after exercise stress, and the blood interleukin IL-β1 content in each group decreased after administration. The effect was obvious when the concentration of the anti-stress oral solution was 5 mg / mL. Figure 5 The * indicates significant difference, P<0.05.

[0097] 1.6. Effect of anti-stress oral liquid on the production level of interleukin TNF-α in the blood of mice with exercise stress.

[0098] like Figure 6 As shown, interleukin TNF-α in the blood of mice increased significantly after exercise stress, and the interleukin TNF-α content in the blood of each group continued to increase after administration. Figure 6 ns means no significant difference, P>0.05.

[0099] 1.7. Effect of anti-stress oral liquid on the level of interleukin IFN-γ production in the blood of mice with exercise stress.

[0100] like Figure 7 As shown, the blood interleukin IFN-γ of mice increased significantly after exercise stress, and the blood interleukin IFN-γ content of each group decreased after administration. The effect was obvious when the concentration of the anti-stress oral solution was 5 mg / mL. Figure 7 ns means no significant difference, P>0.05.

[0101] 2. The effect of anti-stress oral liquid on heat stress is evaluated as follows:

[0102] 2.1. Establishment of heat stress model in mice.

[0103] Eight mice were selected and divided into two groups, an experimental group and a control group. During the entire experimental period, the mice had free access to feed. Before the heat stress experiment, the experimental group was given an anti-stress oral solution with a concentration of 10 mg / mL every day until the end of the experiment, and the mice in the control group drank water normally. Five days after administration, the two groups of mice were placed in a 37°C oven for 4 days, lasting 1 hour and 30 minutes each day. The health of the mice and the weight of the mice were observed daily, and the mice in the control group died during the heat stress experiment on the fourth day. The mice in the control group showed fatigue and sweating, and were scattered throughout the cage. Figure 8 .

[0104] 2.2. Effect of anti-stress oral liquid on body weight changes in heat stressed mice.

[0105] like Fig. 9 As shown, the weight of mice in the experimental group was relatively stable, while the weight of mice in the control group was unstable, with large increases or decreases.

[0106] 2.3. Effect of anti-stress oral liquid on the level of IgG antibody production in the blood of heat stressed mice.

[0107] like Fig.10 As shown, IgG is one of the most important antibodies in the body, which can neutralize viruses and fight bacteria. Usually, the level of IgG antibody production in the blood of mice will increase after heat stress, indicating that there is an inflammatory response in the mice. In the present invention, the level of IgG antibody production in the experimental group is decreasing, indicating that the inflammatory response in the body is reduced. Fig.10 ns means no significant difference, P>0.05; * means there is a significant difference, P<0.05.

[0108] 2.4. Effect of anti-stress oral liquid on the level of IgA antibody production in the blood of heat stressed mice.

[0109] like Fig.11 As shown, the level of IgA antibodies in the control group decreased after heat stress in mice, and the immunity of mice decreased due to the influence of heat stress (p < 0.01), while the level of IgA production in the experimental group increased. Fig.11 ns means no significant difference, P>0.05; ** means there is a significant difference, P<0.01.

[0110] 2.5. Effect of anti-stress oral liquid on the level of interleukin IL-β1 production in the blood of heat stressed mice.

[0111] like Fig.12 As shown, the level of interleukin IL-1β production in the blood of mice increased significantly after heat stress, p < 0.05. Although the level of interleukin IL-1β production in the experimental group also increased, the increase was not significant, p > 0.05. Fig.12 ns means no significant difference, P>0.05; * means there is a significant difference, P<0.05.

[0112] 2.6. Effect of anti-stress oral liquid on the level of interleukin TNF-α production in the blood of heat stressed mice.

[0113] like Fig.13 As shown, the blood interleukin TNF-α of mice increased significantly after heat stress, and the blood interleukin TNF-α content of each group continued to increase after administration, but the increase was not significant. Fig.13 ns means no significant difference, P>0.05; * means there is a significant difference, P<0.05.

[0114] 2.7. Effect of anti-stress oral liquid on the level of interleukin IFN-γ production in the blood of heat stressed mice.

[0115] like Fig.14 As shown, the blood interleukin IFN-γ of mice increased significantly after heat stress, and the blood interleukin IFN-γ content of each group decreased after administration. Fig.14 ns means no significant difference, P>0.05.

[0116] 3. The evaluation of the effect of anti-stress oral solution on anti-cold stress is as follows:

[0117] 3.1. Establishment of cold stress model in mice.

[0118] Eight mice were selected and divided into two treatment groups, one experimental group and one control group, with 4 mice in each group. During the entire experimental period, mice had free access to feed. Before the cold stress experiment, the experimental group was given an anti-stress oral solution with a concentration of 10 mg / mL every day until the end of the experiment, and the mice in the control group drank water normally. Five days after administration, the two groups of mice were placed in a 4°C refrigerator for 4 days, for 2 hours a day. The health of the mice was observed and their weight was monitored daily. The mice in the control group and the experimental group showed shivering, their arrector pili muscles contracted and their bodies curled up. The mice curled up in the corner of the cage. Fig.15 .

[0119] 3.2. Effect of anti-stress oral liquid on body weight changes in cold-stressed mice.

[0120] The weight of mice in both the control group and the experimental group decreased due to cold stress, but the weight of the experimental group began to recover on the 3rd day, and the weight of the experimental group began to recover on the 4th day. Fig.16 .

[0121] 3.3. Effect of anti-stress oral liquid on the level of IgG antibody production in the blood of cold-stressed mice.

[0122] like Fig.17 As shown, the level of IgG antibody production in the blood of mice decreased after cold stress, while the level of IgG antibody production in the experimental group did not decrease significantly. Fig.17ns means no significant difference, P>0.05.

[0123] 3.4. Effect of anti-stress oral liquid on the level of IgA antibody production in the blood of cold-stressed mice.

[0124] like Fig.18 As shown, the level of IgA antibody production in the blood of mice increased significantly after cold stress, P < 0.05, and the level of IgA antibody production in the blood did not increase significantly after administration, P > 0.05. Fig.18 ns means no significant difference, P>0.05; * means there is a significant difference, P<0.05.

[0125] 3.5. Effect of anti-stress oral liquid on the production level of interleukin IL-1β in the blood of cold-stressed mice.

[0126] like Fig.19 As shown, the level of interleukin IL-1β production in the blood of mice increased after cold stress, and the level of interleukin IL-β1 production in the experimental group also increased. Fig.19 ns means no significant difference, P>0.05.

[0127] 3.6. Effect of anti-stress oral liquid on the production level of interleukin TNF-α in the blood of cold-stressed mice.

[0128] like Fig. 20 As shown, the blood interleukin TNF-α of mice decreased after cold stress, and the blood interleukin TNF-α content of each group still decreased after drug administration but not significantly. Fig. 20 ns means no significant difference, P>0.05.

[0129] 3.7 Effect of anti-stress oral liquid on the level of interleukin IFN-γ production in the blood of cold-stressed mice.

[0130] like Fig.21 As shown, the blood interleukin IFN-γ of mice was significantly reduced after cold stress, and the blood interleukin IFN-γ content of each group did not decrease significantly after drug administration. Fig.21 ns means no significant difference, P>0.05.

[0131] 4. The evaluation of the growth-promoting effect of anti-stress oral liquid is as follows:

[0132] 4.1. Effect of anti-stress oral liquid on growth and weight changes of chicks.

[0133] Thirty chicks were selected and divided into three treatment groups to receive normal feed every day. The three treatment groups were given 200 mL of anti-stress oral solution with concentrations of 10 mg / mL, 1.25 mg / mL and 0 mg / mL every day, and the weight of the chickens was tested every 5 days, as shown in Table 1.

[0134] Table 1 Effect of anti-stress oral liquid on growth and weight changes of chicks

[0135] project 10mg / mL 1.25mg / mL 0mg / mL Average first weight / g 40.7 37.9 38.8 Weight gain after 5 days / g 31 26.3 9.4 Weight gain after 10 days / g 50.99 57.5 28.84 Weight gain after 15 days / g 59.41 69.3 1.21 Weight gain after 20 days / g 29.3 21.4 73.7 Weight gain after 25 days / g 199.3 199.6 89.2 Weight gain after 30 days / g 134.3 124.5 129.4 Average final weight / g 512.6 504.3 370.55 Average final weight - average initial weight / g 471.9 466.3 331.75

[0136] Within 15 days, the anti-stress oral liquid with a concentration of 1.25 mg / mL has a better growth-promoting effect, and the anti-stress oral liquid with a concentration of 10 mg / mL has a better growth-promoting effect within 15 days to 20 days. According to the average final weight minus the average first weight, the anti-stress oral liquid with a concentration of 10 mg / mL has a better growth-promoting effect. Fig. 22 .

[0137] 4.2. Effect of anti-stress oral liquid on changes in organ indexes of chicks.

[0138] After 30 days, the chickens were dissected, and the heart, liver, spleen, and lungs were taken out and the excess fat, blood, and water in the organs were removed with filter paper. The organ coefficients were then weighed and the organ coefficients were calculated. As shown in the figure, the organ coefficients of the heart, liver, spleen, and lungs with an anti-stress oral solution concentration of 1.25 mg / mL were all greater than 10 mg / mL. Fig.23 . Fig.23 ns means no significant difference, P>0.05; ** means there is a significant difference, P<0.01.

[0139] 4.3. Effect of anti-stress oral liquid on the resistance of chicks to heat stress.

[0140] The chicks were placed outdoors in sunlight for about four hours at a temperature of about 29°C. All chicks in the control group died of heat stress, while none of the chicks that took the anti-stress oral solution at a concentration of 1.25 mg / mL died. Three chicks that took the anti-stress oral solution at a concentration of 10 mg / mL died. Fig.24 .

[0141] 4.4. Effect of anti-stress oral liquid on muscle quality of chicks.

[0142] Take the muscle tissue of each group for cross-section microscopy. Fig.25 The structure between muscle fibers in the control group was loose, and the distance between muscle bundles was large. The structure between muscle fibers with a drug concentration of 1.25 mg / mL was slightly tighter, and the distance between muscle bundles was slightly smaller. The structure between muscle fibers and muscle bundles with a drug concentration of 10 mg / mL was compact.

[0143] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0144] The above-mentioned embodiments only express several implementation methods of the present invention, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the invention patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. An anti-stress oral liquid, characterized in that: The anti-stress oral liquid is prepared from the following raw materials in parts by weight: 138 to 142 parts of dried tangerine peel, 38 to 42 parts of jujube, 18 to 22 parts of Shenqu, 18 to 22 parts of Forsythia, 18 to 22 parts of Bupleurum, 18 to 22 parts of Isatis root, 18 to 22 parts of Isatis indigotica, 18 to 22 parts of Astragalus, 18 to 22 parts of Scutellaria, 18 to 22 parts of Codonopsis, 38 to 42 parts of Lycium barbarum, 18 to 22 parts of Atractylodes macrocephala, 38 to 42 parts of Dioscorea opposita, 18 to 22 parts of Angelica sinensis and 18 to 22 parts of Poria.

2. The anti-stress oral liquid according to claim 1, characterized in that: The anti-stress oral liquid is prepared from the following raw materials in parts by weight: 140 parts of dried tangerine peel, 40 parts of jujube, 20 parts of Shenqu, 20 parts of forsythia, 20 parts of northern bupleurum, 20 parts of isatis root, 20 parts of isatis indigotica, 20 parts of astragalus, 20 parts of scutellaria, 20 parts of codonopsis, 40 parts of wolfberry, 20 parts of atractylodes, 40 parts of yam, 20 parts of angelica and 20 parts of tuckahoe.

3. The use of the anti-stress oral liquid as claimed in claim 1, characterized in that: The anti-stress oral liquid is used for preparing feed for resisting stress of livestock and poultry.

4. The use according to claim 3, characterized in that The stress includes any one of cold stress, heat stress, transportation stress and immune stress.

5. The use according to claim 3, characterized in that The anti-livestock and poultry stress comprises regulating the contents of IgG antibodies, IgA antibodies, IL-1β, TNF-α and IFN-γ of livestock and poultry.

6. The use according to claim 3, characterized in that The livestock and poultry include any one of cattle, pigs, sheep, rabbits, chickens, ducks and geese.

7. The use according to claim 3, characterized in that The feed also includes pharmaceutically acceptable adjuvants.

8. The use according to claim 7, characterized in that The pharmaceutically acceptable excipients include at least one of a diluent, a disintegrant, a precipitation inhibitor, a glidant, a binder, a dispersant, a suspending agent, an isotonic agent, a thickener, an emulsifier, a preservative and a stabilizer.

9. The method for preparing the anti-stress oral liquid according to claim 1, characterized in that: The following steps are involved: Weigh the raw materials of dried tangerine peel, jujube, Shenqu, forsythia, northern bupleurum, isatis root, isatis leaf, astragalus, scutellaria, codonopsis, wolfberry, atractylodes, yam, angelica, and poria according to the weight parts, and set aside; The weighed raw material drug is boiled with water, and the drug decoction is collected; The drug decoction is concentrated, freeze-dried, and crushed to obtain anti-stress powder; The anti-stress powder is added with water and mixed evenly to obtain the anti-stress oral liquid.

10. The preparation method according to claim 9, characterized in that: The ratio of the raw material medicine to water is 450g-510g:4400mL, and the decoction condition is first high heat for 30 minutes and then low heat for 100 minutes.

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