Composite polyester fiber cloth, method for preparing the same, and use thereof

Abstract

This invention relates to the field of fiber fabric technology, and discloses a composite polyester fiber fabric, its preparation method, and its application. The composite polyester fiber fabric comprises a first polyester fiber layer and a second polyester fiber layer, which are alternately stacked. The number of first polyester fiber layers is n, and the number of second polyester fiber layers is n-1, where n≥2. The melting point of the first polyester fiber in the first polyester fiber layer is at least 20°C higher than the melting point of the second polyester fiber in the second polyester fiber layer. When the composite polyester fiber fabric of this invention is used for cell culture, it can significantly improve the glycogen metabolism capacity of cells.

Description

Technical Field

[0001] This invention relates to the field of fiber fabric technology, specifically to a composite polyester fiber fabric, its preparation method, and its application. Background Technology

[0002] With the increasing demand for cell culture-based biopharmaceuticals such as protein drugs and vaccines, large-scale cell culture technology has been continuously developed. Cell culture refers to the process of enabling cells to survive, grow, and reproduce in vitro, mimicking the environment inside a living organism. Currently, cell culture mainly includes adherent culture technology (e.g., roller bottle culture), suspension culture technology, and immobilized culture (e.g., microcarrier culture). Among these, microcarriers used in microcarrier culture provide an attachment and growth surface for adherent-dependent cells, making them an important functional material in large-scale cell culture. Currently, most cell culture processes use porous microspheres as carriers. The pores in porous microspheres are conducive to cell adhesion and growth; however, as the number of cells increases, these pores are easily blocked, affecting mass transfer between cells and the culture medium. Summary of the Invention

[0003] The purpose of this invention is to overcome the problem that cell culture carriers in the prior art have poor performance when used for cell culture, and to provide a composite polyester fiber cloth, its preparation method and application.

[0004] To achieve the above objectives, the first aspect of the present invention provides a composite polyester fiber fabric comprising a first polyester fiber layer and a second polyester fiber layer, wherein the first polyester fiber layer and the second polyester fiber layer are alternately stacked, the number of layers of the first polyester fiber layer is n, the number of layers of the second polyester fiber layer is n-1, and n≥2, wherein the melting point of the first polyester fiber in the first polyester fiber layer is more than 20°C higher than the melting point of the second polyester fiber in the second polyester fiber layer.

[0005] The second aspect of the present invention provides a method for preparing a composite polyester fiber cloth, the method comprising: alternately laying a first polyester fiber and a second polyester fiber in a web, and then hot-pressing and bonding them; wherein the number of layers of the first polyester fiber web is n, the number of layers of the second polyester fiber web is n-1, n≥2, and the melting point of the first polyester fiber is more than 20°C higher than the melting point of the second polyester fiber.

[0006] The third aspect of the present invention provides a composite polyester fiber fabric prepared by the method described above.

[0007] The fourth aspect of the present invention provides the application of the composite polyester fiber cloth described above and / or the composite polyester fiber cloth prepared by the method described above in cell culture.

[0008] When the composite polyester fiber cloth of the present invention is used for cell culture, it can significantly improve the sugar metabolism capacity of cells. The higher the sugar metabolism capacity, the higher the cell proliferation rate and survival rate (only living cells can metabolize sugar, and dead cells cannot metabolize sugar). That is, the composite polyester fiber cloth can achieve better results when used for cell culture. Detailed Implementation

[0009] The endpoints and any values ​​of the ranges disclosed herein are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of the various ranges, the endpoint values ​​of the various ranges and individual point values, and individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed herein.

[0010] The first aspect of the present invention provides a composite polyester fiber fabric, which includes a first polyester fiber layer and a second polyester fiber layer, wherein the first polyester fiber layer and the second polyester fiber layer are alternately stacked, the number of the first polyester fiber layer is n layers, the number of the second polyester fiber layer is n-1 layers, and n≥2, wherein the melting point of the first polyester fiber in the first polyester fiber layer is more than 20°C higher than the melting point of the second polyester fiber in the second polyester fiber layer.

[0011] In this invention, both the upper and lower surfaces of the composite polyester fiber fabric are first polyester fiber layers.

[0012] According to the present invention, preferably, n is any integer from 2 to 10, for example, n is 2, 3, 4, 5, 6, 7, 8, 9, 10.

[0013] According to the present invention, preferably, the composite polyester fiber fabric has a thickness of 0.4-0.5 mm and an areal density of 80-130 g / m³. 2 .

[0014] According to the present invention, preferably, the first polyester fiber and the second polyester fiber are each independently made of polyethylene terephthalate, more preferably, the intrinsic viscosity of the polyethylene terephthalate is 0.6-0.85 dL / g, and the standard deviation of the intrinsic viscosity is ≤0.05 dL / g.

[0015] In this invention, the intrinsic viscosity of polyester is tested using the intrinsic viscosity test method described in GB / T 14190-2017. Specifically, the standard deviation of the intrinsic viscosity is tested as follows: six samples are randomly selected, and their intrinsic viscosity is tested according to the intrinsic viscosity test method described in GB / T 14190-2017. The standard deviation of the values ​​is then recorded. A smaller standard deviation of the intrinsic viscosity indicates higher uniformity of the intrinsic viscosity within the same batch of material.

[0016] According to the present invention, preferably, the material of the first polyester fiber is homopolymer polyethylene terephthalate. More preferably, the intrinsic viscosity of the homopolymer polyethylene terephthalate is 0.65-0.75 dL / g (for example, 0.65 dL / g, 0.66 dL / g, 0.67 dL / g, 0.68 dL / g, 0.69 dL / g, 0.70 dL / g, 0.71 dL / g, 0.72 dL / g, 0.73 dL / g, 0.74 dL / g, 0.75 dL / g, and any two of the above ranges), and even more preferably 0.65-0.7 dL / g.

[0017] According to the present invention, preferably, the homopolymer polyethylene terephthalate has a melting point of 220°C or higher (e.g., 220°C, 230°C, 240°C, 245°C, 248°C, 250°C, 255°C, 260°C, 265°C, 270°C, 280°C, 290°C, 300°C, 320°C, 350°C, 380°C, and any two of the above), more preferably 220-270°C, and even more preferably 255-265°C.

[0018] According to the present invention, preferably, the standard deviation of the intrinsic viscosity of the homopolymer polyethylene terephthalate is ≤0.01 dL / g, more preferably ≤0.005 dL / g, for example 0.003-0.005 dL / g.

[0019] According to the present invention, preferably, the material of the second polyester fiber is polyethylene terephthalate; more preferably, the intrinsic viscosity of the polyethylene terephthalate is 0.7-0.85 dL / g (for example, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.80 dL / g, 0.82 dL / g, 0.84 dL / g, 0.85 dL / g, and any two of the above ranges), and even more preferably 0.75-0.81 dL / g.

[0020] According to the present invention, preferably, the melting point of the copolymerized polyethylene terephthalate is below 220°C (e.g., 220°C, 200°C, 190°C, 180°C, 170°C, 160°C, 150°C, 145°C, 140°C, 135°C, 130°C, 120°C, and any two of the above), more preferably 120-200°C.

[0021] According to the present invention, preferably, the standard deviation of the intrinsic viscosity of the copolymer ethylene terephthalate is ≤0.015 dL / g, more preferably ≤0.01 dL / g, for example 0.005-0.006 dL / g.

[0022] According to the present invention, preferably, the copolymerized ethylene terephthalate comprises structural units derived from terephthalic acid, ethylene glycol, and comonomer Y, wherein the content of the structural units derived from comonomer Y in the copolymerized ethylene terephthalate is 0.6-18 mol% (e.g., 0.6 mol%, 0.75 mol%, 1 mol%, 1.5 mol%, 3 mol%, 5 mol%, 10 mol%, 15 mol%, 18 mol%, and any two of the above), more preferably 0.7-15 mol%. In the present invention, the content of structural units of comonomer Y refers to the percentage of the amount of substance of the structural units of comonomer Y relative to the total amount of substance of the structural units of terephthalic acid, ethylene glycol, and comonomer Y.

[0023] According to the present invention, the type of comonomer is not particularly limited, as long as the difference in melting point and intrinsic viscosity between the skin material and the core material, as well as the standard deviation of the intrinsic viscosity, meet the requirements. Preferably, the comonomer Y is selected from at least one of dicarboxylic acids, diols, and tetraols. More preferably, the dicarboxylic acid is a dicarboxylic acid containing a benzene ring, and further preferably isophthalic acid and / or phthalic acid. More preferably, the diol is a diol with 3-20 carbon atoms, and further preferably at least one of butanediol, hexanediol, cyclohexanediol, and pentanediol (e.g., neopentanediol). More preferably, the tetraol is a tetraol with 3-20 carbon atoms. In the present invention, the number of carbon atoms of the diol or tetraol can be independently 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, or 20.

[0024] In this invention, the comonomer Y may also be selected from acid monomers in other forms such as acid anhydrides, acyl chlorides, methanol esters, and ethanol esters, and alcohol monomers include alcohol monomers in other forms such as ethers, hemiacetals, and acetals.

[0025] According to the present invention, preferably, the Sb content in the materials of the first polyester fiber and the second polyester fiber is ≤80ppm. In the present invention, "ppm" refers to the content by weight.

[0026] According to the present invention, preferably, the first polyester fiber and the second polyester fiber each independently satisfy the following: average diameter of 15-35 μm and diameter standard deviation ≤2 μm.

[0027] In this invention, the average diameter of the first polyester fiber and the second polyester fiber can each independently be 15μm, 18μm, 20μm, 22μm, 24μm, 26μm, 28μm, 30μm, 32μm, 35μm, or any range formed by any two of the above points. The standard deviation of the diameter of the first polyester fiber and the second polyester fiber can each independently be 2μm, 1.8μm, 1.6μm, 1.4μm, 1.2μm, 1μm, 0.8μm, 0.6μm, 0.4μm, 0.2μm, 0.1μm, or any range formed by any two of the above points, for example, 0.1-2μm.

[0028] In this invention, the method for testing the standard deviation of diameter is as follows: Take 50 composite polyester fibers, randomly cut a small section from each fiber, take a scanning electron microscope (SEM) image, and measure the diameter of each fiber from the image to an accuracy of 0.1 μm. Then, calculate the standard deviation of these diameter values ​​according to the standard deviation calculation method.

[0029] In this invention, the first polyester fiber and the second polyester fiber refer to polyester fibers with a length greater than 150 mm.

[0030] The second aspect of the present invention provides a method for preparing a composite polyester fiber cloth, the method comprising: alternately laying a first polyester fiber and a second polyester fiber in a web, and then hot-pressing and bonding them; wherein the number of layers of the first polyester fiber web is n, the number of layers of the second polyester fiber web is n-1, n≥2, and the melting point of the first polyester fiber is more than 20°C higher than the melting point of the second polyester fiber.

[0031] According to the present invention, preferably, n is any integer from 2 to 10, for example, n is 2, 3, 4, 5, 6, 7, 8, 9, 10.

[0032] According to the present invention, preferably, the thickness of each single layer of the first polyester fiber web is 0.08-0.35 mm, more preferably 0.1-0.3 mm.

[0033] According to the present invention, preferably, when the number of layers of the first polyester fiber web is 2, the thickness of each single layer of the first polyester fiber web is independently 0.25-0.3 mm.

[0034] According to the present invention, preferably, when the number of layers of the first polyester fiber web is 3, the thickness of each single layer of the first polyester fiber web is independently 0.1-0.15 mm.

[0035] According to the present invention, preferably, when the number of layers of the first polyester fiber web is 4, the thickness of each single layer of the first polyester fiber web is independently 0.08-0.1 mm.

[0036] According to the present invention, preferably, the thickness of each single layer of the second polyester fiber web is 0.08-0.6 mm, more preferably 0.2-0.5 mm.

[0037] According to the present invention, preferably, when the number of layers of the second polyester fiber web is 1, the thickness of each single layer of the second polyester fiber web is independently 0.4-0.5 mm.

[0038] According to the present invention, preferably, when the number of layers of the second polyester fiber web is 2, the thickness of each single layer of the second polyester fiber web is independently 0.3-0.35 mm.

[0039] According to the present invention, preferably, when the number of layers of the second polyester fiber web is 3, the thickness of each single layer of the second polyester fiber web is independently 0.2-0.25 mm.

[0040] According to the present invention, preferably, the ratio of the thickness of a single layer of the first polyester fiber web to the thickness of a single layer of the second polyester fiber web is 0.25-1:1 (for example, 0.25:1, 0.35:1, 0.45:1, 0.55:1, 0.65:1, 0.75:1, 0.85:1, 0.95:1, 1:1, and any two of the above ranges), more preferably 0.5-0.75:1.

[0041] In this invention, it is understood that the alternating laying of the first polyester fiber and the second polyester fiber is as follows: first, the first polyester fiber is laid to obtain a first layer of polyester fiber web; then, the second polyester fiber is laid on the first layer of polyester fiber web to obtain a second layer of polyester fiber web; then, the first polyester fiber is laid on the second layer of polyester fiber web to obtain a third layer of polyester fiber web, and so on. By alternating the laying of the first polyester fiber and the second polyester fiber in this manner, a multi-layered polyester fiber web is obtained.

[0042] The materials of the first and second polyester fibers described in the second aspect are the same as those described in the first aspect, and will not be repeated here.

[0043] According to the present invention, preferably, the first polyester fiber and the second polyester fiber each independently satisfy the following: average diameter of 15-35 μm and diameter standard deviation ≤2 μm.

[0044] In this invention, the average diameter of the first polyester fiber and the second polyester fiber can each independently be 15μm, 18μm, 20μm, 22μm, 24μm, 26μm, 28μm, 30μm, 32μm, 35μm, or any range formed by any two of the above points. The standard deviation of the diameter of the first polyester fiber and the second polyester fiber can each independently be 2μm, 1.8μm, 1.6μm, 1.4μm, 1.2μm, 1μm, 0.8μm, 0.6μm, 0.4μm, 0.2μm, 0.1μm, or any range formed by any two of the above points, for example, 0.1-2μm.

[0045] In this invention, the method for testing the standard deviation of diameter is as follows: Take 50 composite polyester fibers, randomly cut a small section from each fiber, take a scanning electron microscope (SEM) image, and measure the diameter of each fiber from the image to an accuracy of 0.1 μm. Then, calculate the standard deviation of these diameter values ​​according to the standard deviation calculation method.

[0046] In this invention, the first polyester fiber and the second polyester fiber refer to polyester fibers with a length greater than 150 mm.

[0047] According to the present invention, preferably, the hot pressing temperature is 180-210°C.

[0048] According to the present invention, preferably, the method for preparing the first polyester fiber includes: melting the first polyester to obtain a melt, then extruding the melt from a spinneret with a circular spinneret nozzle to obtain a filamentous melt; then cooling the filamentous melt into a filamentous solid using side blowing air, then dividing the filamentous solid into one or more groups of fiber bundles, and drawing each group of fiber bundles into a drawing tube with a high-speed airflow inside, and stretching it using the high-speed airflow to obtain the first polyester fiber. The second polyester fiber is also prepared by the above method.

[0049] According to the present invention, preferably, the melting temperature is 260-280°C when preparing the first polyester fiber.

[0050] According to the present invention, preferably, when preparing the first polyester fiber, the wind speed of the side blowing air for cooling the filamentous melt is 4-6 m / s, and the flow rate of the high-speed airflow for stretching the filamentous solid is 4000-4200 m / min.

[0051] According to the present invention, preferably, the melting temperature is 230-255°C when preparing the second polyester fiber.

[0052] According to the present invention, preferably, when preparing the second polyester fiber, the wind speed of the side blowing air for cooling the filamentous melt is 4-6 m / s, and the flow rate of the high-speed airflow for stretching the filamentous solid is 4100-4300 m / min.

[0053] According to the present invention, preferably, the inner diameter of the circular spinneret in the spinneret plate having a circular spinneret is 15-25 μm, and the difference between the maximum inner diameter and the minimum inner diameter is ≤2 μm.

[0054] According to the present invention, preferably, the inner diameter of the circular tube is 2-3 cm, the distance between two adjacent circular tubes is 3.5-4 cm, and the length of the circular tube is 1.5-2 m. The distance between two adjacent circular tubes refers to the distance between the centers of two adjacent circular tubes. The length of the circular tube refers to the distance along the fiber ejection direction.

[0055] The third aspect of the present invention provides a composite polyester fiber fabric prepared by the method described above.

[0056] The fourth aspect of the present invention provides the application of the composite polyester fiber cloth described above and / or the composite polyester fiber cloth prepared by the method described above in cell culture.

[0057] According to the present invention, preferably, the application includes increasing the rate of cellular glucose metabolism. Preferably, cell culture using the fiber cloth of the present invention can achieve a cellular glucose metabolism rate of 20 g / day or higher, for example, 20-35 g / day.

[0058] The present invention will be described in detail below through embodiments. In the following embodiments,

[0059] The inner diameter of the circular spinneret in the spinneret is 25 μm, and the difference between the maximum and minimum inner diameter is 1 μm.

[0060] The method for testing the areal density of composite polyester fiber fabric is as follows: Use a 1 / 10,000 balance to measure the areal density of 1 cm⁻¹. 2 The area of ​​the fiber cloth is weighed, and then the mass of the fiber cloth is divided by its area; the thickness of the composite polyester fiber cloth is tested using a dial thickness gauge (model 547-313) from Suzhou Quantum Instruments Co., Ltd.

[0061] The Sb content in the first and second polyesters is ≤80ppm.

[0062] In the following examples, the self-made polyester involved is prepared by mixing PTA (terephthalic acid, analytical grade), EG (ethylene glycol, analytical grade) and optional comonomer Y (e.g., cyclohexanediol, analytical grade) in a reaction vessel according to the required amount of product, heating to 258-263°C, reacting for about 1 hour, then adding tetrabutyl titanate, raising the temperature to 275-290°C, and using an oil pump to evacuate the vacuum, reacting for about 2 hours to obtain the desired PET.

[0063] Example 1

[0064] (1) The first polyester and the second polyester are melted at 280°C and 250°C respectively to obtain melts. The melts are then extruded from spinnerets with circular spinnerets to obtain first filamentous melts and second filamentous melts. The first filamentous melts and the second filamentous melts are cooled into first filamentous solids and second filamentous solids respectively by side blowing. The first filamentous solids and the second filamentous solids are then divided into one or more groups of fiber bundles. Each group of fiber bundles is drawn into a drawing tube with a high-speed airflow passing through it. The high-speed airflow is used to stretch the fiber to obtain the first polyester fiber and the second polyester fiber. The first polyester is homopolymer polyethylene terephthalate (manufacturer: Yizheng Chemical Fiber Co., Ltd. of Sinopec, hereinafter referred to as "Yizheng Chemical Fiber", model FG600, intrinsic viscosity 0.675 dL / g, standard deviation of intrinsic viscosity 0.003 dL / g, melting point 261℃); the second polyester is copolymer polyethylene terephthalate (Yizheng Chemical Fiber, model FG702, intrinsic viscosity 0.78 dL / g, standard deviation of intrinsic viscosity 0.006 dL / g, comonomer is cyclohexanediol, comonomer content is 15 mol%, melting point 140℃). The side-blowing air velocity for cooling the first filamentous melt is 5 m / s, the side-blowing air velocity for cooling the second filamentous melt is 4 m / s, the high-speed airflow velocity for stretching the first filamentous solid is 4000 m / min, and the high-speed airflow velocity for stretching the second filamentous solid is 4300 m / min. The inner diameter of the circular tube is 2 cm, the distance between two adjacent circular tubes is 3.5 cm, and the length of the circular tube is 2 m. The average diameter of the first and second polyester fibers and the standard deviation of the diameter were measured. The test results are shown in Table 1.

[0065] (2) The first polyester fiber and the second polyester fiber are laid in layers in the order of first polyester fiber (first layer), second polyester fiber (second layer), and first polyester fiber (third layer) to form a (three-layer) fiber web with a sandwich structure; wherein the thickness of the first layer is 0.3 mm, the thickness of the second layer is 0.4 mm, and the thickness of the third layer is 0.3 mm. Then, the fiber web is hot-pressed at 190-200℃ using a polished hot roller without embossing to obtain a composite polyester fiber fabric. The areal density and thickness of the composite polyester fiber fabric are shown in Table 1.

[0066] Example 2

[0067] (1) The first polyester and the second polyester are melted at 280°C and 270°C respectively to obtain melts. The melts are then extruded from spinnerets with circular spinnerets to obtain first filamentous melts and second filamentous melts. The first filamentous melts and the second filamentous melts are cooled into first filamentous solids and second filamentous solids respectively by side blowing. The first filamentous solids and the second filamentous solids are then divided into one or more groups of fiber bundles. Each group of fiber bundles is drawn into a drawing tube with a high-speed airflow passing through it. The high-speed airflow is used to stretch the fiber to obtain the first polyester fiber and the second polyester fiber. The first polyester is homopolymer polyethylene terephthalate (manufacturer: Yizheng Chemical Fiber Co., Ltd. of Sinopec, hereinafter referred to as "Yizheng Chemical Fiber", model FG600, intrinsic viscosity 0.675 dL / g, standard deviation of intrinsic viscosity 0.003 dL / g, melting point 261℃); the second polyester is copolymer polyethylene terephthalate (Yizheng Chemical Fiber, model FG702, intrinsic viscosity 0.78 dL / g, standard deviation of intrinsic viscosity 0.006 dL / g, comonomer is cyclohexanediol, comonomer content is 15 mol%, melting point 140℃). The side-blowing air velocity for cooling the first filamentous melt is 5 m / s, the side-blowing air velocity for cooling the second filamentous melt is 5 m / s, the high-speed airflow velocity for stretching the first filamentous solid is 4200 m / min, and the high-speed airflow velocity for stretching the second filamentous solid is 4100 m / min. The inner diameter of the circular tube is 2 cm, the distance between two adjacent circular tubes is 3.5 cm, and the length of the circular tube is 2 m. The average diameter of the first and second polyester fibers and the standard deviation of the diameter were measured. The test results are shown in Table 1.

[0068] (2) The first polyester fiber and the second polyester fiber are laid in layers in the order of first polyester fiber (first layer), second polyester fiber (second layer), and first polyester fiber (third layer) to form a (three-layer) fiber web with a sandwich structure; wherein the thickness of the first layer is 0.25 mm, the thickness of the second layer is 0.5 mm, and the thickness of the third layer is 0.25 mm. Then, the fiber web is hot-pressed at 190-200℃ using a polished hot roller without embossing to obtain a composite polyester fiber fabric. The areal density and thickness of the composite polyester fiber fabric are shown in Table 1.

[0069] Example 3

[0070] The method was carried out according to Example 1, except that the first polyester was homopolymer polyethylene terephthalate (self-made polyester, without added comonomer Y, intrinsic viscosity 0.682 dL / g, standard deviation of intrinsic viscosity 0.005 dL / g, melting point 260℃); the second polyester was copolymer polyethylene terephthalate (Yizheng Chemical Fiber, model BG804, intrinsic viscosity 0.801 dL / g, standard deviation of intrinsic viscosity 0.005 dL / g, comonomer was cyclohexanediol, comonomer content was 0.75 mol%, melting point 237℃).

[0071] Example 4

[0072] The method is carried out according to Example 1, except that the first polyester fiber and the second polyester fiber are laid in layers in the following order: first polyester fiber (first layer), second polyester fiber (second layer), first polyester fiber (third layer), second polyester fiber (fourth layer), and first polyester fiber (fifth layer) to form a five-layer fiber web with a sandwich structure. The thickness of the first layer is 0.15 mm, the thickness of the second layer is 0.3 mm, the thickness of the third layer is 0.15 mm, the thickness of the fourth layer is 0.3 mm, and the thickness of the fifth layer is 0.15 mm.

[0073] Example 5

[0074] The method is carried out according to Example 1, except that the first polyester fiber and the second polyester fiber are laid in layers in the following order: first polyester fiber (first layer), second polyester fiber (second layer), first polyester fiber (third layer), second polyester fiber (fourth layer), first polyester fiber (fifth layer), second polyester fiber (sixth layer), and first polyester fiber (seventh layer) to form a (seven-layer) fiber web with a sandwich structure. The thickness of the first layer is 0.1 mm, the thickness of the second layer is 0.2 mm, the thickness of the third layer is 0.1 mm, the thickness of the fourth layer is 0.2 mm, the thickness of the fifth layer is 0.1 mm, the thickness of the sixth layer is 0.2 mm, and the thickness of the seventh layer is 0.1 mm.

[0075] Example 6

[0076] The method is carried out according to Example 1, except that the thickness of the first layer is 0.3 mm, the thickness of the second layer is 0.1 mm, and the thickness of the third layer is 0.3 mm.

[0077] Comparative Example 1

[0078] The method was carried out according to Example 1, except that the first polyester was homopolymer polyethylene terephthalate (manufacturer: Sinopec Yizheng Chemical Fiber Co., Ltd., model BG85, intrinsic viscosity 0.879 dL / g, standard deviation of intrinsic viscosity 0.005 dL / g, melting point 248℃); the second polyester was copolymer polyethylene terephthalate (Yizheng Chemical Fiber, model BG804, intrinsic viscosity 0.801 dL / g, standard deviation of intrinsic viscosity 0.005 dL / g, comonomer: cyclohexanediol, comonomer content 0.75 mol%, melting point 237℃).

[0079] Comparative Example 2

[0080] The method is carried out according to Example 1, except that the first polyester fiber and the second polyester fiber are laid in layers in the order of second polyester fiber (first layer), first polyester fiber (second layer), and second polyester fiber (third layer) to form a (three-layer) fiber web with a sandwich structure, wherein the thickness of the first layer is 0.3 mm, the thickness of the second layer is 0.4 mm, and the thickness of the third layer is 0.3 mm.

[0081] Test Example 1

[0082] Pretreatment and sterilization of the fiber cloth: The fiber cloth was soaked in a 7% (w / w) hydrogen peroxide aqueous solution at room temperature for 60 minutes; then, it was ultrasonically cleaned with ultrapure water and dried at 80°C. The dried fiber cloth was then sterilized by high-temperature steam at 121°C for 60 minutes.

[0083] Then, the glucose metabolism capacity of Vero cells was tested using sterilized fiber cloth as a carrier. The glucose metabolism test method was as follows: Fiber cloth was cut into 6mm × 6mm pieces. 100g of the 6mm × 6mm fiber cloth, 8L of PBS buffer, and approximately 200mL of 199 culture medium (purchased from Beijing Tsinghua Tianyi Biotechnology Co., Ltd.) were placed in a 10L basket reactor. 10% of superior newborn calf serum (purchased from Lanzhou Minhai Biotechnology Co., Ltd.) relative to the culture medium was added, along with an appropriate amount of 20% by weight glucose to bring the total glucose concentration to 0.5% by weight. Vero cells (purchased from the National Biomedical Experimental Cell Resource Bank, fourth-generation Vero cells, per 1cm²) were then seeded. 2 The inoculation density of cells on the fiber cloth was 0.5 × 10⁻⁶. 6Individual samples were cultured at 37℃ with aeration. During cultivation, the pH was set to 7.3, DO (dissolved oxygen as a percentage of saturated dissolved oxygen) to 60%, and the carbon dioxide concentration to 5%, with slow stirring for 7 days. Samples were taken every 24 hours, and glucose content was measured using a glucose assay kit (purchased from Nanjing Jiancheng Bioengineering Institute). The glucose concentration was then adjusted to 0.5% by weight using 20% ​​glucose solution. The decrease in glucose content was converted to a glucose consumption rate value, expressed in g / day. A curve was plotted on the glucose consumption rate against cultivation time, and the value at 100 hours was taken as the 100-hour glucose metabolism rate value. The 100-hour glucose metabolism rate values ​​are shown in Table 1.

[0084] Table 1

[0085]

[0086] The preferred embodiments of the present invention have been described in detail above; however, the present invention is not limited thereto. Within the scope of the inventive concept, various simple modifications can be made to the technical solutions of the present invention, including combinations of various technical features in any other suitable manner. These simple modifications and combinations should also be considered as the content disclosed in the present invention and are all within the protection scope of the present invention.

Claims

1. A composite polyester fiber fabric, characterized in that, The composite polyester fiber fabric includes a first polyester fiber layer and a second polyester fiber layer, and the first polyester fiber layer and the second polyester fiber layer are alternately stacked. The number of layers of the first polyester fiber layer is n, the number of layers of the second polyester fiber layer is n-1, and n≥2. The melting point of the first polyester fiber in the first polyester fiber layer is more than 20°C higher than the melting point of the second polyester fiber in the second polyester fiber layer. The material of the first polyester fiber is homopolymer polyethylene terephthalate, and the material of the second polyester fiber is copolymer polyethylene terephthalate; the intrinsic viscosity of the first polyester fiber and the second polyester fiber are each independently 0.6-0.85 dL / g, and the standard deviation of the intrinsic viscosity is each independently ≤0.05 dL / g.

2. The composite polyester fiber fabric according to claim 1, wherein, n is any integer from 2 to 10; And / or, the composite polyester fiber fabric has a thickness of 0.4-0.5 mm and an areal density of 80-130 g / m³. 2 .

3. The composite polyester fiber fabric according to claim 1, wherein, The Sb content in the materials of the first and second polyester fibers is ≤80ppm each independently.

4. The composite polyester fiber fabric according to claim 1, wherein, The first and second polyester fibers each independently satisfy the following: average diameter of 15-35 μm and diameter standard deviation ≤2 μm.

5. A method for preparing composite polyester fiber fabric, characterized in that, The method includes: alternately laying a first polyester fiber and a second polyester fiber into a web, and then hot-pressing and bonding them; wherein the number of layers of the first polyester fiber web is n, the number of layers of the second polyester fiber web is n-1, n≥2, and the melting point of the first polyester fiber is more than 20°C higher than the melting point of the second polyester fiber. The material of the first polyester fiber is homopolymer polyethylene terephthalate, and the material of the second polyester fiber is copolymer polyethylene terephthalate; the intrinsic viscosity of the first polyester fiber and the second polyester fiber are each independently 0.6-0.85 dL / g, and the standard deviation of the intrinsic viscosity is each independently ≤0.05 dL / g.

6. The method according to claim 5, wherein, n is any integer from 2 to 10; And / or, the thickness of each single layer of the first polyester fiber web is independently 0.08-0.35 mm; And / or, the thickness of each single layer of the second polyester fiber web is independently 0.08-0.6 mm; And / or, the ratio of the thickness of a single layer of the first polyester fiber web to the thickness of a single layer of the second polyester fiber web is 0.25-1:1; And / or, the Sb content in the materials of the first polyester fiber and the second polyester fiber is independently ≤80ppm.

7. The method according to claim 5, wherein, The thickness of each single layer of the first polyester fiber web is independently 0.1-0.3 mm.

8. The method according to claim 5, wherein, The thickness of each single layer of the second polyester fiber web is independently 0.2-0.5 mm.

9. The method according to claim 5, wherein, The ratio of the thickness of a single layer of the first polyester fiber web to the thickness of a single layer of the second polyester fiber web is 0.5-0.75:

1.

10. The method according to claim 5, wherein, The first and second polyester fibers each independently satisfy the following: average diameter of 15-35 μm and diameter standard deviation ≤2 μm.

11. The method according to claim 5, wherein, The hot pressing temperature is 180-210℃.

12. The composite polyester fiber fabric prepared by the method according to any one of claims 5-11.

13. The application of the composite polyester fiber fabric according to any one of claims 1-4 and 12 or the composite polyester fiber fabric prepared by the method according to any one of claims 5-11 in cell culture.

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