A light-shielding enzyme labeling plate with a closed bottom

Abstract

The present disclosure relates to the technical field of ELISA plates. An embodiment of the present disclosure provides a light-shielding ELISA plate with a closed bottom, which includes a base plate and a detection tube, a plurality of ELISA holes are provided on the base plate, and the detection tube is located inside the ELISA hole. The plate also includes a dripping bucket, a baffle, a partition and a capping structure. The dripping bucket is fixedly connected to the top of the detection tube, a retaining groove matching the baffle is provided on the base plate, and the baffle is slidably installed inside the retaining groove. The partition and the baffle are fixedly connected, and the partition and the base plate are in a sliding fit. The capping structure is used to cap the tops of multiple detection tubes on the same base plate and to keep the detection tube that needs to drop a sample in an open state. The capping structure includes a shielding frame and a dripping frame. The shielding frame is detachably connected to the base plate, and the dripping frame is slidably installed on the shielding frame. Through the above technical solution, the technical problem in the prior art that the sample is easily splashed into other ELISA holes when dripping, thereby reducing the detection accuracy, is solved.

Description

Technical Field

[0001] The embodiments of the present disclosure relate to the technical field of ELISA plates, and in particular, to a light-shielding ELISA plate with a closed bottom. Background Art

[0002] The main material of the ELISA plate is polystyrene (PS), which is an indispensable experimental tool for enzyme-linked immunosorbent assay (ELISA). As a solid phase carrier, the ELISA plate mainly adsorbs antigens, antibodies and other biological molecules into the plate wells through passive adsorption of hydrophobic bonds and ionic bonds, or through covalent bonding by introducing other active groups such as amino and carbon groups, or through hydrophilic bond binding after surface modification. The grid on the traditional ELISA plate is of the penetrating type, and the cup body of the microporous cup used with it is transparent. Using the above method, light pollution is more serious and the test results are not accurate enough. In addition, the microporous cup used in conjunction with the ELISA plate can only be a special non-light-transmitting microporous cup, and the production cost is higher than the traditional transparent microporous cup in terms of process cost.

[0003] Therefore, a light-shielding ELISA plate with a closed bottom was developed, which adopts the traditional non-transparent ELISA plate body, but the bottom of the grid groove on the ELISA plate body is closed and non-transparent. After penetrating the enzyme solution, the detection light is reflected back to the upper part of the light-shielding ELISA plate, which is convenient for the optical detection equipment to perform detection on the upper part of the light-shielding ELISA plate. In this process, light pollution is reduced and the detection accuracy is improved. At the same time, the microwell cup used in conjunction with the light-shielding ELISA plate can use a traditional transparent well cup for luminescence detection, and does not require a special light-shielding ELISA well cup, which saves costs. However, since the ELISA wells of the current ELISA plate are mostly arranged vertically and the ELISA wells are not blocked and protected, it is easy for the sample to splash into the interior of the surrounding ELISA wells when the sample is dripped in. In the process of moving the sample to the corresponding ELISA well, it may also drip into the interior of other ELISA wells due to hand shaking or misoperation, resulting in inaccurate test results. This risk is more likely to occur when dripping into the ELISA well in the middle position. Summary of the Invention

[0004] To overcome the above-mentioned defects, the embodiments of the present disclosure provide a light-shielding ELISA plate with a closed bottom, which solves the technical problem in the prior art that when the sample is dripped into the plate, the sample easily splashes into other ELISA wells, thereby reducing the detection accuracy.

[0005] According to one aspect, at least one embodiment of the present disclosure provides a light-shielding ELISA plate with a closed bottom, comprising a base plate and a detection cylinder, wherein the base plates are provided in plurality and are detachably connected to each other, the base plate is provided with a plurality of ELISA wells, the detection cylinders correspond one to one with the ELISA wells, the detection cylinders are located inside the ELISA wells, and the plurality of detection cylinders in the same row are fixedly connected by a connecting block, further comprising:

[0006] A dripping hopper, the dripping hopper being fixedly connected to the top end of the detection cylinder;

[0007] baffles, wherein a plurality of baffles are provided, a baffle groove matching the baffle is opened on the bottom plate, the baffle is slidably mounted inside the baffle groove, and the baffle groove is located between two adjacent enzyme labeling holes;

[0008] A partition, wherein the partition is fixedly connected to the baffle and is in sliding fit with the bottom plate;

[0009] The sealing structure is used to seal the tops of multiple detection cylinders on the same base plate and keep the detection cylinders that need to be dripped with samples in an open state. The sealing structure includes:

[0010] a shielding frame, the shielding frame being detachably connected to the base plate;

[0011] The dripping frame is slidably mounted on the shielding frame, both ends of the dripping frame are fixedly connected with shielding pieces, and one end of the shielding piece away from the dripping frame is fixedly connected to the inner wall of the shielding frame.

[0012] In order to realize the detachable connection between the base plates, a plurality of sliders are fixedly connected to one end of the base plate, and a plurality of sliding grooves matching the sliders are opened at the other end of the base plate.

[0013] In order to realize the detachable connection between the shielding frame and the bottom plate, the shielding frame is detachably connected to the bottom plate through a connecting piece, and the connecting piece includes:

[0014] An insertion rod, the insertion rod is fixedly connected to the bottom end of the shielding frame, and the bottom plate is provided with a slot matching the insertion rod;

[0015] A card block, wherein a fixing groove is provided on the bottom plate, the fixing groove is communicated with the slot, the card block is connected to the inside of the fixing groove via a compression spring, and a card slot matching the card block is provided on the insertion rod;

[0016] A pull rod is fixedly connected to the clamping block, the pull rod and the base plate are in sliding fit, and the pull rod passes through the base plate and extends to the outside.

[0017] In order to reduce the obstruction of the baffle and the partition to the shielding frame, a supporting spring is connected between the bottom end of the baffle and the inner bottom wall of the retaining groove.

[0018] In order to reduce the obstruction of the baffle to the detection cylinder and the connecting block during installation, a through opening matching the connecting block is opened on the baffle.

[0019] In order to prevent the sample from splashing into other test bottles through the through opening, a plurality of elastic protective sheets are fixedly connected to both side walls of the through opening, and the plurality of elastic protective sheets seal the through opening.

[0020] In order to make the position of the dripping frame correspond to the position of the dripping bucket, a plurality of positioning grooves are provided on the shielding frame, and the positions of the positioning grooves correspond one-to-one to the positions of the enzyme-labeled holes. The interior of the positioning groove is connected to a positioning block through a positioning spring, and the dripping frame is provided with a positioning hole that matches the positioning block.

[0021] In order to reduce the residual sample on the dripping frame and the shielding piece from entering the other detection cylinders, the top of the baffle is detachably connected to a sponge pad, and the second sponge pad contacts the bottom of the dripping frame and the shielding piece.

[0022] In order to achieve detachable connection between the sponge pad and the baffle, the sponge pad is detachably connected to the top end of the baffle via Velcro.

[0023] The beneficial effects of the embodiments of the present disclosure are:

[0024] 1. In the present disclosure, the range of dripping samples or reagents is expanded by the dripping funnel, which facilitates accurate dripping into the interior of the detection cylinder and reduces splashing of samples or reagents.

[0025] 2. In the present disclosure, adjacent test bottles are separated and shielded to a certain extent by baffles and partitions. Under the action of the support spring, the height of the baffles and partitions is higher than the dripping funnel, thereby reducing the impact of splashing of samples and reagents on the surrounding test cylinders.

[0026] 3. In the present disclosure, the shielding frame is installed on the top of the base plate by cooperating with the insertion rod and the card block. The shielding piece blocks the top of the dripping bucket. By moving the dripping frame, the top of the corresponding detection cylinder and the dripping bucket are opened, and samples or reagents can be dripped into the corresponding detection cylinder.

[0027] 4. In the present disclosure, the provision of the through-port avoids obstruction to the removal and placement of the detection tube and the connecting block. At the same time, after removal and placement, the elastic protective sheet returns to its original position under its own elastic action, closes the through-port, ensures the shielding effect of the baffle, and reduces the impact of splashes on other samples.

[0028] 5. Therefore, compared with the ELISA plate in the prior art, the present invention expands the dripping range of the detection tube through the dripping bucket, while reducing the splashing of samples and reagents, and shields the dripping bucket through the baffle and partition to further reduce the impact of splashing on other detection tubes. The shielding frame is installed above multiple detection tubes, and the dripping bucket and the top opening of the detection tube are shielded by the shielding sheet. By moving the dripping frame to the top of the corresponding dripping bucket, the sample can be dripped into the interior of the detection tube, and the shielding sheet shields other dripping buckets to prevent the dripping material from splashing into the interior of other detection tubes and affecting the detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] To more clearly illustrate the technical solutions in the embodiments of the present disclosure, the following briefly describes the drawings required for use in describing the embodiments of the present disclosure. Obviously, the drawings described below are merely some exemplary embodiments of the present disclosure. Those skilled in the art can, without inventive effort, derive other drawings based on the content of the exemplary embodiments of the present disclosure and these drawings.

[0030] Figure 1 This is a schematic structural diagram of a first perspective in an embodiment of the present disclosure;

[0031] Figure 2 for Figure 1 A schematic structural diagram of a second viewing angle in an embodiment of the present invention;

[0032] Figure 3 for Figure 1 A schematic structural diagram of the detection tube, the connecting block and the dripping bucket in the embodiment;

[0033] Figure 4 for Figure 1 A schematic structural diagram of the bottom plate, the detection cylinder, the baffle and the partition in the embodiment of FIG;

[0034] Figure 5 for Figure 1 A schematic structural diagram of the bottom plate, the detection cylinder and the sealing cover structure in the embodiment;

[0035] Figure 6 for Figure 1 A schematic structural diagram of the cover structure in an embodiment of the present invention;

[0036] Figure 7 for Figure 1 A schematic structural diagram of the bottom plate, baffle, partition and sponge pad in the embodiment of FIG;

[0037] Figure 8 for Figure 7 Schematic diagram of the local enlarged structure at D in the middle;

[0038] Figure 9 for Figure 4 Schematic diagram of the local enlarged structure at B in the middle;

[0039] Figure 10 for Figure 6 Schematic diagram of the local enlarged structure at C in the middle;

[0040] Figure 11 for Figure 1 Schematic diagram of the locally enlarged structure at point A in the middle.

[0041] In the picture:

[0042] 1. Bottom plate; 2. Detection tube; 3. Connecting block; 4. Slider; 5. Drip hopper; 6. Baffle; 7. Partition; 8. Support spring; 9. Elastic protective sheet; 10. Positioning spring; 11. Positioning block; 12. Sponge pad; 13. Identification plate;

[0043] 101. shielding frame; 102. dripping frame; 103. shielding sheet;

[0044] 201. Insert rod; 202. Block; 203. Pull rod; 204. Compression spring. DETAILED DESCRIPTION

[0045] The present disclosure will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present disclosure, rather than to limit the present disclosure.

[0046] To simplify the drawings, only the parts relevant to the disclosure are schematically shown in each figure; they do not represent the actual structure of the product. Furthermore, to simplify the drawings and facilitate understanding, in some figures, only one of the components with the same structure or function is schematically shown or labeled. In this document, "one" not only means "only one" but also "more than one," and "several" includes "two" and "more than two."

[0047] It should be noted that, unless otherwise specified or limited, the terms "mounted," "connected," and "connected" should be understood broadly. For example, they can refer to fixed, detachable, or integral connections; mechanical or electrical connections; direct or indirect connections through an intermediary; and internal communication between two components. Those skilled in the art will understand the specific meanings of these terms in this disclosure based on the specific circumstances.

[0048] In the present disclosure, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Moreover, a first feature being "above," "above," and "above" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature includes the first feature being directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0049] In the description of this embodiment, the terms "up", "down", "left", "right", etc., and the orientation or position relationship are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they should not be understood as limitations on the present disclosure.

[0050] In addition, in the description of the present application, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0051] like Figures 1 to 11 As shown, it shows a bottom-closed light-shielding enzyme labeling plate in an embodiment of the present disclosure, including a bottom plate 1 and a detection tube 2. The bottom plate 1 is a closed and light-proof structure. The detection light is reflected back to the upper part of the light-shielding enzyme labeling plate after penetrating the enzyme liquid, which is convenient for the optical detection equipment to perform detection on the upper part of the light-shielding enzyme labeling plate. In this process, light pollution is reduced and the detection accuracy is improved. The bottom plate 1 is provided with multiple, and the multiple bottom plates 1 are detachably connected. To achieve the detachable connection between the bottom plates 1, one end of the bottom plate 1 is fixedly connected to multiple sliders 4, and the other end of the bottom plate 1 is provided with multiple sliding grooves matching the sliders 4. Through the cooperation of the sliders 4 and the sliding grooves, the two bottom plates 1 can be connected. By changing the number of bottom plates 1, it can adapt to different detection requirements and the number of detection samples can be adjusted more freely. There is a certain sliding damping between the sliders 4 and the sliding grooves, which can ensure the stability of the connection between the bottom plates 1. A plurality of enzyme-labeled wells are provided on the plate 1, and the detection cylinders 2 correspond to the enzyme-labeled wells one by one. The detection cylinders 2 are located inside the enzyme-labeled wells. The multiple detection cylinders 2 in the same row are fixedly connected by connecting blocks 3. The plate 1 also includes a dripping bucket 5, a baffle 6, a partition 7 and a sealing structure. Compared with the enzyme-labeled plate in the prior art, the present invention expands the dripping range of the detection cylinder 2 by the dripping bucket 5, while reducing the splashing of samples and reagents. The dripping bucket 5 is shielded by the baffle 6 and the partition 7 to further reduce the impact of the splashing on other detection cylinders 2. The shielding frame 101 is installed above the multiple detection cylinders 2, and the dripping bucket 5 and the top opening of the detection cylinder 2 are shielded by the shielding sheet 103. By moving the dripping frame 102 above the corresponding dripping bucket 5, the detection cylinder 2 can be dripped into the interior of the detection cylinder 2. At the same time, the shielding sheet 103 shields other dripping buckets 5 to prevent the dripping material from splashing into the interior of other detection cylinders 2 and affecting the detection results.

[0052] The dropping funnel 5 is fixedly connected to the top of the detection cylinder 2. The dropping funnel 5 is larger at the top and smaller at the bottom, which increases the dropping range, reduces the operator's misoperation when dropping the sample, and makes it easier to identify and judge the range of the detection cylinder 2. The outlet of the burette is better moved above the detection cylinder 2. At the same time, the shape of larger at the top and smaller at the bottom reduces splashing during dropping. Even if droplets splash, the droplets are caught by the presence of the dropping funnel 5. At the same time, the droplets flow back into the interior of the detection cylinder 2 along the dropping funnel 5, thereby ensuring the accuracy of the test results.

[0053] The baffles 6 are provided with a plurality of baffles, and a baffle groove matching the baffle 6 is provided on the bottom plate 1. The baffle 6 is slidably installed in the inside of the baffle groove. The baffle groove is located between two adjacent enzyme-labeled holes. A support spring 8 is connected between the bottom end of the baffle 6 and the inner bottom wall of the baffle groove. In order to reduce the obstruction of the baffle 6 to the installation of the detection cylinder 2 and the connecting block 3, a through-hole matching the connecting block 3 is provided on the baffle 6. At the same time, in order to prevent the sample from splashing into other detection bottles through the through-hole, multiple elastic protective sheets 9 are fixedly connected to the two side walls of the through-hole. The multiple elastic protective sheets 9 close the through-hole. The partition 7 is fixedly connected to the baffle 6, and the partition 7 and the bottom plate 1 are in sliding cooperation. The support spring 8 supports the baffle 6 and the partition 7, so that the baffle 6 and the partition 7 are in a higher position, separating the adjacent drop-in buckets 5. A base plate 1 corresponds to the same row of detection cylinders 2, and two adjacent detection cylinders 2 in the same row are fixedly connected by a connecting block 3. When the detection cylinder 2 is placed into the enzyme-labeled hole in the base plate 1 or the detection cylinder 2 is taken out of the enzyme-labeled hole, the opening is opened to avoid obstruction of the connecting block 3. The existence of the opening will increase the risk of liquid splashing to other detection cylinders 2. The opening can be closed by the elastic protective sheet 9. At the same time, when the connecting block 3 moves, the elastic bending of the elastic protective sheet 9 can avoid obstruction to the movement of the connecting block 3. After the detection cylinder 2 is installed or taken out, the elastic protective sheet 9 returns to its original state, that is, the horizontal state, under the action of its own elasticity. Multiple elastic protective sheets 9 are tightly arranged to protect the opening to prevent liquid from splashing to other locations through the opening.

[0054] The covering structure is used to cover the tops of multiple detection cylinders 2 on the same base plate 1, and to keep the detection cylinder 2 that needs to drip the sample in an open state. The covering structure includes a shielding frame 101 and a dripping frame 102. The shielding frame 101 is detachably connected to the base plate 1, and the dripping frame 102 is slidably installed on the shielding frame 101. Both ends of the dripping frame 102 are fixedly connected with shielding pieces 103. The end of the shielding piece 103 away from the dripping frame 102 is fixedly connected to the inner wall of the shielding frame 101. The shielding frame 101 is installed on the top of the base plate 1 and is located above the dripping bucket 5. During the installation of the shielding frame 101, the baffle 6 and the partition 7 will be pressed to make the top of the baffle 6 and the partition 7 drop to be flush with the top of the dripping bucket 5, and the baffle 6 and the partition are adapted to the compression of the support spring 8. 7 descends, the shielding piece 103 is folded and can be moved with the protective frame. The size of the protective frame and the dripping bucket 5 are adapted. The protective frame moves to the top of the corresponding dripping bucket 5, and the sample or reagent can be added to the detection cylinder 2 corresponding to the dripping bucket 5. At this time, the shielding piece 103 shields other dripping buckets 5 to prevent liquid from accidentally falling or splashing into the interior of other detection cylinders 2 during dripping, thereby ensuring the accuracy of the test results. The length of the shielding frame 101 is longer than the length of the base plate 1 by at least the length of the dripping frame 102. After all the detection cylinders 2 on one base plate 1 are added with samples or reagents, the dripping frame 102 can be moved to the edge of the shielding frame 101. At this time, the dripping frame 102 is away from multiple dripping buckets 5, and the shielding piece 103 seals the tops of all dripping buckets 5.

[0055] In order to realize the detachable connection between the shielding frame 101 and the base plate 1, the shielding frame 101 is detachably connected to the base plate 1 through a connecting piece, and the connecting piece includes an insert rod 201, a block 202 and a pull rod 203. The insert rod 201 is fixedly connected to the bottom end of the shielding frame 101, and a slot matching the insert rod 201 is provided on the base plate 1. A fixing slot is provided on the base plate 1, and the fixing slot and the slot are connected. The block 202 is connected to the inside of the fixing slot through a compression spring 204. A slot matching the block 202 is provided on the insert rod 201. The pull rod 203 is fixedly connected to the block 202. The pull rod 203 and the base plate 1 are in a sliding fit. The pull rod 203 passes through the base plate 1 and extends to the outside, and the insert rod 201 is inserted into the slot. The block 202 is wedge-shaped. As the insertion rod 201 and the block 202 contact and descend, the block 202 can be pushed into the interior of the fixed groove to compress the compression spring 204. When the insertion rod 201 descends to a suitable position, the shielding frame 101 contacts the top of the dripping bucket 5, and at the same time, the slot just descends to correspond to the position of the block 202. Under the action of the compression spring 204, the block 202 is stuck in the interior of the slot, and the block 202 limits the insertion rod 201 to ensure the stability of the shielding frame 101. When the shielding frame 101 needs to be disassembled, the pull rod 203 is pulled out of the interior of the slot, and the insertion rod 201 can be moved out from the inside of the slot to disassemble the shielding frame 101.

[0056] In order to make the position of the dripping frame 102 correspond to the position of the dripping bucket 5, a plurality of positioning grooves are provided on the shielding frame 101. The positions of the positioning grooves correspond to the positions of the enzyme-labeled holes one by one. The interior of the positioning grooves is connected to a positioning block 11 through a positioning spring 10. A positioning hole matching the positioning block 11 is provided on the dripping frame 102. The positioning block 11 is spherical. As the dripping frame 102 moves, the positioning block 11 is pushed into the interior of the positioning groove. When the dripping frame 102 moves just above a dripping bucket 5 and corresponds to its position, the positioning hole on the dripping frame 102 is also just located at the corresponding positioning block 1 1, under the action of the positioning spring 10, the positioning block 11 is pushed into the interior of the positioning hole to limit the dropping frame 102 to a certain extent, so as to prevent the dropping frame 102 from moving when the sample or reagent is dropped into the detection cylinder 2, thereby ensuring the normal dripping of the sample or reagent, and at the same time ensuring that the shielding piece 103 stably shields other detection cylinders 2. A protective frame can be fixedly connected to the top of the dropping frame 102. When the dropping frame 102 is moved, the operator holds the outside of the protective frame to reduce the phenomenon that dirt on the operator's hands falls into the inside of the detection cylinder 2 when the operator moves the dropping frame 102.

[0057] In order to reduce the residual sample on the dripping frame 102 and the shielding piece 103 from entering the other detection cylinders 2, the top of the baffle 6 is detachably connected to a sponge pad 12, and the sponge pad 12 contacts the bottom of the dripping frame 102 and the shielding piece 103. The sponge pad 12 is detachably connected to the top of the baffle 6 through Velcro, and the sponge pad 12 can be easily replaced through Velcro. When the dripping frame 102 moves, the sponge pad 12 on the top of the baffle 6 cleans the dripping frame 102 and the shielding piece 103 to prevent the sample or reagent from splashing on the bottom of the dripping frame 102 and the shielding piece 103 and then moving to the top of other detection cylinders 2 and falling, thereby ensuring the accuracy of the test results. The sponge pad 12 can be disassembled and replaced regularly to ensure its water absorption.

[0058] It should be noted that in order to better mark the test samples, an identification plate 13 is also included. The identification plate 13 is provided with identification symbols "A, B, C, D, E, F, G, H" to mark the test tubes 2 in different columns, so as to facilitate rapid identification of sample numbers, thereby facilitating observation and recording of experimental results. The identification plate 13 is provided with a slide groove that matches the slider 4, and it can be installed on the bottom plate 1 at the edge to mark the enzyme-labeled wells.

[0059] The working principle or usage process of the bottom-sealed light-shielding ELISA plate is as follows:

[0060] According to the detection needs, select a suitable number of base plates 1, splice them together through the cooperation of the slider 4 and the slide groove, and install the identification plate 13 on the edge of the multiple base plates 1;

[0061] Install the detection cylinder 2 into the corresponding enzyme-labeled well, with the connecting block 3 located in the opening and the elastic protective sheet 9 sealing the opening;

[0062] Insert the insertion rod 201 into the slot. Under the action of the compression spring 204, the clamping block 202 is clamped into the slot to fix the insertion rod 201 and the shielding frame 101. Push the dropping frame 102 to move it above the dropping funnel 5 where the sample needs to be dropped. Drop the sample into the detection cylinder 2. Then continue to push the dropping frame 102 to move it above another dropping funnel 5 and continue to drop the sample. Repeat the above steps until the sample is dropped into all the detection cylinders 2. Move the dropping frame 102 to a position away from the dropping funnel 5.

[0063] The pull rod 203 can be pulled out of the card block 202 from the card slot, and the shielding frame 101 can be disassembled. The reagents can be sucked in through the drainage tube and multiple reagents can be dripped into the interior of multiple detection cylinders 2 at the same time. The baffle 6 and the partition 7 can protect the reagents from splashing during the dripping. Alternatively, the shielding frame 101 can be left undisassembled and the reagents can be dripped into the interior of multiple detection cylinders 2 in sequence by dripping the samples.

[0064] After the sample and reagents are added, proceed with the subsequent operations for detection and reading.

[0065] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present disclosure and are not limiting. Although the present disclosure has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present disclosure may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present disclosure, and all of these should be included in the scope of the claims of the present disclosure.

Claims

1. A light-shielding ELISA plate with a closed bottom, comprising a bottom plate (1) and a detection tube (2), characterized in that: The bottom plate (1) is provided with a plurality of enzyme-labeled wells, the detection cylinders (2) are located inside the enzyme-labeled wells, and the plurality of detection cylinders (2) in the same row are fixedly connected via a connecting block (3), and further comprising: A dripping hopper (5), the dripping hopper (5) being fixedly connected to the top end of the detection cylinder (2); baffles (6), wherein a plurality of baffles (6) are provided, a baffle groove matching the baffles (6) is provided on the bottom plate (1), the baffles (6) are slidably mounted inside the baffle groove, and the baffle groove is located between two adjacent enzyme labeling holes; A partition (7), wherein the partition (7) and the baffle (6) are fixedly connected, and the partition (7) and the bottom plate (1) are in sliding fit; A sealing structure is used to seal the tops of a plurality of detection cylinders (2) on the same bottom plate (1) and to keep the detection cylinder (2) into which the sample needs to be dripped in open. The sealing structure comprises: A shielding frame (101), the shielding frame (101) being detachably connected to the base plate (1); A dripping frame (102), the dripping frame (102) being slidably mounted on the shielding frame (101), both ends of the dripping frame (102) being fixedly connected to shielding sheets (103), and an end of the shielding sheet (103) away from the dripping frame (102) being fixedly connected to an inner wall of the shielding frame (101); The length of the shielding frame (101) is longer than the length of the bottom plate (1) by at least the length of the dripping frame (102); A support spring (8) is connected between the bottom end of the baffle (6) and the inner bottom wall of the baffle groove; The baffle (6) is provided with a through opening that matches the connecting block (3), and both side walls of the through opening are fixedly connected with a plurality of elastic protective sheets (9), which seal the through opening.

2. A bottom-sealed light-shielding ELISA plate according to claim 1, characterized in that: The shielding frame (101) is detachably connected to the base plate (1) via a connecting piece, wherein the connecting piece comprises: An insertion rod (201), the insertion rod (201) is fixedly connected to the bottom end of the shielding frame (101), and a slot matching the insertion rod (201) is provided on the bottom plate (1); A card block (202), a fixing groove is provided on the bottom plate (1), the fixing groove is communicated with the slot, the card block (202) is connected to the inside of the fixing groove via a compression spring (204), and a card slot matching the card block (202) is provided on the insertion rod (201); A pull rod (203) is fixedly connected to the clamping block (202), the pull rod (203) and the base plate (1) are in sliding engagement, and the pull rod (203) passes through the base plate (1) and extends to the outside.

3. A bottom-sealed light-shielding ELISA plate according to claim 2, characterized in that: One end of the base plate (1) is fixedly connected to a plurality of sliders (4), and the other end of the base plate (1) is provided with a plurality of sliding grooves matching the sliders (4).

4. A bottom-sealed light-shielding ELISA plate according to claim 3, characterized in that: The shielding frame (101) is provided with a plurality of positioning grooves, the positions of the positioning grooves corresponding to the positions of the enzyme-labeled holes, the interior of the positioning grooves is connected to a positioning block (11) via a positioning spring (10), and the dropping frame (102) is provided with a positioning hole matching the positioning block (11).

5. A bottom-sealed light-shielding ELISA plate according to claim 4, characterized in that: The top end of the baffle (6) is detachably connected to a sponge pad (12), and the sponge pad (12) is in contact with the bottom end of the dripping frame (102) and the shielding sheet (103).

6. A bottom-sealed light-shielding ELISA plate according to claim 5, characterized in that: The sponge pad (12) is detachably connected to the top end of the baffle (6) via Velcro.

7. A bottom-sealed light-shielding ELISA plate according to claim 6, characterized in that: A plurality of base plates (1) are provided, and the plurality of base plates (1) are detachably connected to each other.

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