Additive composition for feed

Abstract

Provided are a feed additive composition and a feed composition which are capable of promoting the digestion of proteins in fish, shellfish, etc. that have ingested a protein-containing feed. The feed additive composition contains (A) an anionic surfactant and (B) a nonionic surfactant, and the mass ratio [(A) / (B)] of the content of component (A) to the content of component (B) is 0.7-20.

Description

Technical Field

[0001] The present invention relates to a feed additive composition and a feed composition. Background Art

[0002] In recent years, in aquaculture, feeding pellets and other compound feeds for fish and shellfish has become the mainstream instead of live bait. In order to improve the quality of fish and shellfish, the development of better aquaculture feeds is being promoted.

[0003] Japanese Patent Publication No. 2003-235471 discloses a method for producing solid feed, characterized in that an emulsion obtained by emulsifying a water-soluble polymer aqueous solution and fish oil with an emulsifier (polyoxyethylene sorbitan fatty acid ester, etc.) is added to the feed raw material. Japanese Patent Publication No. 3-290156 discloses a method for producing dry granules, characterized in that when producing dry granules with a fat content of 10 to 50% by weight, 0.1 to 10% by weight of a nonionic surfactant (polyoxyethylene glycerol fatty acid ester, etc.) is added relative to the fat. Japanese Patent Application Laid-Open No. 59-66844 discloses a live bait feed composition, wherein the live bait contains thiamine or thiamine salts coated with a coating agent containing 1 / 3 to 1 times the amount of fat and lecithin with a melting point of 50 to 80°C or fat and lecithin with a melting point of 50 to 80°C, or fat, lecithin and polyglycerol polymerized fatty acid ester with a melting point of 50 to 80°C. The feed composition can prevent thiamine deficiency in fish. Summary of the invention

[0004] As an example of the development of feed for aquaculture of fish and shellfish (hereinafter referred to as fish farming feed), a method for achieving weight gain of individual fish and shellfish can be cited, in which it is necessary to promote the digestion of protein in the fish and shellfish. Therefore, the subject of the present invention is to provide a feed additive composition and a feed composition that can promote the digestion of protein in fish and shellfish that have ingested a feed containing protein.

[0005] In one embodiment, the present invention provides a feed additive composition comprising (A) an anionic surfactant [hereinafter referred to as component (A)] and (B) a nonionic surfactant [hereinafter referred to as component (B)], wherein the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is 0.7 or more and 20 or less.

[0006] In another embodiment, the present invention provides a feed composition comprising (A) an anionic surfactant [hereinafter referred to as component (A)], (B) a nonionic surfactant [hereinafter referred to as component (B)], and (C) a protein [hereinafter referred to as component (C)], wherein the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is 0.7 or more and 20 or less.

[0007] According to the feed additive composition and the feed composition of the present invention, it is possible to promote the digestion of protein in fish and shellfish that have ingested a feed containing protein, thereby improving the quality of the fish and shellfish. DETAILED DESCRIPTION

[0008] [Feed additive composition]

[0009] The feed additive composition of the present invention comprises (A) an anionic surfactant and (B) a nonionic surfactant, wherein the mass ratio [(A) / (B)] of the content of the component (A) to the content of the component (B) is 0.7 or more and 20 or less.

[0010] In the feed additive composition of the present invention, the component (A) acts on protein to change the structure of the protein, thereby improving digestibility.

[0011] Examples of the component (A) include at least one selected from alkyl sulfates, alkylbenzenesulfonates, alkylsulfosuccinates, and cholates. The salt of the component (A) is preferably an alkali metal salt such as a sodium salt or a potassium salt, and may also be a thiamine salt.

[0012] Regarding the alkyl sulfate, from the viewpoint of improving digestibility, the carbon number of the alkyl group is preferably 8 or more, more preferably 10 or more, and may be 18 or less, more preferably 16 or less. The alkyl group of the alkyl sulfate may be either linear or branched, but from the viewpoint of improving digestibility, the alkyl group is preferably linear.

[0013] In the present invention, an alkyl sulfate having a linear alkyl group having 10 or more carbon atoms and 16 or less carbon atoms is preferred. Examples of the salt constituting the alkyl sulfate include alkali metal salts such as sodium and potassium, alkanolamine salts, and thiamine salts. However, from the viewpoint of improving digestibility, sodium salts are preferred, and from the viewpoint of improving edibility, thiamine salts are preferred.

[0014] Thiamine is also known as vitamin B1, and it is known to add vitamin B1 to feed for the purpose of producing livestock with increased vitamin B1 content. However, there is no known technology for adding anionic surfactants such as thiamine salts to feed to promote digestion of protein-containing feed.

[0015] Regarding the alkylbenzene sulfonate, from the viewpoint of improving digestibility, the alkyl group preferably has 8 or more carbon atoms, more preferably 10 or more carbon atoms, and preferably has 18 or less carbon atoms, more preferably has 16 or less carbon atoms. The alkyl group of the alkylbenzene sulfonate may be either linear or branched, but from the viewpoint of improving digestibility, the alkyl group is preferably linear.

[0016] In the present invention, the alkylbenzene sulfonate is preferably an alkylbenzene sulfonate having a linear alkyl group having 10 or more carbon atoms and 16 or less carbon atoms. Examples of the salt constituting the alkylbenzene sulfonate include alkali metal salts such as sodium and potassium, and thiamine salts. However, from the viewpoint of improving digestibility, sodium salts are preferred, and from the viewpoint of improving edibility, thiamine salts are preferred.

[0017] Regarding alkyl sulfosuccinates, from the viewpoint of improving digestibility, the carbon number of the alkyl group is preferably 6 or more, more preferably 8 or more, and preferably 18 or less, and more preferably 16 or less. The alkyl group of the alkyl sulfosuccinate may be any of a straight chain and a branched one, and from the viewpoint of improving digestibility, the alkyl group is preferably a straight chain. The alkyl sulfosuccinate is preferably a diester-type compound having two alkyl groups (hereinafter referred to as dialkyl sulfosuccinate). Regarding dialkyl sulfosuccinates, from the viewpoint of improving digestibility, the total carbon number of the two alkyl groups is preferably 10 or more, more preferably 12 or more, and preferably 24 or less, and more preferably 20 or less. The carbon numbers of the two alkyl groups of the dialkyl sulfosuccinate may be the same or different. The salts constituting the alkyl sulfosuccinates and dialkyl sulfosuccinates include alkali metal salts such as sodium and potassium, but from the viewpoint of improving digestibility, sodium salts are preferred.

[0018] Examples of the salt constituting bile salt include alkali metal salts such as sodium and potassium. From the viewpoint of improving digestibility, sodium salt is preferred.

[0019] As for the component (A), sodium lauryl sulfate and thiamine lauryl sulfate are preferred from the viewpoint of improving digestibility. Also, thiamine lauryl sulfate is preferred from the viewpoint of improving ingestibility.

[0020] The feed additive composition of the present invention determines the amount of each component to be used based on the total amount of protein in the feed to which the composition is added. Regarding the content of component (A), from the viewpoint of improving digestibility, relative to 100 parts by mass of protein in the feed, it is preferably 0.1 parts by mass or more, more preferably 0.2 parts by mass or more, further preferably 0.5 parts by mass or more, further preferably 1 part by mass or more, further preferably 2 parts by mass or more, further preferably 3 parts by mass or more, and from the viewpoint of improving the ingestibility and economic efficiency, it is preferably 50 parts by mass or less, more preferably 40 parts by mass or less, more preferably 30 parts by mass or less, further preferably 20 parts by mass or less, further preferably 15 parts by mass or less. The amount of the anionic surfactant of component (A) is based on the amount of the compound in which the counter ion is replaced by the sodium ion.

[0021] The feed additive composition of the present invention contains a nonionic surfactant as the component (B).

[0022] From the viewpoint of improving digestibility, the component (B) is preferably a nonionic surfactant having an HLB of 6 or more and 18 or less. From the viewpoint of improving digestibility, the HLB of the component (B) is preferably 6 or more, more preferably 8 or more, further preferably 10 or more, further preferably 13 or more, and is preferably 18 or less, more preferably 17 or less, further preferably 16.5 or less.

[0023] In the present invention, a nonionic surfactant having an HLB of 10 or more and an HLB of 17 or less is preferred.

[0024] The HLB of the component (B) can be determined using the Griffin's formula shown below.

[0025] HLB = 20 × (the sum of the formula weights of the hydrophilic part of the nonionic surfactant) / (the molecular weight of the nonionic surfactant)

[0026] When two or more nonionic surfactants are contained, the HLB of the component (B) is determined as a weighted average.

[0027] Examples of the component (B) include at least one selected from polyoxyethylene alkyl ethers, sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene fatty acid esters, sucrose fatty acid esters, glycerol fatty acid esters, polyoxyethylene glycerol fatty acid esters, and polyoxyethylene hydrogenated castor oil.

[0028] Regarding polyoxyethylene alkyl ether, from the viewpoint of improving digestibility, the alkyl group preferably has 8 or more carbon atoms, more preferably has 10 or more carbon atoms, and preferably has 20 or less carbon atoms, more preferably has 18 or less carbon atoms, and more preferably has 16 or less carbon atoms. The alkyl group of polyoxyethylene alkyl ether may be either a straight chain or a branched group, and from the viewpoint of improving digestibility, the alkyl group is preferably a straight chain. Regarding the average number of moles of oxyethylene groups added to polyoxyethylene alkyl ether, from the viewpoint of improving digestibility, it is preferably 2 or more, more preferably 8 or more, and preferably 50 or less, more preferably 45 or less, and further preferably 40 or less.

[0029] In the present invention, polyoxyethylene alkyl ethers having an alkyl group having 10 or more and 16 or less carbon atoms and having an average number of oxyethylene groups added of 8 or more and 40 or less moles are preferred.

[0030] The fatty acid in the structural unit derived from fatty acid constituting sorbitan fatty acid ester and polyoxyethylene sorbitan fatty acid ester is preferably 8 or more carbon atoms, more preferably 10 or more carbon atoms, and preferably 20 or less carbon atoms, more preferably 18 or less carbon atoms, from the viewpoint of improving digestibility. The fatty acid in the structural unit derived from fatty acid constituting sorbitan fatty acid ester and polyoxyethylene sorbitan fatty acid ester may be any of straight chain and branched, preferably straight chain fatty acid. In addition, the above-mentioned fatty acid may be any of saturated or unsaturated fatty acids, and mixed fatty acids may be used.

[0031] Examples of the ester constituting the sorbitan fatty acid ester and the polyoxyethylene sorbitan fatty acid ester include monoesters, diesters, and triesters, and monoesters are preferred.

[0032] The average added molar number of oxyethylene groups in the polyoxyethylene sorbitan fatty acid ester is preferably 2 or more, more preferably 5 or more, and is preferably 40 or less, more preferably 25 or less, from the viewpoint of improving digestibility.

[0033] In the present invention, polyoxyethylene sorbitan fatty acid (mono)esters composed of a straight-chain fatty acid having 10 to 18 carbon atoms and having an average added mole number of oxyethylene groups of 5 to 25 are preferred.

[0034] Regarding the fatty acid in the structural unit derived from fatty acid constituting the polyoxyethylene fatty acid ester, from the viewpoint of improving digestibility, it is preferably more than 6 carbon numbers, more preferably more than 8, and preferably less than 20 carbon numbers, more preferably less than 18 carbon numbers. The fatty acid in the structural unit derived from fatty acid constituting the polyoxyethylene fatty acid ester can be any one of straight chain and branched, preferably a straight chain fatty acid. In addition, the above-mentioned fatty acid can be any one of saturated or unsaturated fatty acids, and mixed fatty acids can be used. Regarding the average addition mole number of the oxyethylene group of the polyoxyethylene fatty acid ester, from the viewpoint of improving digestibility, it is preferably more than 2, more preferably more than 10, and preferably less than 50, more preferably less than 40.

[0035] In the present invention, a polyoxyethylene fatty acid ester composed of a straight-chain fatty acid having 8 to 18 carbon atoms and having an average added mole number of oxyethylene groups of 10 to 40 is preferred.

[0036] The fatty acid in the structural unit derived from fatty acid constituting the sucrose fatty acid ester is preferably 8 or more carbons, more preferably 10 or more carbons, and preferably 20 or less carbons, more preferably 18 or less carbons, from the viewpoint of improving digestibility. The fatty acid in the structural unit derived from fatty acid constituting the sucrose fatty acid ester may be any of straight chain and branched, preferably straight chain fatty acid. In addition, the above-mentioned fatty acid may be any of saturated or unsaturated fatty acids, and a mixed fatty acid may be used.

[0037] In the present invention, sucrose fatty acid esters composed of a straight-chain fatty acid having 8 or more and 18 or less carbon atoms are preferred.

[0038] Regarding the fatty acid in the structural unit derived from fatty acids constituting glycerol fatty acid esters and polyoxyethylene glycerol fatty acid esters, from the viewpoint of improving digestibility, it is preferably a carbon number of 6 or more, more preferably a carbon number of 8 or more, and preferably a carbon number of 20 or less, more preferably a carbon number of 18 or less. Regarding the fatty acid in the structural unit derived from fatty acids constituting glycerol fatty acid esters and polyoxyethylene glycerol fatty acid esters, from the viewpoint of improving digestibility, it can be any of straight chain and branched, preferably a straight chain fatty acid. In addition, the above-mentioned fatty acid can be any of saturated or unsaturated fatty acids, and mixed fatty acids can be used. The esters constituting glycerol fatty acid esters and polyoxyethylene glycerol fatty acid esters can include monoesters, diesters, and triesters, preferably monoesters.

[0039] The average added molar number of oxyethylene groups in the polyoxyethylene glycerol fatty acid ester is preferably 2 or more, more preferably 10 or more, and is preferably 50 or less, more preferably 40 or less, from the viewpoint of improving digestibility.

[0040] In the present invention, polyoxyethylene glycerol fatty acid esters composed of a straight-chain fatty acid having 8 to 18 carbon atoms and having an average number of oxyethylene groups added of 10 to 40 moles are preferred.

[0041] The average added mole number of oxyethylene groups in the polyoxyethylene hydrogenated castor oil is preferably 2 or more, more preferably 10 or more, further preferably 20 or more, and is preferably 50 or less, more preferably 45 or less, from the viewpoint of improving digestibility.

[0042] As the component (B), from the viewpoint of improving digestibility, a nonionic surfactant having an oxyethylene group and having an HLB of 6 to 18 is preferred, and examples thereof include polyoxyethylene alkyl ethers, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene fatty acid esters, polyoxyethylene glycerol fatty acid esters, and polyoxyethylene hydrogenated castor oil. From the viewpoint of improving digestibility, polyoxyethylene alkyl ethers, polyoxyethylene sorbitan fatty acid esters, and polyoxyethylene glycerol fatty acid esters are preferred.

[0043] Moreover, as (B)component, specifically, polyoxyethylene lauryl ether, sorbitan laurate, polyoxyethylene sorbitan laurate, polyoxyethylene sorbitan oleate, capric acid monoglyceride etc. are mentioned.

[0044] As the component (B), from the viewpoint of improving digestibility, preferably, polyoxyethylene lauryl ether and polyoxyethylene sorbitan oleate are used. From the viewpoint of improving ingestibility, preferably, polyoxyethylene sorbitan laurate, polyoxyethylene sorbitan oleate and capric acid monoglyceride are used.

[0045] The feed additive composition of the present invention determines the amount of each component to be used based on the total amount of protein in the feed to which the composition is added. The content of the component (B) is preferably 0.1 parts by mass or more, more preferably 0.2 parts by mass or more, further preferably 0.5 parts by mass or more, further preferably 1 part by mass or more, further preferably 2 parts by mass or more, from the viewpoint of improving digestibility and economic efficiency, relative to 100 parts by mass of protein in the feed. Furthermore, from the viewpoint of improving edibility and economic efficiency, it may be 20 parts by mass or less, preferably 15 parts by mass or less.

[0046] In the feed additive composition of the present invention, from the viewpoint of improving digestibility, [(A) / (B)], which is the mass ratio of the content of the component (A) to the content of the component (B), may be 0.7 or more, preferably 0.9 or more, more preferably 1.0 or more, and even more preferably 2.0 or more, and may be 20 or less, preferably 10 or less, and even more preferably 6 or less. In addition, from the viewpoint of improving feedability, [(A) / (B)], which is the mass ratio of the content of the component (A) to the content of the component (B), may be 20 or less, preferably 10 or less, more preferably 8 or less, and even more preferably 5.0 or less.

[0047] The feed additive composition of the present invention can be prepared by mixing the anionic surfactant of the component (A) and the nonionic surfactant of the component (B) at a mass ratio [(A) / (B)] of 0.7 to 20.

[0048] The feed additive composition of the present invention may be in the form of a solid or a liquid.

[0049] [Feed composition]

[0050] The feed composition of the present invention contains the above-mentioned feed additive composition and protein. In addition, the feed composition of the present invention is a feed composition containing (A) anionic surfactant, (B) nonionic surfactant and (C) protein, and the mass ratio [(A) / (B)] of the content of the component (A) to the content of the component (B) is 0.7 or more and 20 or less. Specific examples, preferred examples, etc. of the component (A) and the component (B) of the feed composition of the present invention may be the same as those of the above-mentioned feed additive composition of the present invention.

[0051] The protein of component (C) may be vegetable protein or animal protein, or may contain both vegetable protein and animal protein. As the protein source of vegetable protein, for example, soybean meal, corn gluten meal, corn, sorghum, barley, wheat, cassava, wheat bran, rice bran, rapeseed oil meal, rice for feed, etc. can be cited, but it is not particularly limited. However, from the viewpoint of economy, soybean meal is preferred. As the protein source of animal protein, for example, fish meal, blended fish meal, fish residue, skimmed milk powder, whey powder, chicken meal, feather meal, meat meal / meat and bone meal, etc. can be cited.

[0052] The feed composition of the present invention preferably contains a vegetable protein as the component (C), and is preferably a feed composition containing a vegetable protein.

[0053] The feed composition of the present invention preferably contains vegetable protein and animal protein as the component (C).

[0054] From the viewpoint of improving growth performance, the feed composition of the present invention may contain, among the proteins contained in the above-mentioned composition, i.e., in the component (C), preferably 5% by mass or more, more preferably 10% by mass or more, and even more preferably 20% by mass or more of animal protein, and from the viewpoint of economic efficiency, preferably 80% by mass or less, more preferably 60% by mass or less, and even more preferably 50% by mass or less of animal protein.

[0055] When the feed composition of the present invention contains vegetable protein and animal protein as component (C), the mass ratio of the content of animal protein to the total amount of protein in the feed composition [animal protein / protein] may be, for example, 0.1 or more, more preferably 0.2 or more, and even more preferably 0.4 or more from the viewpoint of improving digestibility, and may be 0.8 or less, preferably 0.7 or less, and even more preferably 0.6 or less from the viewpoint of economic efficiency.

[0056] The feed composition of the present invention preferably contains 10% by mass or more, more preferably 20% by mass or more, further preferably 30% by mass or more, further preferably 40% by mass or more of the component (C), and preferably contains 99% by mass or less, more preferably 97% by mass or less, further preferably 90% by mass or less, further preferably 80% by mass or less, further preferably 70% by mass or less, further preferably 60% by mass or less of the component (C), from the viewpoint of growth performance.

[0057] In the feed composition of the present invention, from the viewpoint of improving digestibility, the component (A) is preferably contained in an amount of 0.1 parts by mass or more, more preferably 0.2 parts by mass or more, further preferably 0.5 parts by mass or more, further preferably 1 part by mass or more, further preferably 2 parts by mass or more, further preferably 3 parts by mass or more, based on 100 parts by mass of the component (C). Furthermore, from the viewpoint of improving feedability and economic efficiency, the component (A) may be contained in an amount of 50 parts by mass or less, preferably 40 parts by mass or less, more preferably 30 parts by mass or less, further preferably 20 parts by mass or less, further preferably 15 parts by mass or less.

[0058] The feed composition of the present invention may contain 0.1 parts by mass or more, preferably 0.2 parts by mass or more, more preferably 0.5 parts by mass or more, further preferably 1 part by mass or more, and further preferably 2 parts by mass or more of the component (B) relative to 100 parts by mass of the component (C) from the viewpoint of improving digestibility, and may contain 20 parts by mass or less, preferably 15 parts by mass or less of the component (B) from the viewpoint of improving feedability and economic efficiency.

[0059] In the feed composition of the present invention, from the viewpoint of improving digestibility, [(A) / (B)], which is the mass ratio of the content of the component (A) to the content of the component (B), may be 0.7 or more, preferably 0.9 or more, more preferably 1.0 or more, and even more preferably 2.0 or more, and may be 20 or less, preferably 10 or less, and even more preferably 6 or less. Furthermore, from the viewpoint of improving feedability, [(A) / (B)], which is the mass ratio of the content of the component (A) to the content of the component (B), may be 20 or less, preferably 10 or less, more preferably 8 or less, even more preferably 5.0 or less, and even more preferably 4.0 or less.

[0060] The feed composition of the present invention may contain the following components as optional components in addition to the components (A), (B) and (C). Examples include excipients such as starch and carboxymethyl cellulose, vitamins (excluding vitamin B1), minerals such as ash, dietary fiber, lipids, carbohydrates, yeasts such as torula yeast, amino acids such as methionine, etc. The optional components may include surfactants other than the components (A) and (B), namely, cationic surfactants, amphoteric surfactants, etc.

[0061] In the feed composition of the present invention, the total content of the component (A) and the component (B) is preferably 80% by mass or more, more preferably 90% by mass or more, and even more preferably 95% by mass or more in terms of 100% by mass of all surfactants contained in the feed composition.

[0062] The feed composition of the present invention can be produced by mixing the components (A) and (B) with the component (C) so that the mass ratio [(A) / (B)] of the content of the component (A) to the content of the component (B) is in the range of 0.7 or more and 20 or less.

[0063] The feed composition of the present invention can be produced by adding the component (A) in an amount of 0.1 to 50 parts by mass, the component (B) in an amount of 0.1 to 20 parts by mass, and the mass ratio [(A) / (B)] of the component (A) to the component (B) to 100 parts by mass of the component (C) so as to be 0.7 to 20, and adding other components as necessary, followed by molding, etc.

[0064] The form of the feed composition of the present invention is not limited, and examples thereof include solid forms, particularly granules, fine particles, and powders. Examples of granules include wet granules, dry granules, single-grain wet granules, and extruded granules.

[0065] When the feed composition of the present invention is a solid feed composition for fish farming, the particle size (longer diameter) of the solid feed composition can be adjusted according to the target fish species, and is, for example, preferably 0.5 mm or more, more preferably 1.0 mm or more.

[0066] When the solid feed composition of the present invention is a single wet granule or wet granule, the water content in 100% by mass of the above composition is preferably 10% by mass or more, more preferably 20% by mass, from the viewpoint of improving shape retention and granulation properties, and is preferably 70% by mass or less, more preferably 60% by mass or less from the viewpoint of improving storage properties and economic efficiency. When the solid feed composition of the present invention is a dry granule or extruded granule, the water content in 100% by mass of the above composition is preferably 1% by mass or more, more preferably 3% by mass, from the viewpoint of improving shape retention and granulation properties, and is preferably 40% by mass or less, more preferably 30% by mass or less from the viewpoint of improving storage properties and economic efficiency.

[0067] The feed composition of the present invention is preferably a feed composition for fish farming. As fish and shellfish fed with the feed composition for fish farming of the present invention, fish are preferably fish, for example, yellowtail, hamachi (young yellowtail), red sea bream, greater yellowtail, tuna, flounder, horse mackerel, horse mackerel, redfin pufferfish, yellowtail, striped sea bream, pufferfish, black sea bream, brown-banded grouper, white mackerel, seven-banded grouper, etc. can be mentioned without particular limitation.

[0068] [Fish and Shellfish Farming Methods]

[0069] The present invention provides a method for cultivating fish and shellfish, wherein the feed composition is fed to the cultivated fish and shellfish. The method for cultivating fish and shellfish in the present invention can include a conventional method for cultivating fish and shellfish, in addition to feeding the fish farming feed containing the feed additive composition of the present invention and the feed composition of the present invention.

[0070] [Protein digestion promoting agent composition]

[0071] The present invention has found that if an anionic surfactant as component (A) and a nonionic surfactant as component (B) are contained in a composition containing protein at a mass ratio [(A) / (B)] of 0.7 or more and 20 or less, then the digestion of protein ingested from feed can be promoted in livestock such as fish, shellfish, chickens, pigs, and cattle. That is, the present invention provides a protein digestion promoter composition, which is a protein digestion promoter comprising (A) anionic surfactant and (B) nonionic surfactant, and the content of component (A) and the content of component (B) are contained at a mass ratio [(A) / (B)] of 0.7 or more and 20 or less. The protein digestion promoter composition of the present invention can be used for fish farming, chicken farming, pig farming, and cattle farming, and is preferably used for fish farming.

[0072] The protein digestion promoter composition of the present invention may be a vegetable protein digestion promoter composition, a protein digestion promoter composition for fish farming, or a vegetable protein digestion promoter composition for fish farming. In the promoter composition of the present invention, specific examples and preferred examples of the components (A) and (B) may be the same as those of the feed additive composition and feed composition of the present invention described above.

[0073] The protein digestion promoting composition of the present invention can be confirmed to function as a protein digestion promoting composition by, for example, investigating the protein degradation rate in in vitro or in vivo tests as shown in the examples described below. The protein digestion promoting composition of the present invention can be prepared in the same manner as the feed additive composition of the present invention.

[0074] The present invention discloses the following feed additive composition, feed composition, fish and shellfish farming method and protein digestion promoter composition.

[0075] <1>

[0076] A feed additive composition comprising (A) an anionic surfactant [hereinafter referred to as component (A)] and (B) a nonionic surfactant [hereinafter referred to as component (B)], wherein the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is 0.7 or more and 20 or less.

[0077] <2>

[0078] The feed additive composition according to the above <1>, wherein the component (A) is an anionic surfactant selected from the group consisting of alkyl sulfates, alkylbenzenesulfonates, alkylsulfosuccinates and bile salts.

[0079] <3>

[0080] The feed additive composition according to <1> or <2>, comprising 0.1 parts by mass or more and 50 parts by mass or less of the component (A) based on 100 parts by mass of protein in the feed.

[0081] <4>

[0082] The feed additive composition according to any one of <1> to <3>, wherein the HLB of the component (B) is 6 or more and 18 or less.

[0083] <5>

[0084] The feed additive composition according to any one of <1> to <4>, wherein the HLB of the component (B) is 6 or more, more preferably 8 or more, more preferably 10 or more, more preferably 13 or more, and is 18 or less, more preferably 17 or less, more preferably 16.5 or less.

[0085] <6>

[0086] The feed additive composition according to any one of <1> to <5>, wherein the component (B) is a nonionic surfactant selected from the group consisting of polyoxyethylene alkyl ethers, sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene fatty acid esters, sucrose fatty acid esters, glycerol fatty acid esters, polyoxyethylene glycerol fatty acid esters and polyoxyethylene hydrogenated castor oil.

[0087] <7>

[0088] The feed additive composition according to any one of <1> to <6>, comprising 0.1 parts by mass or more and 20 parts by mass or less of the component (B) based on 100 parts by mass of protein in the feed.

[0089] <8>

[0090] The feed additive composition according to any one of <1> to <7>, which contains 0.1 parts by mass or more, further preferably 0.2 parts by mass or more, further preferably 0.5 parts by mass or more, further preferably 1 part by mass or more, further preferably 2 parts by mass or more of the component (B) relative to 100 parts by mass of protein in the feed, and contains 20 parts by mass or less, further preferably 15 parts by mass or less of the component (B).

[0091] <9>

[0092] The feed additive composition according to any one of <1> to <8>, wherein [(A) / (B)], which is the mass ratio of the content of the component (A) to the content of the component (B), is 0.7 or more, further preferably 0.9 or more, further preferably 1.0 or more, further preferably 2.0 or more, and is 20 or less, further preferably 10 or less, further preferably 8 or less, further preferably 6 or less, further preferably 5.0 or less.

[0093] <10>

[0094] The feed additive composition according to any one of <1> to <9>, which is a feed for farming of fish and shellfish.

[0095] <11>

[0096] A feed composition comprising (A) an anionic surfactant [hereinafter referred to as component (A)], (B) a nonionic surfactant [hereinafter referred to as component (B)], and (C) a protein [hereinafter referred to as component (C)], wherein the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is 0.7 or more and 20 or less.

[0097] <12>

[0098] The feed composition according to the above <11>, wherein the component (A) is an anionic surfactant selected from the group consisting of alkyl sulfates, alkylbenzenesulfonates, alkylsulfosuccinates and bile salts.

[0099] <13>

[0100] The feed composition according to <11> or <12>, comprising 0.1 parts by mass or more and 50 parts by mass or less of the component (A) based on 100 parts by mass of the component (C).

[0101] <14>

[0102] The feed composition according to any one of <11> to <13>, wherein the HLB of the component (B) is 6 or more and 18 or less.

[0103] <15>

[0104] The feed composition according to any one of <11> to <14>, wherein the component (B) is a nonionic surfactant selected from polyoxyethylene alkyl ethers, sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene fatty acid esters, sucrose fatty acid esters, glycerol fatty acid esters, polyoxyethylene glycerol fatty acid esters and polyoxyethylene hydrogenated castor oil.

[0105] <16>

[0106] The feed composition according to any one of <11> to <15>, comprising 0.1 parts by mass or more and 20 parts by mass or less of the component (B) based on 100 parts by mass of the component (C).

[0107] <17>

[0108] The feed composition according to any one of <11> to <16>, wherein the component (C) contains a vegetable protein.

[0109] <18>

[0110] The feed composition according to any one of <11> to <17>, which contains 10% by mass or more, further preferably 20% by mass or more, further preferably 30% by mass or more, further preferably 40% by mass or more of the component (C), and contains 99% by mass or less, further preferably 97% by mass or less, further preferably 90% by mass or less, further preferably 80% by mass or less, further preferably 70% by mass or less, further preferably 60% by mass or less of the component (C).

[0111] <19>

[0112] The feed composition according to any one of <11> to <18>, which contains the component (A) in an amount of further 0.1 parts by mass or more, further 0.2 parts by mass or more, further 0.5 parts by mass or more, further 1 part by mass or more, further 2 parts by mass or more, further 3 parts by mass or more, and the component (A) is contained in an amount of no more than 50 parts by mass, further 40 parts by mass or less, further 30 parts by mass or less, further 20 parts by mass or less, further 15 parts by mass or less, based on 100 parts by mass of the component (C).

[0113] <20>

[0114] The feed composition according to any one of <11> to <19>, which contains 0.1 parts by mass or more, further preferably 0.2 parts by mass or more, further preferably 0.5 parts by mass or more, further preferably 1 part by mass or more, further preferably 2 parts by mass or more of the component (B) relative to 100 parts by mass of the component (C), and contains 20 parts by mass or less, further preferably 15 parts by mass or less of the component (B).

[0115] <21>

[0116] The feed composition according to any one of <11> to <20>, wherein [(A) / (B)] which is the mass ratio of the content of the component (A) to the content of the component (B) is 0.7 or more, further preferably 0.9 or more, further preferably 1.0 or more, further preferably 2.0 or more, and is 20 or less, further preferably 10 or less, further preferably 8 or less, further preferably 6 or less, further preferably 5.0 or less, further preferably 4.0 or less.

[0117] <22>

[0118] The feed composition according to any one of <11> to <21>, which is a feed for farming fish and shellfish.

[0119] <23>

[0120] A method for cultivating fish and shellfish, comprising feeding the feed composition according to any one of <11> to <22> to fish and shellfish.

[0121] <24>

[0122] A protein digestion-promoting agent composition for fish farming, comprising (A) an anionic surfactant [hereinafter referred to as component (A)], (B) a nonionic surfactant [hereinafter referred to as component (B)], and (C) a protein [hereinafter referred to as component (C)], wherein the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is 0.7 or more and 20 or less.

[0123] <25>

[0124] A method for producing a feed composition, comprising mixing (A) anionic surfactant [hereinafter referred to as component (A)], (B) nonionic surfactant [hereinafter referred to as component (B)], and (C) protein [hereinafter referred to as component (C)] so that the mass ratio [(A) / (B)] of the content of component (A) to the content of component (B) is in the range of 0.7 or more and 20 or less.

[0125] <26>

[0126] A method for cultivating fish and shellfish, comprising feeding the cultivated fish and shellfish with a feed composition comprising (A) anionic surfactant [hereinafter referred to as (A) component], (B) nonionic surfactant [hereinafter referred to as (B) component] and (C) protein [hereinafter referred to as (C) component], wherein the mass ratio of the content of the component (A) to the content of the component (B) [(A) / (B)] is within a range of 0.7 or more and 20 or less.

[0127] Example

[0128] <Example 1 and Comparative Example 1>

[0129] [Protein digestibility (in vitro test)]

[0130] (1) Preparation of feed composition

[0131] To soybean meal (manufactured by Showa Sangyo Co., Ltd., containing 43% by mass of protein as component (C)) as a protein source, component (A) and component (B) were added in the amounts shown in Table 1, and the mixture was stirred in a mortar for about 5 minutes to prepare a feed composition.

[0132] (2) Preparation of enzyme extracted from red sea bream

[0133] Purified water was added to the intestines collected from red sea bream (Pagrus major) at a mass ratio of 1:1, and the intestines were pulverized with a blender and filtered. The obtained filtrate was used as red sea bream extracted enzyme.

[0134] (3) Calculation of protein degradation rate

[0135] The feed composition prepared in (1) with 10 mg of protein derived from soybean meal, 50 μL of red sea bream extract enzyme, and 5 mL of phosphate buffer (pH 8) were added to a 30 mL pear-shaped flask and stirred to prepare a crude treated liquid. After 24 hours, 100 μL of the crude treated liquid was added to a sampling tube (1.5 mL volume), and the sampling tube was immersed in boiling water and boiled for 5 minutes in a manner such that the entire amount of the crude treated liquid in the sampling tube was heated. The heated sampling tube was centrifuged (10,000 rpm, 5 minutes) to deproteinize the crude treated liquid to obtain a protein digestion treated liquid (hereinafter referred to as the treated liquid).

[0136] Trinitrobenzenesulfonic acid (Trinitro Benzenesulfonic acid, manufactured by TCI) was added to the above-mentioned treatment liquid and reacted at 60°C for 1 hour. Hydrochloric acid was added and stirred to stop the reaction. The absorbance of the obtained treatment liquid at 420nm was measured. The absorbance at 420nm was measured, and a standard curve was drawn using tyrosine to calculate the protein degradation rate. The digestibility of the protein was evaluated as a relative value relative to the protein degradation rate in Comparative Example 1. The results are shown in Table 1. The larger the digestibility value in Table 1, the more it can promote the digestion of protein.

[0137] [Table 1]

[0138]

[0139] ※1: Mass parts per 100 parts by mass of protein source (soybean meal)

[0140] ※2: Content when 43% by mass of (C) protein contained in the protein source (soybean meal) is assumed to be 100 parts by mass

[0141] ※3: Relative value when Comparative Example 1-1 is set as 100

[0142] The components in the table are the following substances. (B) The HLB of the component was calculated by the Griffin method.

[0143] <Ingredients>

[0144] (A) Ingredient: Anionic surfactant

[0145] ・Sodium lauryl sulfate: EMAL 10PT, Kao Corporation

[0146] ・Ammonium lauryl sulfate: EMAL AD-25R, Kao Corporation

[0147] ・Thiamine lauryl sulfate: FINE VB-100, Tsuruya Chemical Industry Co., Ltd.

[0148] ・Sodium lauryl benzene sulfonate: NEOPELEX G20, Kao Corporation

[0149] ・Sodium cholate: Fujifilm Wako Pure Chemical Industries, Ltd.

[0150] (B) Ingredient: Nonionic surfactant

[0151] ・POE (2) C12 ether: Polyoxyethylene (2) lauryl ether (the numbers in parentheses are the average number of added moles, the same below), Kao Corporation (HLB 6.3)

[0152] ・POE (12) C12 ether: Polyoxyethylene (12) lauryl ether, Kao Corporation (HLB 15.3)

[0153] ・POE (43) C12 ether: Polyoxyethylene (43) lauryl ether, Kao Corporation (HLB 16.3)

[0154] ・C12 sorbitan fatty acid ester: RHEODOL SP-L10, Kao Corporation (HLB8.6)

[0155] ・C12EO (6) sorbitan fatty acid ester: RHEODOL TW-L106, Kao Corporation (HLB13.3)

[0156] ・C12EO (20) sorbitan fatty acid ester: RHEODOL TW-L120, Kao Corporation (HLB16.7)

[0157] ・C18:1EO (6) Sorbitan fatty acid ester: RHEODOL TW-O106, Kao Corporation (HLB10)

[0158] ・C18:1EO (20) sorbitan fatty acid ester: RHEODOL TW-O120, Kao Corporation (HLB15)

[0159] ・C10 monoglyceride: SUNSOFT 760H, Taiyo Chemical Co., Ltd. (HLB 6.5)

[0160] <Example 2 and Comparative Example 2>

[0161] [Protein digestibility (in vitro test)]

[0162] The preparation of the feed composition, the preparation of the red sea bream extract enzyme, and the calculation of the protein degradation rate were performed in the same manner as in Example 1 and Comparative Example 1, except that the soybean meal as the protein source in Example 1 was replaced with the protein source described in Table 2, and the (A) component and the (B) component were added in the amounts described in Table 2. The (A) component and the (B) component used were the same as in Example 1 and Comparative Example 1. The results are shown in Table 2.

[0163] [Table 2]

[0164]

[0165] ※1: Mass parts per 100 parts by mass of protein source

[0166] ※2: Content based on 100 parts by mass of component (C) (net protein content) contained in the protein source

[0167] ※3: Relative value when n is 100 in Comparative Example 2 (n=1 to 5)

[0168] <Example 3 and Comparative Example 3>

[0169] [Growth (in vivo test)]

[0170] (1) Preparation of feed composition

[0171] To a mixture of 25 g of soybean meal (manufactured by Showa Sangyo Co., Ltd., containing 43% by mass of protein as component (C)) and 40 g of fish meal (manufactured by Yoshida Feed Co., Ltd., containing 61% by mass of protein as component (C)) as a protein source, 50 g of tap water was added and the components (A) and (B) described in Table 3 were added in the amounts shown in Table 3, respectively. After being fully mixed, the mixture was formed using a single-screw extruder granulator (rotation speed 50 rpm) with a screen diameter of 3 mm to form single-grain wet granules. The moisture content was 42 to 44%. It should be noted that the components (A) and (B) used were the same as those in Example 1 and Comparative Example 1. The moisture content was determined using a halogen moisture meter (manufactured by METTLER) to measure the moisture content in 1 g of feed.

[0172] (2) Feeding method of feed composition

[0173] Red sea bream fry of about 5 cm in size were used, and were fed twice a day in the morning and evening at a water temperature of 20 degrees ± 1.5°C, with the start of feeding as day 0, and were raised for 28 days. It should be noted that the red sea bream fry fed were ingested without problems when any of the feed compositions of the present invention was used. In addition, it was confirmed that the red sea bream fry that ingested the feed composition of the present invention could grow without discomfort such as diarrhea.

[0174] (3) Determination of weight gain rate

[0175] The weight of the fish at 0 day and 28 day was measured, and the value calculated by the following formula was defined as the weight increase rate.

[0176] Weight gain rate

[0177] ={(weight at 28 days) / (weight at 0 days)×100}-100

[0178] [Table 3]

[0179]

[0180] ※1: Mass parts per 100 parts by mass of protein source (mixture of soybean meal and fish meal)

[0181] ※2: Content based on 100 parts by mass of component (C) (net protein content) contained in the protein source

[0182] ※3: Relative value when Comparative Example 3-n is set to 100 (n=1 to 5).

Claims

1. A feed additive composition comprising: (A) anionic surfactant, hereinafter referred to as component (A); and (B) a nonionic surfactant, hereinafter referred to as component (B), The mass ratio of the content of the component (A) to the content of the component (B), that is, (A) / (B) is 0.7 or more and 20 or less.

2. The feed additive composition according to claim 1, wherein The component (A) is an anionic surfactant selected from the group consisting of alkyl sulfates, alkylbenzene sulfonates, alkyl sulfosuccinates and bile salts.

3. The feed additive composition according to claim 1 or 2, wherein The HLB of the component (B) is 6 or more and 18 or less.

4. A feed composition comprising: (A) anionic surfactant, hereinafter referred to as component (A); (B) a nonionic surfactant, hereinafter referred to as component (B); and (C) protein, hereinafter referred to as component (C), The mass ratio of the content of the component (A) to the content of the component (B), that is, (A) / (B) is 0.7 or more and 20 or less.

5. The feed composition according to claim 4, wherein The component (A) is an anionic surfactant selected from the group consisting of alkyl sulfates, alkylbenzene sulfonates, alkyl sulfosuccinates and bile salts.

6. The feed composition according to claim 4 or 5, wherein The (A) component is contained in an amount of 0.1 parts by mass or more and 50 parts by mass or less based on 100 parts by mass of the (C) component.

7. The feed composition according to any one of claims 4 to 6, wherein The HLB of the component (B) is 6 or more and 18 or less.

8. The feed composition according to any one of claims 4 to 7, wherein The (B) component is contained in an amount of 0.1 parts by mass or more and 20 parts by mass or less based on 100 parts by mass of the (C) component.

9. The feed composition according to any one of claims 4 to 8, wherein (C) Ingredient contains plant protein. 10 . The feed composition according to claim 4 , which is a feed for farming fish and shellfish.

11. A protein digestion promoter composition for fish farming, comprising: (A) anionic surfactant, hereinafter referred to as component (A); (B) a nonionic surfactant, hereinafter referred to as component (B); and (C) protein, hereinafter referred to as component (C), The mass ratio of the content of the component (A) to the content of the component (B), that is, (A) / (B) is 0.7 or more and 20 or less.

12. The protein digestion promoting agent composition for fish farming according to claim 11, wherein: The component (A) is an anionic surfactant selected from the group consisting of alkyl sulfates, alkylbenzene sulfonates, alkyl sulfosuccinates and bile salts.

13. The protein digestion promoting agent composition for fish farming according to claim 11 or 12, wherein: The HLB of the component (B) is 6 or more and 18 or less.

14. A method for producing a feed composition, comprising mixing component (A), component (B) and component (C) so that the mass ratio (A) / (B) of the content of component (A) to the content of component (B) is in the range of 0.7 or more and 20 or less, wherein component (A) is (A) anionic surfactant, component (B) is (B) nonionic surfactant, and component (C) is (C) protein.

15. A method for cultivating fish and shellfish, comprising feeding a feed composition containing component (A), component (B) and component (C) in a range where the mass ratio of the content of component (A) to the content of component (B), i.e., (A) / (B), is 0.7 or more and 20 or less, to the cultivated fish and shellfish, wherein component (A) is (A) anionic surfactant, component (B) is (B) nonionic surfactant, and component (C) is (C) protein.

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