Method for comprehensively preventing and treating black rot of cauliflower containing biological agent

Abstract

The invention discloses a biological agent-containing cauliflower black rot comprehensive prevention and control method, which comprises the following steps: S1, transplanting treatment: carrying out root irrigation treatment on seedlings after the cauliflower is transplanted by using a mixed agent, 200-500ml of the mixed agent is used for each cauliflower; the mixed bacterial agent comprises bacterial suspensions of Penicillium restrictum and Paecilomyces lilacinus, wherein the effective viable count of the Penicillium restrictum and the Paecilomyces lilacinus is not less than 1 * 10 < 8 > CFU / mL, and the volume ratio of the bacterial suspensions of the Penicillium restrictum and the Paecilomyces lilacinus is (1-3): 1; s2, spraying treatment, wherein after the cauliflower seedlings survive after being transplanted, the plants are sprayed with a complex microbial inoculant; the complex microbial inoculant comprises a bacterial suspension of penicillium restrictum and paecilomyces lilacinus in a volume ratio of (4-6): 1; the effective viable count of the bacterial suspension of the penicillium restrictum and the bacterial suspension of the paecilomyces lilacinus is not less than 1 * 10 < 8 > CFU / mL. By means of the method, the black rot morbidity of the cauliflower from the transplanting period to the survival period, from the survival period to the heading period and from the heading period to the picking period can be effectively reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of vegetable disease prevention and control, and specifically relates to a comprehensive prevention and control method for cauliflower black rot containing a biological bacterial agent. Background Art

[0002] Cauliflower (Brassica oleracea var.botrytis L.), also known as cauliflower and cauliflower, is a variety of Brassica oleracea in the cruciferous family that produces cauliflowers. It originated from Crete in the eastern Mediterranean and was introduced to my country from Britain in the middle of the century. As one of the most important vegetable species in the world, cauliflower is rich in nutrition, tender in texture, delicious in taste, high in water content and low in calories. It can fill the stomach without making you fat, and is becoming more and more popular among consumers.

[0003] Cauliflower is popular in the market, and the planting area is gradually increasing. However, with the increase in planting area, the incidence of black rot is also increasing. Black rot is a bacterial disease, and its pathogen is Xanthomonas brassicae, a pathogenic subspecies of brassicae. Black rot can occur during the transplanting, heading and seeding stages of cauliflower, and the disease cycle is long. Especially during the transplanting and heading stages, it will cause plant deformation, inability to grow and develop normally, and even death, which is very harmful. Summary of the invention

[0004] In order to overcome the problems existing in the prior art, the present invention proposes a comprehensive prevention and control method for cauliflower black rot containing a biological bacterial agent.

[0005] To achieve the above object, the present invention is implemented through the following technical solutions:

[0006] A comprehensive control method for cauliflower black rot containing a biological bacterial agent comprises the following steps:

[0007] S1, transplanting treatment: use a mixed bacterial agent to irrigate the roots of the transplanted cauliflower seedlings, 200m-500ml per cauliflower plant; the mixed bacterial agent contains an effective live bacterial count of not less than 1×10 8 CFU / mL of Penicillium restrictionum and Paecilomyces lilacinus suspensions, with the volume ratio of the two suspensions being 1-3:1;

[0008] S2, spraying treatment: after transplanting the cauliflower seedlings, the plants are sprayed with a composite bacterial agent; the composite bacterial agent contains a suspension of Penicillium restrictum and Paecilomyces lilacinus at a volume ratio of 4-6:1; the number of effective live bacteria in the suspension of Penicillium restrictum and Paecilomyces lilacinus is not less than 1×10 8 CFU / mL.

[0009] Furthermore, the preparation method of the restricted Penicillium suspension is: inoculating the restricted Penicillium test tube strain into a PDB liquid culture medium, and culturing at 26°C-28°C and 180-200r / min to obtain a liquid strain; the PDB liquid culture medium contains: 200 grams of potatoes, 20 grams of grape syrup, 1000 milliliters of water, and the culture medium pH = 6.0-6.5.

[0010] Furthermore, the preparation method of the Paecilomyces lilacinus suspension is as follows: inoculating the test tube strain of Paecilomyces lilacinus into a PDB liquid fermentation medium, and culturing at 25-28°C and 180-200r / min to obtain a liquid strain; the PDB liquid fermentation medium contains 200g of potato, 20g of glucose, and KH 2 PO 4 3g, MgS0 4 7H 2 01.5g, Vitanib B1Trace 8mg, water 1L, culture medium at natural pH.

[0011] Through the above technical scheme, the present invention can at least achieve the following beneficial effects: the method described in the present invention can effectively reduce the incidence of black rot of cauliflower from transplanting to survival period, from survival to heading period, and from heading to harvesting period. DETAILED DESCRIPTION

[0012] Unless otherwise specified, the materials and reagents used in the present invention are commercially available.

[0013] The cauliflower seeds used in the present invention are "Shengsong 718" cauliflower seeds produced by Tianjin Gengyun Seed Co., Ltd.

[0014] The Penicillium restrictum in the present invention was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., and the strain number is: Bio-09873.

[0015] The Paecilomyces lilacinus in the present invention was purchased from Beijing Biobo Biotechnology Co., Ltd., and the strain number is Bio-52225.

[0016] The preparation method of the restricted Penicillium suspension is as follows: inoculate the purchased restricted Penicillium test tube strain into PDB liquid culture medium (200 grams of potato, 20 grams of grape seed, 1000 milliliters of water; pH = 6.0-6.5), 26°C-28°C, 180-200r / min, and culture to obtain liquid strains.

[0017] The preparation method of the suspension of Paecilomyces lilacinus is as follows: inoculate the test tube strain of Paecilomyces lilacinus into PDB liquid fermentation medium (200 g potato, 20 g glucose, KH 2 PO 4 3g, MgS0 4 7H 2 01.5g, Vitanib B1 (thiamine) Trace 8mg, water 1L, natural pH), 25-28℃, 180-200r / min to obtain liquid strain.

[0018] Embodiment 1

[0019] A comprehensive control method for cauliflower black rot containing a biological bacterial agent comprises the following steps:

[0020] S1, transplanting treatment: Use mixed bacterial agent to irrigate the roots of transplanted cauliflower seedlings, 300 ml per cauliflower plant; the mixed bacterial agent contains about 1×10 8 CFU / mL of Penicillium restrictum and Paecilomyces lilacinus suspensions, with the volume ratio of the two suspensions being 2:1;

[0021] S2, spraying treatment: after cauliflower seedlings survive after transplantation, the plants are sprayed with a composite bacterial agent. During the period from transplantation survival to heading, spray once every 10 days. From the beginning of heading, spray once every 7 days and end spraying 7 days before picking. The composite bacterial agent contains a suspension of Penicillium restrictedum and Paecilomyces lilacinus in a volume ratio of 5:1; the effective viable bacterial counts of the suspensions of Penicillium restrictedum and Paecilomyces lilacinus are both about 1×10 8 CFU / mL.

[0022] Embodiment 2

[0023] A comprehensive control method for cauliflower black rot containing a biological bacterial agent comprises the following steps:

[0024] S1, transplanting treatment: The mixed bacterial agent was used to irrigate the roots of the transplanted cauliflower seedlings, 200 m per cauliflower plant; the mixed bacterial agent contained an effective number of live bacteria of about 1×10 8 CFU / mL of Penicillium restrictum and Paecilomyces lilacinus suspensions, with the volume ratio of the two suspensions being 1:1;

[0025] S2, spraying treatment: after cauliflower seedlings are transplanted and survived, the plants are sprayed with a composite bacterial agent. During the period from transplanting survival to heading, the plants are sprayed once every 10 days. From the beginning of heading, the plants are sprayed once every 7 days and the spraying is stopped 7 days before picking. The composite bacterial agent contains a suspension of Penicillium restrictum and Paecilomyces lilacinus in a volume ratio of 4:1. The number of effective live bacteria in the suspension of Penicillium restrictum and Paecilomyces lilacinus is about 1×10 8 CFU / mL.

[0026] Embodiment 3

[0027] A comprehensive control method for cauliflower black rot containing a biological bacterial agent comprises the following steps:

[0028] S1, transplanting treatment: Use mixed bacterial agent to irrigate the roots of cauliflower seedlings after transplanting, 500 ml per cauliflower plant; the mixed bacterial agent contains about 1×10 8 CFU / mL of Penicillium restrictum and Paecilomyces lilacinus suspensions, with the volume ratio of the two suspensions being 3:1;

[0029] S2, spraying treatment: after the cauliflower seedlings survive after transplantation, the plants are sprayed with a composite bacterial agent. During the period from transplantation survival to heading, the plants are sprayed once every 10 days. From the beginning of heading, the plants are sprayed once every 7 days, and the spraying is stopped 7 days before picking. The composite bacterial agent contains a suspension of Penicillium restrictedum and Paecilomyces lilacinus in a volume ratio of 6:1. The number of effective live bacteria in the suspension of Penicillium restrictedum and Paecilomyces lilacinus is about 1×10 8 CFU / mL.

[0030] Comparative Example 1

[0031] The difference between Comparative Example 1 and Example 1 is that in the mixed bacterial agent, the bacterial suspension of Penicillium restrictum is replaced with the bacterial suspension of Paecilomyces lilacinus.

[0032] Comparative Example 2

[0033] The difference between Comparative Example 2 and Example 1 is that in the mixed bacterial agent, the bacterial suspension of Paecilomyces lilacinus is replaced with a bacterial suspension of Penicillium restrictum.

[0034] Comparative Example 3

[0035] The difference between Comparative Example 3 and Example 1 is that in the composite bacterial agent, the suspension of Penicillium restrictum is replaced with the suspension of Paecilomyces lilacinus.

[0036] Comparative Example 4

[0037] The difference between Comparative Example 4 and Example 1 is that in the composite bacterial agent, the bacterial suspension of Paecilomyces lilacinus is replaced with a bacterial suspension of Penicillium restrictum.

[0038] Experimental example

[0039] The experiment was designed with control group one and control group two;

[0040] The difference between the control group 1 and the example 1 is that only step S1 is performed, and the mixed bacterial agent is replaced with the same volume of sterile water;

[0041] The difference between the control group 2 and the example 1 is that in step S2, the composite bacterial agent is replaced with the same volume of sterile water;

[0042] During the experiment:

[0043] In each embodiment, comparative example and control group 1 and 2, 200 cauliflower plants with consistent growth and no obvious diseases were selected for transplanting and root irrigation treatment. The root irrigation treatment included root irrigation of the cauliflower plants with mixed bacterial agents for 3 days after transplanting, and then root irrigation with effective live bacteria count of 1×10 8 CFU / mL of Xanthomonas brassicae subspecies brassicae bacterial suspension 200ml. Seven days after root irrigation with pathogenic bacteria, the incidence of black rot in cauliflower plants was counted and recorded as the incidence of black rot from transplanting to survival stage;

[0044] After counting the incidence of black rot in the transplanted cauliflower plants to the survival period, 100 cauliflower plants with uniform growth and no obvious diseases were selected for step S2, and the composite bacterial agent was sprayed according to the recorded technical scheme, and the front and back sides of the leaves and stems of the plants were sprayed in place during the spraying;

[0045] The period from the completion of transplanting to the black rot incidence during the survival period to the first cauliflower plant forming a head is the survival to heading period;

[0046] After the incidence of black rot in the head-forming stage was counted, 50 cauliflower plants with uniform growth and no obvious diseases were selected and sprayed with the composite bacterial agent according to the recorded technical plan. During the spraying, the front and back sides of the leaves, stems, and flower heads of the plants were sprayed in place.

[0047] The period from the first cauliflower head to the time when it is ripe and ready for picking is called the head-to-picking period.

[0048] Incidence rate = diseased plants / total number of plants*100%

[0049] The statistical results are shown in Table 1 below:

[0050] Table 1 Statistics of the incidence of cauliflower black rot

[0051]

[0052] As can be seen from Table 1 above, using a mixed bacterial agent containing Penicillium restrictum and Paecilomyces lilacinus to irrigate the roots of cauliflower plants and using a composite bacterial agent containing Penicillium restrictum and Paecilomyces lilacinus to spray the cauliflower plants can significantly reduce the incidence of black rot in cauliflower plants from transplanting to survival period, survival to heading period, and heading to harvesting period.

[0053] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined by the claims of the present invention.

Claims

1. A method for comprehensive prevention and control of cauliflower black rot containing a biological agent, characterized in that: The following steps are involved: S1, transplanting treatment: use a mixed bacterial agent to irrigate the roots of the transplanted cauliflower seedlings, 200m-500ml per cauliflower plant; the mixed bacterial agent contains an effective live bacterial count of not less than 1×10 8 CFU / mL of Penicillium restrictionum and Paecilomyces lilacinus suspensions, with the volume ratio of the two suspensions being 1-3:1; S2, spraying treatment: after transplanting the cauliflower seedlings, the plants are sprayed with a composite bacterial agent; the composite bacterial agent contains a suspension of Penicillium restrictum and Paecilomyces lilacinus at a volume ratio of 4-6:1; the number of effective live bacteria in the suspension of Penicillium restrictum and Paecilomyces lilacinus is not less than 1×10 8 CFU / mL.

2. The method for comprehensive prevention and control of cauliflower black rot containing a biological agent according to claim 1, characterized in that: The preparation method of the restricted Penicillium suspension is as follows: inoculating the restricted Penicillium test tube strain into a PDB liquid culture medium, and culturing the liquid strain under the conditions of 26°C-28°C and 180-200r / min; the PDB liquid culture medium comprises: 200 grams of potato, 20 grams of grape syrup, 1000 milliliters of water, and the culture medium pH=6.0-6.

5.

3. The method for comprehensive prevention and control of cauliflower black rot containing a biological agent according to claim 1, characterized in that: The preparation method of the Paecilomyces lilacinus suspension is as follows: inoculating a test tube strain of Paecilomyces lilacinus into a PDB liquid fermentation medium, and culturing at 25-28° C. and 180-200 r / min to obtain a liquid strain; the PDB liquid fermentation medium contains 200 g of potato, 20 g of glucose, 3 g of KH2PO4, 1.5 g of MgS04·7H20, 8 mg of Vitanib B1 Trace, and 1 L of water, and the medium has a natural pH.

Images