Liquid nitrogen container equipped with a quick cap - mounting mechanical device for a live - load pole
Through the design of the fast-loading mechanical device for loading rods, the problem of positioning rods and rod caps in liquid nitrogen environment is solved, and efficient and safe loading rods and rod caps are achieved, reducing the operating risk and the probability of embryo/egg loss.
Patent Information
- Application Number
- CN202510497440.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-21
- Publication Date
- 2025-07-22
- Estimated Expiration
- 2045-04-21
AI Technical Summary
When operators perform closed operations of the load rod and rod cap in liquid nitrogen environment, they are easily affected by liquid nitrogen mist, liquid surface fluctuations and vision differences, resulting in repeated operations increasing the risk of embryo/egg loss. Long-term exposure to liquid nitrogen environment is harmful to health. The existing automatic rod cover device cannot closely realize the positioning of the load rod and rod cap.
The fast-loading mechanism of the load rod is adopted, including the load rod ladder conveying mechanism and the rod cap sleeve. Through the coordination of the transmission rail, propulsion rod, crank rod and transmission disc, the precise positioning and quick connection between the load rod and the rod cap is achieved. The design of the transmission mechanism and the cap assembly is used to ensure the accurate butt and fixation of the load rod and the rod cap.
It improves the success rate of load rod and rod cap sockets, reduces the risk of embryo/egg loss, reduces the health risks of operators, and achieves efficient and safe operation in liquid nitrogen environment.
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Figure CN120021614B_ABST
Abstract
Description
Technical Field
[0001] This application relates to the technical field of manipulators or containers equipped with operating devices, and particularly to a liquid nitrogen container equipped with a rapid cap-fitting mechanical device for a loading rod. Background Art
[0002] Currently, there are two methods for embryo / egg cryopreservation: programmed cryopreservation and vitrification cryopreservation. The main technical route of vitrification cryopreservation is to balance the embryo / egg with a high-concentration cryoprotectant for a certain period of time, reduce the cell volume by dehydration, and then place it on a freezing rod and directly immerse it in liquid nitrogen to instantaneously vitrify the cells, so as to reduce the damage caused by intracellular ice formation. The last and important step of the vitrification cryopreservation technology is: under liquid nitrogen, perform a cap-fitting and sealing operation on the freezing rod with the embryo / egg placed on it.
[0003] However, in the above-mentioned rod-sleeving operation process, the following problems exist: When the operator caps and seals the thin and transparent slide under liquid nitrogen, it is easily affected by factors such as liquid nitrogen mist, liquid nitrogen liquid level fluctuation, and individual vision differences, resulting in repeated cap-fitting operations. And every time a cap-fitting operation is added, the risk of embryo / egg loss increases by one point; at the same time, staying in the liquid nitrogen environment for a long time will increase the risk of frostbite on the operator's fingers and excessive inhalation of nitrogen, which will affect physical health.
[0004] For this reason, Chinese Utility Model Patent CN2023203962084 discloses a new type of automatic rod-sleeving device for embryo cryopreservation rods. This automatic rod-sleeving device realizes automatic rod-sleeving through the action of gravity. However, since the frozen embryo / egg tries to minimize the carrying of excess liquid, and the reduction in volume can cause the freezing temperature to drop rapidly, crossing the dangerous period of ice crystal formation, the width of the transparent slide end is only about 1 mm. Relying solely on the weight of the rod itself to achieve the rod-sleeving operation, it is obviously impossible to tightly achieve the sleeving between the rod and the rod cap. Summary of the Invention
[0005] The present invention provides a liquid nitrogen container equipped with a rapid cap-fitting mechanical device for a loading rod, which has the advantages of assisting the operator to position between the rod and the rod cap, and being able to quickly process batch sample loading rods.
[0006] The object of the present invention is achieved through the following technical solutions:
[0007] A liquid nitrogen container equipped with a mechanical device for quickly fitting caps onto loading rods, comprising a loading rod stepped transmission mechanism and a rod cap sleeve; the loading rod stepped transmission mechanism includes a transmission rail, a propulsion rod, a crank lever, and a transmission disk; the transmission rail is provided with an out-position chute adapted to the sliding of the loading rod, one end of the propulsion rod is in transmission connection with the transmission disk, and the other end is in transmission connection with the loading rod for propulsion; the transmission disk is provided with a cam chute, one end of the crank lever is slidably disposed in the cam chute, and the other end is in transmission connection with the loading rod on the out-position chute, and the crank lever is rotatably mounted on the transmission rail; the rod cap sleeve is located below the loading rod.
[0008] Among them, the advantage of the loading rod stepped transmission mechanism is that after arranging the loading rods from top to bottom, they can be sequentially pushed above the rod cap sleeve. The operator uses tweezers to push the loading rod downward to fit it with the rod cap sleeve, and then pushes the loaded rod out of the out-position chute. The loaded rod is fixedly arranged in the rod cap sleeve, and the operation of sleeving the rod can be completed.
[0009] Preferably, the loading rod stepped transmission mechanism further includes a transmission convex portion, and a through groove adapted to the movement path of the transmission convex portion is provided at one end of the propulsion rod facing away from the loading rod.
[0010] Preferably, the loading rod stepped transmission mechanism further includes a transmission wheel, a connecting rod, and a rotating handle; the transmission wheel is in meshing transmission connection with the transmission disk, one end of the connecting rod is fixed to the transmission wheel, and the other end is fixed to the rotating handle.
[0011] Preferably, the loading rod stepped transmission mechanism further includes a cap-fitting assembly, the cap-fitting assembly includes a slide rail plate, a spring, and a slider, the slide rail plate is fixed to the transmission rail and is located at the end of the out-position chute, the slider is slidably disposed on the slide rail plate, the loading rod is clamped between the two slide rail plates, and one end of the spring is fixed to the bottom end of the slide rail plate and the other end is fixed to the slider.
[0012] Among them, the advantage of the cap-fitting assembly is that it completes the positioning between the loading rod and the rod cap, and can adapt to the operator to press down the loading rod to realize the process of sleeving the loading rod with the cap.
[0013] Preferably, a carry-in chute is provided on the transmission rail, the carry-in chute communicates with the out-position chute, and the propulsion rod is located at the connection of the carry-in chute and the out-position chute.
[0014] Preferably, a guide tube for guiding the loading rod to be inserted is correspondingly installed at the upper end of the carry-in chute.
[0015] Preferably, the liquid nitrogen container further includes a receiving housing, an outer housing, and a fixing frame;
[0016] The accommodation housing and the outer housing are rotationally sleeved; the fixing frame includes a connecting rod, a connecting disk, a fixing ring and a support rod; the rod caps are arranged in a circumferential array and fixed on the fixing ring, one end of the connecting rod is fixed to the fixing ring, and the other end is fixed to the connecting disk. The connecting disk and the bottom of the accommodation housing are fixed by a shaft, one end of the support rod is fixed to the connecting disk, and the other end is rotatably connected to the transmission rail.
[0017] Preferably, a feed inlet and an observation port are provided at the upper end of the outer housing, and both the feed inlet and the observation port are provided with sealing covers. The observation port is located above the slide rail plate.
[0018] Preferably, an indicating extension part is provided at the bottom side end of the accommodation housing, and indicating scales are provided on the indicating extension part. The orientation of the indicating scales is consistent with that of the rod cap sleeves.
[0019] Preferably, a rod-carrying indicating convex part is provided on the side wall of the outer housing.
[0020] Compared with the prior art, the advantages or beneficial effects of the technical solution of the present application include:
[0021] 1. By sequentially pushing the rod carriers along the out-position chute so that they are advanced to a position above the rod cap sleeves at the end, the alignment of the rod carriers and the rod caps placed on the rod cap sleeves is achieved in the vertical direction. When the operator drives the rod carriers downward towards the rod caps, the rod carriers and the rod caps can be sleeved and pressed against each other, thereby realizing the tight fixing of the two.
[0022] 2. Since the orientation of the rod carriers is pre-arranged, after driving them above the rod caps, the success rate of one-time tight fitting is high, avoiding the loss of eggs during the operation of loading the rods and closing. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 Schematic structural diagram of a liquid nitrogen container equipped with a rod carrier quick capping mechanical device;
[0024] Figure 2 Schematic cross-sectional structure diagram of a liquid nitrogen container;
[0025] Figure 3 Schematic three-dimensional structure diagram of a rod carrier ladder transmission mechanism;
[0026] Figure 4 For Figure 3 Enlarged view of part A in
[0027] Figure 5 For Figure 3 Enlarged view of part B in
[0028] Figure 6 Top view of the rod carrier ladder transmission mechanism;
[0029] Figure 7It is the motion state diagram of the carrier rod ladder transmission mechanism (the crank toggle rod and the carrier rod are not in contact, and the propulsion rod pushes the carrier rod forward);
[0030] Figure 8 It is the motion state diagram of the carrier rod ladder transmission mechanism (the crank toggle rod and the carrier rod are in contact, the propulsion rod retracts, and the next carrier rod falls in);
[0031] Figure 9 It is the schematic diagram of the matching operation of the carrier rod and the rod cap in Embodiment 3 (transparent acrylic is installed);
[0032] Figure 10 It is the schematic diagram of the matching operation of the carrier rod and the rod cap in Embodiment 4.
[0033] In the figure: 1. Rotating handle, 2. Connecting rod, 3. Transmission wheel, 4. Rod cap sleeve, 6. Guide tube, 7. Connecting plate, 8. Link rod, 9. Transmission disc, 10. Propulsion rod, 11. Transmission convex part, 12. Cam chute, 13. Support rod, 14. Transmission rail, 15. Crank toggle rod, 16. Carrier rod, 17. Slide block, 18. Elastic spring, 19. Slide rail plate, 20. Fixed ring, 23. Shaft, 24. Indicator extension part, 25. Indicator scale, 26. Carrier rod indicator convex part, 30. Outer shell, 31. Accommodating shell, 32. Rotating toggle rod, 33. Push rod, Specific implementation mode
[0034] The following will combine the drawings and embodiments to detail the implementation mode of the present application, so as to fully understand how the present application uses technical means to solve technical problems and the implementation process of achieving corresponding technical effects and implement accordingly. Each feature in the embodiments of the present application and the embodiments can be combined with each other on the premise of not conflicting, and the formed technical solutions are all within the protection scope of the present application.
[0035] It should be clear that the following described embodiments are only part of the embodiments of the present application, rather than all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments in the present application without creative work belong to the protection scope of the present application.
[0036] Embodiment 1: As Figures 1 - 6As shown in the figure, a liquid nitrogen container equipped with a quick cap - mounting mechanical device for the loading rod includes a stepped transmission mechanism for the rod and a rod - cap sleeve 4. The stepped transmission mechanism for the rod includes a transmission rail 14, a push rod 10, a crank - lever 15, a transmission disk 9, a transmission convex part 11, a transmission wheel 3, a connecting rod 2, and a rotating handle 1. The transmission rail 14 is provided with an out - position chute adapted to the sliding of the loading rod 16. One end of the push rod 10 is in transmission connection with the transmission disk 9, and the other end is in transmission connection with the loading rod 16 for pushing. The transmission disk 9 is provided with a cam chute 12. One end of the crank - lever 15 is slidably arranged in the cam chute 12, and the other end is in transmission connection with the loading rod 16 on the out - position chute. The crank - lever 15 is rotatably installed on the transmission rail 14. The rod - cap sleeve 4 is located below the loading rod 16. One end of the push rod 10 facing away from the loading rod 16 is provided with a through - groove adapted to the movement path of the transmission convex part 11. The transmission wheel 3 and the transmission disk 9 are in meshing transmission connection. One end of the connecting rod 2 is fixed to the transmission wheel 3, and the other end is fixed to the rotating handle 1.
[0037] The transmission rail 14 is provided with an in - position chute. The in - position chute and the out - position chute are connected through. The push rod 10 is located at the connection of the in - position chute and the out - position chute. A guide tube 6 for guiding the loading rod 16 to be inserted is correspondingly installed at the upper end of the in - position chute.
[0038] The connection relationship and working principle among the internal components of the stepped transmission mechanism for the rod are as follows:
[0039] When the loading rod 16 enters the in - position chute along the guide tube 6, the number of loading rods that each liquid nitrogen tank can hold is fixed. As Figure 3 shown, 30 loading rods can be loaded, and the corresponding rod - cap sleeves are 30. When the operator inserts the loading rod along the opening of the liquid nitrogen tank and then rotates the rotating handle 1, the transmission disk 9 can be driven to rotate by the transmission wheel 3.
[0040] Since the cam chute 12 is composed of a large arc and a small arc spliced together. When the end of the crank - lever 15 moves to the large arc, the other end of the crank - lever 15 does not contact the loading rod 16. On the contrary, when the end of the crank - lever 15 moves to the small arc, the other end of the crank - lever 15 contacts the loading rod 16, so that the loading rod 16 located on the in - position chute falls into the out - position chute (i.e., above the end of the push rod 10). During the above process, when the transmission convex part 11 moves to the left ( Figure 4 ), it will drive the end of the push rod 10 to push forward, thus pushing the loading rod 16 forward. On the contrary, when it moves to the right ( Figure 4 ), the push rod 10 will return to its original position. See the state display in Figures 7 - 8 for details.
[0041] In the above process, the sliding force of the carrier rod 16 on the carry chute is mainly its own gravity. Therefore, the part of the transmission rail 14 corresponding to the carry chute can be designed as an inclined plane, which is conducive to the carrier rod overcoming its own gravity and the buoyancy of liquid nitrogen, so that it can actively fall onto the out-position chute. Moreover, although the carrier rod 16 can rely on the push of the previous carrier rod 16 on the out-position chute, in order to avoid insufficient power, the part of the transmission rail 14 corresponding to the out-position chute can be designed as an inclined plane, so that the carrier rod 16 can slide along the out-position chute according to its own gravity until it slides between the two sliders 17.
[0042] Embodiment 2: On the basis of Embodiment 1, the structure of the carrier rod stepped transmission mechanism is further refined as follows:
[0043] As Figure 3 and Figure 5 shown, the carrier rod stepped transmission mechanism further includes a cap assembly. The cap assembly includes a slide rail plate 19, a tension spring 18 and a slider 17. The slide rail plate 19 is fixed to the transmission rail 14 and is located at the end of the out-position chute. The slider 17 is slidably arranged on the slide rail plate 19. The carrier rod 16 is clamped between the two slide rail plates 19. One end of the tension spring 18 is fixed to the bottom end of the slide rail plate 19, and the other end is fixed to the slider 17.
[0044] The component composition relationship and working principle of the cap assembly are as follows:
[0045] When the carrier rod 16 is stepped and pushed between the two slide rail plates 19, the operator can push the carrier rod towards the direction of the rod cap with tweezers to make them tightly sleeved. After the carrier rod 16 and the rod cap are tightly sleeved, the operator can continue to push forward, so as to push the carrier rod 16 out from between the two slide rail plates 19 (the bottom end of the rod cap is still in the rod cap sleeve 4, so after the carrier rod 16 is tightly sleeved, it can be placed on the rod cap sleeve 4). After being pushed out, under the action of the tension spring 18, the slider 17 can return to its original position and continue to wait for the next carrier rod 16 to perform the sleeve rod operation.
[0046] In the above process, the operator can observe through the observation port whether the carrier rod 16 is pushed between the two slide rail plates 19 to assist in positioning.
[0047] This embodiment further includes a receiving housing 31, an outer housing 30 and a fixing frame; the receiving housing 31 and the outer housing 30 are rotatably sleeved; the fixing frame includes a connecting rod 8, a connecting disk 7, a fixing ring 20 and a support rod 13; the rod cap sleeves 4 are arranged in a circular array and fixed on the fixing ring 20. One end of the connecting rod 8 is fixed to the fixing ring 20, and the other end is fixed to the connecting disk 7. The connecting disk 7 and the bottom of the receiving housing 31 are fixed by a shaft 23. One end of the support rod 13 is fixed to the connecting disk 7, and the other end is rotatably connected to the transmission rail 14.
[0048] In this embodiment, a feed inlet and an observation port are provided at the upper end of the outer casing 30. Both the feed inlet and the observation port are equipped with sealing caps, and the observation port is located above the slide rail plate 19.
[0049] In this embodiment, an indicating extension portion 24 is provided at the bottom side end of the accommodation housing 31, and an indicating scale 25 is provided on the indicating extension portion 24. The orientation of the indicating scale 25 is consistent with that of the rod cap sleeve 4.
[0050] In this embodiment, a rod-carrying indicating convex portion 26 is provided on the side wall of the outer casing 30.
[0051] When performing the positioning operation, the indicating extension portion 24 can be rotated so that the indicating scale 25 corresponding to the rod cap sleeve 4 corresponds to the rod-carrying indicating convex portion 26 on the outer casing 30, which indicates that the rod 16 has been aligned with the next rod cap (the rod 16 and the rod-carrying indicating convex portion 26 are aligned in advance).
[0052] Embodiment 3: This embodiment provides a method for the rod-carrying cap that is different from Embodiments 1-2:
[0053] As Figure 9 shown, by providing transparent acrylic at the observation port, the lower end of the transparent acrylic is located below the liquid nitrogen level and directly above the rod 16. The operator can observe the position of the rod 16 below the liquid nitrogen through the transparent acrylic, avoiding the interference of the liquid nitrogen mist, and the practical effect is very good. At the same time, a gap can be left between the transparent acrylic and the rod 16 to accommodate the operator to insert a metal rod and push the upper end of the rod 16 downward, thus completing the entire cap-fitting process.
[0054] Moreover, in this embodiment, in order to prevent the rod 16 from falling into the liquid nitrogen when moving in the out-position chute, a limiting piece is rotatably connected to the slider 17 (the limiting piece can be reset by a torsion spring), so that through the limitation of the limiting piece, the rod 16 can be prevented from accidentally moving and falling into the liquid nitrogen by itself (it can prevent the rod 16 from falling when sliding along the inclined out-position chute).
[0055] Embodiment 4: On the basis of Embodiment 3, this embodiment further proposes a method for pushing the rod 16 into the rod cap that is different from the former, specifically:
[0056] As Figure 10 shown, the transparent acrylic is installed at the observation port and aligned above the rod 16 for observing the position of the rod.
[0057] At the same time, a new operation window is added. Inside the operation window, a rotating lever 32 and a push rod 33 that can move in the vertical direction are rotatably installed. Both the rotating lever 32 and the push rod 33 are in an L shape.
[0058] The operation process is as follows: S1. Press down the push rod 33 (in the direction directly above the carrier rod 16), so that the carrier rod 16 moves downward until it is sleeved and fixed with the rod cap. S2. Rotate the rotating lever 32, so that the end of the rotating lever 32 pulls out the carrier rod 16 from the two sliders 17 and places it in the rod cap sleeve 4.
[0059] In the above process, when the rotating lever 32 rotates to pull out the carrier rod 16, it is considered that the carrier rod 16 itself has toughness to achieve this. And after the operation is completed, the push rod 33 returns to its original position due to the elastic force of the spring.
[0060] In the above process, although the end of the push rod 33 is located above the carrier rod 16, it does not affect the operator's observation of the overall situation of the carrier rod 16 through the transparent acrylic (that is, the situation where the end of the push rod 33 blocks the upper part of the carrier rod 16 is not considered).
Claims
1. A liquid nitrogen container installed with a mechanical device for quickly installing a cap on a live rod, characterized in that, It includes a carrier rod step transmission mechanism and a rod cap sleeve (4); The carrier rod step transmission mechanism includes a transmission rail (14), a propulsion rod (10), a crank lever (15) and a transmission disc (9); an out-position sliding groove adapted for the sliding of the carrier rod (16) is provided on the transmission rail (14), one end of the propulsion rod (10) is in transmission connection with the transmission disc (9), and the other end is in propulsion transmission with the carrier rod (16); a cam sliding groove (12) is provided on the transmission disc (9), one end of the crank lever (15) is slidably arranged in the cam sliding groove (12), and the other end is in transmission connection with the carrier rod (16) on the out-position sliding groove. The crank lever (15) is rotatably installed on the transmission rail (14); the rod cap sleeve (4) is located below the carrier rod (16); The carrier rod step transmission mechanism further includes a transmission convex part (11), a transmission wheel (3), a connecting rod (2) and a rotating handle (1). A through groove adapted for the movement path of the transmission convex part (11) is provided at one end of the propulsion rod (10) facing away from the carrier rod (16); the transmission wheel (3) is in meshing transmission connection with the transmission disc (9), one end of the connecting rod (2) is fixed to the transmission wheel (3), and the other end is fixed to the rotating handle (1); A carry-in sliding groove is provided on the transmission rail (14), the carry-in sliding groove communicates with the out-position sliding groove, and the propulsion rod (10) is located at the connection of the carry-in sliding groove and the out-position sliding groove; It further includes a receiving and accommodating shell (31), an outer shell (30) and a fixing frame; the fixing frame includes a connecting rod (8), a connecting disc (7), a fixing ring (20) and a support rod (13); the rod cap sleeves (4) are fixedly arranged in a circumferential arrangement on the fixing ring (20), one end of the connecting rod (8) is fixed to the fixing ring (20), the other end is fixed to the connecting disc (7), and one end of the support rod (13) is fixed to the connecting disc (7), and the other end is rotatably connected to the transmission rail (14).
2. The liquid nitrogen container equipped with the mechanical device for quickly installing caps on the load-bearing rods according to claim 1, characterized in that, The carrier rod step transmission mechanism further includes a cap-fitting assembly, and the cap-fitting assembly includes a slide rail plate (19), a elastic spring (18) and a slider (17). The slide rail plate (19) is fixed to the transmission rail (14) and is located at the end of the out-position sliding groove. The slider (17) is slidably arranged on the slide rail plate (19). The carrier rod (16) is clamped between two slide rail plates (19). One end of the elastic spring (18) is fixed to the bottom end of the slide rail plate (19), and the other end is fixed to the slider (17).
3. The liquid nitrogen container equipped with the mechanical device for quickly installing caps on the live poles according to claim 1, characterized in that, A guide tube (6) for guiding the placement of the carrier rod (16) is correspondingly installed at the upper end of the carry-in sliding groove.
4. The liquid nitrogen container equipped with the mechanical device for quickly installing a cap on a live pole according to claim 1, characterized in that, The receiving and accommodating shell (31) and the outer shell (30) are rotatably sleeved; the connecting disc (7) and the bottom of the receiving and accommodating shell (31) are fixed by a shaft (23).
5. The liquid nitrogen container equipped with the mechanical device for quickly installing a cap on a live rod according to claim 2, characterized in that, The upper end of the outer shell (30) is provided with a feeding port and an observation port, and both the feeding port and the observation port are provided with sealing covers. The observation port is located above the slide rail plate (19).
6. The liquid nitrogen container equipped with the mechanical device for quickly installing caps on the load-bearing rods according to claim 4, characterized in that, An indicating extension part (24) is provided at the bottom side end of the receiving and accommodating shell (31), and an indicating scale (25) is provided on the indicating extension part (24). The orientation of the indicating scale (25) is the same as that of the rod cap sleeve (4).
7. The liquid nitrogen container equipped with the mechanical device for quickly installing caps on the live poles according to claim 4, characterized in that, A carrier rod indicating convex part (26) is provided on the side wall of the outer shell (30).
Citation Information
Patent Citations
Auxiliary safe docking device of human embryo freezing carrier poles under liquid nitrogen and using method of auxiliary safe docking device
CN108496956A
Automated biological sample vitrification, storage and thawing system
CN118202186A