Application of longan peel essential oil in preparation of repairing and whitening product

Abstract

The invention provides application of longan peel essential oil in preparation of a repairing and whitening product, and an extraction method of the longan peel essential oil comprises the following steps: (1) cleaning longan peel, drying and crushing to obtain longan peel powder; and (2) adding the longan peel powder into deionized water, performing steam distillation extraction, collecting distillate, and performing normal-pressure condensation to obtain the longan peel essential oil. A product prepared from the extracted longan peel essential oil has good repairing and whitening effects. The extraction process of the longan peel essential oil is low in cost, simple in flow and easy to popularize on a large scale, fills the blank of processing and utilization of the longan peel at present, realizes high-value utilization of longan processing byproducts, and has a good development prospect.

Description

Technical Field

[0001] The invention relates to the technical field of skin care products, and in particular to application of longan peel essential oil in preparing repairing and whitening products. Background Art

[0002] Longan (Dimocarpus longan Lour.) is an evergreen tree of the Sapindaceae family. It is a famous subtropical fruit in my country with a cultivation history of more than 2,000 years. my country's longan production accounts for about 60% of the world's total. Its pulp is rich in dietary fiber, vitamins, polysaccharides and amino acids, etc. It has high nutritional value and the effects of appetizing, strengthening the spleen, calming the mind and replenishing qi. It is considered to be a "medicine and food of the same origin" and is often used in traditional Chinese medicine to treat insomnia, insufficient heart blood, physical weakness, spleen deficiency and diarrhea.

[0003] At present, in addition to fresh sales, longan can be processed into canned longan meat, dried longan and longan wine, etc., but there is almost no development and utilization of the processing byproduct peel, which accounts for 17% of the weight of fresh fruit. Longan peel contains many active ingredients, such as protocatechuic acid, gallic acid, corilagin, geraniol, quercetin, (-) epicatechin, etc. Therefore, it is of practical significance to extract and utilize the active ingredients in longan peel. Summary of the invention

[0004] In view of this, the present invention proposes the use of longan peel essential oil in the preparation of repairing and whitening products.

[0005] The technical solution of the present invention is achieved in this way:

[0006] Application of longan peel essential oil in the preparation of repairing and whitening products, the extraction method of the longan peel essential oil is:

[0007] (1) cleaning longan peel, drying, and crushing to obtain longan peel powder;

[0008] (2) Adding longan peel powder to deionized water, extracting by steam distillation, collecting the distillate, and condensing at normal pressure to obtain longan peel essential oil.

[0009] Furthermore, in step (2) of the method for extracting longan peel essential oil, the solid-liquid ratio of the longan peel powder to deionized water is 1:6-10 g / mL.

[0010] Furthermore, in step (2) of the method for extracting longan peel essential oil, the conditions for the steam distillation are specifically as follows: first, the temperature is raised to 160-180°C at a heating rate of 5°C / min and maintained for 10-15 minutes, and then the temperature is lowered to 110-130°C and maintained for 2-5 hours.

[0011] Furthermore, the repair and whitening product includes the following raw materials in percentage by mass: 0.1-0.3% longan peel essential oil, 1.5-4% 1,3-butylene glycol, 1-3% glycerin, 0.5-2% niacinamide, 0.3-0.6% 1,2-hexanediol, 0.4-1.2% erythritol, 0.3-1% D-panthenol, 0.3-1% lubric acid oil, 0.3-0.6% cinnamone, 0.5-1% tetrahydromethylpyrimidine carboxylic acid, 0.2-0.5% carbomer 980, 0.2-0.5% arginine, 0.1-0.5% sodium hyaluronate, 0.1-0.3% transdermal polypeptide, 0.02-0.05% Centella asiatica extract, 0.01-0.05% ethylhexylglycerin, and the rest is deionized water.

[0012] Furthermore, the total glycoside content in the Centella asiatica extract is 90%-95%; the molecular weight of the sodium hyaluronate is 130w-150w Da.

[0013] Furthermore, the preparation method of the repair and whitening product is specifically as follows:

[0014] (1) 0.4%-1.2% 1,3-butylene glycol, carbomer 980, sodium hyaluronate and glycerin are mixed and stirred to obtain phase A1;

[0015] Mix niacinamide, D-panthenol, lupinus root gum oil and erythritol to obtain phase A2;

[0016] Mix arginine and 2%-3% deionized water to obtain phase B;

[0017] Mix cinnamone and the remaining 1,3-butanediol and heat to 60°C, and stir at constant temperature until transparent to obtain phase C;

[0018] Add the Centella asiatica extract and tetrahydropyrimidine carboxylic acid into 2%-5% deionized water and stir evenly to obtain phase D;

[0019] Mix longan peel essential oil, transdermal peptide, 1,2-hexanediol and ethylhexylglycerin to obtain phase E;

[0020] (2) Add the remaining deionized water into the main pot, stir and heat to 80-90°C, add phase A2 and phase A1 in sequence, homogenize, stir at 80-90°C for 30-35 minutes, cool to 60°C, add phase B, phase C and phase D, continue stirring and cool to below 45°C, add phase E, stir evenly, and cool to below 38°C to obtain the target repair and whitening product.

[0021] Furthermore, step (2) of the preparation method of the repair and whitening product is specifically as follows: add the remaining deionized water into the main pot, stir and heat to 80-90°C, add phase A2 and phase A1 in sequence, homogenize at 3000HZ for 5 minutes, then stir at 80-90°C, 15-25HZ for 30-35 minutes, cool to 60°C, add phase B, phase C and phase D, continue stirring at 10-15HZ and cool to below 45°C, add phase E, stir evenly, cool to below 38°C, filter the material through 100 mesh to obtain the target repair and whitening product.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The extraction method of the invention does not destroy active ingredients, accelerates the extraction speed of longan peel essential oil, has a high essential oil rate and high safety, and the products prepared by the obtained longan peel essential oil have good repairing and whitening effects. The extraction process of the longan peel essential oil of the invention has low cost, simple process and is easy to promote on a large scale, and at the same time fills the gap in the current longan peel processing and utilization, realizes the high-value utilization of longan processing by-products, and has good development prospects. DETAILED DESCRIPTION

[0024] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0025] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0026] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0027] Example 1

[0028] A method for extracting longan peel essential oil, the specific steps comprising:

[0029] (1) washing longan peel, drying at a constant temperature of 60° C., and crushing to obtain longan peel powder;

[0030] (2) According to the solid-liquid ratio of 1:7 g / mL, longan peel powder was added to deionized water for steam distillation. The temperature was first raised to 170°C at a rate of 5°C / min and maintained for 13 min. The temperature was then lowered to 120°C and extracted for 4 h at normal pressure and a condensation temperature of 8°C. The distillate was collected to obtain longan peel essential oil.

[0031] A repairing and whitening product, comprising the following raw materials in terms of mass percentage:

[0032]

[0033]

[0034] The preparation method of the above-mentioned repair and whitening product is specifically as follows:

[0035] (1) 0.8% 1,3-butylene glycol, carbomer 980, sodium hyaluronate and glycerin are mixed and stirred to obtain phase A1;

[0036] Mix niacinamide, D-panthenol, lupinus root gum oil and erythritol to obtain phase A2;

[0037] Mix arginine and 3% deionized water to obtain phase B;

[0038] Mix cinnamic acid and 1.7% 1,3-butanediol, heat to 60°C, and stir at constant temperature until transparent to obtain phase C;

[0039] Add Centella asiatica extract and tetrahydropyrimidine carboxylic acid into 5% deionized water and stir well to obtain phase D;

[0040] Mix longan peel essential oil, transdermal peptide, 1,2-hexanediol and ethylhexylglycerin to obtain phase E;

[0041] (2) Add 81.33% deionized water to the main pot, stir and heat to 85°C, add phase A2 and phase A1 in sequence, homogenize at 3000HZ for 5 minutes, then stir at 85°C, 20HZ for 32 minutes, cool to 60°C, add phase B, phase C and phase D, continue stirring at 13HZ until the temperature drops to below 45°C, add phase E, stir evenly, cool to below 38°C, filter the material through 100 mesh to obtain a repairing and whitening product.

[0042] Example 2

[0043] A method for extracting longan peel essential oil, the specific steps comprising:

[0044] (1) washing longan peel, drying at a constant temperature of 55° C., and crushing to obtain longan peel powder;

[0045] (2) According to the solid-liquid ratio of 1:6 g / mL, longan peel powder was added to deionized water for steam distillation. First, the temperature was raised to 160°C at a rate of 5°C / min and maintained for 10 min. Then the temperature was lowered to 110°C and extracted for 2 h at normal pressure and condensation temperature of 8°C. The distillate was collected to obtain longan peel essential oil.

[0046] A repairing and whitening product, comprising the following raw materials in terms of mass percentage:

[0047]

[0048] The preparation method of the above-mentioned repair and whitening product is specifically as follows:

[0049] (1) 0.4% 1,3-butylene glycol, carbomer 980, sodium hyaluronate and glycerin are mixed and stirred to obtain phase A1;

[0050] Mix niacinamide, D-panthenol, lupinus root gum oil and erythritol to obtain phase A2;

[0051] Mix arginine and 3% deionized water to obtain phase B;

[0052] Mix cinnamic acid and 1.1% 1,3-butanediol and heat to 60°C, and stir at constant temperature until transparent to obtain phase C;

[0053] Add Centella asiatica extract and tetrahydropyrimidine carboxylic acid into 5% deionized water and stir well to obtain phase D;

[0054] Mix longan peel essential oil, transdermal peptide, 1,2-hexanediol and ethylhexylglycerin to obtain phase E;

[0055] (2) Add 86.17% deionized water into the main pot, stir and heat to 80°C, add phase A2 and phase A1 in sequence, homogenize at 3000HZ for 5 minutes, then stir at 80°C, 15HZ for 30 minutes, cool to 60°C, add phase B, phase C and phase D, continue stirring at 10HZ until the temperature drops to below 45°C, add phase E, stir evenly, cool to below 38°C, filter the material through 100 mesh to obtain a repairing and whitening product.

[0056] Example 3

[0057] A method for extracting longan peel essential oil, the specific steps comprising:

[0058] (1) washing longan peel, drying at 70° C., and crushing to obtain longan peel powder;

[0059] (2) According to the solid-liquid ratio of 1:10 g / mL, longan peel powder was added to deionized water for steam distillation. First, the temperature was raised to 180°C at a heating rate of 5°C / min and maintained for 15 min. Then the temperature was lowered to 130°C and extracted for 5 h at normal pressure and condensation temperature of 8°C. The distillate was collected to obtain longan peel essential oil.

[0060] A repairing and whitening product, comprising the following raw materials in terms of mass percentage:

[0061]

[0062] The preparation method of the above-mentioned repair and whitening product is specifically as follows:

[0063] (1) 1.2% 1,3-butylene glycol, carbomer 980, sodium hyaluronate and glycerin are mixed and stirred to obtain phase A1;

[0064] Mix niacinamide, D-panthenol, lupinus root gum oil and erythritol to obtain phase A2;

[0065] Mix arginine and 3% deionized water to obtain phase B;

[0066] Mix cinnamic acid and 2.8% 1,3-butanediol, heat to 60°C, and stir at constant temperature until transparent to obtain phase C;

[0067] Add Centella asiatica extract and tetrahydropyrimidine carboxylic acid into 5% deionized water and stir well to obtain phase D;

[0068] Mix longan peel essential oil, transdermal peptide, 1,2-hexanediol and ethylhexylglycerin to obtain phase E;

[0069] (2) Add 75.4% deionized water to the main pot, stir and heat to 90°C, add phase A2 and phase A1 in sequence, homogenize at 3000HZ for 5 minutes, then stir at 90°C, 25HZ for 35 minutes, cool to 60°C, add phase B, phase C and phase D, continue stirring at 15HZ until the temperature drops to below 45°C, add phase E, stir evenly, cool to below 38°C, filter the material through 100 mesh to obtain a repairing and whitening product.

[0070] Comparative Example 1

[0071] The difference from Example 1 is that the solid-liquid ratio of longan peel powder to deionized water is 1:5 g / mL or 1:11 g / mL, and the rest is consistent with Example 1.

[0072] Comparative Example 2

[0073] The difference from Example 1 is that the extraction time of steam distillation is 6 hours, and the others are consistent with Example 1.

[0074] Comparative Example 3

[0075] The difference from Example 1 is that the extraction site for steam distillation is the longan kernel, and the rest is consistent with Example 1.

[0076] That is, the extraction method of the longan fruit core essential oil of this comparative example, specifically comprises the following steps:

[0077] (1) washing longan fruit cores, drying at a constant temperature of 60° C., and crushing to obtain longan fruit core powder;

[0078] (2) According to the solid-liquid ratio of 1:7 g / mL, longan kernel powder was added into deionized water for steam distillation. The temperature was first raised to 170 °C at a rate of 5 °C / min and maintained for 13 min. Then the temperature was lowered to 120 °C and extracted for 4 h at normal pressure and condensation temperature of 8 °C. The distillate was collected to obtain longan kernel essential oil.

[0079] Test Example 1

[0080] According to the extraction methods of Examples 1-3 and Comparative Examples 1-3, the essential oil yield was calculated, where essential oil yield = mass of essential oil / mass of powder × 100%. The results are shown in Table 1.

[0081] Table 1

[0082] sample Essential oil yield / % Example 1 0.20 Example 2 0.18 Example 3 0.19 Comparative Example 1 (material-liquid ratio 1:5 g / mL) 0.12 Comparative Example 1 (material-liquid ratio 1:11 g / mL) 0.16 Comparative Example 2 0.16 Comparative Example 3 0.05

[0083] It can be seen from Table 1 that, compared with Comparative Examples 1-3, the extraction methods of Examples 1-3 of the present invention have a high yield of longan peel essential oil.

[0084] Comparative Example 4

[0085] The longan flower extract is obtained by the method in CN109276493A.

[0086] Comparative Example 5

[0087] Longan fruit core polyphenols are obtained by the method in CN104473825A.

[0088] Comparative Example 6

[0089] The longan peel extract is obtained by the method in CN118648632A.

[0090] Comparative Example 7

[0091] The difference from Example 1 is that alcohol extraction is adopted, and the rest is the same as Example 1.

[0092] That is, the extraction method of the longan peel essential oil of this comparative example, specifically comprising the following steps:

[0093] (1) washing longan peel, drying at a constant temperature of 60° C., and crushing to obtain longan peel powder;

[0094] (2) According to the solid-liquid ratio of 1:25 g / mL, the longan peel powder was added to a 50% ethanol solution, ultrasonicated at 60° C. for 30 min, cooled and filtered to obtain a longan peel extract.

[0095] Test Example 2-Repair Efficacy Detection

[0096] Detection principle: By promoting the migration of skin cells, chemotaxis of fibroblasts and activating cell vitality, it can help restore tissue structure and function, form new skin, and achieve the effect of promoting wound healing. Therefore, a healing and repair experiment was conducted on HSF human skin fibroblasts to test the repair effect of the samples.

[0097] Detection method: HSF cells were placed at 37°C, 5% CO2 Routine culture was performed in an incubator. HSF cells in the logarithmic growth phase were plated onto 6-well plates and cultured overnight. When the cell confluence reached 80%, vertical scratches were made and photographed. The sample (repair and whitening product) was diluted to a concentration of 5% as the experimental group, and water was used as the control group. The cells were cultured in the incubator for 12 hours. After 12 hours, photographs were taken again to calculate the cell migration (healing) area.

[0098] Cell healing rate = (initial scratch area - scratch area after 12 hours) / initial scratch area × 100%

[0099] The results are shown in Table 2.

[0100] Table 2

[0101] sample Cell healing rate Control group (water) 45.018％ Example 1 87.947※ Example 2 86.487※ Example 3 87.124※ Comparative Example 4 80.141※ Comparative Example 5 54.761※ Comparative Example 6 70.658※ Comparative Example 7 76.987※

[0102] Note: ※Compared with the control group, p<0.01

[0103] It can be seen from Table 2 that, compared with the control group, the cell healing rates of Examples 1-3 of the present invention are significantly increased and are better than those of Comparative Examples 4-7, indicating that the products of the present invention have good repair capabilities.

[0104] Test Example 3-Whitening Efficacy Test

[0105] Detection principle: A375 human malignant melanoma cells produce melanin after ultraviolet irradiation. Melanin particles move up to the stratum corneum along with epidermal cells, thus affecting the color of the skin and forming spots, causing visual darkening. Inhibiting melanin production can achieve a whitening effect. Therefore, this characteristic is used to test the whitening effect.

[0106] Detection method: A375 cells were placed at 37°C, 5% CO 2 Routine culture was performed in an incubator, and A375 cells in the logarithmic growth phase were plated into 6-well plates with 3 replicates in each group. The cells were cultured adherently for 4 hours and UV irradiated at 0 and 12 hours. The sample (repair and whitening product) was diluted to a concentration of 5% as the experimental group, and water was used as the control group. The cells were cultured in an incubator for 24 hours, and the cells were lysed. The melanin-specific absorbance was detected by an ELISA instrument, and the melanin content inhibition rate was calculated.

[0107] Melanin content inhibition rate = (1-absorbance of experimental group / absorbance of control group) × 100%

[0108] The results are shown in Table 3.

[0109] Table 3

[0110] sample Melanin content inhibition rate / % Control group (water) 0 Example 1 54.272※ Example 2 53.874※ Example 3 54.257※ Comparative Example 4 46.166※ Comparative Example 5 22.258※ Comparative Example 6 29.415※ Comparative Example 7 35.683※

[0111] Note: ※Compared with the control group, p<0.01

[0112] As can be seen from Table 3, Examples 1-3 of the present invention have good melanin content inhibition rates and are superior to Comparative Examples 4-7, indicating that the products of the present invention have good whitening ability.

[0113] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. Application of longan peel essential oil in preparing repairing and whitening products, characterized in that: The extraction method of the longan peel essential oil is: (1) cleaning longan peel, drying, and crushing to obtain longan peel powder; (2) Adding longan peel powder to deionized water, extracting by steam distillation, collecting the distillate, and condensing at normal pressure to obtain longan peel essential oil.

2. The use according to claim 1, characterized in that In step (2) of the method for extracting longan peel essential oil, the solid-liquid ratio of the longan peel powder to deionized water is 1:6-10 g / mL.

3. The use according to claim 1, characterized in that In step (2) of the method for extracting longan peel essential oil, the conditions for the steam distillation are specifically as follows: first, the temperature is raised to 160-180° C. at a heating rate of 5° C. / min and maintained for 10-15 min, and then the temperature is lowered to 110-130° C. and maintained for 2-5 h.

4. The use according to claim 1, characterized in that The repair and whitening product comprises the following raw materials in percentage by mass: 0.1-0.3% longan peel essential oil, 1.5-4% 1,3-butylene glycol, 1-3% glycerol, 0.5-2% niacinamide, 0.3-0.6% 1,2-hexanediol, 0.4-1.2% erythritol, 0.3-1% D-panthenol, 0.3-1% lubric acid oil, 0.3-0.6% cinnamone, 0.5-1% tetrahydromethylpyrimidine carboxylic acid, 0.2-0.5% carbomer 980, 0.2-0.5% arginine, 0.1-0.5% sodium hyaluronate, 0.1-0.3% transdermal polypeptide, 0.02-0.05% Centella asiatica extract, 0.01-0.05% ethylhexylglycerin, and the rest is deionized water.

5. The use according to claim 4, characterized in that The total glycoside content of the Centella asiatica extract is 90%-95%; the molecular weight of the sodium hyaluronate is 130w-150w Da.

6. The use according to claim 4, characterized in that The preparation method of the repair and whitening product is specifically as follows: (1) 0.4%-1.2% 1,3-butylene glycol, carbomer 980, sodium hyaluronate and glycerin are mixed and stirred to obtain phase A1; Mix niacinamide, D-panthenol, lupinus root gum oil and erythritol to obtain phase A2; Mix arginine and 2%-3% deionized water to obtain phase B; Mix cinnamone and the remaining 1,3-butanediol and heat to 60°C, and stir at constant temperature until transparent to obtain phase C; Add the Centella asiatica extract and tetrahydropyrimidine carboxylic acid into 2%-5% deionized water and stir evenly to obtain phase D; Mix longan peel essential oil, transdermal peptide, 1,2-hexanediol and ethylhexylglycerin to obtain phase E; (2) Add the remaining deionized water into the main pot, stir and heat to 80-90°C, add phase A2 and phase A1 in sequence, homogenize, stir at 80-90°C for 30-35 minutes, cool to 60°C, add phase B, phase C and phase D, continue stirring and cool to below 45°C, add phase E, stir evenly, and cool to below 38°C to obtain the target repair and whitening product.

7. The use according to claim 6, characterized in that Step (2) of the preparation method of the repair and whitening product is specifically as follows: add the remaining deionized water into the main pot, stir and heat to 80-90°C, add phase A2 and phase A1 in sequence, homogenize at 3000HZ for 5 minutes, then stir at 80-90°C and 15-25HZ for 30-35 minutes, cool to 60°C, add phase B, phase C and phase D, continue stirring at 10-15HZ and cool to below 45°C, add phase E, stir evenly, cool to below 38°C, filter the material through 100 mesh to obtain the target repair and whitening product.

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